Adi3 cDNA ORF clone, Solanum lycopersicum(Lycopersicon esculentum)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adi3
Entrez Gene ID	543952
Full Name	AvrPto-dependent Pto-interacting protein 3
Gene Type	protein-coding
Organism	Solanum lycopersicum(Lycopersicon esculentum)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001247682.2	NP_001234611.1	AvrPto-dependent Pto-interacting protein 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Pto kinase binds two domains of AvrPtoB and its proximity to the effector E3 ligase determines if it evades degradation and activates plant immunity.
Mathieu J, Schwizer S, Martin GB
PLoS pathogens10(7)e1004227(2014 Jul)

The tomato cell death suppressor Adi3 is restricted to the endosomal system in response to the Pseudomonas syringae effector protein AvrPto.
Ek-Ramos MJ, Avila J, Nelson Dittrich AC, Su D, Gray JW, Devarenne TP
PloS one9(10)e110807(2014)

Ubiquitination of the tomato cell death suppressor Adi3 by the RING E3 ubiquitin ligase AdBiL.
Avila J, Devarenne TP
Biochemical and biophysical research communications430(1)119-24(2013 Jan)

The ?-subunit of the SnRK1 complex is phosphorylated by the plant cell death suppressor Adi3.
Avila J, Gregory OG, Su D, Deeter TA, Chen S, Silva-Sanchez C, Xu S, Martin GB, Devarenne TP
Plant physiology159(3)1277-90(2012 Jul)

An ATP analog-sensitive version of the tomato cell death suppressor protein kinase Adi3 for use in substrate identification.
Dittrich AC, Devarenne TP
Biochimica et biophysica acta1824(2)269-73(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that the ability of Pto to interact with a second site distal to the E3 ligase domain in AvrPtoB, and not a higher kinase activity or T450 phosphorylation, allows Pto to evade ubiquitination and to confer immunity to Pst.
Title: Pto kinase binds two domains of AvrPtoB and its proximity to the effector E3 ligase determines if it evades degradation and activates plant immunity.

inhibiting nuclear import and constraining Adi3 to the endosomal system in response to AvrPto is a mechanism to initiate the cell death associated with resistance
Title: The tomato cell death suppressor Adi3 is restricted to the endosomal system in response to the Pseudomonas syringae effector protein AvrPto.

AdBiL is an active E3 ubiquitin ligase using the E2 ubiquitin ligase UBC8 to ubiquitinate Adi3.
Title: Ubiquitination of the tomato cell death suppressor Adi3 by the RING E3 ubiquitin ligase AdBiL.

Adi3 is capable of suppressing the kinase activity of the SnRK1 complex through Gal83 phosphorylation plus the interaction with SnRK1 and suggested that this function may be related to the cell death suppression activity of Adi3. [Adi3]
Title: The ?-subunit of the SnRK1 complex is phosphorylated by the plant cell death suppressor Adi3.

Met385 was identified as the "gatekeeper" residue and the M385G mutation allows for the use of two bulky ATP analogs.
Title: An ATP analog-sensitive version of the tomato cell death suppressor protein kinase Adi3 for use in substrate identification.

The following Adi3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adi3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSo108555	NM_001247682.2 Latest version!	Solanum lycopersicum AvrPto-dependent Pto-interacting protein 3 (Adi3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adi3 ( NM_001247682.2 ) cDNA ORF clone, Solanum lycopersicum(Lycopersicon esculentum) -> NP_001234611.1 Solanum lycopersicum AvrPto-dependent Pto-interacting protein 3 (Adi3), mRNA.

Price & Availability↑

CloneID	OSo108555
Clone ID Related Accession (Same CDS sequence)	NM_001247682.2
Accession Version	NM_001247682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-25
Organism	Solanum lycopersicum(Lycopersicon esculentum)
Product	AvrPto-dependent Pto-interacting protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB690106.1, AY849914.1 and AEKE03003137.1. On or before Oct 15, 2015 this sequence version replaced XM_010325685.1, NM_001247682.1. ##Evidence-Data-START## CDS exon combination :: AY849914.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA4079219, SAMEA4079221 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAGGA TACCTGAAGT TAGAGAATCA ACTAGGCAGT TTCCTATAGG GGCTAAGGTG 
GCTCATACTT TCTCAACCTC CAAGAAGGAG GTCGGTATTA GAGGCTTTCG AGATTTTGAC 
TTGGCTATTC CAATACAAAC ATGGAAAGGA AAGACCTCCT ATCAGGAGGA AGAAGACCTG 
ATGGTCGATG CTGGTACAAT CAAGCGTAGT GATGATTCAC TTGAAGATAG TGGCTCTACA 
TCATTTCATG GGGCGAGTCA TCCTCCGGAA CCTGTTGATA CAGACCTAAT GAGACCAGTG 
TATGTACCAA TTGGTCAAAA CAAAGCAGAT GGGAAATGTC TGGTGAAAAA CGTATCTTTG 
AAGGGTCCTT TCTTGGACGA TCTTTCAATC CGGATGCCAA ATGTGAAACC AAGCCCATCC 
CTTCTTTCAC CGGCAGAGAG CTTGGTTGAA GAGCCAAATG ATCTAGGTGT AATCTCCTCT 
CCATTTACAG TTCCTCGTCC TTCTCAAAAC ACAGAAACCA GTCTGCCTCC TGATTCCGAA 
GAGAAAGAAT GTATTTGGGA TGCATCACTG CCTCCAAGCG GAAATGTGAG TCCACTTAGT 
AGCATTGATA GTACTGGTGT TGTGAGATCT ATGAGTATTG TCAACAGTTG CACAAGCACG 
TACAGGAGTG ATGTGCTTAT GAGTGATGGC ATGCTTAGTG TGGACAGAAA CTATGAGAGC 
ACGAAAGGGA GCATTCGAGG GGATTCACTC GAAAGTGGTA AAACTAGCCT TAGCAGAGCA 
AGTGACAGTA GTGGACTTAG TGATGATAGT AATTGGAGTA ACATCACTGG CAGTGCCAAT 
AAGCCTCACA AAGGAAATGA TCCTAGATGG AAGGCTATTC TCGCAATTCG GGCACGTGAT 
GGTATATTAG GCATGAGCCA CTTTAAGTTA CTCAAAAGAC TTGGTTGTGG AGACATTGGA 
AGTGTTTATC TTTCTGAGCT TAGCGGGACT CGCTGCTATT TTGCAATGAA AGTGATGGAT 
AAGGCATCAC TTGCAAGCAG GAAGAAATTG ACGCGTGCTC AGACAGAAAG AGAAATCCTA 
CAGTTATTAG ACCATCCATT CTTGCCAACA TTGTACACTC ATTTTGAGAC CGATCGCTTC 
TCATGTTTGG TCATGGAATA TTGTCCTGGA GGAGATCTGC ATACTCTACG ACAGCGACAA 
CCTGGGAAGC ATTTTTCAGA ATATGCTGCA AGGTTTTATG CAGCAGAAGT TCTATTGGCG 
CTTGAATATC TACATATGCT TGGTGTAGTG TACAGGGATT TAAAACCTGA AAATGTTCTT 
GTGCGTGACG ATGGCCACAT TATGCTTTCA GATTTTGACC TCTCCTTGAG ATGTGCAGTT 
TCACCTACGC TCATAAGGAT CTCATCTGAT GATCCTTCTA AACGAGGAGC TGCATTTTGT 
GTGCAGCCAG CCTGTATTGA GCCCACAACT GTATGCATGC AGCCAGCATG CTTTCTTCCA 
CGTTTATTTC CTCAAAAGAG CAAGAAAAAA ACACCTAAGC CTCGAGCTGA TTCTGGGTTT 
CAAGCTAATT CAATGCCTGA GCTAGTTGCG GAACCTACTT CAGCACGGTC AATGTCATTT 
GTTGGGACTC ATGAATATTT GGCCCCCGAG ATTATCAAGG GAGAAGGCCA TGGCAGTGCA 
GTTGACTGGT GGACATTTGG TATTTTCCTT CATGAACTAC TCTATGGAAA GACCCCTTTC 
AAGGGATCAG GAAACAGGGC AACTCTTTTC AATGTAGTTG GCCAACAACT TAAATTTCCA 
GATTCTCCTG CAACCAGTTA TGCCAGCCGT GATCTGATAC GTGGCTTGCT TGTTAAAGAG 
CCTCAAAACC GGCTTGGGGT GAAACGAGGA GCAACTGAGA TCAAGCAACA TCCTTTCTTT 
GAAGGTGTTA ATTGGGCTCT AATACGTTGC AGTACACCAC CTGAAGTGCC AAGACCCGTG 
GAGCCAGACT ACCCTGCGAA GTACGGGCAA GTGAACCCTG TTGGGGTTGG CAATACCAGT 
AAAAGAGTGG TAGGGGCAGA TGCAAAGTCT GGGGGTAAAT ATCTTGACTT TGAGTTCTTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001234611.1
CDS	392..2494
Translation	MERIPEVRESTRQFPIGAKVAHTFSTSKKEVGIRGFRDFDLAIPIQTWKGKTSYQEEEDLMVDAGTIKRSDDSLEDSGSTSFHGASHPPEPVDTDLMRPVYVPIGQNKADGKCLVKNVSLKGPFLDDLSIRMPNVKPSPSLLSPAESLVEEPNDLGVISSPFTVPRPSQNTETSLPPDSEEKECIWDASLPPSGNVSPLSSIDSTGVVRSMSIVNSCTSTYRSDVLMSDGMLSVDRNYESTKGSIRGDSLESGKTSLSRASDSSGLSDDSNWSNITGSANKPHKGNDPRWKAILAIRARDGILGMSHFKLLKRLGCGDIGSVYLSELSGTRCYFAMKVMDKASLASRKKLTRAQTEREILQLLDHPFLPTLYTHFETDRFSCLVMEYCPGGDLHTLRQRQPGKHFSEYAARFYAAEVLLALEYLHMLGVVYRDLKPENVLVRDDGHIMLSDFDLSLRCAVSPTLIRISSDDPSKRGAAFCVQPACIEPTTVCMQPACFLPRLFPQKSKKKTPKPRADSGFQANSMPELVAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLHELLYGKTPFKGSGNRATLFNVVGQQLKFPDSPATSYASRDLIRGLLVKEPQNRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPVEPDYPAKYGQVNPVGVGNTSKRVVGADAKSGGKYLDFEFF

Target ORF information:

RefSeq Version	NM_001247682.2
Organism	Solanum lycopersicum(Lycopersicon esculentum)
Definition	Solanum lycopersicum AvrPto-dependent Pto-interacting protein 3 (Adi3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001247682.2

ORF Insert Sequence:

ATGGAAAGGA TACCTGAAGT TAGAGAATCA ACTAGGCAGT TTCCTATAGG GGCTAAGGTG 
GCTCATACTT TCTCAACCTC CAAGAAGGAG GTCGGTATTA GAGGCTTTCG AGATTTTGAC 
TTGGCTATTC CAATACAAAC ATGGAAAGGA AAGACCTCCT ATCAGGAGGA AGAAGACCTG 
ATGGTCGATG CTGGTACAAT CAAGCGTAGT GATGATTCAC TTGAAGATAG TGGCTCTACA 
TCATTTCATG GGGCGAGTCA TCCTCCGGAA CCTGTTGATA CAGACCTAAT GAGACCAGTG 
TATGTACCAA TTGGTCAAAA CAAAGCAGAT GGGAAATGTC TGGTGAAAAA CGTATCTTTG 
AAGGGTCCTT TCTTGGACGA TCTTTCAATC CGGATGCCAA ATGTGAAACC AAGCCCATCC 
CTTCTTTCAC CGGCAGAGAG CTTGGTTGAA GAGCCAAATG ATCTAGGTGT AATCTCCTCT 
CCATTTACAG TTCCTCGTCC TTCTCAAAAC ACAGAAACCA GTCTGCCTCC TGATTCCGAA 
GAGAAAGAAT GTATTTGGGA TGCATCACTG CCTCCAAGCG GAAATGTGAG TCCACTTAGT 
AGCATTGATA GTACTGGTGT TGTGAGATCT ATGAGTATTG TCAACAGTTG CACAAGCACG 
TACAGGAGTG ATGTGCTTAT GAGTGATGGC ATGCTTAGTG TGGACAGAAA CTATGAGAGC 
ACGAAAGGGA GCATTCGAGG GGATTCACTC GAAAGTGGTA AAACTAGCCT TAGCAGAGCA 
AGTGACAGTA GTGGACTTAG TGATGATAGT AATTGGAGTA ACATCACTGG CAGTGCCAAT 
AAGCCTCACA AAGGAAATGA TCCTAGATGG AAGGCTATTC TCGCAATTCG GGCACGTGAT 
GGTATATTAG GCATGAGCCA CTTTAAGTTA CTCAAAAGAC TTGGTTGTGG AGACATTGGA 
AGTGTTTATC TTTCTGAGCT TAGCGGGACT CGCTGCTATT TTGCAATGAA AGTGATGGAT 
AAGGCATCAC TTGCAAGCAG GAAGAAATTG ACGCGTGCTC AGACAGAAAG AGAAATCCTA 
CAGTTATTAG ACCATCCATT CTTGCCAACA TTGTACACTC ATTTTGAGAC CGATCGCTTC 
TCATGTTTGG TCATGGAATA TTGTCCTGGA GGAGATCTGC ATACTCTACG ACAGCGACAA 
CCTGGGAAGC ATTTTTCAGA ATATGCTGCA AGGTTTTATG CAGCAGAAGT TCTATTGGCG 
CTTGAATATC TACATATGCT TGGTGTAGTG TACAGGGATT TAAAACCTGA AAATGTTCTT 
GTGCGTGACG ATGGCCACAT TATGCTTTCA GATTTTGACC TCTCCTTGAG ATGTGCAGTT 
TCACCTACGC TCATAAGGAT CTCATCTGAT GATCCTTCTA AACGAGGAGC TGCATTTTGT 
GTGCAGCCAG CCTGTATTGA GCCCACAACT GTATGCATGC AGCCAGCATG CTTTCTTCCA 
CGTTTATTTC CTCAAAAGAG CAAGAAAAAA ACACCTAAGC CTCGAGCTGA TTCTGGGTTT 
CAAGCTAATT CAATGCCTGA GCTAGTTGCG GAACCTACTT CAGCACGGTC AATGTCATTT 
GTTGGGACTC ATGAATATTT GGCCCCCGAG ATTATCAAGG GAGAAGGCCA TGGCAGTGCA 
GTTGACTGGT GGACATTTGG TATTTTCCTT CATGAACTAC TCTATGGAAA GACCCCTTTC 
AAGGGATCAG GAAACAGGGC AACTCTTTTC AATGTAGTTG GCCAACAACT TAAATTTCCA 
GATTCTCCTG CAACCAGTTA TGCCAGCCGT GATCTGATAC GTGGCTTGCT TGTTAAAGAG 
CCTCAAAACC GGCTTGGGGT GAAACGAGGA GCAACTGAGA TCAAGCAACA TCCTTTCTTT 
GAAGGTGTTA ATTGGGCTCT AATACGTTGC AGTACACCAC CTGAAGTGCC AAGACCCGTG 
GAGCCAGACT ACCCTGCGAA GTACGGGCAA GTGAACCCTG TTGGGGTTGG CAATACCAGT 
AAAAGAGTGG TAGGGGCAGA TGCAAAGTCT GGGGGTAAAT ATCTTGACTT TGAGTTCTTT 
TAG