frem1 gene cDNA ORF clone, Scleropages formosus(Asian bonytongue)

mRNA	Protein	Name
XM_018759627.1	XP_018615143.1	FRAS1-related extracellular matrix protein 1 isoform X4
XM_018759626.1	XP_018615142.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_018759624.2	XP_018615140.2	FRAS1-related extracellular matrix protein 1 isoform X1
XM_018759625.1	XP_018615141.1	FRAS1-related extracellular matrix protein 1 isoform X2
XM_018759629.1	XP_018615145.1	FRAS1-related extracellular matrix protein 1 isoform X6
XM_018759625.2	XP_018615141.2	FRAS1-related extracellular matrix protein 1 isoform X2
XM_018759626.2	XP_018615142.2	FRAS1-related extracellular matrix protein 1 isoform X3
XM_018759628.2	XP_018615144.2	FRAS1-related extracellular matrix protein 1 isoform X5
XM_029252146.1	XP_029107979.1	FRAS1-related extracellular matrix protein 1 isoform X6
XM_018759624.1	XP_018615140.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_018759628.1	XP_018615144.1	FRAS1-related extracellular matrix protein 1 isoform X5
XM_029252145.1	XP_029107978.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_018759627.2	XP_018615143.2	FRAS1-related extracellular matrix protein 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc125784	XM_029252146.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSc60384	XM_018759629.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OSc125779	XM_029252145.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSc125779	XM_018759624.2 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSc125780	XM_018759625.2 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSc125781	XM_018759626.2 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSc125782	XM_018759627.2 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSc125783	XM_018759628.2 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSc60379	XM_018759624.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSc60380	XM_018759625.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSc60381	XM_018759626.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSc60382	XM_018759627.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OSc60383	XM_018759628.1 Latest version!	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

frem1 ( XM_029252146.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029107979.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OSc125784
Clone ID Related Accession (Same CDS sequence)	XM_029252146.1
Accession Version	XM_029252146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041810.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGAA AAGTGACGCT GGAAGGTTGT TCTTGGTTGA CAGCATTCCC AAATTTGCCA 
AAGACCCCAG TGCACCCATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029107979.1
CDS	680..2803
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDRKVTLEGCSWLTAFPNLPKTPVHPF

Target ORF information:

RefSeq Version	XM_029252146.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029252146.1

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGAA AAGTGACGCT GGAAGGTTGT TCTTGGTTGA CAGCATTCCC AAATTTGCCA 
AAGACCCCAG TGCACCCATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759629.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615145.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSc60384
Clone ID Related Accession (Same CDS sequence)	XM_018759629.1
Accession Version	XM_018759629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372063.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG CCAGAGCAAA GTGTAAAGAG GCAGCTTGCC TGAAGGGAAT GAAACTGGTA 
CAAACGCTGA AGGTGCCATG TGAGGACTTC CTGCTAATGG GTCTGAGGTA CCAGCACACA 
AACCCTCCCT CACCAGATGT TGACTACATC GCCATAAGAG TGGACCTCAC AGATACAAGG 
AGCAGGAGCA TATACAGGTC AGAGCGGGCT TGGATTCCAG TGCGGATCAA AAATGCCATC 
CCAAACCAGC CACCCAAGCT GGCCTTTATG TCTACGTTCA TCTTGGAGGT GGACCAGTTT 
ATCCTCACCC CTTTCAGCAC TGCTGTCATT GATGCTGAGG ATGGGGAGAC CCCCAAGTCC 
CTGCTGGTGT TCAACATCAG CAAGCCCCCT CAGGAAGGAT TCATCACCCA CCTCTCTGAC 
CACACTAAGC CCATTACCTC CTTCCTCTGG GAGGACCTCA ATGACATGCA AATCGCCTAC 
CAGCCCCCCA ACTGCAGTCA CACACAACGT AGGAACTACG AGGTGGAGTT TGAAGTCCAT 
GATTTCTACT TTGTGAAGAG CACCCCAGTC GTGGTTCACA TATCAGTCAG GACTGCCGAC 
ACTAATGCAC CTCGAGTGTC TTGGAACATG GGCCTCAGTC TGCTGGAAGG TCAGTCGCGT 
CCTATCACAT GGGACCAGCT GCAGATTGCA GATAACAACA ACCTGAAATC AGTACGCATC 
ATCGTTGTTG ATGGGCTACA GCATGGAAGA CTCACGGTTA GAGGTGGTAA GGGCTTCATG 
TTCACTGTGA ATGACATCAA GGCTGGCGTG GTCCGCTACC ACCATGATGA CAGCGACACC 
ACCAAAGACT TTGTGGTCTT CCGGATCACT GATGGCTCAC ACCAAACGCG ACACAAGTTT 
CCCATTACCA TCCTCCCGAA AGACGACAGT CCACCTTTCC TCATCACCAA CACGGTGCTC 
GAGCTGGAAG AGGGCAATAC TGCCTTGCTG CGGGGGTCTG TACTGCAGGC TTCCGATATG 
GACTCCAGTG ACGACTATAT TTTGTTCAAC ATCACTCGTT CTCCCCAAGA TGGAGAAATC 
ATGAAAATAC CAGGCCCTGG GATCACCGGT TATCCTGTCA CCCGCTTCCT GCAGAGAGAT 
CTGTTCCACT CCATCATTTA TTACCGCCAC CTGGGAAATG AGGTCTTTGA GGACTCCTTT 
GAGGTGGTTC TCTCAGATTT TCATGACCCT CCCAATCTTT CAGAGCCTCA GGTTATTGTT 
GTCCACATTA CTCCTGTCCA CGATCAATTA CCGAAGGAGG CACCTGGGGT TACTCGACAA 
CTGATCGTTA AGGAGACAGA TGTAGTCCAC CTAACTAAGA AGCAGCTCCA TTTTATTGAC 
CTCGAGTCAC CTGACAGTGA GTTGACATAC ACTGTGACCA GTCCTCCCTT CTACATTAGC 
AGTCATGGGA AAAGTGACGC TGGAAGGTTG TTCTTGGTTG ACAGCATTCC CAAATTTGCC 
AAAGACCCCA GTGCACCCAT TTTAAGGCTG TTCACACAGC AAGCAGTGAA CTACATGAAG 
GTGGCCTACA TGCCACCCAT TGAGGACATT GGCATCCACC CCCAGCACAT CCAGTTTGTG 
CTCTCTGTCA CAAACCAGCA GGGTGGCACC CTTGTCGGGA TCTGCTTCAA CATCACTGTG 
CTCCCTGTAG ACAACCAGGC CCCTCAGGTC CACACCAGCC CCTTAGCTGT GGAAGAGGGT 
GGAGATTGTC CACTGGACTT GGATCACTTA CGGGTGACAG ACCCGGATTC GCCAAAGAAT 
GCCCTGTGGG TGGAGTTAAA GGTGGAGCCC CAGCACGGTC ACCTCTATCT GGATGGATCT 
GCCATAAGGC CAGGGCAGAC CTTCACACTG CAGGACATGA ATGGCCAAAC AGTTAGATAT 
CACCATGATG GCTCTGAGAC AGATCAGGAT GACATTGTTT TCATGGCTAC TGATGGGATC 
AACACTTCTG ACTTCATTCT GCACGTTGAG GTGGCGCTAG TCAATGATGA GGTTCCAGTT 
ATCATGCCAG GCCTGAAGCC TACGCTGGAT TGTGCCGAGG GACAGGAAGT GGTTATTACC 
ACGGAGTATC TCCAGGCCAC AGACAAGGAT AGTGACGACA GTGAACTGAT TTACATGATT 
GCTCGACAAC CCTATTATGG AGTGGTGTGT AAGAATGGCA TTGTGGTGGA CCGCTTTGTC 
CAGGCGGATG TGGTCACAGG GTCCATTACC TATAAGCACA CAGGACAGGA AATCGGACTG 
ACCCCTCGCT ATGACATCAT CACATTCGTC ATCTCAGACG AAGAGGCAGT GAATCTCCCA 
GCATGCACCT CCAGTGGCGG GCCAGCTGCC CATCTCCAGA AAGCCGTGCC TGTCTATGAC 
CTCAATGTCA CTGTTTTTCC TGTGGACAGC CAAGCCCCCT CACTTGTCAT TGGAGAGCTC 
TTTGTGGTAG ATGAAGGGGG TTTCGCCTCC ATCACCACAG CACACCTGAT TGCGTCCGAC 
GTCGACACCC CCGTTGGGGA GCTGGGGCTC ATCTTGGTAT CCCCCCCACA GTTTGGCTAC 
ATTGAAAACA TCTTGCCCAG TCCAGGGTTT GAGAAGAGTA ACATGGGGAT CAGCATTGGC 
TCCTTCACCT ACAGAGATGT GAAAAGCAGC TACATCAACT ACGTGCAGTC AAGGCACCAG 
AGACTGGAGC CAACAGCAGA CCAGTTCATG ATCAGAGTGT CCGATGGCAA GCGGAGCTCC 
CCTGACACTC CTTTCTACAT CATCATCAGC CCCACCAACG ATGAGGTTCC CGACTTCCTT 
GCCAGGAACA TCACGGTGAG TGAGGGACAG ATGAGGGAAC TGGACCCATC TATCCTCGAT 
GCGGTGGATC TAGATGTCCC GCGAGACCAC ATGGTGTTTA GCATCATTGA GTCTCCAAAG 
CACGGAGCGA TCATGGGCAA GCCCCATGTG AATGATGTCA ACCATCCCAG AACCCTCCGT 
CCCGGAAATC CAAACACTGG GGTTGAGATG AAGGATTTCA CCTTGGATGA GTTGAGGAAC 
GGGGTAACGG TCATGTATGT GCATGACGAC TCGGAGAGTC TGCATGACAG CTTCACCATC 
CAGGTGTCAG ATGGGAAGCA CCGGCTGCAA AAGCGGGTGG AGGTCACAGT TCTGCCCGTC 
AATGATGAGG CACCTCGTGT CACAAGAAAC ATGGGGATAG AGGTGGAGAT GGGCGAATCA 
AGGCTGATCT CCGGCGCTGT CCTCTCAGCC CAGGATGAGG ATACTCATCT CCAGAATGTC 
CTCTATGTGT TTGAGAGCAT TCCCACCCAG GGACTTCTGC AGATAAAGGT GGGCCTTGAC 
TGGGTGACAC TCGGAAAGGG GGCGAACTGC TCCCAGGAGA CTGTGGATGC CAACCTTTTC 
AGGTACCTTC ACACTGGCTC CACTGGGCCT CAGGAAGCCA CAGGGCTCCA AAACCAGGAC 
TTCTTCGTCT TCCATGTGGA GGATGGGGCA AACCGCTCAC CCACACAGAA CTTCCAGGTG 
ACACTGAAAA CCATGGAAAA GGGGGACATT GCAGTGTTCT CCAAACCGGT CCTTGTCACC 
CGGGGGGGCC GTGTGGTGCT GACCACAGAC ATCCTCCTGG CAGTGGATGG CACAGACCAC 
CCGGAGGAGC TGCTCTACGT GGTCATGGAG GGCCCAGCCC ACGGACACAT GGAGTACGCC 
CATCATCCTG GCACGCCCAT CACCTCCTTT AGCCAGATGG ATGTGGCCGC CAACCTGGTG 
TGCTACGTCC ACAGCAAGTG GGCCACGTCT CCAAGGGAGA TGCTGTGGTT CATGATCAGC 
AATGGGCATT CCTCCCGCAA CGGAACATTG GAGATCGCTG TGGAGACAGC TGACCGGGAA 
CTGCCTTCCT TACAACACAA TGGTGGCCTG TGGGTTCCCC AGGGATCCAC CACAGTGCTG 
TCCCCTGACG TGCTCACCCT GTCTGACCCT GACACCCCAC CGGATAGCCT CCTCTTCCTG 
CTGGTCCAGC CGCCACACTA CGGAGAGTTA CTCCTGAAGG GGGCGCCCCT ATACAGGGGA 
AACTTTACCC AGCAGCACCT CCTTGATCTG GACGTGGCCT ACAGACACGC AGGCAGCTCG 
GCACAGATCG ACCGCTTCTC CTTCATCGCA TCTGATGGCA CTAACCAGGG GTTCCTGCTG 
GAGGGGAAGC TGCAGACACA ACCAGTCTTC TTCACCATCC AGGTGGAGCC CCTGGACAGC 
CCTGCCCCCC AGATAATTCA GCTGCAGGCT GCTTGGAGGG CAGAGCTGCT GAAGGATGGC 
CGCTACGGCA TATTCGTCTC ATCTCGGGAG CTGAGAGCGA GGGAAGCAGG TGGCCGCAAC 
GAAAACATCA CCTTCCAGAT CATCCGAGGA CCAAGCTTCG GCCACCTGGA GAACGCCTCC 
ACAGGCCAGT TTATCCGCCA CGGCTTCACC CAGAGAGACC TGAGCAGGAG GATAGTCCTG 
TATGTCATCA GCCTGGAGAG TGAGGCTCTG TCTGACAGCC TGCAGTTTCA GGTCCTCAGC 
CCCCTTAGGA ACCACAGCCT GCCACACATA CTGGAATTCC ACTGGGCCAG CGTGGAGTTC 
TCCCAAACGA AGTTCAGCGT GTGTGAGGAG GAGGGAAGAC TATCGCTGAC CGTACTGAGG 
AGAGGAAACT GTGCCGAGTC CTCCTACGTG ACCGTCAAGG TTAAAGAAAT CACAGCTTCC 
TCTGGGGCAG ATTTCATCCC GAGTCCCGCA AGTTTAATTC AGTTTGATCC TGGAGTGTCC 
TTCAGAACCT GGTGGGTGGA GATTACCGAA GACCAACTGG AAGAGGCAGA GGAGATCTTC 
ACGGTGTCCC TGGTGTCACC CAAAGGCGTC GTACTAGGCC ACCGTGACAA CGCGCTTGTG 
ACCATTCTCG ATTCAGGGAC AGGCCAGTGC CGCACCAGTC GTAGAGTACA GCAGGTCCCT 
CCCTACCATG GGGCTATCCA GTTGGAGACC CTGCCCTTCG TCCGGGGTGA CGCACTACCC 
GTCGTGCAGC AGGAAACCCC CTTCAGCCAC AGGAGACTGA GGCCTGTGGG AAATGGAAGG 
ACGGTCCAAG CGTCTGTGGT TCAGCGAAAT GGATCCAATG TTGTTTTCAC ATATCATGGC 
ATGGTGTCAC TGCGAGTTCA GGACGACTCC TCTCCGTCCC AGCTGGCACG TTTGTCCCAG 
GTGAGGGTGA CCGGCAGAGG GAAGGAGAGA CCAGCTTCTG CTTCCGTGAG AGAGAATGCA 
GCGCTGGGAG CAGAACCTAG GGATGCCCCA TTCATCAAAC AGGACAGGGC AAGTCACTGG 
ATCCCCAGAG CATGTGGCCC TGGGGACACA GACCTGCTAC ACTACAACCG CAGCTCTGGC 
CAGATCTGGC ACTGCAATGG GGTCTCCTGG AAGCCGTGGA CCCCCAGAGA CCAGCAGCAA 
GACAAGTGCC CTCAAGGGTG GACGTTCCAC AGGGAGCGCT GCTACGTCCT GAGCAAGGGT 
CGGAGAGTCA CGTGGAGCGG TGCTGTGCGG GCCTGCAGGG AGATTTATCG CGGCACCCTG 
GTCAGCATTC GGTCAAAATC GGACATGGGA TGGCTGTGGG ACTTCGGTGG ACAGAGGCCC 
TTCTGGATCG GCCTGAACGA CCGGGAGAAC CGAGGCCGGT GGGAGTGGGT CGGGGGTGAG 
CCGGTGACCT ACACTAACTG GCGGGGACGT TCCCCCCGTG TGAAGAGGAA GGGGGGGACC 
AGGAGGTGCG TGCTGGTGCA GCAGCGAGCC AAGTGGAACA TGCGGGATTG TGAGAGGGGG 
AAGGAGCACC GCTTTGTGTG CCACACGCGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615145.1
CDS	280..6135
Translation	MNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTSRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759629.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759629.1

ORF Insert Sequence:

ATGAACAAAG CCAGAGCAAA GTGTAAAGAG GCAGCTTGCC TGAAGGGAAT GAAACTGGTA 
CAAACGCTGA AGGTGCCATG TGAGGACTTC CTGCTAATGG GTCTGAGGTA CCAGCACACA 
AACCCTCCCT CACCAGATGT TGACTACATC GCCATAAGAG TGGACCTCAC AGATACAAGG 
AGCAGGAGCA TATACAGGTC AGAGCGGGCT TGGATTCCAG TGCGGATCAA AAATGCCATC 
CCAAACCAGC CACCCAAGCT GGCCTTTATG TCTACGTTCA TCTTGGAGGT GGACCAGTTT 
ATCCTCACCC CTTTCAGCAC TGCTGTCATT GATGCTGAGG ATGGGGAGAC CCCCAAGTCC 
CTGCTGGTGT TCAACATCAG CAAGCCCCCT CAGGAAGGAT TCATCACCCA CCTCTCTGAC 
CACACTAAGC CCATTACCTC CTTCCTCTGG GAGGACCTCA ATGACATGCA AATCGCCTAC 
CAGCCCCCCA ACTGCAGTCA CACACAACGT AGGAACTACG AGGTGGAGTT TGAAGTCCAT 
GATTTCTACT TTGTGAAGAG CACCCCAGTC GTGGTTCACA TATCAGTCAG GACTGCCGAC 
ACTAATGCAC CTCGAGTGTC TTGGAACATG GGCCTCAGTC TGCTGGAAGG TCAGTCGCGT 
CCTATCACAT GGGACCAGCT GCAGATTGCA GATAACAACA ACCTGAAATC AGTACGCATC 
ATCGTTGTTG ATGGGCTACA GCATGGAAGA CTCACGGTTA GAGGTGGTAA GGGCTTCATG 
TTCACTGTGA ATGACATCAA GGCTGGCGTG GTCCGCTACC ACCATGATGA CAGCGACACC 
ACCAAAGACT TTGTGGTCTT CCGGATCACT GATGGCTCAC ACCAAACGCG ACACAAGTTT 
CCCATTACCA TCCTCCCGAA AGACGACAGT CCACCTTTCC TCATCACCAA CACGGTGCTC 
GAGCTGGAAG AGGGCAATAC TGCCTTGCTG CGGGGGTCTG TACTGCAGGC TTCCGATATG 
GACTCCAGTG ACGACTATAT TTTGTTCAAC ATCACTCGTT CTCCCCAAGA TGGAGAAATC 
ATGAAAATAC CAGGCCCTGG GATCACCGGT TATCCTGTCA CCCGCTTCCT GCAGAGAGAT 
CTGTTCCACT CCATCATTTA TTACCGCCAC CTGGGAAATG AGGTCTTTGA GGACTCCTTT 
GAGGTGGTTC TCTCAGATTT TCATGACCCT CCCAATCTTT CAGAGCCTCA GGTTATTGTT 
GTCCACATTA CTCCTGTCCA CGATCAATTA CCGAAGGAGG CACCTGGGGT TACTCGACAA 
CTGATCGTTA AGGAGACAGA TGTAGTCCAC CTAACTAAGA AGCAGCTCCA TTTTATTGAC 
CTCGAGTCAC CTGACAGTGA GTTGACATAC ACTGTGACCA GTCCTCCCTT CTACATTAGC 
AGTCATGGGA AAAGTGACGC TGGAAGGTTG TTCTTGGTTG ACAGCATTCC CAAATTTGCC 
AAAGACCCCA GTGCACCCAT TTTAAGGCTG TTCACACAGC AAGCAGTGAA CTACATGAAG 
GTGGCCTACA TGCCACCCAT TGAGGACATT GGCATCCACC CCCAGCACAT CCAGTTTGTG 
CTCTCTGTCA CAAACCAGCA GGGTGGCACC CTTGTCGGGA TCTGCTTCAA CATCACTGTG 
CTCCCTGTAG ACAACCAGGC CCCTCAGGTC CACACCAGCC CCTTAGCTGT GGAAGAGGGT 
GGAGATTGTC CACTGGACTT GGATCACTTA CGGGTGACAG ACCCGGATTC GCCAAAGAAT 
GCCCTGTGGG TGGAGTTAAA GGTGGAGCCC CAGCACGGTC ACCTCTATCT GGATGGATCT 
GCCATAAGGC CAGGGCAGAC CTTCACACTG CAGGACATGA ATGGCCAAAC AGTTAGATAT 
CACCATGATG GCTCTGAGAC AGATCAGGAT GACATTGTTT TCATGGCTAC TGATGGGATC 
AACACTTCTG ACTTCATTCT GCACGTTGAG GTGGCGCTAG TCAATGATGA GGTTCCAGTT 
ATCATGCCAG GCCTGAAGCC TACGCTGGAT TGTGCCGAGG GACAGGAAGT GGTTATTACC 
ACGGAGTATC TCCAGGCCAC AGACAAGGAT AGTGACGACA GTGAACTGAT TTACATGATT 
GCTCGACAAC CCTATTATGG AGTGGTGTGT AAGAATGGCA TTGTGGTGGA CCGCTTTGTC 
CAGGCGGATG TGGTCACAGG GTCCATTACC TATAAGCACA CAGGACAGGA AATCGGACTG 
ACCCCTCGCT ATGACATCAT CACATTCGTC ATCTCAGACG AAGAGGCAGT GAATCTCCCA 
GCATGCACCT CCAGTGGCGG GCCAGCTGCC CATCTCCAGA AAGCCGTGCC TGTCTATGAC 
CTCAATGTCA CTGTTTTTCC TGTGGACAGC CAAGCCCCCT CACTTGTCAT TGGAGAGCTC 
TTTGTGGTAG ATGAAGGGGG TTTCGCCTCC ATCACCACAG CACACCTGAT TGCGTCCGAC 
GTCGACACCC CCGTTGGGGA GCTGGGGCTC ATCTTGGTAT CCCCCCCACA GTTTGGCTAC 
ATTGAAAACA TCTTGCCCAG TCCAGGGTTT GAGAAGAGTA ACATGGGGAT CAGCATTGGC 
TCCTTCACCT ACAGAGATGT GAAAAGCAGC TACATCAACT ACGTGCAGTC AAGGCACCAG 
AGACTGGAGC CAACAGCAGA CCAGTTCATG ATCAGAGTGT CCGATGGCAA GCGGAGCTCC 
CCTGACACTC CTTTCTACAT CATCATCAGC CCCACCAACG ATGAGGTTCC CGACTTCCTT 
GCCAGGAACA TCACGGTGAG TGAGGGACAG ATGAGGGAAC TGGACCCATC TATCCTCGAT 
GCGGTGGATC TAGATGTCCC GCGAGACCAC ATGGTGTTTA GCATCATTGA GTCTCCAAAG 
CACGGAGCGA TCATGGGCAA GCCCCATGTG AATGATGTCA ACCATCCCAG AACCCTCCGT 
CCCGGAAATC CAAACACTGG GGTTGAGATG AAGGATTTCA CCTTGGATGA GTTGAGGAAC 
GGGGTAACGG TCATGTATGT GCATGACGAC TCGGAGAGTC TGCATGACAG CTTCACCATC 
CAGGTGTCAG ATGGGAAGCA CCGGCTGCAA AAGCGGGTGG AGGTCACAGT TCTGCCCGTC 
AATGATGAGG CACCTCGTGT CACAAGAAAC ATGGGGATAG AGGTGGAGAT GGGCGAATCA 
AGGCTGATCT CCGGCGCTGT CCTCTCAGCC CAGGATGAGG ATACTCATCT CCAGAATGTC 
CTCTATGTGT TTGAGAGCAT TCCCACCCAG GGACTTCTGC AGATAAAGGT GGGCCTTGAC 
TGGGTGACAC TCGGAAAGGG GGCGAACTGC TCCCAGGAGA CTGTGGATGC CAACCTTTTC 
AGGTACCTTC ACACTGGCTC CACTGGGCCT CAGGAAGCCA CAGGGCTCCA AAACCAGGAC 
TTCTTCGTCT TCCATGTGGA GGATGGGGCA AACCGCTCAC CCACACAGAA CTTCCAGGTG 
ACACTGAAAA CCATGGAAAA GGGGGACATT GCAGTGTTCT CCAAACCGGT CCTTGTCACC 
CGGGGGGGCC GTGTGGTGCT GACCACAGAC ATCCTCCTGG CAGTGGATGG CACAGACCAC 
CCGGAGGAGC TGCTCTACGT GGTCATGGAG GGCCCAGCCC ACGGACACAT GGAGTACGCC 
CATCATCCTG GCACGCCCAT CACCTCCTTT AGCCAGATGG ATGTGGCCGC CAACCTGGTG 
TGCTACGTCC ACAGCAAGTG GGCCACGTCT CCAAGGGAGA TGCTGTGGTT CATGATCAGC 
AATGGGCATT CCTCCCGCAA CGGAACATTG GAGATCGCTG TGGAGACAGC TGACCGGGAA 
CTGCCTTCCT TACAACACAA TGGTGGCCTG TGGGTTCCCC AGGGATCCAC CACAGTGCTG 
TCCCCTGACG TGCTCACCCT GTCTGACCCT GACACCCCAC CGGATAGCCT CCTCTTCCTG 
CTGGTCCAGC CGCCACACTA CGGAGAGTTA CTCCTGAAGG GGGCGCCCCT ATACAGGGGA 
AACTTTACCC AGCAGCACCT CCTTGATCTG GACGTGGCCT ACAGACACGC AGGCAGCTCG 
GCACAGATCG ACCGCTTCTC CTTCATCGCA TCTGATGGCA CTAACCAGGG GTTCCTGCTG 
GAGGGGAAGC TGCAGACACA ACCAGTCTTC TTCACCATCC AGGTGGAGCC CCTGGACAGC 
CCTGCCCCCC AGATAATTCA GCTGCAGGCT GCTTGGAGGG CAGAGCTGCT GAAGGATGGC 
CGCTACGGCA TATTCGTCTC ATCTCGGGAG CTGAGAGCGA GGGAAGCAGG TGGCCGCAAC 
GAAAACATCA CCTTCCAGAT CATCCGAGGA CCAAGCTTCG GCCACCTGGA GAACGCCTCC 
ACAGGCCAGT TTATCCGCCA CGGCTTCACC CAGAGAGACC TGAGCAGGAG GATAGTCCTG 
TATGTCATCA GCCTGGAGAG TGAGGCTCTG TCTGACAGCC TGCAGTTTCA GGTCCTCAGC 
CCCCTTAGGA ACCACAGCCT GCCACACATA CTGGAATTCC ACTGGGCCAG CGTGGAGTTC 
TCCCAAACGA AGTTCAGCGT GTGTGAGGAG GAGGGAAGAC TATCGCTGAC CGTACTGAGG 
AGAGGAAACT GTGCCGAGTC CTCCTACGTG ACCGTCAAGG TTAAAGAAAT CACAGCTTCC 
TCTGGGGCAG ATTTCATCCC GAGTCCCGCA AGTTTAATTC AGTTTGATCC TGGAGTGTCC 
TTCAGAACCT GGTGGGTGGA GATTACCGAA GACCAACTGG AAGAGGCAGA GGAGATCTTC 
ACGGTGTCCC TGGTGTCACC CAAAGGCGTC GTACTAGGCC ACCGTGACAA CGCGCTTGTG 
ACCATTCTCG ATTCAGGGAC AGGCCAGTGC CGCACCAGTC GTAGAGTACA GCAGGTCCCT 
CCCTACCATG GGGCTATCCA GTTGGAGACC CTGCCCTTCG TCCGGGGTGA CGCACTACCC 
GTCGTGCAGC AGGAAACCCC CTTCAGCCAC AGGAGACTGA GGCCTGTGGG AAATGGAAGG 
ACGGTCCAAG CGTCTGTGGT TCAGCGAAAT GGATCCAATG TTGTTTTCAC ATATCATGGC 
ATGGTGTCAC TGCGAGTTCA GGACGACTCC TCTCCGTCCC AGCTGGCACG TTTGTCCCAG 
GTGAGGGTGA CCGGCAGAGG GAAGGAGAGA CCAGCTTCTG CTTCCGTGAG AGAGAATGCA 
GCGCTGGGAG CAGAACCTAG GGATGCCCCA TTCATCAAAC AGGACAGGGC AAGTCACTGG 
ATCCCCAGAG CATGTGGCCC TGGGGACACA GACCTGCTAC ACTACAACCG CAGCTCTGGC 
CAGATCTGGC ACTGCAATGG GGTCTCCTGG AAGCCGTGGA CCCCCAGAGA CCAGCAGCAA 
GACAAGTGCC CTCAAGGGTG GACGTTCCAC AGGGAGCGCT GCTACGTCCT GAGCAAGGGT 
CGGAGAGTCA CGTGGAGCGG TGCTGTGCGG GCCTGCAGGG AGATTTATCG CGGCACCCTG 
GTCAGCATTC GGTCAAAATC GGACATGGGA TGGCTGTGGG ACTTCGGTGG ACAGAGGCCC 
TTCTGGATCG GCCTGAACGA CCGGGAGAAC CGAGGCCGGT GGGAGTGGGT CGGGGGTGAG 
CCGGTGACCT ACACTAACTG GCGGGGACGT TCCCCCCGTG TGAAGAGGAA GGGGGGGACC 
AGGAGGTGCG TGCTGGTGCA GCAGCGAGCC AAGTGGAACA TGCGGGATTG TGAGAGGGGG 
AAGGAGCACC GCTTTGTGTG CCACACGCGG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759624.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615140.2 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

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CloneID	OSc125779
Clone ID Related Accession (Same CDS sequence)	XM_018759624.2 , XM_029252145.1
Accession Version	XM_018759624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041810.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018759624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GGCCTGAACG ACCGGGAGAA CCGAGGCCGG TGGGAGTGGG TCGGGGGTGA GCCGGTGACC 
TACACTAACT GGCGGGGACG TTCCCCCCGT GTGAAGAGGA AGGGGGGGAC CAGGAGGTGC 
GTGCTGGTGC AGCAGCGAGC CAAGTGGAAC ATGCGGGATT GTGAGAGGGG GAAGGAGCAC 
CGCTTTGTGT GCCACACGCG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615140.2
CDS	680..7186
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTGRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759624.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759624.2

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GGCCTGAACG ACCGGGAGAA CCGAGGCCGG TGGGAGTGGG TCGGGGGTGA GCCGGTGACC 
TACACTAACT GGCGGGGACG TTCCCCCCGT GTGAAGAGGA AGGGGGGGAC CAGGAGGTGC 
GTGCTGGTGC AGCAGCGAGC CAAGTGGAAC ATGCGGGATT GTGAGAGGGG GAAGGAGCAC 
CGCTTTGTGT GCCACACGCG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_029252145.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_029107978.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

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CloneID	OSc125779
Clone ID Related Accession (Same CDS sequence)	XM_018759624.2 , XM_029252145.1
Accession Version	XM_029252145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041810.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GGCCTGAACG ACCGGGAGAA CCGAGGCCGG TGGGAGTGGG TCGGGGGTGA GCCGGTGACC 
TACACTAACT GGCGGGGACG TTCCCCCCGT GTGAAGAGGA AGGGGGGGAC CAGGAGGTGC 
GTGCTGGTGC AGCAGCGAGC CAAGTGGAAC ATGCGGGATT GTGAGAGGGG GAAGGAGCAC 
CGCTTTGTGT GCCACACGCG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029107978.1
CDS	338..6844
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTGRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_029252145.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029252145.1

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GGCCTGAACG ACCGGGAGAA CCGAGGCCGG TGGGAGTGGG TCGGGGGTGA GCCGGTGACC 
TACACTAACT GGCGGGGACG TTCCCCCCGT GTGAAGAGGA AGGGGGGGAC CAGGAGGTGC 
GTGCTGGTGC AGCAGCGAGC CAAGTGGAAC ATGCGGGATT GTGAGAGGGG GAAGGAGCAC 
CGCTTTGTGT GCCACACGCG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759625.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615141.2 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X3, mRNA.

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CloneID	OSc125780
Clone ID Related Accession (Same CDS sequence)	XM_018759625.2
Accession Version	XM_018759625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041810.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018759625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAAGA CAAGTGCCCT 
CAAGGGTGGA CGTTCCACAG GGAGCGCTGC TACGTCCTGA GCAAGGGTCG GAGAGTCACG 
TGGAGCGGTG CTGTGCGGGC CTGCAGGGAG ATTTATCGCG GCACCCTGGT CAGCATTCGG 
TCAAAATCGG ACATGGGATG GCTGTGGGAC TTCGGTGGAC AGAGGCCCTT CTGGATCGGC 
CTGAACGACC GGGAGAACCG AGGCCGGTGG GAGTGGGTCG GGGGTGAGCC GGTGACCTAC 
ACTAACTGGC GGGGACGTTC CCCCCGTGTG AAGAGGAAGG GGGGGACCAG GAGGTGCGTG 
CTGGTGCAGC AGCGAGCCAA GTGGAACATG CGGGATTGTG AGAGGGGGAA GGAGCACCGC 
TTTGTGTGCC ACACGCGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615141.2
CDS	680..7183
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTGRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759625.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759625.2

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAAGA CAAGTGCCCT 
CAAGGGTGGA CGTTCCACAG GGAGCGCTGC TACGTCCTGA GCAAGGGTCG GAGAGTCACG 
TGGAGCGGTG CTGTGCGGGC CTGCAGGGAG ATTTATCGCG GCACCCTGGT CAGCATTCGG 
TCAAAATCGG ACATGGGATG GCTGTGGGAC TTCGGTGGAC AGAGGCCCTT CTGGATCGGC 
CTGAACGACC GGGAGAACCG AGGCCGGTGG GAGTGGGTCG GGGGTGAGCC GGTGACCTAC 
ACTAACTGGC GGGGACGTTC CCCCCGTGTG AAGAGGAAGG GGGGGACCAG GAGGTGCGTG 
CTGGTGCAGC AGCGAGCCAA GTGGAACATG CGGGATTGTG AGAGGGGGAA GGAGCACCGC 
TTTGTGTGCC ACACGCGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759626.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615142.2 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.

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CloneID	OSc125781
Clone ID Related Accession (Same CDS sequence)	XM_018759626.2
Accession Version	XM_018759626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041810.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018759626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGACA GGGCAAGTCA CTGGATCCCC 
AGAGCATGTG GCCCTGGGGA CACAGACCTG CTACACTACA ACCGCAGCTC TGGCCAGATC 
TGGCACTGCA ATGGGGTCTC CTGGAAGCCG TGGACCCCCA GAGACCAGCA GCAAGACAAG 
TGCCCTCAAG GGTGGACGTT CCACAGGGAG CGCTGCTACG TCCTGAGCAA GGGTCGGAGA 
GTCACGTGGA GCGGTGCTGT GCGGGCCTGC AGGGAGATTT ATCGCGGCAC CCTGGTCAGC 
ATTCGGTCAA AATCGGACAT GGGATGGCTG TGGGACTTCG GTGGACAGAG GCCCTTCTGG 
ATCGGCCTGA ACGACCGGGA GAACCGAGGC CGGTGGGAGT GGGTCGGGGG TGAGCCGGTG 
ACCTACACTA ACTGGCGGGG ACGTTCCCCC CGTGTGAAGA GGAAGGGGGG GACCAGGAGG 
TGCGTGCTGG TGCAGCAGCG AGCCAAGTGG AACATGCGGG ATTGTGAGAG GGGGAAGGAG 
CACCGCTTTG TGTGCCACAC GCGGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615142.2
CDS	680..7129
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTGRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759626.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759626.2

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGACA GGGCAAGTCA CTGGATCCCC 
AGAGCATGTG GCCCTGGGGA CACAGACCTG CTACACTACA ACCGCAGCTC TGGCCAGATC 
TGGCACTGCA ATGGGGTCTC CTGGAAGCCG TGGACCCCCA GAGACCAGCA GCAAGACAAG 
TGCCCTCAAG GGTGGACGTT CCACAGGGAG CGCTGCTACG TCCTGAGCAA GGGTCGGAGA 
GTCACGTGGA GCGGTGCTGT GCGGGCCTGC AGGGAGATTT ATCGCGGCAC CCTGGTCAGC 
ATTCGGTCAA AATCGGACAT GGGATGGCTG TGGGACTTCG GTGGACAGAG GCCCTTCTGG 
ATCGGCCTGA ACGACCGGGA GAACCGAGGC CGGTGGGAGT GGGTCGGGGG TGAGCCGGTG 
ACCTACACTA ACTGGCGGGG ACGTTCCCCC CGTGTGAAGA GGAAGGGGGG GACCAGGAGG 
TGCGTGCTGG TGCAGCAGCG AGCCAAGTGG AACATGCGGG ATTGTGAGAG GGGGAAGGAG 
CACCGCTTTG TGTGCCACAC GCGGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759627.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615143.2 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.

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CloneID	OSc125782
Clone ID Related Accession (Same CDS sequence)	XM_018759627.2
Accession Version	XM_018759627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041810.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018759627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGACAGGGCA 
AGTCACTGGA TCCCCAGAGC ATGTGGCCCT GGGGACACAG ACCTGCTACA CTACAACCGC 
AGCTCTGGCC AGATCTGGCA CTGCAATGGG GTCTCCTGGA AGCCGTGGAC CCCCAGAGAC 
CAGCAGCAAG ACAAGTGCCC TCAAGGGTGG ACGTTCCACA GGGAGCGCTG CTACGTCCTG 
AGCAAGGGTC GGAGAGTCAC GTGGAGCGGT GCTGTGCGGG CCTGCAGGGA GATTTATCGC 
GGCACCCTGG TCAGCATTCG GTCAAAATCG GACATGGGAT GGCTGTGGGA CTTCGGTGGA 
CAGAGGCCCT TCTGGATCGG CCTGAACGAC CGGGAGAACC GAGGCCGGTG GGAGTGGGTC 
GGGGGTGAGC CGGTGACCTA CACTAACTGG CGGGGACGTT CCCCCCGTGT GAAGAGGAAG 
GGGGGGACCA GGAGGTGCGT GCTGGTGCAG CAGCGAGCCA AGTGGAACAT GCGGGATTGT 
GAGAGGGGGA AGGAGCACCG CTTTGTGTGC CACACGCGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615143.2
CDS	680..7084
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTGRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759627.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759627.2

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGACAGGGCA 
AGTCACTGGA TCCCCAGAGC ATGTGGCCCT GGGGACACAG ACCTGCTACA CTACAACCGC 
AGCTCTGGCC AGATCTGGCA CTGCAATGGG GTCTCCTGGA AGCCGTGGAC CCCCAGAGAC 
CAGCAGCAAG ACAAGTGCCC TCAAGGGTGG ACGTTCCACA GGGAGCGCTG CTACGTCCTG 
AGCAAGGGTC GGAGAGTCAC GTGGAGCGGT GCTGTGCGGG CCTGCAGGGA GATTTATCGC 
GGCACCCTGG TCAGCATTCG GTCAAAATCG GACATGGGAT GGCTGTGGGA CTTCGGTGGA 
CAGAGGCCCT TCTGGATCGG CCTGAACGAC CGGGAGAACC GAGGCCGGTG GGAGTGGGTC 
GGGGGTGAGC CGGTGACCTA CACTAACTGG CGGGGACGTT CCCCCCGTGT GAAGAGGAAG 
GGGGGGACCA GGAGGTGCGT GCTGGTGCAG CAGCGAGCCA AGTGGAACAT GCGGGATTGT 
GAGAGGGGGA AGGAGCACCG CTTTGTGTGC CACACGCGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759628.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615144.2 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X6, mRNA.

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CloneID	OSc125783
Clone ID Related Accession (Same CDS sequence)	XM_018759628.2
Accession Version	XM_018759628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041810.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018759628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GTTCTGTTAC CCACCGTTCC GCTGGCTGTC CTCTTCACTG CTCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615144.2
CDS	680..7030
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTGRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIVLLPTVPLAVLFTAQA

Target ORF information:

RefSeq Version	XM_018759628.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759628.2

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GAATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCGGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GTTCTGTTAC CCACCGTTCC GCTGGCTGTC CTCTTCACTG CTCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759624.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615140.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

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CloneID	OSc60379
Clone ID Related Accession (Same CDS sequence)	XM_018759624.1
Accession Version	XM_018759624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372063.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GGCCTGAACG ACCGGGAGAA CCGAGGCCGG TGGGAGTGGG TCGGGGGTGA GCCGGTGACC 
TACACTAACT GGCGGGGACG TTCCCCCCGT GTGAAGAGGA AGGGGGGGAC CAGGAGGTGC 
GTGCTGGTGC AGCAGCGAGC CAAGTGGAAC ATGCGGGATT GTGAGAGGGG GAAGGAGCAC 
CGCTTTGTGT GCCACACGCG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615140.1
CDS	680..7186
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTSRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759624.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759624.1

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GGCCTGAACG ACCGGGAGAA CCGAGGCCGG TGGGAGTGGG TCGGGGGTGA GCCGGTGACC 
TACACTAACT GGCGGGGACG TTCCCCCCGT GTGAAGAGGA AGGGGGGGAC CAGGAGGTGC 
GTGCTGGTGC AGCAGCGAGC CAAGTGGAAC ATGCGGGATT GTGAGAGGGG GAAGGAGCAC 
CGCTTTGTGT GCCACACGCG GACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759625.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615141.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

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CloneID	OSc60380
Clone ID Related Accession (Same CDS sequence)	XM_018759625.1
Accession Version	XM_018759625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372063.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAAGA CAAGTGCCCT 
CAAGGGTGGA CGTTCCACAG GGAGCGCTGC TACGTCCTGA GCAAGGGTCG GAGAGTCACG 
TGGAGCGGTG CTGTGCGGGC CTGCAGGGAG ATTTATCGCG GCACCCTGGT CAGCATTCGG 
TCAAAATCGG ACATGGGATG GCTGTGGGAC TTCGGTGGAC AGAGGCCCTT CTGGATCGGC 
CTGAACGACC GGGAGAACCG AGGCCGGTGG GAGTGGGTCG GGGGTGAGCC GGTGACCTAC 
ACTAACTGGC GGGGACGTTC CCCCCGTGTG AAGAGGAAGG GGGGGACCAG GAGGTGCGTG 
CTGGTGCAGC AGCGAGCCAA GTGGAACATG CGGGATTGTG AGAGGGGGAA GGAGCACCGC 
TTTGTGTGCC ACACGCGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615141.1
CDS	680..7183
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTSRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759625.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759625.1

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAAGA CAAGTGCCCT 
CAAGGGTGGA CGTTCCACAG GGAGCGCTGC TACGTCCTGA GCAAGGGTCG GAGAGTCACG 
TGGAGCGGTG CTGTGCGGGC CTGCAGGGAG ATTTATCGCG GCACCCTGGT CAGCATTCGG 
TCAAAATCGG ACATGGGATG GCTGTGGGAC TTCGGTGGAC AGAGGCCCTT CTGGATCGGC 
CTGAACGACC GGGAGAACCG AGGCCGGTGG GAGTGGGTCG GGGGTGAGCC GGTGACCTAC 
ACTAACTGGC GGGGACGTTC CCCCCGTGTG AAGAGGAAGG GGGGGACCAG GAGGTGCGTG 
CTGGTGCAGC AGCGAGCCAA GTGGAACATG CGGGATTGTG AGAGGGGGAA GGAGCACCGC 
TTTGTGTGCC ACACGCGGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759626.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615142.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X3, mRNA.

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CloneID	OSc60381
Clone ID Related Accession (Same CDS sequence)	XM_018759626.1
Accession Version	XM_018759626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372063.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGACA GGGCAAGTCA CTGGATCCCC 
AGAGCATGTG GCCCTGGGGA CACAGACCTG CTACACTACA ACCGCAGCTC TGGCCAGATC 
TGGCACTGCA ATGGGGTCTC CTGGAAGCCG TGGACCCCCA GAGACCAGCA GCAAGACAAG 
TGCCCTCAAG GGTGGACGTT CCACAGGGAG CGCTGCTACG TCCTGAGCAA GGGTCGGAGA 
GTCACGTGGA GCGGTGCTGT GCGGGCCTGC AGGGAGATTT ATCGCGGCAC CCTGGTCAGC 
ATTCGGTCAA AATCGGACAT GGGATGGCTG TGGGACTTCG GTGGACAGAG GCCCTTCTGG 
ATCGGCCTGA ACGACCGGGA GAACCGAGGC CGGTGGGAGT GGGTCGGGGG TGAGCCGGTG 
ACCTACACTA ACTGGCGGGG ACGTTCCCCC CGTGTGAAGA GGAAGGGGGG GACCAGGAGG 
TGCGTGCTGG TGCAGCAGCG AGCCAAGTGG AACATGCGGG ATTGTGAGAG GGGGAAGGAG 
CACCGCTTTG TGTGCCACAC GCGGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615142.1
CDS	680..7129
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTSRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759626.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759626.1

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGACA GGGCAAGTCA CTGGATCCCC 
AGAGCATGTG GCCCTGGGGA CACAGACCTG CTACACTACA ACCGCAGCTC TGGCCAGATC 
TGGCACTGCA ATGGGGTCTC CTGGAAGCCG TGGACCCCCA GAGACCAGCA GCAAGACAAG 
TGCCCTCAAG GGTGGACGTT CCACAGGGAG CGCTGCTACG TCCTGAGCAA GGGTCGGAGA 
GTCACGTGGA GCGGTGCTGT GCGGGCCTGC AGGGAGATTT ATCGCGGCAC CCTGGTCAGC 
ATTCGGTCAA AATCGGACAT GGGATGGCTG TGGGACTTCG GTGGACAGAG GCCCTTCTGG 
ATCGGCCTGA ACGACCGGGA GAACCGAGGC CGGTGGGAGT GGGTCGGGGG TGAGCCGGTG 
ACCTACACTA ACTGGCGGGG ACGTTCCCCC CGTGTGAAGA GGAAGGGGGG GACCAGGAGG 
TGCGTGCTGG TGCAGCAGCG AGCCAAGTGG AACATGCGGG ATTGTGAGAG GGGGAAGGAG 
CACCGCTTTG TGTGCCACAC GCGGACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759627.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615143.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.

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CloneID	OSc60382
Clone ID Related Accession (Same CDS sequence)	XM_018759627.1
Accession Version	XM_018759627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372063.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGACAGGGCA 
AGTCACTGGA TCCCCAGAGC ATGTGGCCCT GGGGACACAG ACCTGCTACA CTACAACCGC 
AGCTCTGGCC AGATCTGGCA CTGCAATGGG GTCTCCTGGA AGCCGTGGAC CCCCAGAGAC 
CAGCAGCAAG ACAAGTGCCC TCAAGGGTGG ACGTTCCACA GGGAGCGCTG CTACGTCCTG 
AGCAAGGGTC GGAGAGTCAC GTGGAGCGGT GCTGTGCGGG CCTGCAGGGA GATTTATCGC 
GGCACCCTGG TCAGCATTCG GTCAAAATCG GACATGGGAT GGCTGTGGGA CTTCGGTGGA 
CAGAGGCCCT TCTGGATCGG CCTGAACGAC CGGGAGAACC GAGGCCGGTG GGAGTGGGTC 
GGGGGTGAGC CGGTGACCTA CACTAACTGG CGGGGACGTT CCCCCCGTGT GAAGAGGAAG 
GGGGGGACCA GGAGGTGCGT GCTGGTGCAG CAGCGAGCCA AGTGGAACAT GCGGGATTGT 
GAGAGGGGGA AGGAGCACCG CTTTGTGTGC CACACGCGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615143.1
CDS	680..7084
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTSRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIGLNDRENRGRWEWVGGEPVTYTNWRGRSPRVKRKGGTRRCVLVQQRAKWNMRDCERGKEHRFVCHTRT

Target ORF information:

RefSeq Version	XM_018759627.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759627.1

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGACAGGGCA 
AGTCACTGGA TCCCCAGAGC ATGTGGCCCT GGGGACACAG ACCTGCTACA CTACAACCGC 
AGCTCTGGCC AGATCTGGCA CTGCAATGGG GTCTCCTGGA AGCCGTGGAC CCCCAGAGAC 
CAGCAGCAAG ACAAGTGCCC TCAAGGGTGG ACGTTCCACA GGGAGCGCTG CTACGTCCTG 
AGCAAGGGTC GGAGAGTCAC GTGGAGCGGT GCTGTGCGGG CCTGCAGGGA GATTTATCGC 
GGCACCCTGG TCAGCATTCG GTCAAAATCG GACATGGGAT GGCTGTGGGA CTTCGGTGGA 
CAGAGGCCCT TCTGGATCGG CCTGAACGAC CGGGAGAACC GAGGCCGGTG GGAGTGGGTC 
GGGGGTGAGC CGGTGACCTA CACTAACTGG CGGGGACGTT CCCCCCGTGT GAAGAGGAAG 
GGGGGGACCA GGAGGTGCGT GCTGGTGCAG CAGCGAGCCA AGTGGAACAT GCGGGATTGT 
GAGAGGGGGA AGGAGCACCG CTTTGTGTGC CACACGCGGA CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem1 ( XM_018759628.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018615144.1 Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.

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CloneID	OSc60383
Clone ID Related Accession (Same CDS sequence)	XM_018759628.1
Accession Version	XM_018759628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	FRAS1-related extracellular matrix protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017372063.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GTTCTGTTAC CCACCGTTCC GCTGGCTGTC CTCTTCACTG CTCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018615144.1
CDS	680..7030
Translation	MGSTGVRLPPLLLLLVLLVDMANMRRGSLVKVNRGLKVKRGQEAFLQEEDLRFHVLQERDTCKVEVVLNEPITQRVGSLTPQVFDCHFLTDEVKYVHNGCPILKEDRIMLRLYRFTEMETYTEIFFLHVEIQEPDCNIIHLGPRTLEVPEFYSVSDKLNGNVLSFHYERRHNLECNVRIATHDTHLPAHGHLVTGEPEKLEPRGDEPHSFILLQQQLMNKARAKCKEAACLKGMKLVQTLKVPCEDFLLMGLRYQHTNPPSPDVDYIAIRVDLTDTRSRSIYRSERAWIPVRIKNAIPNQPPKLAFMSTFILEVDQFILTPFSTAVIDAEDGETPKSLLVFNISKPPQEGFITHLSDHTKPITSFLWEDLNDMQIAYQPPNCSHTQRRNYEVEFEVHDFYFVKSTPVVVHISVRTADTNAPRVSWNMGLSLLEGQSRPITWDQLQIADNNNLKSVRIIVVDGLQHGRLTVRGGKGFMFTVNDIKAGVVRYHHDDSDTTKDFVVFRITDGSHQTRHKFPITILPKDDSPPFLITNTVLELEEGNTALLRGSVLQASDMDSSDDYILFNITRSPQDGEIMKIPGPGITGYPVTRFLQRDLFHSIIYYRHLGNEVFEDSFEVVLSDFHDPPNLSEPQVIVVHITPVHDQLPKEAPGVTRQLIVKETDVVHLTKKQLHFIDLESPDSELTYTVTSPPFYISSHGKSDAGRLFLVDSIPKFAKDPSAPILRLFTQQAVNYMKVAYMPPIEDIGIHPQHIQFVLSVTNQQGGTLVGICFNITVLPVDNQAPQVHTSPLAVEEGGDCPLDLDHLRVTDPDSPKNALWVELKVEPQHGHLYLDGSAIRPGQTFTLQDMNGQTVRYHHDGSETDQDDIVFMATDGINTSDFILHVEVALVNDEVPVIMPGLKPTLDCAEGQEVVITTEYLQATDKDSDDSELIYMIARQPYYGVVCKNGIVVDRFVQADVVTGSITYKHTGQEIGLTPRYDIITFVISDEEAVNLPACTSSGGPAAHLQKAVPVYDLNVTVFPVDSQAPSLVIGELFVVDEGGFASITTAHLIASDVDTPVGELGLILVSPPQFGYIENILPSPGFEKSNMGISIGSFTYRDVKSSYINYVQSRHQRLEPTADQFMIRVSDGKRSSPDTPFYIIISPTNDEVPDFLARNITVSEGQMRELDPSILDAVDLDVPRDHMVFSIIESPKHGAIMGKPHVNDVNHPRTLRPGNPNTGVEMKDFTLDELRNGVTVMYVHDDSESLHDSFTIQVSDGKHRLQKRVEVTVLPVNDEAPRVTRNMGIEVEMGESRLISGAVLSAQDEDTHLQNVLYVFESIPTQGLLQIKVGLDWVTLGKGANCSQETVDANLFRYLHTGSTGPQEATGLQNQDFFVFHVEDGANRSPTQNFQVTLKTMEKGDIAVFSKPVLVTRGGRVVLTTDILLAVDGTDHPEELLYVVMEGPAHGHMEYAHHPGTPITSFSQMDVAANLVCYVHSKWATSPREMLWFMISNGHSSRNGTLEIAVETADRELPSLQHNGGLWVPQGSTTVLSPDVLTLSDPDTPPDSLLFLLVQPPHYGELLLKGAPLYRGNFTQQHLLDLDVAYRHAGSSAQIDRFSFIASDGTNQGFLLEGKLQTQPVFFTIQVEPLDSPAPQIIQLQAAWRAELLKDGRYGIFVSSRELRAREAGGRNENITFQIIRGPSFGHLENASTGQFIRHGFTQRDLSRRIVLYVISLESEALSDSLQFQVLSPLRNHSLPHILEFHWASVEFSQTKFSVCEEEGRLSLTVLRRGNCAESSYVTVKVKEITASSGADFIPSPASLIQFDPGVSFRTWWVEITEDQLEEAEEIFTVSLVSPKGVVLGHRDNALVTILDSGTGQCRTSRRVQQVPPYHGAIQLETLPFVRGDALPVVQQETPFSHRRLRPVGNGRTVQASVVQRNGSNVVFTYHGMVSLRVQDDSSPSQLARLSQVRVTGRGKERPASASVRENAALGAEPRDAPFIKQDRASHWIPRACGPGDTDLLHYNRSSGQIWHCNGVSWKPWTPRDQQQDKCPQGWTFHRERCYVLSKGRRVTWSGAVRACREIYRGTLVSIRSKSDMGWLWDFGGQRPFWIVLLPTVPLAVLFTAQA

Target ORF information:

RefSeq Version	XM_018759628.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus FRAS1 related extracellular matrix 1 (frem1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018759628.1

ORF Insert Sequence:

ATGGGATCAA CAGGGGTTAG GTTGCCACCC CTGCTGTTGC TGTTGGTGTT GCTGGTGGAC 
ATGGCCAACA TGAGGCGGGG TTCCCTGGTG AAGGTGAATA GGGGTCTGAA GGTTAAGAGA 
GGCCAGGAGG CATTCCTGCA AGAAGAAGAC TTGCGCTTCC ACGTGCTCCA GGAGAGAGAC 
ACCTGCAAAG TGGAGGTGGT CCTCAATGAG CCCATCACAC AACGGGTGGG GTCACTCACA 
CCCCAGGTCT TTGATTGCCA CTTCTTGACG GATGAGGTGA AGTACGTTCA TAATGGCTGC 
CCGATCCTCA AAGAAGACAG GATTATGCTT CGGTTGTACA GGTTCACAGA GATGGAAACG 
TATACAGAGA TCTTCTTCCT GCACGTGGAG ATCCAGGAGC CTGACTGTAA CATCATCCAC 
TTGGGGCCCA GAACCTTGGA GGTGCCTGAG TTCTACAGTG TGTCAGACAA GCTGAATGGA 
AATGTCTTGT CCTTTCACTA CGAGCGACGA CACAACCTGG AGTGCAATGT GCGGATAGCT 
ACGCACGATA CCCACCTTCC TGCCCACGGG CACCTGGTCA CCGGTGAGCC TGAAAAGCTG 
GAGCCTCGAG GAGATGAACC ACACAGCTTC ATACTCCTCC AGCAGCAGCT AATGAACAAA 
GCCAGAGCAA AGTGTAAAGA GGCAGCTTGC CTGAAGGGAA TGAAACTGGT ACAAACGCTG 
AAGGTGCCAT GTGAGGACTT CCTGCTAATG GGTCTGAGGT ACCAGCACAC AAACCCTCCC 
TCACCAGATG TTGACTACAT CGCCATAAGA GTGGACCTCA CAGATACAAG GAGCAGGAGC 
ATATACAGGT CAGAGCGGGC TTGGATTCCA GTGCGGATCA AAAATGCCAT CCCAAACCAG 
CCACCCAAGC TGGCCTTTAT GTCTACGTTC ATCTTGGAGG TGGACCAGTT TATCCTCACC 
CCTTTCAGCA CTGCTGTCAT TGATGCTGAG GATGGGGAGA CCCCCAAGTC CCTGCTGGTG 
TTCAACATCA GCAAGCCCCC TCAGGAAGGA TTCATCACCC ACCTCTCTGA CCACACTAAG 
CCCATTACCT CCTTCCTCTG GGAGGACCTC AATGACATGC AAATCGCCTA CCAGCCCCCC 
AACTGCAGTC ACACACAACG TAGGAACTAC GAGGTGGAGT TTGAAGTCCA TGATTTCTAC 
TTTGTGAAGA GCACCCCAGT CGTGGTTCAC ATATCAGTCA GGACTGCCGA CACTAATGCA 
CCTCGAGTGT CTTGGAACAT GGGCCTCAGT CTGCTGGAAG GTCAGTCGCG TCCTATCACA 
TGGGACCAGC TGCAGATTGC AGATAACAAC AACCTGAAAT CAGTACGCAT CATCGTTGTT 
GATGGGCTAC AGCATGGAAG ACTCACGGTT AGAGGTGGTA AGGGCTTCAT GTTCACTGTG 
AATGACATCA AGGCTGGCGT GGTCCGCTAC CACCATGATG ACAGCGACAC CACCAAAGAC 
TTTGTGGTCT TCCGGATCAC TGATGGCTCA CACCAAACGC GACACAAGTT TCCCATTACC 
ATCCTCCCGA AAGACGACAG TCCACCTTTC CTCATCACCA ACACGGTGCT CGAGCTGGAA 
GAGGGCAATA CTGCCTTGCT GCGGGGGTCT GTACTGCAGG CTTCCGATAT GGACTCCAGT 
GACGACTATA TTTTGTTCAA CATCACTCGT TCTCCCCAAG ATGGAGAAAT CATGAAAATA 
CCAGGCCCTG GGATCACCGG TTATCCTGTC ACCCGCTTCC TGCAGAGAGA TCTGTTCCAC 
TCCATCATTT ATTACCGCCA CCTGGGAAAT GAGGTCTTTG AGGACTCCTT TGAGGTGGTT 
CTCTCAGATT TTCATGACCC TCCCAATCTT TCAGAGCCTC AGGTTATTGT TGTCCACATT 
ACTCCTGTCC ACGATCAATT ACCGAAGGAG GCACCTGGGG TTACTCGACA ACTGATCGTT 
AAGGAGACAG ATGTAGTCCA CCTAACTAAG AAGCAGCTCC ATTTTATTGA CCTCGAGTCA 
CCTGACAGTG AGTTGACATA CACTGTGACC AGTCCTCCCT TCTACATTAG CAGTCATGGG 
AAAAGTGACG CTGGAAGGTT GTTCTTGGTT GACAGCATTC CCAAATTTGC CAAAGACCCC 
AGTGCACCCA TTTTAAGGCT GTTCACACAG CAAGCAGTGA ACTACATGAA GGTGGCCTAC 
ATGCCACCCA TTGAGGACAT TGGCATCCAC CCCCAGCACA TCCAGTTTGT GCTCTCTGTC 
ACAAACCAGC AGGGTGGCAC CCTTGTCGGG ATCTGCTTCA ACATCACTGT GCTCCCTGTA 
GACAACCAGG CCCCTCAGGT CCACACCAGC CCCTTAGCTG TGGAAGAGGG TGGAGATTGT 
CCACTGGACT TGGATCACTT ACGGGTGACA GACCCGGATT CGCCAAAGAA TGCCCTGTGG 
GTGGAGTTAA AGGTGGAGCC CCAGCACGGT CACCTCTATC TGGATGGATC TGCCATAAGG 
CCAGGGCAGA CCTTCACACT GCAGGACATG AATGGCCAAA CAGTTAGATA TCACCATGAT 
GGCTCTGAGA CAGATCAGGA TGACATTGTT TTCATGGCTA CTGATGGGAT CAACACTTCT 
GACTTCATTC TGCACGTTGA GGTGGCGCTA GTCAATGATG AGGTTCCAGT TATCATGCCA 
GGCCTGAAGC CTACGCTGGA TTGTGCCGAG GGACAGGAAG TGGTTATTAC CACGGAGTAT 
CTCCAGGCCA CAGACAAGGA TAGTGACGAC AGTGAACTGA TTTACATGAT TGCTCGACAA 
CCCTATTATG GAGTGGTGTG TAAGAATGGC ATTGTGGTGG ACCGCTTTGT CCAGGCGGAT 
GTGGTCACAG GGTCCATTAC CTATAAGCAC ACAGGACAGG AAATCGGACT GACCCCTCGC 
TATGACATCA TCACATTCGT CATCTCAGAC GAAGAGGCAG TGAATCTCCC AGCATGCACC 
TCCAGTGGCG GGCCAGCTGC CCATCTCCAG AAAGCCGTGC CTGTCTATGA CCTCAATGTC 
ACTGTTTTTC CTGTGGACAG CCAAGCCCCC TCACTTGTCA TTGGAGAGCT CTTTGTGGTA 
GATGAAGGGG GTTTCGCCTC CATCACCACA GCACACCTGA TTGCGTCCGA CGTCGACACC 
CCCGTTGGGG AGCTGGGGCT CATCTTGGTA TCCCCCCCAC AGTTTGGCTA CATTGAAAAC 
ATCTTGCCCA GTCCAGGGTT TGAGAAGAGT AACATGGGGA TCAGCATTGG CTCCTTCACC 
TACAGAGATG TGAAAAGCAG CTACATCAAC TACGTGCAGT CAAGGCACCA GAGACTGGAG 
CCAACAGCAG ACCAGTTCAT GATCAGAGTG TCCGATGGCA AGCGGAGCTC CCCTGACACT 
CCTTTCTACA TCATCATCAG CCCCACCAAC GATGAGGTTC CCGACTTCCT TGCCAGGAAC 
ATCACGGTGA GTGAGGGACA GATGAGGGAA CTGGACCCAT CTATCCTCGA TGCGGTGGAT 
CTAGATGTCC CGCGAGACCA CATGGTGTTT AGCATCATTG AGTCTCCAAA GCACGGAGCG 
ATCATGGGCA AGCCCCATGT GAATGATGTC AACCATCCCA GAACCCTCCG TCCCGGAAAT 
CCAAACACTG GGGTTGAGAT GAAGGATTTC ACCTTGGATG AGTTGAGGAA CGGGGTAACG 
GTCATGTATG TGCATGACGA CTCGGAGAGT CTGCATGACA GCTTCACCAT CCAGGTGTCA 
GATGGGAAGC ACCGGCTGCA AAAGCGGGTG GAGGTCACAG TTCTGCCCGT CAATGATGAG 
GCACCTCGTG TCACAAGAAA CATGGGGATA GAGGTGGAGA TGGGCGAATC AAGGCTGATC 
TCCGGCGCTG TCCTCTCAGC CCAGGATGAG GATACTCATC TCCAGAATGT CCTCTATGTG 
TTTGAGAGCA TTCCCACCCA GGGACTTCTG CAGATAAAGG TGGGCCTTGA CTGGGTGACA 
CTCGGAAAGG GGGCGAACTG CTCCCAGGAG ACTGTGGATG CCAACCTTTT CAGGTACCTT 
CACACTGGCT CCACTGGGCC TCAGGAAGCC ACAGGGCTCC AAAACCAGGA CTTCTTCGTC 
TTCCATGTGG AGGATGGGGC AAACCGCTCA CCCACACAGA ACTTCCAGGT GACACTGAAA 
ACCATGGAAA AGGGGGACAT TGCAGTGTTC TCCAAACCGG TCCTTGTCAC CCGGGGGGGC 
CGTGTGGTGC TGACCACAGA CATCCTCCTG GCAGTGGATG GCACAGACCA CCCGGAGGAG 
CTGCTCTACG TGGTCATGGA GGGCCCAGCC CACGGACACA TGGAGTACGC CCATCATCCT 
GGCACGCCCA TCACCTCCTT TAGCCAGATG GATGTGGCCG CCAACCTGGT GTGCTACGTC 
CACAGCAAGT GGGCCACGTC TCCAAGGGAG ATGCTGTGGT TCATGATCAG CAATGGGCAT 
TCCTCCCGCA ACGGAACATT GGAGATCGCT GTGGAGACAG CTGACCGGGA ACTGCCTTCC 
TTACAACACA ATGGTGGCCT GTGGGTTCCC CAGGGATCCA CCACAGTGCT GTCCCCTGAC 
GTGCTCACCC TGTCTGACCC TGACACCCCA CCGGATAGCC TCCTCTTCCT GCTGGTCCAG 
CCGCCACACT ACGGAGAGTT ACTCCTGAAG GGGGCGCCCC TATACAGGGG AAACTTTACC 
CAGCAGCACC TCCTTGATCT GGACGTGGCC TACAGACACG CAGGCAGCTC GGCACAGATC 
GACCGCTTCT CCTTCATCGC ATCTGATGGC ACTAACCAGG GGTTCCTGCT GGAGGGGAAG 
CTGCAGACAC AACCAGTCTT CTTCACCATC CAGGTGGAGC CCCTGGACAG CCCTGCCCCC 
CAGATAATTC AGCTGCAGGC TGCTTGGAGG GCAGAGCTGC TGAAGGATGG CCGCTACGGC 
ATATTCGTCT CATCTCGGGA GCTGAGAGCG AGGGAAGCAG GTGGCCGCAA CGAAAACATC 
ACCTTCCAGA TCATCCGAGG ACCAAGCTTC GGCCACCTGG AGAACGCCTC CACAGGCCAG 
TTTATCCGCC ACGGCTTCAC CCAGAGAGAC CTGAGCAGGA GGATAGTCCT GTATGTCATC 
AGCCTGGAGA GTGAGGCTCT GTCTGACAGC CTGCAGTTTC AGGTCCTCAG CCCCCTTAGG 
AACCACAGCC TGCCACACAT ACTGGAATTC CACTGGGCCA GCGTGGAGTT CTCCCAAACG 
AAGTTCAGCG TGTGTGAGGA GGAGGGAAGA CTATCGCTGA CCGTACTGAG GAGAGGAAAC 
TGTGCCGAGT CCTCCTACGT GACCGTCAAG GTTAAAGAAA TCACAGCTTC CTCTGGGGCA 
GATTTCATCC CGAGTCCCGC AAGTTTAATT CAGTTTGATC CTGGAGTGTC CTTCAGAACC 
TGGTGGGTGG AGATTACCGA AGACCAACTG GAAGAGGCAG AGGAGATCTT CACGGTGTCC 
CTGGTGTCAC CCAAAGGCGT CGTACTAGGC CACCGTGACA ACGCGCTTGT GACCATTCTC 
GATTCAGGGA CAGGCCAGTG CCGCACCAGT CGTAGAGTAC AGCAGGTCCC TCCCTACCAT 
GGGGCTATCC AGTTGGAGAC CCTGCCCTTC GTCCGGGGTG ACGCACTACC CGTCGTGCAG 
CAGGAAACCC CCTTCAGCCA CAGGAGACTG AGGCCTGTGG GAAATGGAAG GACGGTCCAA 
GCGTCTGTGG TTCAGCGAAA TGGATCCAAT GTTGTTTTCA CATATCATGG CATGGTGTCA 
CTGCGAGTTC AGGACGACTC CTCTCCGTCC CAGCTGGCAC GTTTGTCCCA GGTGAGGGTG 
ACCGGCAGAG GGAAGGAGAG ACCAGCTTCT GCTTCCGTGA GAGAGAATGC AGCGCTGGGA 
GCAGAACCTA GGGATGCCCC ATTCATCAAA CAGGACAGGG CAAGTCACTG GATCCCCAGA 
GCATGTGGCC CTGGGGACAC AGACCTGCTA CACTACAACC GCAGCTCTGG CCAGATCTGG 
CACTGCAATG GGGTCTCCTG GAAGCCGTGG ACCCCCAGAG ACCAGCAGCA AGACAAGTGC 
CCTCAAGGGT GGACGTTCCA CAGGGAGCGC TGCTACGTCC TGAGCAAGGG TCGGAGAGTC 
ACGTGGAGCG GTGCTGTGCG GGCCTGCAGG GAGATTTATC GCGGCACCCT GGTCAGCATT 
CGGTCAAAAT CGGACATGGG ATGGCTGTGG GACTTCGGTG GACAGAGGCC CTTCTGGATC 
GTTCTGTTAC CCACCGTTCC GCTGGCTGTC CTCTTCACTG CTCAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	frem1
Entrez Gene ID	108938758
Full Name	FRAS1 related extracellular matrix 1
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

frem1 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene

Clones

gRNAs

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