Ech1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ech1
Entrez Gene ID	51798
Full Name	enoyl coenzyme A hydratase 1, peroxisomal
Synonyms	AA617331
General protein information	Preferred Names enoyl coenzyme A hydratase 1, peroxisomal Names delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial dienoyl-CoA isomerase peroxisomal/mitochondrial dienoyl-CoA isomerase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_016772.1	NP_058052.1	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ech1	NP_058052.1
Magnaporthe oryzae (rice blast fungus)	MGG_07309	XP_003715542.1
Xenopus tropicalis (tropical clawed frog)	ech1	NP_001005704.1
Pan troglodytes (chimpanzee)	ECH1	XP_003316368.1
Bos taurus (cattle)	ECH1	XP_002695024.1
Neurospora crassa	NCU06647	XP_960880.1
Homo sapiens (human)	ECH1	NP_001389.2
Caenorhabditis elegans (roundworm)	Y25C1A.13	NP_494448.1
	ECH1	XP_001084167.2
Canis lupus familiaris (dog)	ECH1	XP_533678.2
Danio rerio (zebrafish)	ech1	NP_001006068.1
Caenorhabditis elegans (roundworm)	F58A6.1	NP_494954.1
Arabidopsis thaliana (thale cress)	DCI1	NP_199142.1
Oryza sativa (rice)	Os01g0925200	NP_001045254.1
Rattus norvegicus (Norway rat)	Ech1	NP_072116.1
Gallus gallus (chicken)	ECH1	XP_003643886.1
Drosophila melanogaster (fruit fly)	CG9577	NP_608375.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002661	XP_312264.5

Gene Ontology
Bibliography

Related articles in PubMed

An Ideal PPAR Response Element Bound to and Activated by PPAR?.
Tzeng J, Byun J, Park JY, Yamamoto T, Schesing K, Tian B, Sadoshima J, Oka S
PloS one10(8)e0134996(2015)

Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.
Zhang J, Sun M, Li R, Liu S, Mao J, Huang Y, Wang B, Hou L, Ibrahim MM, Tang J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie67(7)557-60(2013 Sep)

[Down-regulation of Ech1 decreases the adhesion ability of mouse hepatocarcinoma Hca-F cells].
Zhang J, Tang JW, Sun MZ, Liu SQ, Song MY, Wang B, Song B, Huang YH
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology20(8)605-10(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

current work indicates the involvement of Ech1 in tumor metastasis development and progression, but the subcellular location of Ech1 has not much contribution to that.
Title: Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.

Down-regulation of Ech1 can inhibit the adhesive capacity of metastatic Hca-F cells.
Title: [Down-regulation of Ech1 decreases the adhesion ability of mouse hepatocarcinoma Hca-F cells].

Down-regulation of ECH1 could inhibit the cell proliferation and migration of Hca-F hepatocarcinoma cells.
Title: [Expression of enoyl CoA hydratase 1 reduces cell proliferation and migration in mouse hepatocarcinoma cells].

The downregulation of Ech1 inhibited proliferation of the Hca-F cells, increased the ratio of Hca-F cells in S phase to G(1) phase and decreased the adhesion and migration capacities of Hca-F cells.
Title: Enoyl coenzyme A hydratase 1 is an important factor in the lymphatic metastasis of tumors.

disruption of peroxisomal fatty acid beta-oxidation at the level of enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L-PBE) in mice leads to the induction of many of the PPARalpha target genes independently of peroxisome proliferation in hepatocytes
Title: Overexpression of peroxisome proliferator-activated receptor-alpha (PPARalpha)-regulated genes in liver in the absence of peroxisome proliferation in mice deficient in both L- and D-forms of enoyl-CoA hydratase/dehydrogenase enzymes of peroxisomal beta-oxidation system.

The following Ech1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ech1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22937	NM_016772.1 Latest version!	Mus musculus enoyl coenzyme A hydratase 1, peroxisomal (Ech1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ech1 ( NM_016772.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058052.1 Mus musculus enoyl coenzyme A hydratase 1, peroxisomal (Ech1), mRNA.

Price & Availability↑

CloneID	OMu22937
Clone ID Related Accession (Same CDS sequence)	NM_016772.1
Accession Version	NM_016772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF030343.1. ##Evidence-Data-START## Transcript exon combination :: AF030343.1, BC068112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACCG CGATGACAGT TTCCAGTAAA CTCCGCGGCC TGCTGATGCA GCAGCTGAGA 
GGTACCAGCC AGCTGTACTT CAACATCAGC CTTCGATCTC TGAGCTCCTC TGCACAAGAG 
GCCTCCAAGA GAGCCCCCGA GGAAGTCTCA GACCACAACT ATGAGTCTAT TCAAGTCACA 
AGTGCCCAGA AGCATGTTCT CCACGTGCAG CTAAACCGGC CAGAGAAGAG GAATGCCATG 
AACAGGGCTT TCTGGAGGGA GTTGGTGGAA TGCTTCCAAA AGATATCCAA AGACTCGGAC 
TGCCGGGCTG TTGTAGTCTC CGGTGCAGGA AAGATGTTCA CTTCAGGTAT TGACCTCATG 
GACATGGCCT CCGAACTCAT GCAGCCCTCA GGAGATGATG CGGCCCGCAT AGCCTGGTAC 
CTCCGTGACC TCATCAGCAA GTACCAGAAG ACCTTCACTG TCATTGAGAA GTGCCCCAAG 
CCAGTGATTG CTGCCATTCA CGGAGGCTGC ATTGGCGGAG GTGTGGACCT CGTTTCTGCC 
TGTGACATTC GCTACTGCAC TCAGGATGCT TTTTTCCAGA TCAAGGAGGT AGATATGGGC 
TTGGCTGCTG ATGTAGGAAC ACTGCAGAGA CTGCCCAAGG TCATCGGGAA CCAGAGCCTG 
GTCAACGAGC TGACCTTCTC TGCCCGCAAG ATGATGGCTG ACGAGGCCCT GGACAGTGGG 
CTGGTCAGCC GCGTGTTCCA AGATAAGGAC GCCATGCTGA ATGCAGCCTT TGCCCTGGCG 
GCTGATATTT CCAGCAAGAG CCCTGTGGCT GTGCAGGGGT CAAAAATCAA TCTCATCTAC 
TCCCGAGACC ATTCTGTGGA CGAGAGCCTT GACTACATGG CCACCTGGAA CATGAGCATG 
CTGCAGACCC AGGACATCAT CAAATCAGTC CAGGCAGCCA TGGAGAAGAG GGACACAAAA 
AGCATCACCT TCTCCAAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058052.1
CDS	24..1007
Translation	MATAMTVSSKLRGLLMQQLRGTSQLYFNISLRSLSSSAQEASKRAPEEVSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSGAGKMFTSGIDLMDMASELMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLVSACDIRYCTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLVNELTFSARKMMADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL

Target ORF information:

RefSeq Version	NM_016772.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus enoyl coenzyme A hydratase 1, peroxisomal (Ech1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016772.1

ORF Insert Sequence:

ATGGCTACCG CGATGACAGT TTCCAGTAAA CTCCGCGGCC TGCTGATGCA GCAGCTGAGA 
GGTACCAGCC AGCTGTACTT CAACATCAGC CTTCGATCTC TGAGCTCCTC TGCACAAGAG 
GCCTCCAAGA GAGCCCCCGA GGAAGTCTCA GACCACAACT ATGAGTCTAT TCAAGTCACA 
AGTGCCCAGA AGCATGTTCT CCACGTGCAG CTAAACCGGC CAGAGAAGAG GAATGCCATG 
AACAGGGCTT TCTGGAGGGA GTTGGTGGAA TGCTTCCAAA AGATATCCAA AGACTCGGAC 
TGCCGGGCTG TTGTAGTCTC CGGTGCAGGA AAGATGTTCA CTTCAGGTAT TGACCTCATG 
GACATGGCCT CCGAACTCAT GCAGCCCTCA GGAGATGATG CGGCCCGCAT AGCCTGGTAC 
CTCCGTGACC TCATCAGCAA GTACCAGAAG ACCTTCACTG TCATTGAGAA GTGCCCCAAG 
CCAGTGATTG CTGCCATTCA CGGAGGCTGC ATTGGCGGAG GTGTGGACCT CGTTTCTGCC 
TGTGACATTC GCTACTGCAC TCAGGATGCT TTTTTCCAGA TCAAGGAGGT AGATATGGGC 
TTGGCTGCTG ATGTAGGAAC ACTGCAGAGA CTGCCCAAGG TCATCGGGAA CCAGAGCCTG 
GTCAACGAGC TGACCTTCTC TGCCCGCAAG ATGATGGCTG ACGAGGCCCT GGACAGTGGG 
CTGGTCAGCC GCGTGTTCCA AGATAAGGAC GCCATGCTGA ATGCAGCCTT TGCCCTGGCG 
GCTGATATTT CCAGCAAGAG CCCTGTGGCT GTGCAGGGGT CAAAAATCAA TCTCATCTAC 
TCCCGAGACC ATTCTGTGGA CGAGAGCCTT GACTACATGG CCACCTGGAA CATGAGCATG 
CTGCAGACCC AGGACATCAT CAAATCAGTC CAGGCAGCCA TGGAGAAGAG GGACACAAAA 
AGCATCACCT TCTCCAAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An Ideal PPAR Response Element Bound to and Activated by PPAR?.
PloS one10(8)e0134996(2015)
Tzeng J,Byun J,Park JY,Yamamoto T,Schesing K,Tian B,Sadoshima J,Oka S

Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie67(7)557-60(2013 Sep)
Zhang J,Sun M,Li R,Liu S,Mao J,Huang Y,Wang B,Hou L,Ibrahim MM,Tang J

[Down-regulation of Ech1 decreases the adhesion ability of mouse hepatocarcinoma Hca-F cells].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology20(8)605-10(2012 Aug)
Zhang J,Tang JW,Sun MZ,Liu SQ,Song MY,Wang B,Song B,Huang YH

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Ech1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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