ddx1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx1
Entrez Gene ID	111238354
Full Name	DEAD-box helicase 1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022767815.1	XP_022623536.1	ATP-dependent RNA helicase DDX1

Pathways from BioSystems
Gene Ontology

The following ddx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe208890	XM_022767815.1 Latest version!	Seriola dumerili DEAD-box helicase 1 (ddx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx1 ( XM_022767815.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022623536.1 Seriola dumerili DEAD-box helicase 1 (ddx1), mRNA.

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CloneID	OSe208890
Clone ID Related Accession (Same CDS sequence)	XM_022767815.1
Accession Version	XM_022767815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	ATP-dependent RNA helicase DDX1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174280.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGT ATTCTGAAAT GGGGGTTATG CCTGAAATTG CGCAGGCTGT GGATGAGATG 
GAATGGCTGC TACCTACTGA CATCCAGGCT GAGTCCATTC CCCTAATTCT TGGTGGAGGA 
GATGTACTTA TGGCAGCAGA AACTGGTAGT GGCAAAACAG GAGCCTTCAG TATCCCTGTG 
ATCCAGATTG TGTACGAGAC CCTGAAGGAT CACCAGGAGG GCAAGAAGGG CAAAGCCTCC 
ATCAAGACTG GAGGAGCCAT TTTCAACACT TGGCAGATGA ATCCTTACGA TCGCAGTACA 
GCTTTTGCAA TTGGTCCAGA TGGACTGTGC TGCCAGAGCA GGGAGTTTAA AGAGTGGCAT 
GGCTGTCGCT CTACAAAAGG TGTCACCAAA GGGAAGTACT GTTATGAGGT GACCTGCCAT 
GATCAGGGTC TGTGTCGAGT AGGATGGTCC ACCATTCAGG CAGCACTGGA CTTGGGAACT 
GATAAGCATG GTTTTGGTTT TGGAGGGACT GGAAAAAAAT CCAACAACAA GCAGTTTGAC 
AGCTATGGAG AGGAGTTTAC CATGCATGAC ACTATTGGAT GCTACCTGGA TTTAGACAAT 
GGCCAAATTT CCTTCTCCAA AAATGGTAAT GACCTTGGTT TGGCTTTTGA GATCCCTCAA 
AATCTGAGGA ATCAGCCTTT CTTTGCCTCC TGTGTTCTAA AGAATGCAGA GCTGAAGTTC 
AACTTTGGTG GAGAAAACTT TAAACACCCC CCAAAGAGTG GATTTGTTGC CCTGGATCAG 
GCATCAGAGG GTCACACAGC CAAATCTTCT CAGACAGGCA GTGCCAAAGT CAGTCAGGTG 
AAAGCAGCAT CCAATGCACC CAAAGCCCTG ATCATTGAGC CATCTAAAGA ATTAGCTGAG 
CAAACTCTCA ACAATGTCAA ACAATTCAAG AAATATGTGG ACAACCCCAA ACTCAGGGAA 
CTACTTGTGA TTGGTGGTGT GGCTGCCAAG GATCAGTTGG CTGCTCTGGA ACAGGGGGTT 
GACATTGTGG TGGGAACACC TGGCAGACTG GATGACCTCA TATCCACTGG GAAACTCAGT 
CTGTCCCAGA TCCGTTTTCT GGTCCTGGAT GAGTGTGATG GGCTGCTGTC AGCAGGCTAT 
ACAGATTTTA TCAACAGAAT CTATAACCAA ATCCCCCAGG TCACCTCTGA TGGCAAGAGA 
CTGCAGGTGA TTGTATGCTC AGCCACTCTG CATTCCTTTG ACGTGAAGAA GTTGTCTGAG 
CGTATCATGC ACTTTCCCAC ATGGGTGGAC CTGAAGGGGG AGGACTCTGT TCCAGAGACT 
GTCCACCATG TAGTGGTGCC TGTTAATCCT AAGAGTGATC GTCTCTGGGA GCGTCTGGGC 
AAGAACCACA TCCGGACTGA TGAAGTCCAT GCCAAAGATA ACACCAGACC AGGAGCAAAT 
TCTGCAGAAA TGTGGTCAGA AGCCATCAAG GTGCTAAAGG GTGAGTACAC AGTGAAGGCC 
ATCAAAGAAC ACAAGATGGA CCAGGCCATT ATCTTCTGTC GCACCAAGAT CGACTGTGAC 
AACATGGAGC AGTACTTCAT CCAGCAAGGA GGAGGTCCAG ACAGTAAAAA CCACCAGTTT 
TCCTGTGTTT GTCTCCATGG TGATCGTAAG CCCAATGAGC GGAAAAATAA CTTAGAGCGT 
TTCAAGAAAA AAGAGGTGAG GCTCTTGATC TGCACAGATG TAGCCGCCAG AGGAATTGAC 
ATCCATGGAG TTCCATACGT GATTAACGTC ACCCTGCCAG ATGAGAAGCA GAACTACGTC 
CATCGTATCG GCAGAGTCGG AAGAGCAGAG AGAATGGGAC TGGCTATCTC CTTAGTTGCA 
ATGGAAAAAG AGAAGGTGTG GTACCATGTT TGCCCGAACC GAGGCAGAGG TTGCTACAAC 
ACCCGACTGA AGGAGGATGG AGGTTGCACC ATCTGGTACA ATGAGAAAGA GCTCCTGTCA 
GAAGTTGAGG AGCACCTAAA GTGCACCATC ACCCAGTGTG AGCCAGACAT CAAAGTACCT 
CTAGATGAGT TTGATGGAAA GGTCACCTAT GGTCAGCGCA GGGCATTGGG AGGTGGTAAC 
TACAAGGGTC ATGTGGATGT ACTAGCTCCA ACAGTCCAGG AGCTGGCTAA CCTTGAGCAG 
GAAGCCCAAA CATCCTTCCT CCACATGGGA TACATGCCAA ACCAACTTTT CAGAGCCTTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022623536.1
CDS	23..2245
Translation	MAAYSEMGVMPEIAQAVDEMEWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDHQEGKKGKASIKTGGAIFNTWQMNPYDRSTAFAIGPDGLCCQSREFKEWHGCRSTKGVTKGKYCYEVTCHDQGLCRVGWSTIQAALDLGTDKHGFGFGGTGKKSNNKQFDSYGEEFTMHDTIGCYLDLDNGQISFSKNGNDLGLAFEIPQNLRNQPFFASCVLKNAELKFNFGGENFKHPPKSGFVALDQASEGHTAKSSQTGSAKVSQVKAASNAPKALIIEPSKELAEQTLNNVKQFKKYVDNPKLRELLVIGGVAAKDQLAALEQGVDIVVGTPGRLDDLISTGKLSLSQIRFLVLDECDGLLSAGYTDFINRIYNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSERIMHFPTWVDLKGEDSVPETVHHVVVPVNPKSDRLWERLGKNHIRTDEVHAKDNTRPGANSAEMWSEAIKVLKGEYTVKAIKEHKMDQAIIFCRTKIDCDNMEQYFIQQGGGPDSKNHQFSCVCLHGDRKPNERKNNLERFKKKEVRLLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVAMEKEKVWYHVCPNRGRGCYNTRLKEDGGCTIWYNEKELLSEVEEHLKCTITQCEPDIKVPLDEFDGKVTYGQRRALGGGNYKGHVDVLAPTVQELANLEQEAQTSFLHMGYMPNQLFRAF

Target ORF information:

RefSeq Version	XM_022767815.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili DEAD-box helicase 1 (ddx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022767815.1

ORF Insert Sequence:

ATGGCAGCGT ATTCTGAAAT GGGGGTTATG CCTGAAATTG CGCAGGCTGT GGATGAGATG 
GAATGGCTGC TACCTACTGA CATCCAGGCT GAGTCCATTC CCCTAATTCT TGGTGGAGGA 
GATGTACTTA TGGCAGCAGA AACTGGTAGT GGCAAAACAG GAGCCTTCAG TATCCCTGTG 
ATCCAGATTG TGTACGAGAC CCTGAAGGAT CACCAGGAGG GCAAGAAGGG CAAAGCCTCC 
ATCAAGACTG GAGGAGCCAT TTTCAACACT TGGCAGATGA ATCCTTACGA TCGCAGTACA 
GCTTTTGCAA TTGGTCCAGA TGGACTGTGC TGCCAGAGCA GGGAGTTTAA AGAGTGGCAT 
GGCTGTCGCT CTACAAAAGG TGTCACCAAA GGGAAGTACT GTTATGAGGT GACCTGCCAT 
GATCAGGGTC TGTGTCGAGT AGGATGGTCC ACCATTCAGG CAGCACTGGA CTTGGGAACT 
GATAAGCATG GTTTTGGTTT TGGAGGGACT GGAAAAAAAT CCAACAACAA GCAGTTTGAC 
AGCTATGGAG AGGAGTTTAC CATGCATGAC ACTATTGGAT GCTACCTGGA TTTAGACAAT 
GGCCAAATTT CCTTCTCCAA AAATGGTAAT GACCTTGGTT TGGCTTTTGA GATCCCTCAA 
AATCTGAGGA ATCAGCCTTT CTTTGCCTCC TGTGTTCTAA AGAATGCAGA GCTGAAGTTC 
AACTTTGGTG GAGAAAACTT TAAACACCCC CCAAAGAGTG GATTTGTTGC CCTGGATCAG 
GCATCAGAGG GTCACACAGC CAAATCTTCT CAGACAGGCA GTGCCAAAGT CAGTCAGGTG 
AAAGCAGCAT CCAATGCACC CAAAGCCCTG ATCATTGAGC CATCTAAAGA ATTAGCTGAG 
CAAACTCTCA ACAATGTCAA ACAATTCAAG AAATATGTGG ACAACCCCAA ACTCAGGGAA 
CTACTTGTGA TTGGTGGTGT GGCTGCCAAG GATCAGTTGG CTGCTCTGGA ACAGGGGGTT 
GACATTGTGG TGGGAACACC TGGCAGACTG GATGACCTCA TATCCACTGG GAAACTCAGT 
CTGTCCCAGA TCCGTTTTCT GGTCCTGGAT GAGTGTGATG GGCTGCTGTC AGCAGGCTAT 
ACAGATTTTA TCAACAGAAT CTATAACCAA ATCCCCCAGG TCACCTCTGA TGGCAAGAGA 
CTGCAGGTGA TTGTATGCTC AGCCACTCTG CATTCCTTTG ACGTGAAGAA GTTGTCTGAG 
CGTATCATGC ACTTTCCCAC ATGGGTGGAC CTGAAGGGGG AGGACTCTGT TCCAGAGACT 
GTCCACCATG TAGTGGTGCC TGTTAATCCT AAGAGTGATC GTCTCTGGGA GCGTCTGGGC 
AAGAACCACA TCCGGACTGA TGAAGTCCAT GCCAAAGATA ACACCAGACC AGGAGCAAAT 
TCTGCAGAAA TGTGGTCAGA AGCCATCAAG GTGCTAAAGG GTGAGTACAC AGTGAAGGCC 
ATCAAAGAAC ACAAGATGGA CCAGGCCATT ATCTTCTGTC GCACCAAGAT CGACTGTGAC 
AACATGGAGC AGTACTTCAT CCAGCAAGGA GGAGGTCCAG ACAGTAAAAA CCACCAGTTT 
TCCTGTGTTT GTCTCCATGG TGATCGTAAG CCCAATGAGC GGAAAAATAA CTTAGAGCGT 
TTCAAGAAAA AAGAGGTGAG GCTCTTGATC TGCACAGATG TAGCCGCCAG AGGAATTGAC 
ATCCATGGAG TTCCATACGT GATTAACGTC ACCCTGCCAG ATGAGAAGCA GAACTACGTC 
CATCGTATCG GCAGAGTCGG AAGAGCAGAG AGAATGGGAC TGGCTATCTC CTTAGTTGCA 
ATGGAAAAAG AGAAGGTGTG GTACCATGTT TGCCCGAACC GAGGCAGAGG TTGCTACAAC 
ACCCGACTGA AGGAGGATGG AGGTTGCACC ATCTGGTACA ATGAGAAAGA GCTCCTGTCA 
GAAGTTGAGG AGCACCTAAA GTGCACCATC ACCCAGTGTG AGCCAGACAT CAAAGTACCT 
CTAGATGAGT TTGATGGAAA GGTCACCTAT GGTCAGCGCA GGGCATTGGG AGGTGGTAAC 
TACAAGGGTC ATGTGGATGT ACTAGCTCCA ACAGTCCAGG AGCTGGCTAA CCTTGAGCAG 
GAAGCCCAAA CATCCTTCCT CCACATGGGA TACATGCCAA ACCAACTTTT CAGAGCCTTC 
TAA