Cd164 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd164
Entrez Gene ID	83689
Full Name	CD164 molecule
Synonyms	MGC-24v
General protein information	Preferred Names CD164 molecule Names sialomucin core protein 24 CD164 antigen CD164 molecule, sialomucin MGC-24 MUC-24 endolyn multi-glycosylated core protein 24
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q12
Summary	membrane protein found in lysosomal and endosomal compartments [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031812.1	NP_114000.1	sialomucin core protein 24 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cd164	NP_114000.1
Homo sapiens (human)	CD164	NP_006007.2
	CD164	XP_001091286.1
Mus musculus (house mouse)	Cd164	NP_058594.1
Canis lupus familiaris (dog)	CD164	XP_532256.2
Bos taurus (cattle)	CD164	NP_001039506.1

Gene Ontology
Bibliography

Related articles in PubMed

A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
Nyegaard M, Rendtorff ND, Nielsen MS, Corydon TJ, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard MT, Leal SM, Ahmad W, Wikman FP, Petersen KB, Cr?ger DG, Oostrik J, Kremer H, Tommerup N, Fr?din M, Steel KP, Tranebj?rg L, B?rglum AD
PLoS genetics11(7)e1005386(2015 Jul)

Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.
Mo D, Costa SA, Ihrke G, Youker RT, Pastor-Soler N, Hughey RP, Weisz OA
Molecular biology of the cell23(18)3636-46(2012 Sep)

Specific N-glycans direct apical delivery of transmembrane, but not soluble or glycosylphosphatidylinositol-anchored forms of endolyn in Madin-Darby canine kidney cells.
Potter BA, Ihrke G, Bruns JR, Weixel KM, Weisz OA
Molecular biology of the cell15(3)1407-16(2004 Mar)

Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn.
Chan JY, Lee-Prudhoe JE, Jorgensen B, Ihrke G, Doyonnas R, Zannettino AC, Buckle VJ, Ward CJ, Simmons PJ, Watt SM
The Journal of biological chemistry276(3)2139-52(2001 Jan)

Endolyn is a mucin-like type I membrane protein targeted to lysosomes by its cytoplasmic tail.
Ihrke G, Gray SR, Luzio JP
The Biochemical journal345 Pt 2287-96(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that galectin-9 plays a role in apical sorting of endolyn.
Title: Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.

Specific N-glycans are only essential for the apical sorting of transmembrane endolyn, suggesting fundamental differences in the mechanisms by which soluble, glycosylphosphatidylinositol-anchored, and transmembrane proteins are sorted.
Title: Specific N-glycans direct apical delivery of transmembrane, but not soluble or glycosylphosphatidylinositol-anchored forms of endolyn in Madin-Darby canine kidney cells.

The following Cd164 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd164 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08792	NM_031812.1 Latest version!	Rattus norvegicus CD164 molecule (Cd164), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd164 ( NM_031812.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114000.1 Rattus norvegicus CD164 molecule (Cd164), mRNA.

Price & Availability↑

CloneID	ORa08792
Clone ID Related Accession (Same CDS sequence)	NM_031812.1
Accession Version	NM_031812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	sialomucin core protein 24 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ238574.1. ##Evidence-Data-START## Transcript exon combination :: AJ238574.1, BC062064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGCG CCTCCCGCGG ACTGTTCTGG GCCGCCACCT GCCTCGCCGC GCTCTGCCTG 
TCGGCCGCGC AGAGCAACAG CAGCGCATCT CCCAACGTGA CTGACCCGCC GACCACGACC 
AGCAAAGTGG TCCCGACGAC GCTCACCACC ACCAAGCCGC CAGAAACCTG TGAGAGCTTC 
AACAGCTGTG TTTCCTGTGT CAACGCCACC TTGACTAATA ATATTACCTG CGTCTGGCTA 
GATTGCCATG AAGCAAATAA GACCTATTGT TCAAGTGAAT TAGTAAGTAA TTGTACCCAG 
AAGACCAGTA CTGACTCCTG TTCTGTAATA CCTACCACCC CAGTGCCAAC CAATTCTACA 
GCTAAGCCTA CAACTCGGCC TTCCTCTCCT ACACCTACTC CCTCAGTTGT CACATCAGCA 
GGTGCAACAA ATACCACTGT GACTCCAACC TCACAGCCTG AGCGGAAGTC CACCTTTGAT 
GCAGCCAGCT TCATTGGAGG AATCGTCCTT GTCTTGGGTG TGCAGGCTGT AATTTTCTTT 
CTCTATAAAT TCTGCAAATC TAAAGAACGA AATTACCATA CTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114000.1
CDS	15..602
Translation	MSGASRGLFWAATCLAALCLSAAQSNSSASPNVTDPPTTTSKVVPTTLTTTKPPETCESFNSCVSCVNATLTNNITCVWLDCHEANKTYCSSELVSNCTQKTSTDSCSVIPTTPVPTNSTAKPTTRPSSPTPTPSVVTSAGATNTTVTPTSQPERKSTFDAASFIGGIVLVLGVQAVIFFLYKFCKSKERNYHTL

Target ORF information:

RefSeq Version	NM_031812.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CD164 molecule (Cd164), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031812.1

ORF Insert Sequence:

ATGTCGGGCG CCTCCCGCGG ACTGTTCTGG GCCGCCACCT GCCTCGCCGC GCTCTGCCTG 
TCGGCCGCGC AGAGCAACAG CAGCGCATCT CCCAACGTGA CTGACCCGCC GACCACGACC 
AGCAAAGTGG TCCCGACGAC GCTCACCACC ACCAAGCCGC CAGAAACCTG TGAGAGCTTC 
AACAGCTGTG TTTCCTGTGT CAACGCCACC TTGACTAATA ATATTACCTG CGTCTGGCTA 
GATTGCCATG AAGCAAATAA GACCTATTGT TCAAGTGAAT TAGTAAGTAA TTGTACCCAG 
AAGACCAGTA CTGACTCCTG TTCTGTAATA CCTACCACCC CAGTGCCAAC CAATTCTACA 
GCTAAGCCTA CAACTCGGCC TTCCTCTCCT ACACCTACTC CCTCAGTTGT CACATCAGCA 
GGTGCAACAA ATACCACTGT GACTCCAACC TCACAGCCTG AGCGGAAGTC CACCTTTGAT 
GCAGCCAGCT TCATTGGAGG AATCGTCCTT GTCTTGGGTG TGCAGGCTGT AATTTTCTTT 
CTCTATAAAT TCTGCAAATC TAAAGAACGA AATTACCATA CTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
PLoS genetics11(7)e1005386(2015 Jul)
Nyegaard M,Rendtorff ND,Nielsen MS,Corydon TJ,Demontis D,Starnawska A,Hedemand A,Buniello A,Niola F,Overgaard MT,Leal SM,Ahmad W,Wikman FP,Petersen KB,Cr?ger DG,Oostrik J,Kremer H,Tommerup N,Fr?din M,Steel KP,Tranebj?rg L,B?rglum AD

Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.
Molecular biology of the cell23(18)3636-46(2012 Sep)
Mo D,Costa SA,Ihrke G,Youker RT,Pastor-Soler N,Hughey RP,Weisz OA

Specific N-glycans direct apical delivery of transmembrane, but not soluble or glycosylphosphatidylinositol-anchored forms of endolyn in Madin-Darby canine kidney cells.
Molecular biology of the cell15(3)1407-16(2004 Mar)
Potter BA,Ihrke G,Bruns JR,Weixel KM,Weisz OA

Relationship between novel isoforms, functionally important domains, and subcellular distribution of CD164/endolyn.
The Journal of biological chemistry276(3)2139-52(2001 Jan)
Chan JY,Lee-Prudhoe JE,Jorgensen B,Ihrke G,Doyonnas R,Zannettino AC,Buckle VJ,Ward CJ,Simmons PJ,Watt SM

Endolyn is a mucin-like type I membrane protein targeted to lysosomes by its cytoplasmic tail.
The Biochemical journal345 Pt 2287-96(2000 Jan)
Ihrke G,Gray SR,Luzio JP