Gadd45a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gadd45a
Entrez Gene ID	25112
Full Name	growth arrest and DNA-damage-inducible, alpha
Synonyms	Ddit1,Gadd45
General protein information	Preferred Names growth arrest and DNA-damage-inducible, alpha Names growth arrest and DNA damage-inducible protein GADD45 alpha DDIT-1 DNA damage-inducible transcript 1 protein DNA-damage-inducible transcript 1 growth arrest and DNA-damage-inducible 45 alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	may mediate a delay in G2 to M cell cycle progression; may induce DNA repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024127.2	NP_077041.2	growth arrest and DNA damage-inducible protein GADD45 alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	GADD45A	NP_001915.1
Pan troglodytes (chimpanzee)	GADD45A	XP_513476.2
	GADD45A	XP_001095413.1
Bos taurus (cattle)	GADD45A	NP_001029419.1
Mus musculus (house mouse)	Gadd45a	NP_031862.1
Danio rerio (zebrafish)	gadd45ab	NP_001002216.1
Danio rerio (zebrafish)	gadd45aa	NP_956870.1
Gallus gallus (chicken)	GADD45A	NP_001038143.1
Canis lupus familiaris (dog)	GADD45A	XP_003639149.1
Rattus norvegicus (Norway rat)	Gadd45a	NP_077041.2
Xenopus tropicalis (tropical clawed frog)	gadd45a	NP_001016151.1

Gene Ontology
Bibliography

Related articles in PubMed

GADD45a Promotes Active DNA Demethylation of the MMP-9 Promoter via Base Excision Repair Pathway in AGEs-treated keratinocytes and in diabetic male rat skin.
Zhou L, Wang W, Yang C, Zeng T, Hu M, Wang X, Li N, Sun K, Wang C, Zhou J, Ren M, Yan L
Endocrinology(2017 Dec)

Growth Arrest and DNA-damage-inducible Protein 45?-mediated DNA Demethylation of Voltage-dependent T-type Calcium Channel 3.2 Subunit Enhances Neuropathic Allodynia after Nerve Injury in Rats.
Lai CY, Hsieh MC, Ho YC, Lee AS, Wang HH, Cheng JK, Chau YP, Peng HY
Anesthesiology126(6)1077-1095(2017 06)

Sublytic C5b-9 triggers glomerular mesangial cell apoptosis in rat Thy-1 nephritis via Gadd45 activation mediated by Egr-1 and p300-dependent ATF3 acetylation.
He F, Zhou M, Yu T, Zhao D, Zhang J, Qiu W, Lu Y, Liu Y, Wang L, Wang Y
Journal of molecular cell biology8(6)477-491(2016 Dec)

Sex differences in Gadd45b expression and methylation in the developing rodent amygdala.
Kigar SL, Chang L, Hayne MR, Karls NT, Auger AP
Brain research1642461-466(2016 07)

Limited GADD45? expression and function in IL-1? toxicity towards insulin-producing cells.
Skalniak L, Gurgul-Convey E, Okreglicka K, Skalniak A, Jura J
Acta biochimica Polonica60(4)595-602(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Growth arrest and DNA-damage-inducible protein 45 (Gadd45) is known to relieve methylation-induced gene silencing by promoting DNA demethylation.
Title: Growth Arrest and DNA-damage-inducible Protein 45?-mediated DNA Demethylation of Voltage-dependent T-type Calcium Channel 3.2 Subunit Enhances Neuropathic Allodynia after Nerve Injury in Rats.

sublytic C5b-9-induced activation of Egr-1/p300-ATF3/Gadd45 axis plays a critical role in glomerular mesangial cells apoptosis in Thy-1N rats.
Title: Sublytic C5b-9 triggers glomerular mesangial cell apoptosis in rat Thy-1 nephritis via Gadd45 activation mediated by Egr-1 and p300-dependent ATF3 acetylation.

Report of sex differences in the expression of growth arrest and DNA damage inducible factor beta (Gadd45b), but not family members alpha (a) or gamma (g), in the neonatal and juvenile rodent amygdala
Title: Sex differences in Gadd45b expression and methylation in the developing rodent amygdala.

These findings suggest that the potential engagement of GADD45a in IL-1b toxicity towards beta cells is limited to the effects induced by the basal expression level of this protein.
Title: Limited GADD45? expression and function in IL-1? toxicity towards insulin-producing cells.

reduced Gadd45alpha protein expression by forced miR-499 expression indicated it's a diabetes-associated gene which might potentially be involved in both DCM and DM-induced baroreflex dysfunction
Title: Gadd45?: a novel diabetes-associated gene potentially linking diabetic cardiomyopathy and baroreflex dysfunction.

The following Gadd45a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gadd45a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06773	NM_024127.2 Latest version!	Rattus norvegicus growth arrest and DNA-damage-inducible, alpha (Gadd45a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gadd45a ( NM_024127.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077041.2 Rattus norvegicus growth arrest and DNA-damage-inducible, alpha (Gadd45a), mRNA.

Price & Availability↑

CloneID	ORa06773
Clone ID Related Accession (Same CDS sequence)	NM_024127.2
Accession Version	NM_024127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	growth arrest and DNA damage-inducible protein GADD45 alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081795.1. On Mar 27, 2005 this sequence version replaced NM_024127.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081795.1, FQ216523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACTTTGG AGGAATTCTC GGCCGCAGAG CAGAAGATCG AAAGGATGGA CACGGTGGGC 
GATGCCCTGG AGGAAGTGCT CAGCAAGGCT CGGAGTCAGC GCACCATAAC TGTCGGCGTG 
TACGAGGCAG CCAAGCTGCT CAACGTAGAC CCGGACAACG TGGTCCTGTG CCTGCTGGCT 
GCGGATGAAG ATGACGACCG GGACGTGGCT CTGCAGATCC ATTTCACCCT CATTCGTGCT 
TTCTGTTGCG AGAACGACAT CAACATCCTG CGGGTCAGCA ACCCGGGTCG GCTGGCAGAG 
CTGTTGCTAC TGGAGAACGA CAAGAGCCCC GCTGAGAGCG GGGGCGCTGC GCAGACCCCG 
GACTTACACT GTGTGCTGGT GACGAACCCA CATTCATCAC AATGGAAGGA TCCTGCCTTA 
AGTCAACTTA TTTGTTTTTG CCGGGAAAGT CGCTACATGG ATCAGTGGGT GCCAGTGATT 
AATCTCCCCG AACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077041.2
CDS	151..648
Translation	MTLEEFSAAEQKIERMDTVGDALEEVLSKARSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVALQIHFTLIRAFCCENDINILRVSNPGRLAELLLLENDKSPAESGGAAQTPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER

Target ORF information:

RefSeq Version	NM_024127.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth arrest and DNA-damage-inducible, alpha (Gadd45a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024127.2

ORF Insert Sequence:

ATGACTTTGG AGGAATTCTC GGCCGCAGAG CAGAAGATCG AAAGGATGGA CACGGTGGGC 
GATGCCCTGG AGGAAGTGCT CAGCAAGGCT CGGAGTCAGC GCACCATAAC TGTCGGCGTG 
TACGAGGCAG CCAAGCTGCT CAACGTAGAC CCGGACAACG TGGTCCTGTG CCTGCTGGCT 
GCGGATGAAG ATGACGACCG GGACGTGGCT CTGCAGATCC ATTTCACCCT CATTCGTGCT 
TTCTGTTGCG AGAACGACAT CAACATCCTG CGGGTCAGCA ACCCGGGTCG GCTGGCAGAG 
CTGTTGCTAC TGGAGAACGA CAAGAGCCCC GCTGAGAGCG GGGGCGCTGC GCAGACCCCG 
GACTTACACT GTGTGCTGGT GACGAACCCA CATTCATCAC AATGGAAGGA TCCTGCCTTA 
AGTCAACTTA TTTGTTTTTG CCGGGAAAGT CGCTACATGG ATCAGTGGGT GCCAGTGATT 
AATCTCCCCG AACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GADD45a Promotes Active DNA Demethylation of the MMP-9 Promoter via Base Excision Repair Pathway in AGEs-treated keratinocytes and in diabetic male rat skin.
Endocrinology(2017 Dec)
Zhou L,Wang W,Yang C,Zeng T,Hu M,Wang X,Li N,Sun K,Wang C,Zhou J,Ren M,Yan L

Growth Arrest and DNA-damage-inducible Protein 45?-mediated DNA Demethylation of Voltage-dependent T-type Calcium Channel 3.2 Subunit Enhances Neuropathic Allodynia after Nerve Injury in Rats.
Anesthesiology126(6)1077-1095(2017 06)
Lai CY,Hsieh MC,Ho YC,Lee AS,Wang HH,Cheng JK,Chau YP,Peng HY

Sublytic C5b-9 triggers glomerular mesangial cell apoptosis in rat Thy-1 nephritis via Gadd45 activation mediated by Egr-1 and p300-dependent ATF3 acetylation.
Journal of molecular cell biology8(6)477-491(2016 Dec)
He F,Zhou M,Yu T,Zhao D,Zhang J,Qiu W,Lu Y,Liu Y,Wang L,Wang Y

Sex differences in Gadd45b expression and methylation in the developing rodent amygdala.
Brain research1642461-466(2016 07)
Kigar SL,Chang L,Hayne MR,Karls NT,Auger AP

Limited GADD45? expression and function in IL-1? toxicity towards insulin-producing cells.
Acta biochimica Polonica60(4)595-602(2013)
Skalniak L,Gurgul-Convey E,Okreglicka K,Skalniak A,Jura J