Slc1a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc1a1
Entrez Gene ID	25550
Full Name	solute carrier family 1 member 1
Synonyms	Eaac1,Eaat3,REAAC1
General protein information	Preferred Names solute carrier family 1 member 1 Names excitatory amino acid transporter 3 Solute carrier family 1 A1 (brain glutamate transporter) excitatory amino-acid carrier 1 sodium-dependent glutamate/aspartate transporter 3 solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q52
Summary	mediates sodium ion dependent high affinity glutamate transport and proton cotransport; plays a role in maintenance of neuronal function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013032.3	NP_037164.3	excitatory amino acid transporter 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC1A1	NP_004161.4
Canis lupus familiaris (dog)	SLC1A1	NP_001003139.1
Rattus norvegicus (Norway rat)	Slc1a1	NP_037164.3
Danio rerio (zebrafish)	slc1a1	NP_001002666.2
Pan troglodytes (chimpanzee)	SLC1A1	XP_520467.2
	SLC1A1	XP_001085339.1
Mus musculus (house mouse)	Slc1a1	NP_033225.1
Gallus gallus (chicken)	SLC1A1	XP_424930.4
Xenopus tropicalis (tropical clawed frog)	slc1a1	XP_002934228.2
Bos taurus (cattle)	SLC1A1	NP_777024.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006718	XP_309025.4

Gene Ontology
Bibliography

Related articles in PubMed

The cystine-glutamate exchanger (xCT, Slc7a11) is expressed in significant concentrations in a subpopulation of astrocytes in the mouse brain.
Ottestad-Hansen S, Hu QX, Follin-Arbelet VV, Bentea E, Sato H, Massie A, Zhou Y, Danbolt NC
Glia(2018 Jan)

MicroRNA 101b Is Downregulated in the Prefrontal Cortex of a Genetic Model of Depression and Targets the Glutamate Transporter SLC1A1 (EAAT3) in Vitro.
Wei YB, Melas PA, Villaescusa JC, Liu JJ, Xu N, Christiansen SH, Elbr?nd-Bek H, Woldbye DP, Wegener G, Math? AA, Lavebratt C
The international journal of neuropsychopharmacology19(12)(2016 Dec)

Numb directs the subcellular localization of EAAT3 through binding the YxNxxF motif.
Su JF, Wei J, Li PS, Miao HH, Ma YC, Qu YX, Xu J, Qin J, Li BL, Song BL, Xu ZP, Luo J
Journal of cell science129(16)3104-14(2016 08)

Caveolin-1 Sensitivity of Excitatory Amino Acid Transporters EAAT1, EAAT2, EAAT3, and EAAT4.
Abousaab A, Warsi J, Elvira B, Lang F
The Journal of membrane biology249(3)239-49(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-101b targets the neuronal glutamate transporter SLC1A1. Both mRNA and protein levels of SLC1A1 were found to be upregulated in the prefrontal cortex of rats genetically predisposed to develop major depressive disorder.
Title: MicroRNA 101b Is Downregulated in the Prefrontal Cortex of a Genetic Model of Depression and Targets the Glutamate Transporter SLC1A1 (EAAT3) in Vitro.

Numb is a pivotal adaptor protein that mediates the subcellular localization of EAAT3 through binding the YxNxxF (where x stands for any amino acid) motif.
Title: Numb directs the subcellular localization of EAAT3 through binding the YxNxxF motif.

Exercise-Mediated Increase in Nigral EAAC1 in aging rats.
Title: Exercise-Mediated Increase in Nigral Tyrosine Hydroxylase Is Accompanied by Increased Nigral GFR-?1 and EAAC1 Expression in Aging Rats.

data indicate that NRG1 induced the up-regulation of EAAC1 in primary cortical neurons with an increase in glutamate uptake
Title: Neuregulin 1 Controls Glutamate Uptake by Up-regulating Excitatory Amino Acid Carrier 1 (EAAC1).

Chronic postnatal stress led to decreased Eaat3 expression in hippocampus and frontal cortex.
Title: Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats.

The following Slc1a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc1a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12694	NM_013032.3 Latest version!	Rattus norvegicus solute carrier family 1 member 1 (Slc1a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc1a1 ( NM_013032.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037164.3 Rattus norvegicus solute carrier family 1 member 1 (Slc1a1), mRNA.

Price & Availability↑

CloneID	ORa12694
Clone ID Related Accession (Same CDS sequence)	NM_013032.3
Accession Version	NM_013032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	excitatory amino acid transporter 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061743.1. On Jun 10, 2007 this sequence version replaced NM_013032.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061743.1, U39555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAAGC CCACGAGCTC GGGATGCGAC TGGCGCCGCT TCCTGCGGAA TCACTGGCTG 
CTGCTCTCCA CCGTGGCCGC CGTGGTACTG GGCATCGTGG TAGGAGTCTT GGTTCGAGGA 
CACAGTGAGC TCTCGAATCT GGATAAATTC TACTTTGCGT TTCCTGGAGA AATTCTGATG 
CGGATGCTGA AGCTCGTCAT TCTGCCACTG ATTATATCCA GCATGATCAC AGGTGTCGCT 
GCACTGGATT CCAACGTATC TGGAAAAATT GGTCTTCGTG CTGTAGTATA TTATTTCTCC 
ACCACCGTCA TTGCTGTAAT CCTAGGTATT GTGTTAGTTG TGAGCATCAA GCCTGGTGTC 
ACTCAGAAAG TGAATGAAAT CAACAGGACA GGCAAAACCC CTGAAGTCAG CACTGTGGAC 
GCCATGTTGG ATCTGATCAG GAACATGTTC CCTGAGAACC TGGTCCAAGC CTGTTTTCAG 
CAGTACAAAA CCAAGCGTGA AGAAGTGAAG CCTGCCAGTG ATCCTGGGGG GAACCAGACA 
GAGGTGTCTG TCACCACGGC CATGACAACA ATGTCTGAGA ACAAGACAAA GGAATACAAA 
ATCGTGGGCC TGTACTCAGA CGGCATCAAC GTCCTGGGCC TGATTATCTT CTGCCTCGTC 
TTTGGACTTG TCATTGGAAA AATGGGAGAA AAAGGGCAGA TTCTGGTGGA TTTCTTCAAT 
GCGTTGAGTG ACGCCACCAT GAAAATCGTT CAGATCATCA TGTGTTACAT GCCGATCGGT 
ATTTTGTTCC TAATCGCTGG GAAGATCATA GAAGTTGAAG ACTGGGAAAT ATTCCGCAAG 
TTGGGCCTTT ATATGGCCAC TGTCCTGAGT GGGCTTGCAA TCCACTCCCT CGTAGTGCTG 
CCACTGATCT ATTTCATAGT CGTGCGGAAG AACCCTTTCC GCTTTGCCTT GGGCATGGCG 
CAGGCTCTCC TGACAGCTCT CATGATCTCA TCTAGCTCGG CAACCCTTCC AGTTACATTC 
CGCTGTGCGG AAGAAAAGAA CCACGTGGAC AAGAGGATCA CAAGATTTGT GCTGCCCGTC 
GGCGCCACCA TCAACATGGA CGGCACTGCG CTCTATGAAG CCGTGGCAGC TGTGTTCATT 
GCGCAGCTGA ACGGCATGGA CCTGAGCATT GGGCAGATCA TCACGATCAG CATCACAGCC 
ACCGCTGCTA GCATTGGAGC TGCCGGTGTG CCCCAGGCTG GCCTGGTGAC CATGGTGATC 
GTGTTGAGTG CCGTGGGGCT GCCTGCTGAG GACGTCACCC TCATCATTGC CGTCGACTGG 
CTCCTGGACC GGTTCAGGAC CATGGTGAAC GTTCTGGGTG ATGCGTTTGG GACGGGCATC 
GTGGAGAAGC TGTCCAAGAA GGAGCTGGAG CAGGTGGATG TCTCCTCTGA AGTCAACATT 
GTGAACCCCT TTGCCTTGGA GCCCACAATC CTGGATAATG AAGACTCAGA TACGAAGAAG 
TCTTACGTCA ATGGGGGCTT CTCGGTAGAC AAATCTGACA CCATCTCGTT CACTCAGACC 
TCGCAGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037164.3
CDS	90..1661
Translation	MGKPTSSGCDWRRFLRNHWLLLSTVAAVVLGIVVGVLVRGHSELSNLDKFYFAFPGEILMRMLKLVILPLIISSMITGVAALDSNVSGKIGLRAVVYYFSTTVIAVILGIVLVVSIKPGVTQKVNEINRTGKTPEVSTVDAMLDLIRNMFPENLVQACFQQYKTKREEVKPASDPGGNQTEVSVTTAMTTMSENKTKEYKIVGLYSDGINVLGLIIFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPIGILFLIAGKIIEVEDWEIFRKLGLYMATVLSGLAIHSLVVLPLIYFIVVRKNPFRFALGMAQALLTALMISSSSATLPVTFRCAEEKNHVDKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNGMDLSIGQIITISITATAASIGAAGVPQAGLVTMVIVLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQVDVSSEVNIVNPFALEPTILDNEDSDTKKSYVNGGFSVDKSDTISFTQTSQF

Target ORF information:

RefSeq Version	NM_013032.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 1 member 1 (Slc1a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013032.3

ORF Insert Sequence:

ATGGGGAAGC CCACGAGCTC GGGATGCGAC TGGCGCCGCT TCCTGCGGAA TCACTGGCTG 
CTGCTCTCCA CCGTGGCCGC CGTGGTACTG GGCATCGTGG TAGGAGTCTT GGTTCGAGGA 
CACAGTGAGC TCTCGAATCT GGATAAATTC TACTTTGCGT TTCCTGGAGA AATTCTGATG 
CGGATGCTGA AGCTCGTCAT TCTGCCACTG ATTATATCCA GCATGATCAC AGGTGTCGCT 
GCACTGGATT CCAACGTATC TGGAAAAATT GGTCTTCGTG CTGTAGTATA TTATTTCTCC 
ACCACCGTCA TTGCTGTAAT CCTAGGTATT GTGTTAGTTG TGAGCATCAA GCCTGGTGTC 
ACTCAGAAAG TGAATGAAAT CAACAGGACA GGCAAAACCC CTGAAGTCAG CACTGTGGAC 
GCCATGTTGG ATCTGATCAG GAACATGTTC CCTGAGAACC TGGTCCAAGC CTGTTTTCAG 
CAGTACAAAA CCAAGCGTGA AGAAGTGAAG CCTGCCAGTG ATCCTGGGGG GAACCAGACA 
GAGGTGTCTG TCACCACGGC CATGACAACA ATGTCTGAGA ACAAGACAAA GGAATACAAA 
ATCGTGGGCC TGTACTCAGA CGGCATCAAC GTCCTGGGCC TGATTATCTT CTGCCTCGTC 
TTTGGACTTG TCATTGGAAA AATGGGAGAA AAAGGGCAGA TTCTGGTGGA TTTCTTCAAT 
GCGTTGAGTG ACGCCACCAT GAAAATCGTT CAGATCATCA TGTGTTACAT GCCGATCGGT 
ATTTTGTTCC TAATCGCTGG GAAGATCATA GAAGTTGAAG ACTGGGAAAT ATTCCGCAAG 
TTGGGCCTTT ATATGGCCAC TGTCCTGAGT GGGCTTGCAA TCCACTCCCT CGTAGTGCTG 
CCACTGATCT ATTTCATAGT CGTGCGGAAG AACCCTTTCC GCTTTGCCTT GGGCATGGCG 
CAGGCTCTCC TGACAGCTCT CATGATCTCA TCTAGCTCGG CAACCCTTCC AGTTACATTC 
CGCTGTGCGG AAGAAAAGAA CCACGTGGAC AAGAGGATCA CAAGATTTGT GCTGCCCGTC 
GGCGCCACCA TCAACATGGA CGGCACTGCG CTCTATGAAG CCGTGGCAGC TGTGTTCATT 
GCGCAGCTGA ACGGCATGGA CCTGAGCATT GGGCAGATCA TCACGATCAG CATCACAGCC 
ACCGCTGCTA GCATTGGAGC TGCCGGTGTG CCCCAGGCTG GCCTGGTGAC CATGGTGATC 
GTGTTGAGTG CCGTGGGGCT GCCTGCTGAG GACGTCACCC TCATCATTGC CGTCGACTGG 
CTCCTGGACC GGTTCAGGAC CATGGTGAAC GTTCTGGGTG ATGCGTTTGG GACGGGCATC 
GTGGAGAAGC TGTCCAAGAA GGAGCTGGAG CAGGTGGATG TCTCCTCTGA AGTCAACATT 
GTGAACCCCT TTGCCTTGGA GCCCACAATC CTGGATAATG AAGACTCAGA TACGAAGAAG 
TCTTACGTCA ATGGGGGCTT CTCGGTAGAC AAATCTGACA CCATCTCGTT CACTCAGACC 
TCGCAGTTCT AG