Prkch cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkch
Entrez Gene ID	81749
Full Name	protein kinase C, eta
General protein information	Preferred Names protein kinase C, eta Names protein kinase C eta type PKC-L nPKC-eta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	catalyzes the phospholipid and TPA-dependent, calcium independent phosphorylation of several peptide substrates [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764720.2	XP_008762942.1	protein kinase C eta type isoform X1
NM_031085.2	NP_112347.1	protein kinase C eta type

Pathways from BioSystems

Homologies

	PRKCH	XP_002805131.1
Rattus norvegicus (Norway rat)	Prkch	NP_112347.1
Xenopus tropicalis (tropical clawed frog)	prkch	XP_002939521.2
Canis lupus familiaris (dog)	PRKCH	XP_547844.2
Gallus gallus (chicken)	PRKCH	XP_421417.3
Danio rerio (zebrafish)	prkcha	NP_001116528.1
Homo sapiens (human)	PRKCH	NP_006246.2
Bos taurus (cattle)	PRKCH	NP_001070331.1
Mus musculus (house mouse)	Prkch	NP_032882.2
Pan troglodytes (chimpanzee)	PRKCH	XP_001168420.1

Gene Ontology
Bibliography

Related articles in PubMed

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, W?lfing C, Malissen B, Malissen M
Nature immunology14(8)858-66(2013 Aug)

PKC? promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria.
Lau E, Kluger H, Varsano T, Lee K, Scheffler I, Rimm DL, Ideker T, Ronai ZA
Cell148(3)543-55(2012 Feb)

Protein kinase C? activates NF-?B in response to camptothecin-induced DNA damage.
Raveh-Amit H, Hai N, Rotem-Dai N, Shahaf G, Gopas J, Livneh E
Biochemical and biophysical research communications412(2)313-7(2011 Aug)

Direct binding of RalA to PKC? and its crucial role in morphological change during keratinocyte differentiation.
Shirai Y, Morioka S, Sakuma M, Yoshino K, Otsuji C, Sakai N, Kashiwagi K, Chida K, Shirakawa R, Horiuchi H, Nishigori C, Ueyama T, Saito N
Molecular biology of the cell22(8)1340-52(2011 Apr)

Protein kinase C-eta and phospholipase D2 pathway regulates foam cell formation via regulator of G protein signaling 2.
Lee HK, Yeo S, Kim JS, Lee JG, Bae YS, Lee C, Baek SH
Molecular pharmacology78(3)478-85(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DGK interacts selectively with and is phosphorylated by PKCepsilon and -eta and peptide agonist-induced selective activation of PKCepsilon directly leads to DGK translocation
Title: Translocation of diacylglycerol kinase theta from cytosol to plasma membrane in response to activation of G protein-coupled receptors and protein kinase C.

both epidermal growth factor and thyrotropin releasing hormone stimulated prolactin gene transcription appear to require protein kinase C delta, whereas protein kinase C eta may also be able to transmit the epidermal growth factor response
Title: Role of specific protein kinase C isozymes in mediating epidermal growth factor, thyrotropin-releasing hormone, and phorbol ester regulation of the rat prolactin promoter in GH4/GH4C1 pituitary cells.

The following Prkch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07551	NM_031085.2 Latest version!	Rattus norvegicus protein kinase C, eta (Prkch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$405.30 $579.00
ORa43730	XM_008764720.2 Latest version!	Rattus norvegicus protein kinase C, eta (Prkch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkch ( NM_031085.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112347.1 Rattus norvegicus protein kinase C, eta (Prkch), mRNA.

Price & Availability↑

CloneID	ORa07551
Clone ID Related Accession (Same CDS sequence)	NM_031085.2
Accession Version	NM_031085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C eta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081782.1. On Sep 16, 2004 this sequence version replaced NM_031085.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081782.1, X68400.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTCCG GCACGATGAA GTTCAATGGC TATCTGAGGG TCCGCATCGG AGAGGCAGTG 
GGGCTGCAGC CCACCCGCTG GTCCCTGCGG CACTCGCTCT TCAAAAAGGG CCACCAGCTG 
CTGGACCCCT ACCTGACGGT GAGCGTAGAC CAGGTACGCG TGGGCCAGAC CAGCACAAAG 
CAGAAGACTA ACAAACCCAC CTACAACGAG GAGTTCTGCA CCAATGTCTC CGACGGCGGC 
CACCTGGAGC TAGCCGTCTT CCACGAGACG CCCCTGGGCT ATGACCACTT TGTGGCCAAC 
TGCACGCTGC AGTTCCAGGA GCTGTTGCGC ACGGCCGGCA CATCGGACAC CTTCGAGGGC 
TGGGTGGATC TGGAGCCTGA GGGGAAAGTG TTCGTGGTAA TAACCCTAAC AGGGAGCTTC 
ACTGAAGCCA CTCTCCAGAG AGACCGCATC TTCAAGCATT TTACCAGGAA GCGCCAACGG 
GCTATGCGAA GGAGAGTCCA TCAAGTCAAC GGACACAAGT TCATGGCCAC ATACCTGAGG 
CAGCCCACCT ACTGCTCTCA CTGCCGGGAG TTCATCTGGG GAGTATTTGG GAAACAGGGT 
TATCAATGCC AAGTGTGCAC CTGCGTCGTC CATAAACGCT GCCATCACCT AATTGTTACA 
GCCTGCACTT GCCAAAACAA TATTAACAAA GTGGATGCCA AGATCGCAGA GCAACGGTTT 
GGCATCAACA TCCCACACAA GTTCAACGTT CACAACTACA AGGTGCCCAC GTTCTGCGAC 
CACTGTGGCT CCCTGCTCTG GGGGATAATG CGACAAGGAC TTCAGTGTAA AATATGTAAG 
ATGAACGTAC ATATTCGGTG TCAGGCGAAC GTGGCCCCAA ACTGCGGGGT GAACGCCGTG 
GAGCTTGCCA AGACCCTGGC AGGGATGGGT CTCCAACCCG GAAATATTTC TCCAACCTCG 
AAACTCATTT CCAGGTCTAC GCTGAGACGG CAGGGGAAGG AGGGCTCCAA AGAAGGAAAT 
GGGATCGGTG TTAATTCTTC CAGCAGATTC GGCATCGACA ACTTTGAGTT CATCCGGGTG 
TTGGGGAAGG GGAGCTTCGG GAAGGTGATG CTCGCCAGAA TAAAGGAGAC AGGAGAGCTG 
TACGCTGTGA AGGTGCTGAA GAAGGACGTC ATCCTGCAGG ATGACGATGT GGAATGCACC 
ATGACTGAGA AGAGGATCCT CTCCTTGGCC CGCAACCACC CCTTCCTCAC CCAGCTCTTC 
TGCTGCTTTC AGACTCCTGA CCGTCTGTTC TTTGTCATGG AGTTTGTGAA CGGAGGTGAC 
CTGATGTTCC ACATCCAAAA GTCCAGGCGT TTCGATGAAG CCCGAGCTCG TTTCTACGCT 
GCAGAGATCA TTTCTGCACT CATGTTCCTC CACGAGAAAG GCATCATCTA CAGAGACTTG 
AAACTGGACA ACGTACTACT GGACCACGAA GGCCACTGTA AACTGGCCGA CTTCGGAATG 
TGCAAGGAGG GCATTTGTAA CGGGGTCACC ACAGCCACCT TCTGCGGCAC GCCTGACTAC 
ATTGCCCCAG AGATCCTTCA GGAGATGTTG TATGGACCTG CAGTAGACTG GTGGGCCATG 
GGCGTGTTGC TTTATGAGAT GCTGTGCGGA CATGCGCCCT TCGAGGCTGA GAATGAAGAT 
GACCTTTTTG AGGCCATACT GAATGATGAA GTTGTCTACC CTACCTGGCT CCATGAAGAT 
GCCACAGGGA TCCTCAAATC TTTCATGACC AAGAACCCCA CCATGCGCTT GGGCAGCCTG 
ACTCAGGGAG GCGAGCATGA GATCTTGAGA CATCCTTTCT TTAAGGAAAT CGACTGGGTC 
CAGTTGAACC ATCGCCAGCT AGAACCGCCT TTCCGACCCA GAATCAAATC CCGAGAAGAC 
GTCAGCAATT TTGACCCAGA CTTCATAAAA GAAGAGCCTG TTTTAACTCC GATCGATGAG 
GGACATCTTC CTATGATTAA CCAGGATGAG TTTAGAAACT TTTCCTATGT GTCACCAGAA 
TTGCAACCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112347.1
CDS	188..2239
Translation	MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNEEFCTNVSDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTAGTSDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQVNGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDAKIAEQRFGINIPHKFNVHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLISRSTLRRQGKEGSKEGNGIGVNSSSRFGIDNFEFIRVLGKGSFGKVMLARIKETGELYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHEKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHEILRHPFFKEIDWVQLNHRQLEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP

Target ORF information:

RefSeq Version	NM_031085.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, eta (Prkch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031085.2

ORF Insert Sequence:

ATGTCGTCCG GCACGATGAA GTTCAATGGC TATCTGAGGG TCCGCATCGG AGAGGCAGTG 
GGGCTGCAGC CCACCCGCTG GTCCCTGCGG CACTCGCTCT TCAAAAAGGG CCACCAGCTG 
CTGGACCCCT ACCTGACGGT GAGCGTAGAC CAGGTACGCG TGGGCCAGAC CAGCACAAAG 
CAGAAGACTA ACAAACCCAC CTACAACGAG GAGTTCTGCA CCAATGTCTC CGACGGCGGC 
CACCTGGAGC TAGCCGTCTT CCACGAGACG CCCCTGGGCT ATGACCACTT TGTGGCCAAC 
TGCACGCTGC AGTTCCAGGA GCTGTTGCGC ACGGCCGGCA CATCGGACAC CTTCGAGGGC 
TGGGTGGATC TGGAGCCTGA GGGGAAAGTG TTCGTGGTAA TAACCCTAAC AGGGAGCTTC 
ACTGAAGCCA CTCTCCAGAG AGACCGCATC TTCAAGCATT TTACCAGGAA GCGCCAACGG 
GCTATGCGAA GGAGAGTCCA TCAAGTCAAC GGACACAAGT TCATGGCCAC ATACCTGAGG 
CAGCCCACCT ACTGCTCTCA CTGCCGGGAG TTCATCTGGG GAGTATTTGG GAAACAGGGT 
TATCAATGCC AAGTGTGCAC CTGCGTCGTC CATAAACGCT GCCATCACCT AATTGTTACA 
GCCTGCACTT GCCAAAACAA TATTAACAAA GTGGATGCCA AGATCGCAGA GCAACGGTTT 
GGCATCAACA TCCCACACAA GTTCAACGTT CACAACTACA AGGTGCCCAC GTTCTGCGAC 
CACTGTGGCT CCCTGCTCTG GGGGATAATG CGACAAGGAC TTCAGTGTAA AATATGTAAG 
ATGAACGTAC ATATTCGGTG TCAGGCGAAC GTGGCCCCAA ACTGCGGGGT GAACGCCGTG 
GAGCTTGCCA AGACCCTGGC AGGGATGGGT CTCCAACCCG GAAATATTTC TCCAACCTCG 
AAACTCATTT CCAGGTCTAC GCTGAGACGG CAGGGGAAGG AGGGCTCCAA AGAAGGAAAT 
GGGATCGGTG TTAATTCTTC CAGCAGATTC GGCATCGACA ACTTTGAGTT CATCCGGGTG 
TTGGGGAAGG GGAGCTTCGG GAAGGTGATG CTCGCCAGAA TAAAGGAGAC AGGAGAGCTG 
TACGCTGTGA AGGTGCTGAA GAAGGACGTC ATCCTGCAGG ATGACGATGT GGAATGCACC 
ATGACTGAGA AGAGGATCCT CTCCTTGGCC CGCAACCACC CCTTCCTCAC CCAGCTCTTC 
TGCTGCTTTC AGACTCCTGA CCGTCTGTTC TTTGTCATGG AGTTTGTGAA CGGAGGTGAC 
CTGATGTTCC ACATCCAAAA GTCCAGGCGT TTCGATGAAG CCCGAGCTCG TTTCTACGCT 
GCAGAGATCA TTTCTGCACT CATGTTCCTC CACGAGAAAG GCATCATCTA CAGAGACTTG 
AAACTGGACA ACGTACTACT GGACCACGAA GGCCACTGTA AACTGGCCGA CTTCGGAATG 
TGCAAGGAGG GCATTTGTAA CGGGGTCACC ACAGCCACCT TCTGCGGCAC GCCTGACTAC 
ATTGCCCCAG AGATCCTTCA GGAGATGTTG TATGGACCTG CAGTAGACTG GTGGGCCATG 
GGCGTGTTGC TTTATGAGAT GCTGTGCGGA CATGCGCCCT TCGAGGCTGA GAATGAAGAT 
GACCTTTTTG AGGCCATACT GAATGATGAA GTTGTCTACC CTACCTGGCT CCATGAAGAT 
GCCACAGGGA TCCTCAAATC TTTCATGACC AAGAACCCCA CCATGCGCTT GGGCAGCCTG 
ACTCAGGGAG GCGAGCATGA GATCTTGAGA CATCCTTTCT TTAAGGAAAT CGACTGGGTC 
CAGTTGAACC ATCGCCAGCT AGAACCGCCT TTCCGACCCA GAATCAAATC CCGAGAAGAC 
GTCAGCAATT TTGACCCAGA CTTCATAAAA GAAGAGCCTG TTTTAACTCC GATCGATGAG 
GGACATCTTC CTATGATTAA CCAGGATGAG TTTAGAAACT TTTCCTATGT GTCACCAGAA 
TTGCAACCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkch ( XM_008764720.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762942.1 Rattus norvegicus protein kinase C, eta (Prkch), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43730
Clone ID Related Accession (Same CDS sequence)	XM_008764720.2
Accession Version	XM_008764720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C eta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTTT CAGAGGTGGA TCTGGAGCCT GAGGGGAAAG TGTTCGTGGT AATAACCCTA 
ACAGGGAGCT TCACTGAAGC CACTCTCCAG AGAGACCGCA TCTTCAAGCA TTTTACCAGG 
AAGCGCCAAC GGGCTATGCG AAGGAGAGTC CATCAAGTCA ACGGACACAA GTTCATGGCC 
ACATACCTGA GGCAGCCCAC CTACTGCTCT CACTGCCGGG AGTTCATCTG GGGAGTATTT 
GGGAAACAGG GTTATCAATG CCAAGTGTGC ACCTGCGTCG TCCATAAACG CTGCCATCAC 
CTAATTGTTA CAGCCTGCAC TTGCCAAAAC AATATTAACA AAGTGGATGC CAAGATCGCA 
GAGCAACGGT TTGGCATCAA CATCCCACAC AAGTTCAACG TTCACAACTA CAAGGTGCCC 
ACGTTCTGCG ACCACTGTGG CTCCCTGCTC TGGGGGATAA TGCGACAAGG ACTTCAGTGT 
AAAATATGTA AGATGAACGT ACATATTCGG TGTCAGGCGA ACGTGGCCCC AAACTGCGGG 
GTGAACGCCG TGGAGCTTGC CAAGACCCTG GCAGGGATGG GTCTCCAACC CGGAAATATT 
TCTCCAACCT CGAAACTCAT TTCCAGGTCT ACGCTGAGAC GGCAGGGGAA GGAGGGCTCC 
AAAGAAGGAA ATGGGATCGG TGTTAATTCT TCCAGCAGAT TCGGCATCGA CAACTTTGAG 
TTCATCCGGG TGTTGGGGAA GGGGAGCTTC GGGAAGGTGA TGCTCGCCAG AATAAAGGAG 
ACAGGAGAGC TGTACGCTGT GAAGGTGCTG AAGAAGGACG TCATCCTGCA GGATGACGAT 
GTGGAATGCA CCATGACTGA GAAGAGGATC CTCTCCTTGG CCCGCAACCA CCCCTTCCTC 
ACCCAGCTCT TCTGCTGCTT TCAGACTCCT GACCGTCTGT TCTTTGTCAT GGAGTTTGTG 
AACGGAGGTG ACCTGATGTT CCACATCCAA AAGTCCAGGC GTTTCGATGA AGCCCGAGCT 
CGTTTCTACG CTGCAGAGAT CATTTCTGCA CTCATGTTCC TCCACGAGAA AGGCATCATC 
TACAGAGACT TGAAACTGGA CAACGTACTA CTGGACCACG AAGGCCACTG TAAACTGGCC 
GACTTCGGAA TGTGCAAGGA GGGCATTTGT AACGGGGTCA CCACAGCCAC CTTCTGCGGC 
ACGCCTGACT ACATTGCCCC AGAGATCCTT CAGGAGATGT TGTATGGACC TGCAGTAGAC 
TGGTGGGCCA TGGGCGTGTT GCTTTATGAG ATGCTGTGCG GACATGCGCC CTTCGAGGCT 
GAGAATGAAG ATGACCTTTT TGAGGCCATA CTGAATGATG AAGTTGTCTA CCCTACCTGG 
CTCCATGAAG ATGCCACAGG GATCCTCAAA TCTTTCATGA CCAAGAACCC CACCATGCGC 
TTGGGCAGCC TGACTCAGGG AGGCGAGCAT GAGATCTTGA GACATCCTTT CTTTAAGGAA 
ATCGACTGGG TCCAGTTGAA CCATCGCCAG CTAGAACCGC CTTTCCGACC CAGAATCAAA 
TCCCGAGAAG ACGTCAGCAA TTTTGACCCA GACTTCATAA AAGAAGAGCC TGTTTTAACT 
CCGATCGATG AGGGACATCT TCCTATGATT AACCAGGATG AGTTTAGAAA CTTTTCCTAT 
GTGTCACCAG AATTGCAACC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762942.1
CDS	2598..4301
Translation	MDVSEVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQVNGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDAKIAEQRFGINIPHKFNVHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLISRSTLRRQGKEGSKEGNGIGVNSSSRFGIDNFEFIRVLGKGSFGKVMLARIKETGELYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHEKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHEILRHPFFKEIDWVQLNHRQLEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP

Target ORF information:

RefSeq Version	XM_008764720.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, eta (Prkch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764720.2

ORF Insert Sequence:

ATGGATGTTT CAGAGGTGGA TCTGGAGCCT GAGGGGAAAG TGTTCGTGGT AATAACCCTA 
ACAGGGAGCT TCACTGAAGC CACTCTCCAG AGAGACCGCA TCTTCAAGCA TTTTACCAGG 
AAGCGCCAAC GGGCTATGCG AAGGAGAGTC CATCAAGTCA ACGGACACAA GTTCATGGCC 
ACATACCTGA GGCAGCCCAC CTACTGCTCT CACTGCCGGG AGTTCATCTG GGGAGTATTT 
GGGAAACAGG GTTATCAATG CCAAGTGTGC ACCTGCGTCG TCCATAAACG CTGCCATCAC 
CTAATTGTTA CAGCCTGCAC TTGCCAAAAC AATATTAACA AAGTGGATGC CAAGATCGCA 
GAGCAACGGT TTGGCATCAA CATCCCACAC AAGTTCAACG TTCACAACTA CAAGGTGCCC 
ACGTTCTGCG ACCACTGTGG CTCCCTGCTC TGGGGGATAA TGCGACAAGG ACTTCAGTGT 
AAAATATGTA AGATGAACGT ACATATTCGG TGTCAGGCGA ACGTGGCCCC AAACTGCGGG 
GTGAACGCCG TGGAGCTTGC CAAGACCCTG GCAGGGATGG GTCTCCAACC CGGAAATATT 
TCTCCAACCT CGAAACTCAT TTCCAGGTCT ACGCTGAGAC GGCAGGGGAA GGAGGGCTCC 
AAAGAAGGAA ATGGGATCGG TGTTAATTCT TCCAGCAGAT TCGGCATCGA CAACTTTGAG 
TTCATCCGGG TGTTGGGGAA GGGGAGCTTC GGGAAGGTGA TGCTCGCCAG AATAAAGGAG 
ACAGGAGAGC TGTACGCTGT GAAGGTGCTG AAGAAGGACG TCATCCTGCA GGATGACGAT 
GTGGAATGCA CCATGACTGA GAAGAGGATC CTCTCCTTGG CCCGCAACCA CCCCTTCCTC 
ACCCAGCTCT TCTGCTGCTT TCAGACTCCT GACCGTCTGT TCTTTGTCAT GGAGTTTGTG 
AACGGAGGTG ACCTGATGTT CCACATCCAA AAGTCCAGGC GTTTCGATGA AGCCCGAGCT 
CGTTTCTACG CTGCAGAGAT CATTTCTGCA CTCATGTTCC TCCACGAGAA AGGCATCATC 
TACAGAGACT TGAAACTGGA CAACGTACTA CTGGACCACG AAGGCCACTG TAAACTGGCC 
GACTTCGGAA TGTGCAAGGA GGGCATTTGT AACGGGGTCA CCACAGCCAC CTTCTGCGGC 
ACGCCTGACT ACATTGCCCC AGAGATCCTT CAGGAGATGT TGTATGGACC TGCAGTAGAC 
TGGTGGGCCA TGGGCGTGTT GCTTTATGAG ATGCTGTGCG GACATGCGCC CTTCGAGGCT 
GAGAATGAAG ATGACCTTTT TGAGGCCATA CTGAATGATG AAGTTGTCTA CCCTACCTGG 
CTCCATGAAG ATGCCACAGG GATCCTCAAA TCTTTCATGA CCAAGAACCC CACCATGCGC 
TTGGGCAGCC TGACTCAGGG AGGCGAGCAT GAGATCTTGA GACATCCTTT CTTTAAGGAA 
ATCGACTGGG TCCAGTTGAA CCATCGCCAG CTAGAACCGC CTTTCCGACC CAGAATCAAA 
TCCCGAGAAG ACGTCAGCAA TTTTGACCCA GACTTCATAA AAGAAGAGCC TGTTTTAACT 
CCGATCGATG AGGGACATCT TCCTATGATT AACCAGGATG AGTTTAGAAA CTTTTCCTAT 
GTGTCACCAG AATTGCAACC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Nature immunology14(8)858-66(2013 Aug)
Liang Y,Cucchetti M,Roncagalli R,Yokosuka T,Malzac A,Bertosio E,Imbert J,Nijman IJ,Suchanek M,Saito T,W?lfing C,Malissen B,Malissen M

PKC? promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria.
Cell148(3)543-55(2012 Feb)
Lau E,Kluger H,Varsano T,Lee K,Scheffler I,Rimm DL,Ideker T,Ronai ZA

Protein kinase C? activates NF-?B in response to camptothecin-induced DNA damage.
Biochemical and biophysical research communications412(2)313-7(2011 Aug)
Raveh-Amit H,Hai N,Rotem-Dai N,Shahaf G,Gopas J,Livneh E

Direct binding of RalA to PKC? and its crucial role in morphological change during keratinocyte differentiation.
Molecular biology of the cell22(8)1340-52(2011 Apr)
Shirai Y,Morioka S,Sakuma M,Yoshino K,Otsuji C,Sakai N,Kashiwagi K,Chida K,Shirakawa R,Horiuchi H,Nishigori C,Ueyama T,Saito N

Protein kinase C-eta and phospholipase D2 pathway regulates foam cell formation via regulator of G protein signaling 2.
Molecular pharmacology78(3)478-85(2010 Sep)
Lee HK,Yeo S,Kim JS,Lee JG,Bae YS,Lee C,Baek SH