Lgr5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lgr5
Entrez Gene ID	299802
Full Name	leucine rich repeat containing G protein coupled receptor 5
Synonyms	Gpr49
General protein information	Preferred Names leucine rich repeat containing G protein coupled receptor 5 Names leucine-rich repeat-containing G-protein coupled receptor 5 G protein-coupled receptor 49
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594740.1	XP_017450229.1	leucine-rich repeat-containing G-protein coupled receptor 5 isoform X1
NM_001106784.1	NP_001100254.1	leucine-rich repeat-containing G-protein coupled receptor 5 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LGR5	XP_003313909.1
	LGR5	XP_001117502.1
Drosophila melanogaster (fruit fly)	rk	NP_476702.1
Mus musculus (house mouse)	Lgr5	NP_034325.2
Homo sapiens (human)	LGR5	NP_003658.1
Gallus gallus (chicken)	LGR5	XP_425441.2
Rattus norvegicus (Norway rat)	Lgr5	NP_001100254.1
Bos taurus (cattle)	LGR5	NP_001179449.1
Anopheles gambiae (African malaria mosquito)	GPRRK	XP_317111.1
Xenopus tropicalis (tropical clawed frog)	lgr5	XP_002940219.2

Gene Ontology
Bibliography

Related articles in PubMed

Influence of stress factors on intestinal epithelial injury and regeneration.
Lee C, Minich A, Li B, Miyake H, Seo S, Pierro A
Pediatric surgery international(2017 Oct)

Temporal and Spatial Expression of LGR5 After Acute Spinal Cord Injury in Adult Rats.
Chen X, Hao J, Fu T, Liu J, Yu M, He S, Qian R, Zhang F
Neurochemical research41(10)2645-2654(2016 Oct)

Targeting LGR5+ cells with an antibody-drug conjugate for the treatment of colon cancer.
Junttila MR, Mao W, Wang X, Wang BE, Pham T, Flygare J, Yu SF, Yee S, Goldenberg D, Fields C, Eastham-Anderson J, Singh M, Vij R, Hongo JA, Firestein R, Schutten M, Flagella K, Polakis P, Polson AG
Science translational medicine7(314)314ra186(2015 Nov)

Notch inhibition induces mitotically generated hair cells in mammalian cochleae via activating the Wnt pathway.
Li W, Wu J, Yang J, Sun S, Chai R, Chen ZY, Li H
Proceedings of the National Academy of Sciences of the United States of America112(1)166-71(2015 Jan)

Marker genes identify three somatic cell types in the fetal mouse ovary.
Rastetter RH, Bernard P, Palmer JS, Chassot AA, Chen H, Western PS, Ramsay RG, Chaboissier MC, Wilhelm D
Developmental biology394(2)242-52(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

that Leucine-rich repeat-containing G-protein coupled receptor 5might be of great significance in CNS pathophysiology after spinal cord injury
Title: Temporal and Spatial Expression of LGR5 After Acute Spinal Cord Injury in Adult Rats.

Report targeting LGR5+ tumor stem cells with an antibody-drug conjugate as effective treatment for colon cancer.
Title: Targeting LGR5+ cells with an antibody-drug conjugate for the treatment of colon cancer.

Results support LGR-5 as a putative neoplastic stem cell marker in colonic tumors co-expressing nuclear beta-catenin.
Title: Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early phases of 1,2-dimethylhydrazine-induced rat colon carcinogenesis: correlation with nuclear ?-catenin.

The following Lgr5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lgr5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02206	NM_001106784.1 Latest version!	Rattus norvegicus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa61942	XM_017594740.1 Latest version!	Rattus norvegicus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lgr5 ( NM_001106784.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100254.1 Rattus norvegicus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), mRNA.

Price & Availability↑

CloneID	ORa02206
Clone ID Related Accession (Same CDS sequence)	NM_001106784.1
Accession Version	NM_001106784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473960.2. On Oct 4, 2007 this sequence version replaced XM_235149.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACACCT CCCGCGTCCG CATGCTCCTG TCCTTGCTGG CGCTGCTGCA GCTGGTGGCT 
GCCGGCAGCC CACCGAGACC GGACACGATG CCGCGGGGCT GCCCATCATA CTGTCACTGT 
GAGCTGGATG GCAGGATGCT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCCTCCAACC TCAGCGTCTT CACCTCCTAC CTGGACCTCA GTATGAACAA CATCAGTCAG 
CTACCTGCCA GTCTCCTGCA TCGCCTCCGC TTCCTAGAAG AGTTGCGTCT TGCTGGAAAT 
GCTTTGACAC ACATTCCCAA GGGAGCATTC GCAGGCCTTC ACAGTCTCAA AGTTCTCATG 
CTGCAGAATA ACCAGCTGAG ACAGGTTCCG GAGGAAGCGC TACAGAACCT GAGAAGCCTT 
CAGTCCCTGC GCTTGGATGC CAACCACATC AGCTACGTGC CACCCAGCTG TTTCAGTGGT 
CTGCACTCCC TGAGGCACCT GTGGCTAGAT GACAACGCTC TCACAGATGT CCCTGTCCAG 
GCTTTCAGAA GTTTATCAGC CCTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATAGCAGACC ATGCCTTTGG AAACCTCTCC AGCCTCGTGG TTCTGCATCT CCATAATAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGACTCC ACAGCCTGGA GACTTTAGAT 
TTAAATTATA ATAACCTTGA CGAATTCCCC ACTGCAATCA AGACACTCTC CAACCTCAAG 
GAACTAGGAT TCCACAGCAA CAATATCAGA TCAATACCAG AGCGAGCATT CGTAGGCAAC 
CCTTCTCTTA TCACAATACA TTTCTATGAC AATCCCATCC AGTTTGTTGG AATCTCTGCT 
TTTCAACACT TGCCTGAACT AAGAACGCTG ACTTTGAATG GTGCCTCGCA AATTACTGAA 
TTCCCTGACT TAACGGGGAC CGCAACCCTG GAGAGCCTGA CTTTAACTGG AGCAAAGATC 
TCGTCTCTTC CCCAAACCGT CTGTGATCAG TTACCTAATC TCCAAGTGCT AGATTTGTCT 
TACAACCTGC TCGAAGACTT ACCCAGTTTG TCGGGCTGCC AAAAGCTTCA GAAAATTGAC 
CTGCGGCATA ATGAAATCTA TGAAATTAAA GGTGGCACTT TTCAGCAGTT GTTCAACCTC 
CGATCTCTGA ACTTAGCCCG GAATAAAATT GCCATTATTC ACCCCAACGC TTTCTCTACG 
TTGCCGTCTC TAATAAAGCT GGACCTATCA TCCAACCTCC TTTCGTCTTT CCCTGTGACT 
GGGTTACACG GTTTAACTCA CTTAAAATTA ACGGGGAACC GCGCCTTGCA GAGCCTGATA 
CCATCTGCAA ACTTCCCGGA GCTCAAGATT ATAGAAATGC CATACGCTTA CCAGTGCTGT 
GCATTTGGGG GGTGTGAGAA TGTCTATAAA ATTCCTAATC AATGGAACAA AGATGACAGC 
AGCAGTGTGG ACGACCTTCG TAAGAAAGAT GCTGGCTTAT TTCAAGTTCA AGATGAGCGG 
GACCTTGAAG ACTTCCTACT TGACTTTGAG GAAGACCTGA AGGTTCTTCA CTCCGTGCAG 
TGCTCACCTC CCCCAGGTCC CTTCAAGCCC TGTGAGCACC TGTTTGGTAG CTGGCTGATC 
CGAATCGGGG TGTGGACCAC AGCAGTCCTG GCGCTTTCCT GCAATGCCTT GGTGGCTTTC 
ACAGTGTTCA GAACCCCTCT GTACATCTCT TCCATAAAAC TGCTAATCGG GGTAATTGCG 
GTAGTGGACA TACTCATGGG GGTCTCCAGT GCAATTCTGG CTGTCGTGGA CACGTTCACT 
TTTGGCAGTT TTGCTCAACA CGGTGCATGG TGGGAAGGTG GAATTGGCTG CCAAATTGTT 
GGTTTTTTGT CCATTTTTGC TTCCGAATCA TCGGTCTTCC TGCTCACTCT GGCGGCACTG 
GAACGTGGTT TTTCTGTCAA GTGCTCTTCA AAGTTTGAAA TGAAAGCTCC CCTTTCTAGC 
CTGAAAGCGA TCATTTTGCT GTGTGTCCTC TTGGCCCTGA CCATTGCCAC CGTCCCCTTG 
CTAGGAGGCA GTGAGTACAA TGCCTCTCCC CTCTGCCTGC CCTTACCCTT TGGGGAGCCC 
AGCACCACAG GCTACATGGT GGCTCTCGTA TTGCTCAACT CCCTCTGTTT CCTCATAATG 
ACCATTGCCT ATACAAGGCT CTACTGCAGT TTGGAGAAGG GAGAGCTGGA GAACCTTTGG 
GACTGTTCTA TGGTGAAGCA CACTGCTCTG TTGCTCTTCA CCAACTGTAT CCTTTACTGC 
CCCGTGGCTT TCTTATCCTT CTCCTCTTTG CTAAACCTCA CGTTTATCAG TCCCGAAGTA 
ATTAAATTTA TACTTCTAGT GATTGTCCCA CTTCCGGCAT GCCTCAACCC CCTTCTCTAC 
ATTGTCTTCA ACCCCCATTT TAAGGAGGAT ATGGGCAGCC TGGGAAAGCA AACCCGTTTC 
TGGACAAGAG CAAAACACCC AAGTCTGCTG TCCATTAACT CGGACGATGT TGAGAAACGG 
TCCTGTGACT CAACCCAAGC CTTAGTATCC TTTACCCACG CCAGCATAGC CTATGACTTG 
CCTTCCGATT CCGGGTCATC ACCAGCTTAC CCAATGACTG AAAGCTGTCA CCTCTCTTCA 
GTTGCATTTG TCCCATGTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100254.1
CDS	1..2724
Translation	MDTSRVRMLLSLLALLQLVAAGSPPRPDTMPRGCPSYCHCELDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLPASLLHRLRFLEELRLAGNALTHIPKGAFAGLHSLKVLMLQNNQLRQVPEEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTDVPVQAFRSLSALQAMTLALNKIHHIADHAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIKTLSNLKELGFHSNNIRSIPERAFVGNPSLITIHFYDNPIQFVGISAFQHLPELRTLTLNGASQITEFPDLTGTATLESLTLTGAKISSLPQTVCDQLPNLQVLDLSYNLLEDLPSLSGCQKLQKIDLRHNEIYEIKGGTFQQLFNLRSLNLARNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPVTGLHGLTHLKLTGNRALQSLIPSANFPELKIIEMPYAYQCCAFGGCENVYKIPNQWNKDDSSSVDDLRKKDAGLFQVQDERDLEDFLLDFEEDLKVLHSVQCSPPPGPFKPCEHLFGSWLIRIGVWTTAVLALSCNALVAFTVFRTPLYISSIKLLIGVIAVVDILMGVSSAILAVVDTFTFGSFAQHGAWWEGGIGCQIVGFLSIFASESSVFLLTLAALERGFSVKCSSKFEMKAPLSSLKAIILLCVLLALTIATVPLLGGSEYNASPLCLPLPFGEPSTTGYMVALVLLNSLCFLIMTIAYTRLYCSLEKGELENLWDCSMVKHTALLLFTNCILYCPVAFLSFSSLLNLTFISPEVIKFILLVIVPLPACLNPLLYIVFNPHFKEDMGSLGKQTRFWTRAKHPSLLSINSDDVEKRSCDSTQALVSFTHASIAYDLPSDSGSSPAYPMTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	NM_001106784.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106784.1

ORF Insert Sequence:

ATGGACACCT CCCGCGTCCG CATGCTCCTG TCCTTGCTGG CGCTGCTGCA GCTGGTGGCT 
GCCGGCAGCC CACCGAGACC GGACACGATG CCGCGGGGCT GCCCATCATA CTGTCACTGT 
GAGCTGGATG GCAGGATGCT GCTCAGGGTG GACTGCTCCG ACCTGGGGCT CTCGGAGCTG 
CCCTCCAACC TCAGCGTCTT CACCTCCTAC CTGGACCTCA GTATGAACAA CATCAGTCAG 
CTACCTGCCA GTCTCCTGCA TCGCCTCCGC TTCCTAGAAG AGTTGCGTCT TGCTGGAAAT 
GCTTTGACAC ACATTCCCAA GGGAGCATTC GCAGGCCTTC ACAGTCTCAA AGTTCTCATG 
CTGCAGAATA ACCAGCTGAG ACAGGTTCCG GAGGAAGCGC TACAGAACCT GAGAAGCCTT 
CAGTCCCTGC GCTTGGATGC CAACCACATC AGCTACGTGC CACCCAGCTG TTTCAGTGGT 
CTGCACTCCC TGAGGCACCT GTGGCTAGAT GACAACGCTC TCACAGATGT CCCTGTCCAG 
GCTTTCAGAA GTTTATCAGC CCTGCAAGCC ATGACCTTGG CCCTGAACAA AATACACCAC 
ATAGCAGACC ATGCCTTTGG AAACCTCTCC AGCCTCGTGG TTCTGCATCT CCATAATAAT 
AGAATCCACT CCCTGGGAAA GAAATGCTTT GATGGACTCC ACAGCCTGGA GACTTTAGAT 
TTAAATTATA ATAACCTTGA CGAATTCCCC ACTGCAATCA AGACACTCTC CAACCTCAAG 
GAACTAGGAT TCCACAGCAA CAATATCAGA TCAATACCAG AGCGAGCATT CGTAGGCAAC 
CCTTCTCTTA TCACAATACA TTTCTATGAC AATCCCATCC AGTTTGTTGG AATCTCTGCT 
TTTCAACACT TGCCTGAACT AAGAACGCTG ACTTTGAATG GTGCCTCGCA AATTACTGAA 
TTCCCTGACT TAACGGGGAC CGCAACCCTG GAGAGCCTGA CTTTAACTGG AGCAAAGATC 
TCGTCTCTTC CCCAAACCGT CTGTGATCAG TTACCTAATC TCCAAGTGCT AGATTTGTCT 
TACAACCTGC TCGAAGACTT ACCCAGTTTG TCGGGCTGCC AAAAGCTTCA GAAAATTGAC 
CTGCGGCATA ATGAAATCTA TGAAATTAAA GGTGGCACTT TTCAGCAGTT GTTCAACCTC 
CGATCTCTGA ACTTAGCCCG GAATAAAATT GCCATTATTC ACCCCAACGC TTTCTCTACG 
TTGCCGTCTC TAATAAAGCT GGACCTATCA TCCAACCTCC TTTCGTCTTT CCCTGTGACT 
GGGTTACACG GTTTAACTCA CTTAAAATTA ACGGGGAACC GCGCCTTGCA GAGCCTGATA 
CCATCTGCAA ACTTCCCGGA GCTCAAGATT ATAGAAATGC CATACGCTTA CCAGTGCTGT 
GCATTTGGGG GGTGTGAGAA TGTCTATAAA ATTCCTAATC AATGGAACAA AGATGACAGC 
AGCAGTGTGG ACGACCTTCG TAAGAAAGAT GCTGGCTTAT TTCAAGTTCA AGATGAGCGG 
GACCTTGAAG ACTTCCTACT TGACTTTGAG GAAGACCTGA AGGTTCTTCA CTCCGTGCAG 
TGCTCACCTC CCCCAGGTCC CTTCAAGCCC TGTGAGCACC TGTTTGGTAG CTGGCTGATC 
CGAATCGGGG TGTGGACCAC AGCAGTCCTG GCGCTTTCCT GCAATGCCTT GGTGGCTTTC 
ACAGTGTTCA GAACCCCTCT GTACATCTCT TCCATAAAAC TGCTAATCGG GGTAATTGCG 
GTAGTGGACA TACTCATGGG GGTCTCCAGT GCAATTCTGG CTGTCGTGGA CACGTTCACT 
TTTGGCAGTT TTGCTCAACA CGGTGCATGG TGGGAAGGTG GAATTGGCTG CCAAATTGTT 
GGTTTTTTGT CCATTTTTGC TTCCGAATCA TCGGTCTTCC TGCTCACTCT GGCGGCACTG 
GAACGTGGTT TTTCTGTCAA GTGCTCTTCA AAGTTTGAAA TGAAAGCTCC CCTTTCTAGC 
CTGAAAGCGA TCATTTTGCT GTGTGTCCTC TTGGCCCTGA CCATTGCCAC CGTCCCCTTG 
CTAGGAGGCA GTGAGTACAA TGCCTCTCCC CTCTGCCTGC CCTTACCCTT TGGGGAGCCC 
AGCACCACAG GCTACATGGT GGCTCTCGTA TTGCTCAACT CCCTCTGTTT CCTCATAATG 
ACCATTGCCT ATACAAGGCT CTACTGCAGT TTGGAGAAGG GAGAGCTGGA GAACCTTTGG 
GACTGTTCTA TGGTGAAGCA CACTGCTCTG TTGCTCTTCA CCAACTGTAT CCTTTACTGC 
CCCGTGGCTT TCTTATCCTT CTCCTCTTTG CTAAACCTCA CGTTTATCAG TCCCGAAGTA 
ATTAAATTTA TACTTCTAGT GATTGTCCCA CTTCCGGCAT GCCTCAACCC CCTTCTCTAC 
ATTGTCTTCA ACCCCCATTT TAAGGAGGAT ATGGGCAGCC TGGGAAAGCA AACCCGTTTC 
TGGACAAGAG CAAAACACCC AAGTCTGCTG TCCATTAACT CGGACGATGT TGAGAAACGG 
TCCTGTGACT CAACCCAAGC CTTAGTATCC TTTACCCACG CCAGCATAGC CTATGACTTG 
CCTTCCGATT CCGGGTCATC ACCAGCTTAC CCAATGACTG AAAGCTGTCA CCTCTCTTCA 
GTTGCATTTG TCCCATGTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lgr5 ( XM_017594740.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450229.1 Rattus norvegicus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61942
Clone ID Related Accession (Same CDS sequence)	XM_017594740.1
Accession Version	XM_017594740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	leucine-rich repeat-containing G-protein coupled receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAACA TCAGTCAGCT ACCTGCCAGT CTCCTGCATC GCCTCCGCTT CCTAGAAGAG 
TTGCGTCTTG CTGGAAATGC TTTGACACAC ATTCCCAAGG GAGCATTCGC AGGCCTTCAC 
AGTCTCAAAG TTCTCATGCT GCAGAATAAC CAGCTGAGAC AGGTTCCGGA GGAAGCGCTA 
CAGAACCTGA GAAGCCTTCA GTCCCTGCGC TTGGATGCCA ACCACATCAG CTACGTGCCA 
CCCAGCTGTT TCAGTGGTCT GCACTCCCTG AGGCACCTGT GGCTAGATGA CAACGCTCTC 
ACAGATGTCC CTGTCCAGGC TTTCAGAAGT TTATCAGCCC TGCAAGCCAT GACCTTGGCC 
CTGAACAAAA TACACCACAT AGCAGACCAT GCCTTTGGAA ACCTCTCCAG CCTCGTGGTT 
CTGCATCTCC ATAATAATAG AATCCACTCC CTGGGAAAGA AATGCTTTGA TGGACTCCAC 
AGCCTGGAGA CTTTAGATTT AAATTATAAT AACCTTGACG AATTCCCCAC TGCAATCAAG 
ACACTCTCCA ACCTCAAGGA ACTAGGATTC CACAGCAACA ATATCAGATC AATACCAGAG 
CGAGCATTCG TAGGCAACCC TTCTCTTATC ACAATACATT TCTATGACAA TCCCATCCAG 
TTTGTTGGAA TCTCTGCTTT TCAACACTTG CCTGAACTAA GAACGCTGAC TTTGAATGGT 
GCCTCGCAAA TTACTGAATT CCCTGACTTA ACGGGGACCG CAACCCTGGA GAGCCTGACT 
TTAACTGGAG CAAAGATCTC GTCTCTTCCC CAAACCGTCT GTGATCAGTT ACCTAATCTC 
CAAGTGCTAG ATTTGTCTTA CAACCTGCTC GAAGACTTAC CCAGTTTGTC GGGCTGCCAA 
AAGCTTCAGA AAATTGACCT GCGGCATAAT GAAATCTATG AAATTAAAGG TGGCACTTTT 
CAGCAGTTGT TCAACCTCCG ATCTCTGAAC TTAGCCCGGA ATAAAATTGC CATTATTCAC 
CCCAACGCTT TCTCTACGTT GCCGTCTCTA ATAAAGCTGG ACCTATCATC CAACCTCCTT 
TCGTCTTTCC CTGTGACTGG GTTACACGGT TTAACTCACT TAAAATTAAC GGGGAACCGC 
GCCTTGCAGA GCCTGATACC ATCTGCAAAC TTCCCGGAGC TCAAGATTAT AGAAATGCCA 
TACGCTTACC AGTGCTGTGC ATTTGGGGGG TGTGAGAATG TCTATAAAAT TCCTAATCAA 
TGGAACAAAG ATGACAGCAG CAGTGTGGAC GACCTTCGTA AGAAAGATGC TGGCTTATTT 
CAAGTTCAAG ATGAGCGGGA CCTTGAAGAC TTCCTACTTG ACTTTGAGGA AGACCTGAAG 
GTTCTTCACT CCGTGCAGTG CTCACCTCCC CCAGGTCCCT TCAAGCCCTG TGAGCACCTG 
TTTGGTAGCT GGCTGATCCG AATCGGGGTG TGGACCACAG CAGTCCTGGC GCTTTCCTGC 
AATGCCTTGG TGGCTTTCAC AGTGTTCAGA ACCCCTCTGT ACATCTCTTC CATAAAACTG 
CTAATCGGGG TAATTGCGGT AGTGGACATA CTCATGGGGG TCTCCAGTGC AATTCTGGCT 
GTCGTGGACA CGTTCACTTT TGGCAGTTTT GCTCAACACG GTGCATGGTG GGAAGGTGGA 
ATTGGCTGCC AAATTGTTGG TTTTTTGTCC ATTTTTGCTT CCGAATCATC GGTCTTCCTG 
CTCACTCTGG CGGCACTGGA ACGTGGTTTT TCTGTCAAGT GCTCTTCAAA GTTTGAAATG 
AAAGCTCCCC TTTCTAGCCT GAAAGCGATC ATTTTGCTGT GTGTCCTCTT GGCCCTGACC 
ATTGCCACCG TCCCCTTGCT AGGAGGCAGT GAGTACAATG CCTCTCCCCT CTGCCTGCCC 
TTACCCTTTG GGGAGCCCAG CACCACAGGC TACATGGTGG CTCTCGTATT GCTCAACTCC 
CTCTGTTTCC TCATAATGAC CATTGCCTAT ACAAGGCTCT ACTGCAGTTT GGAGAAGGGA 
GAGCTGGAGA ACCTTTGGGA CTGTTCTATG GTGAAGCACA CTGCTCTGTT GCTCTTCACC 
AACTGTATCC TTTACTGCCC CGTGGCTTTC TTATCCTTCT CCTCTTTGCT AAACCTCACG 
TTTATCAGTC CCGAAGTAAT TAAATTTATA CTTCTAGTGA TTGTCCCACT TCCGGCATGC 
CTCAACCCCC TTCTCTACAT TGTCTTCAAC CCCCATTTTA AGGAGGATAT GGGCAGCCTG 
GGAAAGCAAA CCCGTTTCTG GACAAGAGCA AAACACCCAA GTCTGCTGTC CATTAACTCG 
GACGATGTTG AGAAACGGTC CTGTGACTCA ACCCAAGCCT TAGTATCCTT TACCCACGCC 
AGCATAGCCT ATGACTTGCC TTCCGATTCC GGGTCATCAC CAGCTTACCC AATGACTGAA 
AGCTGTCACC TCTCTTCAGT TGCATTTGTC CCATGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450229.1
CDS	596..3097
Translation	MNNISQLPASLLHRLRFLEELRLAGNALTHIPKGAFAGLHSLKVLMLQNNQLRQVPEEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTDVPVQAFRSLSALQAMTLALNKIHHIADHAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIKTLSNLKELGFHSNNIRSIPERAFVGNPSLITIHFYDNPIQFVGISAFQHLPELRTLTLNGASQITEFPDLTGTATLESLTLTGAKISSLPQTVCDQLPNLQVLDLSYNLLEDLPSLSGCQKLQKIDLRHNEIYEIKGGTFQQLFNLRSLNLARNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPVTGLHGLTHLKLTGNRALQSLIPSANFPELKIIEMPYAYQCCAFGGCENVYKIPNQWNKDDSSSVDDLRKKDAGLFQVQDERDLEDFLLDFEEDLKVLHSVQCSPPPGPFKPCEHLFGSWLIRIGVWTTAVLALSCNALVAFTVFRTPLYISSIKLLIGVIAVVDILMGVSSAILAVVDTFTFGSFAQHGAWWEGGIGCQIVGFLSIFASESSVFLLTLAALERGFSVKCSSKFEMKAPLSSLKAIILLCVLLALTIATVPLLGGSEYNASPLCLPLPFGEPSTTGYMVALVLLNSLCFLIMTIAYTRLYCSLEKGELENLWDCSMVKHTALLLFTNCILYCPVAFLSFSSLLNLTFISPEVIKFILLVIVPLPACLNPLLYIVFNPHFKEDMGSLGKQTRFWTRAKHPSLLSINSDDVEKRSCDSTQALVSFTHASIAYDLPSDSGSSPAYPMTESCHLSSVAFVPCL

Target ORF information:

RefSeq Version	XM_017594740.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat containing G protein coupled receptor 5 (Lgr5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594740.1

ORF Insert Sequence:

ATGAACAACA TCAGTCAGCT ACCTGCCAGT CTCCTGCATC GCCTCCGCTT CCTAGAAGAG 
TTGCGTCTTG CTGGAAATGC TTTGACACAC ATTCCCAAGG GAGCATTCGC AGGCCTTCAC 
AGTCTCAAAG TTCTCATGCT GCAGAATAAC CAGCTGAGAC AGGTTCCGGA GGAAGCGCTA 
CAGAACCTGA GAAGCCTTCA GTCCCTGCGC TTGGATGCCA ACCACATCAG CTACGTGCCA 
CCCAGCTGTT TCAGTGGTCT GCACTCCCTG AGGCACCTGT GGCTAGATGA CAACGCTCTC 
ACAGATGTCC CTGTCCAGGC TTTCAGAAGT TTATCAGCCC TGCAAGCCAT GACCTTGGCC 
CTGAACAAAA TACACCACAT AGCAGACCAT GCCTTTGGAA ACCTCTCCAG CCTCGTGGTT 
CTGCATCTCC ATAATAATAG AATCCACTCC CTGGGAAAGA AATGCTTTGA TGGACTCCAC 
AGCCTGGAGA CTTTAGATTT AAATTATAAT AACCTTGACG AATTCCCCAC TGCAATCAAG 
ACACTCTCCA ACCTCAAGGA ACTAGGATTC CACAGCAACA ATATCAGATC AATACCAGAG 
CGAGCATTCG TAGGCAACCC TTCTCTTATC ACAATACATT TCTATGACAA TCCCATCCAG 
TTTGTTGGAA TCTCTGCTTT TCAACACTTG CCTGAACTAA GAACGCTGAC TTTGAATGGT 
GCCTCGCAAA TTACTGAATT CCCTGACTTA ACGGGGACCG CAACCCTGGA GAGCCTGACT 
TTAACTGGAG CAAAGATCTC GTCTCTTCCC CAAACCGTCT GTGATCAGTT ACCTAATCTC 
CAAGTGCTAG ATTTGTCTTA CAACCTGCTC GAAGACTTAC CCAGTTTGTC GGGCTGCCAA 
AAGCTTCAGA AAATTGACCT GCGGCATAAT GAAATCTATG AAATTAAAGG TGGCACTTTT 
CAGCAGTTGT TCAACCTCCG ATCTCTGAAC TTAGCCCGGA ATAAAATTGC CATTATTCAC 
CCCAACGCTT TCTCTACGTT GCCGTCTCTA ATAAAGCTGG ACCTATCATC CAACCTCCTT 
TCGTCTTTCC CTGTGACTGG GTTACACGGT TTAACTCACT TAAAATTAAC GGGGAACCGC 
GCCTTGCAGA GCCTGATACC ATCTGCAAAC TTCCCGGAGC TCAAGATTAT AGAAATGCCA 
TACGCTTACC AGTGCTGTGC ATTTGGGGGG TGTGAGAATG TCTATAAAAT TCCTAATCAA 
TGGAACAAAG ATGACAGCAG CAGTGTGGAC GACCTTCGTA AGAAAGATGC TGGCTTATTT 
CAAGTTCAAG ATGAGCGGGA CCTTGAAGAC TTCCTACTTG ACTTTGAGGA AGACCTGAAG 
GTTCTTCACT CCGTGCAGTG CTCACCTCCC CCAGGTCCCT TCAAGCCCTG TGAGCACCTG 
TTTGGTAGCT GGCTGATCCG AATCGGGGTG TGGACCACAG CAGTCCTGGC GCTTTCCTGC 
AATGCCTTGG TGGCTTTCAC AGTGTTCAGA ACCCCTCTGT ACATCTCTTC CATAAAACTG 
CTAATCGGGG TAATTGCGGT AGTGGACATA CTCATGGGGG TCTCCAGTGC AATTCTGGCT 
GTCGTGGACA CGTTCACTTT TGGCAGTTTT GCTCAACACG GTGCATGGTG GGAAGGTGGA 
ATTGGCTGCC AAATTGTTGG TTTTTTGTCC ATTTTTGCTT CCGAATCATC GGTCTTCCTG 
CTCACTCTGG CGGCACTGGA ACGTGGTTTT TCTGTCAAGT GCTCTTCAAA GTTTGAAATG 
AAAGCTCCCC TTTCTAGCCT GAAAGCGATC ATTTTGCTGT GTGTCCTCTT GGCCCTGACC 
ATTGCCACCG TCCCCTTGCT AGGAGGCAGT GAGTACAATG CCTCTCCCCT CTGCCTGCCC 
TTACCCTTTG GGGAGCCCAG CACCACAGGC TACATGGTGG CTCTCGTATT GCTCAACTCC 
CTCTGTTTCC TCATAATGAC CATTGCCTAT ACAAGGCTCT ACTGCAGTTT GGAGAAGGGA 
GAGCTGGAGA ACCTTTGGGA CTGTTCTATG GTGAAGCACA CTGCTCTGTT GCTCTTCACC 
AACTGTATCC TTTACTGCCC CGTGGCTTTC TTATCCTTCT CCTCTTTGCT AAACCTCACG 
TTTATCAGTC CCGAAGTAAT TAAATTTATA CTTCTAGTGA TTGTCCCACT TCCGGCATGC 
CTCAACCCCC TTCTCTACAT TGTCTTCAAC CCCCATTTTA AGGAGGATAT GGGCAGCCTG 
GGAAAGCAAA CCCGTTTCTG GACAAGAGCA AAACACCCAA GTCTGCTGTC CATTAACTCG 
GACGATGTTG AGAAACGGTC CTGTGACTCA ACCCAAGCCT TAGTATCCTT TACCCACGCC 
AGCATAGCCT ATGACTTGCC TTCCGATTCC GGGTCATCAC CAGCTTACCC AATGACTGAA 
AGCTGTCACC TCTCTTCAGT TGCATTTGTC CCATGTCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Influence of stress factors on intestinal epithelial injury and regeneration.
Pediatric surgery international(2017 Oct)
Lee C,Minich A,Li B,Miyake H,Seo S,Pierro A

Temporal and Spatial Expression of LGR5 After Acute Spinal Cord Injury in Adult Rats.
Neurochemical research41(10)2645-2654(2016 Oct)
Chen X,Hao J,Fu T,Liu J,Yu M,He S,Qian R,Zhang F

Targeting LGR5+ cells with an antibody-drug conjugate for the treatment of colon cancer.
Science translational medicine7(314)314ra186(2015 Nov)
Junttila MR,Mao W,Wang X,Wang BE,Pham T,Flygare J,Yu SF,Yee S,Goldenberg D,Fields C,Eastham-Anderson J,Singh M,Vij R,Hongo JA,Firestein R,Schutten M,Flagella K,Polakis P,Polson AG

Notch inhibition induces mitotically generated hair cells in mammalian cochleae via activating the Wnt pathway.
Proceedings of the National Academy of Sciences of the United States of America112(1)166-71(2015 Jan)
Li W,Wu J,Yang J,Sun S,Chai R,Chen ZY,Li H

Marker genes identify three somatic cell types in the fetal mouse ovary.
Developmental biology394(2)242-52(2014 Oct)
Rastetter RH,Bernard P,Palmer JS,Chassot AA,Chen H,Western PS,Ramsay RG,Chaboissier MC,Wilhelm D

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Lgr5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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