Slc12a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a1
Entrez Gene ID	25065
Full Name	solute carrier family 12 member 1
Synonyms	BSC1,Nkcc2
General protein information	Preferred Names solute carrier family 12 member 1 Names solute carrier family 12 member 1 Solute carrier family 12, member 1 (bumetanide-sensitive sodium-[potassium]-chloride cotransporter) bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 kidney-specific Na-K-Cl symporter solute carrier family 12 (sodium/potassium/chloride transporter), member 1 solute carrier family 12 (sodium/potassium/chloride transporters), member 1 solute carrier family 12 member 1, alternative spliced isoform
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36
Summary	has bumetamide-sensitive Na(+)-(K+)-Cl- symporter activity; may play a role in sodium and chloride transport in the kidney [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591488.1	XP_017446977.1	solute carrier family 12 member 1 isoform X1
XM_008762144.2	XP_008760366.1	solute carrier family 12 member 1 isoform X2
XM_008762142.2	XP_008760364.1	solute carrier family 12 member 1 isoform X1
NM_001270617.1	NP_001257546.1	solute carrier family 12 member 1 isoform 1
NM_001270618.1	NP_001257547.1	solute carrier family 12 member 1 isoform 2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255791.1
Homo sapiens (human)	SLC12A1	NP_000329.2
Gallus gallus (chicken)	SLC12A1	XP_004943856.1
Canis lupus familiaris (dog)	SLC12A1	XP_535467.2
Pan troglodytes (chimpanzee)	SLC12A1	NP_001104309.1
	SLC12A1	XP_001112900.1
Bos taurus (cattle)	SLC12A1	XP_005211957.1
Mus musculus (house mouse)	Slc12a1	NP_899197.2
Rattus norvegicus (Norway rat)	Slc12a1	NP_001257546.1
Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255788.1
Xenopus tropicalis (tropical clawed frog)	slc12a1	XP_004912656.1

Gene Ontology
Bibliography

Related articles in PubMed

Calcineurin inhibitor cyclosporine A activates renal Na-K-Cl cotransporters via local and systemic mechanisms.
Blankenstein KI, Borschewski A, Labes R, Paliege A, Boldt C, McCormick JA, Ellison DH, Bader M, Bachmann S, Mutig K
American journal of physiology. Renal physiology312(3)F489-F501(2017 Mar)

Renal Expression and Urinary Excretion of Na-K-2Cl Cotransporter in Obstructive Nephropathy.
Brandoni A, Torres AM
BioMed research international20177171928(2017)

Vesicle-associated Membrane Protein 3 (VAMP3) Mediates Constitutive Trafficking of the Renal Co-transporter NKCC2 in Thick Ascending Limbs: ROLE IN RENAL FUNCTION AND BLOOD PRESSURE.
Caceres PS, Mendez M, Haque MZ, Ortiz PA
The Journal of biological chemistry291(42)22063-22073(2016 Oct)

Early increasing-intensity treadmill exercise reduces neuropathic pain by preventing nociceptor collateral sprouting and disruption of chloride cotransporters homeostasis after peripheral nerve injury.
L?pez-?lvarez VM, Modol L, Navarro X, Cobianchi S
Pain156(9)1812-25(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest complex effect of cyclosporine A to activate renal Nkcc2 requires concomitant stimulation by AVP.
Title: Calcineurin inhibitor cyclosporine A activates renal Na-K-Cl cotransporters via local and systemic mechanisms.

the upregulation in the expression of NKCC2 in apical membranes during the postobstructive phase of bilateral ureteral obstruction could contribute to improving the excretion of sodium and consequently also the excretion of potassium, osmolytes, and water.
Title: Renal Expression and Urinary Excretion of Na-K-2Cl Cotransporter in Obstructive Nephropathy.

VAMP3 is required for normal NKCC2 expression, renal function, and blood pressure.
Title: Vesicle-associated Membrane Protein 3 (VAMP3) Mediates Constitutive Trafficking of the Renal Co-transporter NKCC2 in Thick Ascending Limbs: ROLE IN RENAL FUNCTION AND BLOOD PRESSURE.

This study demonstrated that disregulation nkcc1 and nkcc2 in neuropathic pain.
Title: Early increasing-intensity treadmill exercise reduces neuropathic pain by preventing nociceptor collateral sprouting and disruption of chloride cotransporters homeostasis after peripheral nerve injury.

Report tubuloglomerular and connecting tubuloglomerular feedback during inhibition of various Na transporters in the nephron.
Title: Tubuloglomerular and connecting tubuloglomerular feedback during inhibition of various Na transporters in the nephron.

The following Slc12a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09396	NM_001270617.1 Latest version!	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
ORa09399	NM_001270618.1 Latest version!	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26304	XM_008762142.2 Latest version!	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26304	XM_017591488.1 Latest version!	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa26305	XM_008762144.2 Latest version!	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a1 ( NM_001270617.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257546.1 Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa09396
Clone ID Related Accession (Same CDS sequence)	NM_001270617.1
Accession Version	NM_001270617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139960.3 and BC087017.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN579707.1, JN579708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642745 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT TGGAGTCCTT ATAATTCTTC TTTCCACCAT GGTAACCTCT 
ATCACTGGGT TGTCAACTTC TGCAATAGCA ACAAACGGGT TTGTTCGTGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATTTTT 
GCTTTCGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GGTTCGCGGA GACTGTGGTA 
GACCTTCTTA AGGAGAGTGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGAATAATC 
GGTTCCATCA CGGTGGTGAT TCTTCTAGGG ATCTCAGTAG CTGGGATGGA GTGGGAAGCT 
AAGGCTCAAG TGATTCTCCT GGTCATTCTA CTCATTGCTA TCGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA CGAGAAGAAA TCCAGAGGCT TCTTCAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAGAGGGAG AAGGCTTCTT CTCGGTCTTT 
GCTATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATTTTCAT CACCACCGTC 
GCCTACATAG GTGTTGCTAT TTGCGTAGCT GCCTGTGTGG TCCGAGACGC CACTGGGAGC 
ATGAACGACA CTGTCGTTTC TGGGATGAAT TGCAATGGGT CCGCAGCCTG CGGACTGGGC 
TACGACTTCT CAAGATGTCA GCATGAGCCA TGCCAGTATG GGTTGATGAA CAATTTTCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGCCCC CTCATCACCG CAGGGATCTT TTCGGCAACA 
CTGTCTTCGG CCCTAGCCTC CCTCGTCAGT GCACCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT TCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AACGTCATTG CACCTATCAT ATCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGCTTCC ATGCCTCGTA TGCCAAATCG CCAGGATGGA GGCCGGCATA TGGGATTTAC 
AACATGTGGG TATCTCTCTT CGGAGCCATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCGG CTGTCATCAC CTATGTCATT GAGCTCTTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTACGT GAGCGCTTTA 
GACAATGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGAGGACC CATGACAAGA CCTGCTCTCC TGGACATAAC CCATGCCTTT 
ACGAAGAATA GTGGCCTCTG TATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGTGG CATGGCTAAA AAGCAGGCCT GGCTGATGAA GAACAAAATC 
AAGGCTTTTT ATGCTGCGGT GGCGGCAGAC TGCTTCAGGG ACGGTGTGCG GAGTCTCCTT 
CAGGCCTCAG GCCTGGGGAG AATGAAGCCA AACACTCTAG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC TGAGCTTGAG AATTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGTGTAGT CATAGTCAGA ATCAGCCAAG GGTTTGACAT CTCTCCAGTT 
CTCCAGGTAC AAGATGAACT GGAGAAACTG GAACAGGAGA GACTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGACTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAACTGA ACATTACCAA GCCGGCCCCT AAAAAAGACA GCAACATCAG CACCATCCAG 
TCGATGCACG TCGGGGAGTT CAATCAGAAG CTGGTAGAAG CCAGCGCTCA GTTTAAAAAG 
AAACAGGGAA AAGGCACGAT CGATGTTTGG TGGTTATTTG ATGATGGAGG GTTGACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAG AAATGGAAAG ACTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AAATCAACCG CATTGAAGAG GAAAAAATTT CAATGGCTTC TCTTCTCAGC 
AAATTTAGGA TAAAATTTGC AGACATTCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATAGGCTCCA CGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGCCAAGTTC GACTGAACGA GCTGTTACAG 
GAGCATTCCA GAGCCGCCAA CCTCATCGTA TTGAGCCTTC CAGTGGCGAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT CCCTCCTGTG 
CTACTGGTTA GAGGAAATCA CAAGAATGTC CTGACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257546.1
CDS	216..3503
Translation	MSVNIPSNSVPSGASRFQVHVINEGHGSGAAMSDSTDPPHYEETSFGDEAQNRLKISFRPGNQECYENFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_001270617.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270617.1

ORF Insert Sequence:

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT TGGAGTCCTT ATAATTCTTC TTTCCACCAT GGTAACCTCT 
ATCACTGGGT TGTCAACTTC TGCAATAGCA ACAAACGGGT TTGTTCGTGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATTTTT 
GCTTTCGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GGTTCGCGGA GACTGTGGTA 
GACCTTCTTA AGGAGAGTGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGAATAATC 
GGTTCCATCA CGGTGGTGAT TCTTCTAGGG ATCTCAGTAG CTGGGATGGA GTGGGAAGCT 
AAGGCTCAAG TGATTCTCCT GGTCATTCTA CTCATTGCTA TCGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA CGAGAAGAAA TCCAGAGGCT TCTTCAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAGAGGGAG AAGGCTTCTT CTCGGTCTTT 
GCTATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATTTTCAT CACCACCGTC 
GCCTACATAG GTGTTGCTAT TTGCGTAGCT GCCTGTGTGG TCCGAGACGC CACTGGGAGC 
ATGAACGACA CTGTCGTTTC TGGGATGAAT TGCAATGGGT CCGCAGCCTG CGGACTGGGC 
TACGACTTCT CAAGATGTCA GCATGAGCCA TGCCAGTATG GGTTGATGAA CAATTTTCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGCCCC CTCATCACCG CAGGGATCTT TTCGGCAACA 
CTGTCTTCGG CCCTAGCCTC CCTCGTCAGT GCACCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT TCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AACGTCATTG CACCTATCAT ATCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGCTTCC ATGCCTCGTA TGCCAAATCG CCAGGATGGA GGCCGGCATA TGGGATTTAC 
AACATGTGGG TATCTCTCTT CGGAGCCATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCGG CTGTCATCAC CTATGTCATT GAGCTCTTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTACGT GAGCGCTTTA 
GACAATGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGAGGACC CATGACAAGA CCTGCTCTCC TGGACATAAC CCATGCCTTT 
ACGAAGAATA GTGGCCTCTG TATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGTGG CATGGCTAAA AAGCAGGCCT GGCTGATGAA GAACAAAATC 
AAGGCTTTTT ATGCTGCGGT GGCGGCAGAC TGCTTCAGGG ACGGTGTGCG GAGTCTCCTT 
CAGGCCTCAG GCCTGGGGAG AATGAAGCCA AACACTCTAG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC TGAGCTTGAG AATTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGTGTAGT CATAGTCAGA ATCAGCCAAG GGTTTGACAT CTCTCCAGTT 
CTCCAGGTAC AAGATGAACT GGAGAAACTG GAACAGGAGA GACTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGACTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAACTGA ACATTACCAA GCCGGCCCCT AAAAAAGACA GCAACATCAG CACCATCCAG 
TCGATGCACG TCGGGGAGTT CAATCAGAAG CTGGTAGAAG CCAGCGCTCA GTTTAAAAAG 
AAACAGGGAA AAGGCACGAT CGATGTTTGG TGGTTATTTG ATGATGGAGG GTTGACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAG AAATGGAAAG ACTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AAATCAACCG CATTGAAGAG GAAAAAATTT CAATGGCTTC TCTTCTCAGC 
AAATTTAGGA TAAAATTTGC AGACATTCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATAGGCTCCA CGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGCCAAGTTC GACTGAACGA GCTGTTACAG 
GAGCATTCCA GAGCCGCCAA CCTCATCGTA TTGAGCCTTC CAGTGGCGAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT CCCTCCTGTG 
CTACTGGTTA GAGGAAATCA CAAGAATGTC CTGACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( NM_001270618.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257547.1 Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa09399
Clone ID Related Accession (Same CDS sequence)	NM_001270618.1
Accession Version	NM_001270618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139960.3, BC087017.1 and FM059245.1. Transcript Variant: This variant (2) has an alternate exon of the same size replacing another exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini and is the same length, but it has an internal region of alternate sequence compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642745 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT GGGAGTCATC ATCATTGGCC TGAGCGTGGT AGTGACAACA 
CTCACAGGTA TCTCTATGTC TGCTATTTGC ACAAACGGAG TGGTGAGAGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATTTTT 
GCTTTCGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GGTTCGCGGA GACTGTGGTA 
GACCTTCTTA AGGAGAGTGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGAATAATC 
GGTTCCATCA CGGTGGTGAT TCTTCTAGGG ATCTCAGTAG CTGGGATGGA GTGGGAAGCT 
AAGGCTCAAG TGATTCTCCT GGTCATTCTA CTCATTGCTA TCGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA CGAGAAGAAA TCCAGAGGCT TCTTCAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAGAGGGAG AAGGCTTCTT CTCGGTCTTT 
GCTATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATTTTCAT CACCACCGTC 
GCCTACATAG GTGTTGCTAT TTGCGTAGCT GCCTGTGTGG TCCGAGACGC CACTGGGAGC 
ATGAACGACA CTGTCGTTTC TGGGATGAAT TGCAATGGGT CCGCAGCCTG CGGACTGGGC 
TACGACTTCT CAAGATGTCA GCATGAGCCA TGCCAGTATG GGTTGATGAA CAATTTTCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGCCCC CTCATCACCG CAGGGATCTT TTCGGCAACA 
CTGTCTTCGG CCCTAGCCTC CCTCGTCAGT GCACCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT TCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AACGTCATTG CACCTATCAT ATCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGCTTCC ATGCCTCGTA TGCCAAATCG CCAGGATGGA GGCCGGCATA TGGGATTTAC 
AACATGTGGG TATCTCTCTT CGGAGCCATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCGG CTGTCATCAC CTATGTCATT GAGCTCTTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTACGT GAGCGCTTTA 
GACAATGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGAGGACC CATGACAAGA CCTGCTCTCC TGGACATAAC CCATGCCTTT 
ACGAAGAATA GTGGCCTCTG TATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGTGG CATGGCTAAA AAGCAGGCCT GGCTGATGAA GAACAAAATC 
AAGGCTTTTT ATGCTGCGGT GGCGGCAGAC TGCTTCAGGG ACGGTGTGCG GAGTCTCCTT 
CAGGCCTCAG GCCTGGGGAG AATGAAGCCA AACACTCTAG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC TGAGCTTGAG AATTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGTGTAGT CATAGTCAGA ATCAGCCAAG GGTTTGACAT CTCTCCAGTT 
CTCCAGGTAC AAGATGAACT GGAGAAACTG GAACAGGAGA GACTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGACTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAACTGA ACATTACCAA GCCGGCCCCT AAAAAAGACA GCAACATCAG CACCATCCAG 
TCGATGCACG TCGGGGAGTT CAATCAGAAG CTGGTAGAAG CCAGCGCTCA GTTTAAAAAG 
AAACAGGGAA AAGGCACGAT CGATGTTTGG TGGTTATTTG ATGATGGAGG GTTGACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAG AAATGGAAAG ACTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AAATCAACCG CATTGAAGAG GAAAAAATTT CAATGGCTTC TCTTCTCAGC 
AAATTTAGGA TAAAATTTGC AGACATTCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATAGGCTCCA CGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGCCAAGTTC GACTGAACGA GCTGTTACAG 
GAGCATTCCA GAGCCGCCAA CCTCATCGTA TTGAGCCTTC CAGTGGCGAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT CCCTCCTGTG 
CTACTGGTTA GAGGAAATCA CAAGAATGTC CTGACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257547.1
CDS	216..3503
Translation	MSVNIPSNSVPSGASRFQVHVINEGHGSGAAMSDSTDPPHYEETSFGDEAQNRLKISFRPGNQECYENFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	NM_001270618.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270618.1

ORF Insert Sequence:

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT GGGAGTCATC ATCATTGGCC TGAGCGTGGT AGTGACAACA 
CTCACAGGTA TCTCTATGTC TGCTATTTGC ACAAACGGAG TGGTGAGAGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATTTTT 
GCTTTCGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GGTTCGCGGA GACTGTGGTA 
GACCTTCTTA AGGAGAGTGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGAATAATC 
GGTTCCATCA CGGTGGTGAT TCTTCTAGGG ATCTCAGTAG CTGGGATGGA GTGGGAAGCT 
AAGGCTCAAG TGATTCTCCT GGTCATTCTA CTCATTGCTA TCGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA CGAGAAGAAA TCCAGAGGCT TCTTCAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAGAGGGAG AAGGCTTCTT CTCGGTCTTT 
GCTATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATTTTCAT CACCACCGTC 
GCCTACATAG GTGTTGCTAT TTGCGTAGCT GCCTGTGTGG TCCGAGACGC CACTGGGAGC 
ATGAACGACA CTGTCGTTTC TGGGATGAAT TGCAATGGGT CCGCAGCCTG CGGACTGGGC 
TACGACTTCT CAAGATGTCA GCATGAGCCA TGCCAGTATG GGTTGATGAA CAATTTTCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGCCCC CTCATCACCG CAGGGATCTT TTCGGCAACA 
CTGTCTTCGG CCCTAGCCTC CCTCGTCAGT GCACCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT TCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AACGTCATTG CACCTATCAT ATCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGCTTCC ATGCCTCGTA TGCCAAATCG CCAGGATGGA GGCCGGCATA TGGGATTTAC 
AACATGTGGG TATCTCTCTT CGGAGCCATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCGG CTGTCATCAC CTATGTCATT GAGCTCTTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTACGT GAGCGCTTTA 
GACAATGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGAGGACC CATGACAAGA CCTGCTCTCC TGGACATAAC CCATGCCTTT 
ACGAAGAATA GTGGCCTCTG TATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGTGG CATGGCTAAA AAGCAGGCCT GGCTGATGAA GAACAAAATC 
AAGGCTTTTT ATGCTGCGGT GGCGGCAGAC TGCTTCAGGG ACGGTGTGCG GAGTCTCCTT 
CAGGCCTCAG GCCTGGGGAG AATGAAGCCA AACACTCTAG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC TGAGCTTGAG AATTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGTGTAGT CATAGTCAGA ATCAGCCAAG GGTTTGACAT CTCTCCAGTT 
CTCCAGGTAC AAGATGAACT GGAGAAACTG GAACAGGAGA GACTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGACTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAACTGA ACATTACCAA GCCGGCCCCT AAAAAAGACA GCAACATCAG CACCATCCAG 
TCGATGCACG TCGGGGAGTT CAATCAGAAG CTGGTAGAAG CCAGCGCTCA GTTTAAAAAG 
AAACAGGGAA AAGGCACGAT CGATGTTTGG TGGTTATTTG ATGATGGAGG GTTGACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAG AAATGGAAAG ACTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AAATCAACCG CATTGAAGAG GAAAAAATTT CAATGGCTTC TCTTCTCAGC 
AAATTTAGGA TAAAATTTGC AGACATTCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATAGGCTCCA CGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGCCAAGTTC GACTGAACGA GCTGTTACAG 
GAGCATTCCA GAGCCGCCAA CCTCATCGTA TTGAGCCTTC CAGTGGCGAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT CCCTCCTGTG 
CTACTGGTTA GAGGAAATCA CAAGAATGTC CTGACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_017591488.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446977.1 Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X2, mRNA.

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CloneID	ORa26304
Clone ID Related Accession (Same CDS sequence)	XM_017591488.1 , XM_008762142.2
Accession Version	XM_017591488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT CGGTGTGATT ATCATCGGCT TAGCCGTGAC AGTGACAGCC 
ATCACAGGTC TATCCACCTC TGCTATCGCC ACAAATGGAT ATGTCAGAGG AGGTCTTGGA 
GTCCTTATAA TTCTTCTTTC CACCATGGTA ACCTCTATCA CTGGGTTGTC AACTTCTGCA 
ATAGCAACAA ACGGGTTTGT TCGTGGAGGT GGAGCCTACT ACCTGATTTC CCGGAGCTTA 
GGGCCTGAGT TCGGTGGGTC AATAGGCTTG ATTTTTGCTT TCGCCAATGC AGTGGCTGTC 
GCTATGTATG TGGTGGGGTT CGCGGAGACT GTGGTAGACC TTCTTAAGGA GAGTGATTCA 
ATGATGGTGG ATCCAACCAA TGACATCCGA ATAATCGGTT CCATCACGGT GGTGATTCTT 
CTAGGGATCT CAGTAGCTGG GATGGAGTGG GAAGCTAAGG CTCAAGTGAT TCTCCTGGTC 
ATTCTACTCA TTGCTATCGC AAACTTCTTC ATTGGAACTG TCATCCCATC CAACAACGAG 
AAGAAATCCA GAGGCTTCTT CAACTACCAA GCATCTATAT TTGCGGAAAA CTTTGGGCCA 
AGCTTCACAG AGGGAGAAGG CTTCTTCTCG GTCTTTGCTA TCTTTTTCCC AGCTGCTACT 
GGGATTCTTG CTGGTGCCAA CATCTCTGGA GACCTGGAGG ATCCCCAGGA CGCCATCCCC 
AGAGGGACCA TGCTGGCCAT TTTCATCACC ACCGTCGCCT ACATAGGTGT TGCTATTTGC 
GTAGCTGCCT GTGTGGTCCG AGACGCCACT GGGAGCATGA ACGACACTGT CGTTTCTGGG 
ATGAATTGCA ATGGGTCCGC AGCCTGCGGA CTGGGCTACG ACTTCTCAAG ATGTCAGCAT 
GAGCCATGCC AGTATGGGTT GATGAACAAT TTTCAGGTCA TGAGCATGGT GTCCGGGTTC 
GGCCCCCTCA TCACCGCAGG GATCTTTTCG GCAACACTGT CTTCGGCCCT AGCCTCCCTC 
GTCAGTGCAC CCAAAGTATT CCAGGCTCTG TGCAAGGACA ACATCTTCAA AGGACTTCAG 
TTCTTCGCAA AGGGCTATGG GAAAAACAAC GAGCCCCTGA GAGGGTACTT CCTCACTTTT 
GTGATAGCCA TGGCATTCAT TCTCATCGCC GAGCTGAACG TCATTGCACC TATCATATCC 
AACTTCTTCC TGGCCTCATA TGCGCTTATT AATTTCTCCT GCTTCCATGC CTCGTATGCC 
AAATCGCCAG GATGGAGGCC GGCATATGGG ATTTACAACA TGTGGGTATC TCTCTTCGGA 
GCCATTTTGT GCTGTGCAGT CATGTTTGTC ATCAACTGGT GGGCGGCTGT CATCACCTAT 
GTCATTGAGC TCTTCCTCTA TATCTATGTG ACTTATAAGA AGCCAGATGT GAACTGGGGC 
TCCTCCACAC AGGCTCTGTC CTACGTGAGC GCTTTAGACA ATGCTCTGGA ATTAACCACA 
GTGGAAGACC ATGTGAAAAA CTTCAGACCC CAGTGCATTG TCTTAACAGG AGGACCCATG 
ACAAGACCTG CTCTCCTGGA CATAACCCAT GCCTTTACGA AGAATAGTGG CCTCTGTATC 
TGCTGCGAAG TCTTCGTGGG ACCTCGCAAG CTGTGTGTTA AAGAGATGAA CAGTGGCATG 
GCTAAAAAGC AGGCCTGGCT GATGAAGAAC AAAATCAAGG CTTTTTATGC TGCGGTGGCG 
GCAGACTGCT TCAGGGACGG TGTGCGGAGT CTCCTTCAGG CCTCAGGCCT GGGGAGAATG 
AAGCCAAACA CTCTAGTGAT TGGATACAAA AAGAACTGGA GGAAAGCTCC CTTGTCTGAG 
CTTGAGAATT ACGTGGGAAT CATACATGAC GCCTTTGACT TTGAGATTGG TGTAGTCATA 
GTCAGAATCA GCCAAGGGTT TGACATCTCT CCAGTTCTCC AGGTACAAGA TGAACTGGAG 
AAACTGGAAC AGGAGAGACT GGCTCTGGAG GCCGCAATCA AAGACAACGA CTGTGAGGAG 
GGCAAGGGGG GCATCCGAGG CTTGTTTAAG AAGGCCGGCA AACTGAACAT TACCAAGCCG 
GCCCCTAAAA AAGACAGCAA CATCAGCACC ATCCAGTCGA TGCACGTCGG GGAGTTCAAT 
CAGAAGCTGG TAGAAGCCAG CGCTCAGTTT AAAAAGAAAC AGGGAAAAGG CACGATCGAT 
GTTTGGTGGT TATTTGATGA TGGAGGGTTG ACACTGCTTA TCCCATATAT CTTGACTCTC 
AGAAAGAAAT GGAAAGACTG TAAGTTAAGA ATCTATGTTG GAGGAAAAAT CAACCGCATT 
GAAGAGGAAA AAATTTCAAT GGCTTCTCTT CTCAGCAAAT TTAGGATAAA ATTTGCAGAC 
ATTCATATAA TTGGAGACAT CAACATTAAG CCAAACAAAG AGAGCTGGAA AGTCTTTGAA 
GAGATGATTG AACCTTATAG GCTCCACGAA AGCCACAAAG ATTTGACCAC TGCTGAGAAA 
TTAAAAAGAG AAAGTCCATG GAAAATTACA GATGCAGAAC TGGAAGCAGT CAAGGAAAAG 
AGTTACCGCC AAGTTCGACT GAACGAGCTG TTACAGGAGC ATTCCAGAGC CGCCAACCTC 
ATCGTATTGA GCCTTCCAGT GGCGAGAAAA GGATCTATTT CGGATTTGTT GTACATGGCG 
TGGTTAGAAA TCCTGACCAA GAACCTCCCT CCTGTGCTAC TGGTTAGAGG AAATCACAAG 
AATGTCCTGA CATTTTACTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446977.1
CDS	286..3669
Translation	MSVNIPSNSVPSGASRFQVHVINEGHGSGAAMSDSTDPPHYEETSFGDEAQNRLKISFRPGNQECYENFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_017591488.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591488.1

ORF Insert Sequence:

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT CGGTGTGATT ATCATCGGCT TAGCCGTGAC AGTGACAGCC 
ATCACAGGTC TATCCACCTC TGCTATCGCC ACAAATGGAT ATGTCAGAGG AGGTCTTGGA 
GTCCTTATAA TTCTTCTTTC CACCATGGTA ACCTCTATCA CTGGGTTGTC AACTTCTGCA 
ATAGCAACAA ACGGGTTTGT TCGTGGAGGT GGAGCCTACT ACCTGATTTC CCGGAGCTTA 
GGGCCTGAGT TCGGTGGGTC AATAGGCTTG ATTTTTGCTT TCGCCAATGC AGTGGCTGTC 
GCTATGTATG TGGTGGGGTT CGCGGAGACT GTGGTAGACC TTCTTAAGGA GAGTGATTCA 
ATGATGGTGG ATCCAACCAA TGACATCCGA ATAATCGGTT CCATCACGGT GGTGATTCTT 
CTAGGGATCT CAGTAGCTGG GATGGAGTGG GAAGCTAAGG CTCAAGTGAT TCTCCTGGTC 
ATTCTACTCA TTGCTATCGC AAACTTCTTC ATTGGAACTG TCATCCCATC CAACAACGAG 
AAGAAATCCA GAGGCTTCTT CAACTACCAA GCATCTATAT TTGCGGAAAA CTTTGGGCCA 
AGCTTCACAG AGGGAGAAGG CTTCTTCTCG GTCTTTGCTA TCTTTTTCCC AGCTGCTACT 
GGGATTCTTG CTGGTGCCAA CATCTCTGGA GACCTGGAGG ATCCCCAGGA CGCCATCCCC 
AGAGGGACCA TGCTGGCCAT TTTCATCACC ACCGTCGCCT ACATAGGTGT TGCTATTTGC 
GTAGCTGCCT GTGTGGTCCG AGACGCCACT GGGAGCATGA ACGACACTGT CGTTTCTGGG 
ATGAATTGCA ATGGGTCCGC AGCCTGCGGA CTGGGCTACG ACTTCTCAAG ATGTCAGCAT 
GAGCCATGCC AGTATGGGTT GATGAACAAT TTTCAGGTCA TGAGCATGGT GTCCGGGTTC 
GGCCCCCTCA TCACCGCAGG GATCTTTTCG GCAACACTGT CTTCGGCCCT AGCCTCCCTC 
GTCAGTGCAC CCAAAGTATT CCAGGCTCTG TGCAAGGACA ACATCTTCAA AGGACTTCAG 
TTCTTCGCAA AGGGCTATGG GAAAAACAAC GAGCCCCTGA GAGGGTACTT CCTCACTTTT 
GTGATAGCCA TGGCATTCAT TCTCATCGCC GAGCTGAACG TCATTGCACC TATCATATCC 
AACTTCTTCC TGGCCTCATA TGCGCTTATT AATTTCTCCT GCTTCCATGC CTCGTATGCC 
AAATCGCCAG GATGGAGGCC GGCATATGGG ATTTACAACA TGTGGGTATC TCTCTTCGGA 
GCCATTTTGT GCTGTGCAGT CATGTTTGTC ATCAACTGGT GGGCGGCTGT CATCACCTAT 
GTCATTGAGC TCTTCCTCTA TATCTATGTG ACTTATAAGA AGCCAGATGT GAACTGGGGC 
TCCTCCACAC AGGCTCTGTC CTACGTGAGC GCTTTAGACA ATGCTCTGGA ATTAACCACA 
GTGGAAGACC ATGTGAAAAA CTTCAGACCC CAGTGCATTG TCTTAACAGG AGGACCCATG 
ACAAGACCTG CTCTCCTGGA CATAACCCAT GCCTTTACGA AGAATAGTGG CCTCTGTATC 
TGCTGCGAAG TCTTCGTGGG ACCTCGCAAG CTGTGTGTTA AAGAGATGAA CAGTGGCATG 
GCTAAAAAGC AGGCCTGGCT GATGAAGAAC AAAATCAAGG CTTTTTATGC TGCGGTGGCG 
GCAGACTGCT TCAGGGACGG TGTGCGGAGT CTCCTTCAGG CCTCAGGCCT GGGGAGAATG 
AAGCCAAACA CTCTAGTGAT TGGATACAAA AAGAACTGGA GGAAAGCTCC CTTGTCTGAG 
CTTGAGAATT ACGTGGGAAT CATACATGAC GCCTTTGACT TTGAGATTGG TGTAGTCATA 
GTCAGAATCA GCCAAGGGTT TGACATCTCT CCAGTTCTCC AGGTACAAGA TGAACTGGAG 
AAACTGGAAC AGGAGAGACT GGCTCTGGAG GCCGCAATCA AAGACAACGA CTGTGAGGAG 
GGCAAGGGGG GCATCCGAGG CTTGTTTAAG AAGGCCGGCA AACTGAACAT TACCAAGCCG 
GCCCCTAAAA AAGACAGCAA CATCAGCACC ATCCAGTCGA TGCACGTCGG GGAGTTCAAT 
CAGAAGCTGG TAGAAGCCAG CGCTCAGTTT AAAAAGAAAC AGGGAAAAGG CACGATCGAT 
GTTTGGTGGT TATTTGATGA TGGAGGGTTG ACACTGCTTA TCCCATATAT CTTGACTCTC 
AGAAAGAAAT GGAAAGACTG TAAGTTAAGA ATCTATGTTG GAGGAAAAAT CAACCGCATT 
GAAGAGGAAA AAATTTCAAT GGCTTCTCTT CTCAGCAAAT TTAGGATAAA ATTTGCAGAC 
ATTCATATAA TTGGAGACAT CAACATTAAG CCAAACAAAG AGAGCTGGAA AGTCTTTGAA 
GAGATGATTG AACCTTATAG GCTCCACGAA AGCCACAAAG ATTTGACCAC TGCTGAGAAA 
TTAAAAAGAG AAAGTCCATG GAAAATTACA GATGCAGAAC TGGAAGCAGT CAAGGAAAAG 
AGTTACCGCC AAGTTCGACT GAACGAGCTG TTACAGGAGC ATTCCAGAGC CGCCAACCTC 
ATCGTATTGA GCCTTCCAGT GGCGAGAAAA GGATCTATTT CGGATTTGTT GTACATGGCG 
TGGTTAGAAA TCCTGACCAA GAACCTCCCT CCTGTGCTAC TGGTTAGAGG AAATCACAAG 
AATGTCCTGA CATTTTACTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_008762142.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760364.1 Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X1, mRNA.

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CloneID	ORa26304
Clone ID Related Accession (Same CDS sequence)	XM_017591488.1 , XM_008762142.2
Accession Version	XM_008762142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT CGGTGTGATT ATCATCGGCT TAGCCGTGAC AGTGACAGCC 
ATCACAGGTC TATCCACCTC TGCTATCGCC ACAAATGGAT ATGTCAGAGG AGGTCTTGGA 
GTCCTTATAA TTCTTCTTTC CACCATGGTA ACCTCTATCA CTGGGTTGTC AACTTCTGCA 
ATAGCAACAA ACGGGTTTGT TCGTGGAGGT GGAGCCTACT ACCTGATTTC CCGGAGCTTA 
GGGCCTGAGT TCGGTGGGTC AATAGGCTTG ATTTTTGCTT TCGCCAATGC AGTGGCTGTC 
GCTATGTATG TGGTGGGGTT CGCGGAGACT GTGGTAGACC TTCTTAAGGA GAGTGATTCA 
ATGATGGTGG ATCCAACCAA TGACATCCGA ATAATCGGTT CCATCACGGT GGTGATTCTT 
CTAGGGATCT CAGTAGCTGG GATGGAGTGG GAAGCTAAGG CTCAAGTGAT TCTCCTGGTC 
ATTCTACTCA TTGCTATCGC AAACTTCTTC ATTGGAACTG TCATCCCATC CAACAACGAG 
AAGAAATCCA GAGGCTTCTT CAACTACCAA GCATCTATAT TTGCGGAAAA CTTTGGGCCA 
AGCTTCACAG AGGGAGAAGG CTTCTTCTCG GTCTTTGCTA TCTTTTTCCC AGCTGCTACT 
GGGATTCTTG CTGGTGCCAA CATCTCTGGA GACCTGGAGG ATCCCCAGGA CGCCATCCCC 
AGAGGGACCA TGCTGGCCAT TTTCATCACC ACCGTCGCCT ACATAGGTGT TGCTATTTGC 
GTAGCTGCCT GTGTGGTCCG AGACGCCACT GGGAGCATGA ACGACACTGT CGTTTCTGGG 
ATGAATTGCA ATGGGTCCGC AGCCTGCGGA CTGGGCTACG ACTTCTCAAG ATGTCAGCAT 
GAGCCATGCC AGTATGGGTT GATGAACAAT TTTCAGGTCA TGAGCATGGT GTCCGGGTTC 
GGCCCCCTCA TCACCGCAGG GATCTTTTCG GCAACACTGT CTTCGGCCCT AGCCTCCCTC 
GTCAGTGCAC CCAAAGTATT CCAGGCTCTG TGCAAGGACA ACATCTTCAA AGGACTTCAG 
TTCTTCGCAA AGGGCTATGG GAAAAACAAC GAGCCCCTGA GAGGGTACTT CCTCACTTTT 
GTGATAGCCA TGGCATTCAT TCTCATCGCC GAGCTGAACG TCATTGCACC TATCATATCC 
AACTTCTTCC TGGCCTCATA TGCGCTTATT AATTTCTCCT GCTTCCATGC CTCGTATGCC 
AAATCGCCAG GATGGAGGCC GGCATATGGG ATTTACAACA TGTGGGTATC TCTCTTCGGA 
GCCATTTTGT GCTGTGCAGT CATGTTTGTC ATCAACTGGT GGGCGGCTGT CATCACCTAT 
GTCATTGAGC TCTTCCTCTA TATCTATGTG ACTTATAAGA AGCCAGATGT GAACTGGGGC 
TCCTCCACAC AGGCTCTGTC CTACGTGAGC GCTTTAGACA ATGCTCTGGA ATTAACCACA 
GTGGAAGACC ATGTGAAAAA CTTCAGACCC CAGTGCATTG TCTTAACAGG AGGACCCATG 
ACAAGACCTG CTCTCCTGGA CATAACCCAT GCCTTTACGA AGAATAGTGG CCTCTGTATC 
TGCTGCGAAG TCTTCGTGGG ACCTCGCAAG CTGTGTGTTA AAGAGATGAA CAGTGGCATG 
GCTAAAAAGC AGGCCTGGCT GATGAAGAAC AAAATCAAGG CTTTTTATGC TGCGGTGGCG 
GCAGACTGCT TCAGGGACGG TGTGCGGAGT CTCCTTCAGG CCTCAGGCCT GGGGAGAATG 
AAGCCAAACA CTCTAGTGAT TGGATACAAA AAGAACTGGA GGAAAGCTCC CTTGTCTGAG 
CTTGAGAATT ACGTGGGAAT CATACATGAC GCCTTTGACT TTGAGATTGG TGTAGTCATA 
GTCAGAATCA GCCAAGGGTT TGACATCTCT CCAGTTCTCC AGGTACAAGA TGAACTGGAG 
AAACTGGAAC AGGAGAGACT GGCTCTGGAG GCCGCAATCA AAGACAACGA CTGTGAGGAG 
GGCAAGGGGG GCATCCGAGG CTTGTTTAAG AAGGCCGGCA AACTGAACAT TACCAAGCCG 
GCCCCTAAAA AAGACAGCAA CATCAGCACC ATCCAGTCGA TGCACGTCGG GGAGTTCAAT 
CAGAAGCTGG TAGAAGCCAG CGCTCAGTTT AAAAAGAAAC AGGGAAAAGG CACGATCGAT 
GTTTGGTGGT TATTTGATGA TGGAGGGTTG ACACTGCTTA TCCCATATAT CTTGACTCTC 
AGAAAGAAAT GGAAAGACTG TAAGTTAAGA ATCTATGTTG GAGGAAAAAT CAACCGCATT 
GAAGAGGAAA AAATTTCAAT GGCTTCTCTT CTCAGCAAAT TTAGGATAAA ATTTGCAGAC 
ATTCATATAA TTGGAGACAT CAACATTAAG CCAAACAAAG AGAGCTGGAA AGTCTTTGAA 
GAGATGATTG AACCTTATAG GCTCCACGAA AGCCACAAAG ATTTGACCAC TGCTGAGAAA 
TTAAAAAGAG AAAGTCCATG GAAAATTACA GATGCAGAAC TGGAAGCAGT CAAGGAAAAG 
AGTTACCGCC AAGTTCGACT GAACGAGCTG TTACAGGAGC ATTCCAGAGC CGCCAACCTC 
ATCGTATTGA GCCTTCCAGT GGCGAGAAAA GGATCTATTT CGGATTTGTT GTACATGGCG 
TGGTTAGAAA TCCTGACCAA GAACCTCCCT CCTGTGCTAC TGGTTAGAGG AAATCACAAG 
AATGTCCTGA CATTTTACTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760364.1
CDS	287..3670
Translation	MSVNIPSNSVPSGASRFQVHVINEGHGSGAAMSDSTDPPHYEETSFGDEAQNRLKISFRPGNQECYENFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_008762142.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762142.2

ORF Insert Sequence:

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT CGGTGTGATT ATCATCGGCT TAGCCGTGAC AGTGACAGCC 
ATCACAGGTC TATCCACCTC TGCTATCGCC ACAAATGGAT ATGTCAGAGG AGGTCTTGGA 
GTCCTTATAA TTCTTCTTTC CACCATGGTA ACCTCTATCA CTGGGTTGTC AACTTCTGCA 
ATAGCAACAA ACGGGTTTGT TCGTGGAGGT GGAGCCTACT ACCTGATTTC CCGGAGCTTA 
GGGCCTGAGT TCGGTGGGTC AATAGGCTTG ATTTTTGCTT TCGCCAATGC AGTGGCTGTC 
GCTATGTATG TGGTGGGGTT CGCGGAGACT GTGGTAGACC TTCTTAAGGA GAGTGATTCA 
ATGATGGTGG ATCCAACCAA TGACATCCGA ATAATCGGTT CCATCACGGT GGTGATTCTT 
CTAGGGATCT CAGTAGCTGG GATGGAGTGG GAAGCTAAGG CTCAAGTGAT TCTCCTGGTC 
ATTCTACTCA TTGCTATCGC AAACTTCTTC ATTGGAACTG TCATCCCATC CAACAACGAG 
AAGAAATCCA GAGGCTTCTT CAACTACCAA GCATCTATAT TTGCGGAAAA CTTTGGGCCA 
AGCTTCACAG AGGGAGAAGG CTTCTTCTCG GTCTTTGCTA TCTTTTTCCC AGCTGCTACT 
GGGATTCTTG CTGGTGCCAA CATCTCTGGA GACCTGGAGG ATCCCCAGGA CGCCATCCCC 
AGAGGGACCA TGCTGGCCAT TTTCATCACC ACCGTCGCCT ACATAGGTGT TGCTATTTGC 
GTAGCTGCCT GTGTGGTCCG AGACGCCACT GGGAGCATGA ACGACACTGT CGTTTCTGGG 
ATGAATTGCA ATGGGTCCGC AGCCTGCGGA CTGGGCTACG ACTTCTCAAG ATGTCAGCAT 
GAGCCATGCC AGTATGGGTT GATGAACAAT TTTCAGGTCA TGAGCATGGT GTCCGGGTTC 
GGCCCCCTCA TCACCGCAGG GATCTTTTCG GCAACACTGT CTTCGGCCCT AGCCTCCCTC 
GTCAGTGCAC CCAAAGTATT CCAGGCTCTG TGCAAGGACA ACATCTTCAA AGGACTTCAG 
TTCTTCGCAA AGGGCTATGG GAAAAACAAC GAGCCCCTGA GAGGGTACTT CCTCACTTTT 
GTGATAGCCA TGGCATTCAT TCTCATCGCC GAGCTGAACG TCATTGCACC TATCATATCC 
AACTTCTTCC TGGCCTCATA TGCGCTTATT AATTTCTCCT GCTTCCATGC CTCGTATGCC 
AAATCGCCAG GATGGAGGCC GGCATATGGG ATTTACAACA TGTGGGTATC TCTCTTCGGA 
GCCATTTTGT GCTGTGCAGT CATGTTTGTC ATCAACTGGT GGGCGGCTGT CATCACCTAT 
GTCATTGAGC TCTTCCTCTA TATCTATGTG ACTTATAAGA AGCCAGATGT GAACTGGGGC 
TCCTCCACAC AGGCTCTGTC CTACGTGAGC GCTTTAGACA ATGCTCTGGA ATTAACCACA 
GTGGAAGACC ATGTGAAAAA CTTCAGACCC CAGTGCATTG TCTTAACAGG AGGACCCATG 
ACAAGACCTG CTCTCCTGGA CATAACCCAT GCCTTTACGA AGAATAGTGG CCTCTGTATC 
TGCTGCGAAG TCTTCGTGGG ACCTCGCAAG CTGTGTGTTA AAGAGATGAA CAGTGGCATG 
GCTAAAAAGC AGGCCTGGCT GATGAAGAAC AAAATCAAGG CTTTTTATGC TGCGGTGGCG 
GCAGACTGCT TCAGGGACGG TGTGCGGAGT CTCCTTCAGG CCTCAGGCCT GGGGAGAATG 
AAGCCAAACA CTCTAGTGAT TGGATACAAA AAGAACTGGA GGAAAGCTCC CTTGTCTGAG 
CTTGAGAATT ACGTGGGAAT CATACATGAC GCCTTTGACT TTGAGATTGG TGTAGTCATA 
GTCAGAATCA GCCAAGGGTT TGACATCTCT CCAGTTCTCC AGGTACAAGA TGAACTGGAG 
AAACTGGAAC AGGAGAGACT GGCTCTGGAG GCCGCAATCA AAGACAACGA CTGTGAGGAG 
GGCAAGGGGG GCATCCGAGG CTTGTTTAAG AAGGCCGGCA AACTGAACAT TACCAAGCCG 
GCCCCTAAAA AAGACAGCAA CATCAGCACC ATCCAGTCGA TGCACGTCGG GGAGTTCAAT 
CAGAAGCTGG TAGAAGCCAG CGCTCAGTTT AAAAAGAAAC AGGGAAAAGG CACGATCGAT 
GTTTGGTGGT TATTTGATGA TGGAGGGTTG ACACTGCTTA TCCCATATAT CTTGACTCTC 
AGAAAGAAAT GGAAAGACTG TAAGTTAAGA ATCTATGTTG GAGGAAAAAT CAACCGCATT 
GAAGAGGAAA AAATTTCAAT GGCTTCTCTT CTCAGCAAAT TTAGGATAAA ATTTGCAGAC 
ATTCATATAA TTGGAGACAT CAACATTAAG CCAAACAAAG AGAGCTGGAA AGTCTTTGAA 
GAGATGATTG AACCTTATAG GCTCCACGAA AGCCACAAAG ATTTGACCAC TGCTGAGAAA 
TTAAAAAGAG AAAGTCCATG GAAAATTACA GATGCAGAAC TGGAAGCAGT CAAGGAAAAG 
AGTTACCGCC AAGTTCGACT GAACGAGCTG TTACAGGAGC ATTCCAGAGC CGCCAACCTC 
ATCGTATTGA GCCTTCCAGT GGCGAGAAAA GGATCTATTT CGGATTTGTT GTACATGGCG 
TGGTTAGAAA TCCTGACCAA GAACCTCCCT CCTGTGCTAC TGGTTAGAGG AAATCACAAG 
AATGTCCTGA CATTTTACTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a1 ( XM_008762144.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760366.1 Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa26305
Clone ID Related Accession (Same CDS sequence)	XM_008762144.2
Accession Version	XM_008762144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT CGGTGTGATT ATCATCGGCT TAGCCGTGAC AGTGACAGCC 
ATCACAGGTC TATCCACCTC TGCTATCGCC ACAAATGGAT ATGTCAGAGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATTTTT 
GCTTTCGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GGTTCGCGGA GACTGTGGTA 
GACCTTCTTA AGGAGAGTGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGAATAATC 
GGTTCCATCA CGGTGGTGAT TCTTCTAGGG ATCTCAGTAG CTGGGATGGA GTGGGAAGCT 
AAGGCTCAAG TGATTCTCCT GGTCATTCTA CTCATTGCTA TCGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA CGAGAAGAAA TCCAGAGGCT TCTTCAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAGAGGGAG AAGGCTTCTT CTCGGTCTTT 
GCTATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATTTTCAT CACCACCGTC 
GCCTACATAG GTGTTGCTAT TTGCGTAGCT GCCTGTGTGG TCCGAGACGC CACTGGGAGC 
ATGAACGACA CTGTCGTTTC TGGGATGAAT TGCAATGGGT CCGCAGCCTG CGGACTGGGC 
TACGACTTCT CAAGATGTCA GCATGAGCCA TGCCAGTATG GGTTGATGAA CAATTTTCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGCCCC CTCATCACCG CAGGGATCTT TTCGGCAACA 
CTGTCTTCGG CCCTAGCCTC CCTCGTCAGT GCACCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT TCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AACGTCATTG CACCTATCAT ATCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGCTTCC ATGCCTCGTA TGCCAAATCG CCAGGATGGA GGCCGGCATA TGGGATTTAC 
AACATGTGGG TATCTCTCTT CGGAGCCATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCGG CTGTCATCAC CTATGTCATT GAGCTCTTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTACGT GAGCGCTTTA 
GACAATGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGAGGACC CATGACAAGA CCTGCTCTCC TGGACATAAC CCATGCCTTT 
ACGAAGAATA GTGGCCTCTG TATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGTGG CATGGCTAAA AAGCAGGCCT GGCTGATGAA GAACAAAATC 
AAGGCTTTTT ATGCTGCGGT GGCGGCAGAC TGCTTCAGGG ACGGTGTGCG GAGTCTCCTT 
CAGGCCTCAG GCCTGGGGAG AATGAAGCCA AACACTCTAG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC TGAGCTTGAG AATTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGTGTAGT CATAGTCAGA ATCAGCCAAG GGTTTGACAT CTCTCCAGTT 
CTCCAGGTAC AAGATGAACT GGAGAAACTG GAACAGGAGA GACTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGACTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAACTGA ACATTACCAA GCCGGCCCCT AAAAAAGACA GCAACATCAG CACCATCCAG 
TCGATGCACG TCGGGGAGTT CAATCAGAAG CTGGTAGAAG CCAGCGCTCA GTTTAAAAAG 
AAACAGGGAA AAGGCACGAT CGATGTTTGG TGGTTATTTG ATGATGGAGG GTTGACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAG AAATGGAAAG ACTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AAATCAACCG CATTGAAGAG GAAAAAATTT CAATGGCTTC TCTTCTCAGC 
AAATTTAGGA TAAAATTTGC AGACATTCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATAGGCTCCA CGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGCCAAGTTC GACTGAACGA GCTGTTACAG 
GAGCATTCCA GAGCCGCCAA CCTCATCGTA TTGAGCCTTC CAGTGGCGAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT CCCTCCTGTG 
CTACTGGTTA GAGGAAATCA CAAGAATGTC CTGACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760366.1
CDS	287..3574
Translation	MSVNIPSNSVPSGASRFQVHVINEGHGSGAAMSDSTDPPHYEETSFGDEAQNRLKISFRPGNQECYENFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLAVTVTAITGLSTSAIATNGYVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_008762144.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 1 (Slc12a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762144.2

ORF Insert Sequence:

ATGTCAGTCA ACATCCCTTC CAACTCAGTG CCCAGTGGTG CCAGTCGTTT CCAAGTCCAT 
GTTATAAATG AGGGCCATGG CAGTGGTGCA GCCATGAGTG ACAGCACGGA CCCCCCACAT 
TATGAAGAGA CCTCTTTCGG GGATGAAGCC CAAAACAGAC TGAAAATCAG CTTTAGGCCT 
GGGAATCAGG AGTGCTATGA AAATTTTCTC CAAACTGGAG AGACTGCTAA AACAGACACC 
ACGTTTCACG CCTATGACTC TCACACAAAC ACATATTACC TACAAACCTT TGGCCACAAC 
ACCATGGACG CCGTCCCCAA GATTGAGTAC TACCGCAACA CCGGCAGTGT GAGCGGGCCC 
AAGGTCAACC GGCCGAGCCT GCAGGAGATC CACGAGCAGC TAGCTAAGAA TGTGGCTGTG 
GCCCCTGGTT CAGCTGACAG GGTTGCTAAT GGAGATGGGA TGCCTGGAGA TGAGCAAGCA 
GAAAACAAGG AAGAAGACGT GACTGGTGTT GTGAAGTTTG GATGGGTGAA AGGTGTGCTG 
GTGAGGTGCA TGCTGAACAT CTGGGGAGTC ATGCTCTTCA TTCGCCTCTC CTGGATTGTA 
GGAGAAGCGG GAATTGGTCT CGGTGTGATT ATCATCGGCT TAGCCGTGAC AGTGACAGCC 
ATCACAGGTC TATCCACCTC TGCTATCGCC ACAAATGGAT ATGTCAGAGG AGGTGGAGCC 
TACTACCTGA TTTCCCGGAG CTTAGGGCCT GAGTTCGGTG GGTCAATAGG CTTGATTTTT 
GCTTTCGCCA ATGCAGTGGC TGTCGCTATG TATGTGGTGG GGTTCGCGGA GACTGTGGTA 
GACCTTCTTA AGGAGAGTGA TTCAATGATG GTGGATCCAA CCAATGACAT CCGAATAATC 
GGTTCCATCA CGGTGGTGAT TCTTCTAGGG ATCTCAGTAG CTGGGATGGA GTGGGAAGCT 
AAGGCTCAAG TGATTCTCCT GGTCATTCTA CTCATTGCTA TCGCAAACTT CTTCATTGGA 
ACTGTCATCC CATCCAACAA CGAGAAGAAA TCCAGAGGCT TCTTCAACTA CCAAGCATCT 
ATATTTGCGG AAAACTTTGG GCCAAGCTTC ACAGAGGGAG AAGGCTTCTT CTCGGTCTTT 
GCTATCTTTT TCCCAGCTGC TACTGGGATT CTTGCTGGTG CCAACATCTC TGGAGACCTG 
GAGGATCCCC AGGACGCCAT CCCCAGAGGG ACCATGCTGG CCATTTTCAT CACCACCGTC 
GCCTACATAG GTGTTGCTAT TTGCGTAGCT GCCTGTGTGG TCCGAGACGC CACTGGGAGC 
ATGAACGACA CTGTCGTTTC TGGGATGAAT TGCAATGGGT CCGCAGCCTG CGGACTGGGC 
TACGACTTCT CAAGATGTCA GCATGAGCCA TGCCAGTATG GGTTGATGAA CAATTTTCAG 
GTCATGAGCA TGGTGTCCGG GTTCGGCCCC CTCATCACCG CAGGGATCTT TTCGGCAACA 
CTGTCTTCGG CCCTAGCCTC CCTCGTCAGT GCACCCAAAG TATTCCAGGC TCTGTGCAAG 
GACAACATCT TCAAAGGACT TCAGTTCTTC GCAAAGGGCT ATGGGAAAAA CAACGAGCCC 
CTGAGAGGGT ACTTCCTCAC TTTTGTGATA GCCATGGCAT TCATTCTCAT CGCCGAGCTG 
AACGTCATTG CACCTATCAT ATCCAACTTC TTCCTGGCCT CATATGCGCT TATTAATTTC 
TCCTGCTTCC ATGCCTCGTA TGCCAAATCG CCAGGATGGA GGCCGGCATA TGGGATTTAC 
AACATGTGGG TATCTCTCTT CGGAGCCATT TTGTGCTGTG CAGTCATGTT TGTCATCAAC 
TGGTGGGCGG CTGTCATCAC CTATGTCATT GAGCTCTTCC TCTATATCTA TGTGACTTAT 
AAGAAGCCAG ATGTGAACTG GGGCTCCTCC ACACAGGCTC TGTCCTACGT GAGCGCTTTA 
GACAATGCTC TGGAATTAAC CACAGTGGAA GACCATGTGA AAAACTTCAG ACCCCAGTGC 
ATTGTCTTAA CAGGAGGACC CATGACAAGA CCTGCTCTCC TGGACATAAC CCATGCCTTT 
ACGAAGAATA GTGGCCTCTG TATCTGCTGC GAAGTCTTCG TGGGACCTCG CAAGCTGTGT 
GTTAAAGAGA TGAACAGTGG CATGGCTAAA AAGCAGGCCT GGCTGATGAA GAACAAAATC 
AAGGCTTTTT ATGCTGCGGT GGCGGCAGAC TGCTTCAGGG ACGGTGTGCG GAGTCTCCTT 
CAGGCCTCAG GCCTGGGGAG AATGAAGCCA AACACTCTAG TGATTGGATA CAAAAAGAAC 
TGGAGGAAAG CTCCCTTGTC TGAGCTTGAG AATTACGTGG GAATCATACA TGACGCCTTT 
GACTTTGAGA TTGGTGTAGT CATAGTCAGA ATCAGCCAAG GGTTTGACAT CTCTCCAGTT 
CTCCAGGTAC AAGATGAACT GGAGAAACTG GAACAGGAGA GACTGGCTCT GGAGGCCGCA 
ATCAAAGACA ACGACTGTGA GGAGGGCAAG GGGGGCATCC GAGGCTTGTT TAAGAAGGCC 
GGCAAACTGA ACATTACCAA GCCGGCCCCT AAAAAAGACA GCAACATCAG CACCATCCAG 
TCGATGCACG TCGGGGAGTT CAATCAGAAG CTGGTAGAAG CCAGCGCTCA GTTTAAAAAG 
AAACAGGGAA AAGGCACGAT CGATGTTTGG TGGTTATTTG ATGATGGAGG GTTGACACTG 
CTTATCCCAT ATATCTTGAC TCTCAGAAAG AAATGGAAAG ACTGTAAGTT AAGAATCTAT 
GTTGGAGGAA AAATCAACCG CATTGAAGAG GAAAAAATTT CAATGGCTTC TCTTCTCAGC 
AAATTTAGGA TAAAATTTGC AGACATTCAT ATAATTGGAG ACATCAACAT TAAGCCAAAC 
AAAGAGAGCT GGAAAGTCTT TGAAGAGATG ATTGAACCTT ATAGGCTCCA CGAAAGCCAC 
AAAGATTTGA CCACTGCTGA GAAATTAAAA AGAGAAAGTC CATGGAAAAT TACAGATGCA 
GAACTGGAAG CAGTCAAGGA AAAGAGTTAC CGCCAAGTTC GACTGAACGA GCTGTTACAG 
GAGCATTCCA GAGCCGCCAA CCTCATCGTA TTGAGCCTTC CAGTGGCGAG AAAAGGATCT 
ATTTCGGATT TGTTGTACAT GGCGTGGTTA GAAATCCTGA CCAAGAACCT CCCTCCTGTG 
CTACTGGTTA GAGGAAATCA CAAGAATGTC CTGACATTTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Calcineurin inhibitor cyclosporine A activates renal Na-K-Cl cotransporters via local and systemic mechanisms.
American journal of physiology. Renal physiology312(3)F489-F501(2017 Mar)
Blankenstein KI,Borschewski A,Labes R,Paliege A,Boldt C,McCormick JA,Ellison DH,Bader M,Bachmann S,Mutig K

Renal Expression and Urinary Excretion of Na-K-2Cl Cotransporter in Obstructive Nephropathy.
BioMed research international20177171928(2017)
Brandoni A,Torres AM

Vesicle-associated Membrane Protein 3 (VAMP3) Mediates Constitutive Trafficking of the Renal Co-transporter NKCC2 in Thick Ascending Limbs: ROLE IN RENAL FUNCTION AND BLOOD PRESSURE.
The Journal of biological chemistry291(42)22063-22073(2016 Oct)
Caceres PS,Mendez M,Haque MZ,Ortiz PA

Early increasing-intensity treadmill exercise reduces neuropathic pain by preventing nociceptor collateral sprouting and disruption of chloride cotransporters homeostasis after peripheral nerve injury.
Pain156(9)1812-25(2015 Sep)
L?pez-?lvarez VM,Modol L,Navarro X,Cobianchi S