mcc cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	mcc
Entrez Gene ID	111216840
Full Name	mutated in colorectal cancers
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022738467.1	XP_022594188.1	colorectal mutant cancer protein

Pathways from BioSystems
Gene Ontology

The following mcc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe206449	XM_022738467.1 Latest version!	Seriola dumerili mutated in colorectal cancers (mcc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcc ( XM_022738467.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022594188.1 Seriola dumerili mutated in colorectal cancers (mcc), mRNA.

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CloneID	OSe206449
Clone ID Related Accession (Same CDS sequence)	XM_022738467.1
Accession Version	XM_022738467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	colorectal mutant cancer protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174257.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATT CCAGCCTGGT CAGATGTGAC AGCCTGGATA CTGGGTCAGG GTGTGACAGG 
GAGGTGATTG ACCATATGAC CTTTGACCCT GAGGGCTCCG ACTCCACAGC AAACCTCGAG 
CCTTCACCAG CAGAATTGGC TCAGTGTGAG GCGGAGGTGG GCACACTACT CAGCATCATC 
GCTGAACTGA ACAAGAAGAT GGGGTCATTA AAGGCACCGA GTGAACCAGG GGACCTGAGA 
CCACCAGGAC CATGCAGGCC TTTGGTTCCT GACCTTTTGT CTCACAGACT GGTCAGAAGC 
AGCCCAGAGA GACATGTTGT CTCTGCTGCC ACATCTAAAC CTGCACTGAT TGACCGAGGG 
GGCAGTGGTG TAGTGTGGAC AAAGCTGCAG GAGGTTTTAT CATCAGTGGA GGACTCCATC 
AGCTGTAGAA GGACCTGGGC CGCTCCCATC ACAGCTTCTG ACCAGGACAA GCACGAAGAG 
CATCTGAGAG CAGCGCAGGA GAGCTGGGTT AAAGCAGCTG AGATGTTGGA GGAGATGGAA 
AAAGAGTTTG GGATATCGTG CCCATCAGCC CTGCCAAAGG ACCAGTATCA GAGGGACACC 
GTGGATCTGG AGAAACATGA CTCTGCTCTC AGAAGCACCT CTCACAGTCA CCAGGAGGAG 
CTGGAGAGAG CACAAAACAC AATCAGCCAG ATGGAGGAGG AGAAGAACAA GCTGGCTGGT 
CTCCATAAGG CCTGGAGGTC AGGCAGTCGC TCTCCTTCCT ACCGTCCCAC TGCTGGGCCT 
CTTAGTCCAG ACTTGGCCTC TCCTCCCTTT CCTGGTTCTC CTTTACTCCA CAGAAGAACA 
GCCAGAGGTC CGACACCTCT GTCTGCTGGG GGAGGCGGCT CTCCACTGGG CTCTGTTAAC 
TCTGGCAGTC CTTGTCCCAG CCCCATCAGC CTGGAGTCCG AGACAGAACG ACTGAACAGG 
TACATCGAGA GGCTGAAGGC TAGAAATGAA CGTCTGACTG CAGCTCTGGA GCGAAGGAAG 
GGAGAGTCGG AGCAGATCAC TATCACACTA AACAGACTTG AGTCCGACTG CTCTGCCCTG 
CAGTTGGCTC TCAAATACTG TGAGGAGTGT GAGGAGGCCT ACAGCGAGCT ACTGTCACTT 
TATGAGAGCA AGAGGCAGCA AAGCATTCCT CTGCAGACAG ACCCAGCAGA GGCTGTCGGT 
GACAGGCAGC AGCCCGACAG TCCATCATCT CAGCTCAGGA ACATGGGAAC TGAAGAGCTG 
TCCACCTCCT TCTCAACAGC AGGGGTCACA GAGGAGACGG AAACACGGAG TTACACAGGG 
CAGAGGACAC CTGAGCTGGT GAATCGAGAG GCTGCTCTCC GTCAACAAAT TGAGCGTCTG 
AAGAGGGACC GGGCAGCCAT TTGCCTCCCG AAACCAAGCC CAGGAGTGGA GAGCAAACTG 
AGCCCTGAAA CCGGTCACCC AGCTGGGTCA CGAGGGGGAC ATGTGGTTAA AGATAACGTC 
AAACCTCCTG ATGTGAAGAA AGAGAAGGCT TCCCTCTTCT ACGAACTCAT TTCTGTCAGG 
GAGGAGATGT CAGATCTGCG AGCTCTGATC CGCCTGAAAG AGAAAGACCT GAGGTGTCTG 
GAGTGGAGTT TAATGGCCCA GAAGGCCCAG GAAGCAGCTG GAGTTTTCAT CCCTGAAAGT 
CTCAGAGAGG AGCTGGAGGA GGATCGTAAG ACTGAACAAC AGAGGCTCTG TGAGACCACA 
GCTAAACTGG GTGGTGATGG GGACATTGCC GGACCTCGAA CCAGGCCCAT TCTGAAAGAG 
CTCCAGGCCG TCCTGCAAAG GGAACAAGCC CTGAAGAAGA GATTAGCTTT AGTCCATGAC 
TCATTGAGCA CAGCTCTCTC AGACAGCGCC TCGCACAGGA GGGACAATGG AGAGCAGATT 
GCACGGCTCA CACAAGCTCA CAGTAAAGCC TTGAGCTCGT ATCGGCAGAT TCGCAGGAAG 
TATCGGGAGC AGGTATGGCG GCTGGAGCAG AAAGTGGTGG CCATGACGGA GAGTCACCAC 
AACCAGAGTG GAGCACCAAA AACGGCAGAG GAGGCCGTGG AGTGGAGGAG GGAAGAGACT 
ATTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022594188.1
CDS	174..2282
Translation	MEDSSLVRCDSLDTGSGCDREVIDHMTFDPEGSDSTANLEPSPAELAQCEAEVGTLLSIIAELNKKMGSLKAPSEPGDLRPPGPCRPLVPDLLSHRLVRSSPERHVVSAATSKPALIDRGGSGVVWTKLQEVLSSVEDSISCRRTWAAPITASDQDKHEEHLRAAQESWVKAAEMLEEMEKEFGISCPSALPKDQYQRDTVDLEKHDSALRSTSHSHQEELERAQNTISQMEEEKNKLAGLHKAWRSGSRSPSYRPTAGPLSPDLASPPFPGSPLLHRRTARGPTPLSAGGGGSPLGSVNSGSPCPSPISLESETERLNRYIERLKARNERLTAALERRKGESEQITITLNRLESDCSALQLALKYCEECEEAYSELLSLYESKRQQSIPLQTDPAEAVGDRQQPDSPSSQLRNMGTEELSTSFSTAGVTEETETRSYTGQRTPELVNREAALRQQIERLKRDRAAICLPKPSPGVESKLSPETGHPAGSRGGHVVKDNVKPPDVKKEKASLFYELISVREEMSDLRALIRLKEKDLRCLEWSLMAQKAQEAAGVFIPESLREELEEDRKTEQQRLCETTAKLGGDGDIAGPRTRPILKELQAVLQREQALKKRLALVHDSLSTALSDSASHRRDNGEQIARLTQAHSKALSSYRQIRRKYREQVWRLEQKVVAMTESHHNQSGAPKTAEEAVEWRREETIL

Target ORF information:

RefSeq Version	XM_022738467.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili mutated in colorectal cancers (mcc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022738467.1

ORF Insert Sequence:

ATGGAGGATT CCAGCCTGGT CAGATGTGAC AGCCTGGATA CTGGGTCAGG GTGTGACAGG 
GAGGTGATTG ACCATATGAC CTTTGACCCT GAGGGCTCCG ACTCCACAGC AAACCTCGAG 
CCTTCACCAG CAGAATTGGC TCAGTGTGAG GCGGAGGTGG GCACACTACT CAGCATCATC 
GCTGAACTGA ACAAGAAGAT GGGGTCATTA AAGGCACCGA GTGAACCAGG GGACCTGAGA 
CCACCAGGAC CATGCAGGCC TTTGGTTCCT GACCTTTTGT CTCACAGACT GGTCAGAAGC 
AGCCCAGAGA GACATGTTGT CTCTGCTGCC ACATCTAAAC CTGCACTGAT TGACCGAGGG 
GGCAGTGGTG TAGTGTGGAC AAAGCTGCAG GAGGTTTTAT CATCAGTGGA GGACTCCATC 
AGCTGTAGAA GGACCTGGGC CGCTCCCATC ACAGCTTCTG ACCAGGACAA GCACGAAGAG 
CATCTGAGAG CAGCGCAGGA GAGCTGGGTT AAAGCAGCTG AGATGTTGGA GGAGATGGAA 
AAAGAGTTTG GGATATCGTG CCCATCAGCC CTGCCAAAGG ACCAGTATCA GAGGGACACC 
GTGGATCTGG AGAAACATGA CTCTGCTCTC AGAAGCACCT CTCACAGTCA CCAGGAGGAG 
CTGGAGAGAG CACAAAACAC AATCAGCCAG ATGGAGGAGG AGAAGAACAA GCTGGCTGGT 
CTCCATAAGG CCTGGAGGTC AGGCAGTCGC TCTCCTTCCT ACCGTCCCAC TGCTGGGCCT 
CTTAGTCCAG ACTTGGCCTC TCCTCCCTTT CCTGGTTCTC CTTTACTCCA CAGAAGAACA 
GCCAGAGGTC CGACACCTCT GTCTGCTGGG GGAGGCGGCT CTCCACTGGG CTCTGTTAAC 
TCTGGCAGTC CTTGTCCCAG CCCCATCAGC CTGGAGTCCG AGACAGAACG ACTGAACAGG 
TACATCGAGA GGCTGAAGGC TAGAAATGAA CGTCTGACTG CAGCTCTGGA GCGAAGGAAG 
GGAGAGTCGG AGCAGATCAC TATCACACTA AACAGACTTG AGTCCGACTG CTCTGCCCTG 
CAGTTGGCTC TCAAATACTG TGAGGAGTGT GAGGAGGCCT ACAGCGAGCT ACTGTCACTT 
TATGAGAGCA AGAGGCAGCA AAGCATTCCT CTGCAGACAG ACCCAGCAGA GGCTGTCGGT 
GACAGGCAGC AGCCCGACAG TCCATCATCT CAGCTCAGGA ACATGGGAAC TGAAGAGCTG 
TCCACCTCCT TCTCAACAGC AGGGGTCACA GAGGAGACGG AAACACGGAG TTACACAGGG 
CAGAGGACAC CTGAGCTGGT GAATCGAGAG GCTGCTCTCC GTCAACAAAT TGAGCGTCTG 
AAGAGGGACC GGGCAGCCAT TTGCCTCCCG AAACCAAGCC CAGGAGTGGA GAGCAAACTG 
AGCCCTGAAA CCGGTCACCC AGCTGGGTCA CGAGGGGGAC ATGTGGTTAA AGATAACGTC 
AAACCTCCTG ATGTGAAGAA AGAGAAGGCT TCCCTCTTCT ACGAACTCAT TTCTGTCAGG 
GAGGAGATGT CAGATCTGCG AGCTCTGATC CGCCTGAAAG AGAAAGACCT GAGGTGTCTG 
GAGTGGAGTT TAATGGCCCA GAAGGCCCAG GAAGCAGCTG GAGTTTTCAT CCCTGAAAGT 
CTCAGAGAGG AGCTGGAGGA GGATCGTAAG ACTGAACAAC AGAGGCTCTG TGAGACCACA 
GCTAAACTGG GTGGTGATGG GGACATTGCC GGACCTCGAA CCAGGCCCAT TCTGAAAGAG 
CTCCAGGCCG TCCTGCAAAG GGAACAAGCC CTGAAGAAGA GATTAGCTTT AGTCCATGAC 
TCATTGAGCA CAGCTCTCTC AGACAGCGCC TCGCACAGGA GGGACAATGG AGAGCAGATT 
GCACGGCTCA CACAAGCTCA CAGTAAAGCC TTGAGCTCGT ATCGGCAGAT TCGCAGGAAG 
TATCGGGAGC AGGTATGGCG GCTGGAGCAG AAAGTGGTGG CCATGACGGA GAGTCACCAC 
AACCAGAGTG GAGCACCAAA AACGGCAGAG GAGGCCGTGG AGTGGAGGAG GGAAGAGACT 
ATTTTGTGA