LOC101065799 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101065799
Entrez Gene ID	101065799
Full Name	rho GTPase-activating protein 20-like
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003962219.3	XP_003962268.2	rho GTPase-activating protein 20-like
XM_003962219.2	XP_003962268.1	rho GTPase-activating protein 20-like

Pathways from BioSystems
Gene Ontology

The following LOC101065799 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101065799 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo42058	XM_003962219.3 Latest version!	Takifugu rubripes rho GTPase-activating protein 20-like (LOC101065799), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo01255	XM_003962219.2 Latest version!	Takifugu rubripes rho GTPase-activating protein 20-like (LOC101065799), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101065799 ( XM_003962219.3 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003962268.2 Takifugu rubripes rho GTPase-activating protein 20-like (LOC101065799), mRNA.

Price & Availability↑

CloneID	OTo42058
Clone ID Related Accession (Same CDS sequence)	XM_003962219.3
Accession Version	XM_003962219.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	rho GTPase-activating protein 20-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003962219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCA TGTCGCCGCA GCAGGGGACC ATGGGACAGA ACAGGTCCGA CTCGCTGACG 
GGGGAGATCA AGTCGCTGCC CGAAAACAAA AAGAAGATGA AGACCTTGGC TCAGAGGCGG 
CAGTCGGCTC CCTCACTGGT CATCAGCAAA GCACTCACCA GATCACGGAG CACGTCCAGG 
GAAAGCTGCC TGACACCAGT CAGCCCAGAA TCATGTCCAC TCGTCCAGGC CTTCCTCGCC 
GAGTGTCCAG CCCGTCTGTT CTTGGATCAC GCCCAAGCAC AGCTGAAGAC AGGCCTGCAG 
ACCCAGGAGA GACACCTCTT CCTCTTTAAT GACACCCTCC TGGTCGCCAA GGCCAAATCT 
CCCACCCATT TTAAGGTGAA GGCCCAAGCC CGAGTGTGTG AGATGTGGGT GTCCCACTGC 
ATAGAGGAAG TGTGTGAGGG AAGCACAAAC CCAGAGAAGA GCTTCGTCGT GGGCTGGCCC 
ACCTGCAACT TCGTGGCCAC CTTCAGCTCG GAGGAGCACA AGAACAAGTG GTTGGAGCTC 
ATCAAAAGCG GGATCCTTGA GGGAAAAGAC AGGGACGACC CCAAGACGGT CCCCCTGAAG 
ATCTTCGCCA AGGACGTTGG AAACTGCGCA TATGCCAAGA CGCTCGCGGT CAGCAACGCC 
GACAGCACCG ATGATGTCAT CCGCATGGCC CTGCTGCAGT TTGGCATATC GGGCTGTGTG 
AAGGACCACC GGTTGTGGGT GAGCTCCAGT AAGGATGAGC CTCCGTATCC TCTCATTGGC 
CACGAGTTTC CCTTCAGTGT CAAGATGAGC CACATTCGGA ATGGCGGGAG CAGCGCTGCG 
GGTCTGGGAG GAGGAGCCGT CAGGGATCCC ACCAGTCCCA CAGACTGTCC GGGGGTCCTG 
CTGCTGGACC AGTGCCTTCC ACCTGACAAC CAGTGCCAGT TCATCCTCAA ACCCAACAAG 
GCTGTCCCGG GACAGGTGCA ACTTATGGAA CCTGCTCAGC AAAAGACCTT CAAGAGGAAG 
CGATCCCTGA TCAACTGGCC CTTCTGGAAG GGCTCCAACC CCCAGCTGGA CAGCGTGCCC 
CTGTCCCCCA CCTCGCTCAG CCCCACTCAG GGGCGGCTGT TCGGACGGTC GCTAAGCTCC 
ATCTGCTCTC CAGAACACGG CCTGCCCAAG CCCGTCATGG ACATGCTGGT GCATCTGTAC 
CTGGAGGGGC CGTACACCAG GGGGGTCTTC AGGAGGTCGG CTGGAGCCAA AGCCTGCCGG 
GAACTCCGGG AGCAACTGGA CAGAGGAACC GAGGATCCAG AGATCACACA TCAATCTGTG 
TTCGTCATCG CTGCTGTCCT GAAGGACTTC CTGCGGAATA TCCCGGGCAG CCTGCTGTGC 
GTGGATCTGT ATGACCGGTG GATGGATGCG ATGGGAACTG AGGGGGCAGA GGAGAAAATG 
CAATCCATCC ACAGGTTGCT CCACCTCCTG CCTGGTGAAA ACCTCCTCCT GCTGCGGCAC 
GTTATCGCCG TGTTGCACTG TATCCAAGGC AACGCTCAAG ACAACCAGAT GAATGCCTTC 
AACTTGTCCG TCTGCATCGC GCCCAGCATG CTTTGGTCCC AGGCACCAAG CACCCCTGAG 
ATGGAGGGGG AGGGCACCAA AAAGGTGTGT GAGCTGGTGC GACTCCTCAT AGAGAACTGC 
TGCGGCGCTC TGGGAGACGA CGTGACGGCG CTGTTCAGAA GCTTCAGCCA GAAGAGCAGC 
AGCAGCGACC ACGGATCAGA TGTGTCATCC TTCCAGATGA ATGACTCTTC CTACGACAGC 
CTAGAGAACG AGCTGAACGA CGACCCTGAG TCGCCCTACC AGGAGCAGCT GCCATTGCGC 
GACAAGGACA AACCAGACAG TCGCAGCCGG GACTCCGTCA TCACCCTCAG CGACTGTGAC 
CCTGATCCTG ACCCGGAGGC TGAGCTCCTA TTGCAGCTCC CCCAACTGGC CAGGCCGAGG 
AGGTTCAGCC CCGCGGTGCG ACAGCCGCGC CCCCGCCAGG CCAACGGGCA GTCCCAGGGC 
CCCAGAAGGC TGCGGAGGAG TTCGGAACCT GCCTTAACGC TGGAAAGCAT GGCCCCCTCG 
TGCACAGTGA CGGCACTAGC TAACACACAG CGCCTGCCTG TGCGAAAGGC GAGCTACGAT 
GCAGCCATGG AAGGGAAGGG GGAGGAGGAC AGGGTGTTTC TGGAGCAGAG GCTGAGCGGT 
CTTCAGCTGA AGGAGGAGGA AGAGGAGTCG AATAAGAAGG GTGGCGCCAA AGTCCAAAAC 
GGTGGACGGA GGAAGGTGAA GCACACCCCT CCGCCGCCGT TGCGTCTGGA CGCCAGCTGT 
TCCAGCCTGT CGTCACCTGC AACCTCGCCC ACAGGCTCCT CCCTCAGTTC TTTAGACTCC 
GCCTTCTCAC AGTACTCTAC TGACTACACC ACCAATGGTG CCGTTCCACT GGCAGAGCCT 
GCACCCCTCT CACCAGGGCG GCCAGAGATG TCACCCAAAG GCTCCCCACC TCAGAGGGAG 
GCCTGGACAC AACCAAGCCA ACCCCCCTCC AATTCTCACG GACTCCATCC CAACACGTGG 
CTCAGTAAGG ACCGCTGCCT GTCACTACGG CAGCCCGAGG AGGACACGCC CGCGGCGAAC 
AGAAAGACTC CAACCAGTAA TGGTTACTCC ACCCTCCAGG AGGCCAAAAG CAGAACCTGC 
CAAAGGAGAT CCAGCAGCCC TCCGTCCTAC CAGCAGGCTC TGCTGCAGCT GCAGCGCAAC 
CGCTCGCCAT TCTATCGAGG CACAGAGAAA CCTCTGACGG TCAAAGAACT GAGGCAGCTC 
CACGACCAGA CTCCCAGCTA CAACCCTAAA CCACAAACAG GAAGTGATGT CACCCAGAGG 
TTGGGAACAA ATGATCATGT GCAGCCCCCA CAGGGAGTTT TCTATGGACA AAGTGGAACC 
ACTTTAGTCC TCCAAAGGCA AAAGTCCCAC CCTCTGACTC AGGACACGAA AGGACAAAAA 
GCAGTAGGGA CCCTTAATCT TCCGCGAAGG GCATCGGAAC CTCCCGTGGT CAACTTAAAC 
CTCACCTCCA GCCTCACGCG TGACAGAGCT CACGCTTCAA AGGGTACAGG AGGATTGAGA 
GTGTCTGAGG TGGAGAGCAG CCACAGCGAG CCACGCTTCT GTCTGTCTCC ATCCGCCACC 
AGGGCAGTGA GGGACTACTT TTCCTCCCAG GGCCAGGAGG ATGCAGACGG CTGCCTGAGG 
AGGAGTCAGG AGGTGGCGCT GGCTATTGTA CAGAGAAAGA GAGAATGGCA GAGCCGGCGG 
TGCAGTGACC CACGAGTGGA TGAGTTTGAG AAACTGTTTT TTGCAGAAGA ATCGTACGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003962268.2
CDS	211..3573
Translation	METMSPQQGTMGQNRSDSLTGEIKSLPENKKKMKTLAQRRQSAPSLVISKALTRSRSTSRESCLTPVSPESCPLVQAFLAECPARLFLDHAQAQLKTGLQTQERHLFLFNDTLLVAKAKSPTHFKVKAQARVCEMWVSHCIEEVCEGSTNPEKSFVVGWPTCNFVATFSSEEHKNKWLELIKSGILEGKDRDDPKTVPLKIFAKDVGNCAYAKTLAVSNADSTDDVIRMALLQFGISGCVKDHRLWVSSSKDEPPYPLIGHEFPFSVKMSHIRNGGSSAAGLGGGAVRDPTSPTDCPGVLLLDQCLPPDNQCQFILKPNKAVPGQVQLMEPAQQKTFKRKRSLINWPFWKGSNPQLDSVPLSPTSLSPTQGRLFGRSLSSICSPEHGLPKPVMDMLVHLYLEGPYTRGVFRRSAGAKACRELREQLDRGTEDPEITHQSVFVIAAVLKDFLRNIPGSLLCVDLYDRWMDAMGTEGAEEKMQSIHRLLHLLPGENLLLLRHVIAVLHCIQGNAQDNQMNAFNLSVCIAPSMLWSQAPSTPEMEGEGTKKVCELVRLLIENCCGALGDDVTALFRSFSQKSSSSDHGSDVSSFQMNDSSYDSLENELNDDPESPYQEQLPLRDKDKPDSRSRDSVITLSDCDPDPDPEAELLLQLPQLARPRRFSPAVRQPRPRQANGQSQGPRRLRRSSEPALTLESMAPSCTVTALANTQRLPVRKASYDAAMEGKGEEDRVFLEQRLSGLQLKEEEEESNKKGGAKVQNGGRRKVKHTPPPPLRLDASCSSLSSPATSPTGSSLSSLDSAFSQYSTDYTTNGAVPLAEPAPLSPGRPEMSPKGSPPQREAWTQPSQPPSNSHGLHPNTWLSKDRCLSLRQPEEDTPAANRKTPTSNGYSTLQEAKSRTCQRRSSSPPSYQQALLQLQRNRSPFYRGTEKPLTVKELRQLHDQTPSYNPKPQTGSDVTQRLGTNDHVQPPQGVFYGQSGTTLVLQRQKSHPLTQDTKGQKAVGTLNLPRRASEPPVVNLNLTSSLTRDRAHASKGTGGLRVSEVESSHSEPRFCLSPSATRAVRDYFSSQGQEDADGCLRRSQEVALAIVQRKREWQSRRCSDPRVDEFEKLFFAEESYV

Target ORF information:

RefSeq Version	XM_003962219.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes rho GTPase-activating protein 20-like (LOC101065799), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003962219.3

ORF Insert Sequence:

ATGGAAACCA TGTCGCCGCA GCAGGGGACC ATGGGACAGA ACAGGTCCGA CTCGCTGACG 
GGGGAGATCA AGTCGCTGCC CGAAAACAAA AAGAAGATGA AGACCTTGGC TCAGAGGCGG 
CAGTCGGCTC CCTCACTGGT CATCAGCAAA GCACTCACCA GATCACGGAG CACGTCCAGG 
GAAAGCTGCC TGACACCAGT CAGCCCAGAA TCATGTCCAC TCGTCCAGGC CTTCCTCGCC 
GAGTGTCCAG CCCGTCTGTT CTTGGATCAC GCCCAAGCAC AGCTGAAGAC AGGCCTGCAG 
ACCCAGGAGA GACACCTCTT CCTCTTTAAT GACACCCTCC TGGTCGCCAA GGCCAAATCT 
CCCACCCATT TTAAGGTGAA GGCCCAAGCC CGAGTGTGTG AGATGTGGGT GTCCCACTGC 
ATAGAGGAAG TGTGTGAGGG AAGCACAAAC CCAGAGAAGA GCTTCGTCGT GGGCTGGCCC 
ACCTGCAACT TCGTGGCCAC CTTCAGCTCG GAGGAGCACA AGAACAAGTG GTTGGAGCTC 
ATCAAAAGCG GGATCCTTGA GGGAAAAGAC AGGGACGACC CCAAGACGGT CCCCCTGAAG 
ATCTTCGCCA AGGACGTTGG AAACTGCGCA TATGCCAAGA CGCTCGCGGT CAGCAACGCC 
GACAGCACCG ATGATGTCAT CCGCATGGCC CTGCTGCAGT TTGGCATATC GGGCTGTGTG 
AAGGACCACC GGTTGTGGGT GAGCTCCAGT AAGGATGAGC CTCCGTATCC TCTCATTGGC 
CACGAGTTTC CCTTCAGTGT CAAGATGAGC CACATTCGGA ATGGCGGGAG CAGCGCTGCG 
GGTCTGGGAG GAGGAGCCGT CAGGGATCCC ACCAGTCCCA CAGACTGTCC GGGGGTCCTG 
CTGCTGGACC AGTGCCTTCC ACCTGACAAC CAGTGCCAGT TCATCCTCAA ACCCAACAAG 
GCTGTCCCGG GACAGGTGCA ACTTATGGAA CCTGCTCAGC AAAAGACCTT CAAGAGGAAG 
CGATCCCTGA TCAACTGGCC CTTCTGGAAG GGCTCCAACC CCCAGCTGGA CAGCGTGCCC 
CTGTCCCCCA CCTCGCTCAG CCCCACTCAG GGGCGGCTGT TCGGACGGTC GCTAAGCTCC 
ATCTGCTCTC CAGAACACGG CCTGCCCAAG CCCGTCATGG ACATGCTGGT GCATCTGTAC 
CTGGAGGGGC CGTACACCAG GGGGGTCTTC AGGAGGTCGG CTGGAGCCAA AGCCTGCCGG 
GAACTCCGGG AGCAACTGGA CAGAGGAACC GAGGATCCAG AGATCACACA TCAATCTGTG 
TTCGTCATCG CTGCTGTCCT GAAGGACTTC CTGCGGAATA TCCCGGGCAG CCTGCTGTGC 
GTGGATCTGT ATGACCGGTG GATGGATGCG ATGGGAACTG AGGGGGCAGA GGAGAAAATG 
CAATCCATCC ACAGGTTGCT CCACCTCCTG CCTGGTGAAA ACCTCCTCCT GCTGCGGCAC 
GTTATCGCCG TGTTGCACTG TATCCAAGGC AACGCTCAAG ACAACCAGAT GAATGCCTTC 
AACTTGTCCG TCTGCATCGC GCCCAGCATG CTTTGGTCCC AGGCACCAAG CACCCCTGAG 
ATGGAGGGGG AGGGCACCAA AAAGGTGTGT GAGCTGGTGC GACTCCTCAT AGAGAACTGC 
TGCGGCGCTC TGGGAGACGA CGTGACGGCG CTGTTCAGAA GCTTCAGCCA GAAGAGCAGC 
AGCAGCGACC ACGGATCAGA TGTGTCATCC TTCCAGATGA ATGACTCTTC CTACGACAGC 
CTAGAGAACG AGCTGAACGA CGACCCTGAG TCGCCCTACC AGGAGCAGCT GCCATTGCGC 
GACAAGGACA AACCAGACAG TCGCAGCCGG GACTCCGTCA TCACCCTCAG CGACTGTGAC 
CCTGATCCTG ACCCGGAGGC TGAGCTCCTA TTGCAGCTCC CCCAACTGGC CAGGCCGAGG 
AGGTTCAGCC CCGCGGTGCG ACAGCCGCGC CCCCGCCAGG CCAACGGGCA GTCCCAGGGC 
CCCAGAAGGC TGCGGAGGAG TTCGGAACCT GCCTTAACGC TGGAAAGCAT GGCCCCCTCG 
TGCACAGTGA CGGCACTAGC TAACACACAG CGCCTGCCTG TGCGAAAGGC GAGCTACGAT 
GCAGCCATGG AAGGGAAGGG GGAGGAGGAC AGGGTGTTTC TGGAGCAGAG GCTGAGCGGT 
CTTCAGCTGA AGGAGGAGGA AGAGGAGTCG AATAAGAAGG GTGGCGCCAA AGTCCAAAAC 
GGTGGACGGA GGAAGGTGAA GCACACCCCT CCGCCGCCGT TGCGTCTGGA CGCCAGCTGT 
TCCAGCCTGT CGTCACCTGC AACCTCGCCC ACAGGCTCCT CCCTCAGTTC TTTAGACTCC 
GCCTTCTCAC AGTACTCTAC TGACTACACC ACCAATGGTG CCGTTCCACT GGCAGAGCCT 
GCACCCCTCT CACCAGGGCG GCCAGAGATG TCACCCAAAG GCTCCCCACC TCAGAGGGAG 
GCCTGGACAC AACCAAGCCA ACCCCCCTCC AATTCTCACG GACTCCATCC CAACACGTGG 
CTCAGTAAGG ACCGCTGCCT GTCACTACGG CAGCCCGAGG AGGACACGCC CGCGGCGAAC 
AGAAAGACTC CAACCAGTAA TGGTTACTCC ACCCTCCAGG AGGCCAAAAG CAGAACCTGC 
CAAAGGAGAT CCAGCAGCCC TCCGTCCTAC CAGCAGGCTC TGCTGCAGCT GCAGCGCAAC 
CGCTCGCCAT TCTATCGAGG CACAGAGAAA CCTCTGACGG TCAAAGAACT GAGGCAGCTC 
CACGACCAGA CTCCCAGCTA CAACCCTAAA CCACAAACAG GAAGTGATGT CACCCAGAGG 
TTGGGAACAA ATGATCATGT GCAGCCCCCA CAGGGAGTTT TCTATGGACA AAGTGGAACC 
ACTTTAGTCC TCCAAAGGCA AAAGTCCCAC CCTCTGACTC AGGACACGAA AGGACAAAAA 
GCAGTAGGGA CCCTTAATCT TCCGCGAAGG GCATCGGAAC CTCCCGTGGT CAACTTAAAC 
CTCACCTCCA GCCTCACGCG TGACAGAGCT CACGCTTCAA AGGGTACAGG AGGATTGAGA 
GTGTCTGAGG TGGAGAGCAG CCACAGCGAG CCACGCTTCT GTCTGTCTCC ATCCGCCACC 
AGGGCAGTGA GGGACTACTT TTCCTCCCAG GGCCAGGAGG ATGCAGACGG CTGCCTGAGG 
AGGAGTCAGG AGGTGGCGCT GGCTATTGTA CAGAGAAAGA GAGAATGGCA GAGCCGGCGG 
TGCAGTGACC CACGAGTGGA TGAGTTTGAG AAACTGTTTT TTGCAGAAGA ATCGTACGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101065799 ( XM_003962219.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003962268.1 Takifugu rubripes rho GTPase-activating protein 20-like (LOC101065799), mRNA.

Price & Availability↑

CloneID	OTo01255
Clone ID Related Accession (Same CDS sequence)	XM_003962219.2
Accession Version	XM_003962219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	rho GTPase-activating protein 20-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070914.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003962219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCA TGTCGCCGCA GCAGGGGACC ATGGGACAGA ACAGGTCCGA CTCGCTGACG 
GGGGAGATCA AGTCGCTGCC CGAAAACAAA AAGAAGATGA AGACCTTGGC TCAGAGGCGG 
CAGTCGGCTC CCTCACTGGT CATCAGCAAA GCACTCACCA GATCACGGAG CACGTCCAGG 
GAAAGCTGCC TGACACCAGT CAGCCCAGAA TCATGTCCAC TCGTCCAGGC CTTCCTCGCC 
GAGTGTCCAG CCCGTCTGTT CTTGGATCAC GCCCAAGCAC AGCTGAAGAC AGGCCTGCAG 
ACCCAGGAGA GACACCTCTT CCTCTTTAAT GACACCCTCC TGGTCGCCAA GGCCAAATCT 
CCCACCCATT TTAAGGTGAA GGCCCAAGCC CGAGTGTGTG AGATGTGGGT GTCCCACTGC 
ATAGAGGAAG TGTGTGAGGG AAGCACAAAC CCAGAGAAGA GCTTCGTCGT GGGCTGGCCC 
ACCTGCAACT TCGTGGCCAC CTTCAGCTCG GAGGAGCACA AGAACAAGTG GTTGGAGCTC 
ATCAAAAGCG GGATCCTTGA GGGAAAAGAC AGGGACGACC CCAAGACGGT CCCCCTGAAG 
ATCTTCGCCA AGGACGTTGG AAACTGCGCA TATGCCAAGA CGCTCGCGGT CAGCAACGCC 
GACAGCACCG ATGATGTCAT CCGCATGGCC CTGCTGCAGT TTGGCATATC GGGCTGTGTG 
AAGGACCACC GGTTGTGGGT GAGCTCCAGT AAGGATGAGC CTCCGTATCC TCTCATTGGC 
CACGAGTTTC CCTTCAGTGT CAAGATGAGC CACATTCGGA ATGGCGGGAG CAGCGCTGCG 
GGTCTGGGAG GAGGAGCCGT CAGGGATCCC ACCAGTCCCA CAGACTGTCC GGGTGTCCTG 
CTGCTGGACC AGTGTCTCCC ACCTGACAAC CAGTGCCAGT TCATCCTCAA ACCCAACAAG 
GCTGTCCCGG GACAGGTGCA ACTTATGGAA CCTGCTCAGC AAAAGACCTT CAAGAGGAAG 
CGATCCCTGA TCAACTGGCC CTTCTGGAAG GGCTCCAACC CCCAGCTGGA CAGCGTGCCC 
CTGTCCCCCA CCTCGCTCGG CCCCACTCAG GGGCGGCTGT TCGGACGGTC GCTAAGCTCC 
ATCTGCTCTC CAGAACACGG CCTGCCCAAG CCCGTCATGG ACATGCTGGT GCATCTGTAC 
CTGGAGGGGC CGTACACCAG GGGGGTCTTC AGGAGGTCGG CTGGAGCCAA AGCCTGCCGG 
GAACTCCGGG AGCAACTGGA CAGAGGAACC GAGGATCCAG AGATCACACA TCAATCTGTG 
TTCGTCATCG CTGCTGTCCT GAAGGACTTC CTGCGGAATA TCCCGGGCAG CCTGCTGTGC 
GTGGATCTGT ATGACCGGTG GATGGATGCG ATGGGAACTG AGGGGGCAGA GGAGAAAATA 
CAATCCATCC ACAGGTTGCT CCACCTCCTG CCTGGTGAAA ACCTCCTCCT GCTGCGGCAC 
GTTATCGCCG TGTTGCACTG TATCCAAGGC AACGCTCAAG ACAACCAGAT GAATGCCTTC 
AACTTGTCCG TCTGCATCGC GCCCAGCATG CTTTGGTCCC AGGCACCAAG CACCCCTGAG 
ATGGAGGGGG AGGGCACCAA AAAGGTGTGT GAGCTGGTGC GACTCCTCAT AGAGAACTGC 
TGCGGCGCTC TGGGAGACGA CGTGACGGCG CTGTTCAGAA GCTTCAGCCA GAAGAGCAGC 
AGCAGCGACC ACGGATCAGA TGTGTCGTCC TTCCAGATGA ATGACTCTTC CTACGACAGC 
CTAGAGAACG AGCTGAACGA CGACCCTGAG TCGCCCTACC AGGAGCAGCT GCCATTGCGC 
GACAAGGACA AACCAGACAG TCGCAGCCGT GACTCCGTCA TCACCCTCAG CGACTGTGAC 
CCTGATCCTG ACCCGGAGGC TGAGCTCCTA TTGCAGCTCC CCCAACTGGC CAGGCCGAGG 
AGGTTCAGCC CCGCGGTGCG ACAGCCGCGC CCCCGCCAGG CCAACGGGCA GTCCCAGGGC 
CCCAGAAGGC TGCGGAGGAG TTCGGAACCT GCCTTAACGC TGGAAAGCAT GGCCCCCTCG 
TGCACAGTGA CGGCACTAGC TAACACACAG CGCCTGCCTG TGCGAAAGGC GAGCTACGAT 
GCAGCCATGG AAGGGAAGGG GGAGGAGGAC AGGGTGTTTC TGGAGCAGAG GCTGAGCGGT 
CTTCAGCTGA AGGAGGAGGA AGAGGAGTCG AATAAGAAGG GTGGCGCCAA AGTCCAAAAC 
GGTGGACGGA GGAAGGTGAA GCACACCCCT CCGCCGCCTT TGCGTCTGGA CGCCAGCTGT 
TCCAGCCTGT CGTCACCTGC AACCTCGCCC ACAGGCTCCT CCCTCAGTTC TTTAGACTCC 
GCCTTCTCAC AGTACTCTAC TGACTACACC ACCAATGGTG CCATTCCACT GGCAGAGCCT 
GCACCCCTGT CACCAGGGCG GCCAGAGATG TCACCCAAAG GCTCCCCACC TCAGAGGGAG 
GCCTGGACAC AACCAAGCCA ACCCCCCTCC AATTCTCACG GACTCCATCC CAACACGTGG 
CTCAGTAAGG ACCGCTGCCT GTCACTACGG CAGCCCGAGG AGGACACGCC CGCGGCGAAC 
AGAAAGACTC CAACCAGTAA TGGTTACTCC ACCCTCCAGG AGGCCAAAAG CAGAACCTGC 
CAAAGGAGAT CCAGCAGCCC TCCGTCCTAC CAGCAGGCTC TGCTGCAGCT GCAGCGCAAC 
CGCTCGCCAT TCTATCGAGG CACAGAGAAA CCTCTGACGG TCAAAGAACT GAGGCAGCTC 
CACGACCAGA CTCCCAGCTA CAACCCTAAA CCACAAACAG GAAGTGATGT CACTCCGAGG 
TTGGGAACAA ATGATCATGT GCAGCCCCCA CAGGGAGTTT TCTATGGACA AAGTGGAACC 
ACTTTAGTCC TCCAAAGGCA AAAGTCCCAC CCTCTGACTC AGGACACGAA AGGACAAAAA 
GCAGTAGGGA CCCTTAATCT TCCGCGAAGG GCATCGGAAC CTCCCGTGGT CAACTTAAAC 
CTCACCTCCA GCCTCACGCG TGACAGAGCT CACGCTTCAA AGGGTACAGG AGGATTGAGA 
GTGTCTGAGG TGGAGAGCAG CCACAGCGAG CCACGCTTCT GTCTGTCTCC ATCCGCCACC 
AGGGCAGTGA GGGACTACTT TTCCTCCCAG GGCCAGGAGG ATGCAGACGG CTGCCTGAGG 
AGGAGTCAGG AGGTGGCGCT GGCTATTGTA CAGAGAAAGA GAGAATGGCA GAGCCGGCGG 
TGCAGTGACC CACGAGTGGA TGAGTTTGAG AAACTGTTTT TTGCAGAAGA ATCGTACGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003962268.1
CDS	203..3565
Translation	METMSPQQGTMGQNRSDSLTGEIKSLPENKKKMKTLAQRRQSAPSLVISKALTRSRSTSRESCLTPVSPESCPLVQAFLAECPARLFLDHAQAQLKTGLQTQERHLFLFNDTLLVAKAKSPTHFKVKAQARVCEMWVSHCIEEVCEGSTNPEKSFVVGWPTCNFVATFSSEEHKNKWLELIKSGILEGKDRDDPKTVPLKIFAKDVGNCAYAKTLAVSNADSTDDVIRMALLQFGISGCVKDHRLWVSSSKDEPPYPLIGHEFPFSVKMSHIRNGGSSAAGLGGGAVRDPTSPTDCPGVLLLDQCLPPDNQCQFILKPNKAVPGQVQLMEPAQQKTFKRKRSLINWPFWKGSNPQLDSVPLSPTSLGPTQGRLFGRSLSSICSPEHGLPKPVMDMLVHLYLEGPYTRGVFRRSAGAKACRELREQLDRGTEDPEITHQSVFVIAAVLKDFLRNIPGSLLCVDLYDRWMDAMGTEGAEEKIQSIHRLLHLLPGENLLLLRHVIAVLHCIQGNAQDNQMNAFNLSVCIAPSMLWSQAPSTPEMEGEGTKKVCELVRLLIENCCGALGDDVTALFRSFSQKSSSSDHGSDVSSFQMNDSSYDSLENELNDDPESPYQEQLPLRDKDKPDSRSRDSVITLSDCDPDPDPEAELLLQLPQLARPRRFSPAVRQPRPRQANGQSQGPRRLRRSSEPALTLESMAPSCTVTALANTQRLPVRKASYDAAMEGKGEEDRVFLEQRLSGLQLKEEEEESNKKGGAKVQNGGRRKVKHTPPPPLRLDASCSSLSSPATSPTGSSLSSLDSAFSQYSTDYTTNGAIPLAEPAPLSPGRPEMSPKGSPPQREAWTQPSQPPSNSHGLHPNTWLSKDRCLSLRQPEEDTPAANRKTPTSNGYSTLQEAKSRTCQRRSSSPPSYQQALLQLQRNRSPFYRGTEKPLTVKELRQLHDQTPSYNPKPQTGSDVTPRLGTNDHVQPPQGVFYGQSGTTLVLQRQKSHPLTQDTKGQKAVGTLNLPRRASEPPVVNLNLTSSLTRDRAHASKGTGGLRVSEVESSHSEPRFCLSPSATRAVRDYFSSQGQEDADGCLRRSQEVALAIVQRKREWQSRRCSDPRVDEFEKLFFAEESYV

Target ORF information:

RefSeq Version	XM_003962219.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes rho GTPase-activating protein 20-like (LOC101065799), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003962219.2

ORF Insert Sequence:

ATGGAAACCA TGTCGCCGCA GCAGGGGACC ATGGGACAGA ACAGGTCCGA CTCGCTGACG 
GGGGAGATCA AGTCGCTGCC CGAAAACAAA AAGAAGATGA AGACCTTGGC TCAGAGGCGG 
CAGTCGGCTC CCTCACTGGT CATCAGCAAA GCACTCACCA GATCACGGAG CACGTCCAGG 
GAAAGCTGCC TGACACCAGT CAGCCCAGAA TCATGTCCAC TCGTCCAGGC CTTCCTCGCC 
GAGTGTCCAG CCCGTCTGTT CTTGGATCAC GCCCAAGCAC AGCTGAAGAC AGGCCTGCAG 
ACCCAGGAGA GACACCTCTT CCTCTTTAAT GACACCCTCC TGGTCGCCAA GGCCAAATCT 
CCCACCCATT TTAAGGTGAA GGCCCAAGCC CGAGTGTGTG AGATGTGGGT GTCCCACTGC 
ATAGAGGAAG TGTGTGAGGG AAGCACAAAC CCAGAGAAGA GCTTCGTCGT GGGCTGGCCC 
ACCTGCAACT TCGTGGCCAC CTTCAGCTCG GAGGAGCACA AGAACAAGTG GTTGGAGCTC 
ATCAAAAGCG GGATCCTTGA GGGAAAAGAC AGGGACGACC CCAAGACGGT CCCCCTGAAG 
ATCTTCGCCA AGGACGTTGG AAACTGCGCA TATGCCAAGA CGCTCGCGGT CAGCAACGCC 
GACAGCACCG ATGATGTCAT CCGCATGGCC CTGCTGCAGT TTGGCATATC GGGCTGTGTG 
AAGGACCACC GGTTGTGGGT GAGCTCCAGT AAGGATGAGC CTCCGTATCC TCTCATTGGC 
CACGAGTTTC CCTTCAGTGT CAAGATGAGC CACATTCGGA ATGGCGGGAG CAGCGCTGCG 
GGTCTGGGAG GAGGAGCCGT CAGGGATCCC ACCAGTCCCA CAGACTGTCC GGGTGTCCTG 
CTGCTGGACC AGTGTCTCCC ACCTGACAAC CAGTGCCAGT TCATCCTCAA ACCCAACAAG 
GCTGTCCCGG GACAGGTGCA ACTTATGGAA CCTGCTCAGC AAAAGACCTT CAAGAGGAAG 
CGATCCCTGA TCAACTGGCC CTTCTGGAAG GGCTCCAACC CCCAGCTGGA CAGCGTGCCC 
CTGTCCCCCA CCTCGCTCGG CCCCACTCAG GGGCGGCTGT TCGGACGGTC GCTAAGCTCC 
ATCTGCTCTC CAGAACACGG CCTGCCCAAG CCCGTCATGG ACATGCTGGT GCATCTGTAC 
CTGGAGGGGC CGTACACCAG GGGGGTCTTC AGGAGGTCGG CTGGAGCCAA AGCCTGCCGG 
GAACTCCGGG AGCAACTGGA CAGAGGAACC GAGGATCCAG AGATCACACA TCAATCTGTG 
TTCGTCATCG CTGCTGTCCT GAAGGACTTC CTGCGGAATA TCCCGGGCAG CCTGCTGTGC 
GTGGATCTGT ATGACCGGTG GATGGATGCG ATGGGAACTG AGGGGGCAGA GGAGAAAATA 
CAATCCATCC ACAGGTTGCT CCACCTCCTG CCTGGTGAAA ACCTCCTCCT GCTGCGGCAC 
GTTATCGCCG TGTTGCACTG TATCCAAGGC AACGCTCAAG ACAACCAGAT GAATGCCTTC 
AACTTGTCCG TCTGCATCGC GCCCAGCATG CTTTGGTCCC AGGCACCAAG CACCCCTGAG 
ATGGAGGGGG AGGGCACCAA AAAGGTGTGT GAGCTGGTGC GACTCCTCAT AGAGAACTGC 
TGCGGCGCTC TGGGAGACGA CGTGACGGCG CTGTTCAGAA GCTTCAGCCA GAAGAGCAGC 
AGCAGCGACC ACGGATCAGA TGTGTCGTCC TTCCAGATGA ATGACTCTTC CTACGACAGC 
CTAGAGAACG AGCTGAACGA CGACCCTGAG TCGCCCTACC AGGAGCAGCT GCCATTGCGC 
GACAAGGACA AACCAGACAG TCGCAGCCGT GACTCCGTCA TCACCCTCAG CGACTGTGAC 
CCTGATCCTG ACCCGGAGGC TGAGCTCCTA TTGCAGCTCC CCCAACTGGC CAGGCCGAGG 
AGGTTCAGCC CCGCGGTGCG ACAGCCGCGC CCCCGCCAGG CCAACGGGCA GTCCCAGGGC 
CCCAGAAGGC TGCGGAGGAG TTCGGAACCT GCCTTAACGC TGGAAAGCAT GGCCCCCTCG 
TGCACAGTGA CGGCACTAGC TAACACACAG CGCCTGCCTG TGCGAAAGGC GAGCTACGAT 
GCAGCCATGG AAGGGAAGGG GGAGGAGGAC AGGGTGTTTC TGGAGCAGAG GCTGAGCGGT 
CTTCAGCTGA AGGAGGAGGA AGAGGAGTCG AATAAGAAGG GTGGCGCCAA AGTCCAAAAC 
GGTGGACGGA GGAAGGTGAA GCACACCCCT CCGCCGCCTT TGCGTCTGGA CGCCAGCTGT 
TCCAGCCTGT CGTCACCTGC AACCTCGCCC ACAGGCTCCT CCCTCAGTTC TTTAGACTCC 
GCCTTCTCAC AGTACTCTAC TGACTACACC ACCAATGGTG CCATTCCACT GGCAGAGCCT 
GCACCCCTGT CACCAGGGCG GCCAGAGATG TCACCCAAAG GCTCCCCACC TCAGAGGGAG 
GCCTGGACAC AACCAAGCCA ACCCCCCTCC AATTCTCACG GACTCCATCC CAACACGTGG 
CTCAGTAAGG ACCGCTGCCT GTCACTACGG CAGCCCGAGG AGGACACGCC CGCGGCGAAC 
AGAAAGACTC CAACCAGTAA TGGTTACTCC ACCCTCCAGG AGGCCAAAAG CAGAACCTGC 
CAAAGGAGAT CCAGCAGCCC TCCGTCCTAC CAGCAGGCTC TGCTGCAGCT GCAGCGCAAC 
CGCTCGCCAT TCTATCGAGG CACAGAGAAA CCTCTGACGG TCAAAGAACT GAGGCAGCTC 
CACGACCAGA CTCCCAGCTA CAACCCTAAA CCACAAACAG GAAGTGATGT CACTCCGAGG 
TTGGGAACAA ATGATCATGT GCAGCCCCCA CAGGGAGTTT TCTATGGACA AAGTGGAACC 
ACTTTAGTCC TCCAAAGGCA AAAGTCCCAC CCTCTGACTC AGGACACGAA AGGACAAAAA 
GCAGTAGGGA CCCTTAATCT TCCGCGAAGG GCATCGGAAC CTCCCGTGGT CAACTTAAAC 
CTCACCTCCA GCCTCACGCG TGACAGAGCT CACGCTTCAA AGGGTACAGG AGGATTGAGA 
GTGTCTGAGG TGGAGAGCAG CCACAGCGAG CCACGCTTCT GTCTGTCTCC ATCCGCCACC 
AGGGCAGTGA GGGACTACTT TTCCTCCCAG GGCCAGGAGG ATGCAGACGG CTGCCTGAGG 
AGGAGTCAGG AGGTGGCGCT GGCTATTGTA CAGAGAAAGA GAGAATGGCA GAGCCGGCGG 
TGCAGTGACC CACGAGTGGA TGAGTTTGAG AAACTGTTTT TTGCAGAAGA ATCGTACGTG 
TAA