Phf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phf2
Entrez Gene ID	306814
Full Name	PHD finger protein 2
General protein information	Preferred Names PHD finger protein 2 Names lysine-specific demethylase PHF2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14

mRNA and Protein(s)

mRNA	Protein	Name
XM_006253729.2	XP_006253791.1	lysine-specific demethylase PHF2 isoform X1
NM_001107342.1	NP_001100812.1	lysine-specific demethylase PHF2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHF2	XP_003312232.2
Bos taurus (cattle)	PHF2	XP_002689896.2
Gallus gallus (chicken)	PHF2	XP_414324.2
Xenopus tropicalis (tropical clawed frog)	phf2	XP_002943074.2
	LOC717585	XP_002808154.1
Canis lupus familiaris (dog)	PHF2	XP_005616040.1
Danio rerio (zebrafish)	phf2	NP_001189347.1
Mus musculus (house mouse)	Phf2	NP_035208.2
Rattus norvegicus (Norway rat)	Phf2	NP_001100812.1
Homo sapiens (human)	PHF2	NP_005383.3

Gene Ontology
Bibliography

Related articles in PubMed

Histone lysine methylation dynamics: establishment, regulation, and biological impact.
Black JC, Van Rechem C, Whetstine JR
Molecular cell48(4)491-507(2012 Nov)

PKA-dependent regulation of the histone lysine demethylase complex PHF2-ARID5B.
Baba A, Ohtake F, Okuno Y, Yokota K, Okada M, Imai Y, Ni M, Meyer CA, Igarashi K, Kanno J, Brown M, Kato S
Nature cell biology13(6)668-75(2011 Jun)

Structural basis for human PHF2 Jumonji domain interaction with metal ions.
Horton JR, Upadhyay AK, Hashimoto H, Zhang X, Cheng X
Journal of molecular biology406(1)1-8(2011 Feb)

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Ohta S, Bukowski-Wills JC, Sanchez-Pulido L, Alves Fde L, Wood L, Chen ZA, Platani M, Fischer L, Hudson DF, Ponting CP, Fukagawa T, Earnshaw WC, Rappsilber J
Cell142(5)810-21(2010 Sep)

Recognition of histone H3K4 trimethylation by the plant homeodomain of PHF2 modulates histone demethylation.
Wen H, Li J, Song T, Lu M, Kan PY, Lee MG, Sha B, Shi X
The Journal of biological chemistry285(13)9322-6(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Phf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa41520	NM_001107342.1 Latest version!	Rattus norvegicus PHD finger protein 2 (Phf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa37768	XM_006253729.2 Latest version!	Rattus norvegicus PHD finger protein 2 (Phf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phf2 ( NM_001107342.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100812.1 Rattus norvegicus PHD finger protein 2 (Phf2), mRNA.

Price & Availability↑

CloneID	ORa41520
Clone ID Related Accession (Same CDS sequence)	NM_001107342.1
Accession Version	NM_001107342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	lysine-specific demethylase PHF2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473977.1. On or before Oct 4, 2007 this sequence version replaced XM_001056853.1, XM_225206.4.

ATGGAGGAGC ATGGCTTCAC CGAGCCCATC CTTGTCCCCA AGAAAGATGG CCTGGGCTTG 
GCTGTCCCTG CCCCAACATT CTACGTGAGT GACGTGGAGA ACTATGTGGG GCCAGAGAGG 
AGTGTGGACG TGACAGACGT TACCAAGCAG AAAGACTGCA AAATGAAGCT GAAAGAATTC 
GTGGACTATT ACTACAGCAC CAACCGCAAA CGTGTTCTCA ATGTGACCAA CCTCGAGTTC 
TCAGACACCA GAATGTCCAG CTTTGTGGAG CCACCTGACA TTGTGAAGAA ACTATCGTGG 
GTGGAAAACT ACTGGCCCGA TGACGCGCTG CTGGCCAAGC CCAAGGTGAC CAAGTACTGC 
CTGATCTGTG TGAAGGACAG CTACACCGAC TTCCACATTG ATTCTGGAGG TGCCTCAGCC 
TGGTACCACG TTCTCAAGGG GGAGAAGATC TTCTATCTCA TCCGGCCGGC CTCAGCCAAC 
CTCTCCCTAT ATGAATGCTG GCGGTCAGCC TCCAACCACA GCGAGATGTT CTTTGCTGAC 
CAGGTGGACA AGTGCTATAA GTGCACTGTC AAGCAGGGCC AGACCCTCTT CATCCCCTCA 
GGCTGGATAT ACGCTACGCT CACCCCTGTG GACTGCCTGG CCTTCGCTGG ACATTTTCTT 
CACAGCCTCA GTGTGGAGAT GCAGATGAGG GCATACGAGG TGGAAAGAAG GTTAAAACTA 
GGCAGCCTGA CTCAATTTCC CAACTTTGAA ACTGCCTGCT GGTACATGGG GAAGCATCTA 
TTGGAAGCAT TCAAAGGTTC TCACAAGTCT GGGAAACAGC TGCCCCCACA CTTAGTTCAA 
GGAGCTAAGA TTCTCAATGG TGCTTTTAGG TCATGGACGA AGAAGCAGGC TTTGGCAGAG 
CACGAGGACG AGCTCCCGGA GCACTTCAGG CCCTCACAGC TCATCAAGGA CCTGGCCAAA 
GAGATCAGGC TCAGCGAGAA TGCCTCCAAA ACTGTCCGAC CTGAAGTAAA TGCTGTCGCC 
TCCTCAGATG AGGTCTGTGA CGGGGACCGG GAGAAGGAGG AACCGCCATC TCCCATTGAG 
TCTACCCCAC CTCGATCCCT CCTGGAGAAA GTGTCCAAAA AAAAGACTTC CAAGACTGTG 
AAGATGCCCA AGCCATCCAA AACCCCCAAG CCCCCCAAGC CCCCCAAGCC CCCCAAAACC 
CTGAAACTCA AGGACGGAAG CAAGAAGAAA GGGAAGAAGT GCAAAGAGTC CGCCTCTCCC 
ACCATCCCCA ACTTGGACCT GCTTGAGGCC CACACCAAGG AGGCGCTGAC CAAGATGGAG 
CCACCGAAGA AAGGAAAGAC TCCAAAGAGC GTCCTGAGTG TTCCCAATAA GGACACGGTC 
CACACGCAGA ATGACGTGGA AAGACTGGAA ATCCGAGAGC AAACAAAGAG CAAGTCGGAG 
GCCAAGTGGA AGTACAAGAA CAGCAAACCT GACTCTTTAC TGAGAATGGA GGAGGAGCAG 
AGGTTGGAGA AGTCACCCCT GGCTGGGAAC AAGGACAAGT TTTCCTTTTC TTTCTCTAAC 
AGAAAACTCC TGGGCTCCAA GCCCCTCAGG CCGCCGAGCA GCCCTGGCGT GTTCGGGACC 
TTGCAGAGCT TCAAGGAGGA CAAGGCCAAG CCCGTCCGAG ATGAGTATGA ATATGTATCG 
GACGACGGGG AGCTGAAGAT TGACGAGTTT CCTATCAGGA GGAAGAAGAA CCCCCCCAAA 
AGGGATTTGT CCTTTTTGTT AGACAAGAAG GAGACTCTCC TCATCCCCAC CTCGAAGCCA 
AAGCTGGACT CCACGGCATA CAAGAGCGAC GACTCCTCTG ATGAGGGCTC TCTGCATATC 
GACACGGACA CCAAGCCAAG CCGAAATGCC AAAGTGAAGA AGGAAAGTGG GAGCTCGGCG 
GCAGGCATCC TGGACCTACT GCAGGCCAGC GAGGAGGTCG GGGCTCTTGA GTACAACCCC 
AACAGCCAGC CCCCTGCCTC CCCCAGTACG CAGGAAGCCA TTCAGGGAAT GTTGTCCATG 
GCCAATCTGC AGGCCTCTGA CTCCTGCCTG CAGACCACAT GGGGCACTGG CCAGGCCAAG 
GGGGGTTCCC TGGCAGCCCA TGGCGCCCGG AAGAATGGTG GTAGCAGCAA AGGGACAGGC 
AAGCGCCTAC TGAAGAGGAC TGCCAAGAAC AGCGTGGATC TTGAGGACTA TGAGGAGCAG 
GACCACCTGG ACGCCTGCTT CAAGGACTCA GACTATGTTT ACCCCTCACT GGAGTCTGAC 
GAAGATAACC CTGTCTTCAA GTCCCGGTCA AAGAAGAGGA AAGGCTCAGA CGATGCTCCT 
TACAGCCCCA CAGCCAGGGT CGGTCCATCA GTGCCGAGAC AAGACAGGCC TGTGCGTGAG 
GGGACCAGAG TGGCCTCCAT TGAGACTGGG CTGGCAGCTG CTGCAGCCAA GCTGTCCCAG 
CAGGAGGAGC AGAAAAACAG GAAGAAGAAG AACACCAAAA GGAAGCCGAC TCCTAACACT 
GCCTCCCCCT CCGTCTCCAC GTCTGCCTCC GCCTCCACGG GTACCACCTC GGCCTCCACC 
ACCCCAGCCT CCACCACCCC GGCCTCCACC ACCCCAGCAT CCACCACCCC GGCCTCTACC 
AGCACAGCCA GCAGCCAGGC CTCACAGGAG GGCAGCTCAC CCGAGCCCCC ACCTGAGTCA 
CACAGCAGTG GCCTAGCTGA CCACGAGTAT ACAGCAGCCG GCACATTCGC AGGGGCCCAG 
GCTGGCCGTG CCTCCCAGCC CATGGCCCCT GGAGTCTTTC TCACACAGAG GCGGCCTTCT 
GCATCATCCC CCAACAACAC TGCTGCCAAA GGAAAACGTA CAAAAAAGGG CATGGCCACC 
GCCAAGCAAA GGCTTGGAAA GATCTTGAAG ATCCATCGGA ATGGGAAACT GCTCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100812.1
CDS	150..3089
Translation	MEEHGFTEPILVPKKDGLGLAVPAPTFYVSDVENYVGPERSVDVTDVTKQKDCKMKLKEFVDYYYSTNRKRVLNVTNLEFSDTRMSSFVEPPDIVKKLSWVENYWPDDALLAKPKVTKYCLICVKDSYTDFHIDSGGASAWYHVLKGEKIFYLIRPASANLSLYECWRSASNHSEMFFADQVDKCYKCTVKQGQTLFIPSGWIYATLTPVDCLAFAGHFLHSLSVEMQMRAYEVERRLKLGSLTQFPNFETACWYMGKHLLEAFKGSHKSGKQLPPHLVQGAKILNGAFRSWTKKQALAEHEDELPEHFRPSQLIKDLAKEIRLSENASKTVRPEVNAVASSDEVCDGDREKEEPPSPIESTPPRSLLEKVSKKKTSKTVKMPKPSKTPKPPKPPKPPKTLKLKDGSKKKGKKCKESASPTIPNLDLLEAHTKEALTKMEPPKKGKTPKSVLSVPNKDTVHTQNDVERLEIREQTKSKSEAKWKYKNSKPDSLLRMEEEQRLEKSPLAGNKDKFSFSFSNRKLLGSKPLRPPSSPGVFGTLQSFKEDKAKPVRDEYEYVSDDGELKIDEFPIRRKKNPPKRDLSFLLDKKETLLIPTSKPKLDSTAYKSDDSSDEGSLHIDTDTKPSRNAKVKKESGSSAAGILDLLQASEEVGALEYNPNSQPPASPSTQEAIQGMLSMANLQASDSCLQTTWGTGQAKGGSLAAHGARKNGGSSKGTGKRLLKRTAKNSVDLEDYEEQDHLDACFKDSDYVYPSLESDEDNPVFKSRSKKRKGSDDAPYSPTARVGPSVPRQDRPVREGTRVASIETGLAAAAAKLSQQEEQKNRKKKNTKRKPTPNTASPSVSTSASASTGTTSASTTPASTTPASTTPASTTPASTSTASSQASQEGSSPEPPPESHSSGLADHEYTAAGTFAGAQAGRASQPMAPGVFLTQRRPSASSPNNTAAKGKRTKKGMATAKQRLGKILKIHRNGKLLL

Target ORF information:

RefSeq Version	NM_001107342.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 2 (Phf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107342.1

ORF Insert Sequence:

ATGGAGGAGC ATGGCTTCAC CGAGCCCATC CTTGTCCCCA AGAAAGATGG CCTGGGCTTG 
GCTGTCCCTG CCCCAACATT CTACGTGAGT GACGTGGAGA ACTATGTGGG GCCAGAGAGG 
AGTGTGGACG TGACAGACGT TACCAAGCAG AAAGACTGCA AAATGAAGCT GAAAGAATTC 
GTGGACTATT ACTACAGCAC CAACCGCAAA CGTGTTCTCA ATGTGACCAA CCTCGAGTTC 
TCAGACACCA GAATGTCCAG CTTTGTGGAG CCACCTGACA TTGTGAAGAA ACTATCGTGG 
GTGGAAAACT ACTGGCCCGA TGACGCGCTG CTGGCCAAGC CCAAGGTGAC CAAGTACTGC 
CTGATCTGTG TGAAGGACAG CTACACCGAC TTCCACATTG ATTCTGGAGG TGCCTCAGCC 
TGGTACCACG TTCTCAAGGG GGAGAAGATC TTCTATCTCA TCCGGCCGGC CTCAGCCAAC 
CTCTCCCTAT ATGAATGCTG GCGGTCAGCC TCCAACCACA GCGAGATGTT CTTTGCTGAC 
CAGGTGGACA AGTGCTATAA GTGCACTGTC AAGCAGGGCC AGACCCTCTT CATCCCCTCA 
GGCTGGATAT ACGCTACGCT CACCCCTGTG GACTGCCTGG CCTTCGCTGG ACATTTTCTT 
CACAGCCTCA GTGTGGAGAT GCAGATGAGG GCATACGAGG TGGAAAGAAG GTTAAAACTA 
GGCAGCCTGA CTCAATTTCC CAACTTTGAA ACTGCCTGCT GGTACATGGG GAAGCATCTA 
TTGGAAGCAT TCAAAGGTTC TCACAAGTCT GGGAAACAGC TGCCCCCACA CTTAGTTCAA 
GGAGCTAAGA TTCTCAATGG TGCTTTTAGG TCATGGACGA AGAAGCAGGC TTTGGCAGAG 
CACGAGGACG AGCTCCCGGA GCACTTCAGG CCCTCACAGC TCATCAAGGA CCTGGCCAAA 
GAGATCAGGC TCAGCGAGAA TGCCTCCAAA ACTGTCCGAC CTGAAGTAAA TGCTGTCGCC 
TCCTCAGATG AGGTCTGTGA CGGGGACCGG GAGAAGGAGG AACCGCCATC TCCCATTGAG 
TCTACCCCAC CTCGATCCCT CCTGGAGAAA GTGTCCAAAA AAAAGACTTC CAAGACTGTG 
AAGATGCCCA AGCCATCCAA AACCCCCAAG CCCCCCAAGC CCCCCAAGCC CCCCAAAACC 
CTGAAACTCA AGGACGGAAG CAAGAAGAAA GGGAAGAAGT GCAAAGAGTC CGCCTCTCCC 
ACCATCCCCA ACTTGGACCT GCTTGAGGCC CACACCAAGG AGGCGCTGAC CAAGATGGAG 
CCACCGAAGA AAGGAAAGAC TCCAAAGAGC GTCCTGAGTG TTCCCAATAA GGACACGGTC 
CACACGCAGA ATGACGTGGA AAGACTGGAA ATCCGAGAGC AAACAAAGAG CAAGTCGGAG 
GCCAAGTGGA AGTACAAGAA CAGCAAACCT GACTCTTTAC TGAGAATGGA GGAGGAGCAG 
AGGTTGGAGA AGTCACCCCT GGCTGGGAAC AAGGACAAGT TTTCCTTTTC TTTCTCTAAC 
AGAAAACTCC TGGGCTCCAA GCCCCTCAGG CCGCCGAGCA GCCCTGGCGT GTTCGGGACC 
TTGCAGAGCT TCAAGGAGGA CAAGGCCAAG CCCGTCCGAG ATGAGTATGA ATATGTATCG 
GACGACGGGG AGCTGAAGAT TGACGAGTTT CCTATCAGGA GGAAGAAGAA CCCCCCCAAA 
AGGGATTTGT CCTTTTTGTT AGACAAGAAG GAGACTCTCC TCATCCCCAC CTCGAAGCCA 
AAGCTGGACT CCACGGCATA CAAGAGCGAC GACTCCTCTG ATGAGGGCTC TCTGCATATC 
GACACGGACA CCAAGCCAAG CCGAAATGCC AAAGTGAAGA AGGAAAGTGG GAGCTCGGCG 
GCAGGCATCC TGGACCTACT GCAGGCCAGC GAGGAGGTCG GGGCTCTTGA GTACAACCCC 
AACAGCCAGC CCCCTGCCTC CCCCAGTACG CAGGAAGCCA TTCAGGGAAT GTTGTCCATG 
GCCAATCTGC AGGCCTCTGA CTCCTGCCTG CAGACCACAT GGGGCACTGG CCAGGCCAAG 
GGGGGTTCCC TGGCAGCCCA TGGCGCCCGG AAGAATGGTG GTAGCAGCAA AGGGACAGGC 
AAGCGCCTAC TGAAGAGGAC TGCCAAGAAC AGCGTGGATC TTGAGGACTA TGAGGAGCAG 
GACCACCTGG ACGCCTGCTT CAAGGACTCA GACTATGTTT ACCCCTCACT GGAGTCTGAC 
GAAGATAACC CTGTCTTCAA GTCCCGGTCA AAGAAGAGGA AAGGCTCAGA CGATGCTCCT 
TACAGCCCCA CAGCCAGGGT CGGTCCATCA GTGCCGAGAC AAGACAGGCC TGTGCGTGAG 
GGGACCAGAG TGGCCTCCAT TGAGACTGGG CTGGCAGCTG CTGCAGCCAA GCTGTCCCAG 
CAGGAGGAGC AGAAAAACAG GAAGAAGAAG AACACCAAAA GGAAGCCGAC TCCTAACACT 
GCCTCCCCCT CCGTCTCCAC GTCTGCCTCC GCCTCCACGG GTACCACCTC GGCCTCCACC 
ACCCCAGCCT CCACCACCCC GGCCTCCACC ACCCCAGCAT CCACCACCCC GGCCTCTACC 
AGCACAGCCA GCAGCCAGGC CTCACAGGAG GGCAGCTCAC CCGAGCCCCC ACCTGAGTCA 
CACAGCAGTG GCCTAGCTGA CCACGAGTAT ACAGCAGCCG GCACATTCGC AGGGGCCCAG 
GCTGGCCGTG CCTCCCAGCC CATGGCCCCT GGAGTCTTTC TCACACAGAG GCGGCCTTCT 
GCATCATCCC CCAACAACAC TGCTGCCAAA GGAAAACGTA CAAAAAAGGG CATGGCCACC 
GCCAAGCAAA GGCTTGGAAA GATCTTGAAG ATCCATCGGA ATGGGAAACT GCTCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf2 ( XM_006253729.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253791.1 Rattus norvegicus PHD finger protein 2 (Phf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa37768
Clone ID Related Accession (Same CDS sequence)	XM_006253729.2
Accession Version	XM_006253729.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lysine-specific demethylase PHF2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006253729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCAG TGCAGAATGG CAGCCAGCTG TTCATCAAGG AGCTGCGGAG CCGAACCTTC 
CCCAGTGCTG AAGATGTGGT GTCCCGTGTA CCAGGCAGCC AGCTCACTGT GGGCTACATG 
GAGGAGCATG GCTTCACCGA GCCCATCCTT GTCCCCAAGA AAGATGGCCT GGGCTTGGCT 
GTCCCTGCCC CAACATTCTA CGTGAGTGAC GTGGAGAACT ATGTGGGGCC AGAGAGGAGT 
GTGGACGTGA CAGACGTTAC CAAGCAGAAA GACTGCAAAA TGAAGCTGAA AGAATTCGTG 
GACTATTACT ACAGCACCAA CCGCAAACGT GTTCTCAATG TGACCAACCT CGAGTTCTCA 
GACACCAGAA TGTCCAGCTT TGTGGAGCCA CCTGACATTG TGAAGAAACT ATCGTGGGTG 
GAAAACTACT GGCCCGATGA CGCGCTGCTG GCCAAGCCCA AGGTGACCAA GTACTGCCTG 
ATCTGTGTGA AGGACAGCTA CACCGACTTC CACATTGATT CTGGAGGTGC CTCAGCCTGG 
TACCACGTTC TCAAGGGGGA GAAGATCTTC TATCTCATCC GGCCGGCCTC AGCCAACCTC 
TCCCTATATG AATGCTGGCG GTCAGCCTCC AACCACAGCG AGATGTTCTT TGCTGACCAG 
GTGGACAAGT GCTATAAGTG CACTGTCAAG CAGGGCCAGA CCCTCTTCAT CCCCTCAGGC 
TGGATATACG CTACGCTCAC CCCTGTGGAC TGCCTGGCCT TCGCTGGACA TTTTCTTCAC 
AGCCTCAGTG TGGAGATGCA GATGAGGGCA TACGAGGTGG AAAGAAGGTT AAAACTAGGC 
AGCCTGACTC AATTTCCCAA CTTTGAAACT GCCTGCTGGT ACATGGGGAA GCATCTATTG 
GAAGCATTCA AAGGTTCTCA CAAGTCTGGG AAACAGCTGC CCCCACACTT AGTTCAAGGA 
GCTAAGATTC TCAATGGTGC TTTTAGGTCA TGGACGAAGA AGCAGGCTTT GGCAGAGCAC 
GAGGACGAGC TCCCGGAGCA CTTCAGGCCC TCACAGCTCA TCAAGGACCT GGCCAAAGAG 
ATCAGGCTCA GCGAGAATGC CTCCAAAACT GTCCGACCTG AAGTAAATGC TGTCGCCTCC 
TCAGATGAGG TCTGTGACGG GGACCGGGAG AAGGAGGAAC CGCCATCTCC CATTGAGTCT 
ACCCCACCTC GATCCCTCCT GGAGAAAGTG TCCAAAAAAA AGACTTCCAA GACTGTGAAG 
ATGCCCAAGC CATCCAAAAC CCCCAAGCCC CCCAAGCCCC CCAAGCCCCC CAAAACCCTG 
AAACTCAAGG ACGGAAGCAA GAAGAAAGGG AAGAAGTGCA AAGAGTCCGC CTCTCCCACC 
ATCCCCAACT TGGACCTGCT TGAGGCCCAC ACCAAGGAGG CGCTGACCAA GATGGAGCCA 
CCGAAGAAAG GAAAGACTCC AAAGAGCGTC CTGAGTGTTC CCAATAAGGA CACGGTCCAC 
ACGCAGAATG ACGTGGAAAG ACTGGAAATC CGAGAGCAAA CAAAGAGCAA GTCGGAGGCC 
AAGTGGAAGT ACAAGAACAG CAAACCTGAC TCTTTACTGA GAATGGAGGA GGAGCAGAGG 
TTGGAGAAGT CACCCCTGGC TGGGAACAAG GACAAGTTTT CCTTTTCTTT CTCTAACAGA 
AAACTCCTGG GCTCCAAGCC CCTCAGGCCG CCGAGCAGCC CTGGCGTGTT CGGGACCTTG 
CAGAGCTTCA AGGAGGACAA GGCCAAGCCC GTCCGAGATG AGTATGAATA TGTATCGGAC 
GACGGGGAGC TGAAGATTGA CGAGTTTCCT ATCAGGAGGA AGAAGAACCC CCCCAAAAGG 
GATTTGTCCT TTTTGTTAGA CAAGAAGGAG ACTCTCCTCA TCCCCACCTC GAAGCCAAAG 
CTGGACTCCA CGGCATACAA GAGCGACGAC TCCTCTGATG AGGGCTCTCT GCATATCGAC 
ACGGACACCA AGCCAAGCCG AAATGCCAAA GTGAAGAAGG AAAGTGGGAG CTCGGCGGCA 
GGCATCCTGG ACCTACTGCA GGCCAGCGAG GAGGTCGGGG CTCTTGAGTA CAACCCCAAC 
AGCCAGCCCC CTGCCTCCCC CAGTACGCAG GAAGCCATTC AGGGAATGTT GTCCATGGCC 
AATCTGCAGG CCTCTGACTC CTGCCTGCAG ACCACATGGG GCACTGGCCA GGCCAAGGGG 
GGTTCCCTGG CAGCCCATGG CGCCCGGAAG AATGGTGGTA GCAGCAAAGG GACAGGCAAG 
CGCCTACTGA AGAGGACTGC CAAGAACAGC GTGGATCTTG AGGACTATGA GGAGCAGGAC 
CACCTGGACG CCTGCTTCAA GGACTCAGAC TATGTTTACC CCTCACTGGA GTCTGACGAA 
GATAACCCTG TCTTCAAGTC CCGGTCAAAG AAGAGGAAAG GCTCAGACGA TGCTCCTTAC 
AGCCCCACAG CCAGGGTCGG TCCATCAGTG CCGAGACAAG ACAGGCCTGT GCGTGAGGGG 
ACCAGAGTGG CCTCCATTGA GACTGGGCTG GCAGCTGCTG CAGCCAAGCT GTCCCAGCAG 
GAGGAGCAGA AAAACAGGAA GAAGAAGAAC ACCAAAAGGA AGCCGACTCC TAACACTGCC 
TCCCCCTCCG TCTCCACGTC TGCCTCCGCC TCCACGGGTA CCACCTCGGC CTCCACCACC 
CCAGCCTCCA CCACCCCGGC CTCCACCACC CCAGCATCCA CCACCCCGGC CTCTACCAGC 
ACAGCCAGCA GCCAGGCCTC ACAGGAGGGC AGCTCACCCG AGCCCCCACC TGAGTCACAC 
AGCAGTGGCC TAGCTGACCA CGAGTATACA GCAGCCGGCA CATTCGCAGG GGCCCAGGCT 
GGCCGTGCCT CCCAGCCCAT GGCCCCTGGA GTCTTTCTCA CACAGAGGCG GCCTTCTGCA 
TCATCCCCCA ACAACACTGC TGCCAAAGGA AAACGTACAA AAAAGGGCAT GGCCACCGCC 
AAGCAAAGGC TTGGAAAGAT CTTGAAGATC CATCGGAATG GGAAACTGCT CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253791.1
CDS	2055..5111
Translation	MKPVQNGSQLFIKELRSRTFPSAEDVVSRVPGSQLTVGYMEEHGFTEPILVPKKDGLGLAVPAPTFYVSDVENYVGPERSVDVTDVTKQKDCKMKLKEFVDYYYSTNRKRVLNVTNLEFSDTRMSSFVEPPDIVKKLSWVENYWPDDALLAKPKVTKYCLICVKDSYTDFHIDSGGASAWYHVLKGEKIFYLIRPASANLSLYECWRSASNHSEMFFADQVDKCYKCTVKQGQTLFIPSGWIYATLTPVDCLAFAGHFLHSLSVEMQMRAYEVERRLKLGSLTQFPNFETACWYMGKHLLEAFKGSHKSGKQLPPHLVQGAKILNGAFRSWTKKQALAEHEDELPEHFRPSQLIKDLAKEIRLSENASKTVRPEVNAVASSDEVCDGDREKEEPPSPIESTPPRSLLEKVSKKKTSKTVKMPKPSKTPKPPKPPKPPKTLKLKDGSKKKGKKCKESASPTIPNLDLLEAHTKEALTKMEPPKKGKTPKSVLSVPNKDTVHTQNDVERLEIREQTKSKSEAKWKYKNSKPDSLLRMEEEQRLEKSPLAGNKDKFSFSFSNRKLLGSKPLRPPSSPGVFGTLQSFKEDKAKPVRDEYEYVSDDGELKIDEFPIRRKKNPPKRDLSFLLDKKETLLIPTSKPKLDSTAYKSDDSSDEGSLHIDTDTKPSRNAKVKKESGSSAAGILDLLQASEEVGALEYNPNSQPPASPSTQEAIQGMLSMANLQASDSCLQTTWGTGQAKGGSLAAHGARKNGGSSKGTGKRLLKRTAKNSVDLEDYEEQDHLDACFKDSDYVYPSLESDEDNPVFKSRSKKRKGSDDAPYSPTARVGPSVPRQDRPVREGTRVASIETGLAAAAAKLSQQEEQKNRKKKNTKRKPTPNTASPSVSTSASASTGTTSASTTPASTTPASTTPASTTPASTSTASSQASQEGSSPEPPPESHSSGLADHEYTAAGTFAGAQAGRASQPMAPGVFLTQRRPSASSPNNTAAKGKRTKKGMATAKQRLGKILKIHRNGKLLL

Target ORF information:

RefSeq Version	XM_006253729.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 2 (Phf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253729.2

ORF Insert Sequence:

ATGAAGCCAG TGCAGAATGG CAGCCAGCTG TTCATCAAGG AGCTGCGGAG CCGAACCTTC 
CCCAGTGCTG AAGATGTGGT GTCCCGTGTA CCAGGCAGCC AGCTCACTGT GGGCTACATG 
GAGGAGCATG GCTTCACCGA GCCCATCCTT GTCCCCAAGA AAGATGGCCT GGGCTTGGCT 
GTCCCTGCCC CAACATTCTA CGTGAGTGAC GTGGAGAACT ATGTGGGGCC AGAGAGGAGT 
GTGGACGTGA CAGACGTTAC CAAGCAGAAA GACTGCAAAA TGAAGCTGAA AGAATTCGTG 
GACTATTACT ACAGCACCAA CCGCAAACGT GTTCTCAATG TGACCAACCT CGAGTTCTCA 
GACACCAGAA TGTCCAGCTT TGTGGAGCCA CCTGACATTG TGAAGAAACT ATCGTGGGTG 
GAAAACTACT GGCCCGATGA CGCGCTGCTG GCCAAGCCCA AGGTGACCAA GTACTGCCTG 
ATCTGTGTGA AGGACAGCTA CACCGACTTC CACATTGATT CTGGAGGTGC CTCAGCCTGG 
TACCACGTTC TCAAGGGGGA GAAGATCTTC TATCTCATCC GGCCGGCCTC AGCCAACCTC 
TCCCTATATG AATGCTGGCG GTCAGCCTCC AACCACAGCG AGATGTTCTT TGCTGACCAG 
GTGGACAAGT GCTATAAGTG CACTGTCAAG CAGGGCCAGA CCCTCTTCAT CCCCTCAGGC 
TGGATATACG CTACGCTCAC CCCTGTGGAC TGCCTGGCCT TCGCTGGACA TTTTCTTCAC 
AGCCTCAGTG TGGAGATGCA GATGAGGGCA TACGAGGTGG AAAGAAGGTT AAAACTAGGC 
AGCCTGACTC AATTTCCCAA CTTTGAAACT GCCTGCTGGT ACATGGGGAA GCATCTATTG 
GAAGCATTCA AAGGTTCTCA CAAGTCTGGG AAACAGCTGC CCCCACACTT AGTTCAAGGA 
GCTAAGATTC TCAATGGTGC TTTTAGGTCA TGGACGAAGA AGCAGGCTTT GGCAGAGCAC 
GAGGACGAGC TCCCGGAGCA CTTCAGGCCC TCACAGCTCA TCAAGGACCT GGCCAAAGAG 
ATCAGGCTCA GCGAGAATGC CTCCAAAACT GTCCGACCTG AAGTAAATGC TGTCGCCTCC 
TCAGATGAGG TCTGTGACGG GGACCGGGAG AAGGAGGAAC CGCCATCTCC CATTGAGTCT 
ACCCCACCTC GATCCCTCCT GGAGAAAGTG TCCAAAAAAA AGACTTCCAA GACTGTGAAG 
ATGCCCAAGC CATCCAAAAC CCCCAAGCCC CCCAAGCCCC CCAAGCCCCC CAAAACCCTG 
AAACTCAAGG ACGGAAGCAA GAAGAAAGGG AAGAAGTGCA AAGAGTCCGC CTCTCCCACC 
ATCCCCAACT TGGACCTGCT TGAGGCCCAC ACCAAGGAGG CGCTGACCAA GATGGAGCCA 
CCGAAGAAAG GAAAGACTCC AAAGAGCGTC CTGAGTGTTC CCAATAAGGA CACGGTCCAC 
ACGCAGAATG ACGTGGAAAG ACTGGAAATC CGAGAGCAAA CAAAGAGCAA GTCGGAGGCC 
AAGTGGAAGT ACAAGAACAG CAAACCTGAC TCTTTACTGA GAATGGAGGA GGAGCAGAGG 
TTGGAGAAGT CACCCCTGGC TGGGAACAAG GACAAGTTTT CCTTTTCTTT CTCTAACAGA 
AAACTCCTGG GCTCCAAGCC CCTCAGGCCG CCGAGCAGCC CTGGCGTGTT CGGGACCTTG 
CAGAGCTTCA AGGAGGACAA GGCCAAGCCC GTCCGAGATG AGTATGAATA TGTATCGGAC 
GACGGGGAGC TGAAGATTGA CGAGTTTCCT ATCAGGAGGA AGAAGAACCC CCCCAAAAGG 
GATTTGTCCT TTTTGTTAGA CAAGAAGGAG ACTCTCCTCA TCCCCACCTC GAAGCCAAAG 
CTGGACTCCA CGGCATACAA GAGCGACGAC TCCTCTGATG AGGGCTCTCT GCATATCGAC 
ACGGACACCA AGCCAAGCCG AAATGCCAAA GTGAAGAAGG AAAGTGGGAG CTCGGCGGCA 
GGCATCCTGG ACCTACTGCA GGCCAGCGAG GAGGTCGGGG CTCTTGAGTA CAACCCCAAC 
AGCCAGCCCC CTGCCTCCCC CAGTACGCAG GAAGCCATTC AGGGAATGTT GTCCATGGCC 
AATCTGCAGG CCTCTGACTC CTGCCTGCAG ACCACATGGG GCACTGGCCA GGCCAAGGGG 
GGTTCCCTGG CAGCCCATGG CGCCCGGAAG AATGGTGGTA GCAGCAAAGG GACAGGCAAG 
CGCCTACTGA AGAGGACTGC CAAGAACAGC GTGGATCTTG AGGACTATGA GGAGCAGGAC 
CACCTGGACG CCTGCTTCAA GGACTCAGAC TATGTTTACC CCTCACTGGA GTCTGACGAA 
GATAACCCTG TCTTCAAGTC CCGGTCAAAG AAGAGGAAAG GCTCAGACGA TGCTCCTTAC 
AGCCCCACAG CCAGGGTCGG TCCATCAGTG CCGAGACAAG ACAGGCCTGT GCGTGAGGGG 
ACCAGAGTGG CCTCCATTGA GACTGGGCTG GCAGCTGCTG CAGCCAAGCT GTCCCAGCAG 
GAGGAGCAGA AAAACAGGAA GAAGAAGAAC ACCAAAAGGA AGCCGACTCC TAACACTGCC 
TCCCCCTCCG TCTCCACGTC TGCCTCCGCC TCCACGGGTA CCACCTCGGC CTCCACCACC 
CCAGCCTCCA CCACCCCGGC CTCCACCACC CCAGCATCCA CCACCCCGGC CTCTACCAGC 
ACAGCCAGCA GCCAGGCCTC ACAGGAGGGC AGCTCACCCG AGCCCCCACC TGAGTCACAC 
AGCAGTGGCC TAGCTGACCA CGAGTATACA GCAGCCGGCA CATTCGCAGG GGCCCAGGCT 
GGCCGTGCCT CCCAGCCCAT GGCCCCTGGA GTCTTTCTCA CACAGAGGCG GCCTTCTGCA 
TCATCCCCCA ACAACACTGC TGCCAAAGGA AAACGTACAA AAAAGGGCAT GGCCACCGCC 
AAGCAAAGGC TTGGAAAGAT CTTGAAGATC CATCGGAATG GGAAACTGCT CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone lysine methylation dynamics: establishment, regulation, and biological impact.
Molecular cell48(4)491-507(2012 Nov)
Black JC,Van Rechem C,Whetstine JR

PKA-dependent regulation of the histone lysine demethylase complex PHF2-ARID5B.
Nature cell biology13(6)668-75(2011 Jun)
Baba A,Ohtake F,Okuno Y,Yokota K,Okada M,Imai Y,Ni M,Meyer CA,Igarashi K,Kanno J,Brown M,Kato S

Structural basis for human PHF2 Jumonji domain interaction with metal ions.
Journal of molecular biology406(1)1-8(2011 Feb)
Horton JR,Upadhyay AK,Hashimoto H,Zhang X,Cheng X

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Cell142(5)810-21(2010 Sep)
Ohta S,Bukowski-Wills JC,Sanchez-Pulido L,Alves Fde L,Wood L,Chen ZA,Platani M,Fischer L,Hudson DF,Ponting CP,Fukagawa T,Earnshaw WC,Rappsilber J

Recognition of histone H3K4 trimethylation by the plant homeodomain of PHF2 modulates histone demethylation.
The Journal of biological chemistry285(13)9322-6(2010 Mar)
Wen H,Li J,Song T,Lu M,Kan PY,Lee MG,Sha B,Shi X