Ech1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ech1
Entrez Gene ID	64526
Full Name	enoyl-CoA hydratase 1
Synonyms	Pxel
General protein information	Preferred Names enoyl-CoA hydratase 1 Names delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Peroxisomal enoyl hydratase-like protein enoyl CoA hydratase 1, peroxisomal enoyl coenzyme A hydratase 1, peroxisomal enoyl hydratase-like protein, peroxisomal
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	isomerase enzyme that may be involved with the ability of peroxisomes to oxidize long-chain faty acids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022594.1	NP_072116.1	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ech1	NP_058052.1
Magnaporthe oryzae (rice blast fungus)	MGG_07309	XP_003715542.1
Xenopus tropicalis (tropical clawed frog)	ech1	NP_001005704.1
Pan troglodytes (chimpanzee)	ECH1	XP_003316368.1
Bos taurus (cattle)	ECH1	XP_002695024.1
Neurospora crassa	NCU06647	XP_960880.1
Homo sapiens (human)	ECH1	NP_001389.2
Caenorhabditis elegans (roundworm)	Y25C1A.13	NP_494448.1
	ECH1	XP_001084167.2
Canis lupus familiaris (dog)	ECH1	XP_533678.2
Danio rerio (zebrafish)	ech1	NP_001006068.1
Caenorhabditis elegans (roundworm)	F58A6.1	NP_494954.1
Arabidopsis thaliana (thale cress)	DCI1	NP_199142.1
Oryza sativa (rice)	Os01g0925200	NP_001045254.1
Rattus norvegicus (Norway rat)	Ech1	NP_072116.1
Gallus gallus (chicken)	ECH1	XP_003643886.1
Drosophila melanogaster (fruit fly)	CG9577	NP_608375.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002661	XP_312264.5

Gene Ontology
Bibliography

Related articles in PubMed

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

The crystal structure of dienoyl-CoA isomerase at 1.5 A resolution reveals the importance of aspartate and glutamate sidechains for catalysis.
Modis Y, Filppula SA, Novikov DK, Norledge B, Hiltunen JK, Wierenga RK
Structure (London, England : 1993)6(8)957-70(1998 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ech1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ech1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06078	NM_022594.1 Latest version!	Rattus norvegicus enoyl-CoA hydratase 1 (Ech1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ech1 ( NM_022594.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072116.1 Rattus norvegicus enoyl-CoA hydratase 1 (Ech1), mRNA.

Price & Availability↑

CloneID	ORa06078
Clone ID Related Accession (Same CDS sequence)	NM_022594.1
Accession Version	NM_022594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U08976.1. ##Evidence-Data-START## Transcript exon combination :: U08976.1, BC060565.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACCG CGATGACAGT TTCCAGCAAA CTCCTCGGCC TGCTGATGCA GCAGCTGAGA 
GGTACCCGCC AGCTGTACTT CAACGTCAGC CTTCGATCTC TGAGCTCCTC TGCACAAGAG 
GCGTCCAAAA GAATCCCTGA GGAAGTCTCA GACCACAACT ACGAGTCTAT TCAAGTAACA 
TCTGCCCAGA AGCATGTTCT GCATGTACAA CTAAACCGGC CGGAGAAGAG GAATGCCATG 
AACAGGGCTT TCTGGAGGGA GTTGGTGGAA TGCTTCCAAA AGATATCTAA AGACTCCGAC 
TGTCGGGCTG TTGTGGTCTC TGGTGCAGGA AAGATGTTCA CTTCAGGTAT TGACCTCATG 
GACATGGCAT CAGACATCCT GCAGCCCCCA GGAGACGATG TGGCCCGCAT AGCCTGGTAC 
CTCCGAGACC TCATCAGCCG ATACCAAAAG ACTTTCACTG TCATTGAGAA GTGCCCCAAG 
CCGGTGATCA CCGCAATTCA CGGAGGCTGC ATTGGTGGAG GCGTGGATCT TATTTCTGCC 
TGTGACATTC GCTACTGCAC TCAGGATGCT TTCTTCCAAG TCAAGGAGGT GGATGTGGGC 
CTGGCTGCTG ATGTAGGAAC GCTGCAGCGA CTGCCCAAGG TCATTGGGAA CCGGAGCCTG 
GTCAACGAGC TGACCTTCAC CGCCCGCAAG ATGATGGCTG ACGAGGCCCT GGACAGTGGG 
CTGGTCAGCC GCGTGTTCCC AGATAAGGAC GTCATGCTGA ATGCAGCCTT TGCCCTGGCG 
GCCGATATTT CCAGCAAGAG TCCTGTGGCT GTGCAGGGGT CAAAAATCAA TTTAATCTAC 
TCCCGAGACC ATTCTGTGGA CGAGAGCCTT GACTATATGG CCACTTGGAA CATGAGCATG 
CTGCAGACCC AGGACATCAT CAAGTCAGTC CAGGCAGCCA TGGAGAAGAA GGACTCAAAA 
AGCATCACCT TCTCCAAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072116.1
CDS	11..994
Translation	MATAMTVSSKLLGLLMQQLRGTRQLYFNVSLRSLSSSAQEASKRIPEEVSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSGAGKMFTSGIDLMDMASDILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKPVITAIHGGCIGGGVDLISACDIRYCTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLVNELTFTARKMMADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL

Target ORF information:

RefSeq Version	NM_022594.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus enoyl-CoA hydratase 1 (Ech1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022594.1

ORF Insert Sequence:

ATGGCTACCG CGATGACAGT TTCCAGCAAA CTCCTCGGCC TGCTGATGCA GCAGCTGAGA 
GGTACCCGCC AGCTGTACTT CAACGTCAGC CTTCGATCTC TGAGCTCCTC TGCACAAGAG 
GCGTCCAAAA GAATCCCTGA GGAAGTCTCA GACCACAACT ACGAGTCTAT TCAAGTAACA 
TCTGCCCAGA AGCATGTTCT GCATGTACAA CTAAACCGGC CGGAGAAGAG GAATGCCATG 
AACAGGGCTT TCTGGAGGGA GTTGGTGGAA TGCTTCCAAA AGATATCTAA AGACTCCGAC 
TGTCGGGCTG TTGTGGTCTC TGGTGCAGGA AAGATGTTCA CTTCAGGTAT TGACCTCATG 
GACATGGCAT CAGACATCCT GCAGCCCCCA GGAGACGATG TGGCCCGCAT AGCCTGGTAC 
CTCCGAGACC TCATCAGCCG ATACCAAAAG ACTTTCACTG TCATTGAGAA GTGCCCCAAG 
CCGGTGATCA CCGCAATTCA CGGAGGCTGC ATTGGTGGAG GCGTGGATCT TATTTCTGCC 
TGTGACATTC GCTACTGCAC TCAGGATGCT TTCTTCCAAG TCAAGGAGGT GGATGTGGGC 
CTGGCTGCTG ATGTAGGAAC GCTGCAGCGA CTGCCCAAGG TCATTGGGAA CCGGAGCCTG 
GTCAACGAGC TGACCTTCAC CGCCCGCAAG ATGATGGCTG ACGAGGCCCT GGACAGTGGG 
CTGGTCAGCC GCGTGTTCCC AGATAAGGAC GTCATGCTGA ATGCAGCCTT TGCCCTGGCG 
GCCGATATTT CCAGCAAGAG TCCTGTGGCT GTGCAGGGGT CAAAAATCAA TTTAATCTAC 
TCCCGAGACC ATTCTGTGGA CGAGAGCCTT GACTATATGG CCACTTGGAA CATGAGCATG 
CTGCAGACCC AGGACATCAT CAAGTCAGTC CAGGCAGCCA TGGAGAAGAA GGACTCAAAA 
AGCATCACCT TCTCCAAGCT CTGA