Tnrc6a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnrc6a
Entrez Gene ID	308971
Full Name	trinucleotide repeat containing 6a
Synonyms	Tnrc6
General protein information	Preferred Names trinucleotide repeat containing 6a Names trinucleotide repeat-containing gene 6A protein trinucleotide repeat containing 6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_008759748.2	XP_008757970.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_008759749.2	XP_008757971.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_017589208.1	XP_017444697.1	trinucleotide repeat-containing gene 6A protein isoform X4
XM_008759747.2	XP_008757969.1	trinucleotide repeat-containing gene 6A protein isoform X1
XM_017589209.1	XP_017444698.1	trinucleotide repeat-containing gene 6A protein isoform X5
NM_001107549.1	NP_001101019.1	trinucleotide repeat-containing gene 6A protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	TNRC6A	NP_055309.2
	TNRC6A	XP_001098013.2
Bos taurus (cattle)	TNRC6A	XP_005224864.1
Gallus gallus (chicken)	TNRC6A	XP_414871.4
Xenopus tropicalis (tropical clawed frog)	tnrc6a	XP_002932511.2
Canis lupus familiaris (dog)	TNRC6A	XP_005621456.1
Mus musculus (house mouse)	Tnrc6a	NP_659174.3
Rattus norvegicus (Norway rat)	Tnrc6a	NP_001101019.1
Pan troglodytes (chimpanzee)	TNRC6A	XP_510885.3

Gene Ontology
Bibliography

Related articles in PubMed

The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets.
Huntzinger E, Kuzuoglu-?zt?rk D, Braun JE, Eulalio A, Wohlbold L, Izaurralde E
Nucleic acids research41(2)978-94(2013 Jan)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.
James V, Zhang Y, Foxler DE, de Moor CH, Kong YW, Webb TM, Self TJ, Feng Y, Lagos D, Chu CY, Rana TM, Morley SJ, Longmore GD, Bushell M, Sharp TV
Proceedings of the National Academy of Sciences of the United States of America107(28)12499-504(2010 Jul)

Argonaute proteins at a glance.
Ender C, Meister G
Journal of cell science123(Pt 11)1819-23(2010 Jun)

Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system.
Wakiyama M, Takimoto K, Ohara O, Yokoyama S
Genes & development21(15)1857-62(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tnrc6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnrc6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa41414	NM_001107549.1 Latest version!	Rattus norvegicus trinucleotide repeat containing adaptor 6A (Tnrc6a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa23390	XM_017589209.1 Latest version!	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa46682	XM_008759747.2 Latest version!	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa46683	XM_008759748.2 Latest version!	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa46684	XM_008759749.2 Latest version!	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa56612	XM_017589208.1 Latest version!	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnrc6a ( NM_001107549.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101019.1 Rattus norvegicus trinucleotide repeat containing adaptor 6A (Tnrc6a), mRNA.

Price & Availability↑

CloneID	ORa41414
Clone ID Related Accession (Same CDS sequence)	NM_001107549.1
Accession Version	NM_001107549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Rattus norvegicus(Norway rat)
Product	trinucleotide repeat-containing gene 6A protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473956.2. On or before Oct 4, 2007 this sequence version replaced XM_001079613.1, XM_219297.4.

ATGGAAGAAA AGAAAAGGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CTAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CCGATAACGG CACTTCCACA GCTACCAGCA CTAATAATAA TGCCAAGCGA 
GCTACAGCCA GCAATCAGCA GCCGCCGCCG CCGCCGCAGC AGCCGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAACAAGA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCACAAG CCTTGCCTCG GTATCCTCGT GAAGTACCTC CACGATTTCG CCACCAGGAA 
CACAAACAGC TTCTAAAGAG GGGTCAGCAT TTTCCAGTCA TAGCAGCAAA CTTGGGATCT 
GCTGTTAAGG TGTTAAACAG CCAATCAGAA AGCAGTGCTG TAACAAATCA ACAGCCACAA 
AATAACGGAG AGGTGCAGAA CAGCAAAAGC CAGTCAGATA TAAATCATAA TACTTCAGGA 
TCCCATTATG AAAATTGCCA GCGGGGACCT GTGTCTTCTA CAAGTGACTG TAGCACAAGC 
TGTAAGAATG CTGTAAACGA CTTGTTGGAA AAAGAAGCAT GGCCCTCAGC CCCTGGCAGT 
GATCCTGAGT TGGCTTCAGA ATGTATAGAT GCTGATTCTG CCTCCAATTC TGAGTCAGAG 
AGAAACATCA CTGTCATGGC TTCAGGGAAC ACAGGTGGTG AGAAAGATGG CCTTCGGAAC 
AGCACTGGAC TCGGTCCCCA AAGCAAATTT GTGGTTGGTA GCAGCAGCAA TAATGTGGGT 
CATGGAAGTA GTACTGGGCC ATGGGGCTTT CCCCATGGAG CCATAATAAG CACATGTCAG 
GTCTCTGTGG ATGCTCCTGA AAGCAAACCA GAAAGTAGTA ACAGTAGAAT GAATGCTTGG 
GGCACTGTAA GTTCTTCATC AAATGGAGGG TTAAATCCAA GCACTTTGAA TTCAGCTAGC 
AACCATGGTG CCTGGCCAGT ATTAGAAAAC AACGGACTTG CCCTAAAAGG GCCTGTAGGG 
AGTGGGAATT CTGGCATCAA TATTCAGTGT AGTACCATAG GCCAGATGCC TAACAATCAG 
AGTATCAACT CTAAAGTCAG TGGCTCTTCT ACCCATGGTA CCTGGGGTAG CCTTCAGGAA 
ACTTGTGAGC CTGAAGTAAG TGGTTCACAG AAGGTTTCAT TCAGTGGTCA ACCTCAGAAT 
ATCACCACTG AAACGACTGG ACCAAATAAC ACTACTAACT TTATGACCTC TAGTTTACCA 
AACTCCGGTT CAGTACAAAA TAATGAACTG CCTACTAGTA ATCCAGGGGC CTGGCGTGTG 
AGCACAATGA ATCATCCTCA GATACAGGCT CCGTCAGTTC TGAATGGCAC TTCCCTTTCT 
CACCTTAGTA ATGGAGAGTC AAAAACTGGA GGCTCCTACG GTACTACATG GGGTGCCTAT 
GGTTCTAATT ACTCTGGCGA CAAATGTGCA GGCCCTAATG GCCAAGCCAA TGGTGACACT 
GTGAATGCAA CTCTAATGCA GCCTGGCATA AATGGGCCTA TGGGCACTAA CTTTCAAGTT 
AGTACAAATA AAGGGGGAGG TGTATGGGAG CCTGGGACAG TGAATTCCCA GAGTTCACCA 
TGGGGAAGTG GAAATGGTGC AAATTCTGGA GGAAGTCGAA GAGGATGGGG AAGTCCTGCA 
CAGAACACTG GCACTAGTCT GTCCAGTGTT GAGTGGAACA AACTGCCTAG CAACCAGCAT 
TCCAATGACA GTGCAAATGG CAATGGTAGT AAGAAACTTA CAAATGGATG GAAATCTACT 
GAGGAAGATG ATCAGGGTTC TGGCACATCT CAGACGAATG AGCAAAACAG TGTGTGGGCC 
AAAGCAGGAG GCACAGTGGA GAGTGATGGT AGTGCAGAGA GCACTGGACG CCTTGAAGAA 
AAAGTAACCG GGGAAAGCCA GAGTAGAGAT AGAAGAAAAA TTGATCAGCA CACATTACTC 
CAAAGCATTG TAAACAGAAC TGACTTAGAT CCACGTGTCC TATCCAATTC TGGGTGGGGA 
CAGACTCCTA TTAAGCAGAA TACTGCCTGG GATACAGAGA CATCACCAAG AGGGGAAAGA 
AAGACTGACA ATGGGACAGA GGCCTGGGGA AGCTCTGCAA CACAGACTTT TAACTCAGGG 
GCATGTACAG ATAAGACTAG CCCTAATAGT AATGATACCT CATCTGTATC AGGGTGGGGT 
GATCCCAAAC CTACTCTGAG GTGGGGAGAT TCCAAAGGCT CAAACTGCCA GGGGGGGTGG 
GAAGATGATT CTGCTGCTAC AGGAATGATC AAGAGCAATC AGTGGGGGCA TTGCAAAGAA 
GACAAGTCTG CATGGAATGA TTCGCAAAAG AACAAACAAG GCTGGGGCGA TGGACAAAAG 
TCAAGCCAAG GTTGGTCCGT TTCTGCCAGT GATAACTGGG GAGAATCTTC CAGGAGTAAC 
CATTGGGGTG AGGCTAATAA GAAATCCAGC TCAGGAGGCA GTGACAGCGA CAGGTCCATT 
TCTGGTTGGA ACGAACTTGG TAAAACTAGT TCTTTTACTT GGGGAAATAA TATAAATCCA 
AATAACTCAT CAGGATGGGA TGAATCTTCT AAACCTAACT CTTCCCAGGG ATGGGGAGAC 
CCTCCAAAGT GTAATCAGTC CCTAGGTTGG GGAGATTCAT CAAAACCAGT TAGTTCTCCA 
GATTGGAACA AGCAACAAGA CATTGTTGGA TCGTGGGGAA TCCCACCAGC CACCAGCAAG 
CCTCCTGGTA CAGGCTGGCT TGGGGGACCT ATTCCTGCTC CAGCAAAGGA GGAAGAACCC 
ACAGGCTGGG AGGAACCATC CCCAGAGTCT ATACGACGAA AAATGGAGAT CGATGATGGA 
ACTTCAGCTT GGGGAGATCC AAGCAAATAC AACTACAAAA ATGTGAACAT GTGGAATAAA 
AACATCCCAG AAGGCAGCAG CCGCTCAGAC CAGCAAGCAC AGATGCACCG GTTGTTGCCG 
GCTGCAAGTG CCATCTCAAG CAAGGAGACC AGCAGTGGCT CTGGCTGGGG TGAGCCTTGG 
GCAGAGCCTT CTACTCCAGC CACGACTGTG GATAATGGTA CTTCAGCCTG GGGCAAGCCC 
ATAGACAGTG GTCCCAGCTG GGGAGAACCC ATTACTGCAG CATCCAGTGC ATCCACGTGG 
GGCTCCAACT CTGTTGGTCC ACAATCATTA AGCAAATCTG GACCCAAATC TATGCAAGAT 
GGCTGGTGTG GTGAGGACAC GCCATTGCCT GGAAGTCGCC CCACTGGCTG GGAAGAGGAG 
GAGGATGTAG AGATTGGGAT GTGGAACAGT AGCTCATCTC AAGAGCTTAA CTCATCCTTA 
AACTGGCCAC CATATACCAA GAAAATGTCA TCCAAGGGTC TGAGTGGCAA AAAAAGGAGA 
AGGGAAAGGG GAATGATGAA AGGCGGAAAC AAACAAGAGG ACGCGTGGAT AAACCCATTC 
GTTAAGCAGT TTTCAAATAT CAGTTTTTCA AGAGACTCAC CAGAAGAAAA TGTACAGAGC 
AATAAGATGG ATCTTTCTGG AGGAATGTTA CAGGACAAGC GGATGGAGAT TGATAAGCAT 
AGCCTGAATA TTGGTGACTA CAATCGAGCG GTCGGGAAAG GCCCTGGGTC TCGGCCTCAG 
ATTTCCAAAG AGTCTTCCAT GGAGCGCAAT CCTTACTTTG ATAAGGATGG CATTGTAGCA 
GATGAATCCC AAAACATGCA GTTTATGTCC AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT 
AATGCACTAC CTAACCAGGC CCTTGGCTCC ATAGCAGGGC TGGGTACGCA AAACTTGAAT 
TCTGTTAGAC AGAATGGCAA TCCCAGTATG TTTGGTGTTG GAAACACAGC AGCACAACCC 
CGGGGCATGC AGCAGCCTCC AGCACAACCT CTCAGTTCAT CTCAGCCTAA TCTTCGTGCT 
CAAGTGCCTC CTCCATTACT CTCCCCTCAG GTTCCAGTTT CATTGCTGAA GTATGCACCA 
AACAACGGTG GCCTGAATCC GCTCTTTGGC CCTCAACAGG TAGCCATGCT GAACCAGCTA 
TCCCAACTAA ACCAGCTTTC TCAGATCTCC CAGTTACAGC GATTGTTAGC GCAGCAGCAG 
AGGGCACAGA GTCAGAGAAG CGTGCCTTCT GCTAACCGAC AGCAGCAAGA CCAGCAGGGT 
CGACCTCTTA GTGTACAGCA GCAGATGATG CAACAGTCTC GTCAGCTTGA TCCAAGCCTG 
CTGGTGAAGC AGCAGACTCC GCCTTCTCAG CAGCCGCTCC ATCAGCCAGC CATGAAGTCC 
TTCCTTGACA ATGTCATGCC CCACACTACA CCCGAGCTAC AGAAAGGGCC ATCGCCAGTA 
AACGCTTTCA GCAGCTTCCC TATAGGCTTG AACTCAAACT TGAATGTAAA TATGGATATG 
AACAGTATTA AAGAGCCACA GTCACGACTA AGGAAGTGGA CAACAGTGGA CAGCATGTCT 
GTGAACACGT CTTTGGATCA AAACTCCAGC AAACATGGTG CTATTTCAAG TGGTTTTAGG 
CTGGAAGAGT CTCCATTTGT TCCCTATGAC TTTATGAATA GCAGTACTTC ACCAGCCAGT 
CCTCCAGGTT CAATAGGAGA TGGCTGGCCA CGTGCCAAAT CGCCTAACGG CTCTAGCAGT 
GTTAACTGGC CACCAGAATT TCGCCCTGGT GAACCATGGA AAGGTTATCC AAACATTGAC 
CCTGAAACTG ACCCTTACGT CACTCCTGGC AGTGTCATAA ACAGTCTTTC AATTAATACT 
GTGCGGGAAG TTGACCACCT CAGGGACAGG AACAGTGGGT CATCCTCATC CTTGAACACC 
ACGCTGCCTT CAACTAGTGC CTGGTCATCC ATTCGTGCCT CCAACTACAA TGTTCCCCTC 
AGCAGTACAG CACAAAGCAC TTCAGCCAGA AATAGTGATT CCAAATTGAC GTGGTCTCCT 
GGTTCAGTTA CAAACACCTC TCTGGCTCAT GAGCTGTGGA AAGTCCCTTT ACCACCTAAA 
AACATCACTG CTCCGTCCCG CCCACCTCCG GGGCTGACTG GTCAGAAGCC ACCCTTGTCT 
ACGTGGGATA ATTCTCCCCT TCGTGTAGGT GGAGGATGGG GAAATTCTGA CGCCAGATAT 
ACCCCAGGCT CCAGCTGGGG TGAGAGCAGC TCAGGGAGAA TAACCAATTG GCTTGTTCTG 
AAGAACCTCA CACCTCAGAT TGACGGCTCC ACTCTGCGCA CCCTGTGCAT GCAGCATGGC 
CCACTGATTA CATTCCATCT GAACCTCCCA CATGGAAATG CTCTGGTCCG TTACAGTTCA 
AAGGAAGAGG TAGTGAAGGC ACAAAAGTCT CTGCACATGT GTGTGCTGGG GAACACTACT 
ATTCTCGCTG AGTTTGCCAG TGAAGAGGAG ATCAGTCGCT TCTTTGCACA AAGTCAGTCT 
CTGACCCCTT CTCCTGGCTG GCAGTCTCTC GGGTCCAGCC AGAGCCGGCT GGGCTCCCTC 
GACTGTTCCC ACTCATTCTC CAGCCGGACT GATCTCAATC ACTGGAATGG TGCTGGGCTG 
TCGGGAACTA ACTGTGGAGA CCTTCACGGC ACTTCCCTCT GGGGGACCCC ACATTATTCC 
ACAAGCCTGT GGGGTCCCCC AAGCAGCAGT GACCCCCGGG GAATTAGCAG CCCATCCCCC 
ATTAATGCTT TTCTTTCTGT TGACCACCTG GCTTTTATTT TTATTTTTTT TTTGTTTTTT 
TGTTTTTTTG TTTTTTTTTT TTTTTGCAAA ACCGTTTTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101019.1
CDS	103..5967
Translation	MEEKKRKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSDNGTSTATSTNNNAKRATASNQQPPPPPQQPQQQQQQQQQQQEQQQQQQQQQQQQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSAVTNQQPQNNGEVQNSKSQSDINHNTSGSHYENCQRGPVSSTSDCSTSCKNAVNDLLEKEAWPSAPGSDPELASECIDADSASNSESERNITVMASGNTGGEKDGLRNSTGLGPQSKFVVGSSSNNVGHGSSTGPWGFPHGAIISTCQVSVDAPESKPESSNSRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGNSGINIQCSTIGQMPNNQSINSKVSGSSTHGTWGSLQETCEPEVSGSQKVSFSGQPQNITTETTGPNNTTNFMTSSLPNSGSVQNNELPTSNPGAWRVSTMNHPQIQAPSVLNGTSLSHLSNGESKTGGSYGTTWGAYGSNYSGDKCAGPNGQANGDTVNATLMQPGINGPMGTNFQVSTNKGGGVWEPGTVNSQSSPWGSGNGANSGGSRRGWGSPAQNTGTSLSSVEWNKLPSNQHSNDSANGNGSKKLTNGWKSTEEDDQGSGTSQTNEQNSVWAKAGGTVESDGSAESTGRLEEKVTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPNSNDTSSVSGWGDPKPTLRWGDSKGSNCQGGWEDDSAATGMIKSNQWGHCKEDKSAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGESSRSNHWGEANKKSSSGGSDSDRSISGWNELGKTSSFTWGNNINPNNSSGWDESSKPNSSQGWGDPPKCNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATSKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNIPEGSSRSDQQAQMHRLLPAASAISSKETSSGSGWGEPWAEPSTPATTVDNGTSAWGKPIDSGPSWGEPITAASSASTWGSNSVGPQSLSKSGPKSMQDGWCGEDTPLPGSRPTGWEEEEDVEIGMWNSSSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEDAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRAVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNNALPNQALGSIAGLGTQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSANRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQPLHQPAMKSFLDNVMPHTTPELQKGPSPVNAFSSFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSMSVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINSLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLAFIFIFFLFFCFFVFFFFCKTVLG

Target ORF information:

RefSeq Version	NM_001107549.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus trinucleotide repeat containing adaptor 6A (Tnrc6a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107549.1

ORF Insert Sequence:

ATGGAAGAAA AGAAAAGGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CTAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CCGATAACGG CACTTCCACA GCTACCAGCA CTAATAATAA TGCCAAGCGA 
GCTACAGCCA GCAATCAGCA GCCGCCGCCG CCGCCGCAGC AGCCGCAGCA GCAGCAGCAG 
CAGCAGCAGC AACAACAAGA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCCACAAG CCTTGCCTCG GTATCCTCGT GAAGTACCTC CACGATTTCG CCACCAGGAA 
CACAAACAGC TTCTAAAGAG GGGTCAGCAT TTTCCAGTCA TAGCAGCAAA CTTGGGATCT 
GCTGTTAAGG TGTTAAACAG CCAATCAGAA AGCAGTGCTG TAACAAATCA ACAGCCACAA 
AATAACGGAG AGGTGCAGAA CAGCAAAAGC CAGTCAGATA TAAATCATAA TACTTCAGGA 
TCCCATTATG AAAATTGCCA GCGGGGACCT GTGTCTTCTA CAAGTGACTG TAGCACAAGC 
TGTAAGAATG CTGTAAACGA CTTGTTGGAA AAAGAAGCAT GGCCCTCAGC CCCTGGCAGT 
GATCCTGAGT TGGCTTCAGA ATGTATAGAT GCTGATTCTG CCTCCAATTC TGAGTCAGAG 
AGAAACATCA CTGTCATGGC TTCAGGGAAC ACAGGTGGTG AGAAAGATGG CCTTCGGAAC 
AGCACTGGAC TCGGTCCCCA AAGCAAATTT GTGGTTGGTA GCAGCAGCAA TAATGTGGGT 
CATGGAAGTA GTACTGGGCC ATGGGGCTTT CCCCATGGAG CCATAATAAG CACATGTCAG 
GTCTCTGTGG ATGCTCCTGA AAGCAAACCA GAAAGTAGTA ACAGTAGAAT GAATGCTTGG 
GGCACTGTAA GTTCTTCATC AAATGGAGGG TTAAATCCAA GCACTTTGAA TTCAGCTAGC 
AACCATGGTG CCTGGCCAGT ATTAGAAAAC AACGGACTTG CCCTAAAAGG GCCTGTAGGG 
AGTGGGAATT CTGGCATCAA TATTCAGTGT AGTACCATAG GCCAGATGCC TAACAATCAG 
AGTATCAACT CTAAAGTCAG TGGCTCTTCT ACCCATGGTA CCTGGGGTAG CCTTCAGGAA 
ACTTGTGAGC CTGAAGTAAG TGGTTCACAG AAGGTTTCAT TCAGTGGTCA ACCTCAGAAT 
ATCACCACTG AAACGACTGG ACCAAATAAC ACTACTAACT TTATGACCTC TAGTTTACCA 
AACTCCGGTT CAGTACAAAA TAATGAACTG CCTACTAGTA ATCCAGGGGC CTGGCGTGTG 
AGCACAATGA ATCATCCTCA GATACAGGCT CCGTCAGTTC TGAATGGCAC TTCCCTTTCT 
CACCTTAGTA ATGGAGAGTC AAAAACTGGA GGCTCCTACG GTACTACATG GGGTGCCTAT 
GGTTCTAATT ACTCTGGCGA CAAATGTGCA GGCCCTAATG GCCAAGCCAA TGGTGACACT 
GTGAATGCAA CTCTAATGCA GCCTGGCATA AATGGGCCTA TGGGCACTAA CTTTCAAGTT 
AGTACAAATA AAGGGGGAGG TGTATGGGAG CCTGGGACAG TGAATTCCCA GAGTTCACCA 
TGGGGAAGTG GAAATGGTGC AAATTCTGGA GGAAGTCGAA GAGGATGGGG AAGTCCTGCA 
CAGAACACTG GCACTAGTCT GTCCAGTGTT GAGTGGAACA AACTGCCTAG CAACCAGCAT 
TCCAATGACA GTGCAAATGG CAATGGTAGT AAGAAACTTA CAAATGGATG GAAATCTACT 
GAGGAAGATG ATCAGGGTTC TGGCACATCT CAGACGAATG AGCAAAACAG TGTGTGGGCC 
AAAGCAGGAG GCACAGTGGA GAGTGATGGT AGTGCAGAGA GCACTGGACG CCTTGAAGAA 
AAAGTAACCG GGGAAAGCCA GAGTAGAGAT AGAAGAAAAA TTGATCAGCA CACATTACTC 
CAAAGCATTG TAAACAGAAC TGACTTAGAT CCACGTGTCC TATCCAATTC TGGGTGGGGA 
CAGACTCCTA TTAAGCAGAA TACTGCCTGG GATACAGAGA CATCACCAAG AGGGGAAAGA 
AAGACTGACA ATGGGACAGA GGCCTGGGGA AGCTCTGCAA CACAGACTTT TAACTCAGGG 
GCATGTACAG ATAAGACTAG CCCTAATAGT AATGATACCT CATCTGTATC AGGGTGGGGT 
GATCCCAAAC CTACTCTGAG GTGGGGAGAT TCCAAAGGCT CAAACTGCCA GGGGGGGTGG 
GAAGATGATT CTGCTGCTAC AGGAATGATC AAGAGCAATC AGTGGGGGCA TTGCAAAGAA 
GACAAGTCTG CATGGAATGA TTCGCAAAAG AACAAACAAG GCTGGGGCGA TGGACAAAAG 
TCAAGCCAAG GTTGGTCCGT TTCTGCCAGT GATAACTGGG GAGAATCTTC CAGGAGTAAC 
CATTGGGGTG AGGCTAATAA GAAATCCAGC TCAGGAGGCA GTGACAGCGA CAGGTCCATT 
TCTGGTTGGA ACGAACTTGG TAAAACTAGT TCTTTTACTT GGGGAAATAA TATAAATCCA 
AATAACTCAT CAGGATGGGA TGAATCTTCT AAACCTAACT CTTCCCAGGG ATGGGGAGAC 
CCTCCAAAGT GTAATCAGTC CCTAGGTTGG GGAGATTCAT CAAAACCAGT TAGTTCTCCA 
GATTGGAACA AGCAACAAGA CATTGTTGGA TCGTGGGGAA TCCCACCAGC CACCAGCAAG 
CCTCCTGGTA CAGGCTGGCT TGGGGGACCT ATTCCTGCTC CAGCAAAGGA GGAAGAACCC 
ACAGGCTGGG AGGAACCATC CCCAGAGTCT ATACGACGAA AAATGGAGAT CGATGATGGA 
ACTTCAGCTT GGGGAGATCC AAGCAAATAC AACTACAAAA ATGTGAACAT GTGGAATAAA 
AACATCCCAG AAGGCAGCAG CCGCTCAGAC CAGCAAGCAC AGATGCACCG GTTGTTGCCG 
GCTGCAAGTG CCATCTCAAG CAAGGAGACC AGCAGTGGCT CTGGCTGGGG TGAGCCTTGG 
GCAGAGCCTT CTACTCCAGC CACGACTGTG GATAATGGTA CTTCAGCCTG GGGCAAGCCC 
ATAGACAGTG GTCCCAGCTG GGGAGAACCC ATTACTGCAG CATCCAGTGC ATCCACGTGG 
GGCTCCAACT CTGTTGGTCC ACAATCATTA AGCAAATCTG GACCCAAATC TATGCAAGAT 
GGCTGGTGTG GTGAGGACAC GCCATTGCCT GGAAGTCGCC CCACTGGCTG GGAAGAGGAG 
GAGGATGTAG AGATTGGGAT GTGGAACAGT AGCTCATCTC AAGAGCTTAA CTCATCCTTA 
AACTGGCCAC CATATACCAA GAAAATGTCA TCCAAGGGTC TGAGTGGCAA AAAAAGGAGA 
AGGGAAAGGG GAATGATGAA AGGCGGAAAC AAACAAGAGG ACGCGTGGAT AAACCCATTC 
GTTAAGCAGT TTTCAAATAT CAGTTTTTCA AGAGACTCAC CAGAAGAAAA TGTACAGAGC 
AATAAGATGG ATCTTTCTGG AGGAATGTTA CAGGACAAGC GGATGGAGAT TGATAAGCAT 
AGCCTGAATA TTGGTGACTA CAATCGAGCG GTCGGGAAAG GCCCTGGGTC TCGGCCTCAG 
ATTTCCAAAG AGTCTTCCAT GGAGCGCAAT CCTTACTTTG ATAAGGATGG CATTGTAGCA 
GATGAATCCC AAAACATGCA GTTTATGTCC AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT 
AATGCACTAC CTAACCAGGC CCTTGGCTCC ATAGCAGGGC TGGGTACGCA AAACTTGAAT 
TCTGTTAGAC AGAATGGCAA TCCCAGTATG TTTGGTGTTG GAAACACAGC AGCACAACCC 
CGGGGCATGC AGCAGCCTCC AGCACAACCT CTCAGTTCAT CTCAGCCTAA TCTTCGTGCT 
CAAGTGCCTC CTCCATTACT CTCCCCTCAG GTTCCAGTTT CATTGCTGAA GTATGCACCA 
AACAACGGTG GCCTGAATCC GCTCTTTGGC CCTCAACAGG TAGCCATGCT GAACCAGCTA 
TCCCAACTAA ACCAGCTTTC TCAGATCTCC CAGTTACAGC GATTGTTAGC GCAGCAGCAG 
AGGGCACAGA GTCAGAGAAG CGTGCCTTCT GCTAACCGAC AGCAGCAAGA CCAGCAGGGT 
CGACCTCTTA GTGTACAGCA GCAGATGATG CAACAGTCTC GTCAGCTTGA TCCAAGCCTG 
CTGGTGAAGC AGCAGACTCC GCCTTCTCAG CAGCCGCTCC ATCAGCCAGC CATGAAGTCC 
TTCCTTGACA ATGTCATGCC CCACACTACA CCCGAGCTAC AGAAAGGGCC ATCGCCAGTA 
AACGCTTTCA GCAGCTTCCC TATAGGCTTG AACTCAAACT TGAATGTAAA TATGGATATG 
AACAGTATTA AAGAGCCACA GTCACGACTA AGGAAGTGGA CAACAGTGGA CAGCATGTCT 
GTGAACACGT CTTTGGATCA AAACTCCAGC AAACATGGTG CTATTTCAAG TGGTTTTAGG 
CTGGAAGAGT CTCCATTTGT TCCCTATGAC TTTATGAATA GCAGTACTTC ACCAGCCAGT 
CCTCCAGGTT CAATAGGAGA TGGCTGGCCA CGTGCCAAAT CGCCTAACGG CTCTAGCAGT 
GTTAACTGGC CACCAGAATT TCGCCCTGGT GAACCATGGA AAGGTTATCC AAACATTGAC 
CCTGAAACTG ACCCTTACGT CACTCCTGGC AGTGTCATAA ACAGTCTTTC AATTAATACT 
GTGCGGGAAG TTGACCACCT CAGGGACAGG AACAGTGGGT CATCCTCATC CTTGAACACC 
ACGCTGCCTT CAACTAGTGC CTGGTCATCC ATTCGTGCCT CCAACTACAA TGTTCCCCTC 
AGCAGTACAG CACAAAGCAC TTCAGCCAGA AATAGTGATT CCAAATTGAC GTGGTCTCCT 
GGTTCAGTTA CAAACACCTC TCTGGCTCAT GAGCTGTGGA AAGTCCCTTT ACCACCTAAA 
AACATCACTG CTCCGTCCCG CCCACCTCCG GGGCTGACTG GTCAGAAGCC ACCCTTGTCT 
ACGTGGGATA ATTCTCCCCT TCGTGTAGGT GGAGGATGGG GAAATTCTGA CGCCAGATAT 
ACCCCAGGCT CCAGCTGGGG TGAGAGCAGC TCAGGGAGAA TAACCAATTG GCTTGTTCTG 
AAGAACCTCA CACCTCAGAT TGACGGCTCC ACTCTGCGCA CCCTGTGCAT GCAGCATGGC 
CCACTGATTA CATTCCATCT GAACCTCCCA CATGGAAATG CTCTGGTCCG TTACAGTTCA 
AAGGAAGAGG TAGTGAAGGC ACAAAAGTCT CTGCACATGT GTGTGCTGGG GAACACTACT 
ATTCTCGCTG AGTTTGCCAG TGAAGAGGAG ATCAGTCGCT TCTTTGCACA AAGTCAGTCT 
CTGACCCCTT CTCCTGGCTG GCAGTCTCTC GGGTCCAGCC AGAGCCGGCT GGGCTCCCTC 
GACTGTTCCC ACTCATTCTC CAGCCGGACT GATCTCAATC ACTGGAATGG TGCTGGGCTG 
TCGGGAACTA ACTGTGGAGA CCTTCACGGC ACTTCCCTCT GGGGGACCCC ACATTATTCC 
ACAAGCCTGT GGGGTCCCCC AAGCAGCAGT GACCCCCGGG GAATTAGCAG CCCATCCCCC 
ATTAATGCTT TTCTTTCTGT TGACCACCTG GCTTTTATTT TTATTTTTTT TTTGTTTTTT 
TGTTTTTTTG TTTTTTTTTT TTTTTGCAAA ACCGTTTTGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnrc6a ( XM_017589209.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444698.1 Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X5, mRNA.

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CloneID	ORa23390
Clone ID Related Accession (Same CDS sequence)	XM_017589209.1
Accession Version	XM_017589209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGTCTG AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA 
ATGATGAAAG GCGGAAACAA ACAAGAGGAC GCGTGGATAA ACCCATTCGT TAAGCAGTTT 
TCAAATATCA GTTTTTCAAG AGACTCACCA GAAGAAAATG TACAGAGCAA TAAGATGGAT 
CTTTCTGGAG GAATGTTACA GGACAAGCGG ATGGAGATTG ATAAGCATAG CCTGAATATT 
GGTGACTACA ATCGAGCGGT CGGGAAAGGC CCTGGGTCTC GGCCTCAGAT TTCCAAAGAG 
TCTTCCATGG AGCGCAATCC TTACTTTGAT AAGAATGGCA ATCCCAGTAT GTTTGGTGTT 
GGAAACACAG CAGCACAACC CCGGGGCATG CAGCAGCCTC CAGCACAACC TCTCAGTTCA 
TCTCAGCCTA ATCTTCGTGC TCAAGTGCCT CCTCCATTAC TCTCCCCTCA GGTTCCAGTT 
TCATTGCTGA AGTATGCACC AAACAACGGT GGCCTGAATC CGCTCTTTGG CCCTCAACAG 
GTAGCCATGC TGAACCAGCT ATCCCAACTA AACCAGCTTT CTCAGATCTC CCAGTTACAG 
CGATTGTTAG CGCAGCAGCA GAGGGCACAG AGTCAGAGAA GCGTGCCTTC TGCTAACCGA 
CAGCAGCAAG ACCAGCAGGG TCGACCTCTT AGTGTACAGC AGCAGATGAT GCAACAGTCT 
CGTCAGCTTG ATCCAAGCCT GCTGGTGAAG CAGCAGACTC CGCCTTCTCA GCAGCCGCTC 
CATCAGCCAG CCATGAAGTC CTTCCTTGAC AATGTCATGC CCCACACTAC ACCCGAGCTA 
CAGAAAGGGC CATCGCCAGT AAACGCTTTC AGCAGCTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCACGACT AAGGAAGTGG 
ACAACAGTGG ACAGCATGTC TGTGAACACG TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTTAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAT 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAGTCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ATGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CGTGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAAGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGGCTGACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AATTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGATA TACCCCAGGC TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACCAATT GGCTTGTTCT GAAGAACCTC ACACCTCAGA TTGACGGCTC CACTCTGCGC 
ACCCTGTGCA TGCAGCATGG CCCACTGATT ACATTCCATC TGAACCTCCC ACATGGAAAT 
GCTCTGGTCC GTTACAGTTC AAAGGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTGCTGG GGAACACTAC TATTCTCGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGC 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCTGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC TGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCCC CAAGCAGCAG TGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444698.1
CDS	301..6075
Translation	MQLVAEPGLEARCPGSQDTPLPEEWNRDLVQEEEQLMEEKKRKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSDNGTSTATSTNNNAKRATASNQQPPPPQQQPQQQQQQQQQQQEQQQQQQQQQQQQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSAVTNQQPQNNGEVQNSKSQSDINHNTSGSHYENCQRGPVSSTSDCSTSCKNAVNDLLEKEAWPSAPGSDPELASECIDADSASNSESERNITVMASGNTGGEKDGLRNSTGLGPQSKFVVGSSSNNVGHGSSTGPWGFPHGAIISTCQVSVDAPESKPESSNSRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGNSGINIQCSTIGQMPNNQSINSKVSGSSTHGTWGSLQETCEPEVSGSQKVSFSGQPQNITTETTGPNNTTNFMTSSLPNSGSVQNNELPTSNPGAWRVSTMNHPQIQAPSVLNGTSLSHLSNGESKTGGSYGTTWGAYGSNYSGDKCAGPNGQANGDTVNATLMQPGINGPMGTNFQVSTNKGGGVWEPGTVNSQSSPWGSGNGANSGGSRRGWGSPAQNTGTSLSSVEWNKLPSNQHSNDSANGNGSKKLTNGWKSTEEDDQGSGTSQTNEQNSVWAKAGGTVESDGSAESTGRLEEKVTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPNSNDTSSVSGWGDPKPTLRWGDSKGSNCQGGWEDDSAATGMIKSNQWGHCKEDKSAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGESSRSNHWGEANKKSSSGGSDSDRSISGWNELGKTSSFTWGNNINPNNSSGWDESSKPNSSQGWGDPPKCNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATSKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNIPEGSSRSDQQAQMHRLLPAASAISSKETSSGSGWGEPWAEPSTPATTVDNGTSAWGKPIDSGPSWGEPITAASSASTWGSNSVGPQSLSKSGPKSMQDGWCGEDTPLPGSRPTGWEEEEDVEIGMWNSSSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEDAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRAVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSANRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQPLHQPAMKSFLDNVMPHTTPELQKGPSPVNAFSSFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSMSVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINSLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017589209.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589209.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGTCTG AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA 
ATGATGAAAG GCGGAAACAA ACAAGAGGAC GCGTGGATAA ACCCATTCGT TAAGCAGTTT 
TCAAATATCA GTTTTTCAAG AGACTCACCA GAAGAAAATG TACAGAGCAA TAAGATGGAT 
CTTTCTGGAG GAATGTTACA GGACAAGCGG ATGGAGATTG ATAAGCATAG CCTGAATATT 
GGTGACTACA ATCGAGCGGT CGGGAAAGGC CCTGGGTCTC GGCCTCAGAT TTCCAAAGAG 
TCTTCCATGG AGCGCAATCC TTACTTTGAT AAGAATGGCA ATCCCAGTAT GTTTGGTGTT 
GGAAACACAG CAGCACAACC CCGGGGCATG CAGCAGCCTC CAGCACAACC TCTCAGTTCA 
TCTCAGCCTA ATCTTCGTGC TCAAGTGCCT CCTCCATTAC TCTCCCCTCA GGTTCCAGTT 
TCATTGCTGA AGTATGCACC AAACAACGGT GGCCTGAATC CGCTCTTTGG CCCTCAACAG 
GTAGCCATGC TGAACCAGCT ATCCCAACTA AACCAGCTTT CTCAGATCTC CCAGTTACAG 
CGATTGTTAG CGCAGCAGCA GAGGGCACAG AGTCAGAGAA GCGTGCCTTC TGCTAACCGA 
CAGCAGCAAG ACCAGCAGGG TCGACCTCTT AGTGTACAGC AGCAGATGAT GCAACAGTCT 
CGTCAGCTTG ATCCAAGCCT GCTGGTGAAG CAGCAGACTC CGCCTTCTCA GCAGCCGCTC 
CATCAGCCAG CCATGAAGTC CTTCCTTGAC AATGTCATGC CCCACACTAC ACCCGAGCTA 
CAGAAAGGGC CATCGCCAGT AAACGCTTTC AGCAGCTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCACGACT AAGGAAGTGG 
ACAACAGTGG ACAGCATGTC TGTGAACACG TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTTAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAT 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAGTCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ATGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CGTGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAAGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGGCTGACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AATTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGATA TACCCCAGGC TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACCAATT GGCTTGTTCT GAAGAACCTC ACACCTCAGA TTGACGGCTC CACTCTGCGC 
ACCCTGTGCA TGCAGCATGG CCCACTGATT ACATTCCATC TGAACCTCCC ACATGGAAAT 
GCTCTGGTCC GTTACAGTTC AAAGGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTGCTGG GGAACACTAC TATTCTCGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGC 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCTGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC TGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCCC CAAGCAGCAG TGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnrc6a ( XM_008759747.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757969.1 Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X1, mRNA.

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CloneID	ORa46682
Clone ID Related Accession (Same CDS sequence)	XM_008759747.2
Accession Version	XM_008759747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGTCTG AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA 
ATGATGAAAG GCGGAAACAA ACAAGAGGAC GCGTGGATAA ACCCATTCGT TAAGCAGTTT 
TCAAATATCA GTTTTTCAAG AGACTCACCA GAAGAAAATG TACAGAGCAA TAAGATGGAT 
CTTTCTGGAG GAATGTTACA GGACAAGCGG ATGGAGATTG ATAAGCATAG CCTGAATATT 
GGTGACTACA ATCGAGCGGT CGGGAAAGGC CCTGGGTCTC GGCCTCAGAT TTCCAAAGAG 
TCTTCCATGG AGCGCAATCC TTACTTTGAT AAGGATGGCA TTGTAGCAGA TGAATCCCAA 
AACATGCAGT TTATGTCCAG TCAAAGCATG AAGCTTCCCC CTTCAAATAA TGCACTACCT 
AACCAGGCCC TTGGCTCCAT AGCAGGGCTG GGTACGCAAA ACTTGAATTC TGTTAGACAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT CAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT TCCAGTTTCA TTGCTGAAGT ATGCACCAAA CAACGGTGGC 
CTGAATCCGC TCTTTGGCCC TCAACAGGTA GCCATGCTGA ACCAGCTATC CCAACTAAAC 
CAGCTTTCTC AGATCTCCCA GTTACAGCGA TTGTTAGCGC AGCAGCAGAG GGCACAGAGT 
CAGAGAAGCG TGCCTTCTGC TAACCGACAG CAGCAAGACC AGCAGGGTCG ACCTCTTAGT 
GTACAGCAGC AGATGATGCA ACAGTCTCGT CAGCTTGATC CAAGCCTGCT GGTGAAGCAG 
CAGACTCCGC CTTCTCAGCA GCCGCTCCAT CAGCCAGCCA TGAAGTCCTT CCTTGACAAT 
GTCATGCCCC ACACTACACC CGAGCTACAG AAAGGGCCAT CGCCAGTAAA CGCTTTCAGC 
AGCTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CACGACTAAG GAAGTGGACA ACAGTGGACA GCATGTCTGT GAACACGTCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTTAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAATAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAACTGGCCA 
CCAGAATTTC GCCCTGGTGA ACCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AGTCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAATG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACGT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAA GTCCCTTTAC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG GCTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTGTAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGCTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACCAATTGGC TTGTTCTGAA GAACCTCACA 
CCTCAGATTG ACGGCTCCAC TCTGCGCACC CTGTGCATGC AGCATGGCCC ACTGATTACA 
TTCCATCTGA ACCTCCCACA TGGAAATGCT CTGGTCCGTT ACAGTTCAAA GGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTGCTGGGGA ACACTACTAT TCTCGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGCTTC TTTGCACAAA GTCAGTCTCT GACCCCTTCT 
CCTGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACTGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCCCTCTGG GGGACCCCAC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGTGA CCCCCGGGGA ATTAGCAGCC CATCCCCCAT TAATGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757969.1
CDS	302..6223
Translation	MQLVAEPGLEARCPGSQDTPLPEEWNRDLVQEEEQLMEEKKRKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSDNGTSTATSTNNNAKRATASNQQPPPPQQQPQQQQQQQQQQQEQQQQQQQQQQQQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSAVTNQQPQNNGEVQNSKSQSDINHNTSGSHYENCQRGPVSSTSDCSTSCKNAVNDLLEKEAWPSAPGSDPELASECIDADSASNSESERNITVMASGNTGGEKDGLRNSTGLGPQSKFVVGSSSNNVGHGSSTGPWGFPHGAIISTCQVSVDAPESKPESSNSRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGNSGINIQCSTIGQMPNNQSINSKVSGSSTHGTWGSLQETCEPEVSGSQKVSFSGQPQNITTETTGPNNTTNFMTSSLPNSGSVQNNELPTSNPGAWRVSTMNHPQIQAPSVLNGTSLSHLSNGESKTGGSYGTTWGAYGSNYSGDKCAGPNGQANGDTVNATLMQPGINGPMGTNFQVSTNKGGGVWEPGTVNSQSSPWGSGNGANSGGSRRGWGSPAQNTGTSLSSVEWNKLPSNQHSNDSANGNGSKKLTNGWKSTEEDDQGSGTSQTNEQNSVWAKAGGTVESDGSAESTGRLEEKVTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPNSNDTSSVSGWGDPKPTLRWGDSKGSNCQGGWEDDSAATGMIKSNQWGHCKEDKSAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGESSRSNHWGEANKKSSSGGSDSDRSISGWNELGKTSSFTWGNNINPNNSSGWDESSKPNSSQGWGDPPKCNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATSKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNIPEGSSRSDQQAQMHRLLPAASAISSKETSSGSGWGEPWAEPSTPATTVDNGTSAWGKPIDSGPSWGEPITAASSASTWGSNSVGPQSLSKSGPKSMQDGWCGEDTPLPGSRPTGWEEEEDVEIGMWNSSSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEDAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRAVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNNALPNQALGSIAGLGTQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSANRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQPLHQPAMKSFLDNVMPHTTPELQKGPSPVNAFSSFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSMSVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINSLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_008759747.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759747.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGTCTG AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA 
ATGATGAAAG GCGGAAACAA ACAAGAGGAC GCGTGGATAA ACCCATTCGT TAAGCAGTTT 
TCAAATATCA GTTTTTCAAG AGACTCACCA GAAGAAAATG TACAGAGCAA TAAGATGGAT 
CTTTCTGGAG GAATGTTACA GGACAAGCGG ATGGAGATTG ATAAGCATAG CCTGAATATT 
GGTGACTACA ATCGAGCGGT CGGGAAAGGC CCTGGGTCTC GGCCTCAGAT TTCCAAAGAG 
TCTTCCATGG AGCGCAATCC TTACTTTGAT AAGGATGGCA TTGTAGCAGA TGAATCCCAA 
AACATGCAGT TTATGTCCAG TCAAAGCATG AAGCTTCCCC CTTCAAATAA TGCACTACCT 
AACCAGGCCC TTGGCTCCAT AGCAGGGCTG GGTACGCAAA ACTTGAATTC TGTTAGACAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT CAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT TCCAGTTTCA TTGCTGAAGT ATGCACCAAA CAACGGTGGC 
CTGAATCCGC TCTTTGGCCC TCAACAGGTA GCCATGCTGA ACCAGCTATC CCAACTAAAC 
CAGCTTTCTC AGATCTCCCA GTTACAGCGA TTGTTAGCGC AGCAGCAGAG GGCACAGAGT 
CAGAGAAGCG TGCCTTCTGC TAACCGACAG CAGCAAGACC AGCAGGGTCG ACCTCTTAGT 
GTACAGCAGC AGATGATGCA ACAGTCTCGT CAGCTTGATC CAAGCCTGCT GGTGAAGCAG 
CAGACTCCGC CTTCTCAGCA GCCGCTCCAT CAGCCAGCCA TGAAGTCCTT CCTTGACAAT 
GTCATGCCCC ACACTACACC CGAGCTACAG AAAGGGCCAT CGCCAGTAAA CGCTTTCAGC 
AGCTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CACGACTAAG GAAGTGGACA ACAGTGGACA GCATGTCTGT GAACACGTCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTTAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAATAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAACTGGCCA 
CCAGAATTTC GCCCTGGTGA ACCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AGTCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAATG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACGT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAA GTCCCTTTAC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG GCTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTGTAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGCTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACCAATTGGC TTGTTCTGAA GAACCTCACA 
CCTCAGATTG ACGGCTCCAC TCTGCGCACC CTGTGCATGC AGCATGGCCC ACTGATTACA 
TTCCATCTGA ACCTCCCACA TGGAAATGCT CTGGTCCGTT ACAGTTCAAA GGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTGCTGGGGA ACACTACTAT TCTCGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGCTTC TTTGCACAAA GTCAGTCTCT GACCCCTTCT 
CCTGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACTGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCCCTCTGG GGGACCCCAC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGTGA CCCCCGGGGA ATTAGCAGCC CATCCCCCAT TAATGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnrc6a ( XM_008759748.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757970.1 Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X2, mRNA.

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CloneID	ORa46683
Clone ID Related Accession (Same CDS sequence)	XM_008759748.2
Accession Version	XM_008759748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGT TGGAAGCTAA AGCTACCAAA GACGTAGAGA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAGGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCTAGTGTAA GCCAGCCTCA GCCTGCCAAC TCCGATAACG GCACTTCCAC AGCTACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AGCAATCAGC AGCCGCCGCC GCCGCAGCAG 
CAGCCGCAGC AGCAGCAGCA GCAGCAGCAG CAACAACAAG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCCACAA GCCTTGCCTC GGTATCCTCG TGAAGTACCT 
CCACGATTTC GCCACCAGGA ACACAAACAG CTTCTAAAGA GGGGTCAGCA TTTTCCAGTC 
ATAGCAGCAA ACTTGGGATC TGCTGTTAAG GTGTTAAACA GCCAATCAGA AAGCAGTGCT 
GTAACAAATC AACAGCCACA AAATAACGGA GAGGTGCAGA ACAGCAAAAG CCAGTCAGAT 
ATAAATCATA ATACTTCAGG ATCCCATTAT GAAAATTGCC AGCGGGGACC TGTGTCTTCT 
ACAAGTGACT GTAGCACAAG CTGTAAGAAT GCTGTAAACG ACTTGTTGGA AAAAGAAGCA 
TGGCCCTCAG CCCCTGGCAG TGATCCTGAG TTGGCTTCAG AATGTATAGA TGCTGATTCT 
GCCTCCAATT CTGAGTCAGA GAGAAACATC ACTGTCATGG CTTCAGGGAA CACAGGTGGT 
GAGAAAGATG GCCTTCGGAA CAGCACTGGA CTCGGTCCCC AAAGCAAATT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG TCATGGAAGT AGTACTGGGC CATGGGGCTT TCCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAACC AGAAAGTAGT 
AACAGTAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGAAAA CAACGGACTT 
GCCCTAAAAG GGCCTGTAGG GAGTGGGAAT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATCAAC TCTAAAGTCA GTGGCTCTTC TACCCATGGT 
ACCTGGGGTA GCCTTCAGGA AACTTGTGAG CCTGAAGTAA GTGGTTCACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATCACCACT GAAACGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAAA ATAATGAACT GCCTACTAGT 
AATCCAGGGG CCTGGCGTGT GAGCACAATG AATCATCCTC AGATACAGGC TCCGTCAGTT 
CTGAATGGCA CTTCCCTTTC TCACCTTAGT AATGGAGAGT CAAAAACTGG AGGCTCCTAC 
GGTACTACAT GGGGTGCCTA TGGTTCTAAT TACTCTGGCG ACAAATGTGC AGGCCCTAAT 
GGCCAAGCCA ATGGTGACAC TGTGAATGCA ACTCTAATGC AGCCTGGCAT AAATGGGCCT 
ATGGGCACTA ACTTTCAAGT TAGTACAAAT AAAGGGGGAG GTGTATGGGA GCCTGGGACA 
GTGAATTCCC AGAGTTCACC ATGGGGAAGT GGAAATGGTG CAAATTCTGG AGGAAGTCGA 
AGAGGATGGG GAAGTCCTGC ACAGAACACT GGCACTAGTC TGTCCAGTGT TGAGTGGAAC 
AAACTGCCTA GCAACCAGCA TTCCAATGAC AGTGCAAATG GCAATGGTAG TAAGAAACTT 
ACAAATGGAT GGAAATCTAC TGAGGAAGAT GATCAGGGTT CTGGCACATC TCAGACGAAT 
GAGCAAAACA GTGTGTGGGC CAAAGCAGGA GGCACAGTGG AGAGTGATGG TAGTGCAGAG 
AGCACTGGAC GCCTTGAAGA AAAAGTAACC GGGGAAAGCC AGAGTAGAGA TAGAAGAAAA 
ATTGATCAGC ACACATTACT CCAAAGCATT GTAAACAGAA CTGACTTAGA TCCACGTGTC 
CTATCCAATT CTGGGTGGGG ACAGACTCCT ATTAAGCAGA ATACTGCCTG GGATACAGAG 
ACATCACCAA GAGGGGAAAG AAAGACTGAC AATGGGACAG AGGCCTGGGG AAGCTCTGCA 
ACACAGACTT TTAACTCAGG GGCATGTACA GATAAGACTA GCCCTAATAG TAATGATACC 
TCATCTGTAT CAGGGTGGGG TGATCCCAAA CCTACTCTGA GGTGGGGAGA TTCCAAAGGC 
TCAAACTGCC AGGGGGGGTG GGAAGATGAT TCTGCTGCTA CAGGAATGAT CAAGAGCAAT 
CAGTGGGGGC ATTGCAAAGA AGACAAGTCT GCATGGAATG ATTCGCAAAA GAACAAACAA 
GGCTGGGGCG ATGGACAAAA GTCAAGCCAA GGTTGGTCCG TTTCTGCCAG TGATAACTGG 
GGAGAATCTT CCAGGAGTAA CCATTGGGGT GAGGCTAATA AGAAATCCAG CTCAGGAGGC 
AGTGACAGCG ACAGGTCCAT TTCTGGTTGG AACGAACTTG GTAAAACTAG TTCTTTTACT 
TGGGGAAATA ATATAAATCC AAATAACTCA TCAGGATGGG ATGAATCTTC TAAACCTAAC 
TCTTCCCAGG GATGGGGAGA CCCTCCAAAG TGTAATCAGT CCCTAGGTTG GGGAGATTCA 
TCAAAACCAG TTAGTTCTCC AGATTGGAAC AAGCAACAAG ACATTGTTGG ATCGTGGGGA 
ATCCCACCAG CCACCAGCAA GCCTCCTGGT ACAGGCTGGC TTGGGGGACC TATTCCTGCT 
CCAGCAAAGG AGGAAGAACC CACAGGCTGG GAGGAACCAT CCCCAGAGTC TATACGACGA 
AAAATGGAGA TCGATGATGG AACTTCAGCT TGGGGAGATC CAAGCAAATA CAACTACAAA 
AATGTGAACA TGTGGAATAA AAACATCCCA GAAGGCAGCA GCCGCTCAGA CCAGCAAGCA 
CAGATGCACC GGTTGTTGCC GGCTGCAAGT GCCATCTCAA GCAAGGAGAC CAGCAGTGGC 
TCTGGCTGGG GTGAGCCTTG GGCAGAGCCT TCTACTCCAG CCACGACTGT GGATAATGGT 
ACTTCAGCCT GGGGCAAGCC CATAGACAGT GGTCCCAGCT GGGGAGAACC CATTACTGCA 
GCATCCAGTG CATCCACGTG GGGCTCCAAC TCTGTTGGTC CACAATCATT AAGCAAATCT 
GGACCCAAAT CTATGCAAGA TGGCTGGTGT GGTGAGGACA CGCCATTGCC TGGAAGTCGC 
CCCACTGGCT GGGAAGAGGA GGAGGATGTA GAGATTGGGA TGTGGAACAG TAGCTCATCT 
CAAGAGCTTA ACTCATCCTT AAACTGGCCA CCATATACCA AGAAAATGTC ATCCAAGGGT 
CTGAGTGGCA AAAAAAGGAG AAGGGAAAGG GGAATGATGA AAGGCGGAAA CAAACAAGAG 
GACGCGTGGA TAAACCCATT CGTTAAGCAG TTTTCAAATA TCAGTTTTTC AAGAGACTCA 
CCAGAAGAAA ATGTACAGAG CAATAAGATG GATCTTTCTG GAGGAATGTT ACAGGACAAG 
CGGATGGAGA TTGATAAGCA TAGCCTGAAT ATTGGTGACT ACAATCGAGC GGTCGGGAAA 
GGCCCTGGGT CTCGGCCTCA GATTTCCAAA GAGTCTTCCA TGGAGCGCAA TCCTTACTTT 
GATAAGGATG GCATTGTAGC AGATGAATCC CAAAACATGC AGTTTATGTC CAGTCAAAGC 
ATGAAGCTTC CCCCTTCAAA TAATGCACTA CCTAACCAGG CCCTTGGCTC CATAGCAGGG 
CTGGGTACGC AAAACTTGAA TTCTGTTAGA CAGAATGGCA ATCCCAGTAT GTTTGGTGTT 
GGAAACACAG CAGCACAACC CCGGGGCATG CAGCAGCCTC CAGCACAACC TCTCAGTTCA 
TCTCAGCCTA ATCTTCGTGC TCAAGTGCCT CCTCCATTAC TCTCCCCTCA GGTTCCAGTT 
TCATTGCTGA AGTATGCACC AAACAACGGT GGCCTGAATC CGCTCTTTGG CCCTCAACAG 
GTAGCCATGC TGAACCAGCT ATCCCAACTA AACCAGCTTT CTCAGATCTC CCAGTTACAG 
CGATTGTTAG CGCAGCAGCA GAGGGCACAG AGTCAGAGAA GCGTGCCTTC TGCTAACCGA 
CAGCAGCAAG ACCAGCAGGG TCGACCTCTT AGTGTACAGC AGCAGATGAT GCAACAGTCT 
CGTCAGCTTG ATCCAAGCCT GCTGGTGAAG CAGCAGACTC CGCCTTCTCA GCAGCCGCTC 
CATCAGCCAG CCATGAAGTC CTTCCTTGAC AATGTCATGC CCCACACTAC ACCCGAGCTA 
CAGAAAGGGC CATCGCCAGT AAACGCTTTC AGCAGCTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCACGACT AAGGAAGTGG 
ACAACAGTGG ACAGCATGTC TGTGAACACG TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTTAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAT 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAGTCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ATGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CGTGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAAGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGGCTGACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AATTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGATA TACCCCAGGC TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACCAATT GGCTTGTTCT GAAGAACCTC ACACCTCAGA TTGACGGCTC CACTCTGCGC 
ACCCTGTGCA TGCAGCATGG CCCACTGATT ACATTCCATC TGAACCTCCC ACATGGAAAT 
GCTCTGGTCC GTTACAGTTC AAAGGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTGCTGG GGAACACTAC TATTCTCGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGC 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCTGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC TGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCCC CAAGCAGCAG TGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757970.1
CDS	459..6353
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKRKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSDNGTSTATSTNNNAKRATASNQQPPPPQQQPQQQQQQQQQQQEQQQQQQQQQQQQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSAVTNQQPQNNGEVQNSKSQSDINHNTSGSHYENCQRGPVSSTSDCSTSCKNAVNDLLEKEAWPSAPGSDPELASECIDADSASNSESERNITVMASGNTGGEKDGLRNSTGLGPQSKFVVGSSSNNVGHGSSTGPWGFPHGAIISTCQVSVDAPESKPESSNSRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGNSGINIQCSTIGQMPNNQSINSKVSGSSTHGTWGSLQETCEPEVSGSQKVSFSGQPQNITTETTGPNNTTNFMTSSLPNSGSVQNNELPTSNPGAWRVSTMNHPQIQAPSVLNGTSLSHLSNGESKTGGSYGTTWGAYGSNYSGDKCAGPNGQANGDTVNATLMQPGINGPMGTNFQVSTNKGGGVWEPGTVNSQSSPWGSGNGANSGGSRRGWGSPAQNTGTSLSSVEWNKLPSNQHSNDSANGNGSKKLTNGWKSTEEDDQGSGTSQTNEQNSVWAKAGGTVESDGSAESTGRLEEKVTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPNSNDTSSVSGWGDPKPTLRWGDSKGSNCQGGWEDDSAATGMIKSNQWGHCKEDKSAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGESSRSNHWGEANKKSSSGGSDSDRSISGWNELGKTSSFTWGNNINPNNSSGWDESSKPNSSQGWGDPPKCNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATSKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNIPEGSSRSDQQAQMHRLLPAASAISSKETSSGSGWGEPWAEPSTPATTVDNGTSAWGKPIDSGPSWGEPITAASSASTWGSNSVGPQSLSKSGPKSMQDGWCGEDTPLPGSRPTGWEEEEDVEIGMWNSSSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEDAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRAVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNNALPNQALGSIAGLGTQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSANRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQPLHQPAMKSFLDNVMPHTTPELQKGPSPVNAFSSFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSMSVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINSLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_008759748.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759748.2

ORF Insert Sequence:

ATGAGAGAGT TGGAAGCTAA AGCTACCAAA GACGTAGAGA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAGGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCTAGTGTAA GCCAGCCTCA GCCTGCCAAC TCCGATAACG GCACTTCCAC AGCTACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AGCAATCAGC AGCCGCCGCC GCCGCAGCAG 
CAGCCGCAGC AGCAGCAGCA GCAGCAGCAG CAACAACAAG AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCCACAA GCCTTGCCTC GGTATCCTCG TGAAGTACCT 
CCACGATTTC GCCACCAGGA ACACAAACAG CTTCTAAAGA GGGGTCAGCA TTTTCCAGTC 
ATAGCAGCAA ACTTGGGATC TGCTGTTAAG GTGTTAAACA GCCAATCAGA AAGCAGTGCT 
GTAACAAATC AACAGCCACA AAATAACGGA GAGGTGCAGA ACAGCAAAAG CCAGTCAGAT 
ATAAATCATA ATACTTCAGG ATCCCATTAT GAAAATTGCC AGCGGGGACC TGTGTCTTCT 
ACAAGTGACT GTAGCACAAG CTGTAAGAAT GCTGTAAACG ACTTGTTGGA AAAAGAAGCA 
TGGCCCTCAG CCCCTGGCAG TGATCCTGAG TTGGCTTCAG AATGTATAGA TGCTGATTCT 
GCCTCCAATT CTGAGTCAGA GAGAAACATC ACTGTCATGG CTTCAGGGAA CACAGGTGGT 
GAGAAAGATG GCCTTCGGAA CAGCACTGGA CTCGGTCCCC AAAGCAAATT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG TCATGGAAGT AGTACTGGGC CATGGGGCTT TCCCCATGGA 
GCCATAATAA GCACATGTCA GGTCTCTGTG GATGCTCCTG AAAGCAAACC AGAAAGTAGT 
AACAGTAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGAAAA CAACGGACTT 
GCCCTAAAAG GGCCTGTAGG GAGTGGGAAT TCTGGCATCA ATATTCAGTG TAGTACCATA 
GGCCAGATGC CTAACAATCA GAGTATCAAC TCTAAAGTCA GTGGCTCTTC TACCCATGGT 
ACCTGGGGTA GCCTTCAGGA AACTTGTGAG CCTGAAGTAA GTGGTTCACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAGAA TATCACCACT GAAACGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAAA ATAATGAACT GCCTACTAGT 
AATCCAGGGG CCTGGCGTGT GAGCACAATG AATCATCCTC AGATACAGGC TCCGTCAGTT 
CTGAATGGCA CTTCCCTTTC TCACCTTAGT AATGGAGAGT CAAAAACTGG AGGCTCCTAC 
GGTACTACAT GGGGTGCCTA TGGTTCTAAT TACTCTGGCG ACAAATGTGC AGGCCCTAAT 
GGCCAAGCCA ATGGTGACAC TGTGAATGCA ACTCTAATGC AGCCTGGCAT AAATGGGCCT 
ATGGGCACTA ACTTTCAAGT TAGTACAAAT AAAGGGGGAG GTGTATGGGA GCCTGGGACA 
GTGAATTCCC AGAGTTCACC ATGGGGAAGT GGAAATGGTG CAAATTCTGG AGGAAGTCGA 
AGAGGATGGG GAAGTCCTGC ACAGAACACT GGCACTAGTC TGTCCAGTGT TGAGTGGAAC 
AAACTGCCTA GCAACCAGCA TTCCAATGAC AGTGCAAATG GCAATGGTAG TAAGAAACTT 
ACAAATGGAT GGAAATCTAC TGAGGAAGAT GATCAGGGTT CTGGCACATC TCAGACGAAT 
GAGCAAAACA GTGTGTGGGC CAAAGCAGGA GGCACAGTGG AGAGTGATGG TAGTGCAGAG 
AGCACTGGAC GCCTTGAAGA AAAAGTAACC GGGGAAAGCC AGAGTAGAGA TAGAAGAAAA 
ATTGATCAGC ACACATTACT CCAAAGCATT GTAAACAGAA CTGACTTAGA TCCACGTGTC 
CTATCCAATT CTGGGTGGGG ACAGACTCCT ATTAAGCAGA ATACTGCCTG GGATACAGAG 
ACATCACCAA GAGGGGAAAG AAAGACTGAC AATGGGACAG AGGCCTGGGG AAGCTCTGCA 
ACACAGACTT TTAACTCAGG GGCATGTACA GATAAGACTA GCCCTAATAG TAATGATACC 
TCATCTGTAT CAGGGTGGGG TGATCCCAAA CCTACTCTGA GGTGGGGAGA TTCCAAAGGC 
TCAAACTGCC AGGGGGGGTG GGAAGATGAT TCTGCTGCTA CAGGAATGAT CAAGAGCAAT 
CAGTGGGGGC ATTGCAAAGA AGACAAGTCT GCATGGAATG ATTCGCAAAA GAACAAACAA 
GGCTGGGGCG ATGGACAAAA GTCAAGCCAA GGTTGGTCCG TTTCTGCCAG TGATAACTGG 
GGAGAATCTT CCAGGAGTAA CCATTGGGGT GAGGCTAATA AGAAATCCAG CTCAGGAGGC 
AGTGACAGCG ACAGGTCCAT TTCTGGTTGG AACGAACTTG GTAAAACTAG TTCTTTTACT 
TGGGGAAATA ATATAAATCC AAATAACTCA TCAGGATGGG ATGAATCTTC TAAACCTAAC 
TCTTCCCAGG GATGGGGAGA CCCTCCAAAG TGTAATCAGT CCCTAGGTTG GGGAGATTCA 
TCAAAACCAG TTAGTTCTCC AGATTGGAAC AAGCAACAAG ACATTGTTGG ATCGTGGGGA 
ATCCCACCAG CCACCAGCAA GCCTCCTGGT ACAGGCTGGC TTGGGGGACC TATTCCTGCT 
CCAGCAAAGG AGGAAGAACC CACAGGCTGG GAGGAACCAT CCCCAGAGTC TATACGACGA 
AAAATGGAGA TCGATGATGG AACTTCAGCT TGGGGAGATC CAAGCAAATA CAACTACAAA 
AATGTGAACA TGTGGAATAA AAACATCCCA GAAGGCAGCA GCCGCTCAGA CCAGCAAGCA 
CAGATGCACC GGTTGTTGCC GGCTGCAAGT GCCATCTCAA GCAAGGAGAC CAGCAGTGGC 
TCTGGCTGGG GTGAGCCTTG GGCAGAGCCT TCTACTCCAG CCACGACTGT GGATAATGGT 
ACTTCAGCCT GGGGCAAGCC CATAGACAGT GGTCCCAGCT GGGGAGAACC CATTACTGCA 
GCATCCAGTG CATCCACGTG GGGCTCCAAC TCTGTTGGTC CACAATCATT AAGCAAATCT 
GGACCCAAAT CTATGCAAGA TGGCTGGTGT GGTGAGGACA CGCCATTGCC TGGAAGTCGC 
CCCACTGGCT GGGAAGAGGA GGAGGATGTA GAGATTGGGA TGTGGAACAG TAGCTCATCT 
CAAGAGCTTA ACTCATCCTT AAACTGGCCA CCATATACCA AGAAAATGTC ATCCAAGGGT 
CTGAGTGGCA AAAAAAGGAG AAGGGAAAGG GGAATGATGA AAGGCGGAAA CAAACAAGAG 
GACGCGTGGA TAAACCCATT CGTTAAGCAG TTTTCAAATA TCAGTTTTTC AAGAGACTCA 
CCAGAAGAAA ATGTACAGAG CAATAAGATG GATCTTTCTG GAGGAATGTT ACAGGACAAG 
CGGATGGAGA TTGATAAGCA TAGCCTGAAT ATTGGTGACT ACAATCGAGC GGTCGGGAAA 
GGCCCTGGGT CTCGGCCTCA GATTTCCAAA GAGTCTTCCA TGGAGCGCAA TCCTTACTTT 
GATAAGGATG GCATTGTAGC AGATGAATCC CAAAACATGC AGTTTATGTC CAGTCAAAGC 
ATGAAGCTTC CCCCTTCAAA TAATGCACTA CCTAACCAGG CCCTTGGCTC CATAGCAGGG 
CTGGGTACGC AAAACTTGAA TTCTGTTAGA CAGAATGGCA ATCCCAGTAT GTTTGGTGTT 
GGAAACACAG CAGCACAACC CCGGGGCATG CAGCAGCCTC CAGCACAACC TCTCAGTTCA 
TCTCAGCCTA ATCTTCGTGC TCAAGTGCCT CCTCCATTAC TCTCCCCTCA GGTTCCAGTT 
TCATTGCTGA AGTATGCACC AAACAACGGT GGCCTGAATC CGCTCTTTGG CCCTCAACAG 
GTAGCCATGC TGAACCAGCT ATCCCAACTA AACCAGCTTT CTCAGATCTC CCAGTTACAG 
CGATTGTTAG CGCAGCAGCA GAGGGCACAG AGTCAGAGAA GCGTGCCTTC TGCTAACCGA 
CAGCAGCAAG ACCAGCAGGG TCGACCTCTT AGTGTACAGC AGCAGATGAT GCAACAGTCT 
CGTCAGCTTG ATCCAAGCCT GCTGGTGAAG CAGCAGACTC CGCCTTCTCA GCAGCCGCTC 
CATCAGCCAG CCATGAAGTC CTTCCTTGAC AATGTCATGC CCCACACTAC ACCCGAGCTA 
CAGAAAGGGC CATCGCCAGT AAACGCTTTC AGCAGCTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCACGACT AAGGAAGTGG 
ACAACAGTGG ACAGCATGTC TGTGAACACG TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTTAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAT 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAACTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAGTCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ATGTTCCCCT CAGCAGTACA GCACAAAGCA CTTCAGCCAG AAATAGTGAT 
TCCAAATTGA CGTGGTCTCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAAGTCCCTT TACCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGGCTGACT 
GGTCAGAAGC CACCCTTGTC TACGTGGGAT AATTCTCCCC TTCGTGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGATA TACCCCAGGC TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACCAATT GGCTTGTTCT GAAGAACCTC ACACCTCAGA TTGACGGCTC CACTCTGCGC 
ACCCTGTGCA TGCAGCATGG CCCACTGATT ACATTCCATC TGAACCTCCC ACATGGAAAT 
GCTCTGGTCC GTTACAGTTC AAAGGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTGCTGG GGAACACTAC TATTCTCGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGC 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCTGGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC TGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCACGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCCC CAAGCAGCAG TGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAATGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnrc6a ( XM_008759749.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757971.1 Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X3, mRNA.

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CloneID	ORa46684
Clone ID Related Accession (Same CDS sequence)	XM_008759749.2
Accession Version	XM_008759749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGAATG ATGAAAGGCG GAAACAAACA AGAGGACGCG 
TGGATAAACC CATTCGTTAA GCAGTTTTCA AATATCAGTT TTTCAAGAGA CTCACCAGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCTGGAGGAA TGTTACAGGA CAAGCGGATG 
GAGATTGATA AGCATAGCCT GAATATTGGT GACTACAATC GAGCGGTCGG GAAAGGCCCT 
GGGTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAATCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAATGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ACGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTCAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAGAGGGC ACAGAGTCAG AGAAGCGTGC CTTCTGCTAA CCGACAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGTGTA CAGCAGCAGA TGATGCAACA GTCTCGTCAG 
CTTGATCCAA GCCTGCTGGT GAAGCAGCAG ACTCCGCCTT CTCAGCAGCC GCTCCATCAG 
CCAGCCATGA AGTCCTTCCT TGACAATGTC ATGCCCCACA CTACACCCGA GCTACAGAAA 
GGGCCATCGC CAGTAAACGC TTTCAGCAGC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAC GACTAAGGAA GTGGACAACA 
GTGGACAGCA TGTCTGTGAA CACGTCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TTAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAATAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA CTGGCCACCA GAATTTCGCC CTGGTGAACC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAGT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAATGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACGTGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAAGTC 
CCTTTACCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGGCT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTG TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGCTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACC 
AATTGGCTTG TTCTGAAGAA CCTCACACCT CAGATTGACG GCTCCACTCT GCGCACCCTG 
TGCATGCAGC ATGGCCCACT GATTACATTC CATCTGAACC TCCCACATGG AAATGCTCTG 
GTCCGTTACA GTTCAAAGGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTG 
CTGGGGAACA CTACTATTCT CGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGCTTCTTT 
GCACAAAGTC AGTCTCTGAC CCCTTCTCCT GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACTGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC CCTCTGGGGG 
ACCCCACATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGTGACCC CCGGGGAATT 
AGCAGCCCAT CCCCCATTAA TGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757971.1
CDS	302..6190
Translation	MQLVAEPGLEARCPGSQDTPLPEEWNRDLVQEEEQLMEEKKRKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSDNGTSTATSTNNNAKRATASNQQPPPPQQQPQQQQQQQQQQQEQQQQQQQQQQQQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSAVTNQQPQNNGEVQNSKSQSDINHNTSGSHYENCQRGPVSSTSDCSTSCKNAVNDLLEKEAWPSAPGSDPELASECIDADSASNSESERNITVMASGNTGGEKDGLRNSTGLGPQSKFVVGSSSNNVGHGSSTGPWGFPHGAIISTCQVSVDAPESKPESSNSRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGNSGINIQCSTIGQMPNNQSINSKVSGSSTHGTWGSLQETCEPEVSGSQKVSFSGQPQNITTETTGPNNTTNFMTSSLPNSGSVQNNELPTSNPGAWRVSTMNHPQIQAPSVLNGTSLSHLSNGESKTGGSYGTTWGAYGSNYSGDKCAGPNGQANGDTVNATLMQPGINGPMGTNFQVSTNKGGGVWEPGTVNSQSSPWGSGNGANSGGSRRGWGSPAQNTGTSLSSVEWNKLPSNQHSNDSANGNGSKKLTNGWKSTEEDDQGSGTSQTNEQNSVWAKAGGTVESDGSAESTGRLEEKVTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPNSNDTSSVSGWGDPKPTLRWGDSKGSNCQGGWEDDSAATGMIKSNQWGHCKEDKSAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGESSRSNHWGEANKKSSSGGSDSDRSISGWNELGKTSSFTWGNNINPNNSSGWDESSKPNSSQGWGDPPKCNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATSKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNIPEGSSRSDQQAQMHRLLPAASAISSKETSSGSGWGEPWAEPSTPATTVDNGTSAWGKPIDSGPSWGEPITAASSASTWGSNSVGPQSLSKSGPKSMQDGWCGEDTPLPGSRPTGWEEEEDVEIGMWNSSSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEDAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRAVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNNALPNQALGSIAGLGTQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSANRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQPLHQPAMKSFLDNVMPHTTPELQKGPSPVNAFSSFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSMSVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINSLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_008759749.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759749.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGAATG ATGAAAGGCG GAAACAAACA AGAGGACGCG 
TGGATAAACC CATTCGTTAA GCAGTTTTCA AATATCAGTT TTTCAAGAGA CTCACCAGAA 
GAAAATGTAC AGAGCAATAA GATGGATCTT TCTGGAGGAA TGTTACAGGA CAAGCGGATG 
GAGATTGATA AGCATAGCCT GAATATTGGT GACTACAATC GAGCGGTCGG GAAAGGCCCT 
GGGTCTCGGC CTCAGATTTC CAAAGAGTCT TCCATGGAGC GCAATCCTTA CTTTGATAAG 
GATGGCATTG TAGCAGATGA ATCCCAAAAC ATGCAGTTTA TGTCCAGTCA AAGCATGAAG 
CTTCCCCCTT CAAATAATGC ACTACCTAAC CAGGCCCTTG GCTCCATAGC AGGGCTGGGT 
ACGCAAAACT TGAATTCTGT TAGACAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTCAG TTCATCTCAG 
CCTAATCTTC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAGAGGGC ACAGAGTCAG AGAAGCGTGC CTTCTGCTAA CCGACAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGTGTA CAGCAGCAGA TGATGCAACA GTCTCGTCAG 
CTTGATCCAA GCCTGCTGGT GAAGCAGCAG ACTCCGCCTT CTCAGCAGCC GCTCCATCAG 
CCAGCCATGA AGTCCTTCCT TGACAATGTC ATGCCCCACA CTACACCCGA GCTACAGAAA 
GGGCCATCGC CAGTAAACGC TTTCAGCAGC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAC GACTAAGGAA GTGGACAACA 
GTGGACAGCA TGTCTGTGAA CACGTCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TTAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAATAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA CTGGCCACCA GAATTTCGCC CTGGTGAACC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAGT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAATGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACGTGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAAGTC 
CCTTTACCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGGCT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTG TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGCTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACC 
AATTGGCTTG TTCTGAAGAA CCTCACACCT CAGATTGACG GCTCCACTCT GCGCACCCTG 
TGCATGCAGC ATGGCCCACT GATTACATTC CATCTGAACC TCCCACATGG AAATGCTCTG 
GTCCGTTACA GTTCAAAGGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTG 
CTGGGGAACA CTACTATTCT CGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGCTTCTTT 
GCACAAAGTC AGTCTCTGAC CCCTTCTCCT GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACTGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC CCTCTGGGGG 
ACCCCACATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGTGACCC CCGGGGAATT 
AGCAGCCCAT CCCCCATTAA TGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnrc6a ( XM_017589208.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444697.1 Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X4, mRNA.

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CloneID	ORa56612
Clone ID Related Accession (Same CDS sequence)	XM_017589208.1
Accession Version	XM_017589208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	trinucleotide repeat-containing gene 6A protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGTCTG AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA 
ATGATGAAAG GCGGAAACAA ACAAGAGGAC GCGTGGATAA ACCCATTCGT TAAGCAGTTT 
TCAAATATCA GTTTTTCAAG AGACTCACCA GAAGAAAATG TACAGAGCAA TAAGATGGAT 
CTTTCTGGAG GAATGTTACA GGACAAGCGG ATGGAGATTG ATAAGCATAG CCTGAATATT 
GGTGACTACA ATCGAGCGGT CGGGAAAGGC CCTGGGTCTC GGCCTCAGAT TTCCAAAGAG 
TCTTCCATGG AGCGCAATCC TTACTTTGAT AAGGATGGCA TTGTAGCAGA TGAATCCCAA 
AACATGCAGT TTATGTCCAG TCAAAGCATG AAGCTTCCCC CTTCAAATAA TGCACTACCT 
AACCAGGCCC TTGGCTCCAT AGCAGGGCTG GGTACGCAAA ACTTGAATTC TGTTAGACAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT CAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAGA GGGCACAGAG TCAGAGAAGC 
GTGCCTTCTG CTAACCGACA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTACAGCAG 
CAGATGATGC AACAGTCTCG TCAGCTTGAT CCAAGCCTGC TGGTGAAGCA GCAGACTCCG 
CCTTCTCAGC AGCCGCTCCA TCAGCCAGCC ATGAAGTCCT TCCTTGACAA TGTCATGCCC 
CACACTACAC CCGAGCTACA GAAAGGGCCA TCGCCAGTAA ACGCTTTCAG CAGCTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCACGACTAA GGAAGTGGAC AACAGTGGAC AGCATGTCTG TGAACACGTC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTTAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAATAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAACTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAGTCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAT GTTCCCCTCA GCAGTACAGC ACAAAGCACT 
TCAGCCAGAA ATAGTGATTC CAAATTGACG TGGTCTCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA AGTCCCTTTA CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GGCTGACTGG TCAGAAGCCA CCCTTGTCTA CGTGGGATAA TTCTCCCCTT 
CGTGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGATATA CCCCAGGCTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACCAATTGG CTTGTTCTGA AGAACCTCAC ACCTCAGATT 
GACGGCTCCA CTCTGCGCAC CCTGTGCATG CAGCATGGCC CACTGATTAC ATTCCATCTG 
AACCTCCCAC ATGGAAATGC TCTGGTCCGT TACAGTTCAA AGGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTGCTGGGG AACACTACTA TTCTCGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGCTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCTGGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACTG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCACGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCCCCA 
AGCAGCAGTG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAATGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444697.1
CDS	302..6154
Translation	MQLVAEPGLEARCPGSQDTPLPEEWNRDLVQEEEQLMEEKKRKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSDNGTSTATSTNNNAKRATASNQQPPPPQQQPQQQQQQQQQQQEQQQQQQQQQQQQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSAVTNQQPQNNGEVQNSKSQSDINHNTSGSHYENCQRGPVSSTSDCSTSCKNAVNDLLEKEAWPSAPGSDPELASECIDADSASNSESERNITVMASGNTGGEKDGLRNSTGLGPQSKFVVGSSSNNVGHGSSTGPWGFPHGAIISTCQVSVDAPESKPESSNSRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGNSGINIQCSTIGQMPNNQSINSKVSGSSTHGTWGSLQETCEPEVSGSQKVSFSGQPQNITTETTGPNNTTNFMTSSLPNSGSVQNNELPTSNPGAWRVSTMNHPQIQAPSVLNGTSLSHLSNGESKTGGSYGTTWGAYGSNYSGDKCAGPNGQANGDTVNATLMQPGINGPMGTNFQVSTNKGGGVWEPGTVNSQSSPWGSGNGANSGGSRRGWGSPAQNTGTSLSSVEWNKLPSNQHSNDSANGNGSKKLTNGWKSTEEDDQGSGTSQTNEQNSVWAKAGGTVESDGSAESTGRLEEKVTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACTDKTSPNSNDTSSVSGWGDPKPTLRWGDSKGSNCQGGWEDDSAATGMIKSNQWGHCKEDKSAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGESSRSNHWGEANKKSSSGGSDSDRSISGWNELGKTSSFTWGNNINPNNSSGWDESSKPNSSQGWGDPPKCNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATSKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNIPEGSSRSDQQAQMHRLLPAASAISSKETSSGSGWGEPWAEPSTPATTVDNGTSAWGKPIDSGPSWGEPITAASSASTWGSNSVGPQSLSKSGPKSMQDGWCGEDTPLPGSRPTGWEEEEDVEIGMWNSSSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEDAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRAVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNNALPNQALGSIAGLGTQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSANRQQQDQQGRPLSVQQQMMQQSRQLDPSLLVKQQTPPSQQPLHQPAMKSFLDNVMPHTTPELQKGPSPVNAFSSFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSMSVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINSLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017589208.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus trinucleotide repeat containing 6a (Tnrc6a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589208.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAACC AGGCCTAGAA GCCAGGTGTC CTGGATCTCA AGATACCCCA 
CTGCCTGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAGGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCT AGTGTAAGCC AGCCTCAGCC TGCCAACTCC 
GATAACGGCA CTTCCACAGC TACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAGC 
AATCAGCAGC CGCCGCCGCC GCAGCAGCAG CCGCAGCAGC AGCAGCAGCA GCAGCAGCAA 
CAACAAGAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCCACAAGCC 
TTGCCTCGGT ATCCTCGTGA AGTACCTCCA CGATTTCGCC ACCAGGAACA CAAACAGCTT 
CTAAAGAGGG GTCAGCATTT TCCAGTCATA GCAGCAAACT TGGGATCTGC TGTTAAGGTG 
TTAAACAGCC AATCAGAAAG CAGTGCTGTA ACAAATCAAC AGCCACAAAA TAACGGAGAG 
GTGCAGAACA GCAAAAGCCA GTCAGATATA AATCATAATA CTTCAGGATC CCATTATGAA 
AATTGCCAGC GGGGACCTGT GTCTTCTACA AGTGACTGTA GCACAAGCTG TAAGAATGCT 
GTAAACGACT TGTTGGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCTGAGTTG 
GCTTCAGAAT GTATAGATGC TGATTCTGCC TCCAATTCTG AGTCAGAGAG AAACATCACT 
GTCATGGCTT CAGGGAACAC AGGTGGTGAG AAAGATGGCC TTCGGAACAG CACTGGACTC 
GGTCCCCAAA GCAAATTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGTCA TGGAAGTAGT 
ACTGGGCCAT GGGGCTTTCC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT 
GCTCCTGAAA GCAAACCAGA AAGTAGTAAC AGTAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAAAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGGAATTCT 
GGCATCAATA TTCAGTGTAG TACCATAGGC CAGATGCCTA ACAATCAGAG TATCAACTCT 
AAAGTCAGTG GCTCTTCTAC CCATGGTACC TGGGGTAGCC TTCAGGAAAC TTGTGAGCCT 
GAAGTAAGTG GTTCACAGAA GGTTTCATTC AGTGGTCAAC CTCAGAATAT CACCACTGAA 
ACGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAAAATA ATGAACTGCC TACTAGTAAT CCAGGGGCCT GGCGTGTGAG CACAATGAAT 
CATCCTCAGA TACAGGCTCC GTCAGTTCTG AATGGCACTT CCCTTTCTCA CCTTAGTAAT 
GGAGAGTCAA AAACTGGAGG CTCCTACGGT ACTACATGGG GTGCCTATGG TTCTAATTAC 
TCTGGCGACA AATGTGCAGG CCCTAATGGC CAAGCCAATG GTGACACTGT GAATGCAACT 
CTAATGCAGC CTGGCATAAA TGGGCCTATG GGCACTAACT TTCAAGTTAG TACAAATAAA 
GGGGGAGGTG TATGGGAGCC TGGGACAGTG AATTCCCAGA GTTCACCATG GGGAAGTGGA 
AATGGTGCAA ATTCTGGAGG AAGTCGAAGA GGATGGGGAA GTCCTGCACA GAACACTGGC 
ACTAGTCTGT CCAGTGTTGA GTGGAACAAA CTGCCTAGCA ACCAGCATTC CAATGACAGT 
GCAAATGGCA ATGGTAGTAA GAAACTTACA AATGGATGGA AATCTACTGA GGAAGATGAT 
CAGGGTTCTG GCACATCTCA GACGAATGAG CAAAACAGTG TGTGGGCCAA AGCAGGAGGC 
ACAGTGGAGA GTGATGGTAG TGCAGAGAGC ACTGGACGCC TTGAAGAAAA AGTAACCGGG 
GAAAGCCAGA GTAGAGATAG AAGAAAAATT GATCAGCACA CATTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTA TCCAATTCTG GGTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAGACA TCACCAAGAG GGGAAAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC ATGTACAGAT 
AAGACTAGCC CTAATAGTAA TGATACCTCA TCTGTATCAG GGTGGGGTGA TCCCAAACCT 
ACTCTGAGGT GGGGAGATTC CAAAGGCTCA AACTGCCAGG GGGGGTGGGA AGATGATTCT 
GCTGCTACAG GAATGATCAA GAGCAATCAG TGGGGGCATT GCAAAGAAGA CAAGTCTGCA 
TGGAATGATT CGCAAAAGAA CAAACAAGGC TGGGGCGATG GACAAAAGTC AAGCCAAGGT 
TGGTCCGTTT CTGCCAGTGA TAACTGGGGA GAATCTTCCA GGAGTAACCA TTGGGGTGAG 
GCTAATAAGA AATCCAGCTC AGGAGGCAGT GACAGCGACA GGTCCATTTC TGGTTGGAAC 
GAACTTGGTA AAACTAGTTC TTTTACTTGG GGAAATAATA TAAATCCAAA TAACTCATCA 
GGATGGGATG AATCTTCTAA ACCTAACTCT TCCCAGGGAT GGGGAGACCC TCCAAAGTGT 
AATCAGTCCC TAGGTTGGGG AGATTCATCA AAACCAGTTA GTTCTCCAGA TTGGAACAAG 
CAACAAGACA TTGTTGGATC GTGGGGAATC CCACCAGCCA CCAGCAAGCC TCCTGGTACA 
GGCTGGCTTG GGGGACCTAT TCCTGCTCCA GCAAAGGAGG AAGAACCCAC AGGCTGGGAG 
GAACCATCCC CAGAGTCTAT ACGACGAAAA ATGGAGATCG ATGATGGAAC TTCAGCTTGG 
GGAGATCCAA GCAAATACAA CTACAAAAAT GTGAACATGT GGAATAAAAA CATCCCAGAA 
GGCAGCAGCC GCTCAGACCA GCAAGCACAG ATGCACCGGT TGTTGCCGGC TGCAAGTGCC 
ATCTCAAGCA AGGAGACCAG CAGTGGCTCT GGCTGGGGTG AGCCTTGGGC AGAGCCTTCT 
ACTCCAGCCA CGACTGTGGA TAATGGTACT TCAGCCTGGG GCAAGCCCAT AGACAGTGGT 
CCCAGCTGGG GAGAACCCAT TACTGCAGCA TCCAGTGCAT CCACGTGGGG CTCCAACTCT 
GTTGGTCCAC AATCATTAAG CAAATCTGGA CCCAAATCTA TGCAAGATGG CTGGTGTGGT 
GAGGACACGC CATTGCCTGG AAGTCGCCCC ACTGGCTGGG AAGAGGAGGA GGATGTAGAG 
ATTGGGATGT GGAACAGTAG CTCATCTCAA GAGCTTAACT CATCCTTAAA CTGGCCACCA 
TATACCAAGA AAATGTCATC CAAGGGTCTG AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA 
ATGATGAAAG GCGGAAACAA ACAAGAGGAC GCGTGGATAA ACCCATTCGT TAAGCAGTTT 
TCAAATATCA GTTTTTCAAG AGACTCACCA GAAGAAAATG TACAGAGCAA TAAGATGGAT 
CTTTCTGGAG GAATGTTACA GGACAAGCGG ATGGAGATTG ATAAGCATAG CCTGAATATT 
GGTGACTACA ATCGAGCGGT CGGGAAAGGC CCTGGGTCTC GGCCTCAGAT TTCCAAAGAG 
TCTTCCATGG AGCGCAATCC TTACTTTGAT AAGGATGGCA TTGTAGCAGA TGAATCCCAA 
AACATGCAGT TTATGTCCAG TCAAAGCATG AAGCTTCCCC CTTCAAATAA TGCACTACCT 
AACCAGGCCC TTGGCTCCAT AGCAGGGCTG GGTACGCAAA ACTTGAATTC TGTTAGACAG 
AATGGCAATC CCAGTATGTT TGGTGTTGGA AACACAGCAG CACAACCCCG GGGCATGCAG 
CAGCCTCCAG CACAACCTCT CAGTTCATCT CAGCCTAATC TTCGTGCTCA AGTGCCTCCT 
CCATTACTCT CCCCTCAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAGA GGGCACAGAG TCAGAGAAGC 
GTGCCTTCTG CTAACCGACA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTACAGCAG 
CAGATGATGC AACAGTCTCG TCAGCTTGAT CCAAGCCTGC TGGTGAAGCA GCAGACTCCG 
CCTTCTCAGC AGCCGCTCCA TCAGCCAGCC ATGAAGTCCT TCCTTGACAA TGTCATGCCC 
CACACTACAC CCGAGCTACA GAAAGGGCCA TCGCCAGTAA ACGCTTTCAG CAGCTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCACGACTAA GGAAGTGGAC AACAGTGGAC AGCATGTCTG TGAACACGTC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTTAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAATAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAACTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAGTCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAT GTTCCCCTCA GCAGTACAGC ACAAAGCACT 
TCAGCCAGAA ATAGTGATTC CAAATTGACG TGGTCTCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA AGTCCCTTTA CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GGCTGACTGG TCAGAAGCCA CCCTTGTCTA CGTGGGATAA TTCTCCCCTT 
CGTGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGATATA CCCCAGGCTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACCAATTGG CTTGTTCTGA AGAACCTCAC ACCTCAGATT 
GACGGCTCCA CTCTGCGCAC CCTGTGCATG CAGCATGGCC CACTGATTAC ATTCCATCTG 
AACCTCCCAC ATGGAAATGC TCTGGTCCGT TACAGTTCAA AGGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTGCTGGGG AACACTACTA TTCTCGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGCTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCTGGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACTG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCACGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCCCCA 
AGCAGCAGTG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAATGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets.
Nucleic acids research41(2)978-94(2013 Jan)
Huntzinger E,Kuzuoglu-?zt?rk D,Braun JE,Eulalio A,Wohlbold L,Izaurralde E

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.
Proceedings of the National Academy of Sciences of the United States of America107(28)12499-504(2010 Jul)
James V,Zhang Y,Foxler DE,de Moor CH,Kong YW,Webb TM,Self TJ,Feng Y,Lagos D,Chu CY,Rana TM,Morley SJ,Longmore GD,Bushell M,Sharp TV

Argonaute proteins at a glance.
Journal of cell science123(Pt 11)1819-23(2010 Jun)
Ender C,Meister G

Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system.
Genes & development21(15)1857-62(2007 Aug)
Wakiyama M,Takimoto K,Ohara O,Yokoyama S

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Tnrc6a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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