Rap1gds1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rap1gds1
Entrez Gene ID	310909
Full Name	Rap1 GTPase-GDP dissociation stimulator 1
General protein information	Preferred Names Rap1 GTPase-GDP dissociation stimulator 1 Names rap1 GTPase-GDP dissociation stimulator 1 RAP1, GTP-GDP dissociation stimulator 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107728.1	NP_001101198.1	rap1 GTPase-GDP dissociation stimulator 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAP1GDS1	NP_001093896.1
Pan troglodytes (chimpanzee)	RAP1GDS1	XP_001165462.1
Bos taurus (cattle)	RAP1GDS1	NP_777091.2
Rattus norvegicus (Norway rat)	Rap1gds1	NP_001101198.1
Gallus gallus (chicken)	RAP1GDS1	NP_001026321.1
Drosophila melanogaster (fruit fly)	vimar	NP_477305.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005262	XP_314182.3
	RAP1GDS1	XP_001104606.1
Canis lupus familiaris (dog)	RAP1GDS1	XP_005639273.1
Mus musculus (house mouse)	Rap1gds1	NP_001273688.1
Danio rerio (zebrafish)	rap1gds1	XP_002667044.1
Xenopus tropicalis (tropical clawed frog)	LOC100486041	XP_002936785.2

Gene Ontology
Bibliography

Related articles in PubMed

Transglutaminase 2 contributes to apoptosis induction in Jurkat T cells by modulating Ca2+ homeostasis via cross-linking RAP1GDS1.
Hsieh YF, Liu GY, Lee YJ, Yang JJ, S?ndor K, Sarang Z, Bononi A, Pinton P, Tretter L, Szondy Z, Tsay GJ
PloS one8(12)e81516(2013)

SmgGDS antagonizes BPGAP1-induced Ras/ERK activation and neuritogenesis in PC12 cell differentiation.
Ravichandran A, Low BC
Molecular biology of the cell24(2)145-56(2013 Jan)

Anaplastic lymphoma kinase activates the small GTPase Rap1 via the Rap1-specific GEF C3G in both neuroblastoma and PC12 cells.
Sch?nherr C, Yang HL, Vigny M, Palmer RH, Hallberg B
Oncogene29(19)2817-30(2010 May)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Identification and characterization of the unique guanine nucleotide exchange factor, SmgGDS, in vascular smooth muscle cells.
Thill R, Campbell WB, Williams CL
Journal of cellular biochemistry104(5)1760-70(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SmgGDS, a novel Ras regulator, suppresses Ras-BPGAP1-induced Ras/ERK activation.
Title: SmgGDS antagonizes BPGAP1-induced Ras/ERK activation and neuritogenesis in PC12 cell differentiation.

ALK activation of Rap1 via the Rap1-specific GEF C3Gmay contribute to cell proliferation and oncogenesis of neuroblastoma.
Title: Anaplastic lymphoma kinase activates the small GTPase Rap1 via the Rap1-specific GEF C3G in both neuroblastoma and PC12 cells.

These results identify SmgGDS as a novel regulator of myosin organization and contraction in vascular smooth muscle cells.
Title: Identification and characterization of the unique guanine nucleotide exchange factor, SmgGDS, in vascular smooth muscle cells.

betaPak-interacting exchange factor-mediated Rac1 activation requires smgGDS guanine nucleotide exchange factor in basic fibroblast growth factor-induced neurite outgrowth
Title: betaPak-interacting exchange factor-mediated Rac1 activation requires smgGDS guanine nucleotide exchange factor in basic fibroblast growth factor-induced neurite outgrowth.

The following Rap1gds1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rap1gds1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05765	NM_001107728.1 Latest version!	Rattus norvegicus Rap1 GTPase-GDP dissociation stimulator 1 (Rap1gds1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rap1gds1 ( NM_001107728.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101198.1 Rattus norvegicus Rap1 GTPase-GDP dissociation stimulator 1 (Rap1gds1), mRNA.

Price & Availability↑

CloneID	ORa05765
Clone ID Related Accession (Same CDS sequence)	NM_001107728.1
Accession Version	NM_001107728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	rap1 GTPase-GDP dissociation stimulator 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473952.1. On or before Oct 4, 2007 this sequence version replaced XM_227749.3, XM_001078472.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGAATCC CATGTGTAGA TGCCGGATTG ATTTCACCAC TGGTACAGCT GTTAAATAGC 
AAAGACCAGG AAGTGCTGCT TCAAACTGGC AGGGCATTAG GAAACATATG CTATGATAGC 
CATTCCCTTC AGGCTCAGCT AATCAATATG GGTGTCATCC CGACCTTAGT GAAGTTACTG 
GGCATCCACT GCCACAACGC TGCTCTGACA GAGATGTGCC TCGTGGCATT TGGGAACTTA 
GCAGAACTCG AGTCGAGTAA AGAACAGTTT GCTAGCACAA ACATTGCCGA AGAACTGGTA 
AAACTATTCA AGAAACAAAT AGAGCATGAT AAAAGAGAAA TGATTTTCGA AGTTCTAGCT 
CCATTGGCAG AAAATGACGC CATTAAACTG CAGCTGGTTG AAGCAGGTTT GGTCGAGTGC 
CTCCTAGAGA TTGTTCAGCA GAAGGTGGAC AGTACCAAAG AGGATGACCT TGCTGAGCTC 
AAAACTGCTT CAGACCTCAT GGTTTTATTA CTTCTTGGAG ATGAATCCAT GCAGAAACTT 
TTTGAAGGAG GAAAAGGCAG TGTGTTCCAA AGGGTGCTCT CTTGGATTCC ATCGAATAGC 
CAGCAGCTGC AGCTTGCTGG GGCATTGGCC ATTGCAAATT TTGCCAGGAA TGATGGGAAT 
TGTATTCATA TGGTAGACAA TGGGATTGTA GAAAAACTCA TGGATTTGCT GGACAGACAC 
GTAGAAGACG GGAACGTGAC GGTACAGCAT GCAGCACTGA GCGCCCTCAG GAACCTAGCC 
ATTCCAGTTG TAAATAAAGC AAAGATGTTA TCAGCCGGGG TCACAGAGAC GGTTTTGAAG 
TTCCTTAAAT CTGAAATGCC CCCAGTTCAG TTCAAACTCC TGGGAACGCT AAGAATGTTA 
ATAGATGCAC AAGCAGAAGC TGCTGAGCAA CTGGGAAAGA ACGCTAAGTT GGTGGAGCGC 
TTGGTTGAGT GGTGTGAAGC CAAAGATCAT GCCGGTGTGA TGGGCGAGTC AAACAGACTG 
CTGTCCGCTC TCATACGACA CAGTAAATCA AAAGATGTAA TTAAAACCAT TGTACAGAGC 
GGTGGCATCA AGCATCTAGT TACCATGGCA ACTAGTGAGC ATGTGATAAT GCAGAATGAG 
GCTCTCGTCG CGTTGGCACT AATTGCAGCT TTGGAATTGG GTCCTGCGGA GAAAGATCTC 
GCAAGTGCTC AGCTTGTCCA GATCCTACAC CGACTGCTAG CAGATGAGAG AAGTGCCCCG 
GAAATCAAGT ATAACTCCAT GGTTCTGATC TGTGCTCTCA TGGGCTCTGA AGCACTACAC 
AAGGAAGTAC AAGACTTGGC CTTCCTAGAT GTTGTATCCA AACTTCGAAG TCATGAGAAC 
AAAAGTGTTG CCCAACAGGC CTCGCTCACA GAGCAGAGAC TTACAGTGCA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101198.1
CDS	243..1679
Translation	MRIPCVDAGLISPLVQLLNSKDQEVLLQTGRALGNICYDSHSLQAQLINMGVIPTLVKLLGIHCHNAALTEMCLVAFGNLAELESSKEQFASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQKVDSTKEDDLAELKTASDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNSQQLQLAGALAIANFARNDGNCIHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPVVNKAKMLSAGVTETVLKFLKSEMPPVQFKLLGTLRMLIDAQAEAAEQLGKNAKLVERLVEWCEAKDHAGVMGESNRLLSALIRHSKSKDVIKTIVQSGGIKHLVTMATSEHVIMQNEALVALALIAALELGPAEKDLASAQLVQILHRLLADERSAPEIKYNSMVLICALMGSEALHKEVQDLAFLDVVSKLRSHENKSVAQQASLTEQRLTVQS

Target ORF information:

RefSeq Version	NM_001107728.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap1 GTPase-GDP dissociation stimulator 1 (Rap1gds1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107728.1

ORF Insert Sequence:

ATGCGAATCC CATGTGTAGA TGCCGGATTG ATTTCACCAC TGGTACAGCT GTTAAATAGC 
AAAGACCAGG AAGTGCTGCT TCAAACTGGC AGGGCATTAG GAAACATATG CTATGATAGC 
CATTCCCTTC AGGCTCAGCT AATCAATATG GGTGTCATCC CGACCTTAGT GAAGTTACTG 
GGCATCCACT GCCACAACGC TGCTCTGACA GAGATGTGCC TCGTGGCATT TGGGAACTTA 
GCAGAACTCG AGTCGAGTAA AGAACAGTTT GCTAGCACAA ACATTGCCGA AGAACTGGTA 
AAACTATTCA AGAAACAAAT AGAGCATGAT AAAAGAGAAA TGATTTTCGA AGTTCTAGCT 
CCATTGGCAG AAAATGACGC CATTAAACTG CAGCTGGTTG AAGCAGGTTT GGTCGAGTGC 
CTCCTAGAGA TTGTTCAGCA GAAGGTGGAC AGTACCAAAG AGGATGACCT TGCTGAGCTC 
AAAACTGCTT CAGACCTCAT GGTTTTATTA CTTCTTGGAG ATGAATCCAT GCAGAAACTT 
TTTGAAGGAG GAAAAGGCAG TGTGTTCCAA AGGGTGCTCT CTTGGATTCC ATCGAATAGC 
CAGCAGCTGC AGCTTGCTGG GGCATTGGCC ATTGCAAATT TTGCCAGGAA TGATGGGAAT 
TGTATTCATA TGGTAGACAA TGGGATTGTA GAAAAACTCA TGGATTTGCT GGACAGACAC 
GTAGAAGACG GGAACGTGAC GGTACAGCAT GCAGCACTGA GCGCCCTCAG GAACCTAGCC 
ATTCCAGTTG TAAATAAAGC AAAGATGTTA TCAGCCGGGG TCACAGAGAC GGTTTTGAAG 
TTCCTTAAAT CTGAAATGCC CCCAGTTCAG TTCAAACTCC TGGGAACGCT AAGAATGTTA 
ATAGATGCAC AAGCAGAAGC TGCTGAGCAA CTGGGAAAGA ACGCTAAGTT GGTGGAGCGC 
TTGGTTGAGT GGTGTGAAGC CAAAGATCAT GCCGGTGTGA TGGGCGAGTC AAACAGACTG 
CTGTCCGCTC TCATACGACA CAGTAAATCA AAAGATGTAA TTAAAACCAT TGTACAGAGC 
GGTGGCATCA AGCATCTAGT TACCATGGCA ACTAGTGAGC ATGTGATAAT GCAGAATGAG 
GCTCTCGTCG CGTTGGCACT AATTGCAGCT TTGGAATTGG GTCCTGCGGA GAAAGATCTC 
GCAAGTGCTC AGCTTGTCCA GATCCTACAC CGACTGCTAG CAGATGAGAG AAGTGCCCCG 
GAAATCAAGT ATAACTCCAT GGTTCTGATC TGTGCTCTCA TGGGCTCTGA AGCACTACAC 
AAGGAAGTAC AAGACTTGGC CTTCCTAGAT GTTGTATCCA AACTTCGAAG TCATGAGAAC 
AAAAGTGTTG CCCAACAGGC CTCGCTCACA GAGCAGAGAC TTACAGTGCA AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transglutaminase 2 contributes to apoptosis induction in Jurkat T cells by modulating Ca2+ homeostasis via cross-linking RAP1GDS1.
PloS one8(12)e81516(2013)
Hsieh YF,Liu GY,Lee YJ,Yang JJ,S?ndor K,Sarang Z,Bononi A,Pinton P,Tretter L,Szondy Z,Tsay GJ

SmgGDS antagonizes BPGAP1-induced Ras/ERK activation and neuritogenesis in PC12 cell differentiation.
Molecular biology of the cell24(2)145-56(2013 Jan)
Ravichandran A,Low BC

Anaplastic lymphoma kinase activates the small GTPase Rap1 via the Rap1-specific GEF C3G in both neuroblastoma and PC12 cells.
Oncogene29(19)2817-30(2010 May)
Sch?nherr C,Yang HL,Vigny M,Palmer RH,Hallberg B

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Identification and characterization of the unique guanine nucleotide exchange factor, SmgGDS, in vascular smooth muscle cells.
Journal of cellular biochemistry104(5)1760-70(2008 Aug)
Thill R,Campbell WB,Williams CL