Rdh16 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rdh16
Entrez Gene ID	299511
Full Name	retinol dehydrogenase 16 (all-trans)
Synonyms	Rdh2,RoDHII
General protein information	Preferred Names retinol dehydrogenase 16 (all-trans) Names retinol dehydrogenase 2 29 kDa protein RODH II retinol dehydrogenase 2-like retinol dehydrogenase type 2 retinol dehydrogenase type II (RODH II)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	a short-chain dehydrogenase/reductase that catalyzes the conversion of retinol to retinal, the first step in retinoic acid synthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199208.1	NP_954678.1	retinol dehydrogenase 16 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rdh19	NP_671755.2
Rattus norvegicus (Norway rat)	Rdh16	NP_954678.1
Xenopus tropicalis (tropical clawed frog)	hsd17b6	NP_001011296.1
Xenopus tropicalis (tropical clawed frog)	LOC100498221	XP_002939751.1
Pan troglodytes (chimpanzee)	RDH16	XP_522604.1
	RDH16	XP_001115484.1
Mus musculus (house mouse)	BC089597	NP_663399.2
Xenopus tropicalis (tropical clawed frog)	rdh7	NP_001017189.1
Xenopus tropicalis (tropical clawed frog)	LOC100498066	XP_002939750.1
Mus musculus (house mouse)	Rdh16	NP_033066.1
Mus musculus (house mouse)	Rdh9	NP_694773.1
Danio rerio (zebrafish)	dhrs9	NP_955903.1
Xenopus tropicalis (tropical clawed frog)	MGC89248	NP_001004956.1
Xenopus tropicalis (tropical clawed frog)	rdh16	NP_001107359.1
Homo sapiens (human)	RDH16	NP_003699.3
Canis lupus familiaris (dog)	RDH16	XP_531641.2
Bos taurus (cattle)	RDH16	NP_001069264.1
Mus musculus (house mouse)	Rdh1	NP_536684.2
Rattus norvegicus (Norway rat)	LOC100365958	XP_006241574.1
Gallus gallus (chicken)	LOC776432	XP_001235955.3
Xenopus tropicalis (tropical clawed frog)	rdh7.2	NP_001107972.1
Xenopus tropicalis (tropical clawed frog)	LOC100170425	NP_001123675.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiple retinol and retinal dehydrogenases catalyze all-trans-retinoic acid biosynthesis in astrocytes.
Wang C, Kane MA, Napoli JL
The Journal of biological chemistry286(8)6542-53(2011 Feb)

The C-terminal region of cis-retinol/androgen dehydrogenase 1 (CRAD1) confers ER localization and in vivo enzymatic function.
Lid?n M, Tryggvason K, Eriksson U
Experimental cell research311(2)205-17(2005 Dec)

Combined genealogical, mapping, and expression approaches to identify spontaneously hypertensive rat hypertension candidate genes.
Hinojos CA, Boerwinkle E, Fornage M, Doris PA
Hypertension (Dallas, Tex. : 1979)45(4)698-704(2005 Apr)

Elements in the N-terminal signaling sequence that determine cytosolic topology of short-chain dehydrogenases/reductases. Studies with retinol dehydrogenase type 1 and cis-retinol/androgen dehydrogenase type 1.
Zhang M, Hu P, Napoli JL
The Journal of biological chemistry279(49)51482-9(2004 Dec)

Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4.
Lapshina EA, Belyaeva OV, Chumakova OV, Kedishvili NY
Biochemistry42(3)776-84(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rdh16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rdh16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42503	NM_199208.1 Latest version!	Rattus norvegicus retinol dehydrogenase 16 (Rdh16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rdh16 ( NM_199208.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954678.1 Rattus norvegicus retinol dehydrogenase 16 (Rdh16), mRNA.

Price & Availability↑

CloneID	ORa42503
Clone ID Related Accession (Same CDS sequence)	NM_199208.1
Accession Version	NM_199208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	retinol dehydrogenase 16 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U33500.1. On Jan 1, 2004 this sequence version replaced XM_216817.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC062000.1 [ECO:0000331] ##Evidence-Data-END##

ATGTGGCTGT ACCTGCTGGC ACTGGTGGGC CTGTGGAACC TCCTGCGCTT GTTCAGGGAG 
AGGAAGGTGG TAAGCCATCT CCAAGACAAA TATGTCTTCA TCACGGGCTG TGACTCAGGC 
TTTGGGAACC TGCTGGCCAG ACAGCTGGAC AGGAGAGGCA TGAGGGTGCT GGCTGCATGT 
CTGACTGAGA AGGGAGCCGA GCAGCTGAGG AGCAAGACAT CGGACAGGCT GGAGACAGTG 
ATCCTTGATG TCACCAAGAC AGAGAGTATT GTGGCAGCCA CTCAGTGGGT GAAGGAGCGA 
GTTGGGAACA CAGGACTCTG GGGCCTGGTC AACAATGCTG GCATCTCCGG CCACCTGGGT 
CCCAATGAGT GGATGAACAA ACAGAACATT GCAAGTGTGC TGGATGTGAA CCTGTTGGGC 
ATGATCGAGG TGACTCTGAG CACGGTGCCC TTAGTGAGGA AGGCGAGGGG CCGTGTGGTC 
AACGTTGCCA GCATCGCAGG CCGACTGTCT TTTTGTGGTG GTGGTTACTG CATCTCCAAG 
TATGGTGTAG AGGCCTTCTC AGACTCCCTC AGGAGAGAGC TCTCCTATTT TGGGGTCAAG 
GTGGCTATTG TAGAGCCTGG CTTCTTCAGG ACTGATGTGA CCAATGGTGT CACATTATCA 
TCAAACTTCC AGATGCTGTG GGACCAGACC AGCTCAGAAG TCAGGGAAGT CTATGGCGAG 
AACTACCTGG CATCTTATCT GAAAATGCTA AATGGATTGG ACCAGAGGTG CAACAAAGAT 
CTGTCTCTGG TGACTGACTG CATGGAGCAC GCCCTGACTT CCTGTCACCC TCGCACCCGG 
TACTCAGCTG GTTGGGATGC CAAGTTCTTC TACCTCCCCA TGAGCTATCT GCCCACCTTT 
CTTGTGGATG CCCTTTTCTA CTGGACTTCT CCAAAGCCTG AGAAAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954678.1
CDS	229..1182
Translation	MWLYLLALVGLWNLLRLFRERKVVSHLQDKYVFITGCDSGFGNLLARQLDRRGMRVLAACLTEKGAEQLRSKTSDRLETVILDVTKTESIVAATQWVKERVGNTGLWGLVNNAGISGHLGPNEWMNKQNIASVLDVNLLGMIEVTLSTVPLVRKARGRVVNVASIAGRLSFCGGGYCISKYGVEAFSDSLRRELSYFGVKVAIVEPGFFRTDVTNGVTLSSNFQMLWDQTSSEVREVYGENYLASYLKMLNGLDQRCNKDLSLVTDCMEHALTSCHPRTRYSAGWDAKFFYLPMSYLPTFLVDALFYWTSPKPEKAL

Target ORF information:

RefSeq Version	NM_199208.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus retinol dehydrogenase 16 (Rdh16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199208.1

ORF Insert Sequence:

ATGTGGCTGT ACCTGCTGGC ACTGGTGGGC CTGTGGAACC TCCTGCGCTT GTTCAGGGAG 
AGGAAGGTGG TAAGCCATCT CCAAGACAAA TATGTCTTCA TCACGGGCTG TGACTCAGGC 
TTTGGGAACC TGCTGGCCAG ACAGCTGGAC AGGAGAGGCA TGAGGGTGCT GGCTGCATGT 
CTGACTGAGA AGGGAGCCGA GCAGCTGAGG AGCAAGACAT CGGACAGGCT GGAGACAGTG 
ATCCTTGATG TCACCAAGAC AGAGAGTATT GTGGCAGCCA CTCAGTGGGT GAAGGAGCGA 
GTTGGGAACA CAGGACTCTG GGGCCTGGTC AACAATGCTG GCATCTCCGG CCACCTGGGT 
CCCAATGAGT GGATGAACAA ACAGAACATT GCAAGTGTGC TGGATGTGAA CCTGTTGGGC 
ATGATCGAGG TGACTCTGAG CACGGTGCCC TTAGTGAGGA AGGCGAGGGG CCGTGTGGTC 
AACGTTGCCA GCATCGCAGG CCGACTGTCT TTTTGTGGTG GTGGTTACTG CATCTCCAAG 
TATGGTGTAG AGGCCTTCTC AGACTCCCTC AGGAGAGAGC TCTCCTATTT TGGGGTCAAG 
GTGGCTATTG TAGAGCCTGG CTTCTTCAGG ACTGATGTGA CCAATGGTGT CACATTATCA 
TCAAACTTCC AGATGCTGTG GGACCAGACC AGCTCAGAAG TCAGGGAAGT CTATGGCGAG 
AACTACCTGG CATCTTATCT GAAAATGCTA AATGGATTGG ACCAGAGGTG CAACAAAGAT 
CTGTCTCTGG TGACTGACTG CATGGAGCAC GCCCTGACTT CCTGTCACCC TCGCACCCGG 
TACTCAGCTG GTTGGGATGC CAAGTTCTTC TACCTCCCCA TGAGCTATCT GCCCACCTTT 
CTTGTGGATG CCCTTTTCTA CTGGACTTCT CCAAAGCCTG AGAAAGCTCT CTGA