Twist1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Twist1
Entrez Gene ID	85489
Full Name	twist family bHLH transcription factor 1
Synonyms	Twist
General protein information	Preferred Names twist family bHLH transcription factor 1 Names twist-related protein 1 twist basic helix-loop-helix transcription factor 1 twist gene homolog 1 twist homolog 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q16
Summary	beta helix loop helix transcription factor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053530.2	NP_445982.1	twist-related protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TWIST1	NP_001009050.2
Danio rerio (zebrafish)	twist1b	NP_001017820.1
Danio rerio (zebrafish)	LOC100535497	XP_003200219.1
Homo sapiens (human)	TWIST1	NP_000465.1
Rattus norvegicus (Norway rat)	Twist1	NP_445982.1
Mus musculus (house mouse)	Twist1	NP_035788.1
Gallus gallus (chicken)	TWIST1	NP_990070.1
Xenopus tropicalis (tropical clawed frog)	twist1	NP_989415.1
Danio rerio (zebrafish)	twist1a	NP_571059.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional Analysis of Two Novel Mutations in TWIST1 Protein Motifs Found in Ventricular Septal Defect Patients.
Deng X, Pan H, Wang J, Wang B, Cheng Z, Cheng L, Zhao L, Li H, Ma X
Pediatric cardiology36(8)1602-9(2015 Dec)

The hypoxia-inducible microRNA cluster miR-199a?214 targets myocardial PPAR? and impairs mitochondrial fatty acid oxidation.
el Azzouzi H, Leptidis S, Dirkx E, Hoeks J, van Bree B, Brand K, McClellan EA, Poels E, Sluimer JC, van den Hoogenhof MM, Armand AS, Yin X, Langley S, Bourajjaj M, Olieslagers S, Krishnan J, Vooijs M, Kurihara H, Stubbs A, Pinto YM, Krek W, Mayr M, da Costa Martins PA, Schrauwen P, De Windt LJ
Cell metabolism18(3)341-54(2013 Sep)

Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves.
Chakraborty S, Wirrig EE, Hinton RB, Merrill WH, Spicer DB, Yutzey KE
Developmental biology347(1)167-79(2010 Nov)

A possible inflammatory role of twist1 in human white adipocytes.
Pettersson AT, Laurencikiene J, Mejhert N, N?slund E, Bouloumi? A, Dahlman I, Arner P, Ryd?n M
Diabetes59(3)564-71(2010 Mar)

Gene expression profiling of pulmonary fibrosis identifies Twist1 as an antiapoptotic molecular "rectifier" of growth factor signaling.
Bridges RS, Kass D, Loh K, Glackin C, Borczuk AC, Greenberg S
The American journal of pathology175(6)2351-61(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Twist1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Twist1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09814	NM_053530.2 Latest version!	Rattus norvegicus twist family bHLH transcription factor 1 (Twist1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Twist1 ( NM_053530.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445982.1 Rattus norvegicus twist family bHLH transcription factor 1 (Twist1), mRNA.

Price & Availability↑

CloneID	ORa09814
Clone ID Related Accession (Same CDS sequence)	NM_053530.2
Accession Version	NM_053530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	twist-related protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ131845.1, BQ206289.1 and BF420349.1. On or before Jun 25, 2006 this sequence version replaced XM_001076553.1, NM_053530.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166412.1, DV714650.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END##

ATGATGCAGG ACGTGTCCAG CTCGCCAGTC TCGCCGGCCG ACGACAGCCT GAGCAACAGC 
GAGGAGGAGC CGGACCGGCA GCAGCCGGCG AGCGGCAAGC GGGGGGCTCG CAAGAGACGC 
AGCAGTCGGC GCAGCGCGGG CGGCAGCGCG GGGCCCGGCG GGGCCACGGG CGGGGGCATC 
GGAGGCGGCG ACGAGCCGGG CAGCCCGGCC CAGGGCAAGC GCGGCAAGAA ATCTGCGGGC 
GGAGGCGGCG GCGCGGGCGG AGGCGGCGGC GGCGGCGGCA GCAGCAGCGG GGGCGGGAGC 
CCGCAGTCGT ACGAGGAGCT GCAGACACAG CGGGTCATGG CCAACGTGCG GGAGCGCCAG 
CGCACCCAGT CGCTGAACGA GGCATTTGCC GCCCTGCGCA AGATCATCCC CACGCTGCCC 
TCGGACAAGC TGAGCAAGAT TCAGACCCTC AAACTGGCGG CCAGGTACAT CGACTTCCTG 
TACCAGGTCC TGCAGAGCGA CGAGCTGGAC TCCAAGATGG CAAGCTGCAG CTATGTGGCC 
CACGAGCGGC TCAGCTACGC CTTCTCCGTC TGGAGGATGG AGGGGGCCTG GTCCATGTCC 
GCGTCCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445982.1
CDS	179..790
Translation	MMQDVSSSPVSPADDSLSNSEEEPDRQQPASGKRGARKRRSSRRSAGGSAGPGGATGGGIGGGDEPGSPAQGKRGKKSAGGGGGAGGGGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH

Target ORF information:

RefSeq Version	NM_053530.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus twist family bHLH transcription factor 1 (Twist1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053530.2

ORF Insert Sequence:

ATGATGCAGG ACGTGTCCAG CTCGCCAGTC TCGCCGGCCG ACGACAGCCT GAGCAACAGC 
GAGGAGGAGC CGGACCGGCA GCAGCCGGCG AGCGGCAAGC GGGGGGCTCG CAAGAGACGC 
AGCAGTCGGC GCAGCGCGGG CGGCAGCGCG GGGCCCGGCG GGGCCACGGG CGGGGGCATC 
GGAGGCGGCG ACGAGCCGGG CAGCCCGGCC CAGGGCAAGC GCGGCAAGAA ATCTGCGGGC 
GGAGGCGGCG GCGCGGGCGG AGGCGGCGGC GGCGGCGGCA GCAGCAGCGG GGGCGGGAGC 
CCGCAGTCGT ACGAGGAGCT GCAGACACAG CGGGTCATGG CCAACGTGCG GGAGCGCCAG 
CGCACCCAGT CGCTGAACGA GGCATTTGCC GCCCTGCGCA AGATCATCCC CACGCTGCCC 
TCGGACAAGC TGAGCAAGAT TCAGACCCTC AAACTGGCGG CCAGGTACAT CGACTTCCTG 
TACCAGGTCC TGCAGAGCGA CGAGCTGGAC TCCAAGATGG CAAGCTGCAG CTATGTGGCC 
CACGAGCGGC TCAGCTACGC CTTCTCCGTC TGGAGGATGG AGGGGGCCTG GTCCATGTCC 
GCGTCCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional Analysis of Two Novel Mutations in TWIST1 Protein Motifs Found in Ventricular Septal Defect Patients.
Pediatric cardiology36(8)1602-9(2015 Dec)
Deng X,Pan H,Wang J,Wang B,Cheng Z,Cheng L,Zhao L,Li H,Ma X

The hypoxia-inducible microRNA cluster miR-199a?214 targets myocardial PPAR? and impairs mitochondrial fatty acid oxidation.
Cell metabolism18(3)341-54(2013 Sep)
el Azzouzi H,Leptidis S,Dirkx E,Hoeks J,van Bree B,Brand K,McClellan EA,Poels E,Sluimer JC,van den Hoogenhof MM,Armand AS,Yin X,Langley S,Bourajjaj M,Olieslagers S,Krishnan J,Vooijs M,Kurihara H,Stubbs A,Pinto YM,Krek W,Mayr M,da Costa Martins PA,Schrauwen P,De Windt LJ

Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves.
Developmental biology347(1)167-79(2010 Nov)
Chakraborty S,Wirrig EE,Hinton RB,Merrill WH,Spicer DB,Yutzey KE

A possible inflammatory role of twist1 in human white adipocytes.
Diabetes59(3)564-71(2010 Mar)
Pettersson AT,Laurencikiene J,Mejhert N,N?slund E,Bouloumi? A,Dahlman I,Arner P,Ryd?n M

Gene expression profiling of pulmonary fibrosis identifies Twist1 as an antiapoptotic molecular "rectifier" of growth factor signaling.
The American journal of pathology175(6)2351-61(2009 Dec)
Bridges RS,Kass D,Loh K,Glackin C,Borczuk AC,Greenberg S