Degs2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Degs2
Entrez Gene ID	70059
Full Name	delta(4)-desaturase, sphingolipid 2
Synonyms	2210008A03Rik,AI852933,DES2
General protein information	Preferred Names delta(4)-desaturase, sphingolipid 2 Names sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 degenerative spermatocyte homolog 2, lipid desaturase degs2 sphingolipid 4-desaturase sphingolipid C4-monooxygenase sphingolipid delta 4 desaturase/C-4 hydroxylase sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001171002.1	NP_001164473.1	sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 2
NM_027299.5	NP_081575.2	sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	degs2	NP_001156838.1
Caenorhabditis elegans (roundworm)	F33D4.4	NP_501256.1
Magnaporthe oryzae (rice blast fungus)	MGG_13187	XP_003712066.1
Pan troglodytes (chimpanzee)	DEGS2	XP_001157834.1
	LOC717222	XP_001108524.1
Canis lupus familiaris (dog)	DEGS2	XP_547976.2
Mus musculus (house mouse)	Degs2	NP_081575.2
Kluyveromyces lactis	KLLA0F18656g	XP_455916.1
Bos taurus (cattle)	DEGS2	NP_001069719.1
Drosophila melanogaster (fruit fly)	ifc	NP_476594.1
Caenorhabditis elegans (roundworm)	ttm-5	NP_493549.1
Oryza sativa (rice)	Os02g0639600	NP_001047540.1
Xenopus tropicalis (tropical clawed frog)	degs2	XP_002936627.1
Homo sapiens (human)	DEGS2	NP_996801.2
Rattus norvegicus (Norway rat)	Degs2	NP_001017457.1
Gallus gallus (chicken)	DEGS2	XP_421364.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011402	XP_317914.3
Eremothecium gossypii	AGOS_AGR025W	NP_986690.2
Schizosaccharomyces pombe (fission yeast)	dsd1	NP_596407.1
Neurospora crassa	NCU08927	XP_960609.1
Arabidopsis thaliana (thale cress)	DES-1-LIKE	NP_192402.1

Gene Ontology
Bibliography

Related articles in PubMed

Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil.
Lau CG, Marikawa Y
Molecular reproduction and development81(11)994-1008(2014 Nov)

Dihydroceramide-based response to hypoxia.
Devlin CM, Lahm T, Hubbard WC, Van Demark M, Wang KC, Wu X, Bielawska A, Obeid LM, Ivan M, Petrache I
The Journal of biological chemistry286(44)38069-78(2011 Nov)

Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.
Enomoto A, Omae F, Miyazaki M, Kozutsumi Y, Yubisui T, Suzuki A
The Biochemical journal397(2)289-95(2006 Jul)

Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.
Omae F, Miyazaki M, Enomoto A, Suzuki A
FEBS letters576(1-2)63-7(2004 Oct)

DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine.
Omae F, Miyazaki M, Enomoto A, Suzuki M, Suzuki Y, Suzuki A
The Biochemical journal379(Pt 3)687-95(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of DEGS1 or DEGS2 attenuates the DHC accumulation and increased cell proliferation during hypoxia
Title: Dihydroceramide-based response to hypoxia.

the hydroxylation of dihydroceramide: sphinganine C-4 requires complex formation between Des2 and mb5 via their membrane-spanning domains and electron transfer from NADH to the substrate via the reduction of mb5 by b5R
Title: Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.

DES2 is the dihydroceramide C-4 hydroxylase responsible for the biosynthesis of enriched phytosphingoglycolipids in the microvillous membranes of intestinal epithelial cells
Title: DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine.

The following Degs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Degs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11778	NM_001171002.1 Latest version!	Mus musculus delta(4)-desaturase, sphingolipid 2 (Degs2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu04010	NM_027299.5 Latest version!	Mus musculus delta(4)-desaturase, sphingolipid 2 (Degs2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Degs2 ( NM_001171002.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164473.1 Mus musculus delta(4)-desaturase, sphingolipid 2 (Degs2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11778
Clone ID Related Accession (Same CDS sequence)	NM_001171002.1
Accession Version	NM_001171002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Mus musculus(house mouse)
Product	sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008666.1, AK162979.1 and AC139568.5. Transcript Variant: This variant (2) has an alternate 3' exon, as compared to variant 1. The resulting isoform (2) is shorter and has a different C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK162979.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC 
CAGAGGCGCA AGGAGATGCT CGCCAAGTAC CCAGCCATCA AGGCCCTGAT GCGGCCAGAC 
CCTCACATCA AGTGGACTGT GTCGGGAATG GTACTGGTTC AGGTGCTGGC CTGCTGGTTA 
GTACGGGGAC TCTCTTGGCG ATGGCTGCTA TTTTGGGCCT ATGCCTTTGG TGGCTGCATC 
AACCACTCGC TGACACTGGC CATCCATGAC ATCTCGCACA ATACTGCCTT TGGCACAAGT 
TGTGTCTCCC GAAACCGCTG GTTTGCCATC TTTGCTAATC TGCCCATTGG CCTACCTTAC 
GCTACATCCT TCAAAAAGTA CCATGTGGAC CACCATCGTT ACCTGGGTGG AGACGGGCTG 
GATGTGGATA TTCCTACGAA CTTCGAGGGC TGGTTCTTCT GCACGCCGGC CCGCAAGCTG 
CTCTGGCTGG TGCTTCAGCC TTTCTTCTAT TCACTAAGGC CTCTCTGTGT GAACCCCAAG 
GTAGTGACAC GTATGGAGAT CCTCAACGCC CTGGTGCAGC TAGCATTTGA TGTCACCATC 
TTTGCCCTCT GGGGGATCAA GCCCATAGTC TACCTTCTAG GCAGCAGCCT CCTGGGTCTG 
GGCCTGCACC CTATCTCTGG TCACTTTGTG GCTGAGCATT ATATGTTCCT GAAGGGCCAC 
GAGACTTACT CCTACTATGG ACCCCTCAAT TGGATCACCT TCAATGTGGG TTACCACATG 
GAACACCACG ACTTCCCCAG TATCCCCGGT TACTACCTGC CACTGGATGA CTGGAACCCA 
GTGAGAAGAG ATGTGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164473.1
CDS	65..925
Translation	MGNSAARSDFEWVYSDQPHTQRRKEMLAKYPAIKALMRPDPHIKWTVSGMVLVQVLACWLVRGLSWRWLLFWAYAFGGCINHSLTLAIHDISHNTAFGTSCVSRNRWFAIFANLPIGLPYATSFKKYHVDHHRYLGGDGLDVDIPTNFEGWFFCTPARKLLWLVLQPFFYSLRPLCVNPKVVTRMEILNALVQLAFDVTIFALWGIKPIVYLLGSSLLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHMEHHDFPSIPGYYLPLDDWNPVRRDVL

Target ORF information:

RefSeq Version	NM_001171002.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus delta(4)-desaturase, sphingolipid 2 (Degs2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171002.1

ORF Insert Sequence:

ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC 
CAGAGGCGCA AGGAGATGCT CGCCAAGTAC CCAGCCATCA AGGCCCTGAT GCGGCCAGAC 
CCTCACATCA AGTGGACTGT GTCGGGAATG GTACTGGTTC AGGTGCTGGC CTGCTGGTTA 
GTACGGGGAC TCTCTTGGCG ATGGCTGCTA TTTTGGGCCT ATGCCTTTGG TGGCTGCATC 
AACCACTCGC TGACACTGGC CATCCATGAC ATCTCGCACA ATACTGCCTT TGGCACAAGT 
TGTGTCTCCC GAAACCGCTG GTTTGCCATC TTTGCTAATC TGCCCATTGG CCTACCTTAC 
GCTACATCCT TCAAAAAGTA CCATGTGGAC CACCATCGTT ACCTGGGTGG AGACGGGCTG 
GATGTGGATA TTCCTACGAA CTTCGAGGGC TGGTTCTTCT GCACGCCGGC CCGCAAGCTG 
CTCTGGCTGG TGCTTCAGCC TTTCTTCTAT TCACTAAGGC CTCTCTGTGT GAACCCCAAG 
GTAGTGACAC GTATGGAGAT CCTCAACGCC CTGGTGCAGC TAGCATTTGA TGTCACCATC 
TTTGCCCTCT GGGGGATCAA GCCCATAGTC TACCTTCTAG GCAGCAGCCT CCTGGGTCTG 
GGCCTGCACC CTATCTCTGG TCACTTTGTG GCTGAGCATT ATATGTTCCT GAAGGGCCAC 
GAGACTTACT CCTACTATGG ACCCCTCAAT TGGATCACCT TCAATGTGGG TTACCACATG 
GAACACCACG ACTTCCCCAG TATCCCCGGT TACTACCTGC CACTGGATGA CTGGAACCCA 
GTGAGAAGAG ATGTGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Degs2 ( NM_027299.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081575.2 Mus musculus delta(4)-desaturase, sphingolipid 2 (Degs2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04010
Clone ID Related Accession (Same CDS sequence)	NM_027299.5
Accession Version	NM_027299.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Mus musculus(house mouse)
Product	sphingolipid delta(4)-desaturase/C4-monooxygenase DES2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK008666.1, AK162979.1 and BU757432.1. On Jan 8, 2010 this sequence version replaced NM_027299.4. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC016427.1, AF466377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC 
CAGAGGCGCA AGGAGATGCT CGCCAAGTAC CCAGCCATCA AGGCCCTGAT GCGGCCAGAC 
CCTCACATCA AGTGGACTGT GTCGGGAATG GTACTGGTTC AGGTGCTGGC CTGCTGGTTA 
GTACGGGGAC TCTCTTGGCG ATGGCTGCTA TTTTGGGCCT ATGCCTTTGG TGGCTGCATC 
AACCACTCGC TGACACTGGC CATCCATGAC ATCTCGCACA ATACTGCCTT TGGCACAAGT 
TGTGTCTCCC GAAACCGCTG GTTTGCCATC TTTGCTAATC TGCCCATTGG CCTACCTTAC 
GCTACATCCT TCAAAAAGTA CCATGTGGAC CACCATCGTT ACCTGGGTGG AGACGGGCTG 
GATGTGGATA TTCCTACGAA CTTCGAGGGC TGGTTCTTCT GCACGCCGGC CCGCAAGCTG 
CTCTGGCTGG TGCTTCAGCC TTTCTTCTAT TCACTAAGGC CTCTCTGTGT GAACCCCAAG 
GTAGTGACAC GTATGGAGAT CCTCAACGCC CTGGTGCAGC TAGCATTTGA TGTCACCATC 
TTTGCCCTCT GGGGGATCAA GCCCATAGTC TACCTTCTAG GCAGCAGCCT CCTGGGTCTG 
GGCCTGCACC CTATCTCTGG TCACTTTGTG GCTGAGCATT ATATGTTCCT GAAGGGCCAC 
GAGACTTACT CCTACTATGG ACCCCTCAAT TGGATCACCT TCAATGTGGG TTACCACATG 
GAACACCACG ACTTCCCCAG TATCCCCGGT TACTACCTGC CACTGGTGCG GAAGATTGCA 
CCTGAATATT ATGACCACCT GCCGCAGCAC CACTCCTGGG TGAAAGTGCT CTGGGACTTC 
GTATTTGAGG ACTCTATGGG ACCCTACTCC AGGGTTAAGC GCAAGTGTAA GCTGGCGAAG 
GACCACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081575.2
CDS	65..1036
Translation	MGNSAARSDFEWVYSDQPHTQRRKEMLAKYPAIKALMRPDPHIKWTVSGMVLVQVLACWLVRGLSWRWLLFWAYAFGGCINHSLTLAIHDISHNTAFGTSCVSRNRWFAIFANLPIGLPYATSFKKYHVDHHRYLGGDGLDVDIPTNFEGWFFCTPARKLLWLVLQPFFYSLRPLCVNPKVVTRMEILNALVQLAFDVTIFALWGIKPIVYLLGSSLLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHMEHHDFPSIPGYYLPLVRKIAPEYYDHLPQHHSWVKVLWDFVFEDSMGPYSRVKRKCKLAKDHL

Target ORF information:

RefSeq Version	NM_027299.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus delta(4)-desaturase, sphingolipid 2 (Degs2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027299.5

ORF Insert Sequence:

ATGGGTAATA GCGCGGCCCG CAGCGACTTC GAGTGGGTCT ACAGCGACCA GCCGCACACC 
CAGAGGCGCA AGGAGATGCT CGCCAAGTAC CCAGCCATCA AGGCCCTGAT GCGGCCAGAC 
CCTCACATCA AGTGGACTGT GTCGGGAATG GTACTGGTTC AGGTGCTGGC CTGCTGGTTA 
GTACGGGGAC TCTCTTGGCG ATGGCTGCTA TTTTGGGCCT ATGCCTTTGG TGGCTGCATC 
AACCACTCGC TGACACTGGC CATCCATGAC ATCTCGCACA ATACTGCCTT TGGCACAAGT 
TGTGTCTCCC GAAACCGCTG GTTTGCCATC TTTGCTAATC TGCCCATTGG CCTACCTTAC 
GCTACATCCT TCAAAAAGTA CCATGTGGAC CACCATCGTT ACCTGGGTGG AGACGGGCTG 
GATGTGGATA TTCCTACGAA CTTCGAGGGC TGGTTCTTCT GCACGCCGGC CCGCAAGCTG 
CTCTGGCTGG TGCTTCAGCC TTTCTTCTAT TCACTAAGGC CTCTCTGTGT GAACCCCAAG 
GTAGTGACAC GTATGGAGAT CCTCAACGCC CTGGTGCAGC TAGCATTTGA TGTCACCATC 
TTTGCCCTCT GGGGGATCAA GCCCATAGTC TACCTTCTAG GCAGCAGCCT CCTGGGTCTG 
GGCCTGCACC CTATCTCTGG TCACTTTGTG GCTGAGCATT ATATGTTCCT GAAGGGCCAC 
GAGACTTACT CCTACTATGG ACCCCTCAAT TGGATCACCT TCAATGTGGG TTACCACATG 
GAACACCACG ACTTCCCCAG TATCCCCGGT TACTACCTGC CACTGGTGCG GAAGATTGCA 
CCTGAATATT ATGACCACCT GCCGCAGCAC CACTCCTGGG TGAAAGTGCT CTGGGACTTC 
GTATTTGAGG ACTCTATGGG ACCCTACTCC AGGGTTAAGC GCAAGTGTAA GCTGGCGAAG 
GACCACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Morphology-based mammalian stem cell tests reveal potential developmental toxicity of donepezil.
Molecular reproduction and development81(11)994-1008(2014 Nov)
Lau CG,Marikawa Y

Dihydroceramide-based response to hypoxia.
The Journal of biological chemistry286(44)38069-78(2011 Nov)
Devlin CM,Lahm T,Hubbard WC,Van Demark M,Wang KC,Wu X,Bielawska A,Obeid LM,Ivan M,Petrache I

Dihydroceramide:sphinganine C-4-hydroxylation requires Des2 hydroxylase and the membrane form of cytochrome b5.
The Biochemical journal397(2)289-95(2006 Jul)
Enomoto A,Omae F,Miyazaki M,Kozutsumi Y,Yubisui T,Suzuki A

Identification of an essential sequence for dihydroceramide C-4 hydroxylase activity of mouse DES2.
FEBS letters576(1-2)63-7(2004 Oct)
Omae F,Miyazaki M,Enomoto A,Suzuki A

DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine.
The Biochemical journal379(Pt 3)687-95(2004 May)
Omae F,Miyazaki M,Enomoto A,Suzuki M,Suzuki Y,Suzuki A