ARHGEF28 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF28
Entrez Gene ID	100515652
Full Name	Rho guanine nucleotide exchange factor 28
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021084443.1	XP_020940102.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_021084446.1	XP_020940105.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_013994892.1	XP_013850346.1	rho guanine nucleotide exchange factor 28-like
XM_021084441.1	XP_020940100.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_021084445.1	XP_020940104.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_021084442.1	XP_020940101.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_021084444.1	XP_020940103.1	rho guanine nucleotide exchange factor 28 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARHGEF28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe33105	XM_013994892.1 Latest version!	Sus scrofa rho guanine nucleotide exchange factor 28-like (LOC100515652), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OSe244267	XM_021084444.1 Latest version!	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSe244268	XM_021084445.1 Latest version!	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSe244269	XM_021084446.1 Latest version!	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSe244265	XM_021084443.1 Latest version!	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe244265	XM_021084441.1 Latest version!	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSe244266	XM_021084442.1 Latest version!	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF28 ( XM_013994892.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013850346.1 Sus scrofa rho guanine nucleotide exchange factor 28-like (LOC100515652), mRNA.

Price & Availability↑

CloneID	OSe33105
Clone ID Related Accession (Same CDS sequence)	XM_013994892.1
Accession Version	XM_013994892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	rho guanine nucleotide exchange factor 28-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609627.1) and transcript sequence (AK239952.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 26 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG GTGGTGTGCT CCACCCTGGC CGTTGTTCCG TCCTTCCGTC TCTGTCTCTC 
TCACTGTCTA AATCAGTGTC TCTGCTTCAT CCCTGTAAGC AAAGAGCTTA CAGCTTACCG 
GAGCCACCAA GAGAAAAAAG AATTCAGGAA GAAGAGTGGG ATAAATGCAT CATACCTGCC 
AAATCGGAGT CTGAAAAATA CAAAGTGAGC CGAACATTCA GCTTCCTCAT GAACAGGATG 
ACCAGCCCTC GGAATAAATC TAAGACAAAA GGCAAGGATG CCAAAGACAA AGAGAAACTG 
AGTCGACATC AATTTGTCCC TGGAACATTC TCTGGGGTCC TGCCGTGTTG GGTTTGTGAT 
AAAACTCTCC TGGGGAAAGA GTCGTTCCAG TGTTCCAACT GTAATGCAAA TGTACATAAG 
GGTTGTAAAG ACGCCGCCCC TCCATGTAAC AAGAAATTCC AAGAGAAATA TAACAAGAAC 
AAACCACAGA CCATCCTTGG AAGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013850346.1
CDS	38..2287
Translation	MELNCSEVPLYGQMTVYAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTADNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSDAVTYVDNLACRLARLLVTQADRLTASSHQTLLTPFALTEGALPALDEELVLALTHLELPVGWTVLGNSSLEVSLHRESLLHLAVRWGLTKLSQFLLCLPGGIQALALPNEEGATPIDLALRGGHSKLVEDLTNFQGRRSPGFSRVQLSEGASLQYIHSSETLTLTLNHTADHLVEADIKLFRKYFWDRAFLIKALEEQEARSEEKLDMPSSAAESEEEIKNLVSSRSSPGKESVKCVESPVVQLNEGEGQDGLDLDRFSDVLRKSKRPPLLLAAGRPSDMLNGGDEVYANCMVIDQVGDLDVNYVNIEGITPDTSPESMGSTLGSESSKHILPTETSSGMYPPHGNKDVILHPEAQRSFMSPSSSYASNLNLSFGLHGFEKEQSHLKRRSSSLDALDADSEGEGHSAQSHLCHTPVSQSSSRTGLPSGDELDSFETNTEPDFNISRTESLSLSSSLQLKESLLSGVRSRSYSCSSPKISLGKTRLIRDFTVCNSVEGGVLHPGRCSVLPSLSLSLSKSVSLLHPCKQRAYSLPEPPREKRIQEEEWDKCIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLSRHQFVPGTFSGVLPCWVCDKTLLGKESFQCSNCNANVHKGCKDAAPPCNKKFQEKYNKNKPQTILGSK

Target ORF information:

RefSeq Version	XM_013994892.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa rho guanine nucleotide exchange factor 28-like (LOC100515652), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013994892.1

ORF Insert Sequence:

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG GTGGTGTGCT CCACCCTGGC CGTTGTTCCG TCCTTCCGTC TCTGTCTCTC 
TCACTGTCTA AATCAGTGTC TCTGCTTCAT CCCTGTAAGC AAAGAGCTTA CAGCTTACCG 
GAGCCACCAA GAGAAAAAAG AATTCAGGAA GAAGAGTGGG ATAAATGCAT CATACCTGCC 
AAATCGGAGT CTGAAAAATA CAAAGTGAGC CGAACATTCA GCTTCCTCAT GAACAGGATG 
ACCAGCCCTC GGAATAAATC TAAGACAAAA GGCAAGGATG CCAAAGACAA AGAGAAACTG 
AGTCGACATC AATTTGTCCC TGGAACATTC TCTGGGGTCC TGCCGTGTTG GGTTTGTGAT 
AAAACTCTCC TGGGGAAAGA GTCGTTCCAG TGTTCCAACT GTAATGCAAA TGTACATAAG 
GGTTGTAAAG ACGCCGCCCC TCCATGTAAC AAGAAATTCC AAGAGAAATA TAACAAGAAC 
AAACCACAGA CCATCCTTGG AAGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_021084444.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020940103.1 Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe244267
Clone ID Related Accession (Same CDS sequence)	XM_021084444.1
Accession Version	XM_021084444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCCTGGC CTTCCTTCCC CAAAATGAAG 
ATTCGACGGA GGGCATCCAA ACAAGATCGT TTCTCTGATG TCCTCAGAAA ATCCAAGCGT 
CCTCCCCTGC TCCTTGCTGC TGGCCGTCCT TCTGACATGC TGAATGGCGG TGATGAAGTT 
TATGCTAACT GCATGGTGAT AGATCAGGTT GGTGATCTGG ATGTTAACTA TGTTAATATA 
GAGGGAATCA CTCCGGATAC CAGCCCTGAA TCCATGGGTT CTACTCTGGG GTCTGAGAGC 
AGCAAGCACA TCCTTCCTAC TGAAACCAGC TCTGGCATGT ACCCCCCACA TGGGAACAAG 
GACGTGATAC TCCACCCTGA AGCCCAGCGG TCTTTCATGT CACCATCTAG CTCATATGCT 
TCCAATTTGA ATCTTTCTTT CGGTCTTCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AGAAGAAGCT CCAGTCTTGA TGCCCTGGAT GCCGACAGTG AGGGAGAGGG GCACTCCGCC 
CAGTCGCACC TTTGCCACAC TCCAGTGTCT CAGAGTTCTT CGAGGACTGG CCTTCCTAGT 
GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ATTTTAATAT CTCCAGGACT 
GAATCACTTT CTTTATCAAG TAGCCTGCAG CTGAAGGAAT CACTGCTTTC TGGAGTCCGC 
TCACGTTCTT ATTCCTGCTC ATCACCTAAA ATTTCTTTAG GGAAAACGCG ATTGATACGT 
GATTTTACAG TGTGCAATTC AGTTGAAGGT GGTGTGCTCC ACCCTGGCCG TTGTTCCGTC 
CTTCCGTCTC TGTCTCTCTC ACTGTCTAAA TCAGTGTCTC TGCTTCATCC CTGTAAGCAA 
AGAGCTTACA GCTTACCGGA GCCACCAAGA GAAAAAAGAA TTCAGGAAGA AGAGTGGGAT 
AAATGCATCA TACCTGCCAA ATCGGAGTCT GAAAAATACA AAGTGAGCCG AACATTCAGC 
TTCCTCATGA ACAGGATGAC CAGCCCTCGG AATAAATCTA AGACAAAAGG CAAGGATGCC 
AAAGACAAAG AGAAACTGAG TCGACATCAA TTTGTCCCTG GAACATTCTC TGGGGTCCTG 
CCGTGTTGGG TTTGTGATAA AACTCTCCTG GGGAAAGAGT CGTTCCAGTG TTCCAACTGT 
AATGCAAATG TACATAAGGG TTGTAAAGAC GCCGCCCCTC CATGTAACAA GAAATTCCAA 
GAGAAATATA ACAAGAACAA ACCACAGACC ATCCTTGGAA ATTCTTCATT AAGAGACGGC 
CCACCGCCTG GTCTCTCCCT GCACCCTTCC TCCTCTATGC CTATTGGGTT GCCAACGGGG 
AGAAAGGAAA TGGCCAGCCA GGTCCACCCG CTGTCCAGGA GTGTCCCAGG CACCGCCTTG 
GAAAGCTTCA GGAGGTCGGG GACATCCCTG GAGTCTGAGA GTGATGGCAG CACCTGGAGA 
AGCAGGTCCC ACTCTGATGA GCTGTTCCAG CCCATGGGTT CATCTCCCTC CACCGACTCC 
CTCTTAATGG AAGACGTCGT GGATTCGTCT CTGTGGAGTG ACCTCAGTGG TGACGCCCAG 
GAGTTGGAAG CAGAATCTTG GAGTCTGGTG GTGGACCCCT CATTTTGTAG TAAGCAGGAG 
AAGGATGTCA TCAAAAGACA GGACGTCATT TTTGAGCTGA TGCAAACAGA AATGCATCAC 
ATCCAGACCC TGTTCATCAT GTCTGAGATC TTCAGGAAAG GGATGAAAGA GGAGCTGCAG 
CTGGACCACA GCACTGTGGA TAAAATCTTC CCCTGTTTGG ATGAGTTACT GGAAATCCAC 
AGGCATTTCT TCTATAGTAT GAAGGAGCGA AGGCAAGAAT CCTGTATTGG CAACGACAGG 
AATTTTGTCA TCAACCGCAT CGGAGATATT CTAGTACAAC AGTTTTCAGA AGAAAATGCA 
AATAAAATGA AGAAAATATA TGGGGAATTC TGTAGCCATC ACAAAGAAGC TGTTAGCCTC 
TTTAAAGAAC TCCAGCAGAA TAAAAAGTTC CAGAATTTTA TTAAGCTCCG AAATAGTAAT 
CTTTTGGCTC GACGCCGAGG AATTCCAGAA TGCATTCTGC TGGTCACTCA GCGCATTACG 
AAATACCCTG TCTTGGTGGA AAGGATACTG CAGTACACAA AGGAGAGCAC TGAAGAACAG 
AAAGACTTAT GCAAAGCTCT TTGCTTAATT AAAGACATGA TTGCAGCCGT GGATTTAAAA 
GTCAGCGAGT ATGAGAAAAA ACAAAAATGG CTTGAGATCC TAAGTAAGAT CGAAAACAAA 
ACATACACCA AGCTCAAAAA CGGCCACGTC TTTCGGAAGC AGGCGCTGAT GAGTAAAGAG 
AGGACCCTTC TGTATGAGGG CCTTGTTTAT TGGAAAACTG CAACAGGTCG TTTCAAAGAT 
ATCCTAGCTC TGCTTCTAAC TGATGTGCTG CTCTTTTTAC AAGAAAAAGA CCAGAAATAC 
ATCTTTGCAG CTGTTGATCA GAAGCCGTCC GTTATTTCCC TTCAGAAGCT CATTGCTAGG 
GAAGTTGCGA ATGAGGAGCG AGGAATGTTT CTGATCAGCG CTTCGTCCGC TGGTCCTGAG 
ATGTATGAAA TTCATACCAA CTCCAAGGAG GAACGGAACG CCTGGATGAG ACGGATCCAA 
CAGGCAGTAG AAAGTTGCCC AGAAGAAGAA GGGGGAAAGA CAAGTGAATC AGATGAAGAG 
AGGCGAAGAG CTGAAGTCAG AGTGGCCAAA ATTCAGCAAT GTCAAGAAAT ACTCAGTAAC 
CAAGACCAAC AAATTTGTGC GTATTTGGAG GAGAAGCTGC ATATTTACGC TGAACTTGGA 
GAACTGAGTG GATTTGAGGA TGTCCATCTA GAGCCACACC TCCTCATTAA ACTGGACCCC 
GGAGAGCCTC CCCAGGCGGC CTCGTTACTG GCAGCAGCAC TGAAAGAAGC TGAGAGCCTG 
CAGGTTGCAG TGAAGGTCTC CCAGGTGAGC GATGCGTGTC AGCCCCCCGA GGAAGGCTGT 
GCAGAACCCG CTGTGACGGA TGTGCTCAGT TCTCGGGATG CAGCAGGGTC GCCTACTGCT 
TCACTAGTCA CAGAAGGGAC AGGAGGAAGA GGCTGTTGGG ATGTGGATCC CGGGACCCAG 
GGTGTGGGAA CCGACCAGGC GGTCTCTGAT GCGGGGGAGA AGGTGGAATG TAGAAGTTTC 
CCAGGTTCTT CACAGTCCGA GATTATACAA GCGATACAGA ATTTAACCCG TCTCTTGTAT 
AGCCTGCAGG CCGCCTTGAC CATCCAGGAC AGCCACATTG AGATCCACAA GCTGGTTCTC 
CAGCAGCAGG AGAGCGTGTG TTCTGGCCCT TCTTTCCGAG GCAGCCCCTT GCAGGACCAG 
GAGAAATACC GCAATCTGGA GAAGCGGCGG GAAGAGCTGG CCAGCATCCA CAAGCTGCAG 
CATCAGTTCC TACAGGACCA GCAGCGCTGG CACCGCAGGT GCGACCAGCA GCAGCGGGAG 
CAGGAGGCCA AGGCCAGCCG GCTGCAAGCA CGAGAGCAGG AGTGCCAAGC ACAGGAGGAG 
CTGCTGCTGA GGAATCGGGA GGAGCTGGAC CAGCAGCTCC AGGAGTACCA GCAGAACCTG 
GAGCGGCTAC GGGAGGGCCA ACGCCTGGTG GAGAGGGAAA GAGAGAGGAT GCGGGCCCAG 
CAGAGCCTGC TGTGTGGCTG GAAGCACAGC CGGCAGAGTA GCCTTCCTGC AGCATTTTCT 
CCAGGAAGAA TGGAGCTGAT GGAACTTAAT CGATCTGAGA ATTTCTGTCA TGAAAATTCA 
TTCTTCATCA ATGAAGCTTT AGTGCAGATG TCACTTAACA CTTTCAACAA GCCGACTCCA 
TCAGATGCCA CTTACCCCCC AGATATATCC CATTCTGATT TGGTCAGGAC TTGTGGAAAT 
CAAGTAGCCC TCAAGACGGA CGTTTCCCGG CCCTCAGATG TCAACCATGA ACCGTGGGTA 
GCTGCTGGGC CCTGTCATCA GATACCCCCT CTCCACCAAA GCAGCAAAGA TTCTTGCGAA 
AATGATTTGG ACATCTCTCA GACTGAAAAC TCGCCCCCCC ATGACTCAAA CCCACACGGC 
CCTCAGCTGC AGACGTTTAT AGCAGAAGCA AAGCTAAGTC TACAGAGAGT GACCACACCA 
GATGGGGAAA CTGGAGATGG AGAAGAAAAC ATTGTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020940103.1
CDS	740..4984
Translation	MDSDSDSPFNYSWPSFPKMKIRRRASKQDRFSDVLRKSKRPPLLLAAGRPSDMLNGGDEVYANCMVIDQVGDLDVNYVNIEGITPDTSPESMGSTLGSESSKHILPTETSSGMYPPHGNKDVILHPEAQRSFMSPSSSYASNLNLSFGLHGFEKEQSHLKRRSSSLDALDADSEGEGHSAQSHLCHTPVSQSSSRTGLPSGDELDSFETNTEPDFNISRTESLSLSSSLQLKESLLSGVRSRSYSCSSPKISLGKTRLIRDFTVCNSVEGGVLHPGRCSVLPSLSLSLSKSVSLLHPCKQRAYSLPEPPREKRIQEEEWDKCIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLSRHQFVPGTFSGVLPCWVCDKTLLGKESFQCSNCNANVHKGCKDAAPPCNKKFQEKYNKNKPQTILGNSSLRDGPPPGLSLHPSSSMPIGLPTGRKEMASQVHPLSRSVPGTALESFRRSGTSLESESDGSTWRSRSHSDELFQPMGSSPSTDSLLMEDVVDSSLWSDLSGDAQELEAESWSLVVDPSFCSKQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCIGNDRNFVINRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQKDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILSKIENKTYTKLKNGHVFRKQALMSKERTLLYEGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNAWMRRIQQAVESCPEEEGGKTSESDEERRRAEVRVAKIQQCQEILSNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKLDPGEPPQAASLLAAALKEAESLQVAVKVSQVSDACQPPEEGCAEPAVTDVLSSRDAAGSPTASLVTEGTGGRGCWDVDPGTQGVGTDQAVSDAGEKVECRSFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQQESVCSGPSFRGSPLQDQEKYRNLEKRREELASIHKLQHQFLQDQQRWHRRCDQQQREQEAKASRLQAREQECQAQEELLLRNREELDQQLQEYQQNLERLREGQRLVERERERMRAQQSLLCGWKHSRQSSLPAAFSPGRMELMELNRSENFCHENSFFINEALVQMSLNTFNKPTPSDATYPPDISHSDLVRTCGNQVALKTDVSRPSDVNHEPWVAAGPCHQIPPLHQSSKDSCENDLDISQTENSPPHDSNPHGPQLQTFIAEAKLSLQRVTTPDGETGDGEENIVYL

Target ORF information:

RefSeq Version	XM_021084444.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021084444.1

ORF Insert Sequence:

ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCCTGGC CTTCCTTCCC CAAAATGAAG 
ATTCGACGGA GGGCATCCAA ACAAGATCGT TTCTCTGATG TCCTCAGAAA ATCCAAGCGT 
CCTCCCCTGC TCCTTGCTGC TGGCCGTCCT TCTGACATGC TGAATGGCGG TGATGAAGTT 
TATGCTAACT GCATGGTGAT AGATCAGGTT GGTGATCTGG ATGTTAACTA TGTTAATATA 
GAGGGAATCA CTCCGGATAC CAGCCCTGAA TCCATGGGTT CTACTCTGGG GTCTGAGAGC 
AGCAAGCACA TCCTTCCTAC TGAAACCAGC TCTGGCATGT ACCCCCCACA TGGGAACAAG 
GACGTGATAC TCCACCCTGA AGCCCAGCGG TCTTTCATGT CACCATCTAG CTCATATGCT 
TCCAATTTGA ATCTTTCTTT CGGTCTTCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AGAAGAAGCT CCAGTCTTGA TGCCCTGGAT GCCGACAGTG AGGGAGAGGG GCACTCCGCC 
CAGTCGCACC TTTGCCACAC TCCAGTGTCT CAGAGTTCTT CGAGGACTGG CCTTCCTAGT 
GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ATTTTAATAT CTCCAGGACT 
GAATCACTTT CTTTATCAAG TAGCCTGCAG CTGAAGGAAT CACTGCTTTC TGGAGTCCGC 
TCACGTTCTT ATTCCTGCTC ATCACCTAAA ATTTCTTTAG GGAAAACGCG ATTGATACGT 
GATTTTACAG TGTGCAATTC AGTTGAAGGT GGTGTGCTCC ACCCTGGCCG TTGTTCCGTC 
CTTCCGTCTC TGTCTCTCTC ACTGTCTAAA TCAGTGTCTC TGCTTCATCC CTGTAAGCAA 
AGAGCTTACA GCTTACCGGA GCCACCAAGA GAAAAAAGAA TTCAGGAAGA AGAGTGGGAT 
AAATGCATCA TACCTGCCAA ATCGGAGTCT GAAAAATACA AAGTGAGCCG AACATTCAGC 
TTCCTCATGA ACAGGATGAC CAGCCCTCGG AATAAATCTA AGACAAAAGG CAAGGATGCC 
AAAGACAAAG AGAAACTGAG TCGACATCAA TTTGTCCCTG GAACATTCTC TGGGGTCCTG 
CCGTGTTGGG TTTGTGATAA AACTCTCCTG GGGAAAGAGT CGTTCCAGTG TTCCAACTGT 
AATGCAAATG TACATAAGGG TTGTAAAGAC GCCGCCCCTC CATGTAACAA GAAATTCCAA 
GAGAAATATA ACAAGAACAA ACCACAGACC ATCCTTGGAA ATTCTTCATT AAGAGACGGC 
CCACCGCCTG GTCTCTCCCT GCACCCTTCC TCCTCTATGC CTATTGGGTT GCCAACGGGG 
AGAAAGGAAA TGGCCAGCCA GGTCCACCCG CTGTCCAGGA GTGTCCCAGG CACCGCCTTG 
GAAAGCTTCA GGAGGTCGGG GACATCCCTG GAGTCTGAGA GTGATGGCAG CACCTGGAGA 
AGCAGGTCCC ACTCTGATGA GCTGTTCCAG CCCATGGGTT CATCTCCCTC CACCGACTCC 
CTCTTAATGG AAGACGTCGT GGATTCGTCT CTGTGGAGTG ACCTCAGTGG TGACGCCCAG 
GAGTTGGAAG CAGAATCTTG GAGTCTGGTG GTGGACCCCT CATTTTGTAG TAAGCAGGAG 
AAGGATGTCA TCAAAAGACA GGACGTCATT TTTGAGCTGA TGCAAACAGA AATGCATCAC 
ATCCAGACCC TGTTCATCAT GTCTGAGATC TTCAGGAAAG GGATGAAAGA GGAGCTGCAG 
CTGGACCACA GCACTGTGGA TAAAATCTTC CCCTGTTTGG ATGAGTTACT GGAAATCCAC 
AGGCATTTCT TCTATAGTAT GAAGGAGCGA AGGCAAGAAT CCTGTATTGG CAACGACAGG 
AATTTTGTCA TCAACCGCAT CGGAGATATT CTAGTACAAC AGTTTTCAGA AGAAAATGCA 
AATAAAATGA AGAAAATATA TGGGGAATTC TGTAGCCATC ACAAAGAAGC TGTTAGCCTC 
TTTAAAGAAC TCCAGCAGAA TAAAAAGTTC CAGAATTTTA TTAAGCTCCG AAATAGTAAT 
CTTTTGGCTC GACGCCGAGG AATTCCAGAA TGCATTCTGC TGGTCACTCA GCGCATTACG 
AAATACCCTG TCTTGGTGGA AAGGATACTG CAGTACACAA AGGAGAGCAC TGAAGAACAG 
AAAGACTTAT GCAAAGCTCT TTGCTTAATT AAAGACATGA TTGCAGCCGT GGATTTAAAA 
GTCAGCGAGT ATGAGAAAAA ACAAAAATGG CTTGAGATCC TAAGTAAGAT CGAAAACAAA 
ACATACACCA AGCTCAAAAA CGGCCACGTC TTTCGGAAGC AGGCGCTGAT GAGTAAAGAG 
AGGACCCTTC TGTATGAGGG CCTTGTTTAT TGGAAAACTG CAACAGGTCG TTTCAAAGAT 
ATCCTAGCTC TGCTTCTAAC TGATGTGCTG CTCTTTTTAC AAGAAAAAGA CCAGAAATAC 
ATCTTTGCAG CTGTTGATCA GAAGCCGTCC GTTATTTCCC TTCAGAAGCT CATTGCTAGG 
GAAGTTGCGA ATGAGGAGCG AGGAATGTTT CTGATCAGCG CTTCGTCCGC TGGTCCTGAG 
ATGTATGAAA TTCATACCAA CTCCAAGGAG GAACGGAACG CCTGGATGAG ACGGATCCAA 
CAGGCAGTAG AAAGTTGCCC AGAAGAAGAA GGGGGAAAGA CAAGTGAATC AGATGAAGAG 
AGGCGAAGAG CTGAAGTCAG AGTGGCCAAA ATTCAGCAAT GTCAAGAAAT ACTCAGTAAC 
CAAGACCAAC AAATTTGTGC GTATTTGGAG GAGAAGCTGC ATATTTACGC TGAACTTGGA 
GAACTGAGTG GATTTGAGGA TGTCCATCTA GAGCCACACC TCCTCATTAA ACTGGACCCC 
GGAGAGCCTC CCCAGGCGGC CTCGTTACTG GCAGCAGCAC TGAAAGAAGC TGAGAGCCTG 
CAGGTTGCAG TGAAGGTCTC CCAGGTGAGC GATGCGTGTC AGCCCCCCGA GGAAGGCTGT 
GCAGAACCCG CTGTGACGGA TGTGCTCAGT TCTCGGGATG CAGCAGGGTC GCCTACTGCT 
TCACTAGTCA CAGAAGGGAC AGGAGGAAGA GGCTGTTGGG ATGTGGATCC CGGGACCCAG 
GGTGTGGGAA CCGACCAGGC GGTCTCTGAT GCGGGGGAGA AGGTGGAATG TAGAAGTTTC 
CCAGGTTCTT CACAGTCCGA GATTATACAA GCGATACAGA ATTTAACCCG TCTCTTGTAT 
AGCCTGCAGG CCGCCTTGAC CATCCAGGAC AGCCACATTG AGATCCACAA GCTGGTTCTC 
CAGCAGCAGG AGAGCGTGTG TTCTGGCCCT TCTTTCCGAG GCAGCCCCTT GCAGGACCAG 
GAGAAATACC GCAATCTGGA GAAGCGGCGG GAAGAGCTGG CCAGCATCCA CAAGCTGCAG 
CATCAGTTCC TACAGGACCA GCAGCGCTGG CACCGCAGGT GCGACCAGCA GCAGCGGGAG 
CAGGAGGCCA AGGCCAGCCG GCTGCAAGCA CGAGAGCAGG AGTGCCAAGC ACAGGAGGAG 
CTGCTGCTGA GGAATCGGGA GGAGCTGGAC CAGCAGCTCC AGGAGTACCA GCAGAACCTG 
GAGCGGCTAC GGGAGGGCCA ACGCCTGGTG GAGAGGGAAA GAGAGAGGAT GCGGGCCCAG 
CAGAGCCTGC TGTGTGGCTG GAAGCACAGC CGGCAGAGTA GCCTTCCTGC AGCATTTTCT 
CCAGGAAGAA TGGAGCTGAT GGAACTTAAT CGATCTGAGA ATTTCTGTCA TGAAAATTCA 
TTCTTCATCA ATGAAGCTTT AGTGCAGATG TCACTTAACA CTTTCAACAA GCCGACTCCA 
TCAGATGCCA CTTACCCCCC AGATATATCC CATTCTGATT TGGTCAGGAC TTGTGGAAAT 
CAAGTAGCCC TCAAGACGGA CGTTTCCCGG CCCTCAGATG TCAACCATGA ACCGTGGGTA 
GCTGCTGGGC CCTGTCATCA GATACCCCCT CTCCACCAAA GCAGCAAAGA TTCTTGCGAA 
AATGATTTGG ACATCTCTCA GACTGAAAAC TCGCCCCCCC ATGACTCAAA CCCACACGGC 
CCTCAGCTGC AGACGTTTAT AGCAGAAGCA AAGCTAAGTC TACAGAGAGT GACCACACCA 
GATGGGGAAA CTGGAGATGG AGAAGAAAAC ATTGTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_021084445.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020940104.1 Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

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CloneID	OSe244268
Clone ID Related Accession (Same CDS sequence)	XM_021084445.1
Accession Version	XM_021084445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCCTGGC CTTCCTTCCC CAAAATGAAG 
ATTCGACGGA GGGCATCCAA ACAAGATCGT TTCTCTGATG TCCTCAGAAA ATCCAAGCGT 
CCTCCCCTGC TCCTTGCTGC TGGCCGTCCT TCTGACATGC TGAATGGCGG TGATGAAGTT 
TATGCTAACT GCATGGTGAT AGATCAGGTT GGTGATCTGG ATGTTAACTA TGTTAATATA 
GAGGGAATCA CTCCGGATAC CAGCCCTGAA TCCATGGGTT CTACTCTGGG GTCTGAGAGC 
AGCAAGCACA TCCTTCCTAC TGAAACCAGC TCTGGCATGT ACCCCCCACA TGGGAACAAG 
GACGTGATAC TCCACCCTGA AGCCCAGCGG TCTTTCATGT CACCATCTAG CTCATATGCT 
TCCAATTTGA ATCTTTCTTT CGGTCTTCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AGAAGAAGCT CCAGTCTTGA TGCCCTGGAT GCCGACAGTG AGGGAGAGGG GCACTCCGCC 
CAGTCGCACC TTTGCCACAC TCCAGTGTCT CAGAGTTCTT CGAGGACTGG CCTTCCTAGT 
GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ATTTTAATAT CTCCAGGACT 
GAATCACTTT CTTTATCAAG TAGCCTGCAG CTGAAGGAAT CACTGCTTTC TGGAGTCCGC 
TCACGTTCTT ATTCCTGCTC ATCACCTAAA ATTTCTTTAG GGAAAACGCG ATTGATACGT 
GATTTTACAG TGTGCAATTC AGTTGAAGAG CAAAGAGCTT ACAGCTTACC GGAGCCACCA 
AGAGAAAAAA GAATTCAGGA AGAAGAGTGG GATAAATGCA TCATACCTGC CAAATCGGAG 
TCTGAAAAAT ACAAAGTGAG CCGAACATTC AGCTTCCTCA TGAACAGGAT GACCAGCCCT 
CGGAATAAAT CTAAGACAAA AGGCAAGGAT GCCAAAGACA AAGAGAAACT GAGTCGACAT 
CAATTTGTCC CTGGAACATT CTCTGGGGTC CTGCCGTGTT GGGTTTGTGA TAAAACTCTC 
CTGGGGAAAG AGTCGTTCCA GTGTTCCAAC TGTAATGCAA ATGTACATAA GGGTTGTAAA 
GACGCCGCCC CTCCATGTAA CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTAAGAGAC GGCCCACCGC CTGGTCTCTC CCTGCACCCT 
TCCTCCTCTA TGCCTATTGG GTTGCCAACG GGGAGAAAGG AAATGGCCAG CCAGGTCCAC 
CCGCTGTCCA GGAGTGTCCC AGGCACCGCC TTGGAAAGCT TCAGGAGGTC GGGGACATCC 
CTGGAGTCTG AGAGTGATGG CAGCACCTGG AGAAGCAGGT CCCACTCTGA TGAGCTGTTC 
CAGCCCATGG GTTCATCTCC CTCCACCGAC TCCCTCTTAA TGGAAGACGT CGTGGATTCG 
TCTCTGTGGA GTGACCTCAG TGGTGACGCC CAGGAGTTGG AAGCAGAATC TTGGAGTCTG 
GTGGTGGACC CCTCATTTTG TAGTAAGCAG GAGAAGGATG TCATCAAAAG ACAGGACGTC 
ATTTTTGAGC TGATGCAAAC AGAAATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGGATGAA AGAGGAGCTG CAGCTGGACC ACAGCACTGT GGATAAAATC 
TTCCCCTGTT TGGATGAGTT ACTGGAAATC CACAGGCATT TCTTCTATAG TATGAAGGAG 
CGAAGGCAAG AATCCTGTAT TGGCAACGAC AGGAATTTTG TCATCAACCG CATCGGAGAT 
ATTCTAGTAC AACAGTTTTC AGAAGAAAAT GCAAATAAAA TGAAGAAAAT ATATGGGGAA 
TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTCCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGCTGGTCAC TCAGCGCATT ACGAAATACC CTGTCTTGGT GGAAAGGATA 
CTGCAGTACA CAAAGGAGAG CACTGAAGAA CAGAAAGACT TATGCAAAGC TCTTTGCTTA 
ATTAAAGACA TGATTGCAGC CGTGGATTTA AAAGTCAGCG AGTATGAGAA AAAACAAAAA 
TGGCTTGAGA TCCTAAGTAA GATCGAAAAC AAAACATACA CCAAGCTCAA AAACGGCCAC 
GTCTTTCGGA AGCAGGCGCT GATGAGTAAA GAGAGGACCC TTCTGTATGA GGGCCTTGTT 
TATTGGAAAA CTGCAACAGG TCGTTTCAAA GATATCCTAG CTCTGCTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCG 
TCCGTTATTT CCCTTCAGAA GCTCATTGCT AGGGAAGTTG CGAATGAGGA GCGAGGAATG 
TTTCTGATCA GCGCTTCGTC CGCTGGTCCT GAGATGTATG AAATTCATAC CAACTCCAAG 
GAGGAACGGA ACGCCTGGAT GAGACGGATC CAACAGGCAG TAGAAAGTTG CCCAGAAGAA 
GAAGGGGGAA AGACAAGTGA ATCAGATGAA GAGAGGCGAA GAGCTGAAGT CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATTTA CGCTGAACTT GGAGAACTGA GTGGATTTGA GGATGTCCAT 
CTAGAGCCAC ACCTCCTCAT TAAACTGGAC CCCGGAGAGC CTCCCCAGGC GGCCTCGTTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTGCAGGTTG CAGTGAAGGT CTCCCAGGTG 
AGCGATGCGT GTCAGCCCCC CGAGGAAGGC TGTGCAGAAC CCGCTGTGAC GGATGTGCTC 
AGTTCTCGGG ATGCAGCAGG GTCGCCTACT GCTTCACTAG TCACAGAAGG GACAGGAGGA 
AGAGGCTGTT GGGATGTGGA TCCCGGGACC CAGGGTGTGG GAACCGACCA GGCGGTCTCT 
GATGCGGGGG AGAAGGTGGA ATGTAGAAGT TTCCCAGGTT CTTCACAGTC CGAGATTATA 
CAAGCGATAC AGAATTTAAC CCGTCTCTTG TATAGCCTGC AGGCCGCCTT GACCATCCAG 
GACAGCCACA TTGAGATCCA CAAGCTGGTT CTCCAGCAGC AGGAGAGCGT GTGTTCTGGC 
CCTTCTTTCC GAGGCAGCCC CTTGCAGGAC CAGGAGAAAT ACCGCAATCT GGAGAAGCGG 
CGGGAAGAGC TGGCCAGCAT CCACAAGCTG CAGCATCAGT TCCTACAGGA CCAGCAGCGC 
TGGCACCGCA GGTGCGACCA GCAGCAGCGG GAGCAGGAGG CCAAGGCCAG CCGGCTGCAA 
GCACGAGAGC AGGAGTGCCA AGCACAGGAG GAGCTGCTGC TGAGGAATCG GGAGGAGCTG 
GACCAGCAGC TCCAGGAGTA CCAGCAGAAC CTGGAGCGGC TACGGGAGGG CCAACGCCTG 
GTGGAGAGGG AAAGAGAGAG GATGCGGGCC CAGCAGAGCC TGCTGTGTGG CTGGAAGCAC 
AGCCGGCAGA GTAGCCTTCC TGCAGCATTT TCTCCAGGAA GAATGGAGCT GATGGAACTT 
AATCGATCTG AGAATTTCTG TCATGAAAAT TCATTCTTCA TCAATGAAGC TTTAGTGCAG 
ATGTCACTTA ACACTTTCAA CAAGCCGACT CCATCAGATG CCACTTACCC CCCAGATATA 
TCCCATTCTG ATTTGGTCAG GACTTGTGGA AATCAAGTAG CCCTCAAGAC GGACGTTTCC 
CGGCCCTCAG ATGTCAACCA TGAACCGTGG GTAGCTGCTG GGCCCTGTCA TCAGATACCC 
CCTCTCCACC AAAGCAGCAA AGATTCTTGC GAAAATGATT TGGACATCTC TCAGACTGAA 
AACTCGCCCC CCCATGACTC AAACCCACAC GGCCCTCAGC TGCAGACGTT TATAGCAGAA 
GCAAAGCTAA GTCTACAGAG AGTGACCACA CCAGATGGGG AAACTGGAGA TGGAGAAGAA 
AACATTGTTT ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020940104.1
CDS	739..4896
Translation	MDSDSDSPFNYSWPSFPKMKIRRRASKQDRFSDVLRKSKRPPLLLAAGRPSDMLNGGDEVYANCMVIDQVGDLDVNYVNIEGITPDTSPESMGSTLGSESSKHILPTETSSGMYPPHGNKDVILHPEAQRSFMSPSSSYASNLNLSFGLHGFEKEQSHLKRRSSSLDALDADSEGEGHSAQSHLCHTPVSQSSSRTGLPSGDELDSFETNTEPDFNISRTESLSLSSSLQLKESLLSGVRSRSYSCSSPKISLGKTRLIRDFTVCNSVEEQRAYSLPEPPREKRIQEEEWDKCIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLSRHQFVPGTFSGVLPCWVCDKTLLGKESFQCSNCNANVHKGCKDAAPPCNKKFQEKYNKNKPQTILGNSSLRDGPPPGLSLHPSSSMPIGLPTGRKEMASQVHPLSRSVPGTALESFRRSGTSLESESDGSTWRSRSHSDELFQPMGSSPSTDSLLMEDVVDSSLWSDLSGDAQELEAESWSLVVDPSFCSKQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCIGNDRNFVINRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQKDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILSKIENKTYTKLKNGHVFRKQALMSKERTLLYEGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNAWMRRIQQAVESCPEEEGGKTSESDEERRRAEVRVAKIQQCQEILSNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKLDPGEPPQAASLLAAALKEAESLQVAVKVSQVSDACQPPEEGCAEPAVTDVLSSRDAAGSPTASLVTEGTGGRGCWDVDPGTQGVGTDQAVSDAGEKVECRSFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQQESVCSGPSFRGSPLQDQEKYRNLEKRREELASIHKLQHQFLQDQQRWHRRCDQQQREQEAKASRLQAREQECQAQEELLLRNREELDQQLQEYQQNLERLREGQRLVERERERMRAQQSLLCGWKHSRQSSLPAAFSPGRMELMELNRSENFCHENSFFINEALVQMSLNTFNKPTPSDATYPPDISHSDLVRTCGNQVALKTDVSRPSDVNHEPWVAAGPCHQIPPLHQSSKDSCENDLDISQTENSPPHDSNPHGPQLQTFIAEAKLSLQRVTTPDGETGDGEENIVYL

Target ORF information:

RefSeq Version	XM_021084445.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021084445.1

ORF Insert Sequence:

ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCCTGGC CTTCCTTCCC CAAAATGAAG 
ATTCGACGGA GGGCATCCAA ACAAGATCGT TTCTCTGATG TCCTCAGAAA ATCCAAGCGT 
CCTCCCCTGC TCCTTGCTGC TGGCCGTCCT TCTGACATGC TGAATGGCGG TGATGAAGTT 
TATGCTAACT GCATGGTGAT AGATCAGGTT GGTGATCTGG ATGTTAACTA TGTTAATATA 
GAGGGAATCA CTCCGGATAC CAGCCCTGAA TCCATGGGTT CTACTCTGGG GTCTGAGAGC 
AGCAAGCACA TCCTTCCTAC TGAAACCAGC TCTGGCATGT ACCCCCCACA TGGGAACAAG 
GACGTGATAC TCCACCCTGA AGCCCAGCGG TCTTTCATGT CACCATCTAG CTCATATGCT 
TCCAATTTGA ATCTTTCTTT CGGTCTTCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AGAAGAAGCT CCAGTCTTGA TGCCCTGGAT GCCGACAGTG AGGGAGAGGG GCACTCCGCC 
CAGTCGCACC TTTGCCACAC TCCAGTGTCT CAGAGTTCTT CGAGGACTGG CCTTCCTAGT 
GGGGATGAAC TGGACTCTTT TGAGACCAAC ACTGAACCAG ATTTTAATAT CTCCAGGACT 
GAATCACTTT CTTTATCAAG TAGCCTGCAG CTGAAGGAAT CACTGCTTTC TGGAGTCCGC 
TCACGTTCTT ATTCCTGCTC ATCACCTAAA ATTTCTTTAG GGAAAACGCG ATTGATACGT 
GATTTTACAG TGTGCAATTC AGTTGAAGAG CAAAGAGCTT ACAGCTTACC GGAGCCACCA 
AGAGAAAAAA GAATTCAGGA AGAAGAGTGG GATAAATGCA TCATACCTGC CAAATCGGAG 
TCTGAAAAAT ACAAAGTGAG CCGAACATTC AGCTTCCTCA TGAACAGGAT GACCAGCCCT 
CGGAATAAAT CTAAGACAAA AGGCAAGGAT GCCAAAGACA AAGAGAAACT GAGTCGACAT 
CAATTTGTCC CTGGAACATT CTCTGGGGTC CTGCCGTGTT GGGTTTGTGA TAAAACTCTC 
CTGGGGAAAG AGTCGTTCCA GTGTTCCAAC TGTAATGCAA ATGTACATAA GGGTTGTAAA 
GACGCCGCCC CTCCATGTAA CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTAAGAGAC GGCCCACCGC CTGGTCTCTC CCTGCACCCT 
TCCTCCTCTA TGCCTATTGG GTTGCCAACG GGGAGAAAGG AAATGGCCAG CCAGGTCCAC 
CCGCTGTCCA GGAGTGTCCC AGGCACCGCC TTGGAAAGCT TCAGGAGGTC GGGGACATCC 
CTGGAGTCTG AGAGTGATGG CAGCACCTGG AGAAGCAGGT CCCACTCTGA TGAGCTGTTC 
CAGCCCATGG GTTCATCTCC CTCCACCGAC TCCCTCTTAA TGGAAGACGT CGTGGATTCG 
TCTCTGTGGA GTGACCTCAG TGGTGACGCC CAGGAGTTGG AAGCAGAATC TTGGAGTCTG 
GTGGTGGACC CCTCATTTTG TAGTAAGCAG GAGAAGGATG TCATCAAAAG ACAGGACGTC 
ATTTTTGAGC TGATGCAAAC AGAAATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGGATGAA AGAGGAGCTG CAGCTGGACC ACAGCACTGT GGATAAAATC 
TTCCCCTGTT TGGATGAGTT ACTGGAAATC CACAGGCATT TCTTCTATAG TATGAAGGAG 
CGAAGGCAAG AATCCTGTAT TGGCAACGAC AGGAATTTTG TCATCAACCG CATCGGAGAT 
ATTCTAGTAC AACAGTTTTC AGAAGAAAAT GCAAATAAAA TGAAGAAAAT ATATGGGGAA 
TTCTGTAGCC ATCACAAAGA AGCTGTTAGC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTCCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGCTGGTCAC TCAGCGCATT ACGAAATACC CTGTCTTGGT GGAAAGGATA 
CTGCAGTACA CAAAGGAGAG CACTGAAGAA CAGAAAGACT TATGCAAAGC TCTTTGCTTA 
ATTAAAGACA TGATTGCAGC CGTGGATTTA AAAGTCAGCG AGTATGAGAA AAAACAAAAA 
TGGCTTGAGA TCCTAAGTAA GATCGAAAAC AAAACATACA CCAAGCTCAA AAACGGCCAC 
GTCTTTCGGA AGCAGGCGCT GATGAGTAAA GAGAGGACCC TTCTGTATGA GGGCCTTGTT 
TATTGGAAAA CTGCAACAGG TCGTTTCAAA GATATCCTAG CTCTGCTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCTGTTGA TCAGAAGCCG 
TCCGTTATTT CCCTTCAGAA GCTCATTGCT AGGGAAGTTG CGAATGAGGA GCGAGGAATG 
TTTCTGATCA GCGCTTCGTC CGCTGGTCCT GAGATGTATG AAATTCATAC CAACTCCAAG 
GAGGAACGGA ACGCCTGGAT GAGACGGATC CAACAGGCAG TAGAAAGTTG CCCAGAAGAA 
GAAGGGGGAA AGACAAGTGA ATCAGATGAA GAGAGGCGAA GAGCTGAAGT CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCAGT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATTTA CGCTGAACTT GGAGAACTGA GTGGATTTGA GGATGTCCAT 
CTAGAGCCAC ACCTCCTCAT TAAACTGGAC CCCGGAGAGC CTCCCCAGGC GGCCTCGTTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTGCAGGTTG CAGTGAAGGT CTCCCAGGTG 
AGCGATGCGT GTCAGCCCCC CGAGGAAGGC TGTGCAGAAC CCGCTGTGAC GGATGTGCTC 
AGTTCTCGGG ATGCAGCAGG GTCGCCTACT GCTTCACTAG TCACAGAAGG GACAGGAGGA 
AGAGGCTGTT GGGATGTGGA TCCCGGGACC CAGGGTGTGG GAACCGACCA GGCGGTCTCT 
GATGCGGGGG AGAAGGTGGA ATGTAGAAGT TTCCCAGGTT CTTCACAGTC CGAGATTATA 
CAAGCGATAC AGAATTTAAC CCGTCTCTTG TATAGCCTGC AGGCCGCCTT GACCATCCAG 
GACAGCCACA TTGAGATCCA CAAGCTGGTT CTCCAGCAGC AGGAGAGCGT GTGTTCTGGC 
CCTTCTTTCC GAGGCAGCCC CTTGCAGGAC CAGGAGAAAT ACCGCAATCT GGAGAAGCGG 
CGGGAAGAGC TGGCCAGCAT CCACAAGCTG CAGCATCAGT TCCTACAGGA CCAGCAGCGC 
TGGCACCGCA GGTGCGACCA GCAGCAGCGG GAGCAGGAGG CCAAGGCCAG CCGGCTGCAA 
GCACGAGAGC AGGAGTGCCA AGCACAGGAG GAGCTGCTGC TGAGGAATCG GGAGGAGCTG 
GACCAGCAGC TCCAGGAGTA CCAGCAGAAC CTGGAGCGGC TACGGGAGGG CCAACGCCTG 
GTGGAGAGGG AAAGAGAGAG GATGCGGGCC CAGCAGAGCC TGCTGTGTGG CTGGAAGCAC 
AGCCGGCAGA GTAGCCTTCC TGCAGCATTT TCTCCAGGAA GAATGGAGCT GATGGAACTT 
AATCGATCTG AGAATTTCTG TCATGAAAAT TCATTCTTCA TCAATGAAGC TTTAGTGCAG 
ATGTCACTTA ACACTTTCAA CAAGCCGACT CCATCAGATG CCACTTACCC CCCAGATATA 
TCCCATTCTG ATTTGGTCAG GACTTGTGGA AATCAAGTAG CCCTCAAGAC GGACGTTTCC 
CGGCCCTCAG ATGTCAACCA TGAACCGTGG GTAGCTGCTG GGCCCTGTCA TCAGATACCC 
CCTCTCCACC AAAGCAGCAA AGATTCTTGC GAAAATGATT TGGACATCTC TCAGACTGAA 
AACTCGCCCC CCCATGACTC AAACCCACAC GGCCCTCAGC TGCAGACGTT TATAGCAGAA 
GCAAAGCTAA GTCTACAGAG AGTGACCACA CCAGATGGGG AAACTGGAGA TGGAGAAGAA 
AACATTGTTT ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_021084446.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020940105.1 Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSe244269
Clone ID Related Accession (Same CDS sequence)	XM_021084446.1
Accession Version	XM_021084446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATG GCGGTGATGA AGTTTATGCT AACTGCATGG TGATAGATCA GGTTGGTGAT 
CTGGATGTTA ACTATGTTAA TATAGAGGGA ATCACTCCGG ATACCAGCCC TGAATCCATG 
GGTTCTACTC TGGGGTCTGA GAGCAGCAAG CACATCCTTC CTACTGAAAC CAGCTCTGGC 
ATGTACCCCC CACATGGGAA CAAGGACGTG ATACTCCACC CTGAAGCCCA GCGGTCTTTC 
ATGTCACCAT CTAGCTCATA TGCTTCCAAT TTGAATCTTT CTTTCGGTCT TCATGGATTT 
GAAAAGGAAC AAAGTCATCT AAAGAGAAGA AGCTCCAGTC TTGATGCCCT GGATGCCGAC 
AGTGAGGGAG AGGGGCACTC CGCCCAGTCG CACCTTTGCC ACACTCCAGT GTCTCAGAGT 
TCTTCGAGGA CTGGCCTTCC TAGTGGGGAT GAACTGGACT CTTTTGAGAC CAACACTGAA 
CCAGATTTTA ATATCTCCAG GACTGAATCA CTTTCTTTAT CAAGTAGCCT GCAGCTGAAG 
GAATCACTGC TTTCTGGAGT CCGCTCACGT TCTTATTCCT GCTCATCACC TAAAATTTCT 
TTAGGGAAAA CGCGATTGAT ACGTGATTTT ACAGTGTGCA ATTCAGTTGA AGGTGGTGTG 
CTCCACCCTG GCCGTTGTTC CGTCCTTCCG TCTCTGTCTC TCTCACTGTC TAAATCAGTG 
TCTCTGCTTC ATCCCTGTAA GCAAAGAGCT TACAGCTTAC CGGAGCCACC AAGAGAAAAA 
AGAATTCAGG AAGAAGAGTG GGATAAATGC ATCATACCTG CCAAATCGGA GTCTGAAAAA 
TACAAAGTGA GCCGAACATT CAGCTTCCTC ATGAACAGGA TGACCAGCCC TCGGAATAAA 
TCTAAGACAA AAGGCAAGGA TGCCAAAGAC AAAGAGAAAC TGAGTCGACA TCAATTTGTC 
CCTGGAACAT TCTCTGGGGT CCTGCCGTGT TGGGTTTGTG ATAAAACTCT CCTGGGGAAA 
GAGTCGTTCC AGTGTTCCAA CTGTAATGCA AATGTACATA AGGGTTGTAA AGACGCCGCC 
CCTCCATGTA ACAAGAAATT CCAAGAGAAA TATAACAAGA ACAAACCACA GACCATCCTT 
GGAAATTCTT CATTAAGAGA CGGCCCACCG CCTGGTCTCT CCCTGCACCC TTCCTCCTCT 
ATGCCTATTG GGTTGCCAAC GGGGAGAAAG GAAATGGCCA GCCAGGTCCA CCCGCTGTCC 
AGGAGTGTCC CAGGCACCGC CTTGGAAAGC TTCAGGAGGT CGGGGACATC CCTGGAGTCT 
GAGAGTGATG GCAGCACCTG GAGAAGCAGG TCCCACTCTG ATGAGCTGTT CCAGCCCATG 
GGTTCATCTC CCTCCACCGA CTCCCTCTTA ATGGAAGACG TCGTGGATTC GTCTCTGTGG 
AGTGACCTCA GTGGTGACGC CCAGGAGTTG GAAGCAGAAT CTTGGAGTCT GGTGGTGGAC 
CCCTCATTTT GTAGTAAGCA GGAGAAGGAT GTCATCAAAA GACAGGACGT CATTTTTGAG 
CTGATGCAAA CAGAAATGCA TCACATCCAG ACCCTGTTCA TCATGTCTGA GATCTTCAGG 
AAAGGGATGA AAGAGGAGCT GCAGCTGGAC CACAGCACTG TGGATAAAAT CTTCCCCTGT 
TTGGATGAGT TACTGGAAAT CCACAGGCAT TTCTTCTATA GTATGAAGGA GCGAAGGCAA 
GAATCCTGTA TTGGCAACGA CAGGAATTTT GTCATCAACC GCATCGGAGA TATTCTAGTA 
CAACAGTTTT CAGAAGAAAA TGCAAATAAA ATGAAGAAAA TATATGGGGA ATTCTGTAGC 
CATCACAAAG AAGCTGTTAG CCTCTTTAAA GAACTCCAGC AGAATAAAAA GTTCCAGAAT 
TTTATTAAGC TCCGAAATAG TAATCTTTTG GCTCGACGCC GAGGAATTCC AGAATGCATT 
CTGCTGGTCA CTCAGCGCAT TACGAAATAC CCTGTCTTGG TGGAAAGGAT ACTGCAGTAC 
ACAAAGGAGA GCACTGAAGA ACAGAAAGAC TTATGCAAAG CTCTTTGCTT AATTAAAGAC 
ATGATTGCAG CCGTGGATTT AAAAGTCAGC GAGTATGAGA AAAAACAAAA ATGGCTTGAG 
ATCCTAAGTA AGATCGAAAA CAAAACATAC ACCAAGCTCA AAAACGGCCA CGTCTTTCGG 
AAGCAGGCGC TGATGAGTAA AGAGAGGACC CTTCTGTATG AGGGCCTTGT TTATTGGAAA 
ACTGCAACAG GTCGTTTCAA AGATATCCTA GCTCTGCTTC TAACTGATGT GCTGCTCTTT 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ATCAGAAGCC GTCCGTTATT 
TCCCTTCAGA AGCTCATTGC TAGGGAAGTT GCGAATGAGG AGCGAGGAAT GTTTCTGATC 
AGCGCTTCGT CCGCTGGTCC TGAGATGTAT GAAATTCATA CCAACTCCAA GGAGGAACGG 
AACGCCTGGA TGAGACGGAT CCAACAGGCA GTAGAAAGTT GCCCAGAAGA AGAAGGGGGA 
AAGACAAGTG AATCAGATGA AGAGAGGCGA AGAGCTGAAG TCAGAGTGGC CAAAATTCAG 
CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAAATTT GTGCGTATTT GGAGGAGAAG 
CTGCATATTT ACGCTGAACT TGGAGAACTG AGTGGATTTG AGGATGTCCA TCTAGAGCCA 
CACCTCCTCA TTAAACTGGA CCCCGGAGAG CCTCCCCAGG CGGCCTCGTT ACTGGCAGCA 
GCACTGAAAG AAGCTGAGAG CCTGCAGGTT GCAGTGAAGG TCTCCCAGGT GAGCGATGCG 
TGTCAGCCCC CCGAGGAAGG CTGTGCAGAA CCCGCTGTGA CGGATGTGCT CAGTTCTCGG 
GATGCAGCAG GGTCGCCTAC TGCTTCACTA GTCACAGAAG GGACAGGAGG AAGAGGCTGT 
TGGGATGTGG ATCCCGGGAC CCAGGGTGTG GGAACCGACC AGGCGGTCTC TGATGCGGGG 
GAGAAGGTGG AATGTAGAAG TTTCCCAGGT TCTTCACAGT CCGAGATTAT ACAAGCGATA 
CAGAATTTAA CCCGTCTCTT GTATAGCCTG CAGGCCGCCT TGACCATCCA GGACAGCCAC 
ATTGAGATCC ACAAGCTGGT TCTCCAGCAG CAGGAGAGCG TGTGTTCTGG CCCTTCTTTC 
CGAGGCAGCC CCTTGCAGGA CCAGGAGAAA TACCGCAATC TGGAGAAGCG GCGGGAAGAG 
CTGGCCAGCA TCCACAAGCT GCAGCATCAG TTCCTACAGG ACCAGCAGCG CTGGCACCGC 
AGGTGCGACC AGCAGCAGCG GGAGCAGGAG GCCAAGGCCA GCCGGCTGCA AGCACGAGAG 
CAGGAGTGCC AAGCACAGGA GGAGCTGCTG CTGAGGAATC GGGAGGAGCT GGACCAGCAG 
CTCCAGGAGT ACCAGCAGAA CCTGGAGCGG CTACGGGAGG GCCAACGCCT GGTGGAGAGG 
GAAAGAGAGA GGATGCGGGC CCAGCAGAGC CTGCTGTGTG GCTGGAAGCA CAGCCGGCAG 
AGTAGCCTTC CTGCAGCATT TTCTCCAGGA AGAATGGAGC TGATGGAACT TAATCGATCT 
GAGAATTTCT GTCATGAAAA TTCATTCTTC ATCAATGAAG CTTTAGTGCA GATGTCACTT 
AACACTTTCA ACAAGCCGAC TCCATCAGAT GCCACTTACC CCCCAGATAT ATCCCATTCT 
GATTTGGTCA GGACTTGTGG AAATCAAGTA GCCCTCAAGA CGGACGTTTC CCGGCCCTCA 
GATGTCAACC ATGAACCGTG GGTAGCTGCT GGGCCCTGTC ATCAGATACC CCCTCTCCAC 
CAAAGCAGCA AAGATTCTTG CGAAAATGAT TTGGACATCT CTCAGACTGA AAACTCGCCC 
CCCCATGACT CAAACCCACA CGGCCCTCAG CTGCAGACGT TTATAGCAGA AGCAAAGCTA 
AGTCTACAGA GAGTGACCAC ACCAGATGGG GAAACTGGAG ATGGAGAAGA AAACATTGTT 
TACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020940105.1
CDS	4961..9049
Translation	MLNGGDEVYANCMVIDQVGDLDVNYVNIEGITPDTSPESMGSTLGSESSKHILPTETSSGMYPPHGNKDVILHPEAQRSFMSPSSSYASNLNLSFGLHGFEKEQSHLKRRSSSLDALDADSEGEGHSAQSHLCHTPVSQSSSRTGLPSGDELDSFETNTEPDFNISRTESLSLSSSLQLKESLLSGVRSRSYSCSSPKISLGKTRLIRDFTVCNSVEGGVLHPGRCSVLPSLSLSLSKSVSLLHPCKQRAYSLPEPPREKRIQEEEWDKCIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLSRHQFVPGTFSGVLPCWVCDKTLLGKESFQCSNCNANVHKGCKDAAPPCNKKFQEKYNKNKPQTILGNSSLRDGPPPGLSLHPSSSMPIGLPTGRKEMASQVHPLSRSVPGTALESFRRSGTSLESESDGSTWRSRSHSDELFQPMGSSPSTDSLLMEDVVDSSLWSDLSGDAQELEAESWSLVVDPSFCSKQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCIGNDRNFVINRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQKDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILSKIENKTYTKLKNGHVFRKQALMSKERTLLYEGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNAWMRRIQQAVESCPEEEGGKTSESDEERRRAEVRVAKIQQCQEILSNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKLDPGEPPQAASLLAAALKEAESLQVAVKVSQVSDACQPPEEGCAEPAVTDVLSSRDAAGSPTASLVTEGTGGRGCWDVDPGTQGVGTDQAVSDAGEKVECRSFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQQESVCSGPSFRGSPLQDQEKYRNLEKRREELASIHKLQHQFLQDQQRWHRRCDQQQREQEAKASRLQAREQECQAQEELLLRNREELDQQLQEYQQNLERLREGQRLVERERERMRAQQSLLCGWKHSRQSSLPAAFSPGRMELMELNRSENFCHENSFFINEALVQMSLNTFNKPTPSDATYPPDISHSDLVRTCGNQVALKTDVSRPSDVNHEPWVAAGPCHQIPPLHQSSKDSCENDLDISQTENSPPHDSNPHGPQLQTFIAEAKLSLQRVTTPDGETGDGEENIVYL

Target ORF information:

RefSeq Version	XM_021084446.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021084446.1

ORF Insert Sequence:

ATGCTGAATG GCGGTGATGA AGTTTATGCT AACTGCATGG TGATAGATCA GGTTGGTGAT 
CTGGATGTTA ACTATGTTAA TATAGAGGGA ATCACTCCGG ATACCAGCCC TGAATCCATG 
GGTTCTACTC TGGGGTCTGA GAGCAGCAAG CACATCCTTC CTACTGAAAC CAGCTCTGGC 
ATGTACCCCC CACATGGGAA CAAGGACGTG ATACTCCACC CTGAAGCCCA GCGGTCTTTC 
ATGTCACCAT CTAGCTCATA TGCTTCCAAT TTGAATCTTT CTTTCGGTCT TCATGGATTT 
GAAAAGGAAC AAAGTCATCT AAAGAGAAGA AGCTCCAGTC TTGATGCCCT GGATGCCGAC 
AGTGAGGGAG AGGGGCACTC CGCCCAGTCG CACCTTTGCC ACACTCCAGT GTCTCAGAGT 
TCTTCGAGGA CTGGCCTTCC TAGTGGGGAT GAACTGGACT CTTTTGAGAC CAACACTGAA 
CCAGATTTTA ATATCTCCAG GACTGAATCA CTTTCTTTAT CAAGTAGCCT GCAGCTGAAG 
GAATCACTGC TTTCTGGAGT CCGCTCACGT TCTTATTCCT GCTCATCACC TAAAATTTCT 
TTAGGGAAAA CGCGATTGAT ACGTGATTTT ACAGTGTGCA ATTCAGTTGA AGGTGGTGTG 
CTCCACCCTG GCCGTTGTTC CGTCCTTCCG TCTCTGTCTC TCTCACTGTC TAAATCAGTG 
TCTCTGCTTC ATCCCTGTAA GCAAAGAGCT TACAGCTTAC CGGAGCCACC AAGAGAAAAA 
AGAATTCAGG AAGAAGAGTG GGATAAATGC ATCATACCTG CCAAATCGGA GTCTGAAAAA 
TACAAAGTGA GCCGAACATT CAGCTTCCTC ATGAACAGGA TGACCAGCCC TCGGAATAAA 
TCTAAGACAA AAGGCAAGGA TGCCAAAGAC AAAGAGAAAC TGAGTCGACA TCAATTTGTC 
CCTGGAACAT TCTCTGGGGT CCTGCCGTGT TGGGTTTGTG ATAAAACTCT CCTGGGGAAA 
GAGTCGTTCC AGTGTTCCAA CTGTAATGCA AATGTACATA AGGGTTGTAA AGACGCCGCC 
CCTCCATGTA ACAAGAAATT CCAAGAGAAA TATAACAAGA ACAAACCACA GACCATCCTT 
GGAAATTCTT CATTAAGAGA CGGCCCACCG CCTGGTCTCT CCCTGCACCC TTCCTCCTCT 
ATGCCTATTG GGTTGCCAAC GGGGAGAAAG GAAATGGCCA GCCAGGTCCA CCCGCTGTCC 
AGGAGTGTCC CAGGCACCGC CTTGGAAAGC TTCAGGAGGT CGGGGACATC CCTGGAGTCT 
GAGAGTGATG GCAGCACCTG GAGAAGCAGG TCCCACTCTG ATGAGCTGTT CCAGCCCATG 
GGTTCATCTC CCTCCACCGA CTCCCTCTTA ATGGAAGACG TCGTGGATTC GTCTCTGTGG 
AGTGACCTCA GTGGTGACGC CCAGGAGTTG GAAGCAGAAT CTTGGAGTCT GGTGGTGGAC 
CCCTCATTTT GTAGTAAGCA GGAGAAGGAT GTCATCAAAA GACAGGACGT CATTTTTGAG 
CTGATGCAAA CAGAAATGCA TCACATCCAG ACCCTGTTCA TCATGTCTGA GATCTTCAGG 
AAAGGGATGA AAGAGGAGCT GCAGCTGGAC CACAGCACTG TGGATAAAAT CTTCCCCTGT 
TTGGATGAGT TACTGGAAAT CCACAGGCAT TTCTTCTATA GTATGAAGGA GCGAAGGCAA 
GAATCCTGTA TTGGCAACGA CAGGAATTTT GTCATCAACC GCATCGGAGA TATTCTAGTA 
CAACAGTTTT CAGAAGAAAA TGCAAATAAA ATGAAGAAAA TATATGGGGA ATTCTGTAGC 
CATCACAAAG AAGCTGTTAG CCTCTTTAAA GAACTCCAGC AGAATAAAAA GTTCCAGAAT 
TTTATTAAGC TCCGAAATAG TAATCTTTTG GCTCGACGCC GAGGAATTCC AGAATGCATT 
CTGCTGGTCA CTCAGCGCAT TACGAAATAC CCTGTCTTGG TGGAAAGGAT ACTGCAGTAC 
ACAAAGGAGA GCACTGAAGA ACAGAAAGAC TTATGCAAAG CTCTTTGCTT AATTAAAGAC 
ATGATTGCAG CCGTGGATTT AAAAGTCAGC GAGTATGAGA AAAAACAAAA ATGGCTTGAG 
ATCCTAAGTA AGATCGAAAA CAAAACATAC ACCAAGCTCA AAAACGGCCA CGTCTTTCGG 
AAGCAGGCGC TGATGAGTAA AGAGAGGACC CTTCTGTATG AGGGCCTTGT TTATTGGAAA 
ACTGCAACAG GTCGTTTCAA AGATATCCTA GCTCTGCTTC TAACTGATGT GCTGCTCTTT 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ATCAGAAGCC GTCCGTTATT 
TCCCTTCAGA AGCTCATTGC TAGGGAAGTT GCGAATGAGG AGCGAGGAAT GTTTCTGATC 
AGCGCTTCGT CCGCTGGTCC TGAGATGTAT GAAATTCATA CCAACTCCAA GGAGGAACGG 
AACGCCTGGA TGAGACGGAT CCAACAGGCA GTAGAAAGTT GCCCAGAAGA AGAAGGGGGA 
AAGACAAGTG AATCAGATGA AGAGAGGCGA AGAGCTGAAG TCAGAGTGGC CAAAATTCAG 
CAATGTCAAG AAATACTCAG TAACCAAGAC CAACAAATTT GTGCGTATTT GGAGGAGAAG 
CTGCATATTT ACGCTGAACT TGGAGAACTG AGTGGATTTG AGGATGTCCA TCTAGAGCCA 
CACCTCCTCA TTAAACTGGA CCCCGGAGAG CCTCCCCAGG CGGCCTCGTT ACTGGCAGCA 
GCACTGAAAG AAGCTGAGAG CCTGCAGGTT GCAGTGAAGG TCTCCCAGGT GAGCGATGCG 
TGTCAGCCCC CCGAGGAAGG CTGTGCAGAA CCCGCTGTGA CGGATGTGCT CAGTTCTCGG 
GATGCAGCAG GGTCGCCTAC TGCTTCACTA GTCACAGAAG GGACAGGAGG AAGAGGCTGT 
TGGGATGTGG ATCCCGGGAC CCAGGGTGTG GGAACCGACC AGGCGGTCTC TGATGCGGGG 
GAGAAGGTGG AATGTAGAAG TTTCCCAGGT TCTTCACAGT CCGAGATTAT ACAAGCGATA 
CAGAATTTAA CCCGTCTCTT GTATAGCCTG CAGGCCGCCT TGACCATCCA GGACAGCCAC 
ATTGAGATCC ACAAGCTGGT TCTCCAGCAG CAGGAGAGCG TGTGTTCTGG CCCTTCTTTC 
CGAGGCAGCC CCTTGCAGGA CCAGGAGAAA TACCGCAATC TGGAGAAGCG GCGGGAAGAG 
CTGGCCAGCA TCCACAAGCT GCAGCATCAG TTCCTACAGG ACCAGCAGCG CTGGCACCGC 
AGGTGCGACC AGCAGCAGCG GGAGCAGGAG GCCAAGGCCA GCCGGCTGCA AGCACGAGAG 
CAGGAGTGCC AAGCACAGGA GGAGCTGCTG CTGAGGAATC GGGAGGAGCT GGACCAGCAG 
CTCCAGGAGT ACCAGCAGAA CCTGGAGCGG CTACGGGAGG GCCAACGCCT GGTGGAGAGG 
GAAAGAGAGA GGATGCGGGC CCAGCAGAGC CTGCTGTGTG GCTGGAAGCA CAGCCGGCAG 
AGTAGCCTTC CTGCAGCATT TTCTCCAGGA AGAATGGAGC TGATGGAACT TAATCGATCT 
GAGAATTTCT GTCATGAAAA TTCATTCTTC ATCAATGAAG CTTTAGTGCA GATGTCACTT 
AACACTTTCA ACAAGCCGAC TCCATCAGAT GCCACTTACC CCCCAGATAT ATCCCATTCT 
GATTTGGTCA GGACTTGTGG AAATCAAGTA GCCCTCAAGA CGGACGTTTC CCGGCCCTCA 
GATGTCAACC ATGAACCGTG GGTAGCTGCT GGGCCCTGTC ATCAGATACC CCCTCTCCAC 
CAAAGCAGCA AAGATTCTTG CGAAAATGAT TTGGACATCT CTCAGACTGA AAACTCGCCC 
CCCCATGACT CAAACCCACA CGGCCCTCAG CTGCAGACGT TTATAGCAGA AGCAAAGCTA 
AGTCTACAGA GAGTGACCAC ACCAGATGGG GAAACTGGAG ATGGAGAAGA AAACATTGTT 
TACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_021084443.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020940102.1 Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe244265
Clone ID Related Accession (Same CDS sequence)	XM_021084443.1 , XM_021084441.1
Accession Version	XM_021084443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG GTGGTGTGCT CCACCCTGGC CGTTGTTCCG TCCTTCCGTC TCTGTCTCTC 
TCACTGTCTA AATCAGTGTC TCTGCTTCAT CCCTGTAAGC AAAGAGCTTA CAGCTTACCG 
GAGCCACCAA GAGAAAAAAG AATTCAGGAA GAAGAGTGGG ATAAATGCAT CATACCTGCC 
AAATCGGAGT CTGAAAAATA CAAAGTGAGC CGAACATTCA GCTTCCTCAT GAACAGGATG 
ACCAGCCCTC GGAATAAATC TAAGACAAAA GGCAAGGATG CCAAAGACAA AGAGAAACTG 
AGTCGACATC AATTTGTCCC TGGAACATTC TCTGGGGTCC TGCCGTGTTG GGTTTGTGAT 
AAAACTCTCC TGGGGAAAGA GTCGTTCCAG TGTTCCAACT GTAATGCAAA TGTACATAAG 
GGTTGTAAAG ACGCCGCCCC TCCATGTAAC AAGAAATTCC AAGAGAAATA TAACAAGAAC 
AAACCACAGA CCATCCTTGG AAATTCTTCA TTAAGAGACG GCCCACCGCC TGGTCTCTCC 
CTGCACCCTT CCTCCTCTAT GCCTATTGGG TTGCCAACGG GGAGAAAGGA AATGGCCAGC 
CAGGTCCACC CGCTGTCCAG GAGTGTCCCA GGCACCGCCT TGGAAAGCTT CAGGAGGTCG 
GGGACATCCC TGGAGTCTGA GAGTGATGGC AGCACCTGGA GAAGCAGGTC CCACTCTGAT 
GAGCTGTTCC AGCCCATGGG TTCATCTCCC TCCACCGACT CCCTCTTAAT GGAAGACGTC 
GTGGATTCGT CTCTGTGGAG TGACCTCAGT GGTGACGCCC AGGAGTTGGA AGCAGAATCT 
TGGAGTCTGG TGGTGGACCC CTCATTTTGT AGTAAGCAGG AGAAGGATGT CATCAAAAGA 
CAGGACGTCA TTTTTGAGCT GATGCAAACA GAAATGCATC ACATCCAGAC CCTGTTCATC 
ATGTCTGAGA TCTTCAGGAA AGGGATGAAA GAGGAGCTGC AGCTGGACCA CAGCACTGTG 
GATAAAATCT TCCCCTGTTT GGATGAGTTA CTGGAAATCC ACAGGCATTT CTTCTATAGT 
ATGAAGGAGC GAAGGCAAGA ATCCTGTATT GGCAACGACA GGAATTTTGT CATCAACCGC 
ATCGGAGATA TTCTAGTACA ACAGTTTTCA GAAGAAAATG CAAATAAAAT GAAGAAAATA 
TATGGGGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG 
AATAAAAAGT TCCAGAATTT TATTAAGCTC CGAAATAGTA ATCTTTTGGC TCGACGCCGA 
GGAATTCCAG AATGCATTCT GCTGGTCACT CAGCGCATTA CGAAATACCC TGTCTTGGTG 
GAAAGGATAC TGCAGTACAC AAAGGAGAGC ACTGAAGAAC AGAAAGACTT ATGCAAAGCT 
CTTTGCTTAA TTAAAGACAT GATTGCAGCC GTGGATTTAA AAGTCAGCGA GTATGAGAAA 
AAACAAAAAT GGCTTGAGAT CCTAAGTAAG ATCGAAAACA AAACATACAC CAAGCTCAAA 
AACGGCCACG TCTTTCGGAA GCAGGCGCTG ATGAGTAAAG AGAGGACCCT TCTGTATGAG 
GGCCTTGTTT ATTGGAAAAC TGCAACAGGT CGTTTCAAAG ATATCCTAGC TCTGCTTCTA 
ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT 
CAGAAGCCGT CCGTTATTTC CCTTCAGAAG CTCATTGCTA GGGAAGTTGC GAATGAGGAG 
CGAGGAATGT TTCTGATCAG CGCTTCGTCC GCTGGTCCTG AGATGTATGA AATTCATACC 
AACTCCAAGG AGGAACGGAA CGCCTGGATG AGACGGATCC AACAGGCAGT AGAAAGTTGC 
CCAGAAGAAG AAGGGGGAAA GACAAGTGAA TCAGATGAAG AGAGGCGAAG AGCTGAAGTC 
AGAGTGGCCA AAATTCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAAATTTGT 
GCGTATTTGG AGGAGAAGCT GCATATTTAC GCTGAACTTG GAGAACTGAG TGGATTTGAG 
GATGTCCATC TAGAGCCACA CCTCCTCATT AAACTGGACC CCGGAGAGCC TCCCCAGGCG 
GCCTCGTTAC TGGCAGCAGC ACTGAAAGAA GCTGAGAGCC TGCAGGTTGC AGTGAAGGTC 
TCCCAGGTGA GCGATGCGTG TCAGCCCCCC GAGGAAGGCT GTGCAGAACC CGCTGTGACG 
GATGTGCTCA GTTCTCGGGA TGCAGCAGGG TCGCCTACTG CTTCACTAGT CACAGAAGGG 
ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCCGGGACCC AGGGTGTGGG AACCGACCAG 
GCGGTCTCTG ATGCGGGGGA GAAGGTGGAA TGTAGAAGTT TCCCAGGTTC TTCACAGTCC 
GAGATTATAC AAGCGATACA GAATTTAACC CGTCTCTTGT ATAGCCTGCA GGCCGCCTTG 
ACCATCCAGG ACAGCCACAT TGAGATCCAC AAGCTGGTTC TCCAGCAGCA GGAGAGCGTG 
TGTTCTGGCC CTTCTTTCCG AGGCAGCCCC TTGCAGGACC AGGAGAAATA CCGCAATCTG 
GAGAAGCGGC GGGAAGAGCT GGCCAGCATC CACAAGCTGC AGCATCAGTT CCTACAGGAC 
CAGCAGCGCT GGCACCGCAG GTGCGACCAG CAGCAGCGGG AGCAGGAGGC CAAGGCCAGC 
CGGCTGCAAG CACGAGAGCA GGAGTGCCAA GCACAGGAGG AGCTGCTGCT GAGGAATCGG 
GAGGAGCTGG ACCAGCAGCT CCAGGAGTAC CAGCAGAACC TGGAGCGGCT ACGGGAGGGC 
CAACGCCTGG TGGAGAGGGA AAGAGAGAGG ATGCGGGCCC AGCAGAGCCT GCTGTGTGGC 
TGGAAGCACA GCCGGCAGAG TAGCCTTCCT GCAGCATTTT CTCCAGGAAG AATGGAGCTG 
ATGGAACTTA ATCGATCTGA GAATTTCTGT CATGAAAATT CATTCTTCAT CAATGAAGCT 
TTAGTGCAGA TGTCACTTAA CACTTTCAAC AAGCCGACTC CATCAGATGC CACTTACCCC 
CCAGATATAT CCCATTCTGA TTTGGTCAGG ACTTGTGGAA ATCAAGTAGC CCTCAAGACG 
GACGTTTCCC GGCCCTCAGA TGTCAACCAT GAACCGTGGG TAGCTGCTGG GCCCTGTCAT 
CAGATACCCC CTCTCCACCA AAGCAGCAAA GATTCTTGCG AAAATGATTT GGACATCTCT 
CAGACTGAAA ACTCGCCCCC CCATGACTCA AACCCACACG GCCCTCAGCT GCAGACGTTT 
ATAGCAGAAG CAAAGCTAAG TCTACAGAGA GTGACCACAC CAGATGGGGA AACTGGAGAT 
GGAGAAGAAA ACATTGTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020940102.1
CDS	152..5338
Translation	MELNCSEVPLYGQMTVYAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTADNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSDAVTYVDNLACRLARLLVTQADRLTASSHQTLLTPFALTEGALPALDEELVLALTHLELPVGWTVLGNSSLEVSLHRESLLHLAVRWGLTKLSQFLLCLPGGIQALALPNEEGATPIDLALRGGHSKLVEDLTNFQGRRSPGFSRVQLSEGASLQYIHSSETLTLTLNHTADHLVEADIKLFRKYFWDRAFLIKALEEQEARSEEKLDMPSSAAESEEEIKNLVSSRSSPGKESVKCVESPVVQLNEGEGQDGLDLDRFSDVLRKSKRPPLLLAAGRPSDMLNGGDEVYANCMVIDQVGDLDVNYVNIEGITPDTSPESMGSTLGSESSKHILPTETSSGMYPPHGNKDVILHPEAQRSFMSPSSSYASNLNLSFGLHGFEKEQSHLKRRSSSLDALDADSEGEGHSAQSHLCHTPVSQSSSRTGLPSGDELDSFETNTEPDFNISRTESLSLSSSLQLKESLLSGVRSRSYSCSSPKISLGKTRLIRDFTVCNSVEGGVLHPGRCSVLPSLSLSLSKSVSLLHPCKQRAYSLPEPPREKRIQEEEWDKCIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLSRHQFVPGTFSGVLPCWVCDKTLLGKESFQCSNCNANVHKGCKDAAPPCNKKFQEKYNKNKPQTILGNSSLRDGPPPGLSLHPSSSMPIGLPTGRKEMASQVHPLSRSVPGTALESFRRSGTSLESESDGSTWRSRSHSDELFQPMGSSPSTDSLLMEDVVDSSLWSDLSGDAQELEAESWSLVVDPSFCSKQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCIGNDRNFVINRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQKDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILSKIENKTYTKLKNGHVFRKQALMSKERTLLYEGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNAWMRRIQQAVESCPEEEGGKTSESDEERRRAEVRVAKIQQCQEILSNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKLDPGEPPQAASLLAAALKEAESLQVAVKVSQVSDACQPPEEGCAEPAVTDVLSSRDAAGSPTASLVTEGTGGRGCWDVDPGTQGVGTDQAVSDAGEKVECRSFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQQESVCSGPSFRGSPLQDQEKYRNLEKRREELASIHKLQHQFLQDQQRWHRRCDQQQREQEAKASRLQAREQECQAQEELLLRNREELDQQLQEYQQNLERLREGQRLVERERERMRAQQSLLCGWKHSRQSSLPAAFSPGRMELMELNRSENFCHENSFFINEALVQMSLNTFNKPTPSDATYPPDISHSDLVRTCGNQVALKTDVSRPSDVNHEPWVAAGPCHQIPPLHQSSKDSCENDLDISQTENSPPHDSNPHGPQLQTFIAEAKLSLQRVTTPDGETGDGEENIVYL

Target ORF information:

RefSeq Version	XM_021084443.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021084443.1

ORF Insert Sequence:

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG GTGGTGTGCT CCACCCTGGC CGTTGTTCCG TCCTTCCGTC TCTGTCTCTC 
TCACTGTCTA AATCAGTGTC TCTGCTTCAT CCCTGTAAGC AAAGAGCTTA CAGCTTACCG 
GAGCCACCAA GAGAAAAAAG AATTCAGGAA GAAGAGTGGG ATAAATGCAT CATACCTGCC 
AAATCGGAGT CTGAAAAATA CAAAGTGAGC CGAACATTCA GCTTCCTCAT GAACAGGATG 
ACCAGCCCTC GGAATAAATC TAAGACAAAA GGCAAGGATG CCAAAGACAA AGAGAAACTG 
AGTCGACATC AATTTGTCCC TGGAACATTC TCTGGGGTCC TGCCGTGTTG GGTTTGTGAT 
AAAACTCTCC TGGGGAAAGA GTCGTTCCAG TGTTCCAACT GTAATGCAAA TGTACATAAG 
GGTTGTAAAG ACGCCGCCCC TCCATGTAAC AAGAAATTCC AAGAGAAATA TAACAAGAAC 
AAACCACAGA CCATCCTTGG AAATTCTTCA TTAAGAGACG GCCCACCGCC TGGTCTCTCC 
CTGCACCCTT CCTCCTCTAT GCCTATTGGG TTGCCAACGG GGAGAAAGGA AATGGCCAGC 
CAGGTCCACC CGCTGTCCAG GAGTGTCCCA GGCACCGCCT TGGAAAGCTT CAGGAGGTCG 
GGGACATCCC TGGAGTCTGA GAGTGATGGC AGCACCTGGA GAAGCAGGTC CCACTCTGAT 
GAGCTGTTCC AGCCCATGGG TTCATCTCCC TCCACCGACT CCCTCTTAAT GGAAGACGTC 
GTGGATTCGT CTCTGTGGAG TGACCTCAGT GGTGACGCCC AGGAGTTGGA AGCAGAATCT 
TGGAGTCTGG TGGTGGACCC CTCATTTTGT AGTAAGCAGG AGAAGGATGT CATCAAAAGA 
CAGGACGTCA TTTTTGAGCT GATGCAAACA GAAATGCATC ACATCCAGAC CCTGTTCATC 
ATGTCTGAGA TCTTCAGGAA AGGGATGAAA GAGGAGCTGC AGCTGGACCA CAGCACTGTG 
GATAAAATCT TCCCCTGTTT GGATGAGTTA CTGGAAATCC ACAGGCATTT CTTCTATAGT 
ATGAAGGAGC GAAGGCAAGA ATCCTGTATT GGCAACGACA GGAATTTTGT CATCAACCGC 
ATCGGAGATA TTCTAGTACA ACAGTTTTCA GAAGAAAATG CAAATAAAAT GAAGAAAATA 
TATGGGGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG 
AATAAAAAGT TCCAGAATTT TATTAAGCTC CGAAATAGTA ATCTTTTGGC TCGACGCCGA 
GGAATTCCAG AATGCATTCT GCTGGTCACT CAGCGCATTA CGAAATACCC TGTCTTGGTG 
GAAAGGATAC TGCAGTACAC AAAGGAGAGC ACTGAAGAAC AGAAAGACTT ATGCAAAGCT 
CTTTGCTTAA TTAAAGACAT GATTGCAGCC GTGGATTTAA AAGTCAGCGA GTATGAGAAA 
AAACAAAAAT GGCTTGAGAT CCTAAGTAAG ATCGAAAACA AAACATACAC CAAGCTCAAA 
AACGGCCACG TCTTTCGGAA GCAGGCGCTG ATGAGTAAAG AGAGGACCCT TCTGTATGAG 
GGCCTTGTTT ATTGGAAAAC TGCAACAGGT CGTTTCAAAG ATATCCTAGC TCTGCTTCTA 
ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT 
CAGAAGCCGT CCGTTATTTC CCTTCAGAAG CTCATTGCTA GGGAAGTTGC GAATGAGGAG 
CGAGGAATGT TTCTGATCAG CGCTTCGTCC GCTGGTCCTG AGATGTATGA AATTCATACC 
AACTCCAAGG AGGAACGGAA CGCCTGGATG AGACGGATCC AACAGGCAGT AGAAAGTTGC 
CCAGAAGAAG AAGGGGGAAA GACAAGTGAA TCAGATGAAG AGAGGCGAAG AGCTGAAGTC 
AGAGTGGCCA AAATTCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAAATTTGT 
GCGTATTTGG AGGAGAAGCT GCATATTTAC GCTGAACTTG GAGAACTGAG TGGATTTGAG 
GATGTCCATC TAGAGCCACA CCTCCTCATT AAACTGGACC CCGGAGAGCC TCCCCAGGCG 
GCCTCGTTAC TGGCAGCAGC ACTGAAAGAA GCTGAGAGCC TGCAGGTTGC AGTGAAGGTC 
TCCCAGGTGA GCGATGCGTG TCAGCCCCCC GAGGAAGGCT GTGCAGAACC CGCTGTGACG 
GATGTGCTCA GTTCTCGGGA TGCAGCAGGG TCGCCTACTG CTTCACTAGT CACAGAAGGG 
ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCCGGGACCC AGGGTGTGGG AACCGACCAG 
GCGGTCTCTG ATGCGGGGGA GAAGGTGGAA TGTAGAAGTT TCCCAGGTTC TTCACAGTCC 
GAGATTATAC AAGCGATACA GAATTTAACC CGTCTCTTGT ATAGCCTGCA GGCCGCCTTG 
ACCATCCAGG ACAGCCACAT TGAGATCCAC AAGCTGGTTC TCCAGCAGCA GGAGAGCGTG 
TGTTCTGGCC CTTCTTTCCG AGGCAGCCCC TTGCAGGACC AGGAGAAATA CCGCAATCTG 
GAGAAGCGGC GGGAAGAGCT GGCCAGCATC CACAAGCTGC AGCATCAGTT CCTACAGGAC 
CAGCAGCGCT GGCACCGCAG GTGCGACCAG CAGCAGCGGG AGCAGGAGGC CAAGGCCAGC 
CGGCTGCAAG CACGAGAGCA GGAGTGCCAA GCACAGGAGG AGCTGCTGCT GAGGAATCGG 
GAGGAGCTGG ACCAGCAGCT CCAGGAGTAC CAGCAGAACC TGGAGCGGCT ACGGGAGGGC 
CAACGCCTGG TGGAGAGGGA AAGAGAGAGG ATGCGGGCCC AGCAGAGCCT GCTGTGTGGC 
TGGAAGCACA GCCGGCAGAG TAGCCTTCCT GCAGCATTTT CTCCAGGAAG AATGGAGCTG 
ATGGAACTTA ATCGATCTGA GAATTTCTGT CATGAAAATT CATTCTTCAT CAATGAAGCT 
TTAGTGCAGA TGTCACTTAA CACTTTCAAC AAGCCGACTC CATCAGATGC CACTTACCCC 
CCAGATATAT CCCATTCTGA TTTGGTCAGG ACTTGTGGAA ATCAAGTAGC CCTCAAGACG 
GACGTTTCCC GGCCCTCAGA TGTCAACCAT GAACCGTGGG TAGCTGCTGG GCCCTGTCAT 
CAGATACCCC CTCTCCACCA AAGCAGCAAA GATTCTTGCG AAAATGATTT GGACATCTCT 
CAGACTGAAA ACTCGCCCCC CCATGACTCA AACCCACACG GCCCTCAGCT GCAGACGTTT 
ATAGCAGAAG CAAAGCTAAG TCTACAGAGA GTGACCACAC CAGATGGGGA AACTGGAGAT 
GGAGAAGAAA ACATTGTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_021084441.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020940100.1 Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

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CloneID	OSe244265
Clone ID Related Accession (Same CDS sequence)	XM_021084443.1 , XM_021084441.1
Accession Version	XM_021084441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG GTGGTGTGCT CCACCCTGGC CGTTGTTCCG TCCTTCCGTC TCTGTCTCTC 
TCACTGTCTA AATCAGTGTC TCTGCTTCAT CCCTGTAAGC AAAGAGCTTA CAGCTTACCG 
GAGCCACCAA GAGAAAAAAG AATTCAGGAA GAAGAGTGGG ATAAATGCAT CATACCTGCC 
AAATCGGAGT CTGAAAAATA CAAAGTGAGC CGAACATTCA GCTTCCTCAT GAACAGGATG 
ACCAGCCCTC GGAATAAATC TAAGACAAAA GGCAAGGATG CCAAAGACAA AGAGAAACTG 
AGTCGACATC AATTTGTCCC TGGAACATTC TCTGGGGTCC TGCCGTGTTG GGTTTGTGAT 
AAAACTCTCC TGGGGAAAGA GTCGTTCCAG TGTTCCAACT GTAATGCAAA TGTACATAAG 
GGTTGTAAAG ACGCCGCCCC TCCATGTAAC AAGAAATTCC AAGAGAAATA TAACAAGAAC 
AAACCACAGA CCATCCTTGG AAATTCTTCA TTAAGAGACG GCCCACCGCC TGGTCTCTCC 
CTGCACCCTT CCTCCTCTAT GCCTATTGGG TTGCCAACGG GGAGAAAGGA AATGGCCAGC 
CAGGTCCACC CGCTGTCCAG GAGTGTCCCA GGCACCGCCT TGGAAAGCTT CAGGAGGTCG 
GGGACATCCC TGGAGTCTGA GAGTGATGGC AGCACCTGGA GAAGCAGGTC CCACTCTGAT 
GAGCTGTTCC AGCCCATGGG TTCATCTCCC TCCACCGACT CCCTCTTAAT GGAAGACGTC 
GTGGATTCGT CTCTGTGGAG TGACCTCAGT GGTGACGCCC AGGAGTTGGA AGCAGAATCT 
TGGAGTCTGG TGGTGGACCC CTCATTTTGT AGTAAGCAGG AGAAGGATGT CATCAAAAGA 
CAGGACGTCA TTTTTGAGCT GATGCAAACA GAAATGCATC ACATCCAGAC CCTGTTCATC 
ATGTCTGAGA TCTTCAGGAA AGGGATGAAA GAGGAGCTGC AGCTGGACCA CAGCACTGTG 
GATAAAATCT TCCCCTGTTT GGATGAGTTA CTGGAAATCC ACAGGCATTT CTTCTATAGT 
ATGAAGGAGC GAAGGCAAGA ATCCTGTATT GGCAACGACA GGAATTTTGT CATCAACCGC 
ATCGGAGATA TTCTAGTACA ACAGTTTTCA GAAGAAAATG CAAATAAAAT GAAGAAAATA 
TATGGGGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG 
AATAAAAAGT TCCAGAATTT TATTAAGCTC CGAAATAGTA ATCTTTTGGC TCGACGCCGA 
GGAATTCCAG AATGCATTCT GCTGGTCACT CAGCGCATTA CGAAATACCC TGTCTTGGTG 
GAAAGGATAC TGCAGTACAC AAAGGAGAGC ACTGAAGAAC AGAAAGACTT ATGCAAAGCT 
CTTTGCTTAA TTAAAGACAT GATTGCAGCC GTGGATTTAA AAGTCAGCGA GTATGAGAAA 
AAACAAAAAT GGCTTGAGAT CCTAAGTAAG ATCGAAAACA AAACATACAC CAAGCTCAAA 
AACGGCCACG TCTTTCGGAA GCAGGCGCTG ATGAGTAAAG AGAGGACCCT TCTGTATGAG 
GGCCTTGTTT ATTGGAAAAC TGCAACAGGT CGTTTCAAAG ATATCCTAGC TCTGCTTCTA 
ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT 
CAGAAGCCGT CCGTTATTTC CCTTCAGAAG CTCATTGCTA GGGAAGTTGC GAATGAGGAG 
CGAGGAATGT TTCTGATCAG CGCTTCGTCC GCTGGTCCTG AGATGTATGA AATTCATACC 
AACTCCAAGG AGGAACGGAA CGCCTGGATG AGACGGATCC AACAGGCAGT AGAAAGTTGC 
CCAGAAGAAG AAGGGGGAAA GACAAGTGAA TCAGATGAAG AGAGGCGAAG AGCTGAAGTC 
AGAGTGGCCA AAATTCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAAATTTGT 
GCGTATTTGG AGGAGAAGCT GCATATTTAC GCTGAACTTG GAGAACTGAG TGGATTTGAG 
GATGTCCATC TAGAGCCACA CCTCCTCATT AAACTGGACC CCGGAGAGCC TCCCCAGGCG 
GCCTCGTTAC TGGCAGCAGC ACTGAAAGAA GCTGAGAGCC TGCAGGTTGC AGTGAAGGTC 
TCCCAGGTGA GCGATGCGTG TCAGCCCCCC GAGGAAGGCT GTGCAGAACC CGCTGTGACG 
GATGTGCTCA GTTCTCGGGA TGCAGCAGGG TCGCCTACTG CTTCACTAGT CACAGAAGGG 
ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCCGGGACCC AGGGTGTGGG AACCGACCAG 
GCGGTCTCTG ATGCGGGGGA GAAGGTGGAA TGTAGAAGTT TCCCAGGTTC TTCACAGTCC 
GAGATTATAC AAGCGATACA GAATTTAACC CGTCTCTTGT ATAGCCTGCA GGCCGCCTTG 
ACCATCCAGG ACAGCCACAT TGAGATCCAC AAGCTGGTTC TCCAGCAGCA GGAGAGCGTG 
TGTTCTGGCC CTTCTTTCCG AGGCAGCCCC TTGCAGGACC AGGAGAAATA CCGCAATCTG 
GAGAAGCGGC GGGAAGAGCT GGCCAGCATC CACAAGCTGC AGCATCAGTT CCTACAGGAC 
CAGCAGCGCT GGCACCGCAG GTGCGACCAG CAGCAGCGGG AGCAGGAGGC CAAGGCCAGC 
CGGCTGCAAG CACGAGAGCA GGAGTGCCAA GCACAGGAGG AGCTGCTGCT GAGGAATCGG 
GAGGAGCTGG ACCAGCAGCT CCAGGAGTAC CAGCAGAACC TGGAGCGGCT ACGGGAGGGC 
CAACGCCTGG TGGAGAGGGA AAGAGAGAGG ATGCGGGCCC AGCAGAGCCT GCTGTGTGGC 
TGGAAGCACA GCCGGCAGAG TAGCCTTCCT GCAGCATTTT CTCCAGGAAG AATGGAGCTG 
ATGGAACTTA ATCGATCTGA GAATTTCTGT CATGAAAATT CATTCTTCAT CAATGAAGCT 
TTAGTGCAGA TGTCACTTAA CACTTTCAAC AAGCCGACTC CATCAGATGC CACTTACCCC 
CCAGATATAT CCCATTCTGA TTTGGTCAGG ACTTGTGGAA ATCAAGTAGC CCTCAAGACG 
GACGTTTCCC GGCCCTCAGA TGTCAACCAT GAACCGTGGG TAGCTGCTGG GCCCTGTCAT 
CAGATACCCC CTCTCCACCA AAGCAGCAAA GATTCTTGCG AAAATGATTT GGACATCTCT 
CAGACTGAAA ACTCGCCCCC CCATGACTCA AACCCACACG GCCCTCAGCT GCAGACGTTT 
ATAGCAGAAG CAAAGCTAAG TCTACAGAGA GTGACCACAC CAGATGGGGA AACTGGAGAT 
GGAGAAGAAA ACATTGTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020940100.1
CDS	288..5474
Translation	MELNCSEVPLYGQMTVYAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTADNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSDAVTYVDNLACRLARLLVTQADRLTASSHQTLLTPFALTEGALPALDEELVLALTHLELPVGWTVLGNSSLEVSLHRESLLHLAVRWGLTKLSQFLLCLPGGIQALALPNEEGATPIDLALRGGHSKLVEDLTNFQGRRSPGFSRVQLSEGASLQYIHSSETLTLTLNHTADHLVEADIKLFRKYFWDRAFLIKALEEQEARSEEKLDMPSSAAESEEEIKNLVSSRSSPGKESVKCVESPVVQLNEGEGQDGLDLDRFSDVLRKSKRPPLLLAAGRPSDMLNGGDEVYANCMVIDQVGDLDVNYVNIEGITPDTSPESMGSTLGSESSKHILPTETSSGMYPPHGNKDVILHPEAQRSFMSPSSSYASNLNLSFGLHGFEKEQSHLKRRSSSLDALDADSEGEGHSAQSHLCHTPVSQSSSRTGLPSGDELDSFETNTEPDFNISRTESLSLSSSLQLKESLLSGVRSRSYSCSSPKISLGKTRLIRDFTVCNSVEGGVLHPGRCSVLPSLSLSLSKSVSLLHPCKQRAYSLPEPPREKRIQEEEWDKCIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLSRHQFVPGTFSGVLPCWVCDKTLLGKESFQCSNCNANVHKGCKDAAPPCNKKFQEKYNKNKPQTILGNSSLRDGPPPGLSLHPSSSMPIGLPTGRKEMASQVHPLSRSVPGTALESFRRSGTSLESESDGSTWRSRSHSDELFQPMGSSPSTDSLLMEDVVDSSLWSDLSGDAQELEAESWSLVVDPSFCSKQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCIGNDRNFVINRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQKDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILSKIENKTYTKLKNGHVFRKQALMSKERTLLYEGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNAWMRRIQQAVESCPEEEGGKTSESDEERRRAEVRVAKIQQCQEILSNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKLDPGEPPQAASLLAAALKEAESLQVAVKVSQVSDACQPPEEGCAEPAVTDVLSSRDAAGSPTASLVTEGTGGRGCWDVDPGTQGVGTDQAVSDAGEKVECRSFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQQESVCSGPSFRGSPLQDQEKYRNLEKRREELASIHKLQHQFLQDQQRWHRRCDQQQREQEAKASRLQAREQECQAQEELLLRNREELDQQLQEYQQNLERLREGQRLVERERERMRAQQSLLCGWKHSRQSSLPAAFSPGRMELMELNRSENFCHENSFFINEALVQMSLNTFNKPTPSDATYPPDISHSDLVRTCGNQVALKTDVSRPSDVNHEPWVAAGPCHQIPPLHQSSKDSCENDLDISQTENSPPHDSNPHGPQLQTFIAEAKLSLQRVTTPDGETGDGEENIVYL

Target ORF information:

RefSeq Version	XM_021084441.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021084441.1

ORF Insert Sequence:

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG GTGGTGTGCT CCACCCTGGC CGTTGTTCCG TCCTTCCGTC TCTGTCTCTC 
TCACTGTCTA AATCAGTGTC TCTGCTTCAT CCCTGTAAGC AAAGAGCTTA CAGCTTACCG 
GAGCCACCAA GAGAAAAAAG AATTCAGGAA GAAGAGTGGG ATAAATGCAT CATACCTGCC 
AAATCGGAGT CTGAAAAATA CAAAGTGAGC CGAACATTCA GCTTCCTCAT GAACAGGATG 
ACCAGCCCTC GGAATAAATC TAAGACAAAA GGCAAGGATG CCAAAGACAA AGAGAAACTG 
AGTCGACATC AATTTGTCCC TGGAACATTC TCTGGGGTCC TGCCGTGTTG GGTTTGTGAT 
AAAACTCTCC TGGGGAAAGA GTCGTTCCAG TGTTCCAACT GTAATGCAAA TGTACATAAG 
GGTTGTAAAG ACGCCGCCCC TCCATGTAAC AAGAAATTCC AAGAGAAATA TAACAAGAAC 
AAACCACAGA CCATCCTTGG AAATTCTTCA TTAAGAGACG GCCCACCGCC TGGTCTCTCC 
CTGCACCCTT CCTCCTCTAT GCCTATTGGG TTGCCAACGG GGAGAAAGGA AATGGCCAGC 
CAGGTCCACC CGCTGTCCAG GAGTGTCCCA GGCACCGCCT TGGAAAGCTT CAGGAGGTCG 
GGGACATCCC TGGAGTCTGA GAGTGATGGC AGCACCTGGA GAAGCAGGTC CCACTCTGAT 
GAGCTGTTCC AGCCCATGGG TTCATCTCCC TCCACCGACT CCCTCTTAAT GGAAGACGTC 
GTGGATTCGT CTCTGTGGAG TGACCTCAGT GGTGACGCCC AGGAGTTGGA AGCAGAATCT 
TGGAGTCTGG TGGTGGACCC CTCATTTTGT AGTAAGCAGG AGAAGGATGT CATCAAAAGA 
CAGGACGTCA TTTTTGAGCT GATGCAAACA GAAATGCATC ACATCCAGAC CCTGTTCATC 
ATGTCTGAGA TCTTCAGGAA AGGGATGAAA GAGGAGCTGC AGCTGGACCA CAGCACTGTG 
GATAAAATCT TCCCCTGTTT GGATGAGTTA CTGGAAATCC ACAGGCATTT CTTCTATAGT 
ATGAAGGAGC GAAGGCAAGA ATCCTGTATT GGCAACGACA GGAATTTTGT CATCAACCGC 
ATCGGAGATA TTCTAGTACA ACAGTTTTCA GAAGAAAATG CAAATAAAAT GAAGAAAATA 
TATGGGGAAT TCTGTAGCCA TCACAAAGAA GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG 
AATAAAAAGT TCCAGAATTT TATTAAGCTC CGAAATAGTA ATCTTTTGGC TCGACGCCGA 
GGAATTCCAG AATGCATTCT GCTGGTCACT CAGCGCATTA CGAAATACCC TGTCTTGGTG 
GAAAGGATAC TGCAGTACAC AAAGGAGAGC ACTGAAGAAC AGAAAGACTT ATGCAAAGCT 
CTTTGCTTAA TTAAAGACAT GATTGCAGCC GTGGATTTAA AAGTCAGCGA GTATGAGAAA 
AAACAAAAAT GGCTTGAGAT CCTAAGTAAG ATCGAAAACA AAACATACAC CAAGCTCAAA 
AACGGCCACG TCTTTCGGAA GCAGGCGCTG ATGAGTAAAG AGAGGACCCT TCTGTATGAG 
GGCCTTGTTT ATTGGAAAAC TGCAACAGGT CGTTTCAAAG ATATCCTAGC TCTGCTTCTA 
ACTGATGTGC TGCTCTTTTT ACAAGAAAAA GACCAGAAAT ACATCTTTGC AGCTGTTGAT 
CAGAAGCCGT CCGTTATTTC CCTTCAGAAG CTCATTGCTA GGGAAGTTGC GAATGAGGAG 
CGAGGAATGT TTCTGATCAG CGCTTCGTCC GCTGGTCCTG AGATGTATGA AATTCATACC 
AACTCCAAGG AGGAACGGAA CGCCTGGATG AGACGGATCC AACAGGCAGT AGAAAGTTGC 
CCAGAAGAAG AAGGGGGAAA GACAAGTGAA TCAGATGAAG AGAGGCGAAG AGCTGAAGTC 
AGAGTGGCCA AAATTCAGCA ATGTCAAGAA ATACTCAGTA ACCAAGACCA ACAAATTTGT 
GCGTATTTGG AGGAGAAGCT GCATATTTAC GCTGAACTTG GAGAACTGAG TGGATTTGAG 
GATGTCCATC TAGAGCCACA CCTCCTCATT AAACTGGACC CCGGAGAGCC TCCCCAGGCG 
GCCTCGTTAC TGGCAGCAGC ACTGAAAGAA GCTGAGAGCC TGCAGGTTGC AGTGAAGGTC 
TCCCAGGTGA GCGATGCGTG TCAGCCCCCC GAGGAAGGCT GTGCAGAACC CGCTGTGACG 
GATGTGCTCA GTTCTCGGGA TGCAGCAGGG TCGCCTACTG CTTCACTAGT CACAGAAGGG 
ACAGGAGGAA GAGGCTGTTG GGATGTGGAT CCCGGGACCC AGGGTGTGGG AACCGACCAG 
GCGGTCTCTG ATGCGGGGGA GAAGGTGGAA TGTAGAAGTT TCCCAGGTTC TTCACAGTCC 
GAGATTATAC AAGCGATACA GAATTTAACC CGTCTCTTGT ATAGCCTGCA GGCCGCCTTG 
ACCATCCAGG ACAGCCACAT TGAGATCCAC AAGCTGGTTC TCCAGCAGCA GGAGAGCGTG 
TGTTCTGGCC CTTCTTTCCG AGGCAGCCCC TTGCAGGACC AGGAGAAATA CCGCAATCTG 
GAGAAGCGGC GGGAAGAGCT GGCCAGCATC CACAAGCTGC AGCATCAGTT CCTACAGGAC 
CAGCAGCGCT GGCACCGCAG GTGCGACCAG CAGCAGCGGG AGCAGGAGGC CAAGGCCAGC 
CGGCTGCAAG CACGAGAGCA GGAGTGCCAA GCACAGGAGG AGCTGCTGCT GAGGAATCGG 
GAGGAGCTGG ACCAGCAGCT CCAGGAGTAC CAGCAGAACC TGGAGCGGCT ACGGGAGGGC 
CAACGCCTGG TGGAGAGGGA AAGAGAGAGG ATGCGGGCCC AGCAGAGCCT GCTGTGTGGC 
TGGAAGCACA GCCGGCAGAG TAGCCTTCCT GCAGCATTTT CTCCAGGAAG AATGGAGCTG 
ATGGAACTTA ATCGATCTGA GAATTTCTGT CATGAAAATT CATTCTTCAT CAATGAAGCT 
TTAGTGCAGA TGTCACTTAA CACTTTCAAC AAGCCGACTC CATCAGATGC CACTTACCCC 
CCAGATATAT CCCATTCTGA TTTGGTCAGG ACTTGTGGAA ATCAAGTAGC CCTCAAGACG 
GACGTTTCCC GGCCCTCAGA TGTCAACCAT GAACCGTGGG TAGCTGCTGG GCCCTGTCAT 
CAGATACCCC CTCTCCACCA AAGCAGCAAA GATTCTTGCG AAAATGATTT GGACATCTCT 
CAGACTGAAA ACTCGCCCCC CCATGACTCA AACCCACACG GCCCTCAGCT GCAGACGTTT 
ATAGCAGAAG CAAAGCTAAG TCTACAGAGA GTGACCACAC CAGATGGGGA AACTGGAGAT 
GGAGAAGAAA ACATTGTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_021084442.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020940101.1 Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

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CloneID	OSe244266
Clone ID Related Accession (Same CDS sequence)	XM_021084442.1
Accession Version	XM_021084442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG AGCAAAGAGC TTACAGCTTA CCGGAGCCAC CAAGAGAAAA AAGAATTCAG 
GAAGAAGAGT GGGATAAATG CATCATACCT GCCAAATCGG AGTCTGAAAA ATACAAAGTG 
AGCCGAACAT TCAGCTTCCT CATGAACAGG ATGACCAGCC CTCGGAATAA ATCTAAGACA 
AAAGGCAAGG ATGCCAAAGA CAAAGAGAAA CTGAGTCGAC ATCAATTTGT CCCTGGAACA 
TTCTCTGGGG TCCTGCCGTG TTGGGTTTGT GATAAAACTC TCCTGGGGAA AGAGTCGTTC 
CAGTGTTCCA ACTGTAATGC AAATGTACAT AAGGGTTGTA AAGACGCCGC CCCTCCATGT 
AACAAGAAAT TCCAAGAGAA ATATAACAAG AACAAACCAC AGACCATCCT TGGAAATTCT 
TCATTAAGAG ACGGCCCACC GCCTGGTCTC TCCCTGCACC CTTCCTCCTC TATGCCTATT 
GGGTTGCCAA CGGGGAGAAA GGAAATGGCC AGCCAGGTCC ACCCGCTGTC CAGGAGTGTC 
CCAGGCACCG CCTTGGAAAG CTTCAGGAGG TCGGGGACAT CCCTGGAGTC TGAGAGTGAT 
GGCAGCACCT GGAGAAGCAG GTCCCACTCT GATGAGCTGT TCCAGCCCAT GGGTTCATCT 
CCCTCCACCG ACTCCCTCTT AATGGAAGAC GTCGTGGATT CGTCTCTGTG GAGTGACCTC 
AGTGGTGACG CCCAGGAGTT GGAAGCAGAA TCTTGGAGTC TGGTGGTGGA CCCCTCATTT 
TGTAGTAAGC AGGAGAAGGA TGTCATCAAA AGACAGGACG TCATTTTTGA GCTGATGCAA 
ACAGAAATGC ATCACATCCA GACCCTGTTC ATCATGTCTG AGATCTTCAG GAAAGGGATG 
AAAGAGGAGC TGCAGCTGGA CCACAGCACT GTGGATAAAA TCTTCCCCTG TTTGGATGAG 
TTACTGGAAA TCCACAGGCA TTTCTTCTAT AGTATGAAGG AGCGAAGGCA AGAATCCTGT 
ATTGGCAACG ACAGGAATTT TGTCATCAAC CGCATCGGAG ATATTCTAGT ACAACAGTTT 
TCAGAAGAAA ATGCAAATAA AATGAAGAAA ATATATGGGG AATTCTGTAG CCATCACAAA 
GAAGCTGTTA GCCTCTTTAA AGAACTCCAG CAGAATAAAA AGTTCCAGAA TTTTATTAAG 
CTCCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATTC CAGAATGCAT TCTGCTGGTC 
ACTCAGCGCA TTACGAAATA CCCTGTCTTG GTGGAAAGGA TACTGCAGTA CACAAAGGAG 
AGCACTGAAG AACAGAAAGA CTTATGCAAA GCTCTTTGCT TAATTAAAGA CATGATTGCA 
GCCGTGGATT TAAAAGTCAG CGAGTATGAG AAAAAACAAA AATGGCTTGA GATCCTAAGT 
AAGATCGAAA ACAAAACATA CACCAAGCTC AAAAACGGCC ACGTCTTTCG GAAGCAGGCG 
CTGATGAGTA AAGAGAGGAC CCTTCTGTAT GAGGGCCTTG TTTATTGGAA AACTGCAACA 
GGTCGTTTCA AAGATATCCT AGCTCTGCTT CTAACTGATG TGCTGCTCTT TTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CGTCCGTTAT TTCCCTTCAG 
AAGCTCATTG CTAGGGAAGT TGCGAATGAG GAGCGAGGAA TGTTTCTGAT CAGCGCTTCG 
TCCGCTGGTC CTGAGATGTA TGAAATTCAT ACCAACTCCA AGGAGGAACG GAACGCCTGG 
ATGAGACGGA TCCAACAGGC AGTAGAAAGT TGCCCAGAAG AAGAAGGGGG AAAGACAAGT 
GAATCAGATG AAGAGAGGCG AAGAGCTGAA GTCAGAGTGG CCAAAATTCA GCAATGTCAA 
GAAATACTCA GTAACCAAGA CCAACAAATT TGTGCGTATT TGGAGGAGAA GCTGCATATT 
TACGCTGAAC TTGGAGAACT GAGTGGATTT GAGGATGTCC ATCTAGAGCC ACACCTCCTC 
ATTAAACTGG ACCCCGGAGA GCCTCCCCAG GCGGCCTCGT TACTGGCAGC AGCACTGAAA 
GAAGCTGAGA GCCTGCAGGT TGCAGTGAAG GTCTCCCAGG TGAGCGATGC GTGTCAGCCC 
CCCGAGGAAG GCTGTGCAGA ACCCGCTGTG ACGGATGTGC TCAGTTCTCG GGATGCAGCA 
GGGTCGCCTA CTGCTTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG 
GATCCCGGGA CCCAGGGTGT GGGAACCGAC CAGGCGGTCT CTGATGCGGG GGAGAAGGTG 
GAATGTAGAA GTTTCCCAGG TTCTTCACAG TCCGAGATTA TACAAGCGAT ACAGAATTTA 
ACCCGTCTCT TGTATAGCCT GCAGGCCGCC TTGACCATCC AGGACAGCCA CATTGAGATC 
CACAAGCTGG TTCTCCAGCA GCAGGAGAGC GTGTGTTCTG GCCCTTCTTT CCGAGGCAGC 
CCCTTGCAGG ACCAGGAGAA ATACCGCAAT CTGGAGAAGC GGCGGGAAGA GCTGGCCAGC 
ATCCACAAGC TGCAGCATCA GTTCCTACAG GACCAGCAGC GCTGGCACCG CAGGTGCGAC 
CAGCAGCAGC GGGAGCAGGA GGCCAAGGCC AGCCGGCTGC AAGCACGAGA GCAGGAGTGC 
CAAGCACAGG AGGAGCTGCT GCTGAGGAAT CGGGAGGAGC TGGACCAGCA GCTCCAGGAG 
TACCAGCAGA ACCTGGAGCG GCTACGGGAG GGCCAACGCC TGGTGGAGAG GGAAAGAGAG 
AGGATGCGGG CCCAGCAGAG CCTGCTGTGT GGCTGGAAGC ACAGCCGGCA GAGTAGCCTT 
CCTGCAGCAT TTTCTCCAGG AAGAATGGAG CTGATGGAAC TTAATCGATC TGAGAATTTC 
TGTCATGAAA ATTCATTCTT CATCAATGAA GCTTTAGTGC AGATGTCACT TAACACTTTC 
AACAAGCCGA CTCCATCAGA TGCCACTTAC CCCCCAGATA TATCCCATTC TGATTTGGTC 
AGGACTTGTG GAAATCAAGT AGCCCTCAAG ACGGACGTTT CCCGGCCCTC AGATGTCAAC 
CATGAACCGT GGGTAGCTGC TGGGCCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAAGATTCTT GCGAAAATGA TTTGGACATC TCTCAGACTG AAAACTCGCC CCCCCATGAC 
TCAAACCCAC ACGGCCCTCA GCTGCAGACG TTTATAGCAG AAGCAAAGCT AAGTCTACAG 
AGAGTGACCA CACCAGATGG GGAAACTGGA GATGGAGAAG AAAACATTGT TTACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020940101.1
CDS	288..5387
Translation	MELNCSEVPLYGQMTVYAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTADNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSDAVTYVDNLACRLARLLVTQADRLTASSHQTLLTPFALTEGALPALDEELVLALTHLELPVGWTVLGNSSLEVSLHRESLLHLAVRWGLTKLSQFLLCLPGGIQALALPNEEGATPIDLALRGGHSKLVEDLTNFQGRRSPGFSRVQLSEGASLQYIHSSETLTLTLNHTADHLVEADIKLFRKYFWDRAFLIKALEEQEARSEEKLDMPSSAAESEEEIKNLVSSRSSPGKESVKCVESPVVQLNEGEGQDGLDLDRFSDVLRKSKRPPLLLAAGRPSDMLNGGDEVYANCMVIDQVGDLDVNYVNIEGITPDTSPESMGSTLGSESSKHILPTETSSGMYPPHGNKDVILHPEAQRSFMSPSSSYASNLNLSFGLHGFEKEQSHLKRRSSSLDALDADSEGEGHSAQSHLCHTPVSQSSSRTGLPSGDELDSFETNTEPDFNISRTESLSLSSSLQLKESLLSGVRSRSYSCSSPKISLGKTRLIRDFTVCNSVEEQRAYSLPEPPREKRIQEEEWDKCIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLSRHQFVPGTFSGVLPCWVCDKTLLGKESFQCSNCNANVHKGCKDAAPPCNKKFQEKYNKNKPQTILGNSSLRDGPPPGLSLHPSSSMPIGLPTGRKEMASQVHPLSRSVPGTALESFRRSGTSLESESDGSTWRSRSHSDELFQPMGSSPSTDSLLMEDVVDSSLWSDLSGDAQELEAESWSLVVDPSFCSKQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCIGNDRNFVINRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQKDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILSKIENKTYTKLKNGHVFRKQALMSKERTLLYEGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNAWMRRIQQAVESCPEEEGGKTSESDEERRRAEVRVAKIQQCQEILSNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKLDPGEPPQAASLLAAALKEAESLQVAVKVSQVSDACQPPEEGCAEPAVTDVLSSRDAAGSPTASLVTEGTGGRGCWDVDPGTQGVGTDQAVSDAGEKVECRSFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQQESVCSGPSFRGSPLQDQEKYRNLEKRREELASIHKLQHQFLQDQQRWHRRCDQQQREQEAKASRLQAREQECQAQEELLLRNREELDQQLQEYQQNLERLREGQRLVERERERMRAQQSLLCGWKHSRQSSLPAAFSPGRMELMELNRSENFCHENSFFINEALVQMSLNTFNKPTPSDATYPPDISHSDLVRTCGNQVALKTDVSRPSDVNHEPWVAAGPCHQIPPLHQSSKDSCENDLDISQTENSPPHDSNPHGPQLQTFIAEAKLSLQRVTTPDGETGDGEENIVYL

Target ORF information:

RefSeq Version	XM_021084442.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021084442.1

ORF Insert Sequence:

ATGGAGTTGA ACTGCAGCGA AGTACCTCTT TATGGGCAGA TGACAGTCTA CGCGAAGTTT 
GGCAAAAATG TCTATCTTCC TAAAGATGCT GAGTTCTACT TTATTTACAA TGGATCTCAT 
CAGAGGCACA TTGTGATTGC AGAGCGCACG GCAGATAACG TTCTCCAGTC CAGTGTTCCA 
GGCCATGGGC TGCAGGAGAC GGTGACGGTG TCCGTGTGCC TCTGCTCGGA GGGTTACTCC 
CCGGTGACCA TGGGCTCCGA TGCTGTGACG TATGTGGACA ACCTGGCCTG CAGGCTGGCT 
CGCTTGCTTG TCACCCAAGC CGATCGCCTC ACAGCCAGTA GCCATCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGAGGG GGCGCTGCCT GCTCTGGACG AGGAGCTAGT GCTGGCCCTG 
ACCCATCTGG AGTTGCCAGT TGGATGGACT GTCTTGGGAA ATTCTTCACT TGAAGTTTCT 
CTCCACAGAG AGTCGCTGCT GCATCTGGCT GTGAGGTGGG GCCTCACCAA GCTTTCCCAG 
TTCCTCCTGT GTCTCCCTGG GGGAATCCAG GCCTTGGCTT TACCCAACGA GGAGGGGGCC 
ACGCCAATAG ACTTAGCTTT ACGGGGCGGA CACTCCAAGC TGGTTGAAGA CCTCACAAAC 
TTCCAGGGCA GACGTTCCCC AGGCTTCTCC CGAGTACAGC TCAGCGAAGG TGCCTCCCTG 
CAGTATATTC ACTCGTCAGA AACACTGACC CTGACGCTGA ACCACACAGC CGACCATTTG 
GTGGAGGCGG ATATTAAACT CTTCAGGAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGC CAGGTCCGAG GAAAAACTAG ATATGCCCTC CAGCGCTGCA 
GAAAGCGAAG AAGAGATTAA GAATTTGGTG TCCAGCAGAT CATCGCCTGG AAAGGAGAGT 
GTAAAGTGTG TGGAAAGCCC GGTGGTTCAA CTGAATGAAG GTGAAGGCCA GGACGGTCTA 
GATTTGGATC GTTTCTCTGA TGTCCTCAGA AAATCCAAGC GTCCTCCCCT GCTCCTTGCT 
GCTGGCCGTC CTTCTGACAT GCTGAATGGC GGTGATGAAG TTTATGCTAA CTGCATGGTG 
ATAGATCAGG TTGGTGATCT GGATGTTAAC TATGTTAATA TAGAGGGAAT CACTCCGGAT 
ACCAGCCCTG AATCCATGGG TTCTACTCTG GGGTCTGAGA GCAGCAAGCA CATCCTTCCT 
ACTGAAACCA GCTCTGGCAT GTACCCCCCA CATGGGAACA AGGACGTGAT ACTCCACCCT 
GAAGCCCAGC GGTCTTTCAT GTCACCATCT AGCTCATATG CTTCCAATTT GAATCTTTCT 
TTCGGTCTTC ATGGATTTGA AAAGGAACAA AGTCATCTAA AGAGAAGAAG CTCCAGTCTT 
GATGCCCTGG ATGCCGACAG TGAGGGAGAG GGGCACTCCG CCCAGTCGCA CCTTTGCCAC 
ACTCCAGTGT CTCAGAGTTC TTCGAGGACT GGCCTTCCTA GTGGGGATGA ACTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CTGAATCACT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCACTGCTT TCTGGAGTCC GCTCACGTTC TTATTCCTGC 
TCATCACCTA AAATTTCTTT AGGGAAAACG CGATTGATAC GTGATTTTAC AGTGTGCAAT 
TCAGTTGAAG AGCAAAGAGC TTACAGCTTA CCGGAGCCAC CAAGAGAAAA AAGAATTCAG 
GAAGAAGAGT GGGATAAATG CATCATACCT GCCAAATCGG AGTCTGAAAA ATACAAAGTG 
AGCCGAACAT TCAGCTTCCT CATGAACAGG ATGACCAGCC CTCGGAATAA ATCTAAGACA 
AAAGGCAAGG ATGCCAAAGA CAAAGAGAAA CTGAGTCGAC ATCAATTTGT CCCTGGAACA 
TTCTCTGGGG TCCTGCCGTG TTGGGTTTGT GATAAAACTC TCCTGGGGAA AGAGTCGTTC 
CAGTGTTCCA ACTGTAATGC AAATGTACAT AAGGGTTGTA AAGACGCCGC CCCTCCATGT 
AACAAGAAAT TCCAAGAGAA ATATAACAAG AACAAACCAC AGACCATCCT TGGAAATTCT 
TCATTAAGAG ACGGCCCACC GCCTGGTCTC TCCCTGCACC CTTCCTCCTC TATGCCTATT 
GGGTTGCCAA CGGGGAGAAA GGAAATGGCC AGCCAGGTCC ACCCGCTGTC CAGGAGTGTC 
CCAGGCACCG CCTTGGAAAG CTTCAGGAGG TCGGGGACAT CCCTGGAGTC TGAGAGTGAT 
GGCAGCACCT GGAGAAGCAG GTCCCACTCT GATGAGCTGT TCCAGCCCAT GGGTTCATCT 
CCCTCCACCG ACTCCCTCTT AATGGAAGAC GTCGTGGATT CGTCTCTGTG GAGTGACCTC 
AGTGGTGACG CCCAGGAGTT GGAAGCAGAA TCTTGGAGTC TGGTGGTGGA CCCCTCATTT 
TGTAGTAAGC AGGAGAAGGA TGTCATCAAA AGACAGGACG TCATTTTTGA GCTGATGCAA 
ACAGAAATGC ATCACATCCA GACCCTGTTC ATCATGTCTG AGATCTTCAG GAAAGGGATG 
AAAGAGGAGC TGCAGCTGGA CCACAGCACT GTGGATAAAA TCTTCCCCTG TTTGGATGAG 
TTACTGGAAA TCCACAGGCA TTTCTTCTAT AGTATGAAGG AGCGAAGGCA AGAATCCTGT 
ATTGGCAACG ACAGGAATTT TGTCATCAAC CGCATCGGAG ATATTCTAGT ACAACAGTTT 
TCAGAAGAAA ATGCAAATAA AATGAAGAAA ATATATGGGG AATTCTGTAG CCATCACAAA 
GAAGCTGTTA GCCTCTTTAA AGAACTCCAG CAGAATAAAA AGTTCCAGAA TTTTATTAAG 
CTCCGAAATA GTAATCTTTT GGCTCGACGC CGAGGAATTC CAGAATGCAT TCTGCTGGTC 
ACTCAGCGCA TTACGAAATA CCCTGTCTTG GTGGAAAGGA TACTGCAGTA CACAAAGGAG 
AGCACTGAAG AACAGAAAGA CTTATGCAAA GCTCTTTGCT TAATTAAAGA CATGATTGCA 
GCCGTGGATT TAAAAGTCAG CGAGTATGAG AAAAAACAAA AATGGCTTGA GATCCTAAGT 
AAGATCGAAA ACAAAACATA CACCAAGCTC AAAAACGGCC ACGTCTTTCG GAAGCAGGCG 
CTGATGAGTA AAGAGAGGAC CCTTCTGTAT GAGGGCCTTG TTTATTGGAA AACTGCAACA 
GGTCGTTTCA AAGATATCCT AGCTCTGCTT CTAACTGATG TGCTGCTCTT TTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CGTCCGTTAT TTCCCTTCAG 
AAGCTCATTG CTAGGGAAGT TGCGAATGAG GAGCGAGGAA TGTTTCTGAT CAGCGCTTCG 
TCCGCTGGTC CTGAGATGTA TGAAATTCAT ACCAACTCCA AGGAGGAACG GAACGCCTGG 
ATGAGACGGA TCCAACAGGC AGTAGAAAGT TGCCCAGAAG AAGAAGGGGG AAAGACAAGT 
GAATCAGATG AAGAGAGGCG AAGAGCTGAA GTCAGAGTGG CCAAAATTCA GCAATGTCAA 
GAAATACTCA GTAACCAAGA CCAACAAATT TGTGCGTATT TGGAGGAGAA GCTGCATATT 
TACGCTGAAC TTGGAGAACT GAGTGGATTT GAGGATGTCC ATCTAGAGCC ACACCTCCTC 
ATTAAACTGG ACCCCGGAGA GCCTCCCCAG GCGGCCTCGT TACTGGCAGC AGCACTGAAA 
GAAGCTGAGA GCCTGCAGGT TGCAGTGAAG GTCTCCCAGG TGAGCGATGC GTGTCAGCCC 
CCCGAGGAAG GCTGTGCAGA ACCCGCTGTG ACGGATGTGC TCAGTTCTCG GGATGCAGCA 
GGGTCGCCTA CTGCTTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG 
GATCCCGGGA CCCAGGGTGT GGGAACCGAC CAGGCGGTCT CTGATGCGGG GGAGAAGGTG 
GAATGTAGAA GTTTCCCAGG TTCTTCACAG TCCGAGATTA TACAAGCGAT ACAGAATTTA 
ACCCGTCTCT TGTATAGCCT GCAGGCCGCC TTGACCATCC AGGACAGCCA CATTGAGATC 
CACAAGCTGG TTCTCCAGCA GCAGGAGAGC GTGTGTTCTG GCCCTTCTTT CCGAGGCAGC 
CCCTTGCAGG ACCAGGAGAA ATACCGCAAT CTGGAGAAGC GGCGGGAAGA GCTGGCCAGC 
ATCCACAAGC TGCAGCATCA GTTCCTACAG GACCAGCAGC GCTGGCACCG CAGGTGCGAC 
CAGCAGCAGC GGGAGCAGGA GGCCAAGGCC AGCCGGCTGC AAGCACGAGA GCAGGAGTGC 
CAAGCACAGG AGGAGCTGCT GCTGAGGAAT CGGGAGGAGC TGGACCAGCA GCTCCAGGAG 
TACCAGCAGA ACCTGGAGCG GCTACGGGAG GGCCAACGCC TGGTGGAGAG GGAAAGAGAG 
AGGATGCGGG CCCAGCAGAG CCTGCTGTGT GGCTGGAAGC ACAGCCGGCA GAGTAGCCTT 
CCTGCAGCAT TTTCTCCAGG AAGAATGGAG CTGATGGAAC TTAATCGATC TGAGAATTTC 
TGTCATGAAA ATTCATTCTT CATCAATGAA GCTTTAGTGC AGATGTCACT TAACACTTTC 
AACAAGCCGA CTCCATCAGA TGCCACTTAC CCCCCAGATA TATCCCATTC TGATTTGGTC 
AGGACTTGTG GAAATCAAGT AGCCCTCAAG ACGGACGTTT CCCGGCCCTC AGATGTCAAC 
CATGAACCGT GGGTAGCTGC TGGGCCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAAGATTCTT GCGAAAATGA TTTGGACATC TCTCAGACTG AAAACTCGCC CCCCCATGAC 
TCAAACCCAC ACGGCCCTCA GCTGCAGACG TTTATAGCAG AAGCAAAGCT AAGTCTACAG 
AGAGTGACCA CACCAGATGG GGAAACTGGA GATGGAGAAG AAAACATTGT TTACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

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ARHGEF28 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム