bub1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	bub1
Entrez Gene ID	101077348
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011611794.1	XP_011610096.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
XM_011611791.2	XP_011610093.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_011611791.1	XP_011610093.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_011611793.1	XP_011610095.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
XM_011611794.2	XP_011610096.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
XM_011611792.2	XP_011610094.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
XM_011611793.2	XP_011610095.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
XM_011611792.1	XP_011610094.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2

Pathways from BioSystems
Gene Ontology

The following bub1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bub1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo51933	XM_011611791.2 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo51934	XM_011611792.2 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo51935	XM_011611793.2 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo51936	XM_011611794.2 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo30324	XM_011611791.1 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo30325	XM_011611792.1 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo30326	XM_011611793.1 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo30327	XM_011611794.1 Latest version!	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bub1 ( XM_011611791.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610093.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo51933
Clone ID Related Accession (Same CDS sequence)	XM_011611791.2
Accession Version	XM_011611791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042300.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011611791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACAATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CCACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCGGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTGAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTG GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610093.1
CDS	145..3726
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISAESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNMSQGGTGNLSHITPNTSLGFVQATPSRVLPSPTVNTREALDVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEGTTENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611791.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611791.2

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACAATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CCACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCGGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTGAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTG GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_011611792.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610094.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo51934
Clone ID Related Accession (Same CDS sequence)	XM_011611792.2
Accession Version	XM_011611792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042300.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011611792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGAAAGCAG AAACCCTTTA 
CAAAATTCTC ATGTAACCAA TCAGCTGATG TCTCACCAAG AGCAACATAA CAAGGCCTCT 
GGGGACTGTT CTTTCAAACA TCCCACCATT GAAACCATTG TCACAGTTTC CCGCTCTGAG 
ACTTCTGGGA CCATTAACTC CAGCCAGAGC TCCAACGTTC AGTTTGTGTC CGAGTACCTG 
AAGGATGAGC TGCTCTGTGA GGGGTCCGAG TTGTGTTTCG AGGAGGTCCG AGCGGCGAAA 
TACTTCCGTA AACTGGAGGA GCAACAGGAT GATCAGACAG TAGATGTTTT GTTTGCAGAA 
GAGCAGTTTA TGAGGGTGGA GGAGGAGAAC TTCATGAGCA TGAGAGATGC ACTGGGGACA 
ATCAAGCAGG CTCTGGGGGG TGTAAAGAGC CACATGGGGC AGACAGCTGT AGTGGAAACA 
TCTGCTGCCA TGAACATTAA TCCTACTCAG CATTCCACCG GGCCTCCTCA GTTATCCAGT 
CGGCGCAGCA GCCGGCGCTC GCTCGGTTTG AGGCTGCTCA CTGAGCCCAC CTTCATCCAC 
GAGGCCCTTG CTGTCGTCCC TGACCAATAT CAGGAGGAGA GAAAGGTCAC ATCCTCTGAC 
CCTGATGAGA GACTGTGTCA TCCAGCTCTA ACTGACAGGA GTGTCCGGTC ACCCACATCT 
GCACCGACGT CTGCATCTAC GGAACCGTCA CCTGTTCTTC AGGCAGCTGC GGCAGAACAG 
ATGGCTCCGT CACTGCGGGG GGTCTCATCT ACTGAGAAAG TTCTCCTTCA GGAGGACCCA 
GAGATCAACA CTGCTGCAGC CCAGGATGTG GCATGTCTCC CTGAACCCGA CGACAGACTC 
AACATGTCAC AGGGTGGGAC CGGTAACCTT TCTCACATTA CTCCCAACAC ATCACTGGGT 
TTTGTTCAGG CCACACCATC ACGGGTGCTG CCGTCTCCCA CAGTCAACAC ACGGGAAGCT 
CTGGATGTTA TCATGGACAT GTTCCAGGCG CCAACCCTCC TCGAGGACCC GTTCAACAAA 
ACATCGGCTT TTCCCTCGTC AGAGGTGGAG GCAGAACCGG CCCCACTTAA ACATGGAGGG 
GACTCGTATT TCACTAAGCC CCGCGCTGCT GCCTCGTTTA CTATCTTCCA GGATGAACAA 
GACAAAGAAA ACATCAGTGC CGCTGCTCCT CATCCTTCAC TCAGCAAATC TAAACCAATC 
AAAGCTCTGG CTGAGATACG GCAGGTTAAA AAATCAAACG AGACGCCTCC TGAGCTGATG 
CCGGACGAGA GCACCATGTG GGGGGCCCGC TACAACTCCC TCAACTCCCT GGCGGCGTGT 
CCCAACAGCA CCACCGACTT CGCCATGTTG GCCCAGTGTG TGTCCACACC GTTCGCATTC 
AAGACGCCTC ACAGCAGCAA CTTCTTCCAG GATGAAGGTA CAACAGAGAA CAGCGGTGGT 
GGTGACGGTG ATGACGACGA ATTTATGAGA CGTCAACCGA AAAAACTCAG CCCCATCATG 
GAGCAGAGTC CAGCTGAAGA AACTGCCATC AGTCACATGG CCCTGCCCTC TGACAGGCTC 
GGGACCATTG TAGGTGAGGG TCTTGCCTCA GCCCAGCTCT GCTCGACCTC CTCCACCACC 
CTGGTGCAGG CTCCGCCCCC TGGTGTGTTG TCCTTCAGAG ACCAGACCAT CTCCACCAGG 
ACTACATTGG ACACAGACCA AGAGCAGCCC CCCAAGTCCA AGAGTCCATT TTCAATCAAG 
AGAGAACCAT TCAAGATACT GGAGGATCTG GATGAACCGG CCCAGAATCC AGTCGGTGAT 
GTTCCCATGA GTCCAGAGGG GACCCTCAAA CCTGACTGGC TGAGCATCGG GAGCCCTGAG 
GTTCCACCGG AAACTGACCT GGATGCTTTC CTGAGCCCAT GTCGGCCAAA GCGCGCTGAT 
GTGCCCATGA CCCCAGAACC ACCGCTCTGC ACAGCTGTAC CCATGAGCCC AGCAACGGCG 
CCGCAGTCAG ACGGAGGCCG GTGCTTGGAC GTGTCCATGA ATTCACCAAC GCCGGCACGT 
GCGGCCGGCC CACCACTGGT GTCTGACCCA TGGGACAATG ATCTGATCTC AGATTTGCTG 
TCGGCACTCA CTCCGCCGCT ACCCTCTCAC CCTCATTACA TCACCTGGTC CTGCAACATG 
CCCGCCATCA GCCCGCGGAT GACCATCACC ATGGGTAAAG CATCTCTAAG AGTCGACTGC 
ATCCTTGGAG AAGGAGCCTT CGCCACCGTG TATCAGGCCA CCAACCCTGC GACCTTAGAG 
AAGGTGGTGT TGAAGGTCCA GAAACCGGCC AATCCCTGGG AGTTTTACAT CAACACCCAG 
CTGGACGCTC GGCTGCAGCC CCACACACGT CACCTCTTCA GCAACATCCG TTCAGCTCAC 
CTGTTCAATA ACGGCAGCGT TCTGCTTGCT CAGCTCCACA ATTACGGCAC CTTGCTGAAT 
GCAGTGAACA TCTACAAAAC CCTGAGTGAG AAGCTGATGC CGCAGCCGCT TGTTATGTAC 
CTGGCTACCT GCATCCTGAC CATGGTGGAG CAGCTGCACG CCATCCACAT CATCCACGCT 
GACATCAAAC CTGACAACTT CCTACTGGGA GAGAGGTTCC TGGAGAACAG GTGCTTTGAG 
CCAGAGAACT TGGACCACGG CCTCGTCCTC ATTGACCTCG GCCAGAGCAT CGACCTGGAT 
CTTTTCCCAG AGGGCACCGC GTTCACTGCC AAGTGCCTGA CATCAGGCTT CCAGTGCACT 
GAGATGCTGA GTGGGAAACC ATGGAATTAC CAGTCGGATT ACTTTGGAGT TGCAGGGACG 
GTTCACTGCA TGCTGTTTGG GACCTACATG CAGGTGACAA ATGATGGCGG CGTGTGGAAG 
ACCAACGGTG TCTTCAGAAG AATTCCTCAC AGCGACCTGT GGCAGGAGTT CTTCCACGTT 
CTTCTGAACG TTCCGGACTG TTCCGGGCTG CCAAGCCTGC GGGGCCTGCG TGGGAAGCTG 
ACGTCGGTCC TGCAGCAGGA CTACAGCAAC AAACTCGGCA CGTTGAAGAG TCGTCTGGTG 
GTTCAGCTGC TGGAGCGCCG GAAAGCAGCC GCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610094.1
CDS	145..3723
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNMSQGGTGNLSHITPNTSLGFVQATPSRVLPSPTVNTREALDVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEGTTENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611792.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611792.2

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGAAAGCAG AAACCCTTTA 
CAAAATTCTC ATGTAACCAA TCAGCTGATG TCTCACCAAG AGCAACATAA CAAGGCCTCT 
GGGGACTGTT CTTTCAAACA TCCCACCATT GAAACCATTG TCACAGTTTC CCGCTCTGAG 
ACTTCTGGGA CCATTAACTC CAGCCAGAGC TCCAACGTTC AGTTTGTGTC CGAGTACCTG 
AAGGATGAGC TGCTCTGTGA GGGGTCCGAG TTGTGTTTCG AGGAGGTCCG AGCGGCGAAA 
TACTTCCGTA AACTGGAGGA GCAACAGGAT GATCAGACAG TAGATGTTTT GTTTGCAGAA 
GAGCAGTTTA TGAGGGTGGA GGAGGAGAAC TTCATGAGCA TGAGAGATGC ACTGGGGACA 
ATCAAGCAGG CTCTGGGGGG TGTAAAGAGC CACATGGGGC AGACAGCTGT AGTGGAAACA 
TCTGCTGCCA TGAACATTAA TCCTACTCAG CATTCCACCG GGCCTCCTCA GTTATCCAGT 
CGGCGCAGCA GCCGGCGCTC GCTCGGTTTG AGGCTGCTCA CTGAGCCCAC CTTCATCCAC 
GAGGCCCTTG CTGTCGTCCC TGACCAATAT CAGGAGGAGA GAAAGGTCAC ATCCTCTGAC 
CCTGATGAGA GACTGTGTCA TCCAGCTCTA ACTGACAGGA GTGTCCGGTC ACCCACATCT 
GCACCGACGT CTGCATCTAC GGAACCGTCA CCTGTTCTTC AGGCAGCTGC GGCAGAACAG 
ATGGCTCCGT CACTGCGGGG GGTCTCATCT ACTGAGAAAG TTCTCCTTCA GGAGGACCCA 
GAGATCAACA CTGCTGCAGC CCAGGATGTG GCATGTCTCC CTGAACCCGA CGACAGACTC 
AACATGTCAC AGGGTGGGAC CGGTAACCTT TCTCACATTA CTCCCAACAC ATCACTGGGT 
TTTGTTCAGG CCACACCATC ACGGGTGCTG CCGTCTCCCA CAGTCAACAC ACGGGAAGCT 
CTGGATGTTA TCATGGACAT GTTCCAGGCG CCAACCCTCC TCGAGGACCC GTTCAACAAA 
ACATCGGCTT TTCCCTCGTC AGAGGTGGAG GCAGAACCGG CCCCACTTAA ACATGGAGGG 
GACTCGTATT TCACTAAGCC CCGCGCTGCT GCCTCGTTTA CTATCTTCCA GGATGAACAA 
GACAAAGAAA ACATCAGTGC CGCTGCTCCT CATCCTTCAC TCAGCAAATC TAAACCAATC 
AAAGCTCTGG CTGAGATACG GCAGGTTAAA AAATCAAACG AGACGCCTCC TGAGCTGATG 
CCGGACGAGA GCACCATGTG GGGGGCCCGC TACAACTCCC TCAACTCCCT GGCGGCGTGT 
CCCAACAGCA CCACCGACTT CGCCATGTTG GCCCAGTGTG TGTCCACACC GTTCGCATTC 
AAGACGCCTC ACAGCAGCAA CTTCTTCCAG GATGAAGGTA CAACAGAGAA CAGCGGTGGT 
GGTGACGGTG ATGACGACGA ATTTATGAGA CGTCAACCGA AAAAACTCAG CCCCATCATG 
GAGCAGAGTC CAGCTGAAGA AACTGCCATC AGTCACATGG CCCTGCCCTC TGACAGGCTC 
GGGACCATTG TAGGTGAGGG TCTTGCCTCA GCCCAGCTCT GCTCGACCTC CTCCACCACC 
CTGGTGCAGG CTCCGCCCCC TGGTGTGTTG TCCTTCAGAG ACCAGACCAT CTCCACCAGG 
ACTACATTGG ACACAGACCA AGAGCAGCCC CCCAAGTCCA AGAGTCCATT TTCAATCAAG 
AGAGAACCAT TCAAGATACT GGAGGATCTG GATGAACCGG CCCAGAATCC AGTCGGTGAT 
GTTCCCATGA GTCCAGAGGG GACCCTCAAA CCTGACTGGC TGAGCATCGG GAGCCCTGAG 
GTTCCACCGG AAACTGACCT GGATGCTTTC CTGAGCCCAT GTCGGCCAAA GCGCGCTGAT 
GTGCCCATGA CCCCAGAACC ACCGCTCTGC ACAGCTGTAC CCATGAGCCC AGCAACGGCG 
CCGCAGTCAG ACGGAGGCCG GTGCTTGGAC GTGTCCATGA ATTCACCAAC GCCGGCACGT 
GCGGCCGGCC CACCACTGGT GTCTGACCCA TGGGACAATG ATCTGATCTC AGATTTGCTG 
TCGGCACTCA CTCCGCCGCT ACCCTCTCAC CCTCATTACA TCACCTGGTC CTGCAACATG 
CCCGCCATCA GCCCGCGGAT GACCATCACC ATGGGTAAAG CATCTCTAAG AGTCGACTGC 
ATCCTTGGAG AAGGAGCCTT CGCCACCGTG TATCAGGCCA CCAACCCTGC GACCTTAGAG 
AAGGTGGTGT TGAAGGTCCA GAAACCGGCC AATCCCTGGG AGTTTTACAT CAACACCCAG 
CTGGACGCTC GGCTGCAGCC CCACACACGT CACCTCTTCA GCAACATCCG TTCAGCTCAC 
CTGTTCAATA ACGGCAGCGT TCTGCTTGCT CAGCTCCACA ATTACGGCAC CTTGCTGAAT 
GCAGTGAACA TCTACAAAAC CCTGAGTGAG AAGCTGATGC CGCAGCCGCT TGTTATGTAC 
CTGGCTACCT GCATCCTGAC CATGGTGGAG CAGCTGCACG CCATCCACAT CATCCACGCT 
GACATCAAAC CTGACAACTT CCTACTGGGA GAGAGGTTCC TGGAGAACAG GTGCTTTGAG 
CCAGAGAACT TGGACCACGG CCTCGTCCTC ATTGACCTCG GCCAGAGCAT CGACCTGGAT 
CTTTTCCCAG AGGGCACCGC GTTCACTGCC AAGTGCCTGA CATCAGGCTT CCAGTGCACT 
GAGATGCTGA GTGGGAAACC ATGGAATTAC CAGTCGGATT ACTTTGGAGT TGCAGGGACG 
GTTCACTGCA TGCTGTTTGG GACCTACATG CAGGTGACAA ATGATGGCGG CGTGTGGAAG 
ACCAACGGTG TCTTCAGAAG AATTCCTCAC AGCGACCTGT GGCAGGAGTT CTTCCACGTT 
CTTCTGAACG TTCCGGACTG TTCCGGGCTG CCAAGCCTGC GGGGCCTGCG TGGGAAGCTG 
ACGTCGGTCC TGCAGCAGGA CTACAGCAAC AAACTCGGCA CGTTGAAGAG TCGTCTGGTG 
GTTCAGCTGC TGGAGCGCCG GAAAGCAGCC GCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_011611793.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610095.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

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CloneID	OTo51935
Clone ID Related Accession (Same CDS sequence)	XM_011611793.2
Accession Version	XM_011611793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042300.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011611793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACAATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CCACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCGGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG AGAACAGCGG TGGTGGTGAC 
GGTGATGACG ACGAATTTAT GAGACGTCAA CCGAAAAAAC TCAGCCCCAT CATGGAGCAG 
AGTCCAGCTG AAGAAACTGC CATCAGTCAC ATGGCCCTGC CCTCTGACAG GCTCGGGACC 
ATTGTAGGTG AGGGTCTTGC CTCAGCCCAG CTCTGCTCGA CCTCCTCCAC CACCCTGGTG 
CAGGCTCCGC CCCCTGGTGT GTTGTCCTTC AGAGACCAGA CCATCTCCAC CAGGACTACA 
TTGGACACAG ACCAAGAGCA GCCCCCCAAG TCCAAGAGTC CATTTTCAAT CAAGAGAGAA 
CCATTCAAGA TACTGGAGGA TCTGGATGAA CCGGCCCAGA ATCCAGTCGG TGATGTTCCC 
ATGAGTCCAG AGGGGACCCT CAAACCTGAC TGGCTGAGCA TCGGGAGCCC TGAGGTTCCA 
CCGGAAACTG ACCTGGATGC TTTCCTGAGC CCATGTCGGC CAAAGCGCGC TGATGTGCCC 
ATGACCCCAG AACCACCGCT CTGCACAGCT GTACCCATGA GCCCAGCAAC GGCGCCGCAG 
TCAGACGGAG GCCGGTGCTT GGACGTGTCC ATGAATTCAC CAACGCCGGC ACGTGCGGCC 
GGCCCACCAC TGGTGTCTGA CCCATGGGAC AATGATCTGA TCTCAGATTT GCTGTCGGCA 
CTCACTCCGC CGCTACCCTC TCACCCTCAT TACATCACCT GGTCCTGCAA CATGCCCGCC 
ATCAGCCCGC GGATGACCAT CACCATGGGT AAAGCATCTC TAAGAGTCGA CTGCATCCTT 
GGAGAAGGAG CCTTCGCCAC CGTGTATCAG GCCACCAACC CTGCGACCTT AGAGAAGGTG 
GTGTTGAAGG TCCAGAAACC GGCCAATCCC TGGGAGTTTT ACATCAACAC CCAGCTGGAC 
GCTCGGCTGC AGCCCCACAC ACGTCACCTC TTCAGCAACA TCCGTTCAGC TCACCTGTTC 
AATAACGGCA GCGTTCTGCT TGCTCAGCTC CACAATTACG GCACCTTGCT GAATGCAGTG 
AACATCTACA AAACCCTGAG TGAGAAGCTG ATGCCGCAGC CGCTTGTTAT GTACCTGGCT 
ACCTGCATCC TGACCATGGT GGAGCAGCTG CACGCCATCC ACATCATCCA CGCTGACATC 
AAACCTGACA ACTTCCTACT GGGAGAGAGG TTCCTGGAGA ACAGGTGCTT TGAGCCAGAG 
AACTTGGACC ACGGCCTCGT CCTCATTGAC CTCGGCCAGA GCATCGACCT GGATCTTTTC 
CCAGAGGGCA CCGCGTTCAC TGCCAAGTGC CTGACATCAG GCTTCCAGTG CACTGAGATG 
CTGAGTGGGA AACCATGGAA TTACCAGTCG GATTACTTTG GAGTTGCAGG GACGGTTCAC 
TGCATGCTGT TTGGGACCTA CATGCAGGTG ACAAATGATG GCGGCGTGTG GAAGACCAAC 
GGTGTCTTCA GAAGAATTCC TCACAGCGAC CTGTGGCAGG AGTTCTTCCA CGTTCTTCTG 
AACGTTCCGG ACTGTTCCGG GCTGCCAAGC CTGCGGGGCC TGCGTGGGAA GCTGACGTCG 
GTCCTGCAGC AGGACTACAG CAACAAACTC GGCACGTTGA AGAGTCGTCT GGTGGTTCAG 
CTGCTGGAGC GCCGGAAAGC AGCCGCAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610095.1
CDS	145..3717
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISAESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNMSQGGTGNLSHITPNTSLGFVQATPSRVLPSPTVNTREALDVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611793.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611793.2

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACAATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CCACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCGGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG AGAACAGCGG TGGTGGTGAC 
GGTGATGACG ACGAATTTAT GAGACGTCAA CCGAAAAAAC TCAGCCCCAT CATGGAGCAG 
AGTCCAGCTG AAGAAACTGC CATCAGTCAC ATGGCCCTGC CCTCTGACAG GCTCGGGACC 
ATTGTAGGTG AGGGTCTTGC CTCAGCCCAG CTCTGCTCGA CCTCCTCCAC CACCCTGGTG 
CAGGCTCCGC CCCCTGGTGT GTTGTCCTTC AGAGACCAGA CCATCTCCAC CAGGACTACA 
TTGGACACAG ACCAAGAGCA GCCCCCCAAG TCCAAGAGTC CATTTTCAAT CAAGAGAGAA 
CCATTCAAGA TACTGGAGGA TCTGGATGAA CCGGCCCAGA ATCCAGTCGG TGATGTTCCC 
ATGAGTCCAG AGGGGACCCT CAAACCTGAC TGGCTGAGCA TCGGGAGCCC TGAGGTTCCA 
CCGGAAACTG ACCTGGATGC TTTCCTGAGC CCATGTCGGC CAAAGCGCGC TGATGTGCCC 
ATGACCCCAG AACCACCGCT CTGCACAGCT GTACCCATGA GCCCAGCAAC GGCGCCGCAG 
TCAGACGGAG GCCGGTGCTT GGACGTGTCC ATGAATTCAC CAACGCCGGC ACGTGCGGCC 
GGCCCACCAC TGGTGTCTGA CCCATGGGAC AATGATCTGA TCTCAGATTT GCTGTCGGCA 
CTCACTCCGC CGCTACCCTC TCACCCTCAT TACATCACCT GGTCCTGCAA CATGCCCGCC 
ATCAGCCCGC GGATGACCAT CACCATGGGT AAAGCATCTC TAAGAGTCGA CTGCATCCTT 
GGAGAAGGAG CCTTCGCCAC CGTGTATCAG GCCACCAACC CTGCGACCTT AGAGAAGGTG 
GTGTTGAAGG TCCAGAAACC GGCCAATCCC TGGGAGTTTT ACATCAACAC CCAGCTGGAC 
GCTCGGCTGC AGCCCCACAC ACGTCACCTC TTCAGCAACA TCCGTTCAGC TCACCTGTTC 
AATAACGGCA GCGTTCTGCT TGCTCAGCTC CACAATTACG GCACCTTGCT GAATGCAGTG 
AACATCTACA AAACCCTGAG TGAGAAGCTG ATGCCGCAGC CGCTTGTTAT GTACCTGGCT 
ACCTGCATCC TGACCATGGT GGAGCAGCTG CACGCCATCC ACATCATCCA CGCTGACATC 
AAACCTGACA ACTTCCTACT GGGAGAGAGG TTCCTGGAGA ACAGGTGCTT TGAGCCAGAG 
AACTTGGACC ACGGCCTCGT CCTCATTGAC CTCGGCCAGA GCATCGACCT GGATCTTTTC 
CCAGAGGGCA CCGCGTTCAC TGCCAAGTGC CTGACATCAG GCTTCCAGTG CACTGAGATG 
CTGAGTGGGA AACCATGGAA TTACCAGTCG GATTACTTTG GAGTTGCAGG GACGGTTCAC 
TGCATGCTGT TTGGGACCTA CATGCAGGTG ACAAATGATG GCGGCGTGTG GAAGACCAAC 
GGTGTCTTCA GAAGAATTCC TCACAGCGAC CTGTGGCAGG AGTTCTTCCA CGTTCTTCTG 
AACGTTCCGG ACTGTTCCGG GCTGCCAAGC CTGCGGGGCC TGCGTGGGAA GCTGACGTCG 
GTCCTGCAGC AGGACTACAG CAACAAACTC GGCACGTTGA AGAGTCGTCT GGTGGTTCAG 
CTGCTGGAGC GCCGGAAAGC AGCCGCAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_011611794.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610096.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo51936
Clone ID Related Accession (Same CDS sequence)	XM_011611794.2
Accession Version	XM_011611794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042300.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011611794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACAATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACAATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCGGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTGAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTG GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610096.1
CDS	145..3606
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISAESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNNVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEGTTENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611794.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611794.2

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACAAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACAATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACAATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCGGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTGAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTG GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_011611791.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610093.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo30324
Clone ID Related Accession (Same CDS sequence)	XM_011611791.1
Accession Version	XM_011611791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACGATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CTACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCAGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTCAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTA GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610093.1
CDS	145..3726
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISAESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNMSQGGTGNLSHITPNTSLGFVQATPSRVLPSPTVNTREALDVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEGTTENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611791.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611791.1

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACGATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CTACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCAGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTCAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTA GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_011611792.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610094.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo30325
Clone ID Related Accession (Same CDS sequence)	XM_011611792.1
Accession Version	XM_011611792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGAAAGCAG AAACCCTTTA 
CAAAATTCTC ATGTAACCAA TCAGCTGATG TCTCACCAAG AGCAACATAA CAAGGCCTCT 
GGGGACTGTT CTTTCAAACA TCCCACCATT GAAACCATTG TCACAGTTTC CCGCTCTGAG 
ACTTCTGGGA CCATTAACTC CAGCCAGAGC TCCAACGTTC AGTTTGTGTC CGAGTACCTG 
AAGGATGAGC TGCTCTGTGA GGGGTCCGAG TTGTGTTTCG AGGAGGTCCG AGCGGCGAAA 
TACTTCCGTA AACTGGAGGA GCAACAGGAT GATCAGACAG TAGATGTTTT GTTTGCAGAA 
GAGCAGTTTA TGAGGGTGGA GGAGGAGAAC TTCATGAGCA TGAGAGATGC ACTGGGGACG 
ATCAAGCAGG CTCTGGGGGG TGTAAAGAGC CACATGGGGC AGACAGCTGT AGTGGAAACA 
TCTGCTGCCA TGAACATTAA TCCTACTCAG CATTCCACCG GGCCTCCTCA GTTATCCAGT 
CGGCGCAGCA GCCGGCGCTC GCTCGGTTTG AGGCTGCTCA CTGAGCCCAC CTTCATCCAC 
GAGGCCCTTG CTGTCGTCCC TGACCAATAT CAGGAGGAGA GAAAGGTCAC ATCCTCTGAC 
CCTGATGAGA GACTGTGTCA TCCAGCTCTA ACTGACAGGA GTGTCCGGTC ACCCACATCT 
GCACCGACGT CTGCATCTAC GGAACCGTCA CCTGTTCTTC AGGCAGCTGC GGCAGAACAG 
ATGGCTCCGT CACTGCGGGG GGTCTCATCT ACTGAGAAAG TTCTCCTTCA GGAGGACCCA 
GAGATCAACA CTGCTGCAGC CCAGGATGTG GCATGTCTCC CTGAACCCGA CGACAGACTC 
AACATGTCAC AGGGTGGGAC CGGTAACCTT TCTCACATTA CTCCCAACAC ATCACTGGGT 
TTTGTTCAGG CCACACCATC ACGGGTGCTG CCGTCTCCTA CAGTCAACAC ACGGGAAGCT 
CTGGATGTTA TCATGGACAT GTTCCAGGCG CCAACCCTCC TCGAGGACCC GTTCAACAAA 
ACATCGGCTT TTCCCTCGTC AGAGGTGGAG GCAGAACCGG CCCCACTTAA ACATGGAGGG 
GACTCGTATT TCACTAAGCC CCGCGCTGCT GCCTCGTTTA CTATCTTCCA GGATGAACAA 
GACAAAGAAA ACATCAGTGC CGCTGCTCCT CATCCTTCAC TCAGCAAATC TAAACCAATC 
AAAGCTCTGG CTGAGATACG GCAGGTTAAA AAATCAAACG AGACGCCTCC TGAGCTGATG 
CCGGACGAGA GCACCATGTG GGGGGCCCGC TACAACTCCC TCAACTCCCT GGCAGCGTGT 
CCCAACAGCA CCACCGACTT CGCCATGTTG GCCCAGTGTG TGTCCACACC GTTCGCATTC 
AAGACGCCTC ACAGCAGCAA CTTCTTCCAG GATGAAGGTA CAACAGAGAA CAGCGGTGGT 
GGTGACGGTG ATGACGACGA ATTTATGAGA CGTCAACCGA AAAAACTCAG CCCCATCATG 
GAGCAGAGTC CAGCTGAAGA AACTGCCATC AGTCACATGG CCCTGCCCTC TGACAGGCTC 
GGGACCATTG TAGGTGAGGG TCTTGCCTCA GCCCAGCTCT GCTCGACCTC CTCCACCACC 
CTGGTGCAGG CTCCGCCCCC TGGTGTGTTG TCCTTCAGAG ACCAGACCAT CTCCACCAGG 
ACTACATTGG ACACAGACCA AGAGCAGCCC CCCAAGTCCA AGAGTCCATT TTCAATCAAG 
AGAGAACCAT TCAAGATACT GGAGGATCTG GATGAACCGG CCCAGAATCC AGTCGGTGAT 
GTTCCCATGA GTCCAGAGGG GACCCTCAAA CCTGACTGGC TCAGCATCGG GAGCCCTGAG 
GTTCCACCGG AAACTGACCT GGATGCTTTC CTGAGCCCAT GTCGGCCAAA GCGCGCTGAT 
GTGCCCATGA CCCCAGAACC ACCGCTCTGC ACAGCTGTAC CCATGAGCCC AGCAACGGCG 
CCGCAGTCAG ACGGAGGCCG GTGCTTAGAC GTGTCCATGA ATTCACCAAC GCCGGCACGT 
GCGGCCGGCC CACCACTGGT GTCTGACCCA TGGGACAATG ATCTGATCTC AGATTTGCTG 
TCGGCACTCA CTCCGCCGCT ACCCTCTCAC CCTCATTACA TCACCTGGTC CTGCAACATG 
CCCGCCATCA GCCCGCGGAT GACCATCACC ATGGGTAAAG CATCTCTAAG AGTCGACTGC 
ATCCTTGGAG AAGGAGCCTT CGCCACCGTG TATCAGGCCA CCAACCCTGC GACCTTAGAG 
AAGGTGGTGT TGAAGGTCCA GAAACCGGCC AATCCCTGGG AGTTTTACAT CAACACCCAG 
CTGGACGCTC GGCTGCAGCC CCACACACGT CACCTCTTCA GCAACATCCG TTCAGCTCAC 
CTGTTCAATA ACGGCAGCGT TCTGCTTGCT CAGCTCCACA ATTACGGCAC CTTGCTGAAT 
GCAGTGAACA TCTACAAAAC CCTGAGTGAG AAGCTGATGC CGCAGCCGCT TGTTATGTAC 
CTGGCTACCT GCATCCTGAC CATGGTGGAG CAGCTGCACG CCATCCACAT CATCCACGCT 
GACATCAAAC CTGACAACTT CCTACTGGGA GAGAGGTTCC TGGAGAACAG GTGCTTTGAG 
CCAGAGAACT TGGACCACGG CCTCGTCCTC ATTGACCTCG GCCAGAGCAT CGACCTGGAT 
CTTTTCCCAG AGGGCACCGC GTTCACTGCC AAGTGCCTGA CATCAGGCTT CCAGTGCACT 
GAGATGCTGA GTGGGAAACC ATGGAATTAC CAGTCGGATT ACTTTGGAGT TGCAGGGACG 
GTTCACTGCA TGCTGTTTGG GACCTACATG CAGGTGACAA ATGATGGCGG CGTGTGGAAG 
ACCAACGGTG TCTTCAGAAG AATTCCTCAC AGCGACCTGT GGCAGGAGTT CTTCCACGTT 
CTTCTGAACG TTCCGGACTG TTCCGGGCTG CCAAGCCTGC GGGGCCTGCG TGGGAAGCTG 
ACGTCGGTCC TGCAGCAGGA CTACAGCAAC AAACTCGGCA CGTTGAAGAG TCGTCTGGTG 
GTTCAGCTGC TGGAGCGCCG GAAAGCAGCC GCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610094.1
CDS	145..3723
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNMSQGGTGNLSHITPNTSLGFVQATPSRVLPSPTVNTREALDVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEGTTENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611792.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611792.1

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGAAAGCAG AAACCCTTTA 
CAAAATTCTC ATGTAACCAA TCAGCTGATG TCTCACCAAG AGCAACATAA CAAGGCCTCT 
GGGGACTGTT CTTTCAAACA TCCCACCATT GAAACCATTG TCACAGTTTC CCGCTCTGAG 
ACTTCTGGGA CCATTAACTC CAGCCAGAGC TCCAACGTTC AGTTTGTGTC CGAGTACCTG 
AAGGATGAGC TGCTCTGTGA GGGGTCCGAG TTGTGTTTCG AGGAGGTCCG AGCGGCGAAA 
TACTTCCGTA AACTGGAGGA GCAACAGGAT GATCAGACAG TAGATGTTTT GTTTGCAGAA 
GAGCAGTTTA TGAGGGTGGA GGAGGAGAAC TTCATGAGCA TGAGAGATGC ACTGGGGACG 
ATCAAGCAGG CTCTGGGGGG TGTAAAGAGC CACATGGGGC AGACAGCTGT AGTGGAAACA 
TCTGCTGCCA TGAACATTAA TCCTACTCAG CATTCCACCG GGCCTCCTCA GTTATCCAGT 
CGGCGCAGCA GCCGGCGCTC GCTCGGTTTG AGGCTGCTCA CTGAGCCCAC CTTCATCCAC 
GAGGCCCTTG CTGTCGTCCC TGACCAATAT CAGGAGGAGA GAAAGGTCAC ATCCTCTGAC 
CCTGATGAGA GACTGTGTCA TCCAGCTCTA ACTGACAGGA GTGTCCGGTC ACCCACATCT 
GCACCGACGT CTGCATCTAC GGAACCGTCA CCTGTTCTTC AGGCAGCTGC GGCAGAACAG 
ATGGCTCCGT CACTGCGGGG GGTCTCATCT ACTGAGAAAG TTCTCCTTCA GGAGGACCCA 
GAGATCAACA CTGCTGCAGC CCAGGATGTG GCATGTCTCC CTGAACCCGA CGACAGACTC 
AACATGTCAC AGGGTGGGAC CGGTAACCTT TCTCACATTA CTCCCAACAC ATCACTGGGT 
TTTGTTCAGG CCACACCATC ACGGGTGCTG CCGTCTCCTA CAGTCAACAC ACGGGAAGCT 
CTGGATGTTA TCATGGACAT GTTCCAGGCG CCAACCCTCC TCGAGGACCC GTTCAACAAA 
ACATCGGCTT TTCCCTCGTC AGAGGTGGAG GCAGAACCGG CCCCACTTAA ACATGGAGGG 
GACTCGTATT TCACTAAGCC CCGCGCTGCT GCCTCGTTTA CTATCTTCCA GGATGAACAA 
GACAAAGAAA ACATCAGTGC CGCTGCTCCT CATCCTTCAC TCAGCAAATC TAAACCAATC 
AAAGCTCTGG CTGAGATACG GCAGGTTAAA AAATCAAACG AGACGCCTCC TGAGCTGATG 
CCGGACGAGA GCACCATGTG GGGGGCCCGC TACAACTCCC TCAACTCCCT GGCAGCGTGT 
CCCAACAGCA CCACCGACTT CGCCATGTTG GCCCAGTGTG TGTCCACACC GTTCGCATTC 
AAGACGCCTC ACAGCAGCAA CTTCTTCCAG GATGAAGGTA CAACAGAGAA CAGCGGTGGT 
GGTGACGGTG ATGACGACGA ATTTATGAGA CGTCAACCGA AAAAACTCAG CCCCATCATG 
GAGCAGAGTC CAGCTGAAGA AACTGCCATC AGTCACATGG CCCTGCCCTC TGACAGGCTC 
GGGACCATTG TAGGTGAGGG TCTTGCCTCA GCCCAGCTCT GCTCGACCTC CTCCACCACC 
CTGGTGCAGG CTCCGCCCCC TGGTGTGTTG TCCTTCAGAG ACCAGACCAT CTCCACCAGG 
ACTACATTGG ACACAGACCA AGAGCAGCCC CCCAAGTCCA AGAGTCCATT TTCAATCAAG 
AGAGAACCAT TCAAGATACT GGAGGATCTG GATGAACCGG CCCAGAATCC AGTCGGTGAT 
GTTCCCATGA GTCCAGAGGG GACCCTCAAA CCTGACTGGC TCAGCATCGG GAGCCCTGAG 
GTTCCACCGG AAACTGACCT GGATGCTTTC CTGAGCCCAT GTCGGCCAAA GCGCGCTGAT 
GTGCCCATGA CCCCAGAACC ACCGCTCTGC ACAGCTGTAC CCATGAGCCC AGCAACGGCG 
CCGCAGTCAG ACGGAGGCCG GTGCTTAGAC GTGTCCATGA ATTCACCAAC GCCGGCACGT 
GCGGCCGGCC CACCACTGGT GTCTGACCCA TGGGACAATG ATCTGATCTC AGATTTGCTG 
TCGGCACTCA CTCCGCCGCT ACCCTCTCAC CCTCATTACA TCACCTGGTC CTGCAACATG 
CCCGCCATCA GCCCGCGGAT GACCATCACC ATGGGTAAAG CATCTCTAAG AGTCGACTGC 
ATCCTTGGAG AAGGAGCCTT CGCCACCGTG TATCAGGCCA CCAACCCTGC GACCTTAGAG 
AAGGTGGTGT TGAAGGTCCA GAAACCGGCC AATCCCTGGG AGTTTTACAT CAACACCCAG 
CTGGACGCTC GGCTGCAGCC CCACACACGT CACCTCTTCA GCAACATCCG TTCAGCTCAC 
CTGTTCAATA ACGGCAGCGT TCTGCTTGCT CAGCTCCACA ATTACGGCAC CTTGCTGAAT 
GCAGTGAACA TCTACAAAAC CCTGAGTGAG AAGCTGATGC CGCAGCCGCT TGTTATGTAC 
CTGGCTACCT GCATCCTGAC CATGGTGGAG CAGCTGCACG CCATCCACAT CATCCACGCT 
GACATCAAAC CTGACAACTT CCTACTGGGA GAGAGGTTCC TGGAGAACAG GTGCTTTGAG 
CCAGAGAACT TGGACCACGG CCTCGTCCTC ATTGACCTCG GCCAGAGCAT CGACCTGGAT 
CTTTTCCCAG AGGGCACCGC GTTCACTGCC AAGTGCCTGA CATCAGGCTT CCAGTGCACT 
GAGATGCTGA GTGGGAAACC ATGGAATTAC CAGTCGGATT ACTTTGGAGT TGCAGGGACG 
GTTCACTGCA TGCTGTTTGG GACCTACATG CAGGTGACAA ATGATGGCGG CGTGTGGAAG 
ACCAACGGTG TCTTCAGAAG AATTCCTCAC AGCGACCTGT GGCAGGAGTT CTTCCACGTT 
CTTCTGAACG TTCCGGACTG TTCCGGGCTG CCAAGCCTGC GGGGCCTGCG TGGGAAGCTG 
ACGTCGGTCC TGCAGCAGGA CTACAGCAAC AAACTCGGCA CGTTGAAGAG TCGTCTGGTG 
GTTCAGCTGC TGGAGCGCCG GAAAGCAGCC GCAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_011611793.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610095.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo30326
Clone ID Related Accession (Same CDS sequence)	XM_011611793.1
Accession Version	XM_011611793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACGATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CTACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCAGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG AGAACAGCGG TGGTGGTGAC 
GGTGATGACG ACGAATTTAT GAGACGTCAA CCGAAAAAAC TCAGCCCCAT CATGGAGCAG 
AGTCCAGCTG AAGAAACTGC CATCAGTCAC ATGGCCCTGC CCTCTGACAG GCTCGGGACC 
ATTGTAGGTG AGGGTCTTGC CTCAGCCCAG CTCTGCTCGA CCTCCTCCAC CACCCTGGTG 
CAGGCTCCGC CCCCTGGTGT GTTGTCCTTC AGAGACCAGA CCATCTCCAC CAGGACTACA 
TTGGACACAG ACCAAGAGCA GCCCCCCAAG TCCAAGAGTC CATTTTCAAT CAAGAGAGAA 
CCATTCAAGA TACTGGAGGA TCTGGATGAA CCGGCCCAGA ATCCAGTCGG TGATGTTCCC 
ATGAGTCCAG AGGGGACCCT CAAACCTGAC TGGCTCAGCA TCGGGAGCCC TGAGGTTCCA 
CCGGAAACTG ACCTGGATGC TTTCCTGAGC CCATGTCGGC CAAAGCGCGC TGATGTGCCC 
ATGACCCCAG AACCACCGCT CTGCACAGCT GTACCCATGA GCCCAGCAAC GGCGCCGCAG 
TCAGACGGAG GCCGGTGCTT AGACGTGTCC ATGAATTCAC CAACGCCGGC ACGTGCGGCC 
GGCCCACCAC TGGTGTCTGA CCCATGGGAC AATGATCTGA TCTCAGATTT GCTGTCGGCA 
CTCACTCCGC CGCTACCCTC TCACCCTCAT TACATCACCT GGTCCTGCAA CATGCCCGCC 
ATCAGCCCGC GGATGACCAT CACCATGGGT AAAGCATCTC TAAGAGTCGA CTGCATCCTT 
GGAGAAGGAG CCTTCGCCAC CGTGTATCAG GCCACCAACC CTGCGACCTT AGAGAAGGTG 
GTGTTGAAGG TCCAGAAACC GGCCAATCCC TGGGAGTTTT ACATCAACAC CCAGCTGGAC 
GCTCGGCTGC AGCCCCACAC ACGTCACCTC TTCAGCAACA TCCGTTCAGC TCACCTGTTC 
AATAACGGCA GCGTTCTGCT TGCTCAGCTC CACAATTACG GCACCTTGCT GAATGCAGTG 
AACATCTACA AAACCCTGAG TGAGAAGCTG ATGCCGCAGC CGCTTGTTAT GTACCTGGCT 
ACCTGCATCC TGACCATGGT GGAGCAGCTG CACGCCATCC ACATCATCCA CGCTGACATC 
AAACCTGACA ACTTCCTACT GGGAGAGAGG TTCCTGGAGA ACAGGTGCTT TGAGCCAGAG 
AACTTGGACC ACGGCCTCGT CCTCATTGAC CTCGGCCAGA GCATCGACCT GGATCTTTTC 
CCAGAGGGCA CCGCGTTCAC TGCCAAGTGC CTGACATCAG GCTTCCAGTG CACTGAGATG 
CTGAGTGGGA AACCATGGAA TTACCAGTCG GATTACTTTG GAGTTGCAGG GACGGTTCAC 
TGCATGCTGT TTGGGACCTA CATGCAGGTG ACAAATGATG GCGGCGTGTG GAAGACCAAC 
GGTGTCTTCA GAAGAATTCC TCACAGCGAC CTGTGGCAGG AGTTCTTCCA CGTTCTTCTG 
AACGTTCCGG ACTGTTCCGG GCTGCCAAGC CTGCGGGGCC TGCGTGGGAA GCTGACGTCG 
GTCCTGCAGC AGGACTACAG CAACAAACTC GGCACGTTGA AGAGTCGTCT GGTGGTTCAG 
CTGCTGGAGC GCCGGAAAGC AGCCGCAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610095.1
CDS	145..3717
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISAESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNMSQGGTGNLSHITPNTSLGFVQATPSRVLPSPTVNTREALDVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611793.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611793.1

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACGATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACATGT CACAGGGTGG GACCGGTAAC CTTTCTCACA TTACTCCCAA CACATCACTG 
GGTTTTGTTC AGGCCACACC ATCACGGGTG CTGCCGTCTC CTACAGTCAA CACACGGGAA 
GCTCTGGATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCAGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG AGAACAGCGG TGGTGGTGAC 
GGTGATGACG ACGAATTTAT GAGACGTCAA CCGAAAAAAC TCAGCCCCAT CATGGAGCAG 
AGTCCAGCTG AAGAAACTGC CATCAGTCAC ATGGCCCTGC CCTCTGACAG GCTCGGGACC 
ATTGTAGGTG AGGGTCTTGC CTCAGCCCAG CTCTGCTCGA CCTCCTCCAC CACCCTGGTG 
CAGGCTCCGC CCCCTGGTGT GTTGTCCTTC AGAGACCAGA CCATCTCCAC CAGGACTACA 
TTGGACACAG ACCAAGAGCA GCCCCCCAAG TCCAAGAGTC CATTTTCAAT CAAGAGAGAA 
CCATTCAAGA TACTGGAGGA TCTGGATGAA CCGGCCCAGA ATCCAGTCGG TGATGTTCCC 
ATGAGTCCAG AGGGGACCCT CAAACCTGAC TGGCTCAGCA TCGGGAGCCC TGAGGTTCCA 
CCGGAAACTG ACCTGGATGC TTTCCTGAGC CCATGTCGGC CAAAGCGCGC TGATGTGCCC 
ATGACCCCAG AACCACCGCT CTGCACAGCT GTACCCATGA GCCCAGCAAC GGCGCCGCAG 
TCAGACGGAG GCCGGTGCTT AGACGTGTCC ATGAATTCAC CAACGCCGGC ACGTGCGGCC 
GGCCCACCAC TGGTGTCTGA CCCATGGGAC AATGATCTGA TCTCAGATTT GCTGTCGGCA 
CTCACTCCGC CGCTACCCTC TCACCCTCAT TACATCACCT GGTCCTGCAA CATGCCCGCC 
ATCAGCCCGC GGATGACCAT CACCATGGGT AAAGCATCTC TAAGAGTCGA CTGCATCCTT 
GGAGAAGGAG CCTTCGCCAC CGTGTATCAG GCCACCAACC CTGCGACCTT AGAGAAGGTG 
GTGTTGAAGG TCCAGAAACC GGCCAATCCC TGGGAGTTTT ACATCAACAC CCAGCTGGAC 
GCTCGGCTGC AGCCCCACAC ACGTCACCTC TTCAGCAACA TCCGTTCAGC TCACCTGTTC 
AATAACGGCA GCGTTCTGCT TGCTCAGCTC CACAATTACG GCACCTTGCT GAATGCAGTG 
AACATCTACA AAACCCTGAG TGAGAAGCTG ATGCCGCAGC CGCTTGTTAT GTACCTGGCT 
ACCTGCATCC TGACCATGGT GGAGCAGCTG CACGCCATCC ACATCATCCA CGCTGACATC 
AAACCTGACA ACTTCCTACT GGGAGAGAGG TTCCTGGAGA ACAGGTGCTT TGAGCCAGAG 
AACTTGGACC ACGGCCTCGT CCTCATTGAC CTCGGCCAGA GCATCGACCT GGATCTTTTC 
CCAGAGGGCA CCGCGTTCAC TGCCAAGTGC CTGACATCAG GCTTCCAGTG CACTGAGATG 
CTGAGTGGGA AACCATGGAA TTACCAGTCG GATTACTTTG GAGTTGCAGG GACGGTTCAC 
TGCATGCTGT TTGGGACCTA CATGCAGGTG ACAAATGATG GCGGCGTGTG GAAGACCAAC 
GGTGTCTTCA GAAGAATTCC TCACAGCGAC CTGTGGCAGG AGTTCTTCCA CGTTCTTCTG 
AACGTTCCGG ACTGTTCCGG GCTGCCAAGC CTGCGGGGCC TGCGTGGGAA GCTGACGTCG 
GTCCTGCAGC AGGACTACAG CAACAAACTC GGCACGTTGA AGAGTCGTCT GGTGGTTCAG 
CTGCTGGAGC GCCGGAAAGC AGCCGCAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_011611794.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011610096.1 Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

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CloneID	OTo30327
Clone ID Related Accession (Same CDS sequence)	XM_011611794.1
Accession Version	XM_011611794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACGATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACAATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCAGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTCAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTA GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610096.1
CDS	145..3606
Translation	MDINAWVECFEKRLSTYTGDDPLDQWDKFVDYLEQRIPEDDRREMSQVFDSLVQRFLTVERYSDDIRYVKYCIKCASFYQDPTALYTHVYSKGVGHRTAALYVTWAQHFEHRGMYEEADAVYQKAVKNQAQPAETVLNEYRQFQTRTKPKTSISAESRNPLQNSHVTNQLMSHQEQHNKASGDCSFKHPTIETIVTVSRSETSGTINSSQSSNVQFVSEYLKDELLCEGSELCFEEVRAAKYFRKLEEQQDDQTVDVLFAEEQFMRVEEENFMSMRDALGTIKQALGGVKSHMGQTAVVETSAAMNINPTQHSTGPPQLSSRRSSRRSLGLRLLTEPTFIHEALAVVPDQYQEERKVTSSDPDERLCHPALTDRSVRSPTSAPTSASTEPSPVLQAAAAEQMAPSLRGVSSTEKVLLQEDPEINTAAAQDVACLPEPDDRLNNVIMDMFQAPTLLEDPFNKTSAFPSSEVEAEPAPLKHGGDSYFTKPRAAASFTIFQDEQDKENISAAAPHPSLSKSKPIKALAEIRQVKKSNETPPELMPDESTMWGARYNSLNSLAACPNSTTDFAMLAQCVSTPFAFKTPHSSNFFQDEGTTENSGGGDGDDDEFMRRQPKKLSPIMEQSPAEETAISHMALPSDRLGTIVGEGLASAQLCSTSSTTLVQAPPPGVLSFRDQTISTRTTLDTDQEQPPKSKSPFSIKREPFKILEDLDEPAQNPVGDVPMSPEGTLKPDWLSIGSPEVPPETDLDAFLSPCRPKRADVPMTPEPPLCTAVPMSPATAPQSDGGRCLDVSMNSPTPARAAGPPLVSDPWDNDLISDLLSALTPPLPSHPHYITWSCNMPAISPRMTITMGKASLRVDCILGEGAFATVYQATNPATLEKVVLKVQKPANPWEFYINTQLDARLQPHTRHLFSNIRSAHLFNNGSVLLAQLHNYGTLLNAVNIYKTLSEKLMPQPLVMYLATCILTMVEQLHAIHIIHADIKPDNFLLGERFLENRCFEPENLDHGLVLIDLGQSIDLDLFPEGTAFTAKCLTSGFQCTEMLSGKPWNYQSDYFGVAGTVHCMLFGTYMQVTNDGGVWKTNGVFRRIPHSDLWQEFFHVLLNVPDCSGLPSLRGLRGKLTSVLQQDYSNKLGTLKSRLVVQLLERRKAAAK

Target ORF information:

RefSeq Version	XM_011611794.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611794.1

ORF Insert Sequence:

ATGGATATCA ACGCCTGGGT CGAATGTTTT GAAAAAAGGT TGAGCACGTA TACGGGAGAT 
GATCCACTGG ATCAATGGGA TAAGTTTGTA GATTATCTTG AGCAGAGGAT ACCTGAGGAC 
GACCGTCGTG AGATGTCTCA GGTGTTTGAC AGCCTTGTGC AGAGGTTTTT GACTGTTGAG 
AGATACTCGG ACGACATCAG ATATGTGAAG TACTGCATCA AATGTGCGAG TTTTTACCAG 
GACCCCACTG CTCTGTACAC CCATGTTTAC AGTAAAGGGG TTGGCCACAG GACTGCTGCC 
CTCTACGTGA CCTGGGCACA GCACTTTGAA CACAGAGGGA TGTATGAAGA GGCAGATGCC 
GTCTACCAGA AAGCTGTGAA GAACCAAGCC CAGCCAGCTG AGACTGTTCT CAATGAATAC 
AGGCAGTTTC AAACGAGAAC CAAACCCAAG ACTTCAATCT CTGCAGAAAG CAGAAACCCT 
TTACAAAATT CTCATGTAAC CAATCAGCTG ATGTCTCACC AAGAGCAACA TAACAAGGCC 
TCTGGGGACT GTTCTTTCAA ACATCCCACC ATTGAAACCA TTGTCACAGT TTCCCGCTCT 
GAGACTTCTG GGACCATTAA CTCCAGCCAG AGCTCCAACG TTCAGTTTGT GTCCGAGTAC 
CTGAAGGATG AGCTGCTCTG TGAGGGGTCC GAGTTGTGTT TCGAGGAGGT CCGAGCGGCG 
AAATACTTCC GTAAACTGGA GGAGCAACAG GATGATCAGA CAGTAGATGT TTTGTTTGCA 
GAAGAGCAGT TTATGAGGGT GGAGGAGGAG AACTTCATGA GCATGAGAGA TGCACTGGGG 
ACGATCAAGC AGGCTCTGGG GGGTGTAAAG AGCCACATGG GGCAGACAGC TGTAGTGGAA 
ACATCTGCTG CCATGAACAT TAATCCTACT CAGCATTCCA CCGGGCCTCC TCAGTTATCC 
AGTCGGCGCA GCAGCCGGCG CTCGCTCGGT TTGAGGCTGC TCACTGAGCC CACCTTCATC 
CACGAGGCCC TTGCTGTCGT CCCTGACCAA TATCAGGAGG AGAGAAAGGT CACATCCTCT 
GACCCTGATG AGAGACTGTG TCATCCAGCT CTAACTGACA GGAGTGTCCG GTCACCCACA 
TCTGCACCGA CGTCTGCATC TACGGAACCG TCACCTGTTC TTCAGGCAGC TGCGGCAGAA 
CAGATGGCTC CGTCACTGCG GGGGGTCTCA TCTACTGAGA AAGTTCTCCT TCAGGAGGAC 
CCAGAGATCA ACACTGCTGC AGCCCAGGAT GTGGCATGTC TCCCTGAACC CGACGACAGA 
CTCAACAATG TTATCATGGA CATGTTCCAG GCGCCAACCC TCCTCGAGGA CCCGTTCAAC 
AAAACATCGG CTTTTCCCTC GTCAGAGGTG GAGGCAGAAC CGGCCCCACT TAAACATGGA 
GGGGACTCGT ATTTCACTAA GCCCCGCGCT GCTGCCTCGT TTACTATCTT CCAGGATGAA 
CAAGACAAAG AAAACATCAG TGCCGCTGCT CCTCATCCTT CACTCAGCAA ATCTAAACCA 
ATCAAAGCTC TGGCTGAGAT ACGGCAGGTT AAAAAATCAA ACGAGACGCC TCCTGAGCTG 
ATGCCGGACG AGAGCACCAT GTGGGGGGCC CGCTACAACT CCCTCAACTC CCTGGCAGCG 
TGTCCCAACA GCACCACCGA CTTCGCCATG TTGGCCCAGT GTGTGTCCAC ACCGTTCGCA 
TTCAAGACGC CTCACAGCAG CAACTTCTTC CAGGATGAAG GTACAACAGA GAACAGCGGT 
GGTGGTGACG GTGATGACGA CGAATTTATG AGACGTCAAC CGAAAAAACT CAGCCCCATC 
ATGGAGCAGA GTCCAGCTGA AGAAACTGCC ATCAGTCACA TGGCCCTGCC CTCTGACAGG 
CTCGGGACCA TTGTAGGTGA GGGTCTTGCC TCAGCCCAGC TCTGCTCGAC CTCCTCCACC 
ACCCTGGTGC AGGCTCCGCC CCCTGGTGTG TTGTCCTTCA GAGACCAGAC CATCTCCACC 
AGGACTACAT TGGACACAGA CCAAGAGCAG CCCCCCAAGT CCAAGAGTCC ATTTTCAATC 
AAGAGAGAAC CATTCAAGAT ACTGGAGGAT CTGGATGAAC CGGCCCAGAA TCCAGTCGGT 
GATGTTCCCA TGAGTCCAGA GGGGACCCTC AAACCTGACT GGCTCAGCAT CGGGAGCCCT 
GAGGTTCCAC CGGAAACTGA CCTGGATGCT TTCCTGAGCC CATGTCGGCC AAAGCGCGCT 
GATGTGCCCA TGACCCCAGA ACCACCGCTC TGCACAGCTG TACCCATGAG CCCAGCAACG 
GCGCCGCAGT CAGACGGAGG CCGGTGCTTA GACGTGTCCA TGAATTCACC AACGCCGGCA 
CGTGCGGCCG GCCCACCACT GGTGTCTGAC CCATGGGACA ATGATCTGAT CTCAGATTTG 
CTGTCGGCAC TCACTCCGCC GCTACCCTCT CACCCTCATT ACATCACCTG GTCCTGCAAC 
ATGCCCGCCA TCAGCCCGCG GATGACCATC ACCATGGGTA AAGCATCTCT AAGAGTCGAC 
TGCATCCTTG GAGAAGGAGC CTTCGCCACC GTGTATCAGG CCACCAACCC TGCGACCTTA 
GAGAAGGTGG TGTTGAAGGT CCAGAAACCG GCCAATCCCT GGGAGTTTTA CATCAACACC 
CAGCTGGACG CTCGGCTGCA GCCCCACACA CGTCACCTCT TCAGCAACAT CCGTTCAGCT 
CACCTGTTCA ATAACGGCAG CGTTCTGCTT GCTCAGCTCC ACAATTACGG CACCTTGCTG 
AATGCAGTGA ACATCTACAA AACCCTGAGT GAGAAGCTGA TGCCGCAGCC GCTTGTTATG 
TACCTGGCTA CCTGCATCCT GACCATGGTG GAGCAGCTGC ACGCCATCCA CATCATCCAC 
GCTGACATCA AACCTGACAA CTTCCTACTG GGAGAGAGGT TCCTGGAGAA CAGGTGCTTT 
GAGCCAGAGA ACTTGGACCA CGGCCTCGTC CTCATTGACC TCGGCCAGAG CATCGACCTG 
GATCTTTTCC CAGAGGGCAC CGCGTTCACT GCCAAGTGCC TGACATCAGG CTTCCAGTGC 
ACTGAGATGC TGAGTGGGAA ACCATGGAAT TACCAGTCGG ATTACTTTGG AGTTGCAGGG 
ACGGTTCACT GCATGCTGTT TGGGACCTAC ATGCAGGTGA CAAATGATGG CGGCGTGTGG 
AAGACCAACG GTGTCTTCAG AAGAATTCCT CACAGCGACC TGTGGCAGGA GTTCTTCCAC 
GTTCTTCTGA ACGTTCCGGA CTGTTCCGGG CTGCCAAGCC TGCGGGGCCT GCGTGGGAAG 
CTGACGTCGG TCCTGCAGCA GGACTACAGC AACAAACTCG GCACGTTGAA GAGTCGTCTG 
GTGGTTCAGC TGCTGGAGCG CCGGAAAGCA GCCGCAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

bub1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム