Tac3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tac3
Entrez Gene ID	29191
Full Name	tachykinin 3
Synonyms	Tac2
General protein information	Preferred Names tachykinin 3 Names tachykinin-3 neurokinin B tachykinin 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	putative tachykinin protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019162.2	NP_062035.1	tachykinin-3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TAC3	XP_001168338.1
	TAC3	XP_001115535.1
Rattus norvegicus (Norway rat)	Tac3	NP_062035.1
Bos taurus (cattle)	TAC3	NP_851360.1
Canis lupus familiaris (dog)	TAC3	XP_005625588.1
Homo sapiens (human)	TAC3	NP_037383.1
Mus musculus (house mouse)	Tac2	NP_033338.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of hypothalamic kisspeptin, neurokinin B, and dynorphin A neurons attenuates in female Zucker fatty rats.
Nakao K, Iwata K, Takeshita T, Ozawa H
Neuroscience letters665135-139(2017 Dec)

Effects and interactions of tachykinins and dynorphin on FSH and LH secretion in developing and adult rats.
Ruiz-Pino F, Garcia-Galiano D, Manfredi-Lozano M, Leon S, S?nchez-Garrido MA, Roa J, Pinilla L, Navarro VM, Tena-Sempere M
Endocrinology156(2)576-88(2015 Feb)

Disparate changes in kisspeptin and neurokinin B expression in the arcuate nucleus after sex steroid manipulation reveal differential regulation of the two KNDy peptides in rats.
Overgaard A, Ruiz-Pino F, Castellano JM, Tena-Sempere M, Mikkelsen JD
Endocrinology155(10)3945-55(2014 Oct)

Neurokinin B receptor antagonism decreases luteinising hormone pulse frequency and amplitude and delays puberty onset in the female rat.
Li SY, Li XF, Hu MH, Shao B, Poston L, Lightman SL, O'Byrne KT
Journal of neuroendocrinology26(8)521-7(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NKB agonist stimulates FSH secretion in a strikingly developmental-dependent manner
Title: Effects and interactions of tachykinins and dynorphin on FSH and LH secretion in developing and adult rats.

The divergent regulation of kisspeptin and NKB peptide contents in the arcuate nucleus as a function of sex and steroid milieu enlarge our understanding on how these neuropeptides are posttranscriptionally regulated in KNDy neurons.
Title: Disparate changes in kisspeptin and neurokinin B expression in the arcuate nucleus after sex steroid manipulation reveal differential regulation of the two KNDy peptides in rats.

data suggest that endogenous NeuroKinin B-Neurokinin 3 Receptor signalling plays a role in controlling the timing of puberty.
Title: Neurokinin B receptor antagonism decreases luteinising hormone pulse frequency and amplitude and delays puberty onset in the female rat.

Data indicate that the suppression of the GnRH pulse generator by acute systemic stress equires hypothalamic NKB/neurokinin B (NKB) receptor (NK3R) signaling.
Title: Neurokinin B signaling in the female rat: a novel link between stress and reproduction.

Data suggest few hypothalamic genes are sexually dimorphic during postnatal development; only Tac2/neurokinin B, Esr1 (estrogen receptor 1), and Kiss1 (KiSS-1 metastasis-suppressor) are dimorphic (i.e., higher in females) in developing preoptic area.
Title: Molecular profiling of postnatal development of the hypothalamus in female and male rats.

The following Tac3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tac3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03906	NM_019162.2 Latest version!	Rattus norvegicus tachykinin precursor 3 (Tac3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tac3 ( NM_019162.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062035.1 Rattus norvegicus tachykinin precursor 3 (Tac3), mRNA.

Price & Availability↑

CloneID	ORa03906
Clone ID Related Accession (Same CDS sequence)	NM_019162.2
Accession Version	NM_019162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	tachykinin-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M16410.1. On Feb 21, 2013 this sequence version replaced NM_019162.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M16410.1, DV713681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAGCG CCATGCTGTT CGCGGCTGTC CTCGCCCTCA GCTTGGCATG GACCTTCGGG 
GCTGCGTGTG AGGAACCTCA GGAGCAGGGT GGGAGGCTCA GTAAGGACTC TGATCTCTCT 
CTGCTACCTC CACCCCTGCT TCGGAGACTC TACGACAGCC GCTCCATCTC TCTGGAAGGA 
TTGCTGAAAG TGCTGAGCAA GGCTAGCGTG GGACCGAAGG AGACATCACT TCCACAGAAA 
CGTGACATGC ACGACTTCTT TGTGGGACTT ATGGGCAAGA GGAACAGCCA ACCAGACACT 
CCCGCTGATG TGGTTGAAGA GAACACCCCC AGCTTTGGCG TCCTCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062035.1
CDS	115..465
Translation	MRSAMLFAAVLALSLAWTFGAACEEPQEQGGRLSKDSDLSLLPPPLLRRLYDSRSISLEGLLKVLSKASVGPKETSLPQKRDMHDFFVGLMGKRNSQPDTPADVVEENTPSFGVLK

Target ORF information:

RefSeq Version	NM_019162.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tachykinin precursor 3 (Tac3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019162.2

ORF Insert Sequence:

ATGAGGAGCG CCATGCTGTT CGCGGCTGTC CTCGCCCTCA GCTTGGCATG GACCTTCGGG 
GCTGCGTGTG AGGAACCTCA GGAGCAGGGT GGGAGGCTCA GTAAGGACTC TGATCTCTCT 
CTGCTACCTC CACCCCTGCT TCGGAGACTC TACGACAGCC GCTCCATCTC TCTGGAAGGA 
TTGCTGAAAG TGCTGAGCAA GGCTAGCGTG GGACCGAAGG AGACATCACT TCCACAGAAA 
CGTGACATGC ACGACTTCTT TGTGGGACTT ATGGGCAAGA GGAACAGCCA ACCAGACACT 
CCCGCTGATG TGGTTGAAGA GAACACCCCC AGCTTTGGCG TCCTCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of hypothalamic kisspeptin, neurokinin B, and dynorphin A neurons attenuates in female Zucker fatty rats.
Neuroscience letters665135-139(2017 Dec)
Nakao K,Iwata K,Takeshita T,Ozawa H

Effects and interactions of tachykinins and dynorphin on FSH and LH secretion in developing and adult rats.
Endocrinology156(2)576-88(2015 Feb)
Ruiz-Pino F,Garcia-Galiano D,Manfredi-Lozano M,Leon S,S?nchez-Garrido MA,Roa J,Pinilla L,Navarro VM,Tena-Sempere M

Disparate changes in kisspeptin and neurokinin B expression in the arcuate nucleus after sex steroid manipulation reveal differential regulation of the two KNDy peptides in rats.
Endocrinology155(10)3945-55(2014 Oct)
Overgaard A,Ruiz-Pino F,Castellano JM,Tena-Sempere M,Mikkelsen JD

Neurokinin B receptor antagonism decreases luteinising hormone pulse frequency and amplitude and delays puberty onset in the female rat.
Journal of neuroendocrinology26(8)521-7(2014 Aug)
Li SY,Li XF,Hu MH,Shao B,Poston L,Lightman SL,O'Byrne KT