TUFM cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TUFM
Entrez Gene ID	281556
Full Name	Tu translation elongation factor, mitochondrial
General protein information	Preferred Names Tu translation elongation factor, mitochondrial Names elongation factor Tu, mitochondrial EF-Tu
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174207.2	NP_776632.1	elongation factor Tu, mitochondrial precursor

Pathways from BioSystems

Homologies

	TUFM	XP_001103519.1
Homo sapiens (human)	TUFM	NP_003312.3
Saccharomyces cerevisiae (baker's yeast)	TUF1	NP_014830.1
Kluyveromyces lactis	KLLA0A04587g	XP_451201.1
Kluyveromyces lactis	KLLA0C05214g	XP_452433.1
Eremothecium gossypii	AGOS_AAR143W	NP_982685.1
Magnaporthe oryzae (rice blast fungus)	MGG_08162	XP_003715132.1
Canis lupus familiaris (dog)	TUFM	XP_536924.3
Bos taurus (cattle)	TUFM	NP_776632.1
Rattus norvegicus (Norway rat)	Tufm	NP_001099765.1
Danio rerio (zebrafish)	tufm	NP_001013523.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006996	XP_308774.4
Neurospora crassa	NCU03737	XP_961513.1
Pan troglodytes (chimpanzee)	TUFM	XP_510904.2
Drosophila melanogaster (fruit fly)	EfTuM	NP_001163144.1
Schizosaccharomyces pombe (fission yeast)	tuf1	NP_595746.1
Arabidopsis thaliana (thale cress)	AT4G02930	NP_192202.1
Oryza sativa (rice)	Os03g0851100	NP_001051912.1
Mus musculus (house mouse)	Tufm	NP_766333.1
Caenorhabditis elegans (roundworm)	tufm-1	NP_497623.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.
Akama K, Christian BE, Jones CN, Ueda T, Takeuchi N, Spremulli LL
Biochimica et biophysica acta1802(7-8)692-8(2010 Jul-Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Chaperone properties of mammalian mitochondrial translation elongation factor Tu.
Suzuki H, Ueda T, Taguchi H, Takeuchi N
The Journal of biological chemistry282(6)4076-84(2007 Feb)

Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.
Jeppesen MG, Navratil T, Spremulli LL, Nyborg J
The Journal of biological chemistry280(6)5071-81(2005 Feb)

Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAs.
Hunter SE, Spremulli LL
RNA biology1(2)95-102(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of activity is caused by a significant reduction in the ability of EF-Ts(mt) R325W to bind EF-Tu(mt), leading to a defect in nucleotide exchange.
Title: Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.

Recombinant mitochondrial EF-Tu prevents thermal aggregation of proteins and enhances protein refolding in vitro.
Title: Chaperone properties of mammalian mitochondrial translation elongation factor Tu.

X-ray crystallographic structure of the EF-Tumt.Tsmt complex
Title: Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.

The arginine residue at position 335 of bovine mitochondrial elongation factor Tu (EF-Tu) plays an important role in the binding and EF-Tu-mediated delivery of mitochondrial aminoacyl-tRNAs to the A-site of the ribosome.
Title: Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAs.

Isolation, crystallisation, and preliminary X-ray analysis
Title: Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes.

The following TUFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41643	NM_174207.2 Latest version!	Bos taurus Tu translation elongation factor, mitochondrial (TUFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TUFM ( NM_174207.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776632.1 Bos taurus Tu translation elongation factor, mitochondrial (TUFM), mRNA.

Price & Availability↑

CloneID	OBa41643
Clone ID Related Accession (Same CDS sequence)	NM_174207.2
Accession Version	NM_174207.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	elongation factor Tu, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L38996.1. On Jun 3, 2003 this sequence version replaced NM_174207.1. ##Evidence-Data-START## Transcript exon combination :: L38996.1, BC120109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCCG CTACCCTGCT GCGCGCGACG CCCCTTTTCA GCGGTCTCGG CGCCGGCCCG 
GCACCACTAT TGCAGGGTCT TTTGCGGCCG CTGAAGGCTC AGGCACTGCC CGTCTTGTGC 
CGTGGCCTGG CTGTGGAGGC CAAGAAGACC TATGTGCGTG ACAAGCCCCA TGTGAACGTG 
GGTACCATCG GCCATGTAGA CCATGGCAAG ACCACCCTGA CCGCGGCCAT CACGAAAATT 
TTAGCGGAGG GAGGTGGGGC CAAGTTTAAG AAGTACGAAG AGATTGACAA CGCCCCAGAG 
GAGCGAGCCC GAGGTATCAC AATCAATGCG GCTCATGTGG AGTATAGCAC AGCCGCCCGC 
CACTATGCCC ACACTGACTG CCCGGGTCAT GCAGATTACG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TCGACGGCTG CATCCTGGTG GTGGCAGCCA ATGATGGCCC CATGCCCCAG 
ACCCGAGAAC ACTTACTGCT AGCCAGACAG ATTGGCGTCG AGCATGTGGT GGTGTATGTG 
AACAAGGCGG ACGCTGTGCA GGACTCTGAG ATGGTGGAGC TGGTGGAGCT GGAGATCAGG 
GAACTGCTGA CTGAGTTTGG CTACAAAGGG GAGGAGACTC CGATCATTGT CGGTTCTGCC 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCCTGA AGTCTGTGCA GAAGCTACTG 
GATGCTGTGG ACACATACAT CCCGGTGCCC ACCCGGGACC TGGAGAAGCC TTTTCTGCTG 
CCCGTGGAGT CAGTGTACTC GATCCCCGGC CGGGGCACAG TGGTGACAGG CACGCTCGAA 
CGTGGCATTT TGAAGAAAGG AGACGAGTGT GAATTCCTGG GACACAGCAA GAATATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG ATAGGGCAGA GGCGGGCGAC 
AACCTTGGGG CCCTGGTCCG AGGCTTGAAG CGGGAGGACC TGAGACGTGG CCTGGTCATG 
GCCAAGCCAG GTTCCATCCA GCCTCACCAG AAGGTGGAGG CACAGGTGTA CATCCTCACC 
AAGGAAGAGG GTGGCCGCCA CAAGCCTTTT GTATCCCACT TCATGCCTGT CATGTTCTCC 
CTGACTTGGG ACATGGCCTG TCGGATCATC TTGCCTCCAG GGAAGGAGCT GGCCATGCCC 
GGGGAGGACC TGAAACTCAC TCTGATCTTG CGGCAGCCAA TGATCTTAGA GAAAGGCCAG 
CGTTTCACCC TGCGGGATGG CAACCGGACC ATCGGCACCG GCCTCGTCAC TGACACGCCG 
GCCATGACCG AGGAGGACAA GAACATCAAG TGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776632.1
CDS	67..1425
Translation	MAAATLLRATPLFSGLGAGPAPLLQGLLRPLKAQALPVLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPIIVGSALCALEQRDPELGLKSVQKLLDAVDTYIPVPTRDLEKPFLLPVESVYSIPGRGTVVTGTLERGILKKGDECEFLGHSKNIRTVVTGIEMFHKSLDRAEAGDNLGALVRGLKREDLRRGLVMAKPGSIQPHQKVEAQVYILTKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPGKELAMPGEDLKLTLILRQPMILEKGQRFTLRDGNRTIGTGLVTDTPAMTEEDKNIKWS

Target ORF information:

RefSeq Version	NM_174207.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Tu translation elongation factor, mitochondrial (TUFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174207.2

ORF Insert Sequence:

ATGGCGGCCG CTACCCTGCT GCGCGCGACG CCCCTTTTCA GCGGTCTCGG CGCCGGCCCG 
GCACCACTAT TGCAGGGTCT TTTGCGGCCG CTGAAGGCTC AGGCACTGCC CGTCTTGTGC 
CGTGGCCTGG CTGTGGAGGC CAAGAAGACC TATGTGCGTG ACAAGCCCCA TGTGAACGTG 
GGTACCATCG GCCATGTAGA CCATGGCAAG ACCACCCTGA CCGCGGCCAT CACGAAAATT 
TTAGCGGAGG GAGGTGGGGC CAAGTTTAAG AAGTACGAAG AGATTGACAA CGCCCCAGAG 
GAGCGAGCCC GAGGTATCAC AATCAATGCG GCTCATGTGG AGTATAGCAC AGCCGCCCGC 
CACTATGCCC ACACTGACTG CCCGGGTCAT GCAGATTACG TTAAGAATAT GATCACAGGC 
ACTGCCCCCC TCGACGGCTG CATCCTGGTG GTGGCAGCCA ATGATGGCCC CATGCCCCAG 
ACCCGAGAAC ACTTACTGCT AGCCAGACAG ATTGGCGTCG AGCATGTGGT GGTGTATGTG 
AACAAGGCGG ACGCTGTGCA GGACTCTGAG ATGGTGGAGC TGGTGGAGCT GGAGATCAGG 
GAACTGCTGA CTGAGTTTGG CTACAAAGGG GAGGAGACTC CGATCATTGT CGGTTCTGCC 
CTCTGTGCCC TTGAGCAACG TGACCCTGAG CTAGGCCTGA AGTCTGTGCA GAAGCTACTG 
GATGCTGTGG ACACATACAT CCCGGTGCCC ACCCGGGACC TGGAGAAGCC TTTTCTGCTG 
CCCGTGGAGT CAGTGTACTC GATCCCCGGC CGGGGCACAG TGGTGACAGG CACGCTCGAA 
CGTGGCATTT TGAAGAAAGG AGACGAGTGT GAATTCCTGG GACACAGCAA GAATATTCGC 
ACTGTGGTGA CAGGCATTGA GATGTTCCAC AAGAGCCTGG ATAGGGCAGA GGCGGGCGAC 
AACCTTGGGG CCCTGGTCCG AGGCTTGAAG CGGGAGGACC TGAGACGTGG CCTGGTCATG 
GCCAAGCCAG GTTCCATCCA GCCTCACCAG AAGGTGGAGG CACAGGTGTA CATCCTCACC 
AAGGAAGAGG GTGGCCGCCA CAAGCCTTTT GTATCCCACT TCATGCCTGT CATGTTCTCC 
CTGACTTGGG ACATGGCCTG TCGGATCATC TTGCCTCCAG GGAAGGAGCT GGCCATGCCC 
GGGGAGGACC TGAAACTCAC TCTGATCTTG CGGCAGCCAA TGATCTTAGA GAAAGGCCAG 
CGTTTCACCC TGCGGGATGG CAACCGGACC ATCGGCACCG GCCTCGTCAC TGACACGCCG 
GCCATGACCG AGGAGGACAA GAACATCAAG TGGAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.
Biochimica et biophysica acta1802(7-8)692-8(2010 Jul-Aug)
Akama K,Christian BE,Jones CN,Ueda T,Takeuchi N,Spremulli LL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Chaperone properties of mammalian mitochondrial translation elongation factor Tu.
The Journal of biological chemistry282(6)4076-84(2007 Feb)
Suzuki H,Ueda T,Taguchi H,Takeuchi N

Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.
The Journal of biological chemistry280(6)5071-81(2005 Feb)
Jeppesen MG,Navratil T,Spremulli LL,Nyborg J

Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAs.
RNA biology1(2)95-102(2004 Jul)
Hunter SE,Spremulli LL