Cyp2j3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp2j3
Entrez Gene ID	313375
Full Name	cytochrome P450, family 2, subfamily j, polypeptide 3
Synonyms	Cyp2j9
General protein information	Preferred Names cytochrome P450, family 2, subfamily j, polypeptide 3 Names cytochrome P450 2J3 CYPIIJ3 cytochrome P450 monooxygenase cytochrome P450, 2j9 cytochrome P450, family 2, subfamily j, polypeptide 9
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q33
Summary	monooxygenase; responsible for the oxidation of endogenous arachidonic acid pools [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_175766.3	NP_786942.1	cytochrome P450 2J3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cyp2j9	NP_083255.1
Rattus norvegicus (Norway rat)	Cyp2j3	NP_786942.1
Gallus gallus (chicken)	CYP2J2	XP_422511.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004665	XP_318345.4
Gallus gallus (chicken)	LOC424676	XP_422509.2
Gallus gallus (chicken)	LOC424677	XP_004936904.1
Drosophila melanogaster (fruit fly)	phm	NP_573319.1

Gene Ontology
Bibliography

Related articles in PubMed

Glutamate affects the production of epoxyeicosanoids within the brain: The up-regulation of brain CYP2J through the MAPK-CREB signaling pathway.
Liu M, Zhu Q, Wu J, Yu X, Hu M, Xie X, Yang Z, Yang J, Feng YQ, Yue J
Toxicology38131-38(2017 04)

CYP2J3 gene delivery up-regulated adiponectin expression via reduced endoplasmic reticulum stress in adipocytes.
Xu X, Tu L, Feng W, Ma B, Li R, Zheng C, Li G, Wang DW
Endocrinology154(5)1743-53(2013 May)

Accumulation of cytochrome P450 induced by proteasome inhibition during cardiac ischemia.
Ishihara Y, Hamaguchi A, Sekine M, Hirakawa A, Shimamoto N
Archives of biochemistry and biophysics527(1)16-22(2012 Nov)

Upregulation of cytochrome P450 2J3/11,12-epoxyeicosatrienoic acid inhibits apoptosis in neonatal rat cardiomyocytes by a caspase-dependent pathway.
Wang HX, Zhang DM, Zeng XJ, Mu J, Yang H, Lu LQ, Zhang LK
Cytokine60(2)360-8(2012 Nov)

CYP2J3 gene delivery reduces insulin resistance via upregulation of eNOS in fructose-treated rats.
Xu X, Tu L, Wang L, Fang X, Wang DW
Cardiovascular diabetology10114(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increases in CYP2J3 mRNA levels were observed in the frontal cortex, hippocampus, cerebellum, and brain stem from rats that received the subcutaneous injection with a subneurotoxic dose of monosodium l-glutamate.
Title: Glutamate affects the production of epoxyeicosanoids within the brain: The up-regulation of brain CYP2J through the MAPK-CREB signaling pathway.

Beneficial roles of the CYP epoxygenase 2J3 and its metabolites epoxyeicosatrienoic acids on adiponectin expression and secretion.
Title: CYP2J3 gene delivery up-regulated adiponectin expression via reduced endoplasmic reticulum stress in adipocytes.

The protein levels of CYP 2c6, 2e1 and 2j3 increased in the ischemic region of the rat hearts, while the mRNA levels of CYPs were unchanged.
Title: Accumulation of cytochrome P450 induced by proteasome inhibition during cardiac ischemia.

Upregulation of CYP2J3/11,12-epoxyeicosatrienoic acid is involved in cardioprotection by inhibiting apoptosis via a caspase-dependent pathway.
Title: Upregulation of cytochrome P450 2J3/11,12-epoxyeicosatrienoic acid inhibits apoptosis in neonatal rat cardiomyocytes by a caspase-dependent pathway.

CYP2J3-derived epoxyeicosatrienoic acids may alleviate insulin resistance at least in part through upregulated eNOS expression.
Title: CYP2J3 gene delivery reduces insulin resistance via upregulation of eNOS in fructose-treated rats.

The following Cyp2j3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp2j3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12954	NM_175766.3 Latest version!	Rattus norvegicus cytochrome P450, family 2, subfamily j, polypeptide 3 (Cyp2j3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp2j3 ( NM_175766.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_786942.1 Rattus norvegicus cytochrome P450, family 2, subfamily j, polypeptide 3 (Cyp2j3), mRNA.

Price & Availability↑

CloneID	ORa12954
Clone ID Related Accession (Same CDS sequence)	NM_175766.3
Accession Version	NM_175766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 2J3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U39943.1, AAHX01038596.1 and AW527986.1. On Jul 19, 2012 this sequence version replaced NM_175766.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: U39943.1, BC089766.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTGTCA CAGCGGGTTC CCTACTAGGG GCCATTTGGA CCGTGCTCCA TCTCCGGATT 
TTGCTGCTGG CTGCTGTCAC CTTTCTGTTC CTGGCTGATT TCCTCAAACA CCGGCGCCCC 
AAGAACTACC CGCCAGGGCC TTGGCGCCTG CCCTTAGTGG GTTGCTTGTT CCATTTGGAT 
CCTAAACAGC CACACCTATC CCTTCAGCAG TTTGTGAAGA AATATGGGAA TGTTTTAAGC 
CTGGATTTTG CTAACATTCC TTCAGTGGTT GTAACTGGCA TGCCCTTAAT CAAAGAAATC 
TTTACTCAAA TGGAGCACAA TTTTTTGAAT CGCCCTGTCA CTCTTCTCCG AAAACATCTC 
TTTAACAAAA ATGGATTGAT CTTCTCCAGT GGCCAGACAT GGAAGGAGCA AAGGAGGTTT 
GCCCTGATGA CTCTGAGGAA CTTTGGACTA GGAAAGAAGA GCTTAGAGCA GCGAATACAA 
GAGGAAGCCT ACCACCTTGT GGAGGCCATA AAAGATGAGG GAGGACTGCC TTTTGACCCT 
CACTTCAATA TCAACAAAGC AGTCTCTAAT ATCATTTGCT CTGTCACCTT TGGGGAGCGC 
TTTGAGTACC ATGACAGTCA GTTTCAGGAG ATGCTGAGGC TACTGGATGA GGCCATGTGT 
TTGGAGTCAT CAATGATGTG CCAGCTCTAC AATATCTTTC CAAGGATACT TCAGTATCTT 
CCTGGATCAC ACCAAACACT TTTCAGCAAC TGGAGAAAAC TGAAGTTGTT TATTTCTGAT 
ATTATTAAGA ATCACCGGAG AGACTGGGAT CCAGATGAGC CAAGGGACTT CATTGATGCT 
TTCCTCAAGG AAATGGCAAA GTACCCAGAC AAGACTACTA CAAGTTTCAA TGAAGAAAAC 
CTCATCTGCA GTACTTTGGA TCTTTTCTTT GCTGGTACAG AGACAACATC CACTACACTG 
CGCTGGGCTC TGCTCTGCAT GGCCCTGTAC CCAGAAGTCC AAGAAAAAAT GCAGGCTGAG 
ATTGACAGAG TGATTGGCCA AGGAAGACAG CCAAACCTGG CAGACCGAGA CTCCATGCCC 
TACACCAACG CCGTCATTCA CGAGGTGCAG AGAATAGGCA ACATCATCCC CTTTAATGTT 
CCCAGGGAAG TGGCAGTTGA CACCTATTTG GCTGGATTTA ACTTGCCAAA GGGTACTATG 
ATTCTAACCA ACTTAACTGC ACTGCACAGG GACCCTAAAG AGTGGGCCAC CCCGGACACC 
TTCAACCCAG AGCACTTTTT GGAGAATGGA CAGTTTAAGA AGAGAGAATC CTTTCTGCCT 
TTCTCAATGG GAAAGCGAGC ATGTCTTGGA GAACAACTGG CCAGGTCCGA GCTGTTCATT 
TTTATCACTT CTCTTATCCA AAAATTTACC TTCAAGCCCC CGGTCAACGA GAAGCTTAGT 
CTGCAATTCA GAATGTCCGT CACCATTTCC CCAGTCAGTC ACCGCCTTTG CGCAATTCCT 
AGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786942.1
CDS	10..1518
Translation	MLVTAGSLLGAIWTVLHLRILLLAAVTFLFLADFLKHRRPKNYPPGPWRLPLVGCLFHLDPKQPHLSLQQFVKKYGNVLSLDFANIPSVVVTGMPLIKEIFTQMEHNFLNRPVTLLRKHLFNKNGLIFSSGQTWKEQRRFALMTLRNFGLGKKSLEQRIQEEAYHLVEAIKDEGGLPFDPHFNINKAVSNIICSVTFGERFEYHDSQFQEMLRLLDEAMCLESSMMCQLYNIFPRILQYLPGSHQTLFSNWRKLKLFISDIIKNHRRDWDPDEPRDFIDAFLKEMAKYPDKTTTSFNEENLICSTLDLFFAGTETTSTTLRWALLCMALYPEVQEKMQAEIDRVIGQGRQPNLADRDSMPYTNAVIHEVQRIGNIIPFNVPREVAVDTYLAGFNLPKGTMILTNLTALHRDPKEWATPDTFNPEHFLENGQFKKRESFLPFSMGKRACLGEQLARSELFIFITSLIQKFTFKPPVNEKLSLQFRMSVTISPVSHRLCAIPRL

Target ORF information:

RefSeq Version	NM_175766.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 2, subfamily j, polypeptide 3 (Cyp2j3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175766.3

ORF Insert Sequence:

ATGCTTGTCA CAGCGGGTTC CCTACTAGGG GCCATTTGGA CCGTGCTCCA TCTCCGGATT 
TTGCTGCTGG CTGCTGTCAC CTTTCTGTTC CTGGCTGATT TCCTCAAACA CCGGCGCCCC 
AAGAACTACC CGCCAGGGCC TTGGCGCCTG CCCTTAGTGG GTTGCTTGTT CCATTTGGAT 
CCTAAACAGC CACACCTATC CCTTCAGCAG TTTGTGAAGA AATATGGGAA TGTTTTAAGC 
CTGGATTTTG CTAACATTCC TTCAGTGGTT GTAACTGGCA TGCCCTTAAT CAAAGAAATC 
TTTACTCAAA TGGAGCACAA TTTTTTGAAT CGCCCTGTCA CTCTTCTCCG AAAACATCTC 
TTTAACAAAA ATGGATTGAT CTTCTCCAGT GGCCAGACAT GGAAGGAGCA AAGGAGGTTT 
GCCCTGATGA CTCTGAGGAA CTTTGGACTA GGAAAGAAGA GCTTAGAGCA GCGAATACAA 
GAGGAAGCCT ACCACCTTGT GGAGGCCATA AAAGATGAGG GAGGACTGCC TTTTGACCCT 
CACTTCAATA TCAACAAAGC AGTCTCTAAT ATCATTTGCT CTGTCACCTT TGGGGAGCGC 
TTTGAGTACC ATGACAGTCA GTTTCAGGAG ATGCTGAGGC TACTGGATGA GGCCATGTGT 
TTGGAGTCAT CAATGATGTG CCAGCTCTAC AATATCTTTC CAAGGATACT TCAGTATCTT 
CCTGGATCAC ACCAAACACT TTTCAGCAAC TGGAGAAAAC TGAAGTTGTT TATTTCTGAT 
ATTATTAAGA ATCACCGGAG AGACTGGGAT CCAGATGAGC CAAGGGACTT CATTGATGCT 
TTCCTCAAGG AAATGGCAAA GTACCCAGAC AAGACTACTA CAAGTTTCAA TGAAGAAAAC 
CTCATCTGCA GTACTTTGGA TCTTTTCTTT GCTGGTACAG AGACAACATC CACTACACTG 
CGCTGGGCTC TGCTCTGCAT GGCCCTGTAC CCAGAAGTCC AAGAAAAAAT GCAGGCTGAG 
ATTGACAGAG TGATTGGCCA AGGAAGACAG CCAAACCTGG CAGACCGAGA CTCCATGCCC 
TACACCAACG CCGTCATTCA CGAGGTGCAG AGAATAGGCA ACATCATCCC CTTTAATGTT 
CCCAGGGAAG TGGCAGTTGA CACCTATTTG GCTGGATTTA ACTTGCCAAA GGGTACTATG 
ATTCTAACCA ACTTAACTGC ACTGCACAGG GACCCTAAAG AGTGGGCCAC CCCGGACACC 
TTCAACCCAG AGCACTTTTT GGAGAATGGA CAGTTTAAGA AGAGAGAATC CTTTCTGCCT 
TTCTCAATGG GAAAGCGAGC ATGTCTTGGA GAACAACTGG CCAGGTCCGA GCTGTTCATT 
TTTATCACTT CTCTTATCCA AAAATTTACC TTCAAGCCCC CGGTCAACGA GAAGCTTAGT 
CTGCAATTCA GAATGTCCGT CACCATTTCC CCAGTCAGTC ACCGCCTTTG CGCAATTCCT 
AGGCTGTGA