Srsf12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srsf12
Entrez Gene ID	297962
Full Name	serine and arginine rich splicing factor 12
Synonyms	RGD1561162,Srrp,Srrp35
General protein information	Preferred Names serine and arginine rich splicing factor 12 Names serine/arginine-rich splicing factor 12 35 kDa SR repressor protein 35 kDa serine-arginine repressor serine-arginine repressor protein (35 kDa) serine-arginine repressor protein 35
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135711.1	NP_001129183.1	serine/arginine-rich splicing factor 12

Pathways from BioSystems

Homologies

Homo sapiens (human)	SRSF12	NP_542781.3
Rattus norvegicus (Norway rat)	Srsf12	NP_001129183.1
Xenopus tropicalis (tropical clawed frog)	srsf12	NP_001007946.1
Bos taurus (cattle)	SRSF12	XP_002690241.1
Pan troglodytes (chimpanzee)	SRSF12	XP_518634.2

Gene Ontology
Bibliography

Related articles in PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Nucleic acids research35(12)3928-44(2007)

Serine-arginine (SR) protein-like factors that antagonize authentic SR proteins and regulate alternative splicing.
Cowper AE, C?ceres JF, Mayeda A, Screaton GR
The Journal of biological chemistry276(52)48908-14(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Srsf12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srsf12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04022	NM_001135711.1 Latest version!	Rattus norvegicus serine and arginine rich splicing factor 12 (Srsf12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srsf12 ( NM_001135711.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001129183.1 Rattus norvegicus serine and arginine rich splicing factor 12 (Srsf12), mRNA.

Price & Availability↑

CloneID	ORa04022
Clone ID Related Accession (Same CDS sequence)	NM_001135711.1
Accession Version	NM_001135711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	serine/arginine-rich splicing factor 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07047799.1 and AABR07047800.1. On Oct 8, 2008 this sequence version replaced XM_216364.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CB585612.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCGCT ACACCAGGCC CCCCAACACC TCCCTGTTCG TCAGGAACGT CGCAGACGCC 
ACCAGGCCCG AGGACTTACG CCGTGAGTTT GGCCGATACG GCCCCATAGT AGACGTTTAT 
ATCCCGCTGG ACTTCTACAG TCGCCGCCCC AGAGGATTCG CTTATGTTCA GTTTGAAGAT 
GTTCGTGATG CCGAAGATGC ACTTTACAAC CTCAACAGGA AGTGGGTATG TGGCCGGCAA 
ATTGAGATAC AGTTTGCGCA AGGTGACCGC AAAACACCAG GTCAAATGAA GTCAAAAGAG 
CGTCACCTGT GCTCCCCGAG TGACCACAGG AGATCCAGAA GCCCCAGCCA GAGGAGATCT 
CGAAGCCGGA GTTCCTCATG GGGAAGAGAC CGGAGGCGCT CCGATAGCCT GAAAGAGTCT 
CGACACAGGC GATCTTCTTA CAGTCAGTCT AAATCTCGTT CCAAATCACT ACCCAGGCAG 
TCTACCTCAG CGAGGCAGTC AAGAACACCA CGAAGGAATT CTGGATCTAG AGGACGATCA 
AGATCCAAGT CGCTACCGAA AAGGTCCAAA TCGATGGAAA AGTCACAGTC ACGCTCGCCT 
CAAAAGCAGA CTGTCTCGGG AGCAAAACCC AGATCGCACG GACGGCACTG TGACTCCATA 
GCAAGATCCC CATGCAAGTC TCCCAGAGGG TACGCCAGTT CGGGGTCGAA GACACAGACA 
ACGAAACATT CTCACTTCCG GTCACACTCC AGATCCCGGA GCTACCATCA TAAAAACAGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129183.1
CDS	1..786
Translation	MSRYTRPPNTSLFVRNVADATRPEDLRREFGRYGPIVDVYIPLDFYSRRPRGFAYVQFEDVRDAEDALYNLNRKWVCGRQIEIQFAQGDRKTPGQMKSKERHLCSPSDHRRSRSPSQRRSRSRSSSWGRDRRRSDSLKESRHRRSSYSQSKSRSKSLPRQSTSARQSRTPRRNSGSRGRSRSKSLPKRSKSMEKSQSRSPQKQTVSGAKPRSHGRHCDSIARSPCKSPRGYASSGSKTQTTKHSHFRSHSRSRSYHHKNSW

Target ORF information:

RefSeq Version	NM_001135711.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine and arginine rich splicing factor 12 (Srsf12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135711.1

ORF Insert Sequence:

ATGTCTCGCT ACACCAGGCC CCCCAACACC TCCCTGTTCG TCAGGAACGT CGCAGACGCC 
ACCAGGCCCG AGGACTTACG CCGTGAGTTT GGCCGATACG GCCCCATAGT AGACGTTTAT 
ATCCCGCTGG ACTTCTACAG TCGCCGCCCC AGAGGATTCG CTTATGTTCA GTTTGAAGAT 
GTTCGTGATG CCGAAGATGC ACTTTACAAC CTCAACAGGA AGTGGGTATG TGGCCGGCAA 
ATTGAGATAC AGTTTGCGCA AGGTGACCGC AAAACACCAG GTCAAATGAA GTCAAAAGAG 
CGTCACCTGT GCTCCCCGAG TGACCACAGG AGATCCAGAA GCCCCAGCCA GAGGAGATCT 
CGAAGCCGGA GTTCCTCATG GGGAAGAGAC CGGAGGCGCT CCGATAGCCT GAAAGAGTCT 
CGACACAGGC GATCTTCTTA CAGTCAGTCT AAATCTCGTT CCAAATCACT ACCCAGGCAG 
TCTACCTCAG CGAGGCAGTC AAGAACACCA CGAAGGAATT CTGGATCTAG AGGACGATCA 
AGATCCAAGT CGCTACCGAA AAGGTCCAAA TCGATGGAAA AGTCACAGTC ACGCTCGCCT 
CAAAAGCAGA CTGTCTCGGG AGCAAAACCC AGATCGCACG GACGGCACTG TGACTCCATA 
GCAAGATCCC CATGCAAGTC TCCCAGAGGG TACGCCAGTT CGGGGTCGAA GACACAGACA 
ACGAAACATT CTCACTTCCG GTCACACTCC AGATCCCGGA GCTACCATCA TAAAAACAGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Nucleic acids research35(12)3928-44(2007)
Chen YI,Moore RE,Ge HY,Young MK,Lee TD,Stevens SW

Serine-arginine (SR) protein-like factors that antagonize authentic SR proteins and regulate alternative splicing.
The Journal of biological chemistry276(52)48908-14(2001 Dec)
Cowper AE,C?ceres JF,Mayeda A,Screaton GR