Degs2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Degs2
Entrez Gene ID	314438
Full Name	delta(4)-desaturase, sphingolipid 2
Synonyms	RGD1305023
General protein information	Preferred Names delta(4)-desaturase, sphingolipid 2 Names sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 sphingolipid 4-desaturase sphingolipid C4-hydroxylase/delta 4-desaturase sphingolipid C4-monooxygenase sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017457.2	NP_001017457.1	sphingolipid delta(4)-desaturase/C4-monooxygenase DES2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	degs2	NP_001156838.1
Caenorhabditis elegans (roundworm)	F33D4.4	NP_501256.1
Magnaporthe oryzae (rice blast fungus)	MGG_13187	XP_003712066.1
Pan troglodytes (chimpanzee)	DEGS2	XP_001157834.1
	LOC717222	XP_001108524.1
Canis lupus familiaris (dog)	DEGS2	XP_547976.2
Mus musculus (house mouse)	Degs2	NP_081575.2
Kluyveromyces lactis	KLLA0F18656g	XP_455916.1
Bos taurus (cattle)	DEGS2	NP_001069719.1
Drosophila melanogaster (fruit fly)	ifc	NP_476594.1
Caenorhabditis elegans (roundworm)	ttm-5	NP_493549.1
Oryza sativa (rice)	Os02g0639600	NP_001047540.1
Xenopus tropicalis (tropical clawed frog)	degs2	XP_002936627.1
Homo sapiens (human)	DEGS2	NP_996801.2
Rattus norvegicus (Norway rat)	Degs2	NP_001017457.1
Gallus gallus (chicken)	DEGS2	XP_421364.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011402	XP_317914.3
Eremothecium gossypii	AGOS_AGR025W	NP_986690.2
Schizosaccharomyces pombe (fission yeast)	dsd1	NP_596407.1
Neurospora crassa	NCU08927	XP_960609.1
Arabidopsis thaliana (thale cress)	DES-1-LIKE	NP_192402.1

Gene Ontology
Bibliography

Related articles in PubMed

Dihydroceramide-based response to hypoxia.
Devlin CM, Lahm T, Hubbard WC, Van Demark M, Wang KC, Wu X, Bielawska A, Obeid LM, Ivan M, Petrache I
The Journal of biological chemistry286(44)38069-78(2011 Nov)

Myristic acid increases the activity of dihydroceramide Delta4-desaturase 1 through its N-terminal myristoylation.
Beauchamp E, Goenaga D, Le Bloc'h J, Catheline D, Legrand P, Rioux V
Biochimie89(12)1553-61(2007 Dec)

Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.
Enomoto A, Omae F, Miyazaki M, Kozutsumi Y, Yubisui T, Suzuki A
The Biochemical journal397(2)289-95(2006 Jul)

Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.
Omae F, Miyazaki M, Enomoto A, Suzuki A
FEBS letters576(1-2)63-7(2004 Oct)

Identification and characterization of a sphingolipid delta 4-desaturase family.
Ternes P, Franke S, Z?hringer U, Sperling P, Heinz E
The Journal of biological chemistry277(28)25512-8(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of DEGS1 or DEGS2 attenuates the DHC accumulation and increased cell proliferation during hypoxia
Title: Dihydroceramide-based response to hypoxia.

The following Degs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Degs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05887	NM_001017457.2 Latest version!	Rattus norvegicus delta(4)-desaturase, sphingolipid 2 (Degs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Degs2 ( NM_001017457.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001017457.1 Rattus norvegicus delta(4)-desaturase, sphingolipid 2 (Degs2), mRNA.

Price & Availability↑

CloneID	ORa05887
Clone ID Related Accession (Same CDS sequence)	NM_001017457.2
Accession Version	NM_001017457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Rattus norvegicus(Norway rat)
Product	sphingolipid delta(4)-desaturase/C4-monooxygenase DES2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098701.1. On Dec 15, 2005 this sequence version replaced NM_001017457.1. ##Evidence-Data-START## Transcript exon combination :: BC098701.1, AJ938082.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAACA GCGCAGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC 
CAGAGGCGCA AAGAGATGCT CGCCAAGTAC CCATCCATCA AGGCCCTGAT GCGGCCAGAC 
CCCAACATCA AGTGGACCGT GCTGGGAATG GTACTGGTGC AGGTGCTGGC CTGCTGGCTG 
GTACGGGGGC TCTCTTGGCG ATGGTTGCTG TTTTGGGCCT ATGCCTTTGG CGGCTGCATC 
AACCACTCAC TGACACTGGC CATCCATGAC ATCTCGCACA ACACTGCCTT TGGCACACGC 
TGTGCCTCCC GCAACCGCTG GTTTGCCGTC TTTGCTAATC TGCCCATTGG CCTACCTTAC 
GCCACGTCCT TCAAAAAGTA CCACGTGGAC CACCATCGTT ACCTGGGCGG AGACGGGCTG 
GATGTGGATA TCCCCACAGA CTTCGAGGGC TGGTTCTTCT GCACACCGGC CCGCAAGCTG 
CTTTGGCTGG TGCTTCAACC CTTCTTCTAC TCACTCAGGC CACTCTATGT GAACCCCAAG 
GCGGTGACGC GTATGGAGAT CCTCAATGCC CTGGTGCAGT TGGCATTTAA TGTCACCATC 
TTTGCCCTCT GGGGGATCAA AGCCATAGTC TACCTTCTAG CCAGCTCTCT CCTGGGCCTG 
GGCCTGCACC CTATCTCTGG CCACTTCGTG GCAGAGCATT ATATGTTCCT GAAGGGCCAC 
GAGACGTACT CCTACTATGG GCCTCTCAAC TGGATTACCT TCAATGTTGG CTACCACGTG 
GAACACCACG ACTTCCCCAG CATCCCCGGT TGCTACCTGC CACTGGTACG GATGATTGCA 
CCCGAATACT ACGACCATCT GCCGCAGCAC CACTCCTGGG TGAAAGTGCT CTGGGACTTC 
GTGTTTGAGG ACTCTCTGGG ACCCTACTCC AGGGTTAAGC GCAAGTGCAA GCTGGCGAAG 
GACCAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017457.1
CDS	68..1039
Translation	MGNSAARSDFEWVYSDQPHTQRRKEMLAKYPSIKALMRPDPNIKWTVLGMVLVQVLACWLVRGLSWRWLLFWAYAFGGCINHSLTLAIHDISHNTAFGTRCASRNRWFAVFANLPIGLPYATSFKKYHVDHHRYLGGDGLDVDIPTDFEGWFFCTPARKLLWLVLQPFFYSLRPLYVNPKAVTRMEILNALVQLAFNVTIFALWGIKAIVYLLASSLLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHVEHHDFPSIPGCYLPLVRMIAPEYYDHLPQHHSWVKVLWDFVFEDSLGPYSRVKRKCKLAKDQL

Target ORF information:

RefSeq Version	NM_001017457.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus delta(4)-desaturase, sphingolipid 2 (Degs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017457.2

ORF Insert Sequence:

ATGGGTAACA GCGCAGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC 
CAGAGGCGCA AAGAGATGCT CGCCAAGTAC CCATCCATCA AGGCCCTGAT GCGGCCAGAC 
CCCAACATCA AGTGGACCGT GCTGGGAATG GTACTGGTGC AGGTGCTGGC CTGCTGGCTG 
GTACGGGGGC TCTCTTGGCG ATGGTTGCTG TTTTGGGCCT ATGCCTTTGG CGGCTGCATC 
AACCACTCAC TGACACTGGC CATCCATGAC ATCTCGCACA ACACTGCCTT TGGCACACGC 
TGTGCCTCCC GCAACCGCTG GTTTGCCGTC TTTGCTAATC TGCCCATTGG CCTACCTTAC 
GCCACGTCCT TCAAAAAGTA CCACGTGGAC CACCATCGTT ACCTGGGCGG AGACGGGCTG 
GATGTGGATA TCCCCACAGA CTTCGAGGGC TGGTTCTTCT GCACACCGGC CCGCAAGCTG 
CTTTGGCTGG TGCTTCAACC CTTCTTCTAC TCACTCAGGC CACTCTATGT GAACCCCAAG 
GCGGTGACGC GTATGGAGAT CCTCAATGCC CTGGTGCAGT TGGCATTTAA TGTCACCATC 
TTTGCCCTCT GGGGGATCAA AGCCATAGTC TACCTTCTAG CCAGCTCTCT CCTGGGCCTG 
GGCCTGCACC CTATCTCTGG CCACTTCGTG GCAGAGCATT ATATGTTCCT GAAGGGCCAC 
GAGACGTACT CCTACTATGG GCCTCTCAAC TGGATTACCT TCAATGTTGG CTACCACGTG 
GAACACCACG ACTTCCCCAG CATCCCCGGT TGCTACCTGC CACTGGTACG GATGATTGCA 
CCCGAATACT ACGACCATCT GCCGCAGCAC CACTCCTGGG TGAAAGTGCT CTGGGACTTC 
GTGTTTGAGG ACTCTCTGGG ACCCTACTCC AGGGTTAAGC GCAAGTGCAA GCTGGCGAAG 
GACCAGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dihydroceramide-based response to hypoxia.
The Journal of biological chemistry286(44)38069-78(2011 Nov)
Devlin CM,Lahm T,Hubbard WC,Van Demark M,Wang KC,Wu X,Bielawska A,Obeid LM,Ivan M,Petrache I

Myristic acid increases the activity of dihydroceramide Delta4-desaturase 1 through its N-terminal myristoylation.
Biochimie89(12)1553-61(2007 Dec)
Beauchamp E,Goenaga D,Le Bloc'h J,Catheline D,Legrand P,Rioux V

Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.
The Biochemical journal397(2)289-95(2006 Jul)
Enomoto A,Omae F,Miyazaki M,Kozutsumi Y,Yubisui T,Suzuki A

Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.
FEBS letters576(1-2)63-7(2004 Oct)
Omae F,Miyazaki M,Enomoto A,Suzuki A

Identification and characterization of a sphingolipid delta 4-desaturase family.
The Journal of biological chemistry277(28)25512-8(2002 Jul)
Ternes P,Franke S,Z?hringer U,Sperling P,Heinz E