LOC101062079 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101062079
Entrez Gene ID	101062079
Full Name	cholinergic receptor muscarinic 3
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011602958.2	XP_011601260.1	muscarinic acetylcholine receptor M3
XM_011602957.1	XP_011601259.1	muscarinic acetylcholine receptor M3
XM_011602958.1	XP_011601260.1	muscarinic acetylcholine receptor M3
XM_003963731.2	XP_003963780.1	muscarinic acetylcholine receptor M3
XM_011602957.2	XP_011601259.1	muscarinic acetylcholine receptor M3
XM_029834763.1	XP_029690623.1	muscarinic acetylcholine receptor M3
XM_003963731.3	XP_003963780.1	muscarinic acetylcholine receptor M3

Pathways from BioSystems
Gene Ontology

The following LOC101062079 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101062079 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo02748	XM_011602958.1 Latest version!	Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo02748	XM_003963731.2 Latest version!	Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo02748	XM_011602957.1 Latest version!	Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo64784	XM_003963731.3 Latest version!	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo64784	XM_011602958.2 Latest version!	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo64784	XM_011602957.2 Latest version!	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTo64784	XM_029834763.1 Latest version!	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101062079 ( XM_011602957.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601259.1 Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo02748
Clone ID Related Accession (Same CDS sequence)	XM_011602957.1 , XM_011602958.1 , XM_003963731.2
Accession Version	XM_011602957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070940.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTTCG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601259.1
CDS	254..2200
Translation	MSSNSTDPGLFLTANNSIPAAGAYPQSNPLHQGVTAGAPWLAFHVNPLDDNSSSTGKYLNLTSEAQFGNSTSALDPLGGHPVWQVVLIVLLTGMLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGYWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWFVSLVLWAPPILLWQYFEGKRTVPPDQCIIQFLEQPIITFCTAIAAFYLPVSIMSALYWRIYKETQNRSKELAGLQGSGGRGGIQGRSGNGASQKARFVHQTGSSRSCSSYELTKLTQRKSMCQEFVGRLHCWPGVRSWRPGSIRQGDGDLDQSSSDSWNNNDAAASLDQSGSSDDEDCGSRDMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDAASEEEPLQGVEYNRNSLSTLANNTAATMTPDGANTSEKSCHQRFCSDKSQSTPTVQVASGCSPSQPIAAAADDRTITGSSTKSSSVPMSFKEAAMAKRFASRARTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCKVCIPETLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKQQFQQRSVVFHSRIPREST

Target ORF information:

RefSeq Version	XM_011602957.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602957.1

ORF Insert Sequence:

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTTCG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062079 ( XM_011602958.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601260.1 Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo02748
Clone ID Related Accession (Same CDS sequence)	XM_011602957.1 , XM_011602958.1 , XM_003963731.2
Accession Version	XM_011602958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070940.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTTCG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601260.1
CDS	194..2140
Translation	MSSNSTDPGLFLTANNSIPAAGAYPQSNPLHQGVTAGAPWLAFHVNPLDDNSSSTGKYLNLTSEAQFGNSTSALDPLGGHPVWQVVLIVLLTGMLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGYWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWFVSLVLWAPPILLWQYFEGKRTVPPDQCIIQFLEQPIITFCTAIAAFYLPVSIMSALYWRIYKETQNRSKELAGLQGSGGRGGIQGRSGNGASQKARFVHQTGSSRSCSSYELTKLTQRKSMCQEFVGRLHCWPGVRSWRPGSIRQGDGDLDQSSSDSWNNNDAAASLDQSGSSDDEDCGSRDMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDAASEEEPLQGVEYNRNSLSTLANNTAATMTPDGANTSEKSCHQRFCSDKSQSTPTVQVASGCSPSQPIAAAADDRTITGSSTKSSSVPMSFKEAAMAKRFASRARTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCKVCIPETLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKQQFQQRSVVFHSRIPREST

Target ORF information:

RefSeq Version	XM_011602958.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602958.1

ORF Insert Sequence:

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTTCG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062079 ( XM_003963731.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003963780.1 Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo02748
Clone ID Related Accession (Same CDS sequence)	XM_011602957.1 , XM_011602958.1 , XM_003963731.2
Accession Version	XM_003963731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070940.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003963731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTTCG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003963780.1
CDS	139..2085
Translation	MSSNSTDPGLFLTANNSIPAAGAYPQSNPLHQGVTAGAPWLAFHVNPLDDNSSSTGKYLNLTSEAQFGNSTSALDPLGGHPVWQVVLIVLLTGMLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGYWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWFVSLVLWAPPILLWQYFEGKRTVPPDQCIIQFLEQPIITFCTAIAAFYLPVSIMSALYWRIYKETQNRSKELAGLQGSGGRGGIQGRSGNGASQKARFVHQTGSSRSCSSYELTKLTQRKSMCQEFVGRLHCWPGVRSWRPGSIRQGDGDLDQSSSDSWNNNDAAASLDQSGSSDDEDCGSRDMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDAASEEEPLQGVEYNRNSLSTLANNTAATMTPDGANTSEKSCHQRFCSDKSQSTPTVQVASGCSPSQPIAAAADDRTITGSSTKSSSVPMSFKEAAMAKRFASRARTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCKVCIPETLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKQQFQQRSVVFHSRIPREST

Target ORF information:

RefSeq Version	XM_003963731.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes cholinergic receptor, muscarinic 3 (chrm3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003963731.2

ORF Insert Sequence:

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTTCG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062079 ( XM_011602958.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601260.1 Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo64784
Clone ID Related Accession (Same CDS sequence)	XM_011602958.2 , XM_011602957.2 , XM_029834763.1 , XM_003963731.3
Accession Version	XM_011602958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011602958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601260.1
CDS	243..2189
Translation	MSSNSTDPGLFLTANNSIPAAGAYPQSNPLHQGVTAGAPWLAFHVNPLDDNSSSTGKYLNLTSEAQFGNSTSALDPLGGHPVWQVVLIVLLTGMLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGYWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWFVSLVLWAPPILLWQYFEGKRTVPPDQCIIQFLEQPIITFCTAIAAFYLPVSIMSALYWRIYKETQNRSKELAGLQGSGGRGGIQGRSGNGASQKARFVHQTGSSRSCSSYELTKLTQRKSMCQEFVGRLHCWPGVRSWRPGSIRQGDGDLDQSSSDSWNNNDAAASLDQSGSSDDEDCGSRDMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDAASEEEPLQGVEYNRNSLSTLANNTAATMTPDGANTSEKSCHQRFCSDKSQSTPTVQVASGCSPSQPIAAAADDRTITGSSTKSSSVPMSFKEAAMAKRFASRARTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCKVCIPETLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKQQFQQRSVVFHSRIPREST

Target ORF information:

RefSeq Version	XM_011602958.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602958.2

ORF Insert Sequence:

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062079 ( XM_011602957.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601259.1 Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo64784
Clone ID Related Accession (Same CDS sequence)	XM_011602958.2 , XM_011602957.2 , XM_029834763.1 , XM_003963731.3
Accession Version	XM_011602957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011602957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601259.1
CDS	303..2249
Translation	MSSNSTDPGLFLTANNSIPAAGAYPQSNPLHQGVTAGAPWLAFHVNPLDDNSSSTGKYLNLTSEAQFGNSTSALDPLGGHPVWQVVLIVLLTGMLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGYWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWFVSLVLWAPPILLWQYFEGKRTVPPDQCIIQFLEQPIITFCTAIAAFYLPVSIMSALYWRIYKETQNRSKELAGLQGSGGRGGIQGRSGNGASQKARFVHQTGSSRSCSSYELTKLTQRKSMCQEFVGRLHCWPGVRSWRPGSIRQGDGDLDQSSSDSWNNNDAAASLDQSGSSDDEDCGSRDMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDAASEEEPLQGVEYNRNSLSTLANNTAATMTPDGANTSEKSCHQRFCSDKSQSTPTVQVASGCSPSQPIAAAADDRTITGSSTKSSSVPMSFKEAAMAKRFASRARTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCKVCIPETLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKQQFQQRSVVFHSRIPREST

Target ORF information:

RefSeq Version	XM_011602957.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602957.2

ORF Insert Sequence:

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062079 ( XM_029834763.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029690623.1 Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X2, mRNA.

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CloneID	OTo64784
Clone ID Related Accession (Same CDS sequence)	XM_011602958.2 , XM_011602957.2 , XM_029834763.1 , XM_003963731.3
Accession Version	XM_029834763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029690623.1
CDS	358..2304
Translation	MSSNSTDPGLFLTANNSIPAAGAYPQSNPLHQGVTAGAPWLAFHVNPLDDNSSSTGKYLNLTSEAQFGNSTSALDPLGGHPVWQVVLIVLLTGMLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGYWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWFVSLVLWAPPILLWQYFEGKRTVPPDQCIIQFLEQPIITFCTAIAAFYLPVSIMSALYWRIYKETQNRSKELAGLQGSGGRGGIQGRSGNGASQKARFVHQTGSSRSCSSYELTKLTQRKSMCQEFVGRLHCWPGVRSWRPGSIRQGDGDLDQSSSDSWNNNDAAASLDQSGSSDDEDCGSRDMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDAASEEEPLQGVEYNRNSLSTLANNTAATMTPDGANTSEKSCHQRFCSDKSQSTPTVQVASGCSPSQPIAAAADDRTITGSSTKSSSVPMSFKEAAMAKRFASRARTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCKVCIPETLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKQQFQQRSVVFHSRIPREST

Target ORF information:

RefSeq Version	XM_029834763.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029834763.1

ORF Insert Sequence:

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101062079 ( XM_003963731.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003963780.1 Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X3, mRNA.

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CloneID	OTo64784
Clone ID Related Accession (Same CDS sequence)	XM_011602958.2 , XM_011602957.2 , XM_029834763.1 , XM_003963731.3
Accession Version	XM_003963731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003963731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003963780.1
CDS	188..2134
Translation	MSSNSTDPGLFLTANNSIPAAGAYPQSNPLHQGVTAGAPWLAFHVNPLDDNSSSTGKYLNLTSEAQFGNSTSALDPLGGHPVWQVVLIVLLTGMLSLVTIIGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTAYIVMGYWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAGIMIGLAWFVSLVLWAPPILLWQYFEGKRTVPPDQCIIQFLEQPIITFCTAIAAFYLPVSIMSALYWRIYKETQNRSKELAGLQGSGGRGGIQGRSGNGASQKARFVHQTGSSRSCSSYELTKLTQRKSMCQEFVGRLHCWPGVRSWRPGSIRQGDGDLDQSSSDSWNNNDAAASLDQSGSSDDEDCGSRDMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDAASEEEPLQGVEYNRNSLSTLANNTAATMTPDGANTSEKSCHQRFCSDKSQSTPTVQVASGCSPSQPIAAAADDRTITGSSTKSSSVPMSFKEAAMAKRFASRARTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLINAFCKVCIPETLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKQQFQQRSVVFHSRIPREST

Target ORF information:

RefSeq Version	XM_003963731.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes muscarinic acetylcholine receptor M3 (LOC101062079), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003963731.3

ORF Insert Sequence:

ATGAGCTCAA ACAGCACAGA TCCAGGTCTC TTCCTGACGG CAAACAACTC CATCCCAGCA 
GCTGGAGCCT ACCCACAGTC CAACCCTCTG CACCAGGGAG TAACCGCAGG CGCCCCCTGG 
TTAGCCTTTC ATGTCAATCC CCTGGATGAT AATTCCTCCT CCACCGGCAA ATACTTGAAC 
CTCACCTCAG AAGCCCAATT TGGGAACAGT ACATCAGCTC TTGACCCGTT GGGTGGACAC 
CCCGTGTGGC AGGTTGTGCT GATCGTCTTG TTGACGGGTA TGTTGTCACT GGTCACCATC 
ATTGGCAACA TCCTGGTGGT TGTGTCCTTC AAGGTTAACC GCCAGCTAAA GACAGTGAAT 
AACTATTTCC TGTTGAGTCT GGCAGTGGCA GACCTCATCA TAGGGGTCAT CTCCATGAAC 
CTCTACACAG CTTATATTGT GATGGGCTAC TGGGCCATGG GAAACTGGGC CTGTGACCTG 
TGGCTGGCTA TAGACTATGT GGCCAGTAAT GCTTCAGTTA TGAACCTACT GGTTATCAGC 
TTTGACCGCT ATTTTTCAGT CACCAGGCCT TTGACCTATC GGGCAAAACG GACCACAAAG 
CGAGCTGGGA TCATGATTGG CCTTGCCTGG TTCGTTTCTC TTGTTTTGTG GGCCCCACCA 
ATCCTACTGT GGCAGTATTT TGAGGGTAAA AGGACAGTAC CCCCAGATCA GTGCATCATT 
CAGTTCCTTG AACAGCCCAT CATAACCTTC TGCACAGCCA TTGCAGCCTT CTACCTGCCT 
GTATCAATAA TGAGTGCCCT CTACTGGCGG ATTTACAAGG AGACCCAGAA CCGATCCAAA 
GAGCTGGCAG GGCTGCAGGG CTCTGGAGGC CGAGGTGGGA TACAAGGAAG AAGTGGGAAC 
GGTGCAAGCC AGAAGGCCCG TTTTGTCCAT CAGACAGGGA GTTCTCGTAG TTGCAGTAGT 
TACGAGCTGA CTAAACTGAC GCAGAGAAAG AGCATGTGTC AAGAGTTTGT TGGCCGTTTG 
CACTGCTGGC CTGGTGTACG TTCTTGGAGA CCCGGCAGTA TCCGGCAAGG GGATGGTGAT 
CTAGACCAGA GCAGCAGTGA CAGCTGGAAC AACAATGATG CTGCAGCCTC TTTGGACCAA 
TCTGGATCTT CAGACGATGA AGACTGCGGG AGTCGAGACA TGATTTCCCA GAGTCACGCT 
ATCTTCTCTA TAGTTCTCAG CCTGCCAGGT ATAAAGGCTG CGGTCAACTC TCAGCTCACC 
TCCTGTGAGG ATCTGGATGC AGCCTCAGAG GAGGAGCCAC TTCAAGGGGT TGAGTACAAC 
AGAAACAGCC TGTCAACACT GGCTAACAAC ACTGCTGCCA CCATGACCCC TGATGGAGCC 
AACACTTCTG AAAAGAGTTG TCACCAACGG TTCTGCTCGG ACAAGAGTCA GTCAACTCCT 
ACGGTGCAGG TGGCAAGTGG CTGCTCTCCC AGTCAGCCCA TAGCAGCCGC TGCCGATGAC 
CGCACCATCA CCGGCAGCAG CACAAAGTCC TCCTCCGTCC CAATGTCCTT CAAAGAGGCG 
GCCATGGCGA AGCGATTTGC ATCGCGAGCC CGGACTCAAA TCACGAAGAG GAAGCGCATG 
TCTTTAGTGA AGGAGAAGAA AGCAGCTCAA ACACTCAGCG CTATTCTTCT TGCTTTCATC 
ATCACATGGA CTCCGTACAA CATCATGGTT TTGATAAATG CCTTCTGTAA AGTCTGCATC 
CCAGAGACCC TGTGGGCGGT GGGCTACTGG CTGTGCTACG TCAACAGCAC CGTCAACCCC 
ATGTGTTACG CTCTGTGCAA CAAGACTTTC CGCACAACCT TTAAGATGAT TTTGCTGTGT 
CGCTGGGACC AGAAAAAACG AAGGAAACAG CAGTTTCAAC AGAGGTCGGT GGTCTTCCAC 
AGCAGGATTC CCAGGGAGTC CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC101062079 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム