Ankra2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankra2
Entrez Gene ID	294679
Full Name	ankyrin repeat family A member 2
Synonyms	ANKRA
General protein information	Preferred Names ankyrin repeat family A member 2 Names ankyrin repeat family A protein 2 SLO-interacting ankyrin-containing protein ankyrin repeat, family A (RFXANK-like), 2 ankyrin-repeat family A protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq31

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257186.2	XP_006257248.1	ankyrin repeat family A protein 2 isoform X1
NM_207595.1	NP_997478.1	ankyrin repeat family A protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ANKRA2	XP_001151939.1
Homo sapiens (human)	ANKRA2	NP_075526.1
Bos taurus (cattle)	ANKRA2	NP_001039754.1
Mus musculus (house mouse)	Ankra2	NP_075961.1
Rattus norvegicus (Norway rat)	Ankra2	NP_997478.1
Gallus gallus (chicken)	ANKRA2	NP_001006565.1
Xenopus tropicalis (tropical clawed frog)	ankra2	NP_001006692.1
	ANKRA2	XP_001101235.1
Canis lupus familiaris (dog)	ANKRA2	XP_535273.1
Danio rerio (zebrafish)	ankra2	NP_998294.1

Gene Ontology
Bibliography

Related articles in PubMed

Ankyrin repeats of ANKRA2 recognize a PxLPxL motif on the 3M syndrome protein CCDC8.
Nie J, Xu C, Jin J, Aka JA, Tempel W, Nguyen V, You L, Weist R, Min J, Pawson T, Yang XJ
Structure (London, England : 1993)23(4)700-12(2015 Apr)

Sequence-specific recognition of a PxLPxI/L motif by an ankyrin repeat tumbler lock.
Xu C, Jin J, Bian C, Lam R, Tian R, Weist R, You L, Nie J, Bochkarev A, Tempel W, Tan CS, Wasney GA, Vedadi M, Gish GD, Arrowsmith CH, Pawson T, Yang XJ, Min J
Science signaling5(226)ra39(2012 May)

Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome.
Vaz Meirelles G, Ferreira Lanza DC, da Silva JC, Santana Bernachi J, Paes Leme AF, Kobarg J
Journal of proteome research9(12)6298-316(2010 Dec)

Class II histone deacetylases confer signal responsiveness to the ankyrin-repeat proteins ANKRA2 and RFXANK.
McKinsey TA, Kuwahara K, Bezprozvannaya S, Olson EN
Molecular biology of the cell17(1)438-47(2006 Jan)

Identification and functional characterization of ankyrin-repeat family protein ANKRA as a protein interacting with BKCa channel.
Lim HH, Park CS
Molecular biology of the cell16(3)1013-25(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ANKRA could modulate the excitability of neurons by binding directly to endogenous BKCa channel and altering its gating kinetics in a calcium-dependent manner
Title: Identification and functional characterization of ankyrin-repeat family protein ANKRA as a protein interacting with BKCa channel.

The following Ankra2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankra2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05579	NM_207595.1 Latest version!	Rattus norvegicus ankyrin repeat family A member 2 (Ankra2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa15053	XM_006257186.2 Latest version!	Rattus norvegicus ankyrin repeat family A member 2 (Ankra2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankra2 ( NM_207595.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997478.1 Rattus norvegicus ankyrin repeat family A member 2 (Ankra2), mRNA.

Price & Availability↑

CloneID	ORa05579
Clone ID Related Accession (Same CDS sequence)	NM_207595.1
Accession Version	NM_207595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat family A protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY566568.1. On Aug 30, 2004 this sequence version replaced XM_215457.2. ##Evidence-Data-START## Transcript exon combination :: AY566568.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTACAT CTGCAAATCT GGACATTGGG GCCCAACTGG TCGTGGAAGA GTGTCCCAGC 
AGCTATGGCC TCTCTGGGAT GCCAGACATC AAAATAGAGC ACCAGCCAGA CCCAAACTCA 
GATGAAGGGT CAGCTCAGGG AGTCGCCATG GGGATGAAGT TCATACTGCC AAACCGATTT 
GATATGAACG TGTGCTCTCG CTTTGTGAAG TCCTTAAACG AAGAGGATAG TAAAAATATT 
CAAGATCAAG TTAACTCTGA CCTGGAGGTG GCGTCTGTCT TATTTAAAGC TGAATGCAAT 
ATCCATACCT CTCCTTCTCC TGGAATTCAA GTAAGGCACG TCTACACACC CTCTACAACT 
AAACATTTCT CACCCATAAA ACAGTCAACT ACTTTAACCA ACAAGCACAG GGGAAACGAG 
GTCTCCACCA CACCTCTGTT AGCAAATTCT CTGTCTGTTC ACCAGTTGGC AGCTCAGGGA 
GAGATGCTCT ACCTGGCTAC TCGTATTGAA CAAGAAAATG TTATCAATCA CACGGATGAA 
GAAGGATTTA CTCCCCTGAT GTGGGCTGCG GCACACGGGC AAATAGCTGT GGTAGAATTC 
CTCCTTCAGA ACGGTGCAGA TCCCCAGCTC TTAGGAAAAG GTCGAGAAAG TGCCCTGTCG 
TTGGCCTGCA GTAAGGGCTA CACAGACATT GTCAAAATGC TGCTAGACTG TGGAGTTGAC 
GTAAATGAGT ATGACTGGAA CGGAGGCACC CCTTTGCTTT ATGCTGTCCA CGGGAACCAC 
GGGAAGTGCG TGAAGATGCT GTTGGAAAAT GGAGCTGACC CAACAATTGA AACAGACTCT 
GGCTATAATT CTATGGACTT GGCTGTAGCT CTGGGCTACA GAAGTGTTCA ACAGGTCATT 
GAGTCACACC TGCTGAAGCT GCTTCAGAAC ATCAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997478.1
CDS	217..1158
Translation	MATSANLDIGAQLVVEECPSSYGLSGMPDIKIEHQPDPNSDEGSAQGVAMGMKFILPNRFDMNVCSRFVKSLNEEDSKNIQDQVNSDLEVASVLFKAECNIHTSPSPGIQVRHVYTPSTTKHFSPIKQSTTLTNKHRGNEVSTTPLLANSLSVHQLAAQGEMLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKMLLDCGVDVNEYDWNGGTPLLYAVHGNHGKCVKMLLENGADPTIETDSGYNSMDLAVALGYRSVQQVIESHLLKLLQNIKE

Target ORF information:

RefSeq Version	NM_207595.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat family A member 2 (Ankra2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207595.1

ORF Insert Sequence:

ATGGCTACAT CTGCAAATCT GGACATTGGG GCCCAACTGG TCGTGGAAGA GTGTCCCAGC 
AGCTATGGCC TCTCTGGGAT GCCAGACATC AAAATAGAGC ACCAGCCAGA CCCAAACTCA 
GATGAAGGGT CAGCTCAGGG AGTCGCCATG GGGATGAAGT TCATACTGCC AAACCGATTT 
GATATGAACG TGTGCTCTCG CTTTGTGAAG TCCTTAAACG AAGAGGATAG TAAAAATATT 
CAAGATCAAG TTAACTCTGA CCTGGAGGTG GCGTCTGTCT TATTTAAAGC TGAATGCAAT 
ATCCATACCT CTCCTTCTCC TGGAATTCAA GTAAGGCACG TCTACACACC CTCTACAACT 
AAACATTTCT CACCCATAAA ACAGTCAACT ACTTTAACCA ACAAGCACAG GGGAAACGAG 
GTCTCCACCA CACCTCTGTT AGCAAATTCT CTGTCTGTTC ACCAGTTGGC AGCTCAGGGA 
GAGATGCTCT ACCTGGCTAC TCGTATTGAA CAAGAAAATG TTATCAATCA CACGGATGAA 
GAAGGATTTA CTCCCCTGAT GTGGGCTGCG GCACACGGGC AAATAGCTGT GGTAGAATTC 
CTCCTTCAGA ACGGTGCAGA TCCCCAGCTC TTAGGAAAAG GTCGAGAAAG TGCCCTGTCG 
TTGGCCTGCA GTAAGGGCTA CACAGACATT GTCAAAATGC TGCTAGACTG TGGAGTTGAC 
GTAAATGAGT ATGACTGGAA CGGAGGCACC CCTTTGCTTT ATGCTGTCCA CGGGAACCAC 
GGGAAGTGCG TGAAGATGCT GTTGGAAAAT GGAGCTGACC CAACAATTGA AACAGACTCT 
GGCTATAATT CTATGGACTT GGCTGTAGCT CTGGGCTACA GAAGTGTTCA ACAGGTCATT 
GAGTCACACC TGCTGAAGCT GCTTCAGAAC ATCAAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankra2 ( XM_006257186.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257248.1 Rattus norvegicus ankyrin repeat family A member 2 (Ankra2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa15053
Clone ID Related Accession (Same CDS sequence)	XM_006257186.2
Accession Version	XM_006257186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat family A protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006257186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTACC TGGCTACTCG TATTGAACAA GAAAATGTTA TCAATCACAC GGATGAAGAA 
GGATTTACTC CCCTGATGTG GGCTGCGGCA CACGGGCAAA TAGCTGTGGT AGAATTCCTC 
CTTCAGAACG GTGCAGATCC CCAGCTCTTA GGAAAAGGTC GAGAAAGTGC CCTGTCGTTG 
GCCTGCAGTA AGGGCTACAC AGACATTGTC AAAATGCTGC TAGACTGTGG AGTTGACGTA 
AATGAGTATG ACTGGAACGG AGGCACCCCT TTGCTTTATG CTGTCCACGG GAACCACGGG 
AAGTGCGTGA AGATGCTGTT GGAAAATGGA GCTGACCCAA CAATTGAAAC AGACTCTGGC 
TATAATTCTA TGGACTTGGC TGTAGCTCTG GGCTACAGAA GTGTTCAACA GGTCATTGAG 
TCACACCTGC TGAAGCTGCT TCAGAACATC AAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257248.1
CDS	506..964
Translation	MLYLATRIEQENVINHTDEEGFTPLMWAAAHGQIAVVEFLLQNGADPQLLGKGRESALSLACSKGYTDIVKMLLDCGVDVNEYDWNGGTPLLYAVHGNHGKCVKMLLENGADPTIETDSGYNSMDLAVALGYRSVQQVIESHLLKLLQNIKE

Target ORF information:

RefSeq Version	XM_006257186.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat family A member 2 (Ankra2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257186.2

ORF Insert Sequence:

ATGCTCTACC TGGCTACTCG TATTGAACAA GAAAATGTTA TCAATCACAC GGATGAAGAA 
GGATTTACTC CCCTGATGTG GGCTGCGGCA CACGGGCAAA TAGCTGTGGT AGAATTCCTC 
CTTCAGAACG GTGCAGATCC CCAGCTCTTA GGAAAAGGTC GAGAAAGTGC CCTGTCGTTG 
GCCTGCAGTA AGGGCTACAC AGACATTGTC AAAATGCTGC TAGACTGTGG AGTTGACGTA 
AATGAGTATG ACTGGAACGG AGGCACCCCT TTGCTTTATG CTGTCCACGG GAACCACGGG 
AAGTGCGTGA AGATGCTGTT GGAAAATGGA GCTGACCCAA CAATTGAAAC AGACTCTGGC 
TATAATTCTA TGGACTTGGC TGTAGCTCTG GGCTACAGAA GTGTTCAACA GGTCATTGAG 
TCACACCTGC TGAAGCTGCT TCAGAACATC AAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ankyrin repeats of ANKRA2 recognize a PxLPxL motif on the 3M syndrome protein CCDC8.
Structure (London, England : 1993)23(4)700-12(2015 Apr)
Nie J,Xu C,Jin J,Aka JA,Tempel W,Nguyen V,You L,Weist R,Min J,Pawson T,Yang XJ

Sequence-specific recognition of a PxLPxI/L motif by an ankyrin repeat tumbler lock.
Science signaling5(226)ra39(2012 May)
Xu C,Jin J,Bian C,Lam R,Tian R,Weist R,You L,Nie J,Bochkarev A,Tempel W,Tan CS,Wasney GA,Vedadi M,Gish GD,Arrowsmith CH,Pawson T,Yang XJ,Min J

Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome.
Journal of proteome research9(12)6298-316(2010 Dec)
Vaz Meirelles G,Ferreira Lanza DC,da Silva JC,Santana Bernachi J,Paes Leme AF,Kobarg J

Class II histone deacetylases confer signal responsiveness to the ankyrin-repeat proteins ANKRA2 and RFXANK.
Molecular biology of the cell17(1)438-47(2006 Jan)
McKinsey TA,Kuwahara K,Bezprozvannaya S,Olson EN

Identification and functional characterization of ankyrin-repeat family protein ANKRA as a protein interacting with BKCa channel.
Molecular biology of the cell16(3)1013-25(2005 Mar)
Lim HH,Park CS