CCL2 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCL2
Entrez Gene ID	397422
Full Name	chemokine (C-C motif) ligand 2
Synonyms	MCP-1
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214214.1	NP_999379.1	C-C motif chemokine 2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates.
Fishbourne E, Hutet E, Abrams C, Cariolet R, Le Potier MF, Takamatsu HH, Dixon LK
Veterinary research4487(2013 Oct)

Epigallocatechin gallate-mediated protection against tumor necrosis factor-?-induced monocyte chemoattractant protein-1 expression is heme oxygenase-1 dependent.
Zheng Y, Toborek M, Hennig B
Metabolism: clinical and experimental59(10)1528-35(2010 Oct)

Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Moreno S, Alvarez B, Poderoso T, Revilla C, Ezquerra A, Alonso F, Dominguez J
Veterinary research41(5)76(2010 Sep-Oct)

The chemokine monocyte chemoattractant protein-1 contributes to renal dysfunction in swine renovascular hypertension.
Zhu XY, Chade AR, Krier JD, Daghini E, Lavi R, Guglielmotti A, Lerman A, Lerman LO
Journal of hypertension27(10)2063-73(2009 Oct)

Sequential patterns of chemokine- and chemokine receptor-synthesis following vessel wall injury in porcine coronary arteries.
Jabs A, Okamoto E, Vinten-Johansen J, Bauriedel G, Wilcox JN
Atherosclerosis192(1)75-84(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CXCL10 protein was detected in serum from pigs infected with a high virulence African swine fever virus isolate. Levels of CCL2 mRNA were increased in pigs infected with the high virulence isolate compared to a low virulence isolate.
Title: Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates.

The protective properties of flavonoids, such as EGCG, against endothelial inflammation may be regulated in part though induction of HO-1 and subsequent activator protein-1 signaling.
Title: Epigallocatechin gallate-mediated protection against tumor necrosis factor-?-induced monocyte chemoattractant protein-1 expression is heme oxygenase-1 dependent.

Porcine CCL2 binding on Tuk4+SLA-II- but not on Tuk4-SLA-II+ cells was detected by using a CCL2-green fluorescence protein fusion protein.
Title: Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.

MCP-1 contributes to functional and structural impairment in the kidney in renal artery stenosis, mainly in the tubulointerstitial compartment
Title: The chemokine monocyte chemoattractant protein-1 contributes to renal dysfunction in swine renovascular hypertension.

myocardial infarction induced proinflammatory gene and protein expression in peripheral blood mononuclear cells of tissue factor cyclo-oxygenase-2, monocyte chemoattractant protein-1 and CRP
Title: Short-term myocardial ischemia induces cardiac modified C-reactive protein expression and proinflammatory gene (cyclo-oxygenase-2, monocyte chemoattractant protein-1, and tissue factor) upregulation in peripheral blood mononuclear cells.

The following CCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe30420	NM_214214.1 Latest version!	Sus scrofa chemokine (C-C motif) ligand 2 (CCL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCL2 ( NM_214214.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999379.1 Sus scrofa chemokine (C-C motif) ligand 2 (CCL2), mRNA.

Price & Availability↑

CloneID	OSe30420
Clone ID Related Accession (Same CDS sequence)	NM_214214.1
Accession Version	NM_214214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Sus scrofa(pig)
Product	C-C motif chemokine 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X79416.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X79416.1, BX674262.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1487420, SAMEA1876824 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTCT CTGCAGCCCT CCTGTGCCTG CTGCTCACTG CAGCCACCTT CTGCACCCAG 
GTCCTTGCCC AGCCAGATGC AATTAATTCT CCAGTCACCT GCTGCTATAC ACTTACCAGT 
AAGAAGATCT CGATGCAGCG GCTGATGAGC TACAGAAGAG TCACCAGCAG CAAGTGTCCT 
AAAGAAGCAG TGATCTTCAA GACCATCGCG GGCAAGGAGA TCTGTGCAGA ACCCAAGCAG 
AAGTGGGTCC AGGACTCCAT AAGCCACCTG GACAAGAAAA ACCAAACTCC GAAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999379.1
CDS	53..352
Translation	MKVSAALLCLLLTAATFCTQVLAQPDAINSPVTCCYTLTSKKISMQRLMSYRRVTSSKCPKEAVIFKTIAGKEICAEPKQKWVQDSISHLDKKNQTPKP

Target ORF information:

RefSeq Version	NM_214214.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa chemokine (C-C motif) ligand 2 (CCL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214214.1

ORF Insert Sequence:

ATGAAGGTCT CTGCAGCCCT CCTGTGCCTG CTGCTCACTG CAGCCACCTT CTGCACCCAG 
GTCCTTGCCC AGCCAGATGC AATTAATTCT CCAGTCACCT GCTGCTATAC ACTTACCAGT 
AAGAAGATCT CGATGCAGCG GCTGATGAGC TACAGAAGAG TCACCAGCAG CAAGTGTCCT 
AAAGAAGCAG TGATCTTCAA GACCATCGCG GGCAAGGAGA TCTGTGCAGA ACCCAAGCAG 
AAGTGGGTCC AGGACTCCAT AAGCCACCTG GACAAGAAAA ACCAAACTCC GAAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates.
Veterinary research4487(2013 Oct)
Fishbourne E,Hutet E,Abrams C,Cariolet R,Le Potier MF,Takamatsu HH,Dixon LK

Epigallocatechin gallate-mediated protection against tumor necrosis factor-?-induced monocyte chemoattractant protein-1 expression is heme oxygenase-1 dependent.
Metabolism: clinical and experimental59(10)1528-35(2010 Oct)
Zheng Y,Toborek M,Hennig B

Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Veterinary research41(5)76(2010 Sep-Oct)
Moreno S,Alvarez B,Poderoso T,Revilla C,Ezquerra A,Alonso F,Dominguez J

The chemokine monocyte chemoattractant protein-1 contributes to renal dysfunction in swine renovascular hypertension.
Journal of hypertension27(10)2063-73(2009 Oct)
Zhu XY,Chade AR,Krier JD,Daghini E,Lavi R,Guglielmotti A,Lerman A,Lerman LO

Sequential patterns of chemokine- and chemokine receptor-synthesis following vessel wall injury in porcine coronary arteries.
Atherosclerosis192(1)75-84(2007 May)
Jabs A,Okamoto E,Vinten-Johansen J,Bauriedel G,Wilcox JN