LOC101074204 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101074204
Entrez Gene ID	101074204
Full Name	adenylate cyclase type 9-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011612522.1	XP_011610824.1	adenylate cyclase type 9-like isoform X1
XM_011612524.1	XP_011610826.1	adenylate cyclase type 9-like isoform X3
XM_011612522.2	XP_011610824.1	adenylate cyclase type 9-like isoform X1
XM_011612523.1	XP_011610825.1	adenylate cyclase type 9-like isoform X2
XM_029850688.1	XP_029706548.1	adenylate cyclase type 9-like isoform X1
XM_011612524.2	XP_011610826.1	adenylate cyclase type 9-like isoform X3
XM_011612523.2	XP_011610825.1	adenylate cyclase type 9-like isoform X2
XM_029850689.1	XP_029706549.1	adenylate cyclase type 9-like isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC101074204 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101074204 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo52876	XM_029850688.1 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo52876	XM_011612522.2 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo52877	XM_011612523.2 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo52878	XM_011612524.2 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo52879	XM_029850689.1 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo28129	XM_011612522.1 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OTo28130	XM_011612523.1 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OTo28131	XM_011612524.1 Latest version!	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101074204 ( XM_011612522.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610824.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo52876
Clone ID Related Accession (Same CDS sequence)	XM_011612522.2 , XM_029850688.1
Accession Version	XM_011612522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	adenylate cyclase type 9-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGTAC AGGATGAACA CATTGTCGTC TGGTGGTTCA 
CCATCAGTCA CCCCTGGCCC TGACCCTCCT CCAGAACCTC TGGACATTCT AATCCCAGAA 
GCTGTTCTTG CCTTCTTCCT TCTCTTACTC CTGGTGTGGT TCCTCAACCG TGAGTTTGAA 
GTCAGCTACC GTCTTCATTA CCATGGCAAC GTAGAGGCTG ACAAACACCG CTTTAAGAAC 
CAGACGATGC GAGACCAGGC TGAGTGGTTA CTGGGCAACA TCATCCCCAT CCATGTGGCC 
GAACAGCTCA AGGTGACCCA GAGCTACTCA AAGAACCACG ACAGTGTGGG CGTGATCTTT 
GCCAGCATCG TAAACTTCAG CGAGTTTTAT GAGGAGACCT ACGAAGGTGG TAAGGAGTGC 
TACCGTGTCC TCAACGAGCT CATCGGAGAC TTTGACGAGC TGCTGCGCAA AGATGAATTC 
AAAAGCGTGG AAAAGATTAA AACGATAGGA GCAACCTACA TGGCTGCGTC TGGGCTGAAT 
GTCCAGCAGT TGGCTGAGAA TGACAATGAC TCTCCACACG CTCACCTCAG AGCCCTCTTT 
AACTTTGCCC TGGAGATGAT GGGAGTCCTG GAGGATTTTA ATAAGAACAT GCTGGGTTTC 
GGGTTCAAAC TCCGTATTGG ATTTAACCAC GGGCCACTGA CAGCAGGGGT GATCGGCACC 
ACTAAGTTGC TCTATGACAT CTGGGGAGAC ACTGTCAACA TTGCGAGCCG CATGGACTCT 
ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC GAGGAGAGCC ACGCTGTGCT CAGCGCCATG 
GGATTGGAGT TTGACTATAG AGGTACAGTG AATGTTAAAG GCAAAGGTCA AATGAGGACC 
TATCTCTACC CCAAAAGTGG GGAAAGCATA TTTCAAGATA AAATAGAACT GGGTCCCAGT 
GACGGACATT CACCCATGGC ATCAACAGCC ATTAAGACTC CAGGATCAGC TGCTCAAGCA 
GCAGGTCTTT CTCCTTATTC CTCCCACCTT TCCACAGACC CTCCCCAACC ACAAAGTGCT 
GTAGGGCCTT CAATTACAGG ACCTGAGCAT GACCATGCTC AGTTCACAGA GGTGAGGGAG 
GAAGGGTATA GGGACGCTCC ACCACCCCCG GGGCAGCCCA CGGCCCCAGA CCCTCACTCC 
CTGCCCCGCT CTGAACTCGT CCCAGAACCT AGTTGTGCAG AAGTGGAATC CCACGTGGAG 
CCTTCTCCTC TCACGCTCCA AGAACAGAAA GAAGAGTGGG AGGCTAACGA GCTAACCAAA 
CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610824.1
CDS	2067..6416
Translation	MASLQHHQPLMHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKVGSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRYRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_011612522.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612522.2

ORF Insert Sequence:

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGTAC AGGATGAACA CATTGTCGTC TGGTGGTTCA 
CCATCAGTCA CCCCTGGCCC TGACCCTCCT CCAGAACCTC TGGACATTCT AATCCCAGAA 
GCTGTTCTTG CCTTCTTCCT TCTCTTACTC CTGGTGTGGT TCCTCAACCG TGAGTTTGAA 
GTCAGCTACC GTCTTCATTA CCATGGCAAC GTAGAGGCTG ACAAACACCG CTTTAAGAAC 
CAGACGATGC GAGACCAGGC TGAGTGGTTA CTGGGCAACA TCATCCCCAT CCATGTGGCC 
GAACAGCTCA AGGTGACCCA GAGCTACTCA AAGAACCACG ACAGTGTGGG CGTGATCTTT 
GCCAGCATCG TAAACTTCAG CGAGTTTTAT GAGGAGACCT ACGAAGGTGG TAAGGAGTGC 
TACCGTGTCC TCAACGAGCT CATCGGAGAC TTTGACGAGC TGCTGCGCAA AGATGAATTC 
AAAAGCGTGG AAAAGATTAA AACGATAGGA GCAACCTACA TGGCTGCGTC TGGGCTGAAT 
GTCCAGCAGT TGGCTGAGAA TGACAATGAC TCTCCACACG CTCACCTCAG AGCCCTCTTT 
AACTTTGCCC TGGAGATGAT GGGAGTCCTG GAGGATTTTA ATAAGAACAT GCTGGGTTTC 
GGGTTCAAAC TCCGTATTGG ATTTAACCAC GGGCCACTGA CAGCAGGGGT GATCGGCACC 
ACTAAGTTGC TCTATGACAT CTGGGGAGAC ACTGTCAACA TTGCGAGCCG CATGGACTCT 
ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC GAGGAGAGCC ACGCTGTGCT CAGCGCCATG 
GGATTGGAGT TTGACTATAG AGGTACAGTG AATGTTAAAG GCAAAGGTCA AATGAGGACC 
TATCTCTACC CCAAAAGTGG GGAAAGCATA TTTCAAGATA AAATAGAACT GGGTCCCAGT 
GACGGACATT CACCCATGGC ATCAACAGCC ATTAAGACTC CAGGATCAGC TGCTCAAGCA 
GCAGGTCTTT CTCCTTATTC CTCCCACCTT TCCACAGACC CTCCCCAACC ACAAAGTGCT 
GTAGGGCCTT CAATTACAGG ACCTGAGCAT GACCATGCTC AGTTCACAGA GGTGAGGGAG 
GAAGGGTATA GGGACGCTCC ACCACCCCCG GGGCAGCCCA CGGCCCCAGA CCCTCACTCC 
CTGCCCCGCT CTGAACTCGT CCCAGAACCT AGTTGTGCAG AAGTGGAATC CCACGTGGAG 
CCTTCTCCTC TCACGCTCCA AGAACAGAAA GAAGAGTGGG AGGCTAACGA GCTAACCAAA 
CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074204 ( XM_029850688.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029706548.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo52876
Clone ID Related Accession (Same CDS sequence)	XM_011612522.2 , XM_029850688.1
Accession Version	XM_029850688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	adenylate cyclase type 9-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGTAC AGGATGAACA CATTGTCGTC TGGTGGTTCA 
CCATCAGTCA CCCCTGGCCC TGACCCTCCT CCAGAACCTC TGGACATTCT AATCCCAGAA 
GCTGTTCTTG CCTTCTTCCT TCTCTTACTC CTGGTGTGGT TCCTCAACCG TGAGTTTGAA 
GTCAGCTACC GTCTTCATTA CCATGGCAAC GTAGAGGCTG ACAAACACCG CTTTAAGAAC 
CAGACGATGC GAGACCAGGC TGAGTGGTTA CTGGGCAACA TCATCCCCAT CCATGTGGCC 
GAACAGCTCA AGGTGACCCA GAGCTACTCA AAGAACCACG ACAGTGTGGG CGTGATCTTT 
GCCAGCATCG TAAACTTCAG CGAGTTTTAT GAGGAGACCT ACGAAGGTGG TAAGGAGTGC 
TACCGTGTCC TCAACGAGCT CATCGGAGAC TTTGACGAGC TGCTGCGCAA AGATGAATTC 
AAAAGCGTGG AAAAGATTAA AACGATAGGA GCAACCTACA TGGCTGCGTC TGGGCTGAAT 
GTCCAGCAGT TGGCTGAGAA TGACAATGAC TCTCCACACG CTCACCTCAG AGCCCTCTTT 
AACTTTGCCC TGGAGATGAT GGGAGTCCTG GAGGATTTTA ATAAGAACAT GCTGGGTTTC 
GGGTTCAAAC TCCGTATTGG ATTTAACCAC GGGCCACTGA CAGCAGGGGT GATCGGCACC 
ACTAAGTTGC TCTATGACAT CTGGGGAGAC ACTGTCAACA TTGCGAGCCG CATGGACTCT 
ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC GAGGAGAGCC ACGCTGTGCT CAGCGCCATG 
GGATTGGAGT TTGACTATAG AGGTACAGTG AATGTTAAAG GCAAAGGTCA AATGAGGACC 
TATCTCTACC CCAAAAGTGG GGAAAGCATA TTTCAAGATA AAATAGAACT GGGTCCCAGT 
GACGGACATT CACCCATGGC ATCAACAGCC ATTAAGACTC CAGGATCAGC TGCTCAAGCA 
GCAGGTCTTT CTCCTTATTC CTCCCACCTT TCCACAGACC CTCCCCAACC ACAAAGTGCT 
GTAGGGCCTT CAATTACAGG ACCTGAGCAT GACCATGCTC AGTTCACAGA GGTGAGGGAG 
GAAGGGTATA GGGACGCTCC ACCACCCCCG GGGCAGCCCA CGGCCCCAGA CCCTCACTCC 
CTGCCCCGCT CTGAACTCGT CCCAGAACCT AGTTGTGCAG AAGTGGAATC CCACGTGGAG 
CCTTCTCCTC TCACGCTCCA AGAACAGAAA GAAGAGTGGG AGGCTAACGA GCTAACCAAA 
CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029706548.1
CDS	2070..6419
Translation	MASLQHHQPLMHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKVGSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRYRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_029850688.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029850688.1

ORF Insert Sequence:

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGTAC AGGATGAACA CATTGTCGTC TGGTGGTTCA 
CCATCAGTCA CCCCTGGCCC TGACCCTCCT CCAGAACCTC TGGACATTCT AATCCCAGAA 
GCTGTTCTTG CCTTCTTCCT TCTCTTACTC CTGGTGTGGT TCCTCAACCG TGAGTTTGAA 
GTCAGCTACC GTCTTCATTA CCATGGCAAC GTAGAGGCTG ACAAACACCG CTTTAAGAAC 
CAGACGATGC GAGACCAGGC TGAGTGGTTA CTGGGCAACA TCATCCCCAT CCATGTGGCC 
GAACAGCTCA AGGTGACCCA GAGCTACTCA AAGAACCACG ACAGTGTGGG CGTGATCTTT 
GCCAGCATCG TAAACTTCAG CGAGTTTTAT GAGGAGACCT ACGAAGGTGG TAAGGAGTGC 
TACCGTGTCC TCAACGAGCT CATCGGAGAC TTTGACGAGC TGCTGCGCAA AGATGAATTC 
AAAAGCGTGG AAAAGATTAA AACGATAGGA GCAACCTACA TGGCTGCGTC TGGGCTGAAT 
GTCCAGCAGT TGGCTGAGAA TGACAATGAC TCTCCACACG CTCACCTCAG AGCCCTCTTT 
AACTTTGCCC TGGAGATGAT GGGAGTCCTG GAGGATTTTA ATAAGAACAT GCTGGGTTTC 
GGGTTCAAAC TCCGTATTGG ATTTAACCAC GGGCCACTGA CAGCAGGGGT GATCGGCACC 
ACTAAGTTGC TCTATGACAT CTGGGGAGAC ACTGTCAACA TTGCGAGCCG CATGGACTCT 
ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC GAGGAGAGCC ACGCTGTGCT CAGCGCCATG 
GGATTGGAGT TTGACTATAG AGGTACAGTG AATGTTAAAG GCAAAGGTCA AATGAGGACC 
TATCTCTACC CCAAAAGTGG GGAAAGCATA TTTCAAGATA AAATAGAACT GGGTCCCAGT 
GACGGACATT CACCCATGGC ATCAACAGCC ATTAAGACTC CAGGATCAGC TGCTCAAGCA 
GCAGGTCTTT CTCCTTATTC CTCCCACCTT TCCACAGACC CTCCCCAACC ACAAAGTGCT 
GTAGGGCCTT CAATTACAGG ACCTGAGCAT GACCATGCTC AGTTCACAGA GGTGAGGGAG 
GAAGGGTATA GGGACGCTCC ACCACCCCCG GGGCAGCCCA CGGCCCCAGA CCCTCACTCC 
CTGCCCCGCT CTGAACTCGT CCCAGAACCT AGTTGTGCAG AAGTGGAATC CCACGTGGAG 
CCTTCTCCTC TCACGCTCCA AGAACAGAAA GAAGAGTGGG AGGCTAACGA GCTAACCAAA 
CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074204 ( XM_011612523.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610825.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X3, mRNA.

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CloneID	OTo52877
Clone ID Related Accession (Same CDS sequence)	XM_011612523.2
Accession Version	XM_011612523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	adenylate cyclase type 9-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGT CTCCCTGTGT TTCCCACTGT 
GTGAGCAAGT CAGCAGCAGA ACCGGGTGAT GTCGTAACTG TGCTCAACAG CTGTCAAGAA 
GAGAATAAGA CCAACACCTC CAGGCTCATC CCTGGTCGCA GATACAGAGC TGCCAACGGT 
CTCCTCAGCC CACCGGTAGA AGACCCCGTG AGAGCCAGCC AGAGCTCCCT GTGTGATATT 
CTCCAGGAGA AAGACAAAAA GACCAGTACA AGCACAGGGA CCAGCGCCAG CTTAGGCCTT 
GCTGGTGGAA CGGGGACAGC CGGTACTGGA ATGGACCACT CTGCTCTGAT CCACCTGCGA 
GCGAAAAACT TCAGAGAGAA GAGTGACGCT CACTTTGTGG ACGTTATCAG GGAGGATAGT 
CTGAAGGACT ACTTTTTCAA ACCGCCCATC AACAAGATCA GCCTTAATTT CCTAGAGAGA 
CCTCTGGAAA AGGCCTATCA CAGCAGCTAC AAAGAGGAGG TGAAGAACCA AGTCCAGGTA 
GAGACATTTG CAAGCTCTAC CTTCAGCTCT TTCCTGGACG TTCTCCTCAG CTGTTTCGTA 
TTCATGGCCC TGACACTGGC CTGTTTCCTT CCTCCCCTGG TGAGCCCGGC CACCGTAGGC 
CCACCAGCCA CCGCTGCCGT CACCCTGGCT GCCCTGGCCG GGCTGTTGGA AGTGGCATCT 
GTGGTGCTGT CTGTACGTAT GGCCTTCTGT CTGGAGGAGG TAATGAACTG TACCAACTCC 
CTTCTCCTGG TGGTCTCTGG ATGGATCCCC CGTCATGTGA TTGGAGCAGT GTTGATCTCT 
CTCCCTTCTA TCTCTGTCCT GTCCCACCTC TCCTGCAGTG TCCACTTCCC TCTACAGGTG 
ACCATGTTGC TCTGCTGTGC AACCATCCTC GCCATCATCC AGTATTGTAA CTTCTGTCAG 
CTCAGTTTTT GGATGCGTTC AGTCTTGGCC ACAGCCACAG GGGTCATCCT GCTGCTGGTT 
CTGTACAGCC CCTTGCACAG GTACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCACGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCACGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610825.1
CDS	2067..6410
Translation	MASLQHHQPLMHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRYRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_011612523.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612523.2

ORF Insert Sequence:

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGT CTCCCTGTGT TTCCCACTGT 
GTGAGCAAGT CAGCAGCAGA ACCGGGTGAT GTCGTAACTG TGCTCAACAG CTGTCAAGAA 
GAGAATAAGA CCAACACCTC CAGGCTCATC CCTGGTCGCA GATACAGAGC TGCCAACGGT 
CTCCTCAGCC CACCGGTAGA AGACCCCGTG AGAGCCAGCC AGAGCTCCCT GTGTGATATT 
CTCCAGGAGA AAGACAAAAA GACCAGTACA AGCACAGGGA CCAGCGCCAG CTTAGGCCTT 
GCTGGTGGAA CGGGGACAGC CGGTACTGGA ATGGACCACT CTGCTCTGAT CCACCTGCGA 
GCGAAAAACT TCAGAGAGAA GAGTGACGCT CACTTTGTGG ACGTTATCAG GGAGGATAGT 
CTGAAGGACT ACTTTTTCAA ACCGCCCATC AACAAGATCA GCCTTAATTT CCTAGAGAGA 
CCTCTGGAAA AGGCCTATCA CAGCAGCTAC AAAGAGGAGG TGAAGAACCA AGTCCAGGTA 
GAGACATTTG CAAGCTCTAC CTTCAGCTCT TTCCTGGACG TTCTCCTCAG CTGTTTCGTA 
TTCATGGCCC TGACACTGGC CTGTTTCCTT CCTCCCCTGG TGAGCCCGGC CACCGTAGGC 
CCACCAGCCA CCGCTGCCGT CACCCTGGCT GCCCTGGCCG GGCTGTTGGA AGTGGCATCT 
GTGGTGCTGT CTGTACGTAT GGCCTTCTGT CTGGAGGAGG TAATGAACTG TACCAACTCC 
CTTCTCCTGG TGGTCTCTGG ATGGATCCCC CGTCATGTGA TTGGAGCAGT GTTGATCTCT 
CTCCCTTCTA TCTCTGTCCT GTCCCACCTC TCCTGCAGTG TCCACTTCCC TCTACAGGTG 
ACCATGTTGC TCTGCTGTGC AACCATCCTC GCCATCATCC AGTATTGTAA CTTCTGTCAG 
CTCAGTTTTT GGATGCGTTC AGTCTTGGCC ACAGCCACAG GGGTCATCCT GCTGCTGGTT 
CTGTACAGCC CCTTGCACAG GTACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCACGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCACGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074204 ( XM_011612524.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610826.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X4, mRNA.

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CloneID	OTo52878
Clone ID Related Accession (Same CDS sequence)	XM_011612524.2
Accession Version	XM_011612524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	adenylate cyclase type 9-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCACGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCACGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610826.1
CDS	2067..6410
Translation	MASLQHHQPLMHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKVGSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_011612524.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612524.2

ORF Insert Sequence:

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCCTTTTT AGTGCTCCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA CGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCACGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCACGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074204 ( XM_029850689.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029706549.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X5, mRNA.

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CloneID	OTo52879
Clone ID Related Accession (Same CDS sequence)	XM_029850689.1
Accession Version	XM_029850689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	adenylate cyclase type 9-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGCA CAGAAGTGAG CTGTGACTCC AGCGGGGATG GCGCCGTCAC CGTGCAGATC 
ACCAACAGCG TGCCGCACAT GCATCGCGAG CCCCAGGCTG GAGCGGGAGG AGCGAAAACG 
AACCCCAACC TGCACAAATA CAGCATCTCT TCCAGCTGCA GCTCGGCGGA CTCCGGAACG 
GCCGCGGCGA CCACGTCCAG GGTGCGTCGG AGAGTGCCGC CATTGTTTGA GCGCTCCGCC 
GCTCAGTGCT GGGACCCCAA ATTTGATTCG CCCATTCTGG AAGAGGCTTG CCAGGAGAGG 
TGCTTTCCTC AGACGCAGCG GCGCTTTCGT TACGTTCTGT CGTACCTGGC AGTGGCAGCT 
GTTCTTTGGG GGGTTTACTT TGGAGTCAAT AGTGACCGCT GCAGCCCTGT GGCCTTTTTA 
GCCCCAACAG CATCCTTTTT AGTGCTCCTC ATGCTCCTCC TGTTGTTCAC CTTTACCCAA 
CCCTACACCT GGCTGTACAA CCAAACGTCC TTGTTTCTAA TCGCCGTTAC CTTCGCCATC 
ACTCTGGCGC CACAGATACA GACTGCTGGC TACATTCAGC TGGGGTGGGC TGCTGGACTG 
AACGCCTCGT TCCTCTCACC CGACAGAGTT GCCCCGCTCC CAACCCCTTG CATCTCCCCT 
GTTGGCACCT TTTCGTTGTG CATGGAGGTG CTGTTGTTGC TGTACAGCGT CCTCCACGTC 
CGCCTGTATG CGTCAGTGAT GCTGGGGTTC TTATATTCTG TTTTGTTTGA GGCGTTAGAA 
TGGCTGCCAT TGCTTCAGAA GAGCTATGAC ACTACGGGGT GGCAGACAGA ATCGGTCGGC 
TCGGGTTCTG ATTGGGAAGG ACACACCCTC CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC 
CACTTGTGCG CCCATGTGAT TGGCATCCAC CTGTTCATCA TGTCAGAGGT ACGCTCCCGC 
AGCACCTTCC TCAAAGTGGG CCAGGCCATC ATGCACGGCA AAGACCTGGA GGTCGAAAAG 
GCCTTGAAGG AACGTATGAT TCACTCCGTA ATGCCACGGC GGGTTGCGGA CGATCTCCTG 
AAGCAGGGGG ACGACGAGGG CTGCGGCGAG AGTTCCGGCA AGCGCTACTC TTCCGGCGCC 
TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC GGGGGAGGCG GCGGATTGTC GCAAGCCCAG 
AGCGTCACAA ACGCTAAGCA CGGCCACAAC AATCACAAAA GCAAAAAAAC CTCCATTCCC 
CGCGGACAGA TCATCTTTCG GCCCTTCAAC ATGAAGCGCA TGGAGCCTGT CAGCATCCTC 
TTCGCCGATA TCGTGGGTTT CACCAAAATG TCGGCCAATA AGTCGGCGCC GGCCCTGGTA 
GGCCTTCTCA ATGATCTCTT CGGACGTTTT GACCGGCTGT GCGAGCTTAC TGGTTGCGAG 
AAGATCAGCA CTCTGGGAGA CTGCTATTAT TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG 
GACCACGCCT ACTGCTGTGT GGAAATGGGC TTGGGGATGA TCCAGGCCAT CGAACAGTTC 
TGCCAGGAGA CGCGCGAGAC CGTCAGCATG CGCGTTGGGG TCCATACCGG CACCGTCCTG 
TGCGGGATCC TGGGAATGAA GCGTTTTAAG TTTGATGTCT GGTCAAATGA CGTCAATCTG 
GCCAATCTGA TGGAGCAGCT GGGTGTGGCG GGCAAGATCC ACCTGTCAGA AGCAACTGCC 
CAGTTCCTGG GTGACCGTTA CCAGAGGGAA GACGGGCGAG TGTTGGAGAG AGCGGGGCAG 
AGCGCGGTGG AGAAACTCAA GGGGATGAAG ACCTACCTGA TCTCAGGAAA GAAGCTGGAG 
CGCGACGTCC CGTGTGGCTG TTCTGACTTT GTCAGCCGGT CGCCACACCC ACAGCCCCGC 
ACGCCAGACC TGATTTCTAG AGGAGTGCAG GCAGCTCAGG TGCTACTGAC CCACACAGGC 
CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC CACTGTGTGA GCAAGTCAGC AGCAGAACCG 
GGTGATGTCG TAACTGTGCT CAACAGCTGT CAAGAAGAGA ATAAGACCAA CACCTCCAGG 
CTCATCCCTG GTCGCAGATA CAGAGCTGCC AACGGTCTCC TCAGCCCACC GGTAGAAGAC 
CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT GATATTCTCC AGGAGAAAGA CAAAAAGACC 
AGTACAAGCA CAGGGACCAG CGCCAGCTTA GGCCTTGCTG GTGGAACGGG GACAGCCGGT 
ACTGGAATGG ACCACTCTGC TCTGATCCAC CTGCGAGCGA AAAACTTCAG AGAGAAGAGT 
GACGCTCACT TTGTGGACGT TATCAGGGAG GATAGTCTGA AGGACTACTT TTTCAAACCG 
CCCATCAACA AGATCAGCCT TAATTTCCTA GAGAGACCTC TGGAAAAGGC CTATCACAGC 
AGCTACAAAG AGGAGGTGAA GAACCAAGTC CAGGTAGAGA CATTTGCAAG CTCTACCTTC 
AGCTCTTTCC TGGACGTTCT CCTCAGCTGT TTCGTATTCA TGGCCCTGAC ACTGGCCTGT 
TTCCTTCCTC CCCTGGTGAG CCCGGCCACC GTAGGCCCAC CAGCCACCGC TGCCGTCACC 
CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG GCATCTGTGG TGCTGTCTGT ACGTATGGCC 
TTCTGTCTGG AGGAGGTAAT GAACTGTACC AACTCCCTTC TCCTGGTGGT CTCTGGATGG 
ATCCCCCGTC ATGTGATTGG AGCAGTGTTG ATCTCTCTCC CTTCTATCTC TGTCCTGTCC 
CACCTCTCCT GCAGTGTCCA CTTCCCTCTA CAGGTGACCA TGTTGCTCTG CTGTGCAACC 
ATCCTCGCCA TCATCCAGTA TTGTAACTTC TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC 
TTGGCCACAG CCACAGGGGT CATCCTGCTG CTGGTTCTGT ACAGCCCCTT GCACAGGTAC 
AGGATGAACA CATTGTCGTC TGGTGGTTCA CCATCAGTCA CCCCTGGCCC TGACCCTCCT 
CCAGAACCTC TGGACATTCT AATCCCAGAA GCTGTTCTTG CCTTCTTCCT TCTCTTACTC 
CTGGTGTGGT TCCTCAACCG TGAGTTTGAA GTCAGCTACC GTCTTCATTA CCATGGCAAC 
GTAGAGGCTG ACAAACACCG CTTTAAGAAC CAGACGATGC GAGACCAGGC TGAGTGGTTA 
CTGGGCAACA TCATCCCCAT CCATGTGGCC GAACAGCTCA AGGTGACCCA GAGCTACTCA 
AAGAACCACG ACAGTGTGGG CGTGATCTTT GCCAGCATCG TAAACTTCAG CGAGTTTTAT 
GAGGAGACCT ACGAAGGTGG TAAGGAGTGC TACCGTGTCC TCAACGAGCT CATCGGAGAC 
TTTGACGAGC TGCTGCGCAA AGATGAATTC AAAAGCGTGG AAAAGATTAA AACGATAGGA 
GCAACCTACA TGGCTGCGTC TGGGCTGAAT GTCCAGCAGT TGGCTGAGAA TGACAATGAC 
TCTCCACACG CTCACCTCAG AGCCCTCTTT AACTTTGCCC TGGAGATGAT GGGAGTCCTG 
GAGGATTTTA ATAAGAACAT GCTGGGTTTC GGGTTCAAAC TCCGTATTGG ATTTAACCAC 
GGGCCACTGA CAGCAGGGGT GATCGGCACC ACTAAGTTGC TCTATGACAT CTGGGGAGAC 
ACTGTCAACA TTGCGAGCCG CATGGACTCT ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC 
GAGGAGAGCC ACGCTGTGCT CAGCGCCATG GGATTGGAGT TTGACTATAG AGGTACAGTG 
AATGTTAAAG GCAAAGGTCA AATGAGGACC TATCTCTACC CCAAAAGTGG GGAAAGCATA 
TTTCAAGATA AAATAGAACT GGGTCCCAGT GACGGACATT CACCCATGGC ATCAACAGCC 
ATTAAGACTC CAGGATCAGC TGCTCAAGCA GCAGGTCTTT CTCCTTATTC CTCCCACCTT 
TCCACAGACC CTCCCCAACC ACAAAGTGCT GTAGGGCCTT CAATTACAGG ACCTGAGCAT 
GACCATGCTC AGTTCACAGA GGTGAGGGAG GAAGGGTATA GGGACGCTCC ACCACCCCCG 
GGGCAGCCCA CGGCCCCAGA CCCTCACTCC CTGCCCCGCT CTGAACTCGT CCCAGAACCT 
AGTTGTGCAG AAGTGGAATC CCACGTGGAG CCTTCTCCTC TCACGCTCCA AGAACAGAAA 
GAAGAGTGGG AGGCTAACGA GCTAACCAAA CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029706549.1
CDS	1176..5495
Translation	MHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKVGSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRYRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_029850689.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029850689.1

ORF Insert Sequence:

ATGCATGGCA CAGAAGTGAG CTGTGACTCC AGCGGGGATG GCGCCGTCAC CGTGCAGATC 
ACCAACAGCG TGCCGCACAT GCATCGCGAG CCCCAGGCTG GAGCGGGAGG AGCGAAAACG 
AACCCCAACC TGCACAAATA CAGCATCTCT TCCAGCTGCA GCTCGGCGGA CTCCGGAACG 
GCCGCGGCGA CCACGTCCAG GGTGCGTCGG AGAGTGCCGC CATTGTTTGA GCGCTCCGCC 
GCTCAGTGCT GGGACCCCAA ATTTGATTCG CCCATTCTGG AAGAGGCTTG CCAGGAGAGG 
TGCTTTCCTC AGACGCAGCG GCGCTTTCGT TACGTTCTGT CGTACCTGGC AGTGGCAGCT 
GTTCTTTGGG GGGTTTACTT TGGAGTCAAT AGTGACCGCT GCAGCCCTGT GGCCTTTTTA 
GCCCCAACAG CATCCTTTTT AGTGCTCCTC ATGCTCCTCC TGTTGTTCAC CTTTACCCAA 
CCCTACACCT GGCTGTACAA CCAAACGTCC TTGTTTCTAA TCGCCGTTAC CTTCGCCATC 
ACTCTGGCGC CACAGATACA GACTGCTGGC TACATTCAGC TGGGGTGGGC TGCTGGACTG 
AACGCCTCGT TCCTCTCACC CGACAGAGTT GCCCCGCTCC CAACCCCTTG CATCTCCCCT 
GTTGGCACCT TTTCGTTGTG CATGGAGGTG CTGTTGTTGC TGTACAGCGT CCTCCACGTC 
CGCCTGTATG CGTCAGTGAT GCTGGGGTTC TTATATTCTG TTTTGTTTGA GGCGTTAGAA 
TGGCTGCCAT TGCTTCAGAA GAGCTATGAC ACTACGGGGT GGCAGACAGA ATCGGTCGGC 
TCGGGTTCTG ATTGGGAAGG ACACACCCTC CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC 
CACTTGTGCG CCCATGTGAT TGGCATCCAC CTGTTCATCA TGTCAGAGGT ACGCTCCCGC 
AGCACCTTCC TCAAAGTGGG CCAGGCCATC ATGCACGGCA AAGACCTGGA GGTCGAAAAG 
GCCTTGAAGG AACGTATGAT TCACTCCGTA ATGCCACGGC GGGTTGCGGA CGATCTCCTG 
AAGCAGGGGG ACGACGAGGG CTGCGGCGAG AGTTCCGGCA AGCGCTACTC TTCCGGCGCC 
TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC GGGGGAGGCG GCGGATTGTC GCAAGCCCAG 
AGCGTCACAA ACGCTAAGCA CGGCCACAAC AATCACAAAA GCAAAAAAAC CTCCATTCCC 
CGCGGACAGA TCATCTTTCG GCCCTTCAAC ATGAAGCGCA TGGAGCCTGT CAGCATCCTC 
TTCGCCGATA TCGTGGGTTT CACCAAAATG TCGGCCAATA AGTCGGCGCC GGCCCTGGTA 
GGCCTTCTCA ATGATCTCTT CGGACGTTTT GACCGGCTGT GCGAGCTTAC TGGTTGCGAG 
AAGATCAGCA CTCTGGGAGA CTGCTATTAT TGTGTGGCCG GCTGTCCCGA GCCGAGCCCG 
GACCACGCCT ACTGCTGTGT GGAAATGGGC TTGGGGATGA TCCAGGCCAT CGAACAGTTC 
TGCCAGGAGA CGCGCGAGAC CGTCAGCATG CGCGTTGGGG TCCATACCGG CACCGTCCTG 
TGCGGGATCC TGGGAATGAA GCGTTTTAAG TTTGATGTCT GGTCAAATGA CGTCAATCTG 
GCCAATCTGA TGGAGCAGCT GGGTGTGGCG GGCAAGATCC ACCTGTCAGA AGCAACTGCC 
CAGTTCCTGG GTGACCGTTA CCAGAGGGAA GACGGGCGAG TGTTGGAGAG AGCGGGGCAG 
AGCGCGGTGG AGAAACTCAA GGGGATGAAG ACCTACCTGA TCTCAGGAAA GAAGCTGGAG 
CGCGACGTCC CGTGTGGCTG TTCTGACTTT GTCAGCCGGT CGCCACACCC ACAGCCCCGC 
ACGCCAGACC TGATTTCTAG AGGAGTGCAG GCAGCTCAGG TGCTACTGAC CCACACAGGC 
CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC CACTGTGTGA GCAAGTCAGC AGCAGAACCG 
GGTGATGTCG TAACTGTGCT CAACAGCTGT CAAGAAGAGA ATAAGACCAA CACCTCCAGG 
CTCATCCCTG GTCGCAGATA CAGAGCTGCC AACGGTCTCC TCAGCCCACC GGTAGAAGAC 
CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT GATATTCTCC AGGAGAAAGA CAAAAAGACC 
AGTACAAGCA CAGGGACCAG CGCCAGCTTA GGCCTTGCTG GTGGAACGGG GACAGCCGGT 
ACTGGAATGG ACCACTCTGC TCTGATCCAC CTGCGAGCGA AAAACTTCAG AGAGAAGAGT 
GACGCTCACT TTGTGGACGT TATCAGGGAG GATAGTCTGA AGGACTACTT TTTCAAACCG 
CCCATCAACA AGATCAGCCT TAATTTCCTA GAGAGACCTC TGGAAAAGGC CTATCACAGC 
AGCTACAAAG AGGAGGTGAA GAACCAAGTC CAGGTAGAGA CATTTGCAAG CTCTACCTTC 
AGCTCTTTCC TGGACGTTCT CCTCAGCTGT TTCGTATTCA TGGCCCTGAC ACTGGCCTGT 
TTCCTTCCTC CCCTGGTGAG CCCGGCCACC GTAGGCCCAC CAGCCACCGC TGCCGTCACC 
CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG GCATCTGTGG TGCTGTCTGT ACGTATGGCC 
TTCTGTCTGG AGGAGGTAAT GAACTGTACC AACTCCCTTC TCCTGGTGGT CTCTGGATGG 
ATCCCCCGTC ATGTGATTGG AGCAGTGTTG ATCTCTCTCC CTTCTATCTC TGTCCTGTCC 
CACCTCTCCT GCAGTGTCCA CTTCCCTCTA CAGGTGACCA TGTTGCTCTG CTGTGCAACC 
ATCCTCGCCA TCATCCAGTA TTGTAACTTC TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC 
TTGGCCACAG CCACAGGGGT CATCCTGCTG CTGGTTCTGT ACAGCCCCTT GCACAGGTAC 
AGGATGAACA CATTGTCGTC TGGTGGTTCA CCATCAGTCA CCCCTGGCCC TGACCCTCCT 
CCAGAACCTC TGGACATTCT AATCCCAGAA GCTGTTCTTG CCTTCTTCCT TCTCTTACTC 
CTGGTGTGGT TCCTCAACCG TGAGTTTGAA GTCAGCTACC GTCTTCATTA CCATGGCAAC 
GTAGAGGCTG ACAAACACCG CTTTAAGAAC CAGACGATGC GAGACCAGGC TGAGTGGTTA 
CTGGGCAACA TCATCCCCAT CCATGTGGCC GAACAGCTCA AGGTGACCCA GAGCTACTCA 
AAGAACCACG ACAGTGTGGG CGTGATCTTT GCCAGCATCG TAAACTTCAG CGAGTTTTAT 
GAGGAGACCT ACGAAGGTGG TAAGGAGTGC TACCGTGTCC TCAACGAGCT CATCGGAGAC 
TTTGACGAGC TGCTGCGCAA AGATGAATTC AAAAGCGTGG AAAAGATTAA AACGATAGGA 
GCAACCTACA TGGCTGCGTC TGGGCTGAAT GTCCAGCAGT TGGCTGAGAA TGACAATGAC 
TCTCCACACG CTCACCTCAG AGCCCTCTTT AACTTTGCCC TGGAGATGAT GGGAGTCCTG 
GAGGATTTTA ATAAGAACAT GCTGGGTTTC GGGTTCAAAC TCCGTATTGG ATTTAACCAC 
GGGCCACTGA CAGCAGGGGT GATCGGCACC ACTAAGTTGC TCTATGACAT CTGGGGAGAC 
ACTGTCAACA TTGCGAGCCG CATGGACTCT ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC 
GAGGAGAGCC ACGCTGTGCT CAGCGCCATG GGATTGGAGT TTGACTATAG AGGTACAGTG 
AATGTTAAAG GCAAAGGTCA AATGAGGACC TATCTCTACC CCAAAAGTGG GGAAAGCATA 
TTTCAAGATA AAATAGAACT GGGTCCCAGT GACGGACATT CACCCATGGC ATCAACAGCC 
ATTAAGACTC CAGGATCAGC TGCTCAAGCA GCAGGTCTTT CTCCTTATTC CTCCCACCTT 
TCCACAGACC CTCCCCAACC ACAAAGTGCT GTAGGGCCTT CAATTACAGG ACCTGAGCAT 
GACCATGCTC AGTTCACAGA GGTGAGGGAG GAAGGGTATA GGGACGCTCC ACCACCCCCG 
GGGCAGCCCA CGGCCCCAGA CCCTCACTCC CTGCCCCGCT CTGAACTCGT CCCAGAACCT 
AGTTGTGCAG AAGTGGAATC CCACGTGGAG CCTTCTCCTC TCACGCTCCA AGAACAGAAA 
GAAGAGTGGG AGGCTAACGA GCTAACCAAA CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074204 ( XM_011612522.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610824.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo28129
Clone ID Related Accession (Same CDS sequence)	XM_011612522.1
Accession Version	XM_011612522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	adenylate cyclase type 9-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071110.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCGTTTTT AGTGCTGCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCCAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA TGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGTAC AGGATGAACA CATTGTCGTC TGGTGGTTCA 
CCATCAGTCA CCCCTGGCCC TGACCCTCCT CCAGAACCTC TGGACATTCT AATCCCAGAA 
GCTGTTCTTG CCTTCTTCCT TCTCTTACTC CTGGTGTGGT TCCTCAACCG TGAGTTTGAA 
GTCAGCTACC GTCTTCATTA CCATGGCAAC GTAGAGGCTG ACAAACACCG CTTTAAGAAC 
CAGACGATGC GAGACCAGGC TGAGTGGTTA CTGGGCAACA TCATCCCCAT CCATGTGGCC 
GAACAGCTCA AGGTGACCCA GAGCTACTCA AAGAACCACG ACAGTGTGGG CGTGATCTTT 
GCCAGCATCG TAAACTTCAG CGAGTTTTAT GAGGAGACCT ACGAAGGTGG TAAGGAGTGC 
TACCGTGTCC TCAACGAGCT CATCGGAGAC TTTGACGAGC TGCTGCGCAA AGATGAATTC 
AAAAGCGTGG AAAAGATTAA AACGATAGGA GCAACCTACA TGGCTGCGTC TGGGCTGAAT 
GTCCAGCAGT TGGCTGAGAA TGACAATGAC TCTCCACACG CTCACCTCAG AGCCCTCTTT 
AACTTTGCCC TGGAGATGAT GGGAGTCCTG GAGGATTTTA ATAAGAACAT GCTGGGTTTC 
GGGTTCAAAC TCCGTATTGG ATTTAACCAC GGGCCACTGA CAGCAGGGGT GATCGGCACC 
ACTAAGTTGC TCTATGACAT CTGGGGAGAC ACTGTCAACA TTGCGAGCCG CATGGACTCT 
ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC GAGGAGAGCC ATGCTGTGCT CAGCGCCATG 
GGATTGGAGT TTGACTATAG AGGTACAGTG AATGTTAAAG GCAAAGGTCA AATGAGGACC 
TATCTCTACC CCAAAAGTGG GGAAAGCATA TTTCAAGATA AAATAGAACT GGGTCCCAGT 
GACGGACATT CACCCATGGC ATCAACAGCC ATTAAGACTC CAGGATCAGC TGCTCAAGCA 
GCAGGTCTTT CTCCTTATTC CTCCCACCTT TCCACAGACC CTCCCCAACC ACAAAGTGCT 
GTAGGGCCTT CAATTACAGG ACCTGAGCAT GACCATGCTC AGTTCACAGA GGTGAGGGAG 
GAAGGGTATA GGGACGCTCC ACCACCCCCG GGGCAGCCCA CGGCCCCAGA CCCTCACTCC 
CTGCCCCGCT CTGAACTCGT CCCAGAACCT AGTTGTGCAG AAGTGGAATC CCATGTGGAG 
CCTTCTCCTC TCACGCTCCA AGAACAGAAA GAAGAGTGGG AGGCTAACGA GCTAACCAAA 
CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610824.1
CDS	2056..6405
Translation	MASLQHHQPLMHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKVGSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRYRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_011612522.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612522.1

ORF Insert Sequence:

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCGTTTTT AGTGCTGCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCCAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA TGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGTAC AGGATGAACA CATTGTCGTC TGGTGGTTCA 
CCATCAGTCA CCCCTGGCCC TGACCCTCCT CCAGAACCTC TGGACATTCT AATCCCAGAA 
GCTGTTCTTG CCTTCTTCCT TCTCTTACTC CTGGTGTGGT TCCTCAACCG TGAGTTTGAA 
GTCAGCTACC GTCTTCATTA CCATGGCAAC GTAGAGGCTG ACAAACACCG CTTTAAGAAC 
CAGACGATGC GAGACCAGGC TGAGTGGTTA CTGGGCAACA TCATCCCCAT CCATGTGGCC 
GAACAGCTCA AGGTGACCCA GAGCTACTCA AAGAACCACG ACAGTGTGGG CGTGATCTTT 
GCCAGCATCG TAAACTTCAG CGAGTTTTAT GAGGAGACCT ACGAAGGTGG TAAGGAGTGC 
TACCGTGTCC TCAACGAGCT CATCGGAGAC TTTGACGAGC TGCTGCGCAA AGATGAATTC 
AAAAGCGTGG AAAAGATTAA AACGATAGGA GCAACCTACA TGGCTGCGTC TGGGCTGAAT 
GTCCAGCAGT TGGCTGAGAA TGACAATGAC TCTCCACACG CTCACCTCAG AGCCCTCTTT 
AACTTTGCCC TGGAGATGAT GGGAGTCCTG GAGGATTTTA ATAAGAACAT GCTGGGTTTC 
GGGTTCAAAC TCCGTATTGG ATTTAACCAC GGGCCACTGA CAGCAGGGGT GATCGGCACC 
ACTAAGTTGC TCTATGACAT CTGGGGAGAC ACTGTCAACA TTGCGAGCCG CATGGACTCT 
ACAGGTGTGG AGTGTCGGGT GCAGGTGAGC GAGGAGAGCC ATGCTGTGCT CAGCGCCATG 
GGATTGGAGT TTGACTATAG AGGTACAGTG AATGTTAAAG GCAAAGGTCA AATGAGGACC 
TATCTCTACC CCAAAAGTGG GGAAAGCATA TTTCAAGATA AAATAGAACT GGGTCCCAGT 
GACGGACATT CACCCATGGC ATCAACAGCC ATTAAGACTC CAGGATCAGC TGCTCAAGCA 
GCAGGTCTTT CTCCTTATTC CTCCCACCTT TCCACAGACC CTCCCCAACC ACAAAGTGCT 
GTAGGGCCTT CAATTACAGG ACCTGAGCAT GACCATGCTC AGTTCACAGA GGTGAGGGAG 
GAAGGGTATA GGGACGCTCC ACCACCCCCG GGGCAGCCCA CGGCCCCAGA CCCTCACTCC 
CTGCCCCGCT CTGAACTCGT CCCAGAACCT AGTTGTGCAG AAGTGGAATC CCATGTGGAG 
CCTTCTCCTC TCACGCTCCA AGAACAGAAA GAAGAGTGGG AGGCTAACGA GCTAACCAAA 
CTCAACGAGG AGTTTTACGC TCGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074204 ( XM_011612523.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610825.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X2, mRNA.

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CloneID	OTo28130
Clone ID Related Accession (Same CDS sequence)	XM_011612523.1
Accession Version	XM_011612523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	adenylate cyclase type 9-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071110.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCGTTTTT AGTGCTGCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCCAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA TGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGT CTCCCTGTGT TTCCCACTGT 
GTGAGCAAGT CAGCAGCAGA ACCGGGTGAT GTCGTAACTG TGCTCAACAG CTGTCAAGAA 
GAGAATAAGA CCAACACCTC CAGGCTCATC CCTGGTCGCA GATACAGAGC TGCCAACGGT 
CTCCTCAGCC CACCGGTAGA AGACCCCGTG AGAGCCAGCC AGAGCTCCCT GTGTGATATT 
CTCCAGGAGA AAGACAAAAA GACCAGTACA AGCACAGGGA CCAGCGCCAG CTTAGGCCTT 
GCTGGTGGAA CGGGGACAGC CGGTACTGGA ATGGACCACT CTGCTCTGAT CCACCTGCGA 
GCGAAAAACT TCAGAGAGAA GAGTGACGCT CACTTTGTGG ACGTTATCAG GGAGGATAGT 
CTGAAGGACT ACTTTTTCAA ACCGCCCATC AACAAGATCA GCCTTAATTT CCTAGAGAGA 
CCTCTGGAAA AGGCCTATCA CAGCAGCTAC AAAGAGGAGG TGAAGAACCA AGTCCAGGTA 
GAGACATTTG CAAGCTCTAC CTTCAGCTCT TTCCTGGACG TTCTCCTCAG CTGTTTCGTA 
TTCATGGCCC TGACACTGGC CTGTTTCCTT CCTCCCCTGG TGAGCCCGGC CACCGTAGGC 
CCACCAGCCA CCGCTGCCGT CACCCTGGCT GCCCTGGCCG GGCTGTTGGA AGTGGCATCT 
GTGGTGCTGT CTGTACGTAT GGCCTTCTGT CTGGAGGAGG TAATGAACTG TACCAACTCC 
CTTCTCCTGG TGGTCTCTGG ATGGATCCCC CGTCATGTGA TTGGAGCAGT GTTGATCTCT 
CTCCCTTCTA TCTCTGTCCT GTCCCACCTC TCCTGCAGTG TCCACTTCCC TCTACAGGTG 
ACCATGTTGC TCTGCTGTGC AACCATCCTC GCCATCATCC AGTATTGTAA CTTCTGTCAG 
CTCAGTTTTT GGATGCGTTC AGTCTTGGCC ACAGCCACAG GGGTCATCCT GCTGCTGGTT 
CTGTACAGCC CCTTGCACAG GTACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCATGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCATGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610825.1
CDS	2056..6399
Translation	MASLQHHQPLMHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRYRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_011612523.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612523.1

ORF Insert Sequence:

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCGTTTTT AGTGCTGCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCCAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA TGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGT CTCCCTGTGT TTCCCACTGT 
GTGAGCAAGT CAGCAGCAGA ACCGGGTGAT GTCGTAACTG TGCTCAACAG CTGTCAAGAA 
GAGAATAAGA CCAACACCTC CAGGCTCATC CCTGGTCGCA GATACAGAGC TGCCAACGGT 
CTCCTCAGCC CACCGGTAGA AGACCCCGTG AGAGCCAGCC AGAGCTCCCT GTGTGATATT 
CTCCAGGAGA AAGACAAAAA GACCAGTACA AGCACAGGGA CCAGCGCCAG CTTAGGCCTT 
GCTGGTGGAA CGGGGACAGC CGGTACTGGA ATGGACCACT CTGCTCTGAT CCACCTGCGA 
GCGAAAAACT TCAGAGAGAA GAGTGACGCT CACTTTGTGG ACGTTATCAG GGAGGATAGT 
CTGAAGGACT ACTTTTTCAA ACCGCCCATC AACAAGATCA GCCTTAATTT CCTAGAGAGA 
CCTCTGGAAA AGGCCTATCA CAGCAGCTAC AAAGAGGAGG TGAAGAACCA AGTCCAGGTA 
GAGACATTTG CAAGCTCTAC CTTCAGCTCT TTCCTGGACG TTCTCCTCAG CTGTTTCGTA 
TTCATGGCCC TGACACTGGC CTGTTTCCTT CCTCCCCTGG TGAGCCCGGC CACCGTAGGC 
CCACCAGCCA CCGCTGCCGT CACCCTGGCT GCCCTGGCCG GGCTGTTGGA AGTGGCATCT 
GTGGTGCTGT CTGTACGTAT GGCCTTCTGT CTGGAGGAGG TAATGAACTG TACCAACTCC 
CTTCTCCTGG TGGTCTCTGG ATGGATCCCC CGTCATGTGA TTGGAGCAGT GTTGATCTCT 
CTCCCTTCTA TCTCTGTCCT GTCCCACCTC TCCTGCAGTG TCCACTTCCC TCTACAGGTG 
ACCATGTTGC TCTGCTGTGC AACCATCCTC GCCATCATCC AGTATTGTAA CTTCTGTCAG 
CTCAGTTTTT GGATGCGTTC AGTCTTGGCC ACAGCCACAG GGGTCATCCT GCTGCTGGTT 
CTGTACAGCC CCTTGCACAG GTACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCATGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCATGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074204 ( XM_011612524.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610826.1 Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X3, mRNA.

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CloneID	OTo28131
Clone ID Related Accession (Same CDS sequence)	XM_011612524.1
Accession Version	XM_011612524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	adenylate cyclase type 9-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071110.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCGTTTTT AGTGCTGCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCCAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA TGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCATGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCATGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610826.1
CDS	2056..6399
Translation	MASLQHHQPLMHGTEVSCDSSGDGAVTVQITNSVPHMHREPQAGAGGAKTNPNLHKYSISSSCSSADSGTAAATTSRVRRRVPPLFERSAAQCWDPKFDSPILEEACQERCFPQTQRRFRYVLSYLAVAAVLWGVYFGVNSDRCSPVAFLAPTASFLVLLMLLLLFTFTQPYTWLYNQTSLFLIAVTFAITLAPQIQTAGYIQLGWAAGLNASFLSPDRVAPLPTPCISPVGTFSLCMEVLLLLYSVLHVRLYASVMLGFLYSVLFEALEWLPLLQKSYDTTGWQTESVGSGSDWEGHTLRWLGPAKALLHLCAHVIGIHLFIMSEVRSRSTFLKVGQAIMHGKDLEVEKALKERMIHSVMPRRVADDLLKQGDDEGCGESSGKRYSSGACSASAVSVGGGGGGGLSQAQSVTNAKHGHNNHKSKKTSIPRGQIIFRPFNMKRMEPVSILFADIVGFTKMSANKSAPALVGLLNDLFGRFDRLCELTGCEKISTLGDCYYCVAGCPEPSPDHAYCCVEMGLGMIQAIEQFCQETRETVSMRVGVHTGTVLCGILGMKRFKFDVWSNDVNLANLMEQLGVAGKIHLSEATAQFLGDRYQREDGRVLERAGQSAVEKLKGMKTYLISGKKLERDVPCGCSDFVSRSPHPQPRTPDLISRGVQAAQVLLTHTGPEKVGSPCVSHCVSKSAAEPGDVVTVLNSCQEENKTNTSRLIPGRRYRAANGLLSPPVEDPVRASQSSLCDILQEKDKKTSTSTGTSASLGLAGGTGTAGTGMDHSALIHLRAKNFREKSDAHFVDVIREDSLKDYFFKPPINKISLNFLERPLEKAYHSSYKEEVKNQVQVETFASSTFSSFLDVLLSCFVFMALTLACFLPPLVSPATVGPPATAAVTLAALAGLLEVASVVLSVRMAFCLEEVMNCTNSLLLVVSGWIPRHVIGAVLISLPSISVLSHLSCSVHFPLQVTMLLCCATILAIIQYCNFCQLSFWMRSVLATATGVILLLVLYSPLHRMNTLSSGGSPSVTPGPDPPPEPLDILIPEAVLAFFLLLLLVWFLNREFEVSYRLHYHGNVEADKHRFKNQTMRDQAEWLLGNIIPIHVAEQLKVTQSYSKNHDSVGVIFASIVNFSEFYEETYEGGKECYRVLNELIGDFDELLRKDEFKSVEKIKTIGATYMAASGLNVQQLAENDNDSPHAHLRALFNFALEMMGVLEDFNKNMLGFGFKLRIGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDSTGVECRVQVSEESHAVLSAMGLEFDYRGTVNVKGKGQMRTYLYPKSGESIFQDKIELGPSDGHSPMASTAIKTPGSAAQAAGLSPYSSHLSTDPPQPQSAVGPSITGPEHDHAQFTEVREEGYRDAPPPPGQPTAPDPHSLPRSELVPEPSCAEVESHVEPSPLTLQEQKEEWEANELTKLNEEFYARF

Target ORF information:

RefSeq Version	XM_011612524.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes adenylate cyclase type 9-like (LOC101074204), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612524.1

ORF Insert Sequence:

ATGGCCTCTC TGCAACACCA CCAGCCTCTC ATGCATGGCA CAGAAGTGAG CTGTGACTCC 
AGCGGGGATG GCGCCGTCAC CGTGCAGATC ACCAACAGCG TGCCGCACAT GCATCGCGAG 
CCCCAGGCTG GAGCGGGAGG AGCGAAAACG AACCCCAACC TGCACAAATA CAGCATCTCT 
TCCAGCTGCA GCTCGGCGGA CTCCGGAACG GCCGCGGCGA CCACGTCCAG GGTGCGTCGG 
AGAGTGCCGC CATTGTTTGA GCGCTCCGCC GCTCAGTGCT GGGACCCCAA ATTTGATTCG 
CCCATTCTGG AAGAGGCTTG CCAGGAGAGG TGCTTTCCTC AGACGCAGCG GCGCTTTCGT 
TACGTTCTGT CGTACCTGGC AGTGGCAGCT GTTCTTTGGG GGGTTTACTT TGGAGTCAAT 
AGTGACCGCT GCAGCCCTGT GGCCTTTTTA GCCCCAACAG CATCGTTTTT AGTGCTGCTC 
ATGCTCCTCC TGTTGTTCAC CTTTACCCAA CCCTACACCT GGCTGTACAA CCAAACGTCC 
TTGTTTCTAA TCGCCGTTAC CTTCGCCATC ACTCTGGCGC CACAGATACA GACTGCTGGC 
TACATTCAGC TGGGGTGGGC TGCTGGACTG AACGCCTCGT TCCTCTCACC CGACAGAGTT 
GCCCCGCTCC CAACCCCTTG CATCTCCCCT GTTGGCACCT TTTCGTTGTG CATGGAGGTG 
CTGTTGTTGC TGTACAGCGT CCTCCACGTC CGCCTGTATG CGTCAGTGAT GCTGGGGTTC 
TTATATTCTG TTTTGTTTGA GGCGTTAGAA TGGCTGCCAT TGCTTCAGAA GAGCTATGAC 
ACTACGGGGT GGCAGACAGA ATCGGTCGGC TCGGGTTCTG ATTGGGAAGG ACACACCCTC 
CGCTGGCTGG GCCCAGCCAA AGCTCTGCTC CACTTGTGCG CCCATGTGAT TGGCATCCAC 
CTGTTCATCA TGTCAGAGGT ACGCTCCCGC AGCACCTTCC TCAAAGTGGG CCAGGCCATC 
ATGCACGGCA AAGACCTGGA GGTCGAAAAG GCCTTGAAGG AACGTATGAT TCACTCCGTA 
ATGCCACGGC GGGTTGCGGA CGATCTCCTG AAGCAGGGGG ACGACGAGGG CTGCGGCGAG 
AGTTCCGGCA AGCGCTACTC TTCCGGCGCC TGCTCCGCCT CTGCAGTCTC GGTGGGCGGC 
GGGGGAGGCG GCGGATTGTC GCAAGCCCAG AGCGTCACAA ACGCTAAGCA CGGCCACAAC 
AATCACAAAA GCAAAAAAAC CTCCATTCCC CGCGGACAGA TCATCTTTCG GCCCTTCAAC 
ATGAAGCGCA TGGAGCCTGT CAGCATCCTC TTCGCCGATA TCGTGGGTTT CACCAAAATG 
TCGGCCAATA AGTCGGCGCC GGCCCTGGTA GGCCTTCTCA ATGATCTCTT CGGACGTTTT 
GACCGGCTGT GCGAGCTTAC TGGTTGCGAG AAGATCAGCA CTCTGGGAGA CTGCTATTAT 
TGTGTGGCCG GCTGTCCCGA GCCCAGCCCG GACCACGCCT ACTGCTGTGT GGAAATGGGC 
TTGGGGATGA TCCAGGCCAT CGAACAGTTC TGCCAGGAGA CGCGCGAGAC CGTCAGCATG 
CGCGTTGGGG TCCATACCGG CACCGTCCTG TGCGGGATCC TGGGAATGAA GCGTTTTAAG 
TTTGATGTCT GGTCAAATGA TGTCAATCTG GCCAATCTGA TGGAGCAGCT GGGTGTGGCG 
GGCAAGATCC ACCTGTCAGA AGCAACTGCC CAGTTCCTGG GTGACCGTTA CCAGAGGGAA 
GACGGGCGAG TGTTGGAGAG AGCGGGGCAG AGCGCGGTGG AGAAACTCAA GGGGATGAAG 
ACCTACCTGA TCTCAGGAAA GAAGCTGGAG CGCGACGTCC CGTGTGGCTG TTCTGACTTT 
GTCAGCCGGT CGCCACACCC ACAGCCCCGC ACGCCAGACC TGATTTCTAG AGGAGTGCAG 
GCAGCTCAGG TGCTACTGAC CCACACAGGC CCTGAAAAGG TGGGGTCTCC CTGTGTTTCC 
CACTGTGTGA GCAAGTCAGC AGCAGAACCG GGTGATGTCG TAACTGTGCT CAACAGCTGT 
CAAGAAGAGA ATAAGACCAA CACCTCCAGG CTCATCCCTG GTCGCAGATA CAGAGCTGCC 
AACGGTCTCC TCAGCCCACC GGTAGAAGAC CCCGTGAGAG CCAGCCAGAG CTCCCTGTGT 
GATATTCTCC AGGAGAAAGA CAAAAAGACC AGTACAAGCA CAGGGACCAG CGCCAGCTTA 
GGCCTTGCTG GTGGAACGGG GACAGCCGGT ACTGGAATGG ACCACTCTGC TCTGATCCAC 
CTGCGAGCGA AAAACTTCAG AGAGAAGAGT GACGCTCACT TTGTGGACGT TATCAGGGAG 
GATAGTCTGA AGGACTACTT TTTCAAACCG CCCATCAACA AGATCAGCCT TAATTTCCTA 
GAGAGACCTC TGGAAAAGGC CTATCACAGC AGCTACAAAG AGGAGGTGAA GAACCAAGTC 
CAGGTAGAGA CATTTGCAAG CTCTACCTTC AGCTCTTTCC TGGACGTTCT CCTCAGCTGT 
TTCGTATTCA TGGCCCTGAC ACTGGCCTGT TTCCTTCCTC CCCTGGTGAG CCCGGCCACC 
GTAGGCCCAC CAGCCACCGC TGCCGTCACC CTGGCTGCCC TGGCCGGGCT GTTGGAAGTG 
GCATCTGTGG TGCTGTCTGT ACGTATGGCC TTCTGTCTGG AGGAGGTAAT GAACTGTACC 
AACTCCCTTC TCCTGGTGGT CTCTGGATGG ATCCCCCGTC ATGTGATTGG AGCAGTGTTG 
ATCTCTCTCC CTTCTATCTC TGTCCTGTCC CACCTCTCCT GCAGTGTCCA CTTCCCTCTA 
CAGGTGACCA TGTTGCTCTG CTGTGCAACC ATCCTCGCCA TCATCCAGTA TTGTAACTTC 
TGTCAGCTCA GTTTTTGGAT GCGTTCAGTC TTGGCCACAG CCACAGGGGT CATCCTGCTG 
CTGGTTCTGT ACAGCCCCTT GCACAGGATG AACACATTGT CGTCTGGTGG TTCACCATCA 
GTCACCCCTG GCCCTGACCC TCCTCCAGAA CCTCTGGACA TTCTAATCCC AGAAGCTGTT 
CTTGCCTTCT TCCTTCTCTT ACTCCTGGTG TGGTTCCTCA ACCGTGAGTT TGAAGTCAGC 
TACCGTCTTC ATTACCATGG CAACGTAGAG GCTGACAAAC ACCGCTTTAA GAACCAGACG 
ATGCGAGACC AGGCTGAGTG GTTACTGGGC AACATCATCC CCATCCATGT GGCCGAACAG 
CTCAAGGTGA CCCAGAGCTA CTCAAAGAAC CACGACAGTG TGGGCGTGAT CTTTGCCAGC 
ATCGTAAACT TCAGCGAGTT TTATGAGGAG ACCTACGAAG GTGGTAAGGA GTGCTACCGT 
GTCCTCAACG AGCTCATCGG AGACTTTGAC GAGCTGCTGC GCAAAGATGA ATTCAAAAGC 
GTGGAAAAGA TTAAAACGAT AGGAGCAACC TACATGGCTG CGTCTGGGCT GAATGTCCAG 
CAGTTGGCTG AGAATGACAA TGACTCTCCA CACGCTCACC TCAGAGCCCT CTTTAACTTT 
GCCCTGGAGA TGATGGGAGT CCTGGAGGAT TTTAATAAGA ACATGCTGGG TTTCGGGTTC 
AAACTCCGTA TTGGATTTAA CCACGGGCCA CTGACAGCAG GGGTGATCGG CACCACTAAG 
TTGCTCTATG ACATCTGGGG AGACACTGTC AACATTGCGA GCCGCATGGA CTCTACAGGT 
GTGGAGTGTC GGGTGCAGGT GAGCGAGGAG AGCCATGCTG TGCTCAGCGC CATGGGATTG 
GAGTTTGACT ATAGAGGTAC AGTGAATGTT AAAGGCAAAG GTCAAATGAG GACCTATCTC 
TACCCCAAAA GTGGGGAAAG CATATTTCAA GATAAAATAG AACTGGGTCC CAGTGACGGA 
CATTCACCCA TGGCATCAAC AGCCATTAAG ACTCCAGGAT CAGCTGCTCA AGCAGCAGGT 
CTTTCTCCTT ATTCCTCCCA CCTTTCCACA GACCCTCCCC AACCACAAAG TGCTGTAGGG 
CCTTCAATTA CAGGACCTGA GCATGACCAT GCTCAGTTCA CAGAGGTGAG GGAGGAAGGG 
TATAGGGACG CTCCACCACC CCCGGGGCAG CCCACGGCCC CAGACCCTCA CTCCCTGCCC 
CGCTCTGAAC TCGTCCCAGA ACCTAGTTGT GCAGAAGTGG AATCCCATGT GGAGCCTTCT 
CCTCTCACGC TCCAAGAACA GAAAGAAGAG TGGGAGGCTA ACGAGCTAAC CAAACTCAAC 
GAGGAGTTTT ACGCTCGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC101074204 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム