Cyp4f1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp4f1
Entrez Gene ID	56266
Full Name	cytochrome P450, family 4, subfamily f, polypeptide 1
Synonyms	Cyp4f14,Cyp4f2
General protein information	Preferred Names cytochrome P450, family 4, subfamily f, polypeptide 1 Names cytochrome P450 4F1 CYPIVF1 P450-A3 cytochrome P450, family 4, subfamily F, polypeptide 2 cytochrome P450, subfamily IVF, polypeptide 14 (leukotriene B4 omega hydroxylase) cytochrome P450-A3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	may be involved in hepatic carcinogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765180.2	XP_008763402.1	cytochrome P450 4F1 isoform X1
XM_008765181.1	XP_008763403.1	cytochrome P450 4F1 isoform X1
NM_019623.2	NP_062569.2	cytochrome P450 4F1

Pathways from BioSystems

Homologies

	CYP4F12	XP_001112408.1
Homo sapiens (human)	CYP4F12	NP_076433.3
Pan troglodytes (chimpanzee)	LOC468754	XP_524141.2
Rattus norvegicus (Norway rat)	Cyp4f1	NP_062569.2
Oryza sativa (rice)	Os02g0204700	NP_001172847.1
Mus musculus (house mouse)	Cyp4f14	NP_001191262.1
Arabidopsis thaliana (thale cress)	BAS1	NP_180239.1
Oryza sativa (rice)	Os06g0600400	NP_001058000.1

Gene Ontology
Bibliography

Related articles in PubMed

?-Tocopherol status and altered expression of ?-tocopherol-related proteins in streptozotocin-induced type 1 diabetes in rat models.
Takitani K, Inoue K, Koh M, Miyazaki H, Kishi K, Inoue A, Tamai H
Journal of nutritional science and vitaminology60(6)380-6(2014)

CYP4Fs expression in rat brain correlates with changes in LTB4 levels after traumatic brain injury.
Wang Y, Zhao J, Kalsotra A, Turman CM, Grill RJ, Dash PK, Strobel HW
Journal of neurotrauma25(10)1187-94(2008 Oct)

Genomic structure and regulation of the rat hepatic CYP4F1 gene by peroxisome proliferators.
Donelson E, Chen L, Zhang X, Goswami P, Song BJ, Hardwick JP
Archives of biochemistry and biophysics472(1)1-16(2008 Apr)

Localization of rat cytochrome P450 in various tissues and comparison of arachidonic acid metabolism by rat P450 with that by human P450 orthologs.
Imaoka S, Hashizume T, Funae Y
Drug metabolism and pharmacokinetics20(6)478-84(2005 Dec)

Renal localization, expression, and developmental regulation of P450 4F cytochromes in three substrains of spontaneously hypertensive rats.
Kalsotra A, Cui X, Anakk S, Hinojos CA, Doris PA, Strobel HW
Biochemical and biophysical research communications338(1)423-31(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The reduced expression of hepatic alpha-TTP and CYP4F2 genes probably leads to decreased plasma alpha-tocopherol levels and elevated alpha-tocopherol levels in the liver of streptozotocin rats.
Title: ?-Tocopherol status and altered expression of ?-tocopherol-related proteins in streptozotocin-induced type 1 diabetes in rat models.

In clofibrate treated rats HNF4alpha binds during gene repression, suggesting differential regulation of the CYP4F1 gene in vivo and in cell lines.
Title: Genomic structure and regulation of the rat hepatic CYP4F1 gene by peroxisome proliferators.

CYP4F1 is more highly expressed in the renal cortex than medulla of the spontaneously hypertensive (SHR) rat and may be a critical enzyme in the development of this rat strain.
Title: Renal localization, expression, and developmental regulation of P450 4F cytochromes in three substrains of spontaneously hypertensive rats.

The following Cyp4f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp4f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04639	NM_019623.2 Latest version!	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa04639	XM_008765180.2 Latest version!	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa04639	XM_008765181.1 Latest version!	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp4f1 ( NM_019623.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062569.2 Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), mRNA.

Price & Availability↑

CloneID	ORa04639
Clone ID Related Accession (Same CDS sequence)	XM_008765180.2 , XM_008765181.1 , NM_019623.2
Accession Version	NM_019623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 4F1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081808.1. On Sep 16, 2004 this sequence version replaced NM_019623.1. ##Evidence-Data-START## Transcript exon combination :: BC081808.1, M94548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906357, SAMN01906358 [ECO:0000348] ##Evidence-Data-END##

ATGTCACAGC TGAGCCTCTC CTGGCTGGGA CTGGGGCCTG AGGTGGCCTT CCCATGGCAG 
ACCCTGCTAC TGTTTGGAGC CTCCTGGATC CTGGCCCAAA TTCTGACCCA GATCTACGCT 
GCCTATAGGA ACTTTCGTCG CCTCCGTGGC TTCCCTCAGC CCCCCAAACG GAACTGGCTT 
ATGGGTCATG TGGGCATGGT CACTCCTACA GAGCAGGGCT TGAAGGAATT GACTCGGTTG 
GTGGGTACCT ACCCCCAAGG CTTTTTGATG TGGATTGGGC CAATGGTCCC CGTTATTACT 
CTGTGCCACT CTGACATCGT CCGATCTATC CTCAATGCCT CAGCTGCCGT TGCGCTCAAG 
GATGTGATAT TCTACACCAT CCTCAAGCCT TGGCTGGGGG ATGGGCTCCT GGTGAGTGCT 
GGTGACAAGT GGAGCCGCCA CCGCCGCATG CTGACACCTG CCTTCCACTT CAACATCCTG 
AAACCCTATG TGAAGATTTT CAACGACAGC ACCAACATCA TGCACGCCAA GTGGAAACGG 
TTGATTTCAG AAGGCAGTTC CCGTCTGGAC ATGTTTGAGC ACGTCAGCCT CATGACTCTG 
GACAGTCTGC AGAAATGTGT CTTCAGCTTT GACAGCAACT GCCAGGAGAA GTCCAGTGAA 
TATATTGCTG CCATCCTGGA GCTCAGTGCC CTAGTGGCCA AAAGGCACCA GCAGCCTTTG 
CTGTTCATGG ACCTGCTGTA CAATCTCACC CCTGATGGGA TGCGCTTCCA TAAGGCCTGC 
AACCTGGTGC ATGAATTCAC TGATGCCGTC ATCCGGGAGC GGCGCCGCAC CCTCCCAGAT 
CAGGGTTTGG ATGAGTTTCT CAAGTCTAAG GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTGCTCC TGACCAAGGA TGAAGATGGA AAGGAGCTGT CGGATGAGGA CATCAGAGCA 
GAGGCCGACA CCTTCATGTT TGAGGGCCAT GACACCACAG CCAGTGGGCT CTCCTGGATT 
CTATACAACC TGGCGAAGCA CCCAGAGTAC CAGGAGCGCT GCCGGCAGGA AGTTCAGGAG 
CTTCTGAGGG ACCGAGATCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCCC CAGTCACGGT CATCTCCCGA 
TGCTGCACCC AGGACATTCT GCTCCCAGAT GGCAGGACCA TTCCCAAAGG TATCATCTGC 
CTTATCAGTA TCTTTGGAAT TCATCACAAC CCGTCTGTGT GGCCGGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC TGAAAACATC AAAGACAGCT CACCTCTGGC ATTTATTCCC 
TTCTCGGCGG GGCCCAGGAA CTGCATAGGT CAGACTTTCG CCATGAGCGA GATGAAGGTG 
GCGCTGGCAC TAACACTGCT GCGCTTCCGA CTCCTACCGG ACGACAAGGA ACCGCGCAGG 
CAGCCAGAGC TGATCCTGCG TGCCGAGGGC GGACTGTGGC TGCGGGTGGA GCCGCTGAGC 
GCGGGCGCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062569.2
CDS	131..1705
Translation	MSQLSLSWLGLGPEVAFPWQTLLLFGASWILAQILTQIYAAYRNFRRLRGFPQPPKRNWLMGHVGMVTPTEQGLKELTRLVGTYPQGFLMWIGPMVPVITLCHSDIVRSILNASAAVALKDVIFYTILKPWLGDGLLVSAGDKWSRHRRMLTPAFHFNILKPYVKIFNDSTNIMHAKWKRLISEGSSRLDMFEHVSLMTLDSLQKCVFSFDSNCQEKSSEYIAAILELSALVAKRHQQPLLFMDLLYNLTPDGMRFHKACNLVHEFTDAVIRERRRTLPDQGLDEFLKSKAKSKTLDFIDVLLLTKDEDGKELSDEDIRAEADTFMFEGHDTTASGLSWILYNLAKHPEYQERCRQEVQELLRDRDPEEIEWDDLAQLPFLTMCIKESLRLHPPVTVISRCCTQDILLPDGRTIPKGIICLISIFGIHHNPSVWPDPEVYNPFRFDPENIKDSSPLAFIPFSAGPRNCIGQTFAMSEMKVALALTLLRFRLLPDDKEPRRQPELILRAEGGLWLRVEPLSAGAQ

Target ORF information:

RefSeq Version	NM_019623.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019623.2

ORF Insert Sequence:

ATGTCACAGC TGAGCCTCTC CTGGCTGGGA CTGGGGCCTG AGGTGGCCTT CCCATGGCAG 
ACCCTGCTAC TGTTTGGAGC CTCCTGGATC CTGGCCCAAA TTCTGACCCA GATCTACGCT 
GCCTATAGGA ACTTTCGTCG CCTCCGTGGC TTCCCTCAGC CCCCCAAACG GAACTGGCTT 
ATGGGTCATG TGGGCATGGT CACTCCTACA GAGCAGGGCT TGAAGGAATT GACTCGGTTG 
GTGGGTACCT ACCCCCAAGG CTTTTTGATG TGGATTGGGC CAATGGTCCC CGTTATTACT 
CTGTGCCACT CTGACATCGT CCGATCTATC CTCAATGCCT CAGCTGCCGT TGCGCTCAAG 
GATGTGATAT TCTACACCAT CCTCAAGCCT TGGCTGGGGG ATGGGCTCCT GGTGAGTGCT 
GGTGACAAGT GGAGCCGCCA CCGCCGCATG CTGACACCTG CCTTCCACTT CAACATCCTG 
AAACCCTATG TGAAGATTTT CAACGACAGC ACCAACATCA TGCACGCCAA GTGGAAACGG 
TTGATTTCAG AAGGCAGTTC CCGTCTGGAC ATGTTTGAGC ACGTCAGCCT CATGACTCTG 
GACAGTCTGC AGAAATGTGT CTTCAGCTTT GACAGCAACT GCCAGGAGAA GTCCAGTGAA 
TATATTGCTG CCATCCTGGA GCTCAGTGCC CTAGTGGCCA AAAGGCACCA GCAGCCTTTG 
CTGTTCATGG ACCTGCTGTA CAATCTCACC CCTGATGGGA TGCGCTTCCA TAAGGCCTGC 
AACCTGGTGC ATGAATTCAC TGATGCCGTC ATCCGGGAGC GGCGCCGCAC CCTCCCAGAT 
CAGGGTTTGG ATGAGTTTCT CAAGTCTAAG GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTGCTCC TGACCAAGGA TGAAGATGGA AAGGAGCTGT CGGATGAGGA CATCAGAGCA 
GAGGCCGACA CCTTCATGTT TGAGGGCCAT GACACCACAG CCAGTGGGCT CTCCTGGATT 
CTATACAACC TGGCGAAGCA CCCAGAGTAC CAGGAGCGCT GCCGGCAGGA AGTTCAGGAG 
CTTCTGAGGG ACCGAGATCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCCC CAGTCACGGT CATCTCCCGA 
TGCTGCACCC AGGACATTCT GCTCCCAGAT GGCAGGACCA TTCCCAAAGG TATCATCTGC 
CTTATCAGTA TCTTTGGAAT TCATCACAAC CCGTCTGTGT GGCCGGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC TGAAAACATC AAAGACAGCT CACCTCTGGC ATTTATTCCC 
TTCTCGGCGG GGCCCAGGAA CTGCATAGGT CAGACTTTCG CCATGAGCGA GATGAAGGTG 
GCGCTGGCAC TAACACTGCT GCGCTTCCGA CTCCTACCGG ACGACAAGGA ACCGCGCAGG 
CAGCCAGAGC TGATCCTGCG TGCCGAGGGC GGACTGTGGC TGCGGGTGGA GCCGCTGAGC 
GCGGGCGCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp4f1 ( XM_008765180.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763402.1 Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04639
Clone ID Related Accession (Same CDS sequence)	XM_008765180.2 , XM_008765181.1 , NM_019623.2
Accession Version	XM_008765180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 4F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGC TGAGCCTCTC CTGGCTGGGA CTGGGGCCTG AGGTGGCCTT CCCATGGCAG 
ACCCTGCTAC TGTTTGGAGC CTCCTGGATC CTGGCCCAAA TTCTGACCCA GATCTACGCT 
GCCTATAGGA ACTTTCGTCG CCTCCGTGGC TTCCCTCAGC CCCCCAAACG GAACTGGCTT 
ATGGGTCATG TGGGCATGGT CACTCCTACA GAGCAGGGCT TGAAGGAATT GACTCGGTTG 
GTGGGTACCT ACCCCCAAGG CTTTTTGATG TGGATTGGGC CAATGGTCCC CGTTATTACT 
CTGTGCCACT CTGACATCGT CCGATCTATC CTCAATGCCT CAGCTGCCGT TGCGCTCAAG 
GATGTGATAT TCTACACCAT CCTCAAGCCT TGGCTGGGGG ATGGGCTCCT GGTGAGTGCT 
GGTGACAAGT GGAGCCGCCA CCGCCGCATG CTGACACCTG CCTTCCACTT CAACATCCTG 
AAACCCTATG TGAAGATTTT CAACGACAGC ACCAACATCA TGCACGCCAA GTGGAAACGG 
TTGATTTCAG AAGGCAGTTC CCGTCTGGAC ATGTTTGAGC ACGTCAGCCT CATGACTCTG 
GACAGTCTGC AGAAATGTGT CTTCAGCTTT GACAGCAACT GCCAGGAGAA GTCCAGTGAA 
TATATTGCTG CCATCCTGGA GCTCAGTGCC CTAGTGGCCA AAAGGCACCA GCAGCCTTTG 
CTGTTCATGG ACCTGCTGTA CAATCTCACC CCTGATGGGA TGCGCTTCCA TAAGGCCTGC 
AACCTGGTGC ATGAATTCAC TGATGCCGTC ATCCGGGAGC GGCGCCGCAC CCTCCCAGAT 
CAGGGTTTGG ATGAGTTTCT CAAGTCTAAG GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTGCTCC TGACCAAGGA TGAAGATGGA AAGGAGCTGT CGGATGAGGA CATCAGAGCA 
GAGGCCGACA CCTTCATGTT TGAGGGCCAT GACACCACAG CCAGTGGGCT CTCCTGGATT 
CTATACAACC TGGCGAAGCA CCCAGAGTAC CAGGAGCGCT GCCGGCAGGA AGTTCAGGAG 
CTTCTGAGGG ACCGAGATCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCCC CAGTCACGGT CATCTCCCGA 
TGCTGCACCC AGGACATTCT GCTCCCAGAT GGCAGGACCA TTCCCAAAGG TATCATCTGC 
CTTATCAGTA TCTTTGGAAT TCATCACAAC CCGTCTGTGT GGCCGGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC TGAAAACATC AAAGACAGCT CACCTCTGGC ATTTATTCCC 
TTCTCGGCGG GGCCCAGGAA CTGCATAGGT CAGACTTTCG CCATGAGCGA GATGAAGGTG 
GCGCTGGCAC TAACACTGCT GCGCTTCCGA CTCCTACCGG ACGACAAGGA ACCGCGCAGG 
CAGCCAGAGC TGATCCTGCG TGCCGAGGGC GGACTGTGGC TGCGGGTGGA GCCGCTGAGC 
GCGGGCGCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763402.1
CDS	84..1658
Translation	MSQLSLSWLGLGPEVAFPWQTLLLFGASWILAQILTQIYAAYRNFRRLRGFPQPPKRNWLMGHVGMVTPTEQGLKELTRLVGTYPQGFLMWIGPMVPVITLCHSDIVRSILNASAAVALKDVIFYTILKPWLGDGLLVSAGDKWSRHRRMLTPAFHFNILKPYVKIFNDSTNIMHAKWKRLISEGSSRLDMFEHVSLMTLDSLQKCVFSFDSNCQEKSSEYIAAILELSALVAKRHQQPLLFMDLLYNLTPDGMRFHKACNLVHEFTDAVIRERRRTLPDQGLDEFLKSKAKSKTLDFIDVLLLTKDEDGKELSDEDIRAEADTFMFEGHDTTASGLSWILYNLAKHPEYQERCRQEVQELLRDRDPEEIEWDDLAQLPFLTMCIKESLRLHPPVTVISRCCTQDILLPDGRTIPKGIICLISIFGIHHNPSVWPDPEVYNPFRFDPENIKDSSPLAFIPFSAGPRNCIGQTFAMSEMKVALALTLLRFRLLPDDKEPRRQPELILRAEGGLWLRVEPLSAGAQ

Target ORF information:

RefSeq Version	XM_008765180.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765180.2

ORF Insert Sequence:

ATGTCACAGC TGAGCCTCTC CTGGCTGGGA CTGGGGCCTG AGGTGGCCTT CCCATGGCAG 
ACCCTGCTAC TGTTTGGAGC CTCCTGGATC CTGGCCCAAA TTCTGACCCA GATCTACGCT 
GCCTATAGGA ACTTTCGTCG CCTCCGTGGC TTCCCTCAGC CCCCCAAACG GAACTGGCTT 
ATGGGTCATG TGGGCATGGT CACTCCTACA GAGCAGGGCT TGAAGGAATT GACTCGGTTG 
GTGGGTACCT ACCCCCAAGG CTTTTTGATG TGGATTGGGC CAATGGTCCC CGTTATTACT 
CTGTGCCACT CTGACATCGT CCGATCTATC CTCAATGCCT CAGCTGCCGT TGCGCTCAAG 
GATGTGATAT TCTACACCAT CCTCAAGCCT TGGCTGGGGG ATGGGCTCCT GGTGAGTGCT 
GGTGACAAGT GGAGCCGCCA CCGCCGCATG CTGACACCTG CCTTCCACTT CAACATCCTG 
AAACCCTATG TGAAGATTTT CAACGACAGC ACCAACATCA TGCACGCCAA GTGGAAACGG 
TTGATTTCAG AAGGCAGTTC CCGTCTGGAC ATGTTTGAGC ACGTCAGCCT CATGACTCTG 
GACAGTCTGC AGAAATGTGT CTTCAGCTTT GACAGCAACT GCCAGGAGAA GTCCAGTGAA 
TATATTGCTG CCATCCTGGA GCTCAGTGCC CTAGTGGCCA AAAGGCACCA GCAGCCTTTG 
CTGTTCATGG ACCTGCTGTA CAATCTCACC CCTGATGGGA TGCGCTTCCA TAAGGCCTGC 
AACCTGGTGC ATGAATTCAC TGATGCCGTC ATCCGGGAGC GGCGCCGCAC CCTCCCAGAT 
CAGGGTTTGG ATGAGTTTCT CAAGTCTAAG GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTGCTCC TGACCAAGGA TGAAGATGGA AAGGAGCTGT CGGATGAGGA CATCAGAGCA 
GAGGCCGACA CCTTCATGTT TGAGGGCCAT GACACCACAG CCAGTGGGCT CTCCTGGATT 
CTATACAACC TGGCGAAGCA CCCAGAGTAC CAGGAGCGCT GCCGGCAGGA AGTTCAGGAG 
CTTCTGAGGG ACCGAGATCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCCC CAGTCACGGT CATCTCCCGA 
TGCTGCACCC AGGACATTCT GCTCCCAGAT GGCAGGACCA TTCCCAAAGG TATCATCTGC 
CTTATCAGTA TCTTTGGAAT TCATCACAAC CCGTCTGTGT GGCCGGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC TGAAAACATC AAAGACAGCT CACCTCTGGC ATTTATTCCC 
TTCTCGGCGG GGCCCAGGAA CTGCATAGGT CAGACTTTCG CCATGAGCGA GATGAAGGTG 
GCGCTGGCAC TAACACTGCT GCGCTTCCGA CTCCTACCGG ACGACAAGGA ACCGCGCAGG 
CAGCCAGAGC TGATCCTGCG TGCCGAGGGC GGACTGTGGC TGCGGGTGGA GCCGCTGAGC 
GCGGGCGCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp4f1 ( XM_008765181.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763403.1 Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa04639
Clone ID Related Accession (Same CDS sequence)	XM_008765180.2 , XM_008765181.1 , NM_019623.2
Accession Version	XM_008765181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 4F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGC TGAGCCTCTC CTGGCTGGGA CTGGGGCCTG AGGTGGCCTT CCCATGGCAG 
ACCCTGCTAC TGTTTGGAGC CTCCTGGATC CTGGCCCAAA TTCTGACCCA GATCTACGCT 
GCCTATAGGA ACTTTCGTCG CCTCCGTGGC TTCCCTCAGC CCCCCAAACG GAACTGGCTT 
ATGGGTCATG TGGGCATGGT CACTCCTACA GAGCAGGGCT TGAAGGAATT GACTCGGTTG 
GTGGGTACCT ACCCCCAAGG CTTTTTGATG TGGATTGGGC CAATGGTCCC CGTTATTACT 
CTGTGCCACT CTGACATCGT CCGATCTATC CTCAATGCCT CAGCTGCCGT TGCGCTCAAG 
GATGTGATAT TCTACACCAT CCTCAAGCCT TGGCTGGGGG ATGGGCTCCT GGTGAGTGCT 
GGTGACAAGT GGAGCCGCCA CCGCCGCATG CTGACACCTG CCTTCCACTT CAACATCCTG 
AAACCCTATG TGAAGATTTT CAACGACAGC ACCAACATCA TGCACGCCAA GTGGAAACGG 
TTGATTTCAG AAGGCAGTTC CCGTCTGGAC ATGTTTGAGC ACGTCAGCCT CATGACTCTG 
GACAGTCTGC AGAAATGTGT CTTCAGCTTT GACAGCAACT GCCAGGAGAA GTCCAGTGAA 
TATATTGCTG CCATCCTGGA GCTCAGTGCC CTAGTGGCCA AAAGGCACCA GCAGCCTTTG 
CTGTTCATGG ACCTGCTGTA CAATCTCACC CCTGATGGGA TGCGCTTCCA TAAGGCCTGC 
AACCTGGTGC ATGAATTCAC TGATGCCGTC ATCCGGGAGC GGCGCCGCAC CCTCCCAGAT 
CAGGGTTTGG ATGAGTTTCT CAAGTCTAAG GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTGCTCC TGACCAAGGA TGAAGATGGA AAGGAGCTGT CGGATGAGGA CATCAGAGCA 
GAGGCCGACA CCTTCATGTT TGAGGGCCAT GACACCACAG CCAGTGGGCT CTCCTGGATT 
CTATACAACC TGGCGAAGCA CCCAGAGTAC CAGGAGCGCT GCCGGCAGGA AGTTCAGGAG 
CTTCTGAGGG ACCGAGATCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCCC CAGTCACGGT CATCTCCCGA 
TGCTGCACCC AGGACATTCT GCTCCCAGAT GGCAGGACCA TTCCCAAAGG TATCATCTGC 
CTTATCAGTA TCTTTGGAAT TCATCACAAC CCGTCTGTGT GGCCGGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC TGAAAACATC AAAGACAGCT CACCTCTGGC ATTTATTCCC 
TTCTCGGCGG GGCCCAGGAA CTGCATAGGT CAGACTTTCG CCATGAGCGA GATGAAGGTG 
GCGCTGGCAC TAACACTGCT GCGCTTCCGA CTCCTACCGG ACGACAAGGA ACCGCGCAGG 
CAGCCAGAGC TGATCCTGCG TGCCGAGGGC GGACTGTGGC TGCGGGTGGA GCCGCTGAGC 
GCGGGCGCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763403.1
CDS	25..1599
Translation	MSQLSLSWLGLGPEVAFPWQTLLLFGASWILAQILTQIYAAYRNFRRLRGFPQPPKRNWLMGHVGMVTPTEQGLKELTRLVGTYPQGFLMWIGPMVPVITLCHSDIVRSILNASAAVALKDVIFYTILKPWLGDGLLVSAGDKWSRHRRMLTPAFHFNILKPYVKIFNDSTNIMHAKWKRLISEGSSRLDMFEHVSLMTLDSLQKCVFSFDSNCQEKSSEYIAAILELSALVAKRHQQPLLFMDLLYNLTPDGMRFHKACNLVHEFTDAVIRERRRTLPDQGLDEFLKSKAKSKTLDFIDVLLLTKDEDGKELSDEDIRAEADTFMFEGHDTTASGLSWILYNLAKHPEYQERCRQEVQELLRDRDPEEIEWDDLAQLPFLTMCIKESLRLHPPVTVISRCCTQDILLPDGRTIPKGIICLISIFGIHHNPSVWPDPEVYNPFRFDPENIKDSSPLAFIPFSAGPRNCIGQTFAMSEMKVALALTLLRFRLLPDDKEPRRQPELILRAEGGLWLRVEPLSAGAQ

Target ORF information:

RefSeq Version	XM_008765181.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1 (Cyp4f1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765181.1

ORF Insert Sequence:

ATGTCACAGC TGAGCCTCTC CTGGCTGGGA CTGGGGCCTG AGGTGGCCTT CCCATGGCAG 
ACCCTGCTAC TGTTTGGAGC CTCCTGGATC CTGGCCCAAA TTCTGACCCA GATCTACGCT 
GCCTATAGGA ACTTTCGTCG CCTCCGTGGC TTCCCTCAGC CCCCCAAACG GAACTGGCTT 
ATGGGTCATG TGGGCATGGT CACTCCTACA GAGCAGGGCT TGAAGGAATT GACTCGGTTG 
GTGGGTACCT ACCCCCAAGG CTTTTTGATG TGGATTGGGC CAATGGTCCC CGTTATTACT 
CTGTGCCACT CTGACATCGT CCGATCTATC CTCAATGCCT CAGCTGCCGT TGCGCTCAAG 
GATGTGATAT TCTACACCAT CCTCAAGCCT TGGCTGGGGG ATGGGCTCCT GGTGAGTGCT 
GGTGACAAGT GGAGCCGCCA CCGCCGCATG CTGACACCTG CCTTCCACTT CAACATCCTG 
AAACCCTATG TGAAGATTTT CAACGACAGC ACCAACATCA TGCACGCCAA GTGGAAACGG 
TTGATTTCAG AAGGCAGTTC CCGTCTGGAC ATGTTTGAGC ACGTCAGCCT CATGACTCTG 
GACAGTCTGC AGAAATGTGT CTTCAGCTTT GACAGCAACT GCCAGGAGAA GTCCAGTGAA 
TATATTGCTG CCATCCTGGA GCTCAGTGCC CTAGTGGCCA AAAGGCACCA GCAGCCTTTG 
CTGTTCATGG ACCTGCTGTA CAATCTCACC CCTGATGGGA TGCGCTTCCA TAAGGCCTGC 
AACCTGGTGC ATGAATTCAC TGATGCCGTC ATCCGGGAGC GGCGCCGCAC CCTCCCAGAT 
CAGGGTTTGG ATGAGTTTCT CAAGTCTAAG GCCAAGTCCA AGACTTTGGA TTTCATTGAT 
GTGCTGCTCC TGACCAAGGA TGAAGATGGA AAGGAGCTGT CGGATGAGGA CATCAGAGCA 
GAGGCCGACA CCTTCATGTT TGAGGGCCAT GACACCACAG CCAGTGGGCT CTCCTGGATT 
CTATACAACC TGGCGAAGCA CCCAGAGTAC CAGGAGCGCT GCCGGCAGGA AGTTCAGGAG 
CTTCTGAGGG ACCGAGATCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTGACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCCC CAGTCACGGT CATCTCCCGA 
TGCTGCACCC AGGACATTCT GCTCCCAGAT GGCAGGACCA TTCCCAAAGG TATCATCTGC 
CTTATCAGTA TCTTTGGAAT TCATCACAAC CCGTCTGTGT GGCCGGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC TGAAAACATC AAAGACAGCT CACCTCTGGC ATTTATTCCC 
TTCTCGGCGG GGCCCAGGAA CTGCATAGGT CAGACTTTCG CCATGAGCGA GATGAAGGTG 
GCGCTGGCAC TAACACTGCT GCGCTTCCGA CTCCTACCGG ACGACAAGGA ACCGCGCAGG 
CAGCCAGAGC TGATCCTGCG TGCCGAGGGC GGACTGTGGC TGCGGGTGGA GCCGCTGAGC 
GCGGGCGCGC AGTGA