Pir cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pir
Entrez Gene ID	363465
Full Name	pirin
General protein information	Preferred Names pirin Names pirin pirin (iron-binding nuclear protein) probable quercetin 2,3-dioxygenase PIR probable quercetinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009474.1	NP_001009474.1	pirin

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIR	NP_003653.1
Arabidopsis thaliana (thale cress)	AT2G43120	NP_850385.1
Bos taurus (cattle)	PIR	NP_001095828.1
Mus musculus (house mouse)	Pir	NP_081429.1
Danio rerio (zebrafish)	pir	NP_001002550.1
Kluyveromyces lactis	KLLA0D01243g	XP_453124.1
Oryza sativa (rice)	Os03g0845000	NP_001051876.1
Rattus norvegicus (Norway rat)	Pir	NP_001009474.1
Arabidopsis thaliana (thale cress)	PRN	NP_191481.1
	PIR	XP_002806203.1
Canis lupus familiaris (dog)	PIR	XP_005641188.1
Arabidopsis thaliana (thale cress)	AT3G59260	NP_191485.1
Pan troglodytes (chimpanzee)	PIR	XP_003317418.2
Magnaporthe oryzae (rice blast fungus)	MGG_02400	XP_003709181.1
Neurospora crassa	NCU03369	XP_956304.1
Xenopus tropicalis (tropical clawed frog)	pir	NP_001011440.1

Gene Ontology
Bibliography

Related articles in PubMed

Pirin is an iron-dependent redox regulator of NF-?B.
Liu F, Rehmani I, Esaki S, Fu R, Chen L, de Serrano V, Liu A
Proceedings of the National Academy of Sciences of the United States of America110(24)9722-7(2013 Jun)

A small-molecule inhibitor shows that pirin regulates migration of melanoma cells.
Miyazaki I, Simizu S, Okumura H, Takagi S, Osada H
Nature chemical biology6(9)667-73(2010 Sep)

Pirin downregulation is a feature of AML and leads to impairment of terminal myeloid differentiation.
Licciulli S, Cambiaghi V, Scafetta G, Gruszka AM, Alcalay M
Leukemia24(2)429-37(2010 Feb)

Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches.
Licciulli S, Luise C, Zanardi A, Giorgetti L, Viale G, Lanfrancone L, Carbone R, Alcalay M
BMC cell biology115(2010 Jan)

Structural and biochemical analysis reveal pirins to possess quercetinase activity.
Adams M, Jia Z
The Journal of biological chemistry280(31)28675-82(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pir gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pir cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00971	NM_001009474.1 Latest version!	Rattus norvegicus pirin (Pir), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pir ( NM_001009474.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009474.1 Rattus norvegicus pirin (Pir), mRNA.

Price & Availability↑

CloneID	ORa00971
Clone ID Related Accession (Same CDS sequence)	NM_001009474.1
Accession Version	NM_001009474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	pirin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088290.1. On Jan 7, 2005 this sequence version replaced XM_343784.1. ##Evidence-Data-START## Transcript exon combination :: BC088290.1, CF109436.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN01906356 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCT CTAAAAAGGT TACTCTCTCC GTCCTCAGCC GGGAACAGTC AGAGGGGGTT 
GGAGCACGGG TGCGCAGAAG CATTGGCGGA CCTGAGTTGA AAATGCTGGA TCCATTTTTG 
CTGTTTGATG AATTTAAAGG AGGAAGGCCG GGAGGATTTC CTGATCATCC ACACCGAGGA 
TTTGAAACAG TATCTTATCT TCTAGAAGGT GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CATGTTGGTA AAATGAACCC AGGAGACCTA CAGTGGATGA CTGCAGGACG AGGCATTCTA 
CATGCTGAGA TGCCCTGCTC AGAGGAGCCA GCCCATGGCT TACAGCTCTG GGTGAATTTG 
AAACGCTCAG AAAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAGATCCCG 
AAACCCAGCA AAGATGGTGT GACAGTCGCT GTCATTTCAG GAGAAGCCTT GGGAATAAAG 
TCGAAGGTTT ACACTCGCAC ACCAACCTTA TATCTGGACT TCAAGTTGGA CCAAGGAGCA 
AAGCATTCCC AGCCTATTCC TAAAGGGTGG ACAAGCTTCA TTTATACCAT CTCTGGAGAT 
GTGTATATTG GGCCTGATGA TGCTCAACAA AAGATAGAAC CGCATCGCAC AGCAGTGCTT 
GGAGAAGGTG ACACTGTCCA GTTGGAAAAT AAGGATCCCA AAAGAAGTCA TTTTGTCTTA 
ATTGCCGGGG AGCCATTAAG AGAACCAGTT GTCCAACATG GTCCGTTTGT GATGAATACC 
AATGAGGAGA TTTCTGAGGC CATTCTTGAT TTCAGGAATG CAAAAAATGG TTTTGAGGGG 
GCCAAAACCT GGAAGTCAAA GATTGGAAAC CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009474.1
CDS	141..1016
Translation	MASSKKVTLSVLSREQSEGVGARVRRSIGGPELKMLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHVGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLKRSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDQGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHRTAVLGEGDTVQLENKDPKRSHFVLIAGEPLREPVVQHGPFVMNTNEEISEAILDFRNAKNGFEGAKTWKSKIGNQ

Target ORF information:

RefSeq Version	NM_001009474.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pirin (Pir), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009474.1

ORF Insert Sequence:

ATGGCGTCCT CTAAAAAGGT TACTCTCTCC GTCCTCAGCC GGGAACAGTC AGAGGGGGTT 
GGAGCACGGG TGCGCAGAAG CATTGGCGGA CCTGAGTTGA AAATGCTGGA TCCATTTTTG 
CTGTTTGATG AATTTAAAGG AGGAAGGCCG GGAGGATTTC CTGATCATCC ACACCGAGGA 
TTTGAAACAG TATCTTATCT TCTAGAAGGT GGCAGCATGG CCCATGAAGA CTTCTGTGGA 
CATGTTGGTA AAATGAACCC AGGAGACCTA CAGTGGATGA CTGCAGGACG AGGCATTCTA 
CATGCTGAGA TGCCCTGCTC AGAGGAGCCA GCCCATGGCT TACAGCTCTG GGTGAATTTG 
AAACGCTCAG AAAAGATGGT GGAGCCTCAG TACCAGGAAC TGAAAAGTGA AGAGATCCCG 
AAACCCAGCA AAGATGGTGT GACAGTCGCT GTCATTTCAG GAGAAGCCTT GGGAATAAAG 
TCGAAGGTTT ACACTCGCAC ACCAACCTTA TATCTGGACT TCAAGTTGGA CCAAGGAGCA 
AAGCATTCCC AGCCTATTCC TAAAGGGTGG ACAAGCTTCA TTTATACCAT CTCTGGAGAT 
GTGTATATTG GGCCTGATGA TGCTCAACAA AAGATAGAAC CGCATCGCAC AGCAGTGCTT 
GGAGAAGGTG ACACTGTCCA GTTGGAAAAT AAGGATCCCA AAAGAAGTCA TTTTGTCTTA 
ATTGCCGGGG AGCCATTAAG AGAACCAGTT GTCCAACATG GTCCGTTTGT GATGAATACC 
AATGAGGAGA TTTCTGAGGC CATTCTTGAT TTCAGGAATG CAAAAAATGG TTTTGAGGGG 
GCCAAAACCT GGAAGTCAAA GATTGGAAAC CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pirin is an iron-dependent redox regulator of NF-?B.
Proceedings of the National Academy of Sciences of the United States of America110(24)9722-7(2013 Jun)
Liu F,Rehmani I,Esaki S,Fu R,Chen L,de Serrano V,Liu A

A small-molecule inhibitor shows that pirin regulates migration of melanoma cells.
Nature chemical biology6(9)667-73(2010 Sep)
Miyazaki I,Simizu S,Okumura H,Takagi S,Osada H

Pirin downregulation is a feature of AML and leads to impairment of terminal myeloid differentiation.
Leukemia24(2)429-37(2010 Feb)
Licciulli S,Cambiaghi V,Scafetta G,Gruszka AM,Alcalay M

Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches.
BMC cell biology115(2010 Jan)
Licciulli S,Luise C,Zanardi A,Giorgetti L,Viale G,Lanfrancone L,Carbone R,Alcalay M

Structural and biochemical analysis reveal pirins to possess quercetinase activity.
The Journal of biological chemistry280(31)28675-82(2005 Aug)
Adams M,Jia Z