Shpk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shpk
Entrez Gene ID	287479
Full Name	sedoheptulokinase
Synonyms	Carkl
General protein information	Preferred Names sedoheptulokinase Names sedoheptulokinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001033682.1	NP_001028854.1	sedoheptulokinase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SHPK	XP_548339.1
Gallus gallus (chicken)	SHPK	XP_415852.4
Xenopus tropicalis (tropical clawed frog)	shpk	NP_001120078.1
Homo sapiens (human)	SHPK	NP_037408.2
Mus musculus (house mouse)	Shpk	NP_083307.1
	LOC721465	XP_001117628.1
Rattus norvegicus (Norway rat)	Shpk	NP_001028854.1
Pan troglodytes (chimpanzee)	SHPK	XP_523551.3
Bos taurus (cattle)	SHPK	NP_001069016.1
Danio rerio (zebrafish)	shpk	XP_001341627.4
Caenorhabditis elegans (roundworm)	CELE_T25C8.1	NP_499810.2

Gene Ontology
Bibliography

Related articles in PubMed

The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism.
Haschemi A, Kosma P, Gille L, Evans CR, Burant CF, Starkl P, Knapp B, Haas R, Schmid JA, Jandl C, Amir S, Lubec G, Park J, Esterbauer H, Bilban M, Brizuela L, Pospisilik JA, Otterbein LE, Wagner O
Cell metabolism15(6)813-26(2012 Jun)

Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S
Analytical chemistry81(11)4493-501(2009 Jun)

Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency.
Kardon T, Stroobant V, Veiga-da-Cunha M, Schaftingen EV
FEBS letters582(23-24)3330-4(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Shpk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shpk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08551	NM_001033682.1 Latest version!	Rattus norvegicus sedoheptulokinase (Shpk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shpk ( NM_001033682.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001028854.1 Rattus norvegicus sedoheptulokinase (Shpk), mRNA.

Price & Availability↑

CloneID	ORa08551
Clone ID Related Accession (Same CDS sequence)	NM_001033682.1
Accession Version	NM_001033682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	sedoheptulokinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC101914.1. On Sep 25, 2005 this sequence version replaced XM_220650.3. ##Evidence-Data-START## Transcript exon combination :: BC101914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACTTCGC GACCCGTCAC TCTCGGTATC GACTTGGGCA CCACGTCCGT AAAGGCCGCC 
CTCTTGGAGG CTGCACCCGG CCACCCATCC GGGTTCGTGG TGCTGGCGAG CTGCGCCCGG 
GCTGCGGGGG CAGAGACCGA GAGCGCGGCT GCCGGACCCC AGGGGCGGGA GCAGGATGTG 
ACTAGAATCA TCCAAGCTCT AAATGAATGC CTTGATGCCC TTCCCCCGAG GCAGCTCCAG 
AGAGTCGGGG CCATCGGAGT GTCAGGACAG ATGCATGGCA TCCTATTTTG GAAAACAGGC 
CAAGGCTGTG AATGGACAGA GAGAGGGTCT GCCCCTGTAT TTGAACCCAG AGCCGTGAGC 
CATTTGGTCA CATGGCAGGA TAACCGTTGT AACAGTGGTT TCCTGGCTTC TCTGCCCAAG 
CCAGCATCCC ATCTCAGCGT GGCCACAGGA TTCGGCTGTG CAACCATCTT CTGGCTTTTG 
AAAAACAGCC CGGAGTTTCT GAAGTCCTAT GACGTGGCTG GCACCATTCA AGACTATGTG 
GTTGCTATGC TGTGTGGCTT GCCAAGACCC CTGATGTCTG ACCAGAACGC TGCTAGCTGG 
GGCTATTTTA ACACCCAGAG CCAGAGCTGG AACTCGGACA TACTGCAAAT GGCAGGTTTT 
CCTACCCACC TTCTCCCCGA CATTGCAGAG CCAGGCAGCT TGGCAGGCAG AACTTCTCAC 
ACGTGGTTTG AAATCCCAGC AGGGACGGAG GTGGGCGTAG CCCTGGGTGA CTTACAAGCC 
TCTGTCTATT CCTGCATGGG CCAAAGGACT GATGCAGTTC TCAACATCAG CACCTCGGTC 
CAGCTGGCAG CCTCCATGCC CGTGGGGTTC CAGCCACCGC AGACTCCGGA TCCTGCAGCC 
CCAGTCGCCT TCTTCCCTTA CTTCGACAGG ACCTACCTAG GCGTGGCAGC GTCACTCAAC 
GGAGGCAATG TGCTGGCCAC GTTTGTCCAC ATGCTTGTTC AGTGGATGAC AGACCTAGGT 
CTGGAGGTTG AAGAATCCAC TGTCTATTCA CGCATGATCC AGGCGGCTGC ACAGCAGAAA 
GATACCCATC TCACCATCAC CCCAACAGTG CTGGGGGAGA GGCACCTGCC AGACCAGCTG 
GCCTCTGTGA CTAGAATCTC CTCCTCTGAC CTTTCCCTGG GCCATGTGAC CCGGGCCCTA 
TGCAGAGGCA TTGTTCAGAA CCTGCACTCC ATGCTTCCTT TTCAACAGCT CCAAGAGTGG 
GGTGTGGAAC GGGTGGTGGG GAGTGGAAGT GCATTGTCCA GGAATGAGGT CCTGAAGCAG 
GAGGTCCAGA GGGCATTTCC TTTCCCAGTG TGCTTTGGGC AGGATGTAGA TGCAGCCTTC 
GGGGCAGCTT TGGTCATGCT CCGGAGAGAC ATCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028854.1
CDS	30..1448
Translation	MTSRPVTLGIDLGTTSVKAALLEAAPGHPSGFVVLASCARAAGAETESAAAGPQGREQDVTRIIQALNECLDALPPRQLQRVGAIGVSGQMHGILFWKTGQGCEWTERGSAPVFEPRAVSHLVTWQDNRCNSGFLASLPKPASHLSVATGFGCATIFWLLKNSPEFLKSYDVAGTIQDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNSDILQMAGFPTHLLPDIAEPGSLAGRTSHTWFEIPAGTEVGVALGDLQASVYSCMGQRTDAVLNISTSVQLAASMPVGFQPPQTPDPAAPVAFFPYFDRTYLGVAASLNGGNVLATFVHMLVQWMTDLGLEVEESTVYSRMIQAAAQQKDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPFQQLQEWGVERVVGSGSALSRNEVLKQEVQRAFPFPVCFGQDVDAAFGAALVMLRRDIS

Target ORF information:

RefSeq Version	NM_001033682.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sedoheptulokinase (Shpk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033682.1

ORF Insert Sequence:

ATGACTTCGC GACCCGTCAC TCTCGGTATC GACTTGGGCA CCACGTCCGT AAAGGCCGCC 
CTCTTGGAGG CTGCACCCGG CCACCCATCC GGGTTCGTGG TGCTGGCGAG CTGCGCCCGG 
GCTGCGGGGG CAGAGACCGA GAGCGCGGCT GCCGGACCCC AGGGGCGGGA GCAGGATGTG 
ACTAGAATCA TCCAAGCTCT AAATGAATGC CTTGATGCCC TTCCCCCGAG GCAGCTCCAG 
AGAGTCGGGG CCATCGGAGT GTCAGGACAG ATGCATGGCA TCCTATTTTG GAAAACAGGC 
CAAGGCTGTG AATGGACAGA GAGAGGGTCT GCCCCTGTAT TTGAACCCAG AGCCGTGAGC 
CATTTGGTCA CATGGCAGGA TAACCGTTGT AACAGTGGTT TCCTGGCTTC TCTGCCCAAG 
CCAGCATCCC ATCTCAGCGT GGCCACAGGA TTCGGCTGTG CAACCATCTT CTGGCTTTTG 
AAAAACAGCC CGGAGTTTCT GAAGTCCTAT GACGTGGCTG GCACCATTCA AGACTATGTG 
GTTGCTATGC TGTGTGGCTT GCCAAGACCC CTGATGTCTG ACCAGAACGC TGCTAGCTGG 
GGCTATTTTA ACACCCAGAG CCAGAGCTGG AACTCGGACA TACTGCAAAT GGCAGGTTTT 
CCTACCCACC TTCTCCCCGA CATTGCAGAG CCAGGCAGCT TGGCAGGCAG AACTTCTCAC 
ACGTGGTTTG AAATCCCAGC AGGGACGGAG GTGGGCGTAG CCCTGGGTGA CTTACAAGCC 
TCTGTCTATT CCTGCATGGG CCAAAGGACT GATGCAGTTC TCAACATCAG CACCTCGGTC 
CAGCTGGCAG CCTCCATGCC CGTGGGGTTC CAGCCACCGC AGACTCCGGA TCCTGCAGCC 
CCAGTCGCCT TCTTCCCTTA CTTCGACAGG ACCTACCTAG GCGTGGCAGC GTCACTCAAC 
GGAGGCAATG TGCTGGCCAC GTTTGTCCAC ATGCTTGTTC AGTGGATGAC AGACCTAGGT 
CTGGAGGTTG AAGAATCCAC TGTCTATTCA CGCATGATCC AGGCGGCTGC ACAGCAGAAA 
GATACCCATC TCACCATCAC CCCAACAGTG CTGGGGGAGA GGCACCTGCC AGACCAGCTG 
GCCTCTGTGA CTAGAATCTC CTCCTCTGAC CTTTCCCTGG GCCATGTGAC CCGGGCCCTA 
TGCAGAGGCA TTGTTCAGAA CCTGCACTCC ATGCTTCCTT TTCAACAGCT CCAAGAGTGG 
GGTGTGGAAC GGGTGGTGGG GAGTGGAAGT GCATTGTCCA GGAATGAGGT CCTGAAGCAG 
GAGGTCCAGA GGGCATTTCC TTTCCCAGTG TGCTTTGGGC AGGATGTAGA TGCAGCCTTC 
GGGGCAGCTT TGGTCATGCT CCGGAGAGAC ATCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism.
Cell metabolism15(6)813-26(2012 Jun)
Haschemi A,Kosma P,Gille L,Evans CR,Burant CF,Starkl P,Knapp B,Haas R,Schmid JA,Jandl C,Amir S,Lubec G,Park J,Esterbauer H,Bilban M,Brizuela L,Pospisilik JA,Otterbein LE,Wagner O

Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
Analytical chemistry81(11)4493-501(2009 Jun)
Gauci S,Helbig AO,Slijper M,Krijgsveld J,Heck AJ,Mohammed S

Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency.
FEBS letters582(23-24)3330-4(2008 Oct)
Kardon T,Stroobant V,Veiga-da-Cunha M,Schaftingen EV