crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJe33730	XM_030117130.1 Latest version!	Salarias fasciatus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_030117130.1 ) cDNA ORF clone, -> XP_029972990.1 Salarias fasciatus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OJe33730
Clone ID Related Accession (Same CDS sequence)	XM_030117130.1
Accession Version	XM_030117130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-26
Organism	Salarias fasciatus(jewelled blenny)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_043763.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Salarias fasciatus Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACA AATGCTCCGT GGTCTTCTTG AATGTCATCT TTCTGGCGCG GGCGCTCTCG 
GCACACGAGT GCACGCCGTG TAACCTCCGG GCATGTCCCG TCAAGCCCCC GCACCCCTGC 
GAAGGCGGCC GGACCTTGGC CAGGGACCCG TGCGGATGCT GCGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG GCAGGACTGG GCGCACGGCT ACTGCGCGCT GGGACTGACC 
TGCGCCTCTG TCAACAGAAC CGGAGCCGCC ACCGTCCCCG AGATCGGAGT GTGTAAAGTA 
CTTCCAAGCC ATCCAGAGGC GGGCCTCGAG GATGAGCGCT GCCCGTTGAT GACGGGCTGC 
GACAGGGCCG GAGGTCAGTG TGTATGCGAC GCCCGACACT CCTGCCTGGG CTCCTTCGCC 
TACCCGGACC AGGAAACCTG CATGAAGTCC AGTAAGTCAG ATGCTCGGAG GCACGAGCAC 
CGGGAACGGC ACAGGGAGAA GTACGTGGGG CCGTCCAACC CGGCGTGCAT GTTCTCCGGC 
TGTAACCTGA CCGCCGACGG CTGCGTGTGC GAGTCGCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGCGC AGGGGTGACG 
TGTCCCACTC TGGAGACACC CTCCTGCCCC AAGGGCTCGG TCCTGACCAG GAGTTACACC 
CCGCCCGCCG GTTGTTGCCC GTCCATACCA CCGCAGTGCA CCTGTGATCT CCGCGCCTGC 
CACAAGCCCC AGTGCCCGAG CGGACAGCAC CCGGTTCCAC TCAGTCAGGC CAGCGGGCAG 
CCAGGAGACT GCTGCGATGT CTTCGAGTGC CAGAAAGTTT CTCCCAAGTG CGTCCACAAC 
GGGAAGGAGT TCTCCGAAGG CGAGGTGTAC CGTATGGATC CCTGCTGGCT GTGTCAGTGC 
CGAGGAGGGA TCTCCTTCTG CTCCAAGGCA GAGTGTGCCG AGCTGGACTG CGAGAACTTC 
TACATCCCAG ACGGCGAGTG TTGCCCCGTC TGCATAGATG TGGAGCTGCT GTCGATGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCAGG CCCACGAGGA GCAGTGGAAG 
GAGGACGACT GCACCTTCTG CCAGTGCGTG GACGGCGAGC CGCACTGCAC CGCCATGGCC 
TGCAAGCAGA GCTGCCAGAA CCCCGTGAAG ATCCCCGGGG AGTGCTGTCC CTTCTGTGAA 
GAGCCCTCCT ATGAGACGGT CAGCCCCCTG CTGTGCCCCC CCCTGGAGAA CTGCTCCCTG 
TCGGTCAACG ACTGCCCCTT CGGCTTCCTG CAGGACAAGA ACGGCTGTCT CCTCTGCCAG 
TGCCTCAACA ACGACTCCTG CCCCGACCTG GAAAAATACT GCTCCCTGCA GTGCCCCATG 
GGATACGAGA GGGACGACTT CGGCTGCGAG GTGTGCGAGT GCAGCATCCC ATTGCCCAAG 
TGTCGACCTT TGACCTGCAC CAAGACCTGC CCCTACGGTT ATGTGAGGAA CAAGCACGGC 
TGTGAGATGT GTCGCTGCGT CAAGTGTCCT CCTTTCACCT GCGACAAGCA CTGCAGCAAC 
GGCTACCGCC AGAACAGGAA GGGCTGCTCC GTCTGCATGT GTAAAGAGAG CGTCCACGCC 
CCGAGCACGG TGGCCCCGGC CCCGACGCCC GGCTACTGCC TGACCTCCAA CGGGCACCGG 
TACGAGGAAG GGGACGGCTG GCACGACGGC TGCCGGGACT GCTACTGCCA CTCGGGACGG 
GAGATGTGTG TGCTCATATC CTGCCCGGTG CCCAGCTGCA CCAACCCCGT GGTCAAGCCG 
GAGCAGTGCT GTCCGACCTG CGAGGACGAG TCGGGCAGCG GTCAGCCAGA GGGGATGGAC 
ATGGTGGTGT GCCGCGCGCC CGGAGGGGAG TTCTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CGCGCTGCAC CTGCAGGAGG GGACGCGTCC TGTGCGACAC CGAAGTGTGT 
CCCCCGGCTC TCTGCCAGAC GCCCATCAGG AACAAGGACA CCTGTTGCCA TGTGTGCCCA 
GAGGAGACGC TGAGCCCCCT GCTCCCGGTG AACAACAGCC AGCAGGAGTA CTGCATCACC 
AGCGACGGAG ACGTGCTGCT CGCCGGGGAC TCCTGGAAGG CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ATGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCCCGCCGC CAACTGCAGG 
GTCCCCGTTT TACGCAAGGG CCAGTGCTGC CCACACTGTC TGGAAGTGAC CACGTCGGTG 
CCGGTGATGG TTTCTACCGG TGCGCCTGAG ACGACCGTCG CCGCCACCAC CGTGCAGAGC 
ACAGTGTGGA TCACCACCGT CACCGTACCG CCCATCATTG CCGAAACAGA TGAGCCAGGA 
TTGGGCGAGA ACTACCCCAG CCAGACAGAG ATGGCCCTCA TCTACCAATC AGCAGCCTGG 
ATCCTGGCAG GTCTCCTCTT GGCCATCATC ATCTTCCTCA TCGTGGCGTT GCTCATCAAC 
AGGAAGAAGA AGTGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC CGTCATCCTG 
AAGAAGCACG TCAACAAGAA CTCGGTGGTC TACATGGAGC CGTCCAAGGA GAACAAGTTC 
CAGAACGTGA AGAACGACTG CGGCATCATC CACTTCTCCC CGGACATGTC CTCCCCCTGC 
CTGGACAAGA TGACCATCCC CAGAGCCAAG CTGAGCAACG GCACCGACTG GACGCCGCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029972990.1
CDS	412..3234
Translation	MFNKCSVVFLNVIFLARALSAHECTPCNLRACPVKPPHPCEGGRTLARDPCGCCDQCARLEWEPCGGQDWAHGYCALGLTCASVNRTGAATVPEIGVCKVLPSHPEAGLEDERCPLMTGCDRAGGQCVCDARHSCLGSFAYPDQETCMKSSKSDARRHEHRERHREKYVGPSNPACMFSGCNLTADGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPTLETPSCPKGSVLTRSYTPPAGCCPSIPPQCTCDLRACHKPQCPSGQHPVPLSQASGQPGDCCDVFECQKVSPKCVHNGKEFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPDGECCPVCIDVELLSMDSTKASCWVNNKLQAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPLLCPPLENCSLSVNDCPFGFLQDKNGCLLCQCLNNDSCPDLEKYCSLQCPMGYERDDFGCEVCECSIPLPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSNGYRQNRKGCSVCMCKESVHAPSTVAPAPTPGYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCTNPVVKPEQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRRGRVLCDTEVCPPALCQTPIRNKDTCCHVCPEETLSPLLPVNNSQQEYCITSDGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLEVTTSVPVMVSTGAPETTVAATTVQSTVWITTVTVPPIIAETDEPGLGENYPSQTEMALIYQSAAWILAGLLLAIIIFLIVALLINRKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKNDCGIIHFSPDMSSPCLDKMTIPRAKLSNGTDWTPR

Target ORF information:

RefSeq Version	XM_030117130.1
Organism	Salarias fasciatus(jewelled blenny)
Definition	Salarias fasciatus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030117130.1

ORF Insert Sequence:

ATGTTCAACA AATGCTCCGT GGTCTTCTTG AATGTCATCT TTCTGGCGCG GGCGCTCTCG 
GCACACGAGT GCACGCCGTG TAACCTCCGG GCATGTCCCG TCAAGCCCCC GCACCCCTGC 
GAAGGCGGCC GGACCTTGGC CAGGGACCCG TGCGGATGCT GCGATCAGTG CGCGCGGCTG 
GAGTGGGAGC CGTGCGGCGG GCAGGACTGG GCGCACGGCT ACTGCGCGCT GGGACTGACC 
TGCGCCTCTG TCAACAGAAC CGGAGCCGCC ACCGTCCCCG AGATCGGAGT GTGTAAAGTA 
CTTCCAAGCC ATCCAGAGGC GGGCCTCGAG GATGAGCGCT GCCCGTTGAT GACGGGCTGC 
GACAGGGCCG GAGGTCAGTG TGTATGCGAC GCCCGACACT CCTGCCTGGG CTCCTTCGCC 
TACCCGGACC AGGAAACCTG CATGAAGTCC AGTAAGTCAG ATGCTCGGAG GCACGAGCAC 
CGGGAACGGC ACAGGGAGAA GTACGTGGGG CCGTCCAACC CGGCGTGCAT GTTCTCCGGC 
TGTAACCTGA CCGCCGACGG CTGCGTGTGC GAGTCGCAGA GCTGCCACCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCC GCAGAGGAGC TGAGGTGCGC AGGGGTGACG 
TGTCCCACTC TGGAGACACC CTCCTGCCCC AAGGGCTCGG TCCTGACCAG GAGTTACACC 
CCGCCCGCCG GTTGTTGCCC GTCCATACCA CCGCAGTGCA CCTGTGATCT CCGCGCCTGC 
CACAAGCCCC AGTGCCCGAG CGGACAGCAC CCGGTTCCAC TCAGTCAGGC CAGCGGGCAG 
CCAGGAGACT GCTGCGATGT CTTCGAGTGC CAGAAAGTTT CTCCCAAGTG CGTCCACAAC 
GGGAAGGAGT TCTCCGAAGG CGAGGTGTAC CGTATGGATC CCTGCTGGCT GTGTCAGTGC 
CGAGGAGGGA TCTCCTTCTG CTCCAAGGCA GAGTGTGCCG AGCTGGACTG CGAGAACTTC 
TACATCCCAG ACGGCGAGTG TTGCCCCGTC TGCATAGATG TGGAGCTGCT GTCGATGGAC 
AGCACCAAGG CCAGCTGCTG GGTGAACAAC AAGCTGCAGG CCCACGAGGA GCAGTGGAAG 
GAGGACGACT GCACCTTCTG CCAGTGCGTG GACGGCGAGC CGCACTGCAC CGCCATGGCC 
TGCAAGCAGA GCTGCCAGAA CCCCGTGAAG ATCCCCGGGG AGTGCTGTCC CTTCTGTGAA 
GAGCCCTCCT ATGAGACGGT CAGCCCCCTG CTGTGCCCCC CCCTGGAGAA CTGCTCCCTG 
TCGGTCAACG ACTGCCCCTT CGGCTTCCTG CAGGACAAGA ACGGCTGTCT CCTCTGCCAG 
TGCCTCAACA ACGACTCCTG CCCCGACCTG GAAAAATACT GCTCCCTGCA GTGCCCCATG 
GGATACGAGA GGGACGACTT CGGCTGCGAG GTGTGCGAGT GCAGCATCCC ATTGCCCAAG 
TGTCGACCTT TGACCTGCAC CAAGACCTGC CCCTACGGTT ATGTGAGGAA CAAGCACGGC 
TGTGAGATGT GTCGCTGCGT CAAGTGTCCT CCTTTCACCT GCGACAAGCA CTGCAGCAAC 
GGCTACCGCC AGAACAGGAA GGGCTGCTCC GTCTGCATGT GTAAAGAGAG CGTCCACGCC 
CCGAGCACGG TGGCCCCGGC CCCGACGCCC GGCTACTGCC TGACCTCCAA CGGGCACCGG 
TACGAGGAAG GGGACGGCTG GCACGACGGC TGCCGGGACT GCTACTGCCA CTCGGGACGG 
GAGATGTGTG TGCTCATATC CTGCCCGGTG CCCAGCTGCA CCAACCCCGT GGTCAAGCCG 
GAGCAGTGCT GTCCGACCTG CGAGGACGAG TCGGGCAGCG GTCAGCCAGA GGGGATGGAC 
ATGGTGGTGT GCCGCGCGCC CGGAGGGGAG TTCTACGTGG AGGGGGAGAC CTGGAACCTG 
GACGAGTGCA CGCGCTGCAC CTGCAGGAGG GGACGCGTCC TGTGCGACAC CGAAGTGTGT 
CCCCCGGCTC TCTGCCAGAC GCCCATCAGG AACAAGGACA CCTGTTGCCA TGTGTGCCCA 
GAGGAGACGC TGAGCCCCCT GCTCCCGGTG AACAACAGCC AGCAGGAGTA CTGCATCACC 
AGCGACGGAG ACGTGCTGCT CGCCGGGGAC TCCTGGAAGG CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ATGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCCCGCCGC CAACTGCAGG 
GTCCCCGTTT TACGCAAGGG CCAGTGCTGC CCACACTGTC TGGAAGTGAC CACGTCGGTG 
CCGGTGATGG TTTCTACCGG TGCGCCTGAG ACGACCGTCG CCGCCACCAC CGTGCAGAGC 
ACAGTGTGGA TCACCACCGT CACCGTACCG CCCATCATTG CCGAAACAGA TGAGCCAGGA 
TTGGGCGAGA ACTACCCCAG CCAGACAGAG ATGGCCCTCA TCTACCAATC AGCAGCCTGG 
ATCCTGGCAG GTCTCCTCTT GGCCATCATC ATCTTCCTCA TCGTGGCGTT GCTCATCAAC 
AGGAAGAAGA AGTGGGTGCA GATGTCCTGC TACAGCGCAC CAAAGAAGAC CGTCATCCTG 
AAGAAGCACG TCAACAAGAA CTCGGTGGTC TACATGGAGC CGTCCAAGGA GAACAAGTTC 
CAGAACGTGA AGAACGACTG CGGCATCATC CACTTCTCCC CGGACATGTC CTCCCCCTGC 
CTGGACAAGA TGACCATCCC CAGAGCCAAG CTGAGCAACG GCACCGACTG GACGCCGCGC 
TAG