Lrrc8e cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrrc8e
Entrez Gene ID	304203
Full Name	leucine rich repeat containing 8 VRAC subunit E
Synonyms	RGD1311979
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit E Names volume-regulated anion channel subunit LRRC8E leucine rich repeat containing 8 family, member E leucine-rich repeat-containing protein 8E
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p12

mRNA and Protein(s)

mRNA	Protein	Name
XM_008768954.1	XP_008767176.1	volume-regulated anion channel subunit LRRC8E isoform X1
XM_006248764.3	XP_006248826.1	volume-regulated anion channel subunit LRRC8E isoform X1
XM_006248763.3	XP_006248825.1	volume-regulated anion channel subunit LRRC8E isoform X1
NM_001034139.1	NP_001029311.1	volume-regulated anion channel subunit LRRC8E

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8E	NP_001255213.1
Pan troglodytes (chimpanzee)	LRRC8E	XP_003953389.1
Canis lupus familiaris (dog)	LRRC8E	XP_005633001.1
Bos taurus (cattle)	LRRC8E	NP_001192551.1
Rattus norvegicus (Norway rat)	Lrrc8e	NP_001029311.1
Xenopus tropicalis (tropical clawed frog)	lrrc8e	NP_001007863.1
	LRRC8E	XP_001093244.1
Mus musculus (house mouse)	Lrrc8e	NP_082451.2

Gene Ontology
Bibliography

Related articles in PubMed

Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels.
Lutter D, Ullrich F, Lueck JC, Kempa S, Jentsch TJ
Journal of cell science130(6)1122-1133(2017 03)

LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength.
Syeda R, Qiu Z, Dubin AE, Murthy SE, Florendo MN, Mason DE, Mathur J, Cahalan SM, Peters EC, Montal M, Patapoutian A
Cell164(3)499-511(2016 Jan)

Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC.
Voss FK, Ullrich F, M?nch J, Lazarow K, Lutter D, Mah N, Andrade-Navarro MA, von Kries JP, Stauber T, Jentsch TJ
Science (New York, N.Y.)344(6184)634-8(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lrrc8e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrrc8e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08888	NM_001034139.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 VRAC subunit E (Lrrc8e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa34537	XM_006248763.3 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa34537	XM_008768954.1 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa34537	XM_006248764.3 Latest version!	Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrrc8e ( NM_001034139.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029311.1 Rattus norvegicus leucine rich repeat containing 8 VRAC subunit E (Lrrc8e), mRNA.

Price & Availability↑

CloneID	ORa08888
Clone ID Related Accession (Same CDS sequence)	NM_001034139.1
Accession Version	NM_001034139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105779.1. On Oct 14, 2005 this sequence version replaced XM_221814.2. ##Evidence-Data-START## Transcript exon combination :: BC105779.1, DN948377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632254, SAMN00632258 [ECO:0000348] ##Evidence-Data-END##

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTCCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029311.1
CDS	73..2460
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHESRENSSEAPCQQLLPQGISEQIGGLRELSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRATVTTVTTVGTGTGKVGEGEKEKVLIEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYSMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEITGYASFCCNHTKAHLFSKLAFCYISFVCVYGITCLYTLYWLFHRPLKEYSFRSVREETGMNDIPDVRNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPDKLRQKLQRNARGRLELSLCMLPGLPDTVFELSEVEALRLEAICDISFPPGLSQLVNLQELSLLHSPARLPFSSQIFLRDRLKVICVKFEELREVPLWVFGLRGLEELHLEGLFPPEMARGATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAVLRELELVACGLERIPHAIFSLGALQELDLKDNHLRSIEEILSFQHCRKLVILRLWHNQIAYVPEHVRKLRSLEQLYLSHNKLETLPAQLGQCFGLRLLDVSHNGLRSLPPELGLLQSLQHLALSYNALESLPDELFFCHKLRTLLLGYNHLTQFSPHVAALQALSRLELKGNRLEALPEELGDCKGLKKSGLLVEDTLYEGLPAEVREKMEEE

Target ORF information:

RefSeq Version	NM_001034139.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 VRAC subunit E (Lrrc8e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034139.1

ORF Insert Sequence:

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTCCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8e ( XM_008768954.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767176.1 Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa34537
Clone ID Related Accession (Same CDS sequence)	XM_008768954.1 , XM_006248764.3 , XM_006248763.3
Accession Version	XM_008768954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTTCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767176.1
CDS	377..2764
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHESRENSSEAPCQQLLPQGISEQIGGLRELSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRATVTTVTTVGTGTGKVGEGEKEKVLIEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYSMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEITGYASFCCNHTKAHLFSKLAFCYISFVCVYGITCLYTLYWLFHRPLKEYSFRSVREETGMNDIPDVRNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPDKLRQKLQRNARGRLELSLCMLPGLPDTVFELSEVEALRLEAICDISFPPGLSQLVNLQELSLLHSPARLPFSSQIFLRDRLKVICVKFEELREVPLWVFGLRGLEELHLEGLFPPEMARGATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAVLRELELVACGLERIPHAIFSLGALQELDLKDNHLRSIEEILSFQHCRKLVILRLWHNQIAYVPEHVRKLRSLEQLYLSHNKLETLPAQLGQCFGLRLLDVSHNGLRSLPPELGLLQSLQHLALSYNALESLPDELFFCHKLRTLLLGYNHLTQFSPHVAALQALSRLELKGNRLEALPEELGDCKGLKKSGLLVEDTLYEGLPAEVREKMEEE

Target ORF information:

RefSeq Version	XM_008768954.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768954.1

ORF Insert Sequence:

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTTCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8e ( XM_006248764.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248826.1 Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa34537
Clone ID Related Accession (Same CDS sequence)	XM_008768954.1 , XM_006248764.3 , XM_006248763.3
Accession Version	XM_006248764.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTTCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248826.1
CDS	244..2631
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHESRENSSEAPCQQLLPQGISEQIGGLRELSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRATVTTVTTVGTGTGKVGEGEKEKVLIEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYSMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEITGYASFCCNHTKAHLFSKLAFCYISFVCVYGITCLYTLYWLFHRPLKEYSFRSVREETGMNDIPDVRNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPDKLRQKLQRNARGRLELSLCMLPGLPDTVFELSEVEALRLEAICDISFPPGLSQLVNLQELSLLHSPARLPFSSQIFLRDRLKVICVKFEELREVPLWVFGLRGLEELHLEGLFPPEMARGATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAVLRELELVACGLERIPHAIFSLGALQELDLKDNHLRSIEEILSFQHCRKLVILRLWHNQIAYVPEHVRKLRSLEQLYLSHNKLETLPAQLGQCFGLRLLDVSHNGLRSLPPELGLLQSLQHLALSYNALESLPDELFFCHKLRTLLLGYNHLTQFSPHVAALQALSRLELKGNRLEALPEELGDCKGLKKSGLLVEDTLYEGLPAEVREKMEEE

Target ORF information:

RefSeq Version	XM_006248764.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248764.3

ORF Insert Sequence:

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTTCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrc8e ( XM_006248763.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006248825.1 Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34537
Clone ID Related Accession (Same CDS sequence)	XM_008768954.1 , XM_006248764.3 , XM_006248763.3
Accession Version	XM_006248763.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	volume-regulated anion channel subunit LRRC8E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006248763.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTTCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006248825.1
CDS	111..2498
Translation	MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPSHESRENSSEAPCQQLLPQGISEQIGGLRELSGLKNNLDLQQYSFINQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENHKGPAAGRATVTTVTTVGTGTGKVGEGEKEKVLIEPEKVVTEPPAVTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYSMYIRQTVLKVCKFFAILVYNLVYVEKISFLVACRVETSEITGYASFCCNHTKAHLFSKLAFCYISFVCVYGITCLYTLYWLFHRPLKEYSFRSVREETGMNDIPDVRNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPDKLRQKLQRNARGRLELSLCMLPGLPDTVFELSEVEALRLEAICDISFPPGLSQLVNLQELSLLHSPARLPFSSQIFLRDRLKVICVKFEELREVPLWVFGLRGLEELHLEGLFPPEMARGATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLLALNSLKKLAVLRELELVACGLERIPHAIFSLGALQELDLKDNHLRSIEEILSFQHCRKLVILRLWHNQIAYVPEHVRKLRSLEQLYLSHNKLETLPAQLGQCFGLRLLDVSHNGLRSLPPELGLLQSLQHLALSYNALESLPDELFFCHKLRTLLLGYNHLTQFSPHVAALQALSRLELKGNRLEALPEELGDCKGLKKSGLLVEDTLYEGLPAEVREKMEEE

Target ORF information:

RefSeq Version	XM_006248763.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing 8 family, member E (Lrrc8e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006248763.3

ORF Insert Sequence:

ATGATCCCAG TGGCGGAGTT CAAGCAGTTC ACGGAGCAGC AGCCTGCGTT CAAGGTGCTC 
AAACCCTGGT GGGATGTTTT GGCTGAGTAC CTCACTGTGG CTATGCTCAT GATTGGGGTG 
TTCGGGTGCA CCCTCCAGGT GACACAGGAC AAGATCATCT GCCTGCCCAG CCATGAATCT 
CGTGAGAACT CATCAGAGGC TCCTTGCCAG CAGCTGTTGC CTCAAGGGAT CTCTGAGCAG 
ATTGGAGGCC TACGAGAGCT CAGCGGCCTC AAGAATAACC TAGACCTCCA GCAATACAGC 
TTTATTAACC AGCTCTGCTA TGAGACTGCC CTCCACTGGT ACGCCAAGTA CTTCCCCTAC 
CTGGTGGTCA TTCATACTCT TATCTTTATG GTCTGTACCA GCTTTTGGTT CAAATTCCCT 
GGTACCAGCT CCAAAATAGA ACACTTCATC TCTATCCTGG GCAAGTGTTT CGACTCTCCG 
TGGACCACTC GGGCCTTGTC TGAGGTGTCT GGAGAAAATC ACAAGGGCCC AGCTGCTGGA 
CGGGCCACGG TGACCACAGT AACCACGGTG GGGACAGGGA CAGGGAAGGT GGGCGAGGGT 
GAGAAGGAGA AAGTGCTGAT AGAGCCTGAG AAGGTGGTGA CTGAACCCCC AGCTGTAACC 
CTGCTGGATA AGAAGGAGGG CGAACAAGCT AAGGCCCTAT TTGAGAAGGT CAAAAAGTTC 
CGAGTGCACG TGGAAGAGGG TGACATCCTC TACTCCATGT ACATCCGGCA GACGGTGCTC 
AAAGTGTGTA AGTTTTTTGC CATCCTGGTT TACAACCTGG TCTATGTGGA GAAGATCAGC 
TTTCTGGTGG CCTGTAGGGT GGAGACATCA GAGATCACAG GCTACGCCAG TTTCTGCTGC 
AACCACACCA AGGCCCACCT CTTTTCTAAG CTGGCCTTCT GCTACATCTC CTTCGTGTGT 
GTCTATGGCA TCACCTGCCT GTACACGCTC TACTGGCTCT TCCACAGGCC CCTTAAAGAG 
TACTCCTTCC GTTCTGTGCG GGAGGAGACA GGGATGAATG ATATCCCGGA TGTTAGGAAT 
GACTTCGCCT TCATGCTCCA CCTCATTGAC CAGTATGACT CCCTCTATTC TAAACGGTTT 
GCAGTCTTCC TCTCTGAAGT CAGTGAAAGC CGCCTTAAGC AGCTCAACCT CAACCACGAG 
TGGACACCTG ATAAGCTGAG GCAGAAGCTG CAGCGTAACG CACGCGGTCG GCTGGAGCTG 
AGCCTCTGTA TGCTCCCTGG GCTTCCGGAC ACTGTCTTTG AGCTCAGTGA GGTAGAGGCG 
CTCAGACTGG AGGCCATCTG CGACATCTCC TTTCCACCAG GGCTATCCCA GCTCGTGAAC 
CTTCAGGAGC TCAGCCTGCT TCACTCACCA GCCAGGCTGC CCTTCTCTTC ACAGATCTTC 
CTGCGAGACC GGCTGAAAGT GATCTGTGTC AAGTTTGAGG AGCTCCGCGA GGTACCCCTC 
TGGGTGTTTG GGCTCCGAGG CTTGGAAGAA CTACACCTGG AAGGACTCTT CCCCCCAGAA 
ATGGCTCGGG GTGCCACCCT CGAGAGCCTC CGGGAGCTTA AACAGCTCAA GGTACTGTCT 
CTTCGTAGCA ATGCAGGGAA GGTGCCAGCC AGCGTGACCG ATGTAGCCGG GCACCTTCAG 
CGACTCAGCC TGCACAATGA TGGCGCTCGT CTGCTCGCCT TAAACAGCCT CAAGAAGCTG 
GCGGTGTTAC GGGAGCTGGA GCTGGTGGCC TGTGGGCTGG AACGTATCCC CCATGCAATC 
TTCAGCCTGG GGGCTCTGCA GGAACTCGAC CTTAAGGACA ACCACCTCCG GTCTATTGAG 
GAGATCCTCA GCTTCCAGCA CTGTCGGAAG CTGGTCATTC TCAGGCTGTG GCACAACCAG 
ATAGCCTATG TGCCTGAGCA TGTGAGGAAG CTCCGAAGCC TGGAGCAGCT CTACCTCAGT 
CACAACAAGC TTGAGACTCT GCCTGCCCAG CTTGGCCAGT GCTTTGGCCT CCGCCTGCTG 
GATGTGTCCC ACAACGGGCT TCGTTCCCTG CCACCTGAGT TGGGTCTTCT CCAGAGTCTC 
CAGCATCTAG CCCTCTCCTA CAACGCACTT GAGAGCCTGC CTGATGAACT GTTCTTCTGC 
CATAAGCTGC GGACATTACT CTTGGGCTAC AACCACCTGA CTCAGTTTTC GCCCCACGTG 
GCTGCCCTTC AGGCCCTCAG CCGTCTGGAG CTCAAGGGTA ACAGGTTGGA GGCACTGCCA 
GAAGAACTCG GTGACTGCAA GGGGCTGAAA AAGTCAGGGC TCCTGGTGGA GGACACCCTT 
TATGAGGGGC TGCCTGCAGA GGTTCGGGAG AAAATGGAAG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels.
Journal of cell science130(6)1122-1133(2017 03)
Lutter D,Ullrich F,Lueck JC,Kempa S,Jentsch TJ

LRRC8 Proteins Form Volume-Regulated Anion Channels that Sense Ionic Strength.
Cell164(3)499-511(2016 Jan)
Syeda R,Qiu Z,Dubin AE,Murthy SE,Florendo MN,Mason DE,Mathur J,Cahalan SM,Peters EC,Montal M,Patapoutian A

Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC.
Science (New York, N.Y.)344(6184)634-8(2014 May)
Voss FK,Ullrich F,M?nch J,Lazarow K,Lutter D,Mah N,Andrade-Navarro MA,von Kries JP,Stauber T,Jentsch TJ

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Lrrc8e cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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