LOC111224075 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111224075
Entrez Gene ID	111224075
Full Name	cullin-3
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022748459.1	XP_022604180.1	cullin-3

Pathways from BioSystems
Gene Ontology

The following LOC111224075 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111224075 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe217991	XM_022748459.1 Latest version!	Seriola dumerili cullin-3 (LOC111224075), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111224075 ( XM_022748459.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022604180.1 Seriola dumerili cullin-3 (LOC111224075), mRNA.

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CloneID	OSe217991
Clone ID Related Accession (Same CDS sequence)	XM_022748459.1
Accession Version	XM_022748459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	cullin-3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174377.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAATC TCAGCAAAGG CGGCACCAAG AAGGACACCA AAATGAGGAT ACGGGCCTTT 
CCTATGACGA TGGATGAGAA GTACGTCAAC AATATTTGGG ACCTTCTGAA GAATGCCATC 
CAGGAGATAC AGAGGAAGAA TAACAGTGGT CTGAGCTTTG AGGAACTATA CAGGAACGCC 
TACACGATGG TGCTCCACAA ACATGGAGAG AAGCTGTACA CAGGCCTGCG GGAGGTCGTC 
ACTGAACACC TTATCAACAA AGTACGAGAA GATGTCCTCA ACTCCCTAAA TAATAACTTC 
CTCCAAACCC TAAATCAGGC CTGGAATGAC CATCAGACGG CGATGGTCAT GATCAGAGAC 
ATCCTGATGT ATATGGACCG AGTTTATGTA CAGCAGAACA ATGTAGAGAA TGTCTACAAC 
CTGGGTCTTA TCATCTTTAG GGATCAGGTG GTTCGCTATG GCTGCATCAG AGACCACCTC 
CGACAGACCC TGCTGGACAT GATCGCACGC GAGAGGAAGG GGGAAGTGGT GGACAGGGGG 
GCCATTAGAA ATGCCTGCCA GATGTTAATG ATCCTCGGCC TTGAAGGGAG ATCTGTTTAT 
GAAGAAGACT TTGAGGCACC GTTCTTAGAA ATGTCTGCAG AATTTTTCCA GATGGAGAGC 
CAAAAGTTCC TTGCAGAAAA CAGTGCCAGC GTGTACATAA AGAAGGTGGA AGCCAGGATT 
AATGAGGAGA TTGAGCGAGT GATGCACTGC CTGGATAAGT CCACAGAGGA GCCTATTGTC 
AAGGTGGTGG AAAGGGAACT CATCTCCAAA CACATGAAAA CCATTGTAGA GATGGAGAAC 
TCAGGCCTGG TCCACATGCT CAAGAACGGC AAGACAGACG ACTTGGCGTG CATGTACAAG 
CTGTTCAGCA GGGTCCCCAA TGGGCTGAAG ACCATGTGTG AGTGTATGAG CTCATACCTG 
CGGGAGCAAG GCAAGGCTCT CGTGTCAGAG GAGGGCGAGG GAAAGAATCC TGTAGACTAC 
ATCCAGGGTT TGCTGGACCT GAAGTCCCGT TTCGACCGCT TCCTCCAGGA GTCTTTCAAT 
AATGACAGGC TCTTCAAACA AACCATTGCT GGTGACTTTG AGTACTTCCT TAACCTCAAC 
TCTCGCTCGC CCGAGTACCT CTCACTTTTC ATCGATGACA AATTGAAGAA AGGTGTTAAA 
GGGCTGACAG AGCAGGAGGT GGAGTCTATA CTGGACAAGG CCATGGTGCT TTTCCGCTTC 
ATGCAGGAAA AAGACGTGTT TGAGAGGTAC TACAAGCAGC ACCTGGCCCG CAGGCTGCTC 
ACCAACAAGA GCGTCTCTGA TGACTCTGAG AAAAACATGA TCTCCAAGCT TAAGACTGAA 
TGCGGCTGTC AGTTCACATC CAAACTGGAG GGCATGTTCC GGGATATGAG CATCTCGAAC 
ACCACCATGG ATGAGTTTAG ACAACATCTA CAGACAACTG GGGTGTCACT TGGAGGGGTT 
GATCTCACTG TGAGAGTCTT GACCACGGGA TACTGGCCAA CACAATCAGC AACACCCAAG 
TGCAATATCC CTCCTTCACC ACGACATGCA TTTGAAGTAT TTAGGAGGTT TTATCTTGGT 
AAGCACAGCG GCAGACAACT CACACTGCAG CACCATATGG GCTCTGCAGA TCTAAATGCC 
ACCTTCTATG GTCCCATCAA AAAGGAGGAC GGGTCAGAGG TTGGAGTGGG AGGAGCCCAG 
GTGACAGGCT CCAACACCAG GAAGCACATC CTGCAGGTCT CCACCTTCCA GATGACCATC 
CTCATGCTTT TCAACAACAG AGAGAAGTCC ACCTTTGAGG AGATCCAGCA GGAGACAGAC 
ATCCCAGAGA GGGAGCTGGT GCGAGCGCTG CAGTCGCTGG CCTGTGGGAA ACCCACTCAG 
AGAGTCCTCA CTAAGGAGCC CAAGTCCAAG GAGATTGAGA ATGGCCATGT GTTTACAGTC 
AATGACCAGT TTACCTCCAA ACTACACCGT GTCAAGATCC AGACAGTGGC TGCTAAACAG 
GGAGAGTCAG ACCCAGAGAG GAAGGAGACA CGACAGAAAG TGGACGACGA CAGGAAGCAT 
GAGATCGAAG CTGCCATCGT TCGCATCATG AAGTCTAGAA AGAAGATGCA GCACAATGTC 
CTAGTAGCAG AGGTCACACA GCAGTTGCGA GCTCGATTCC TCCCTAGTCC TGTAGTCATC 
AAGAAGCGCA TTGAAGGACT CATTGAGAGG GAATATTTGG CACGAACACC GGAGGACCGC 
AAAGTGTACA CTTATGTAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022604180.1
CDS	197..2500
Translation	MSNLSKGGTKKDTKMRIRAFPMTMDEKYVNNIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTDDLACMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLQESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVESILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQTTGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPSPRHAFEVFRRFYLGKHSGRQLTLQHHMGSADLNATFYGPIKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKSTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHVFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLRARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA

Target ORF information:

RefSeq Version	XM_022748459.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili cullin-3 (LOC111224075), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022748459.1

ORF Insert Sequence:

ATGTCCAATC TCAGCAAAGG CGGCACCAAG AAGGACACCA AAATGAGGAT ACGGGCCTTT 
CCTATGACGA TGGATGAGAA GTACGTCAAC AATATTTGGG ACCTTCTGAA GAATGCCATC 
CAGGAGATAC AGAGGAAGAA TAACAGTGGT CTGAGCTTTG AGGAACTATA CAGGAACGCC 
TACACGATGG TGCTCCACAA ACATGGAGAG AAGCTGTACA CAGGCCTGCG GGAGGTCGTC 
ACTGAACACC TTATCAACAA AGTACGAGAA GATGTCCTCA ACTCCCTAAA TAATAACTTC 
CTCCAAACCC TAAATCAGGC CTGGAATGAC CATCAGACGG CGATGGTCAT GATCAGAGAC 
ATCCTGATGT ATATGGACCG AGTTTATGTA CAGCAGAACA ATGTAGAGAA TGTCTACAAC 
CTGGGTCTTA TCATCTTTAG GGATCAGGTG GTTCGCTATG GCTGCATCAG AGACCACCTC 
CGACAGACCC TGCTGGACAT GATCGCACGC GAGAGGAAGG GGGAAGTGGT GGACAGGGGG 
GCCATTAGAA ATGCCTGCCA GATGTTAATG ATCCTCGGCC TTGAAGGGAG ATCTGTTTAT 
GAAGAAGACT TTGAGGCACC GTTCTTAGAA ATGTCTGCAG AATTTTTCCA GATGGAGAGC 
CAAAAGTTCC TTGCAGAAAA CAGTGCCAGC GTGTACATAA AGAAGGTGGA AGCCAGGATT 
AATGAGGAGA TTGAGCGAGT GATGCACTGC CTGGATAAGT CCACAGAGGA GCCTATTGTC 
AAGGTGGTGG AAAGGGAACT CATCTCCAAA CACATGAAAA CCATTGTAGA GATGGAGAAC 
TCAGGCCTGG TCCACATGCT CAAGAACGGC AAGACAGACG ACTTGGCGTG CATGTACAAG 
CTGTTCAGCA GGGTCCCCAA TGGGCTGAAG ACCATGTGTG AGTGTATGAG CTCATACCTG 
CGGGAGCAAG GCAAGGCTCT CGTGTCAGAG GAGGGCGAGG GAAAGAATCC TGTAGACTAC 
ATCCAGGGTT TGCTGGACCT GAAGTCCCGT TTCGACCGCT TCCTCCAGGA GTCTTTCAAT 
AATGACAGGC TCTTCAAACA AACCATTGCT GGTGACTTTG AGTACTTCCT TAACCTCAAC 
TCTCGCTCGC CCGAGTACCT CTCACTTTTC ATCGATGACA AATTGAAGAA AGGTGTTAAA 
GGGCTGACAG AGCAGGAGGT GGAGTCTATA CTGGACAAGG CCATGGTGCT TTTCCGCTTC 
ATGCAGGAAA AAGACGTGTT TGAGAGGTAC TACAAGCAGC ACCTGGCCCG CAGGCTGCTC 
ACCAACAAGA GCGTCTCTGA TGACTCTGAG AAAAACATGA TCTCCAAGCT TAAGACTGAA 
TGCGGCTGTC AGTTCACATC CAAACTGGAG GGCATGTTCC GGGATATGAG CATCTCGAAC 
ACCACCATGG ATGAGTTTAG ACAACATCTA CAGACAACTG GGGTGTCACT TGGAGGGGTT 
GATCTCACTG TGAGAGTCTT GACCACGGGA TACTGGCCAA CACAATCAGC AACACCCAAG 
TGCAATATCC CTCCTTCACC ACGACATGCA TTTGAAGTAT TTAGGAGGTT TTATCTTGGT 
AAGCACAGCG GCAGACAACT CACACTGCAG CACCATATGG GCTCTGCAGA TCTAAATGCC 
ACCTTCTATG GTCCCATCAA AAAGGAGGAC GGGTCAGAGG TTGGAGTGGG AGGAGCCCAG 
GTGACAGGCT CCAACACCAG GAAGCACATC CTGCAGGTCT CCACCTTCCA GATGACCATC 
CTCATGCTTT TCAACAACAG AGAGAAGTCC ACCTTTGAGG AGATCCAGCA GGAGACAGAC 
ATCCCAGAGA GGGAGCTGGT GCGAGCGCTG CAGTCGCTGG CCTGTGGGAA ACCCACTCAG 
AGAGTCCTCA CTAAGGAGCC CAAGTCCAAG GAGATTGAGA ATGGCCATGT GTTTACAGTC 
AATGACCAGT TTACCTCCAA ACTACACCGT GTCAAGATCC AGACAGTGGC TGCTAAACAG 
GGAGAGTCAG ACCCAGAGAG GAAGGAGACA CGACAGAAAG TGGACGACGA CAGGAAGCAT 
GAGATCGAAG CTGCCATCGT TCGCATCATG AAGTCTAGAA AGAAGATGCA GCACAATGTC 
CTAGTAGCAG AGGTCACACA GCAGTTGCGA GCTCGATTCC TCCCTAGTCC TGTAGTCATC 
AAGAAGCGCA TTGAAGGACT CATTGAGAGG GAATATTTGG CACGAACACC GGAGGACCGC 
AAAGTGTACA CTTATGTAGC ATAA