col28a1 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	col28a1
Entrez Gene ID	108935658
Full Name	collagen type XXVIII alpha 1 chain
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018754439.1	XP_018609955.1	collagen alpha-1(XXVIII) chain
XM_018754439.2	XP_018609955.1	collagen alpha-1(XXVIII) chain

Pathways from BioSystems
Gene Ontology

The following col28a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col28a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSc56411	XM_018754439.2 Latest version!	Scleropages formosus collagen type XXVIII alpha 1 chain (col28a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OSc56411	XM_018754439.1	Scleropages formosus collagen type XXVIII alpha 1 chain (col28a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col28a1 ( XM_018754439.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018609955.1 Scleropages formosus collagen type XXVIII alpha 1 chain (col28a1), mRNA.

Price & Availability↑

CloneID	OSc56411
Clone ID Related Accession (Same CDS sequence)	XM_018754439.1 , XM_018754439.2
Accession Version	XM_018754439.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-1(XXVIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371894.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAG GCTTCTTAGT GTGTGTGCTG CTGCTGGCCA TTTCCGCTGC GGCGAGGGCC 
CAGGTGAGGA GAAGGAAAGG CCAAAGGGCC AGCAGCCTCG TTCTCCAGGA TGAAGAGAAG 
GGTACCGCGT GCGCGATGGA AATTGCATTT CTCCTGGACA GCTCTGAGAG TGCCAAGACA 
TTGCTGTTTG TGAAGCAGAG GGAGTTTGTC CTCAGCCTGG CAAAGCAGAT CGTGCAGATG 
CGGCTCAAGC CGCACGGGCG GGAACTGCGC GTCCGCCTGG CCGTCCTGCA GTACAGCAGC 
TCCGTCTCGG TGGAGCACAG CTTCCGCGAT TGGCAGGACC CGGACACGTT TGAGGAGCGG 
GTCAACGCCA TGGTGTACAT CGGCCACGGC ACGTACTCGT CCTACGCCGT GGGCAACGCC 
ACGCGGCTCT TCGTCGAAGA GACCAAGTCG GACAGCGTGC GGGTGGCACT GCTGATGACG 
GACGGTGCGG ATCACCCGCA GAGCCCAAAC GCGGTGGGCG CGGCTGCCGA GATGAAAGCC 
CGTGGCGTCA GGCTCTTCGC CGTCGGGCTG TCGGACCGGG CGAGGGAGGG GGTCAACAGC 
GCCAAGCTGC GCTCCATCGC CAGCAGCCCG CCGTCGCAGC ACGTGCTCAG CCTGACAGAC 
GCCTCGCTGG AGAAGAAACT TCTCAAGGAG CTTTCTGAAG TCGCCGCCGA GGGATGCCAG 
CAGCAGAAAA CTTGTTTGTG TGATAAAGGC GAGAGGGGAC CCCCTGGCTA CTCAGGAAAA 
AAGGGGGATC CTGGGGTTGA TGGAGCTCCT GGTTTAAGGG GACCAAAGGG GGAACCCGGA 
GCGAACGGCC GTCCGGGAAT CGAGGGCATT GAGGGAAGGC CAGGATTTAA AGGTGAAAAG 
GGCGAACGAG GTGAATGCGG GGCCCCCGGC CTGAAGGGCG AGCAGGGTAC GGAGGGACTG 
CAGGGACCCC GAGGACCCAG AGGAGAACAG GGCTCCCCTG GACCACCTGG AGATTTCGGT 
CCTGAGGGTC AGACTGGACC TAAAGGGGAT CGAGGACCCA GCGGCGTACC TGGGCCACCA 
GGGGACACTG GCATTGGATT TCCAGGCCCA AAGGGGGAGA AGGGAAACCA GGGTCGACCT 
GGTCCAGCTG GTCCCATTGC TGTAGGGGAG CCTGGCCTGC CTGGTTCTCC TGGGCCTCCA 
GGAGTACAAG GGCCCCAAGG CATTGCAGGG GAAGGTCTTC CCGGTTCAAA GGGGGATCGA 
GGGTATGATG GCATCAGAGG GCCACGTGGC CTGCCAGGCC CAGCGATAAA GGGAGATAAG 
GGGAACGTCG GACCTCCTGG ACCACCTGGC CCAACAGGAT TCCCAGGTTT CGGTATTCAA 
GGAGAAAAGG GGGTCCAAGG ACCAGCAGGA CCTCCAGGTC CTCGAGGGAT CCCTGGAGTG 
GGTCTAGCTG GATCAAAGGG CAGCCAGGGC TTTCCGGGCG AGCAGGGACC CCCGGGGGAA 
CGAGGAATTG GAGAGCCGGG GCCCAAAGGG GAACCTGGGC GAAACGGATC AGCCGGCATC 
CCGGGAATCC CAGGGGAAGA TGGGGCTGTT GGTGCTAAAG GAGAAATGGG GCTTCCCGGG 
TCCCGAGGGC CAGAAGGCAC TCCTGGGAAA GGTGTTCCTG GAGAAAAGGG GGACCGAGGA 
GACCGGGGTC TTAGGGGTCT GCCAGGAGTA GCGGGTGCTG TGGGTCCAGC GGGGGCCAAG 
GGAGAACCAG GAAGTATGGG TCTGATGGGA GTTCCTGGTC CACCGGGACG TGGTCTGCCT 
GGGGTCAAGG GCGAGCCGGG TCCAGCCGGG CCAGCCGGAC CAGTGGGGGA CCCTGGAATC 
GGATTGGCGG GCTCGAAGGG TGACCGTGGA TCACCAGGCC CTTTGGGACC ACCTGGACCA 
AAAGGGGATG GCTATTCTGG GCCTCAGGGG CCGCCAGGTG CACCGGGGCC ACCGGGAGAA 
ATCGGACCTG AAGGAATAGG TTTACCAGGC CCTAAAGGAG ACAGGGGCAC CCCTGGTCTT 
CCTGGGCCGT CCGGACCACC AGGAATCGGG TTCCAAGGAG CCAAGGGCTC CACTGGTCAA 
CCTGGTCCAC CAGGTCTTCA AGGTCCAGCT GGTGAAGGCA TCCAGGGACC AAAGGGAGAG 
CCTGGATTCC AAGGAACACC GGGTCCTCGA GGACCCCCAG GGGATGGCTT TCCCGGAGAA 
AAGGGAGACA GAGGCTTTAC TGGAGAACGA GGGAAGAAAG GAGAGAGGGG GGATTATGGA 
GAACCAGGAA GTACAGGACA AGTGGGAAGA CCTGGGCAGA AAGGAGAACC CGGTTTAACT 
AGAGAAGAAG TTATTAAAAT AATAAGGGAA ATATGTGGCT GTGGGATAAA ATGCCGGGAG 
AGCCCCCTGG AGCTCGTGTT CGTGATCGAC AGCTCAGAGA GCGTGGGACC CGAGAACTTC 
GACGTGGTGA AGGACTTTGT GAACGCCCTG ATCGACCGCG TATCCGTGAG CCGCGAAGCC 
ACGCGCATCG GCGTCGTCCT CTACAGCCAC ATCAGCATGG TGGTGGTGAG CCTGCAGCAG 
CAGTCCAGCC AGGATGACGT GAAGGCCGCC GTGCGCAAGA TGCCCTACTT GGGCGAGGGC 
ACATACACGG GCAGCGCCAT CAAACACGCC AACCAGGTGT TCCAGGCCTC TCGGCCCGGC 
GTGCGGAAGG TTGCCATCGT GCTCACCGAC GGCCAATCGG ACCGACGCGA CGAGGTGAAG 
CTGGAGGAGG CCGTACGCGA GGCGCACTCT GCCAGCGTTG AGATGTTCGT GATTGGGGTG 
GTGAACCGCA CTGACCCTCT CTACACAGAG TTTGCCAACG AAATGAATTC CATGGCCTCG 
GAGCCCAGCG ACGAGCACGT GTACCTGATC GACGACTTTA TGACGCTTCC TGCCCTGGAG 
AGCAAGCTCC TGAGCAGGAT CTGTGAAAGT GAGGATGGAA CGCTGTTTAG CCCCATCCCG 
AACTCCTTCC TCCCGCCCGG GACACCTGTC GTCACTGATT TGCGACCCCA GGAGATTCCC 
AAGAGGACTG ACACCCCCAC TTTCAATGGA GACTATGTCA GGATAAGCAA GGAGCCAGGA 
CCACCAGGGG AGCCATCGAT ACCACCTTTC CCCCAGGGTC CCAAGCCAGG TAGCCAGACC 
ACCCAAGCCA ACACAACCAG GACACTGAAC GAGGATGAAG AACGCCGTAA CAGCCTGACG 
GGTGACTTGG CAACGGCGCC TGTCAGCAGC GGAAAACGTC CTCTCCTGCC TGTCACCTCG 
GCGACAGGAG CACACATACC TGTGAACGAA GTGTTGGTTC TGGCGACAAG GCAGCTGTCC 
CCCACCCGCC TGCCTCCATC GACAGTGCAG GACAAATTCG TTTATGGAAA GGGCTGTGAG 
CAGGAGCTGG ACCCGGGCCC TTGCCGGCAG TACGTGGTAA AGTGGTACTA CGACAAGGCA 
GCCGATTCCT GTGCGCAGTT CTGGTTTGGT GGCTGCAAGG GCAACCAGAA CCAGTTTGAC 
ACGCAGGCCA GTTGTAGACA GGAATGCGTG CGAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018609955.1
CDS	229..3807
Translation	MGKGFLVCVLLLAISAAARAQVRRRKGQRASSLVLQDEEKGTACAMEIAFLLDSSESAKTLLFVKQREFVLSLAKQIVQMRLKPHGRELRVRLAVLQYSSSVSVEHSFRDWQDPDTFEERVNAMVYIGHGTYSSYAVGNATRLFVEETKSDSVRVALLMTDGADHPQSPNAVGAAAEMKARGVRLFAVGLSDRAREGVNSAKLRSIASSPPSQHVLSLTDASLEKKLLKELSEVAAEGCQQQKTCLCDKGERGPPGYSGKKGDPGVDGAPGLRGPKGEPGANGRPGIEGIEGRPGFKGEKGERGECGAPGLKGEQGTEGLQGPRGPRGEQGSPGPPGDFGPEGQTGPKGDRGPSGVPGPPGDTGIGFPGPKGEKGNQGRPGPAGPIAVGEPGLPGSPGPPGVQGPQGIAGEGLPGSKGDRGYDGIRGPRGLPGPAIKGDKGNVGPPGPPGPTGFPGFGIQGEKGVQGPAGPPGPRGIPGVGLAGSKGSQGFPGEQGPPGERGIGEPGPKGEPGRNGSAGIPGIPGEDGAVGAKGEMGLPGSRGPEGTPGKGVPGEKGDRGDRGLRGLPGVAGAVGPAGAKGEPGSMGLMGVPGPPGRGLPGVKGEPGPAGPAGPVGDPGIGLAGSKGDRGSPGPLGPPGPKGDGYSGPQGPPGAPGPPGEIGPEGIGLPGPKGDRGTPGLPGPSGPPGIGFQGAKGSTGQPGPPGLQGPAGEGIQGPKGEPGFQGTPGPRGPPGDGFPGEKGDRGFTGERGKKGERGDYGEPGSTGQVGRPGQKGEPGLTREEVIKIIREICGCGIKCRESPLELVFVIDSSESVGPENFDVVKDFVNALIDRVSVSREATRIGVVLYSHISMVVVSLQQQSSQDDVKAAVRKMPYLGEGTYTGSAIKHANQVFQASRPGVRKVAIVLTDGQSDRRDEVKLEEAVREAHSASVEMFVIGVVNRTDPLYTEFANEMNSMASEPSDEHVYLIDDFMTLPALESKLLSRICESEDGTLFSPIPNSFLPPGTPVVTDLRPQEIPKRTDTPTFNGDYVRISKEPGPPGEPSIPPFPQGPKPGSQTTQANTTRTLNEDEERRNSLTGDLATAPVSSGKRPLLPVTSATGAHIPVNEVLVLATRQLSPTRLPPSTVQDKFVYGKGCEQELDPGPCRQYVVKWYYDKAADSCAQFWFGGCKGNQNQFDTQASCRQECVRG

Target ORF information:

RefSeq Version	XM_018754439.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen type XXVIII alpha 1 chain (col28a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018754439.1

ORF Insert Sequence:

ATGGGAAAAG GCTTCTTAGT GTGTGTGCTG CTGCTGGCCA TTTCCGCTGC GGCGAGGGCC 
CAGGTGAGGA GAAGGAAAGG CCAAAGGGCC AGCAGCCTCG TTCTCCAGGA TGAAGAGAAG 
GGTACCGCGT GCGCGATGGA AATTGCATTT CTCCTGGACA GCTCTGAGAG TGCCAAGACA 
TTGCTGTTTG TGAAGCAGAG GGAGTTTGTC CTCAGCCTGG CAAAGCAGAT CGTGCAGATG 
CGGCTCAAGC CGCACGGGCG GGAACTGCGC GTCCGCCTGG CCGTCCTGCA GTACAGCAGC 
TCCGTCTCGG TGGAGCACAG CTTCCGCGAT TGGCAGGACC CGGACACGTT TGAGGAGCGG 
GTCAACGCCA TGGTGTACAT CGGCCACGGC ACGTACTCGT CCTACGCCGT GGGCAACGCC 
ACGCGGCTCT TCGTCGAAGA GACCAAGTCG GACAGCGTGC GGGTGGCACT GCTGATGACG 
GACGGTGCGG ATCACCCGCA GAGCCCAAAC GCGGTGGGCG CGGCTGCCGA GATGAAAGCC 
CGTGGCGTCA GGCTCTTCGC CGTCGGGCTG TCGGACCGGG CGAGGGAGGG GGTCAACAGC 
GCCAAGCTGC GCTCCATCGC CAGCAGCCCG CCGTCGCAGC ACGTGCTCAG CCTGACAGAC 
GCCTCGCTGG AGAAGAAACT TCTCAAGGAG CTTTCTGAAG TCGCCGCCGA GGGATGCCAG 
CAGCAGAAAA CTTGTTTGTG TGATAAAGGC GAGAGGGGAC CCCCTGGCTA CTCAGGAAAA 
AAGGGGGATC CTGGGGTTGA TGGAGCTCCT GGTTTAAGGG GACCAAAGGG GGAACCCGGA 
GCGAACGGCC GTCCGGGAAT CGAGGGCATT GAGGGAAGGC CAGGATTTAA AGGTGAAAAG 
GGCGAACGAG GTGAATGCGG GGCCCCCGGC CTGAAGGGCG AGCAGGGTAC GGAGGGACTG 
CAGGGACCCC GAGGACCCAG AGGAGAACAG GGCTCCCCTG GACCACCTGG AGATTTCGGT 
CCTGAGGGTC AGACTGGACC TAAAGGGGAT CGAGGACCCA GCGGCGTACC TGGGCCACCA 
GGGGACACTG GCATTGGATT TCCAGGCCCA AAGGGGGAGA AGGGAAACCA GGGTCGACCT 
GGTCCAGCTG GTCCCATTGC TGTAGGGGAG CCTGGCCTGC CTGGTTCTCC TGGGCCTCCA 
GGAGTACAAG GGCCCCAAGG CATTGCAGGG GAAGGTCTTC CCGGTTCAAA GGGGGATCGA 
GGGTATGATG GCATCAGAGG GCCACGTGGC CTGCCAGGCC CAGCGATAAA GGGAGATAAG 
GGGAACGTCG GACCTCCTGG ACCACCTGGC CCAACAGGAT TCCCAGGTTT CGGTATTCAA 
GGAGAAAAGG GGGTCCAAGG ACCAGCAGGA CCTCCAGGTC CTCGAGGGAT CCCTGGAGTG 
GGTCTAGCTG GATCAAAGGG CAGCCAGGGC TTTCCGGGCG AGCAGGGACC CCCGGGGGAA 
CGAGGAATTG GAGAGCCGGG GCCCAAAGGG GAACCTGGGC GAAACGGATC AGCCGGCATC 
CCGGGAATCC CAGGGGAAGA TGGGGCTGTT GGTGCTAAAG GAGAAATGGG GCTTCCCGGG 
TCCCGAGGGC CAGAAGGCAC TCCTGGGAAA GGTGTTCCTG GAGAAAAGGG GGACCGAGGA 
GACCGGGGTC TTAGGGGTCT GCCAGGAGTA GCGGGTGCTG TGGGTCCAGC GGGGGCCAAG 
GGAGAACCAG GAAGTATGGG TCTGATGGGA GTTCCTGGTC CACCGGGACG TGGTCTGCCT 
GGGGTCAAGG GCGAGCCGGG TCCAGCCGGG CCAGCCGGAC CAGTGGGGGA CCCTGGAATC 
GGATTGGCGG GCTCGAAGGG TGACCGTGGA TCACCAGGCC CTTTGGGACC ACCTGGACCA 
AAAGGGGATG GCTATTCTGG GCCTCAGGGG CCGCCAGGTG CACCGGGGCC ACCGGGAGAA 
ATCGGACCTG AAGGAATAGG TTTACCAGGC CCTAAAGGAG ACAGGGGCAC CCCTGGTCTT 
CCTGGGCCGT CCGGACCACC AGGAATCGGG TTCCAAGGAG CCAAGGGCTC CACTGGTCAA 
CCTGGTCCAC CAGGTCTTCA AGGTCCAGCT GGTGAAGGCA TCCAGGGACC AAAGGGAGAG 
CCTGGATTCC AAGGAACACC GGGTCCTCGA GGACCCCCAG GGGATGGCTT TCCCGGAGAA 
AAGGGAGACA GAGGCTTTAC TGGAGAACGA GGGAAGAAAG GAGAGAGGGG GGATTATGGA 
GAACCAGGAA GTACAGGACA AGTGGGAAGA CCTGGGCAGA AAGGAGAACC CGGTTTAACT 
AGAGAAGAAG TTATTAAAAT AATAAGGGAA ATATGTGGCT GTGGGATAAA ATGCCGGGAG 
AGCCCCCTGG AGCTCGTGTT CGTGATCGAC AGCTCAGAGA GCGTGGGACC CGAGAACTTC 
GACGTGGTGA AGGACTTTGT GAACGCCCTG ATCGACCGCG TATCCGTGAG CCGCGAAGCC 
ACGCGCATCG GCGTCGTCCT CTACAGCCAC ATCAGCATGG TGGTGGTGAG CCTGCAGCAG 
CAGTCCAGCC AGGATGACGT GAAGGCCGCC GTGCGCAAGA TGCCCTACTT GGGCGAGGGC 
ACATACACGG GCAGCGCCAT CAAACACGCC AACCAGGTGT TCCAGGCCTC TCGGCCCGGC 
GTGCGGAAGG TTGCCATCGT GCTCACCGAC GGCCAATCGG ACCGACGCGA CGAGGTGAAG 
CTGGAGGAGG CCGTACGCGA GGCGCACTCT GCCAGCGTTG AGATGTTCGT GATTGGGGTG 
GTGAACCGCA CTGACCCTCT CTACACAGAG TTTGCCAACG AAATGAATTC CATGGCCTCG 
GAGCCCAGCG ACGAGCACGT GTACCTGATC GACGACTTTA TGACGCTTCC TGCCCTGGAG 
AGCAAGCTCC TGAGCAGGAT CTGTGAAAGT GAGGATGGAA CGCTGTTTAG CCCCATCCCG 
AACTCCTTCC TCCCGCCCGG GACACCTGTC GTCACTGATT TGCGACCCCA GGAGATTCCC 
AAGAGGACTG ACACCCCCAC TTTCAATGGA GACTATGTCA GGATAAGCAA GGAGCCAGGA 
CCACCAGGGG AGCCATCGAT ACCACCTTTC CCCCAGGGTC CCAAGCCAGG TAGCCAGACC 
ACCCAAGCCA ACACAACCAG GACACTGAAC GAGGATGAAG AACGCCGTAA CAGCCTGACG 
GGTGACTTGG CAACGGCGCC TGTCAGCAGC GGAAAACGTC CTCTCCTGCC TGTCACCTCG 
GCGACAGGAG CACACATACC TGTGAACGAA GTGTTGGTTC TGGCGACAAG GCAGCTGTCC 
CCCACCCGCC TGCCTCCATC GACAGTGCAG GACAAATTCG TTTATGGAAA GGGCTGTGAG 
CAGGAGCTGG ACCCGGGCCC TTGCCGGCAG TACGTGGTAA AGTGGTACTA CGACAAGGCA 
GCCGATTCCT GTGCGCAGTT CTGGTTTGGT GGCTGCAAGG GCAACCAGAA CCAGTTTGAC 
ACGCAGGCCA GTTGTAGACA GGAATGCGTG CGAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col28a1 ( XM_018754439.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018609955.1 Scleropages formosus collagen type XXVIII alpha 1 chain (col28a1), mRNA.

Price & Availability↑

CloneID	OSc56411
Clone ID Related Accession (Same CDS sequence)	XM_018754439.1 , XM_018754439.2
Accession Version	XM_018754439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	collagen alpha-1(XXVIII) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041828.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018754439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAG GCTTCTTAGT GTGTGTGCTG CTGCTGGCCA TTTCCGCTGC GGCGAGGGCC 
CAGGTGAGGA GAAGGAAAGG CCAAAGGGCC AGCAGCCTCG TTCTCCAGGA TGAAGAGAAG 
GGTACCGCGT GCGCGATGGA AATTGCATTT CTCCTGGACA GCTCTGAGAG TGCCAAGACA 
TTGCTGTTTG TGAAGCAGAG GGAGTTTGTC CTCAGCCTGG CAAAGCAGAT CGTGCAGATG 
CGGCTCAAGC CGCACGGGCG GGAACTGCGC GTCCGCCTGG CCGTCCTGCA GTACAGCAGC 
TCCGTCTCGG TGGAGCACAG CTTCCGCGAT TGGCAGGACC CGGACACGTT TGAGGAGCGG 
GTCAACGCCA TGGTGTACAT CGGCCACGGC ACGTACTCGT CCTACGCCGT GGGCAACGCC 
ACGCGGCTCT TCGTCGAAGA GACCAAGTCG GACAGCGTGC GGGTGGCACT GCTGATGACG 
GACGGTGCGG ATCACCCGCA GAGCCCAAAC GCGGTGGGCG CGGCTGCCGA GATGAAAGCC 
CGTGGCGTCA GGCTCTTCGC CGTCGGGCTG TCGGACCGGG CGAGGGAGGG GGTCAACAGC 
GCCAAGCTGC GCTCCATCGC CAGCAGCCCG CCGTCGCAGC ACGTGCTCAG CCTGACAGAC 
GCCTCGCTGG AGAAGAAACT TCTCAAGGAG CTTTCTGAAG TCGCCGCCGA GGGATGCCAG 
CAGCAGAAAA CTTGTTTGTG TGATAAAGGC GAGAGGGGAC CCCCTGGCTA CTCAGGAAAA 
AAGGGGGATC CTGGGGTTGA TGGAGCTCCT GGTTTAAGGG GACCAAAGGG GGAACCCGGA 
GCGAACGGCC GTCCGGGAAT CGAGGGCATT GAGGGAAGGC CAGGATTTAA AGGTGAAAAG 
GGCGAACGAG GTGAATGCGG GGCCCCCGGC CTGAAGGGCG AGCAGGGTAC GGAGGGACTG 
CAGGGACCCC GAGGACCCAG AGGAGAACAG GGCTCCCCTG GACCACCTGG AGATTTCGGT 
CCTGAGGGTC AGACTGGACC TAAAGGGGAT CGAGGACCCA GCGGCGTACC TGGGCCACCA 
GGGGACACTG GCATTGGATT TCCAGGCCCA AAGGGGGAGA AGGGAAACCA GGGTCGACCT 
GGTCCAGCTG GTCCCATTGC TGTAGGGGAG CCTGGCCTGC CTGGTTCTCC TGGGCCTCCA 
GGAGTACAAG GGCCCCAAGG CATTGCAGGG GAAGGTCTTC CCGGTTCAAA GGGGGATCGA 
GGGTATGATG GCATCAGAGG GCCACGTGGC CTGCCAGGCC CAGCGATAAA GGGAGATAAG 
GGGAACGTCG GACCTCCTGG ACCACCTGGC CCAACAGGAT TCCCAGGTTT CGGTATTCAA 
GGAGAAAAGG GGGTCCAAGG ACCAGCAGGA CCTCCAGGTC CTCGAGGGAT CCCTGGAGTG 
GGTCTAGCTG GATCAAAGGG CAGCCAGGGC TTTCCGGGCG AGCAGGGACC CCCGGGGGAA 
CGAGGAATTG GAGAGCCGGG GCCCAAAGGG GAACCTGGGC GAAACGGATC AGCCGGCATC 
CCGGGAATCC CAGGGGAAGA TGGGGCTGTT GGTGCTAAAG GAGAAATGGG GCTTCCCGGG 
TCCCGAGGGC CAGAAGGCAC TCCTGGGAAA GGTGTTCCTG GAGAAAAGGG GGACCGAGGA 
GACCGGGGTC TTAGGGGTCT GCCAGGAGTA GCGGGTGCTG TGGGTCCAGC GGGGGCCAAG 
GGAGAACCAG GAAGTATGGG TCTGATGGGA GTTCCTGGTC CACCGGGACG TGGTCTGCCT 
GGGGTCAAGG GCGAGCCGGG TCCAGCCGGG CCAGCCGGAC CAGTGGGGGA CCCTGGAATC 
GGATTGGCGG GCTCGAAGGG TGACCGTGGA TCACCAGGCC CTTTGGGACC ACCTGGACCA 
AAAGGGGATG GCTATTCTGG GCCTCAGGGG CCGCCAGGTG CACCGGGGCC ACCGGGAGAA 
ATCGGACCTG AAGGAATAGG TTTACCAGGC CCTAAAGGAG ACAGGGGCAC CCCTGGTCTT 
CCTGGGCCGT CCGGACCACC AGGAATCGGG TTCCAAGGAG CCAAGGGCTC CACTGGTCAA 
CCTGGTCCAC CAGGTCTTCA AGGTCCAGCT GGTGAAGGCA TCCAGGGACC AAAGGGAGAG 
CCTGGATTCC AAGGAACACC GGGTCCTCGA GGACCCCCAG GGGATGGCTT TCCCGGAGAA 
AAGGGAGACA GAGGCTTTAC TGGAGAACGA GGGAAGAAAG GAGAGAGGGG GGATTATGGA 
GAACCAGGAA GTACAGGACA AGTGGGAAGA CCTGGGCAGA AAGGAGAACC CGGTTTAACT 
AGAGAAGAAG TTATTAAAAT AATAAGGGAA ATATGTGGCT GTGGGATAAA ATGCCGGGAG 
AGCCCCCTGG AGCTCGTGTT CGTGATCGAC AGCTCAGAGA GCGTGGGACC CGAGAACTTC 
GACGTGGTGA AGGACTTTGT GAACGCCCTG ATCGACCGCG TATCCGTGAG CCGCGAAGCC 
ACGCGCATCG GCGTCGTCCT CTACAGCCAC ATCAGCATGG TGGTGGTGAG CCTGCAGCAG 
CAGTCCAGCC AGGATGACGT GAAGGCCGCC GTGCGCAAGA TGCCCTACTT GGGCGAGGGC 
ACATACACGG GCAGCGCCAT CAAACACGCC AACCAGGTGT TCCAGGCCTC TCGGCCCGGC 
GTGCGGAAGG TTGCCATCGT GCTCACCGAC GGCCAATCGG ACCGACGCGA CGAGGTGAAG 
CTGGAGGAGG CCGTACGCGA GGCGCACTCT GCCAGCGTTG AGATGTTCGT GATTGGGGTG 
GTGAACCGCA CTGACCCTCT CTACACAGAG TTTGCCAACG AAATGAATTC CATGGCCTCG 
GAGCCCAGCG ACGAGCACGT GTACCTGATC GACGACTTTA TGACGCTTCC TGCCCTGGAG 
AGCAAGCTCC TGAGCAGGAT CTGTGAAAGT GAGGATGGAA CGCTGTTTAG CCCCATCCCG 
AACTCCTTCC TCCCGCCCGG GACACCTGTC GTCACTGATT TGCGACCCCA GGAGATTCCC 
AAGAGGACTG ACACCCCCAC TTTCAATGGA GACTATGTCA GGATAAGCAA GGAGCCAGGA 
CCACCAGGGG AGCCATCGAT ACCACCTTTC CCCCAGGGTC CCAAGCCAGG TAGCCAGACC 
ACCCAAGCCA ACACAACCAG GACACTGAAC GAGGATGAAG AACGCCGTAA CAGCCTGACG 
GGTGACTTGG CAACGGCGCC TGTCAGCAGC GGAAAACGTC CTCTCCTGCC TGTCACCTCG 
GCGACAGGAG CACACATACC TGTGAACGAA GTGTTGGTTC TGGCGACAAG GCAGCTGTCC 
CCCACCCGCC TGCCTCCATC GACAGTGCAG GACAAATTCG TTTATGGAAA GGGCTGTGAG 
CAGGAGCTGG ACCCGGGCCC TTGCCGGCAG TACGTGGTAA AGTGGTACTA CGACAAGGCA 
GCCGATTCCT GTGCGCAGTT CTGGTTTGGT GGCTGCAAGG GCAACCAGAA CCAGTTTGAC 
ACGCAGGCCA GTTGTAGACA GGAATGCGTG CGAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018609955.1
CDS	219..3797
Translation	MGKGFLVCVLLLAISAAARAQVRRRKGQRASSLVLQDEEKGTACAMEIAFLLDSSESAKTLLFVKQREFVLSLAKQIVQMRLKPHGRELRVRLAVLQYSSSVSVEHSFRDWQDPDTFEERVNAMVYIGHGTYSSYAVGNATRLFVEETKSDSVRVALLMTDGADHPQSPNAVGAAAEMKARGVRLFAVGLSDRAREGVNSAKLRSIASSPPSQHVLSLTDASLEKKLLKELSEVAAEGCQQQKTCLCDKGERGPPGYSGKKGDPGVDGAPGLRGPKGEPGANGRPGIEGIEGRPGFKGEKGERGECGAPGLKGEQGTEGLQGPRGPRGEQGSPGPPGDFGPEGQTGPKGDRGPSGVPGPPGDTGIGFPGPKGEKGNQGRPGPAGPIAVGEPGLPGSPGPPGVQGPQGIAGEGLPGSKGDRGYDGIRGPRGLPGPAIKGDKGNVGPPGPPGPTGFPGFGIQGEKGVQGPAGPPGPRGIPGVGLAGSKGSQGFPGEQGPPGERGIGEPGPKGEPGRNGSAGIPGIPGEDGAVGAKGEMGLPGSRGPEGTPGKGVPGEKGDRGDRGLRGLPGVAGAVGPAGAKGEPGSMGLMGVPGPPGRGLPGVKGEPGPAGPAGPVGDPGIGLAGSKGDRGSPGPLGPPGPKGDGYSGPQGPPGAPGPPGEIGPEGIGLPGPKGDRGTPGLPGPSGPPGIGFQGAKGSTGQPGPPGLQGPAGEGIQGPKGEPGFQGTPGPRGPPGDGFPGEKGDRGFTGERGKKGERGDYGEPGSTGQVGRPGQKGEPGLTREEVIKIIREICGCGIKCRESPLELVFVIDSSESVGPENFDVVKDFVNALIDRVSVSREATRIGVVLYSHISMVVVSLQQQSSQDDVKAAVRKMPYLGEGTYTGSAIKHANQVFQASRPGVRKVAIVLTDGQSDRRDEVKLEEAVREAHSASVEMFVIGVVNRTDPLYTEFANEMNSMASEPSDEHVYLIDDFMTLPALESKLLSRICESEDGTLFSPIPNSFLPPGTPVVTDLRPQEIPKRTDTPTFNGDYVRISKEPGPPGEPSIPPFPQGPKPGSQTTQANTTRTLNEDEERRNSLTGDLATAPVSSGKRPLLPVTSATGAHIPVNEVLVLATRQLSPTRLPPSTVQDKFVYGKGCEQELDPGPCRQYVVKWYYDKAADSCAQFWFGGCKGNQNQFDTQASCRQECVRG

Target ORF information:

RefSeq Version	XM_018754439.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus collagen type XXVIII alpha 1 chain (col28a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018754439.2

ORF Insert Sequence:

ATGGGAAAAG GCTTCTTAGT GTGTGTGCTG CTGCTGGCCA TTTCCGCTGC GGCGAGGGCC 
CAGGTGAGGA GAAGGAAAGG CCAAAGGGCC AGCAGCCTCG TTCTCCAGGA TGAAGAGAAG 
GGTACCGCGT GCGCGATGGA AATTGCATTT CTCCTGGACA GCTCTGAGAG TGCCAAGACA 
TTGCTGTTTG TGAAGCAGAG GGAGTTTGTC CTCAGCCTGG CAAAGCAGAT CGTGCAGATG 
CGGCTCAAGC CGCACGGGCG GGAACTGCGC GTCCGCCTGG CCGTCCTGCA GTACAGCAGC 
TCCGTCTCGG TGGAGCACAG CTTCCGCGAT TGGCAGGACC CGGACACGTT TGAGGAGCGG 
GTCAACGCCA TGGTGTACAT CGGCCACGGC ACGTACTCGT CCTACGCCGT GGGCAACGCC 
ACGCGGCTCT TCGTCGAAGA GACCAAGTCG GACAGCGTGC GGGTGGCACT GCTGATGACG 
GACGGTGCGG ATCACCCGCA GAGCCCAAAC GCGGTGGGCG CGGCTGCCGA GATGAAAGCC 
CGTGGCGTCA GGCTCTTCGC CGTCGGGCTG TCGGACCGGG CGAGGGAGGG GGTCAACAGC 
GCCAAGCTGC GCTCCATCGC CAGCAGCCCG CCGTCGCAGC ACGTGCTCAG CCTGACAGAC 
GCCTCGCTGG AGAAGAAACT TCTCAAGGAG CTTTCTGAAG TCGCCGCCGA GGGATGCCAG 
CAGCAGAAAA CTTGTTTGTG TGATAAAGGC GAGAGGGGAC CCCCTGGCTA CTCAGGAAAA 
AAGGGGGATC CTGGGGTTGA TGGAGCTCCT GGTTTAAGGG GACCAAAGGG GGAACCCGGA 
GCGAACGGCC GTCCGGGAAT CGAGGGCATT GAGGGAAGGC CAGGATTTAA AGGTGAAAAG 
GGCGAACGAG GTGAATGCGG GGCCCCCGGC CTGAAGGGCG AGCAGGGTAC GGAGGGACTG 
CAGGGACCCC GAGGACCCAG AGGAGAACAG GGCTCCCCTG GACCACCTGG AGATTTCGGT 
CCTGAGGGTC AGACTGGACC TAAAGGGGAT CGAGGACCCA GCGGCGTACC TGGGCCACCA 
GGGGACACTG GCATTGGATT TCCAGGCCCA AAGGGGGAGA AGGGAAACCA GGGTCGACCT 
GGTCCAGCTG GTCCCATTGC TGTAGGGGAG CCTGGCCTGC CTGGTTCTCC TGGGCCTCCA 
GGAGTACAAG GGCCCCAAGG CATTGCAGGG GAAGGTCTTC CCGGTTCAAA GGGGGATCGA 
GGGTATGATG GCATCAGAGG GCCACGTGGC CTGCCAGGCC CAGCGATAAA GGGAGATAAG 
GGGAACGTCG GACCTCCTGG ACCACCTGGC CCAACAGGAT TCCCAGGTTT CGGTATTCAA 
GGAGAAAAGG GGGTCCAAGG ACCAGCAGGA CCTCCAGGTC CTCGAGGGAT CCCTGGAGTG 
GGTCTAGCTG GATCAAAGGG CAGCCAGGGC TTTCCGGGCG AGCAGGGACC CCCGGGGGAA 
CGAGGAATTG GAGAGCCGGG GCCCAAAGGG GAACCTGGGC GAAACGGATC AGCCGGCATC 
CCGGGAATCC CAGGGGAAGA TGGGGCTGTT GGTGCTAAAG GAGAAATGGG GCTTCCCGGG 
TCCCGAGGGC CAGAAGGCAC TCCTGGGAAA GGTGTTCCTG GAGAAAAGGG GGACCGAGGA 
GACCGGGGTC TTAGGGGTCT GCCAGGAGTA GCGGGTGCTG TGGGTCCAGC GGGGGCCAAG 
GGAGAACCAG GAAGTATGGG TCTGATGGGA GTTCCTGGTC CACCGGGACG TGGTCTGCCT 
GGGGTCAAGG GCGAGCCGGG TCCAGCCGGG CCAGCCGGAC CAGTGGGGGA CCCTGGAATC 
GGATTGGCGG GCTCGAAGGG TGACCGTGGA TCACCAGGCC CTTTGGGACC ACCTGGACCA 
AAAGGGGATG GCTATTCTGG GCCTCAGGGG CCGCCAGGTG CACCGGGGCC ACCGGGAGAA 
ATCGGACCTG AAGGAATAGG TTTACCAGGC CCTAAAGGAG ACAGGGGCAC CCCTGGTCTT 
CCTGGGCCGT CCGGACCACC AGGAATCGGG TTCCAAGGAG CCAAGGGCTC CACTGGTCAA 
CCTGGTCCAC CAGGTCTTCA AGGTCCAGCT GGTGAAGGCA TCCAGGGACC AAAGGGAGAG 
CCTGGATTCC AAGGAACACC GGGTCCTCGA GGACCCCCAG GGGATGGCTT TCCCGGAGAA 
AAGGGAGACA GAGGCTTTAC TGGAGAACGA GGGAAGAAAG GAGAGAGGGG GGATTATGGA 
GAACCAGGAA GTACAGGACA AGTGGGAAGA CCTGGGCAGA AAGGAGAACC CGGTTTAACT 
AGAGAAGAAG TTATTAAAAT AATAAGGGAA ATATGTGGCT GTGGGATAAA ATGCCGGGAG 
AGCCCCCTGG AGCTCGTGTT CGTGATCGAC AGCTCAGAGA GCGTGGGACC CGAGAACTTC 
GACGTGGTGA AGGACTTTGT GAACGCCCTG ATCGACCGCG TATCCGTGAG CCGCGAAGCC 
ACGCGCATCG GCGTCGTCCT CTACAGCCAC ATCAGCATGG TGGTGGTGAG CCTGCAGCAG 
CAGTCCAGCC AGGATGACGT GAAGGCCGCC GTGCGCAAGA TGCCCTACTT GGGCGAGGGC 
ACATACACGG GCAGCGCCAT CAAACACGCC AACCAGGTGT TCCAGGCCTC TCGGCCCGGC 
GTGCGGAAGG TTGCCATCGT GCTCACCGAC GGCCAATCGG ACCGACGCGA CGAGGTGAAG 
CTGGAGGAGG CCGTACGCGA GGCGCACTCT GCCAGCGTTG AGATGTTCGT GATTGGGGTG 
GTGAACCGCA CTGACCCTCT CTACACAGAG TTTGCCAACG AAATGAATTC CATGGCCTCG 
GAGCCCAGCG ACGAGCACGT GTACCTGATC GACGACTTTA TGACGCTTCC TGCCCTGGAG 
AGCAAGCTCC TGAGCAGGAT CTGTGAAAGT GAGGATGGAA CGCTGTTTAG CCCCATCCCG 
AACTCCTTCC TCCCGCCCGG GACACCTGTC GTCACTGATT TGCGACCCCA GGAGATTCCC 
AAGAGGACTG ACACCCCCAC TTTCAATGGA GACTATGTCA GGATAAGCAA GGAGCCAGGA 
CCACCAGGGG AGCCATCGAT ACCACCTTTC CCCCAGGGTC CCAAGCCAGG TAGCCAGACC 
ACCCAAGCCA ACACAACCAG GACACTGAAC GAGGATGAAG AACGCCGTAA CAGCCTGACG 
GGTGACTTGG CAACGGCGCC TGTCAGCAGC GGAAAACGTC CTCTCCTGCC TGTCACCTCG 
GCGACAGGAG CACACATACC TGTGAACGAA GTGTTGGTTC TGGCGACAAG GCAGCTGTCC 
CCCACCCGCC TGCCTCCATC GACAGTGCAG GACAAATTCG TTTATGGAAA GGGCTGTGAG 
CAGGAGCTGG ACCCGGGCCC TTGCCGGCAG TACGTGGTAA AGTGGTACTA CGACAAGGCA 
GCCGATTCCT GTGCGCAGTT CTGGTTTGGT GGCTGCAAGG GCAACCAGAA CCAGTTTGAC 
ACGCAGGCCA GTTGTAGACA GGAATGCGTG CGAGGCTGA