Tufm cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tufm
Entrez Gene ID	293481
Full Name	Tu translation elongation factor, mitochondrial
General protein information	Preferred Names Tu translation elongation factor, mitochondrial Names elongation factor Tu, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_006230224.1	XP_006230286.1	elongation factor Tu, mitochondrial isoform X1
XM_006230225.1	XP_006230287.1	elongation factor Tu, mitochondrial isoform X2
NM_001106295.1	NP_001099765.1	elongation factor Tu, mitochondrial precursor

Pathways from BioSystems

Homologies

	TUFM	XP_001103519.1
Homo sapiens (human)	TUFM	NP_003312.3
Saccharomyces cerevisiae (baker's yeast)	TUF1	NP_014830.1
Kluyveromyces lactis	KLLA0A04587g	XP_451201.1
Kluyveromyces lactis	KLLA0C05214g	XP_452433.1
Eremothecium gossypii	AGOS_AAR143W	NP_982685.1
Magnaporthe oryzae (rice blast fungus)	MGG_08162	XP_003715132.1
Canis lupus familiaris (dog)	TUFM	XP_536924.3
Bos taurus (cattle)	TUFM	NP_776632.1
Rattus norvegicus (Norway rat)	Tufm	NP_001099765.1
Danio rerio (zebrafish)	tufm	NP_001013523.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006996	XP_308774.4
Neurospora crassa	NCU03737	XP_961513.1
Pan troglodytes (chimpanzee)	TUFM	XP_510904.2
Drosophila melanogaster (fruit fly)	EfTuM	NP_001163144.1
Schizosaccharomyces pombe (fission yeast)	tuf1	NP_595746.1
Arabidopsis thaliana (thale cress)	AT4G02930	NP_192202.1
Oryza sativa (rice)	Os03g0851100	NP_001051912.1
Mus musculus (house mouse)	Tufm	NP_766333.1
Caenorhabditis elegans (roundworm)	tufm-1	NP_497623.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium.
Zhang DX, Yan H, Hu JY, Zhang JP, Teng M, Tong DL, Xiang F, Zhang Q, Fang YD, Liang GP, Huang YS
Journal of proteomics77469-79(2012 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Chronic ethanol feeding causes depression of mitochondrial elongation factor Tu in the rat liver: implications for the mitochondrial ribosome.
Weiser B, Gonye G, Sykora P, Crumm S, Cahill A
American journal of physiology. Gastrointestinal and liver physiology300(5)G815-22(2011 May)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the mitochondrial responses to severe burn, and indicate a pathway by which decreased EF-Tumt expression mediates oxidative damage in postburn myocardium.
Title: Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium.

The following Tufm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tufm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00764	NM_001106295.1 Latest version!	Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa23409	XM_006230224.1 Latest version!	Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa23410	XM_006230225.1 Latest version!	Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tufm ( NM_001106295.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099765.1 Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), mRNA.

Price & Availability↑

CloneID	ORa00764
Clone ID Related Accession (Same CDS sequence)	NM_001106295.1
Accession Version	NM_001106295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	elongation factor Tu, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473956.2. On or before Oct 4, 2007 this sequence version replaced XM_001077188.1, XM_215069.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTTTA TATCCTCAGC 
AAGGAGGAGG GTGGCCGCCA CAAACCCTTT GTATCTCATT TCATGCCCGT CATGTTCTCC 
CTGACCTGGG ACATGGCCTG TCGAGTCATC TTGCCTCCAG GGAAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099765.1
CDS	347..1705
Translation	MAAATLLRATPRFSGLCASPTPFLQGRLRPLKAPASPFLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLAKQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEQRDPELGVKSVQKLLDAVDTYIPVPTRDLEKPFLLPVESVYSIPGRGTVVTGTLERGILKKGDECELLGHNKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIQPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRVILPPGKELAMPGEDLKLSLILRQPMILEKGQRFTLRDGNKTIGTGLVTDVPAMTEEDKNIKWS

Target ORF information:

RefSeq Version	NM_001106295.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106295.1

ORF Insert Sequence:

ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGTTTA TATCCTCAGC 
AAGGAGGAGG GTGGCCGCCA CAAACCCTTT GTATCTCATT TCATGCCCGT CATGTTCTCC 
CTGACCTGGG ACATGGCCTG TCGAGTCATC TTGCCTCCAG GGAAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tufm ( XM_006230224.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230286.1 Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23409
Clone ID Related Accession (Same CDS sequence)	XM_006230224.1
Accession Version	XM_006230224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	elongation factor Tu, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TTGGTGCTCT GGTCCGAGGC 
TTAAAGCGAG AGGATTTGAG GCGTGGTTTG GTCATGGTCA AGCCAGGATC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAGGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGCCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACTTTGAG GGATGGCAAC 
AAGACCATTG GCACAGGCCT TGTCACTGAT GTACCAGCCA TGACCGAGGA GGACAAGAAC 
ATCAAATGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230286.1
CDS	342..1616
Translation	MAAATLLRATPRFSGLCASPTPFLQGRLRPLKAPASPFLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLAKQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEQRDPELGVKSVQKLLDAVDTYIPVPTRDLEKPFLLPVESVYSIPGRGTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIQPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRVILPPGKELAMPGEDLKLSLILRQPMILEKGQRFTLRDGNKTIGTGLVTDVPAMTEEDKNIKWS

Target ORF information:

RefSeq Version	XM_006230224.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230224.1

ORF Insert Sequence:

ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG CATTGAGATG 
TTCCACAAGA GCCTGGAGAG GGCTGAGGCA GGGGATAACC TTGGTGCTCT GGTCCGAGGC 
TTAAAGCGAG AGGATTTGAG GCGTGGTTTG GTCATGGTCA AGCCAGGATC CATCCAACCC 
CACCAGAAGG TGGAGGCCCA GGTTTATATC CTCAGCAAGG AGGAGGGTGG CCGCCACAAA 
CCCTTTGTAT CTCATTTCAT GCCCGTCATG TTCTCCCTGA CCTGGGACAT GGCCTGTCGA 
GTCATCTTGC CTCCAGGGAA GGAACTTGCC ATGCCTGGAG AGGACTTGAA GCTTAGCCTA 
ATCTTGCGGC AGCCCATGAT CTTAGAGAAA GGCCAACGTT TCACTTTGAG GGATGGCAAC 
AAGACCATTG GCACAGGCCT TGTCACTGAT GTACCAGCCA TGACCGAGGA GGACAAGAAC 
ATCAAATGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tufm ( XM_006230225.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230287.1 Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa23410
Clone ID Related Accession (Same CDS sequence)	XM_006230225.1
Accession Version	XM_006230225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	elongation factor Tu, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230287.1
CDS	342..1580
Translation	MAAATLLRATPRFSGLCASPTPFLQGRLRPLKAPASPFLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLAKQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEQRDPELGVKSVQKLLDAVDTYIPVPTRDLEKPFLLPVESVYSIPGRGTVVTGTLERGILKKGDECELLGHNKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIQPHQKVEAQELAMPGEDLKLSLILRQPMILEKGQRFTLRDGNKTIGTGLVTDVPAMTEEDKNIKWS

Target ORF information:

RefSeq Version	XM_006230225.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Tu translation elongation factor, mitochondrial (Tufm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230225.1

ORF Insert Sequence:

ATGGCGGCAG CGACTCTATT ACGAGCGACG CCCCGTTTCA GCGGTCTCTG CGCCAGCCCA 
ACCCCATTTC TGCAAGGTCG GCTGCGGCCG CTGAAGGCCC CGGCATCGCC CTTCTTGTGT 
CGCGGCCTAG CAGTGGAGGC CAAGAAGACT TACGTACGCG ACAAACCCCA TGTGAATGTG 
GGTACCATCG GGCATGTGGA CCATGGCAAG ACCACCCTGA CCGCAGCCAT CACGAAAATT 
CTAGCCGAGG GAGGTGGAGC TAAGTTCAAG AAGTACGAGG AGATAGACAA TGCCCCAGAG 
GAGCGAGCTC GGGGTATCAC CATCAATGCA GCTCATGTGG AGTATAGCAC TGCTGCCCGA 
CACTATGCCC ACACAGACTG CCCTGGTCAT GCAGATTATG TTAAGAATAT GATCACAGGC 
ACCGCCCCTC TGGATGGCTG CATCCTGGTG GTGGCAGCTA ATGATGGCCC CATGCCCCAG 
ACCCGAGAGC ACTTACTGCT AGCTAAGCAG ATTGGGGTAG AGCATGTTGT GGTGTATGTG 
AACAAGGCAG ATGCTGTCCA GGACTCGGAG ATGGTGGAGC TGGTAGAGCT GGAGATCCGG 
GAGCTGCTCA CTGAGTTTGG CTATAAAGGA GAGGAAACCC CAGTCATCGT AGGCTCGGCC 
CTCTGTGCTC TTGAGCAACG TGATCCTGAG CTAGGTGTGA AGTCAGTGCA GAAGCTTCTG 
GATGCTGTGG ACACTTACAT CCCAGTACCC ACCCGGGACC TGGAGAAGCC CTTTCTGCTC 
CCTGTAGAGT CAGTTTACTC TATTCCTGGC CGCGGCACAG TGGTGACAGG TACACTAGAA 
CGTGGCATTT TGAAGAAAGG AGATGAGTGT GAGTTGCTGG GACATAACAA GAACATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG AGAGGGCTGA GGCAGGGGAT 
AACCTTGGTG CTCTGGTCCG AGGCTTAAAG CGAGAGGATT TGAGGCGTGG TTTGGTCATG 
GTCAAGCCAG GATCCATCCA ACCCCACCAG AAGGTGGAGG CCCAGGAACT TGCCATGCCT 
GGAGAGGACT TGAAGCTTAG CCTAATCTTG CGGCAGCCCA TGATCTTAGA GAAAGGCCAA 
CGTTTCACTT TGAGGGATGG CAACAAGACC ATTGGCACAG GCCTTGTCAC TGATGTACCA 
GCCATGACCG AGGAGGACAA GAACATCAAA TGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of mitochondria translation elongation factor Tu as a contributor to oxidative damage of postburn myocardium.
Journal of proteomics77469-79(2012 Dec)
Zhang DX,Yan H,Hu JY,Zhang JP,Teng M,Tong DL,Xiang F,Zhang Q,Fang YD,Liang GP,Huang YS

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Chronic ethanol feeding causes depression of mitochondrial elongation factor Tu in the rat liver: implications for the mitochondrial ribosome.
American journal of physiology. Gastrointestinal and liver physiology300(5)G815-22(2011 May)
Weiser B,Gonye G,Sykora P,Crumm S,Cahill A

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W