Lbh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lbh
Entrez Gene ID	683626
Full Name	limb bud and heart development
General protein information	Preferred Names limb bud and heart development Names protein LBH
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764533.1	XP_008762755.1	protein LBH isoform X1
NM_001129880.2	NP_001123352.1	protein LBH

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:66337	NP_956814.1
Xenopus tropicalis (tropical clawed frog)	lbh	NP_001011389.1
Canis lupus familiaris (dog)	LBH	XP_853968.1
	LBH	XP_001104344.1
Rattus norvegicus (Norway rat)	Lbh	NP_001123352.1
Gallus gallus (chicken)	LBH	NP_001026209.1
Pan troglodytes (chimpanzee)	LBH	XP_001163096.1
Mus musculus (house mouse)	Lbh	NP_084275.3
Homo sapiens (human)	LBH	NP_112177.2
Bos taurus (cattle)	LBH	NP_001092622.1

Gene Ontology
Bibliography

Related articles in PubMed

The WNT-controlled transcriptional regulator LBH is required for mammary stem cell expansion and maintenance of the basal lineage.
Lindley LE, Curtis KM, Sanchez-Mejias A, Rieger ME, Robbins DJ, Briegel KJ
Development (Cambridge, England)142(5)893-904(2015 Mar)

Combined analysis of three genome-wide association studies on vWF and FVIII plasma levels.
Antoni G, Oudot-Mellakh T, Dimitromanolakis A, Germain M, Cohen W, Wells P, Lathrop M, Gagnon F, Morange PE, Tregouet DA
BMC medical genetics12102(2011 Aug)

Synergistic efficacy of LBH and alphaB-crystallin through inhibiting transcriptional activities of p53 and p21.
Deng Y, Li Y, Fan X, Yuan W, Xie H, Mo X, Yan Y, Zhou J, Wang Y, Ye X, Wan Y, Wu X
BMB reports43(6)432-7(2010 Jun)

A human homolog of mouse Lbh gene, hLBH, expresses in heart and activates SRE and AP-1 mediated MAPK signaling pathway.
Ai J, Wang Y, Tan K, Deng Y, Luo N, Yuan W, Wang Z, Li Y, Wang Y, Mo X, Zhu C, Yin Z, Liu M, Wu X
Molecular biology reports35(2)179-87(2008 Jun)

Identification and characterization of Lbh, a novel conserved nuclear protein expressed during early limb and heart development.
Briegel KJ, Joyner AL
Developmental biology233(2)291-304(2001 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lbh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lbh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02226	NM_001129880.2 Latest version!	Rattus norvegicus LBH regulator of WNT signaling pathway (Lbh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa49957	XM_008764533.1 Latest version!	Rattus norvegicus limb bud and heart development (Lbh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lbh ( NM_001129880.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001123352.1 Rattus norvegicus LBH regulator of WNT signaling pathway (Lbh), mRNA.

Price & Availability↑

CloneID	ORa02226
Clone ID Related Accession (Same CDS sequence)	NM_001129880.2
Accession Version	NM_001129880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-06
Organism	Rattus norvegicus(Norway rat)
Product	protein LBH
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB744030.1, BC166725.1, BE100104.1, CO559287.1, DN930991.1 and EX491960.1. On Sep 13, 2008 this sequence version replaced NM_001129880.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGTAT ATTTCCCCAT TCACTGCTCT GACTATCTGA GATCGGCTGA GATGACTGAG 
GTGATGATGA ATGCTCCCTC CATGGAAGAG ATTGGCCTCA GCCCCCGCAA GGATGGCCTT 
TCCTATCAGA TCTTTCCCGA CCCATCAGAT TTTGACCGCT GTTGCAAACT GAAGGACCGC 
CTGCCCTCCA TAGTGGTGGA ACCCACAGAA GGGGAGGTGG AGAGTGGGGA GCTCCGGTGG 
CCCCCTGAGG AATTCCTGGT CCAGGAGGAT GAGCAGGACA ACTGTGAAGA GACAACAAAA 
GAAAAGAAGG ATCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123352.1
CDS	221..538
Translation	MSVYFPIHCSDYLRSAEMTEVMMNAPSMEEIGLSPRKDGLSYQIFPDPSDFDRCCKLKDRLPSIVVEPTEGEVESGELRWPPEEFLVQEDEQDNCEETTKEKKDQ

Target ORF information:

RefSeq Version	NM_001129880.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LBH regulator of WNT signaling pathway (Lbh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129880.2

ORF Insert Sequence:

ATGTCTGTAT ATTTCCCCAT TCACTGCTCT GACTATCTGA GATCGGCTGA GATGACTGAG 
GTGATGATGA ATGCTCCCTC CATGGAAGAG ATTGGCCTCA GCCCCCGCAA GGATGGCCTT 
TCCTATCAGA TCTTTCCCGA CCCATCAGAT TTTGACCGCT GTTGCAAACT GAAGGACCGC 
CTGCCCTCCA TAGTGGTGGA ACCCACAGAA GGGGAGGTGG AGAGTGGGGA GCTCCGGTGG 
CCCCCTGAGG AATTCCTGGT CCAGGAGGAT GAGCAGGACA ACTGTGAAGA GACAACAAAA 
GAAAAGAAGG ATCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lbh ( XM_008764533.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762755.1 Rattus norvegicus limb bud and heart development (Lbh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa49957
Clone ID Related Accession (Same CDS sequence)	XM_008764533.1
Accession Version	XM_008764533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein LBH isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 47% of CDS bases ##RefSeq-Attributes-END##

ATGCCGCAGC CTTTAAAGTC CCAGGCTGTG CGCGGGAAGG GGGCTGCGGA CGCTGCAGAT 
TTGTGGGCAG GAAGCAGCAT TGACATTAAC TCCAGCTCGG AACGCCGCGT GGTGTCAACG 
AGTGGGCAAG GGAGGCGGGC GCCCCGGACA AGCCCCGCCC TCTCTTATAA ATGCTGCTTC 
CCGGGCTCTT TGTGGGACCC GGACTCGGTG GAGAGCGGAA GCTTGGTCTG CACTGCCGAC 
GCCGCTGGCA GCGAGGCTGT CTCCGGAAGG CACCTGCGGA TAACCAGAAT CAGTGACCGT 
TTTTTTTTTT TTTTTGTTGT TTTGTTTTGT TTCTTAATCA CAAGGGCGTG GTCAGCTCCC 
CTAGGACTTC ATGTCGTATA TTTCCCCATT CACTGCTCTG ACTATCTGAG ATCGGCTGAG 
ATGACTGAGG TGATGATGAA TGCTCCCTCC ATGGAAGAGA TTGGCCTCAG CCCCCGCAAG 
GATGGCCTTT CCTATCAGAT CTTTCCCGAC CCATCAGATT TTGACCGCTG TTGCAAACTG 
AAGGACCGCC TGCCCTCCAT AGTGGTGGAA CCCACAGAAG GGGAGGTGGA GAGTGGGGAG 
CTCCGGTGGC CCCCTGAGGA ATTCCTGGTC CAGGAGGATG AGCAGGACAA CTGTGAAGAG 
ACAACAAAAG AAAAGAAGGA TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762755.1
CDS	1..687
Translation	MPQPLKSQAVRGKGAADAADLWAGSSIDINSSSERRVVSTSGQGRRAPRTSPALSYKCCFPGSLWDPDSVESGSLVCTADAAGSEAVSGRHLRITRISDRFFFFFVVLFCFLITRAWSAPLGLHVVYFPIHCSDYLRSAEMTEVMMNAPSMEEIGLSPRKDGLSYQIFPDPSDFDRCCKLKDRLPSIVVEPTEGEVESGELRWPPEEFLVQEDEQDNCEETTKEKKDQ

Target ORF information:

RefSeq Version	XM_008764533.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus limb bud and heart development (Lbh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764533.1

ORF Insert Sequence:

ATGCCGCAGC CTTTAAAGTC CCAGGCTGTG CGCGGGAAGG GGGCTGCGGA CGCTGCAGAT 
TTGTGGGCAG GAAGCAGCAT TGACATTAAC TCCAGCTCGG AACGCCGCGT GGTGTCAACG 
AGTGGGCAAG GGAGGCGGGC GCCCCGGACA AGCCCCGCCC TCTCTTATAA ATGCTGCTTC 
CCGGGCTCTT TGTGGGACCC GGACTCGGTG GAGAGCGGAA GCTTGGTCTG CACTGCCGAC 
GCCGCTGGCA GCGAGGCTGT CTCCGGAAGG CACCTGCGGA TAACCAGAAT CAGTGACCGT 
TTTTTTTTTT TTTTTGTTGT TTTGTTTTGT TTCTTAATCA CAAGGGCGTG GTCAGCTCCC 
CTAGGACTTC ATGTCGTATA TTTCCCCATT CACTGCTCTG ACTATCTGAG ATCGGCTGAG 
ATGACTGAGG TGATGATGAA TGCTCCCTCC ATGGAAGAGA TTGGCCTCAG CCCCCGCAAG 
GATGGCCTTT CCTATCAGAT CTTTCCCGAC CCATCAGATT TTGACCGCTG TTGCAAACTG 
AAGGACCGCC TGCCCTCCAT AGTGGTGGAA CCCACAGAAG GGGAGGTGGA GAGTGGGGAG 
CTCCGGTGGC CCCCTGAGGA ATTCCTGGTC CAGGAGGATG AGCAGGACAA CTGTGAAGAG 
ACAACAAAAG AAAAGAAGGA TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The WNT-controlled transcriptional regulator LBH is required for mammary stem cell expansion and maintenance of the basal lineage.
Development (Cambridge, England)142(5)893-904(2015 Mar)
Lindley LE,Curtis KM,Sanchez-Mejias A,Rieger ME,Robbins DJ,Briegel KJ

Combined analysis of three genome-wide association studies on vWF and FVIII plasma levels.
BMC medical genetics12102(2011 Aug)
Antoni G,Oudot-Mellakh T,Dimitromanolakis A,Germain M,Cohen W,Wells P,Lathrop M,Gagnon F,Morange PE,Tregouet DA

Synergistic efficacy of LBH and alphaB-crystallin through inhibiting transcriptional activities of p53 and p21.
BMB reports43(6)432-7(2010 Jun)
Deng Y,Li Y,Fan X,Yuan W,Xie H,Mo X,Yan Y,Zhou J,Wang Y,Ye X,Wan Y,Wu X

A human homolog of mouse Lbh gene, hLBH, expresses in heart and activates SRE and AP-1 mediated MAPK signaling pathway.
Molecular biology reports35(2)179-87(2008 Jun)
Ai J,Wang Y,Tan K,Deng Y,Luo N,Yuan W,Wang Z,Li Y,Wang Y,Mo X,Zhu C,Yin Z,Liu M,Wu X

Identification and characterization of Lbh, a novel conserved nuclear protein expressed during early limb and heart development.
Developmental biology233(2)291-304(2001 May)
Briegel KJ,Joyner AL