LOC101078615 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101078615
Entrez Gene ID	101078615
Full Name	solute carrier family 8 member A2
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003968703.2	XP_003968752.2	sodium/calcium exchanger 2
XM_003968703.3	XP_003968752.3	sodium/calcium exchanger 2

Pathways from BioSystems
Gene Ontology

The following LOC101078615 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101078615 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo31238	XM_003968703.2 Latest version!	Takifugu rubripes solute carrier family 8 (sodium/calcium exchanger), member 2 (slc8a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OTo45162	XM_003968703.3 Latest version!	Takifugu rubripes sodium/calcium exchanger 2 (LOC101078615), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101078615 ( XM_003968703.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003968752.2 Takifugu rubripes solute carrier family 8 (sodium/calcium exchanger), member 2 (slc8a2), mRNA.

Price & Availability↑

CloneID	OTo31238
Clone ID Related Accession (Same CDS sequence)	XM_003968703.2
Accession Version	XM_003968703.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	sodium/calcium exchanger 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071046.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003968703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACATGT TCCTGGGTGT GTCCATCATC GCCGACCGCT TCATGGCCTC CATTGAAGTC 
ATCACATCAC AGGAGAAGGA AGTGACCATC ACTTTGCCTA GCGGGGAAAC ATCTGTTGCT 
ACGGTTCGAA TCTGGAACGA AACGGTGTCC AACTTGACCC TCATGGCCCT GGGCTCCAGC 
GCTCCGGAGA TCCTGCTCTC CCTTATAGAG GTGTGCGGCC ACAAATTTGA AGCTGGTGAA 
CTGGGACCAG GGACCATCGT TGGCAGCGCC GCCTTTAACA TGTTTGTGAT CATTGGGATA 
TGCGTGTGGA CCATCCCCAA AGGCGAGAGC CGCAAGATCA AACACCTGCG GGTGTTCTTC 
ATCACCGCCT TCTGGAGCAT CTTCGCCTAC ATCTGGCTCT TCCTCATCCT GTCAGTCATG 
ACGCCTGGCG TTGTGGAGGT GTGGGAGGCC CTCGTGACCT TGCTCTTCTT CCCCGTGTGT 
GTCATCCTGG CCTGGATCGC CGACCGCCGC CTGCTCTTCT ACAAGTACAT GGGCAAGCAT 
TACCGCGCCG ACAAGCGGCA CGGCATCGTC GTGGAGACCG AGGGAGACTT GACTCCCAAC 
AAGGGGGGCA TGGAGATGAT GGTGGATGGC AAGATCCCAC CGAGAGGGGG CACCGTGGTG 
CCCGCTGAAA ACTGCGGGGT CGGAGGTCAG GACGGCACAG TCGGCGCTGC AGCAAACAAC 
ATCAACATGC CGAACTCCAA CAGCGCCACC ATGGTGGAGA GCAGCAAGGA GCTGGACGAG 
AGCCGGAGAG AGGTGATACG TATCCTGAAG GAGCTGAAGC AGAAATACCC AGAAAAAGAG 
CTGGACCAGC TGGTGGAGTT GGCCAACTAC TACGCACTGC TGCACCAGCA GAAGAGCCGG 
GCCTTCTACC GTATTCAGGC GACGCGAATG ATGATCGGGG CTGGCAACGT CCTGAAGAAG 
CACGCGGCCG ACCACGCGCG GCGCACCGCG GTGACGGACG AGGAAGCGCC AGAGGAAGAC 
GACATGGAGA TCTGCAGCCG CATCTCTTTT GAGAGCGCCC ACAGTCAGTG CATGGAGAAC 
TGCGGCATTC TGACGCTGGC TGTCGTGTGT CAAGGCGGTT TGGGACAGAA CACCTTCTAC 
GTGGACTACA GGACAGAGGA CGGGTCGGCA AACGCCGGGT CAGATTACCA TTACAGTGAG 
GGAACACTGG TGTTCAAACC CGGAGATACA CGGAAAGAGA TTAAGGTCGG CATCATCGAT 
GATGACATAT TCGAGGAGGA CGAGCACTTC TTTGTGCGCC TCCTCAACCT GCGTGTCGGC 
GATGCCGAGG GGATGTTTGA GAGCGACGAC GTCGGCGCGA CCCCCAAGGC CCGTTTGGTC 
GAGCCGCTGG TCGCCACGGT GACCATCTTG GACGACGACC ACGCCGGCAT CTTCACGTTC 
AGCGAGCGCT TGGTCCGGGT GAGCGAGAGC GTGGGCACCA TGGAGGTGAC CGTGGTGCGT 
AACTCGGGAG CCCGGGGGAC CGTCATCCTG CCGTACCACA CCGAGTCAGG GACCGCCAAA 
GCCGGGGCAG ACTACGAGGA CGCCCGAGGA GAGCTGGAGT TCACCAACGA CCAGACAATT 
CAGTCTTTCC AGGTGAGGAT AATTGATGAT GAAGAATATG AAAAACATGA AAACTTCTTC 
ATTGTGCTGG AGGAGCCACG CTGGCTTAAG AGAGGAATCT CAGCTCTGCT GCTCAACCAA 
GAGGGGGCCG AGGGTCAGAT GAGCGCTGAG GAGGAGGAAG CCCGCCGGAT AGCAGAGATG 
GGGAAGCCCA TTCTGGGAGA ACACAGCCGC CTGGAGGTGG TTATCGAGGA GTCGTACGAG 
TTTAAGAGCA CCGTTGACAA GCTCATTAAG AAGACCAACT TGGCTCTGGT GATCGGGACA 
CACTCCTGGA GGGAGCAGTT CGTGGAGGCA GTCACAGTCA GCGCAGGCGA TGGTGACGAT 
GACGAGGAAG GCCGAGGGGA CCGCCTTCCC TCCTGTTATG ATTACGTCAT GCACTTTCTC 
ACCGTTTTCT GGAAGGTTCT GTTTGCCTGC GTTCCACCCA CGGAGTACTG GAACGGTTGG 
GCCTGCTTCC TGGTGTCCAT CAGCATCATC GGCCTCCTCA CTGCCGTCAT TGGAGATCTG 
GCGTCGCACT TTGGCTGCAC CGTCGGCCTC CGAGACACCG TGACCGCCGT GGTGTTTGTG 
GCGTTGGGAA CTTCTGTTCC AGACACATTT GCTAGCAAAG TGGCGGCCAT GCAGGACCAG 
CACGCCGACG CCTCTGTCGG CAACGTCACC GGCAGCAACG CTGTCAACGT GTTCCTGGGC 
ATCGGCTTGG CTTGGACGGT GGCGGCGGTC TACTGGAAGT TTGAAGGGAA GGAGTTCAAG 
GTCGACCCCG GCTCGCTGGC GTTCTCGGTC ACGCTGTTCA CCATCTTTGC TTTCATCGCC 
ATGGCTGTTC TCCTGTTCAG ACGCCGGCCA TCCATCGGAG GAGAGCTGGG AGGTCCGAGG 
ATGTCCAGAC TGTTGACCAC GCTGCTCTTA CTCTTCCTGT GGTTCCTCTA CATCCTTTTT 
TCCAGCCTGG AAGCCTACTG CCACATCGAG GGCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003968752.2
CDS	16..2634
Translation	MYMFLGVSIIADRFMASIEVITSQEKEVTITLPSGETSVATVRIWNETVSNLTLMALGSSAPEILLSLIEVCGHKFEAGELGPGTIVGSAAFNMFVIIGICVWTIPKGESRKIKHLRVFFITAFWSIFAYIWLFLILSVMTPGVVEVWEALVTLLFFPVCVILAWIADRRLLFYKYMGKHYRADKRHGIVVETEGDLTPNKGGMEMMVDGKIPPRGGTVVPAENCGVGGQDGTVGAAANNINMPNSNSATMVESSKELDESRREVIRILKELKQKYPEKELDQLVELANYYALLHQQKSRAFYRIQATRMMIGAGNVLKKHAADHARRTAVTDEEAPEEDDMEICSRISFESAHSQCMENCGILTLAVVCQGGLGQNTFYVDYRTEDGSANAGSDYHYSEGTLVFKPGDTRKEIKVGIIDDDIFEEDEHFFVRLLNLRVGDAEGMFESDDVGATPKARLVEPLVATVTILDDDHAGIFTFSERLVRVSESVGTMEVTVVRNSGARGTVILPYHTESGTAKAGADYEDARGELEFTNDQTIQSFQVRIIDDEEYEKHENFFIVLEEPRWLKRGISALLLNQEGAEGQMSAEEEEARRIAEMGKPILGEHSRLEVVIEESYEFKSTVDKLIKKTNLALVIGTHSWREQFVEAVTVSAGDGDDDEEGRGDRLPSCYDYVMHFLTVFWKVLFACVPPTEYWNGWACFLVSISIIGLLTAVIGDLASHFGCTVGLRDTVTAVVFVALGTSVPDTFASKVAAMQDQHADASVGNVTGSNAVNVFLGIGLAWTVAAVYWKFEGKEFKVDPGSLAFSVTLFTIFAFIAMAVLLFRRRPSIGGELGGPRMSRLLTTLLLLFLWFLYILFSSLEAYCHIEGF

Target ORF information:

RefSeq Version	XM_003968703.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes solute carrier family 8 (sodium/calcium exchanger), member 2 (slc8a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003968703.2

ORF Insert Sequence:

ATGTACATGT TCCTGGGTGT GTCCATCATC GCCGACCGCT TCATGGCCTC CATTGAAGTC 
ATCACATCAC AGGAGAAGGA AGTGACCATC ACTTTGCCTA GCGGGGAAAC ATCTGTTGCT 
ACGGTTCGAA TCTGGAACGA AACGGTGTCC AACTTGACCC TCATGGCCCT GGGCTCCAGC 
GCTCCGGAGA TCCTGCTCTC CCTTATAGAG GTGTGCGGCC ACAAATTTGA AGCTGGTGAA 
CTGGGACCAG GGACCATCGT TGGCAGCGCC GCCTTTAACA TGTTTGTGAT CATTGGGATA 
TGCGTGTGGA CCATCCCCAA AGGCGAGAGC CGCAAGATCA AACACCTGCG GGTGTTCTTC 
ATCACCGCCT TCTGGAGCAT CTTCGCCTAC ATCTGGCTCT TCCTCATCCT GTCAGTCATG 
ACGCCTGGCG TTGTGGAGGT GTGGGAGGCC CTCGTGACCT TGCTCTTCTT CCCCGTGTGT 
GTCATCCTGG CCTGGATCGC CGACCGCCGC CTGCTCTTCT ACAAGTACAT GGGCAAGCAT 
TACCGCGCCG ACAAGCGGCA CGGCATCGTC GTGGAGACCG AGGGAGACTT GACTCCCAAC 
AAGGGGGGCA TGGAGATGAT GGTGGATGGC AAGATCCCAC CGAGAGGGGG CACCGTGGTG 
CCCGCTGAAA ACTGCGGGGT CGGAGGTCAG GACGGCACAG TCGGCGCTGC AGCAAACAAC 
ATCAACATGC CGAACTCCAA CAGCGCCACC ATGGTGGAGA GCAGCAAGGA GCTGGACGAG 
AGCCGGAGAG AGGTGATACG TATCCTGAAG GAGCTGAAGC AGAAATACCC AGAAAAAGAG 
CTGGACCAGC TGGTGGAGTT GGCCAACTAC TACGCACTGC TGCACCAGCA GAAGAGCCGG 
GCCTTCTACC GTATTCAGGC GACGCGAATG ATGATCGGGG CTGGCAACGT CCTGAAGAAG 
CACGCGGCCG ACCACGCGCG GCGCACCGCG GTGACGGACG AGGAAGCGCC AGAGGAAGAC 
GACATGGAGA TCTGCAGCCG CATCTCTTTT GAGAGCGCCC ACAGTCAGTG CATGGAGAAC 
TGCGGCATTC TGACGCTGGC TGTCGTGTGT CAAGGCGGTT TGGGACAGAA CACCTTCTAC 
GTGGACTACA GGACAGAGGA CGGGTCGGCA AACGCCGGGT CAGATTACCA TTACAGTGAG 
GGAACACTGG TGTTCAAACC CGGAGATACA CGGAAAGAGA TTAAGGTCGG CATCATCGAT 
GATGACATAT TCGAGGAGGA CGAGCACTTC TTTGTGCGCC TCCTCAACCT GCGTGTCGGC 
GATGCCGAGG GGATGTTTGA GAGCGACGAC GTCGGCGCGA CCCCCAAGGC CCGTTTGGTC 
GAGCCGCTGG TCGCCACGGT GACCATCTTG GACGACGACC ACGCCGGCAT CTTCACGTTC 
AGCGAGCGCT TGGTCCGGGT GAGCGAGAGC GTGGGCACCA TGGAGGTGAC CGTGGTGCGT 
AACTCGGGAG CCCGGGGGAC CGTCATCCTG CCGTACCACA CCGAGTCAGG GACCGCCAAA 
GCCGGGGCAG ACTACGAGGA CGCCCGAGGA GAGCTGGAGT TCACCAACGA CCAGACAATT 
CAGTCTTTCC AGGTGAGGAT AATTGATGAT GAAGAATATG AAAAACATGA AAACTTCTTC 
ATTGTGCTGG AGGAGCCACG CTGGCTTAAG AGAGGAATCT CAGCTCTGCT GCTCAACCAA 
GAGGGGGCCG AGGGTCAGAT GAGCGCTGAG GAGGAGGAAG CCCGCCGGAT AGCAGAGATG 
GGGAAGCCCA TTCTGGGAGA ACACAGCCGC CTGGAGGTGG TTATCGAGGA GTCGTACGAG 
TTTAAGAGCA CCGTTGACAA GCTCATTAAG AAGACCAACT TGGCTCTGGT GATCGGGACA 
CACTCCTGGA GGGAGCAGTT CGTGGAGGCA GTCACAGTCA GCGCAGGCGA TGGTGACGAT 
GACGAGGAAG GCCGAGGGGA CCGCCTTCCC TCCTGTTATG ATTACGTCAT GCACTTTCTC 
ACCGTTTTCT GGAAGGTTCT GTTTGCCTGC GTTCCACCCA CGGAGTACTG GAACGGTTGG 
GCCTGCTTCC TGGTGTCCAT CAGCATCATC GGCCTCCTCA CTGCCGTCAT TGGAGATCTG 
GCGTCGCACT TTGGCTGCAC CGTCGGCCTC CGAGACACCG TGACCGCCGT GGTGTTTGTG 
GCGTTGGGAA CTTCTGTTCC AGACACATTT GCTAGCAAAG TGGCGGCCAT GCAGGACCAG 
CACGCCGACG CCTCTGTCGG CAACGTCACC GGCAGCAACG CTGTCAACGT GTTCCTGGGC 
ATCGGCTTGG CTTGGACGGT GGCGGCGGTC TACTGGAAGT TTGAAGGGAA GGAGTTCAAG 
GTCGACCCCG GCTCGCTGGC GTTCTCGGTC ACGCTGTTCA CCATCTTTGC TTTCATCGCC 
ATGGCTGTTC TCCTGTTCAG ACGCCGGCCA TCCATCGGAG GAGAGCTGGG AGGTCCGAGG 
ATGTCCAGAC TGTTGACCAC GCTGCTCTTA CTCTTCCTGT GGTTCCTCTA CATCCTTTTT 
TCCAGCCTGG AAGCCTACTG CCACATCGAG GGCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078615 ( XM_003968703.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003968752.3 Takifugu rubripes sodium/calcium exchanger 2 (LOC101078615), mRNA.

Price & Availability↑

CloneID	OTo45162
Clone ID Related Accession (Same CDS sequence)	XM_003968703.3
Accession Version	XM_003968703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	sodium/calcium exchanger 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042295.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003968703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTGT CCGCCTCTCC TGCCCCCTTT CTCTTCCTCC TGTTCATCTT CCTCCTGCCC 
CTGCCTCCCT GCCAGCCCGA GTCCCAACGT AGCGAGCCCA GCAGCAGCCC TGACCTGTGG 
ACGCTCTCCC CCTCCCTGTT CTCCTTCTCC TTCTCTCCCT CCGTGTCTTA CGGTAAAAGC 
AACACGTCTT CCCCAAAAAG CAAGTGTGAT CGAAAAGTGA GCAACTGCAC AAACGGCATC 
CTGCTTCCTG TCTGGGAGCC CATCCAGCCT GAACTGGGGG AAAAGGTGGC CAGAGCTGTG 
GTCTACTTTG TCTCCCTCAT GTACATGTTC CTGGGTGTGT CCATCATCGC CGACCGCTTC 
ATGGCCTCCA TTGAAGTCAT CACATCACAG GAGAAGGAAG TGACCATCAC TTTGCCTAGC 
GGGGAAACAT CTGTTGCTAC GGTTCGAATC TGGAACGAAA CGGTGTCCAA CTTGACCCTC 
ATGGCCCTGG GCTCCAGCGC TCCGGAGATC CTGCTCTCCC TTATAGAGGT GTGCGGCCAC 
AAATTTGAAG CTGGTGAACT GGGACCAGGG ACCATCGTTG GCAGCGCCGC CTTTAACATG 
TTTGTGATCA TTGGGATATG CGTGTGGACC ATCCCCAAAG GCGAGAGCCG CAAGATCAAA 
CACCTGCGGG TGTTCTTCAT CACCGCCTTC TGGAGCATCT TCGCCTACAT CTGGCTCTTC 
CTCATCCTGT CAGTCATGAC GCCTGGCGTT GTGGAGGTGT GGGAGGCCCT CGTGACCTTG 
CTCTTCTTCC CCGTGTGTGT CATCCTGGCC TGGATCGCCG ACCGCCGCCT GCTCTTCTAC 
AAGTACATGG GCAAGCATTA CCGCGCCGAC AAGCGGCACG GCATCGTCGT GGAGACCGAG 
GGAGACTTGA CTCCCAACAA GGGGGGCATG GAGATGATGG TGGATGGCAA GATCCCACCG 
AGAGGGGGCA CCGTGGTGCC CGCTGAAAAC TGCGGGGTCG GAGGTCAGGA CGGCACAGTC 
GGCGCTGCAG CAAACAACAT CAACATGCCG AACTCCAACA GCGCCACCAT GGTGGAGAGC 
AGCAAGGAGC TGGACGAGAG CCGAAGAGAG GTGATACGTA TCCTGAAGGA GCTGAAGCAG 
AAATACCCAG AAAAAGAGCT GGACCAGCTG GTGGAGCTGG CCAACTACTA CGCACTGCTG 
CACCAGCAGA AGAGCCGGGC CTTCTACCGT ATTCAGGCGA CGCGAATGAT GATCGGGGCT 
GGCAACGTCC TGAAGAAGCA CGCGGCCGAC CACGCGCGGC GCACCGCGGT GACGGACGAG 
GAAGCGCCAG AGGAAGACGA CATGGAGATC TGCAGCCGCA TCTCTTTTGA GAGCGCCCAC 
AGTCAGTGCA TGGAGAACTG CGGCATTCTG ACGCTGGCTG TCGTGTGTCA AGGCGGTTTG 
GGACAGAACA CCTTCTACGT GGACTACAGG ACAGAGGACG GGTCGGCAAA CGCCGGGTCA 
GATTACCATT ACAGTGAGGG AACACTGGTG TTCAAACCCG GAGATACACG GAAAGAGATT 
AAGGTCGGCA TCATCGATGA TGACATATTC GAGGAGGACG AGCACTTCTT TGTGCGCCTC 
CTCAACCTGC GTGTCGGCGA TGCCGAGGGG ATGTTTGAGA GCGACGACGT CGGCGCGACC 
CCCAAGGCCC GTTTGGTCGA GCCGCTGGTC GCCACGGTGA CCATCTTGGA CGACGACCAC 
GCCGGCATCT TCACGTTCAG CGAGCGCTTG GTCCGGGTGA GCGAGAGCGT GGGCACCATG 
GAGGTGACCG TGGTGCGTAA CTCGGGAGCC CGGGGGACCG TCATCCTGCC GTACCACACC 
GAGTCAGGGA CCGCCAAAGC CGGGGCAGAC TACGAGGACG CCCGAGGAGA GCTGGAGTTC 
ACCAACGACC AGACAATTCA GTCTTTCCAG GTGAGGATAA TTGATGATGA AGAATATGAA 
AAACATGAAA ACTTCTTCAT TGTGCTGGAG GAGCCACGCT GGCTTAAGAG AGGAATCTCA 
GCTCTGCTGC TCAACCAAGA GGGGGCCGAG GGTCAGATGA GCGCTGAGGA GGAGGAAGCC 
CGCCGGATAG CAGAGATGGG GAAGCCCATT CTGGGAGAAC ACAGCCGCCT GGAGGTGGTT 
ATCGAGGAGT CGTACGAGTT TAAGAGCACC GTTGACAAGC TCATTAAGAA GACCAACTTG 
GCTCTGGTGA TCGGGACACA CTCCTGGAGG GAGCAGTTCG TGGAGGCAGT CACAGTCAGC 
GCAGGCGATG GTGACGATGA CGAGGAAGGC CGAGGGGACC GCCTTCCCTC CTGTTATGAT 
TACGTCATGC ACTTTCTCAC CGTTTTCTGG AAGGTTCTGT TTGCCTGCGT TCCACCCACG 
GAGTACTGGA ACGGTTGGGC CTGCTTCCTG GTGTCCATCA GCATCATCGG CCTCCTCACT 
GCCGTCATTG GAGATCTGGC GTCGCACTTT GGCTGCACCG TCGGCCTCCG AGACACCGTG 
ACCGCCGTGG TGTTTGTGGC GTTGGGAACT TCTGTTCCAG ACACATTTGC TAGCAAAGTG 
GCGGCCATGC AGGACCAGCA CGCCGACGCC TCTGTCGGCA ACGTCACCGG CAGCAACGCT 
GTCAACGTGT TCCTGGGCAT CGGCTTGGCT TGGACGGTGG CGGCGGTCTA CTGGAAGTTT 
GAAGGGAAGG AGTTCAAGGT CGACCCCGGC TCGCTGGCGT TCTCGGTCAC GCTGTTCACC 
ATCTTTGCTT TCATCGCCAT GGCTGTTCTC CTGTTCAGAC GCCGGCCATC CATCGGAGGA 
GAGCTGGGAG GTCCGAGGAT GTCCAGACTG TTGACCACGC TGCTCTTACT CTTCCTGTGG 
TTCCTCTACA TCCTTTTTTC CAGCCTGGAA GCCTACTGCC ACATCGAGGG CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003968752.3
CDS	310..3246
Translation	MPLSASPAPFLFLLFIFLLPLPPCQPESQRSEPSSSPDLWTLSPSLFSFSFSPSVSYGKSNTSSPKSKCDRKVSNCTNGILLPVWEPIQPELGEKVARAVVYFVSLMYMFLGVSIIADRFMASIEVITSQEKEVTITLPSGETSVATVRIWNETVSNLTLMALGSSAPEILLSLIEVCGHKFEAGELGPGTIVGSAAFNMFVIIGICVWTIPKGESRKIKHLRVFFITAFWSIFAYIWLFLILSVMTPGVVEVWEALVTLLFFPVCVILAWIADRRLLFYKYMGKHYRADKRHGIVVETEGDLTPNKGGMEMMVDGKIPPRGGTVVPAENCGVGGQDGTVGAAANNINMPNSNSATMVESSKELDESRREVIRILKELKQKYPEKELDQLVELANYYALLHQQKSRAFYRIQATRMMIGAGNVLKKHAADHARRTAVTDEEAPEEDDMEICSRISFESAHSQCMENCGILTLAVVCQGGLGQNTFYVDYRTEDGSANAGSDYHYSEGTLVFKPGDTRKEIKVGIIDDDIFEEDEHFFVRLLNLRVGDAEGMFESDDVGATPKARLVEPLVATVTILDDDHAGIFTFSERLVRVSESVGTMEVTVVRNSGARGTVILPYHTESGTAKAGADYEDARGELEFTNDQTIQSFQVRIIDDEEYEKHENFFIVLEEPRWLKRGISALLLNQEGAEGQMSAEEEEARRIAEMGKPILGEHSRLEVVIEESYEFKSTVDKLIKKTNLALVIGTHSWREQFVEAVTVSAGDGDDDEEGRGDRLPSCYDYVMHFLTVFWKVLFACVPPTEYWNGWACFLVSISIIGLLTAVIGDLASHFGCTVGLRDTVTAVVFVALGTSVPDTFASKVAAMQDQHADASVGNVTGSNAVNVFLGIGLAWTVAAVYWKFEGKEFKVDPGSLAFSVTLFTIFAFIAMAVLLFRRRPSIGGELGGPRMSRLLTTLLLLFLWFLYILFSSLEAYCHIEGF

Target ORF information:

RefSeq Version	XM_003968703.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes sodium/calcium exchanger 2 (LOC101078615), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003968703.3

ORF Insert Sequence:

ATGCCTCTGT CCGCCTCTCC TGCCCCCTTT CTCTTCCTCC TGTTCATCTT CCTCCTGCCC 
CTGCCTCCCT GCCAGCCCGA GTCCCAACGT AGCGAGCCCA GCAGCAGCCC TGACCTGTGG 
ACGCTCTCCC CCTCCCTGTT CTCCTTCTCC TTCTCTCCCT CCGTGTCTTA CGGTAAAAGC 
AACACGTCTT CCCCAAAAAG CAAGTGTGAT CGAAAAGTGA GCAACTGCAC AAACGGCATC 
CTGCTTCCTG TCTGGGAGCC CATCCAGCCT GAACTGGGGG AAAAGGTGGC CAGAGCTGTG 
GTCTACTTTG TCTCCCTCAT GTACATGTTC CTGGGTGTGT CCATCATCGC CGACCGCTTC 
ATGGCCTCCA TTGAAGTCAT CACATCACAG GAGAAGGAAG TGACCATCAC TTTGCCTAGC 
GGGGAAACAT CTGTTGCTAC GGTTCGAATC TGGAACGAAA CGGTGTCCAA CTTGACCCTC 
ATGGCCCTGG GCTCCAGCGC TCCGGAGATC CTGCTCTCCC TTATAGAGGT GTGCGGCCAC 
AAATTTGAAG CTGGTGAACT GGGACCAGGG ACCATCGTTG GCAGCGCCGC CTTTAACATG 
TTTGTGATCA TTGGGATATG CGTGTGGACC ATCCCCAAAG GCGAGAGCCG CAAGATCAAA 
CACCTGCGGG TGTTCTTCAT CACCGCCTTC TGGAGCATCT TCGCCTACAT CTGGCTCTTC 
CTCATCCTGT CAGTCATGAC GCCTGGCGTT GTGGAGGTGT GGGAGGCCCT CGTGACCTTG 
CTCTTCTTCC CCGTGTGTGT CATCCTGGCC TGGATCGCCG ACCGCCGCCT GCTCTTCTAC 
AAGTACATGG GCAAGCATTA CCGCGCCGAC AAGCGGCACG GCATCGTCGT GGAGACCGAG 
GGAGACTTGA CTCCCAACAA GGGGGGCATG GAGATGATGG TGGATGGCAA GATCCCACCG 
AGAGGGGGCA CCGTGGTGCC CGCTGAAAAC TGCGGGGTCG GAGGTCAGGA CGGCACAGTC 
GGCGCTGCAG CAAACAACAT CAACATGCCG AACTCCAACA GCGCCACCAT GGTGGAGAGC 
AGCAAGGAGC TGGACGAGAG CCGAAGAGAG GTGATACGTA TCCTGAAGGA GCTGAAGCAG 
AAATACCCAG AAAAAGAGCT GGACCAGCTG GTGGAGCTGG CCAACTACTA CGCACTGCTG 
CACCAGCAGA AGAGCCGGGC CTTCTACCGT ATTCAGGCGA CGCGAATGAT GATCGGGGCT 
GGCAACGTCC TGAAGAAGCA CGCGGCCGAC CACGCGCGGC GCACCGCGGT GACGGACGAG 
GAAGCGCCAG AGGAAGACGA CATGGAGATC TGCAGCCGCA TCTCTTTTGA GAGCGCCCAC 
AGTCAGTGCA TGGAGAACTG CGGCATTCTG ACGCTGGCTG TCGTGTGTCA AGGCGGTTTG 
GGACAGAACA CCTTCTACGT GGACTACAGG ACAGAGGACG GGTCGGCAAA CGCCGGGTCA 
GATTACCATT ACAGTGAGGG AACACTGGTG TTCAAACCCG GAGATACACG GAAAGAGATT 
AAGGTCGGCA TCATCGATGA TGACATATTC GAGGAGGACG AGCACTTCTT TGTGCGCCTC 
CTCAACCTGC GTGTCGGCGA TGCCGAGGGG ATGTTTGAGA GCGACGACGT CGGCGCGACC 
CCCAAGGCCC GTTTGGTCGA GCCGCTGGTC GCCACGGTGA CCATCTTGGA CGACGACCAC 
GCCGGCATCT TCACGTTCAG CGAGCGCTTG GTCCGGGTGA GCGAGAGCGT GGGCACCATG 
GAGGTGACCG TGGTGCGTAA CTCGGGAGCC CGGGGGACCG TCATCCTGCC GTACCACACC 
GAGTCAGGGA CCGCCAAAGC CGGGGCAGAC TACGAGGACG CCCGAGGAGA GCTGGAGTTC 
ACCAACGACC AGACAATTCA GTCTTTCCAG GTGAGGATAA TTGATGATGA AGAATATGAA 
AAACATGAAA ACTTCTTCAT TGTGCTGGAG GAGCCACGCT GGCTTAAGAG AGGAATCTCA 
GCTCTGCTGC TCAACCAAGA GGGGGCCGAG GGTCAGATGA GCGCTGAGGA GGAGGAAGCC 
CGCCGGATAG CAGAGATGGG GAAGCCCATT CTGGGAGAAC ACAGCCGCCT GGAGGTGGTT 
ATCGAGGAGT CGTACGAGTT TAAGAGCACC GTTGACAAGC TCATTAAGAA GACCAACTTG 
GCTCTGGTGA TCGGGACACA CTCCTGGAGG GAGCAGTTCG TGGAGGCAGT CACAGTCAGC 
GCAGGCGATG GTGACGATGA CGAGGAAGGC CGAGGGGACC GCCTTCCCTC CTGTTATGAT 
TACGTCATGC ACTTTCTCAC CGTTTTCTGG AAGGTTCTGT TTGCCTGCGT TCCACCCACG 
GAGTACTGGA ACGGTTGGGC CTGCTTCCTG GTGTCCATCA GCATCATCGG CCTCCTCACT 
GCCGTCATTG GAGATCTGGC GTCGCACTTT GGCTGCACCG TCGGCCTCCG AGACACCGTG 
ACCGCCGTGG TGTTTGTGGC GTTGGGAACT TCTGTTCCAG ACACATTTGC TAGCAAAGTG 
GCGGCCATGC AGGACCAGCA CGCCGACGCC TCTGTCGGCA ACGTCACCGG CAGCAACGCT 
GTCAACGTGT TCCTGGGCAT CGGCTTGGCT TGGACGGTGG CGGCGGTCTA CTGGAAGTTT 
GAAGGGAAGG AGTTCAAGGT CGACCCCGGC TCGCTGGCGT TCTCGGTCAC GCTGTTCACC 
ATCTTTGCTT TCATCGCCAT GGCTGTTCTC CTGTTCAGAC GCCGGCCATC CATCGGAGGA 
GAGCTGGGAG GTCCGAGGAT GTCCAGACTG TTGACCACGC TGCTCTTACT CTTCCTGTGG 
TTCCTCTACA TCCTTTTTTC CAGCCTGGAA GCCTACTGCC ACATCGAGGG CTTTTAA