Hk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hk2
Entrez Gene ID	25059
Full Name	hexokinase 2
General protein information	Preferred Names hexokinase 2 Names hexokinase-2 HK II hexokinase type II type II hexokinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	enzyme involved in the phosphorylation of glucose to produce glucose-6-phosphate; may be involved in the increased rate of glycolysis seen in cancer cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006236711.3	XP_006236773.1	hexokinase-2 isoform X1
NM_012735.2	NP_036867.1	hexokinase-2

Pathways from BioSystems

Homologies

	HK2	XP_001111706.1
Caenorhabditis elegans (roundworm)	F14B4.2	NP_001021107.1
Pan troglodytes (chimpanzee)	HK2	XP_001162535.1
Gallus gallus (chicken)	HK2	NP_989543.1
Danio rerio (zebrafish)	hk2	NP_998231.1
Mus musculus (house mouse)	Hk2	NP_038848.1
Oryza sativa (rice)	Os01g0190400	NP_001042264.1
Xenopus tropicalis (tropical clawed frog)	LOC100485269	XP_002935347.1
Homo sapiens (human)	HK2	NP_000180.2
Rattus norvegicus (Norway rat)	Hk2	NP_036867.1
Canis lupus familiaris (dog)	HK2	XP_005630567.1
Bos taurus (cattle)	HK2	XP_002691235.1

Gene Ontology
Bibliography

Related articles in PubMed

Hexokinase 2-dependent hyperglycolysis driving microglial activation contributes to ischemic brain injury.
Li Y, Lu B, Sheng L, Zhu Z, Sun H, Zhou Y, Yang Y, Xue D, Chen W, Tian X, Du Y, Yan M, Zhu W, Xing F, Li K, Lin S, Qiu P, Su X, Huang Y, Yan G, Yin W
Journal of neurochemistry144(2)186-200(2018 Jan)

The Catalytic Inactivation of the N-half of Human Hexokinase 2 and Structural and Biochemical Characterization of its Mitochondrial Conformation.
Nawaz MH, Ferreira JC, Nedyalkova L, Zhu H, Carrasco-L?pez C, Kirmizialtin S, Rabeh WM
Bioscience reports(2018 Jan)

Inhibition of
Zeng X, Liu N, Zhang J, Wang L, Zhang Z, Zhu J, Li Q, Wang Y
Bioscience reports37(4)(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the Akt/HK2 pathway is likely to play a role in the development of a cardioprotective phenotype of CNH by preventing the detachment of HK2 from mitochondria at reperfusion period and decreases the Bax/Bcl-2 ratio during I/R insult, thereby lowering the probability of apoptosis activation in the mitochondrial compartment.
Title: Adaptation to chronic continuous hypoxia potentiates Akt/HK2 anti-apoptotic pathway during brief myocardial ischemia/reperfusion insult.

HK-II binds TORC1 as a decoy substrate and provides a previously unrecognized mechanism for switching cells from a metabolic economy, based on plentiful energy, to one of conservation, under starvation
Title: Hexokinase-II positively regulates glucose starvation-induced autophagy through TORC1 inhibition.

The activation of the hexokinase-2/hypoxia-inducible factor-1alpha axis appears to be the key mediator for neointimal lesion formation in a model of pulmonary arterial hypertension.
Title: Increased expression of hypoxia-inducible factor-1? in proliferating neointimal lesions in a rat model of pulmonary arterial hypertension.

Inhibition of mitochondrial HK2 loss during ischemia could be an important mechanism responsible for the cardioprotection mediated by ischemic preconditioning and other pretreatments.
Title: Extent of mitochondrial hexokinase II dissociation during ischemia correlates with mitochondrial cytochrome c release, reactive oxygen species production, and infarct size on reperfusion.

HKI remains strongly bound to mitochondria, whereas HKII translocates between mitochondria and the cytosol in response to glucose, G-6-P and Akt, but not ATP.
Title: Subcellular localization of hexokinases I and II directs the metabolic fate of glucose.

The following Hk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13290	NM_012735.2 Latest version!	Rattus norvegicus hexokinase 2 (Hk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
ORa27370	XM_006236711.3 Latest version!	Rattus norvegicus hexokinase 2 (Hk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hk2 ( NM_012735.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036867.1 Rattus norvegicus hexokinase 2 (Hk2), mRNA.

Price & Availability↑

CloneID	ORa13290
Clone ID Related Accession (Same CDS sequence)	NM_012735.2
Accession Version	NM_012735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	hexokinase-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M68971.1. On Aug 30, 2012 this sequence version replaced NM_012735.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M68971.1, AF027179.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATCGCCT CGCATATGAT CGCCTGCTTA TTCACGGAGC TCAACCAAAA CCAAGTGCAG 
AAGGTTGACC AATTTCTCTA CCACATGCGT CTCTCAGATG AGACCCTTCT GGAGATTTCT 
AGGCGGTTCC GGAAGGAGAT GGAGAAAGGG CTAGGAGCTA CCACGCACCC TACAGCAGCT 
GTGAAAATGT TGCCTACCTT TGTGAGGTCA ACTCCGGATG GGACAGAACA TGGGGAGTTC 
CTGGCTCTGG ATCTTGGAGG AACCAACTTC CGTGTGCTCC GAGTAAGGGT GACGGACAAT 
GGCCTCCAGA GAGTGGAGAT GGAGAACCAG ATCTACGCCA TCCCTGAGGA CATCATGCGG 
GGCAGTGGAA CCCAGCTGTT TGACCACATC GCCGAATGCC TGGCCAACTT CATGGACAAG 
CTACAAATCA AAGAGAAGAA GCTCCCTCTG GGTTTCACCT TCTCGTTCCC CTGCCACCAG 
ACAAAACTGG ATGAGAGTTT TTTGGTCTCG TGGACTAAGG GGTTCAAGTC CAGTGGCGTG 
GAAGGCAGAG ATGTGGTGGA CCTGATCCGG AAGGCTATCC AGCGCAGAGG GGACTTTGAC 
ATTGACATTG TGGCCGTGGT GAATGACACA GTTGGGACCA TGATGACTTG TGGCTATGAT 
GATCAGAACT GCGAGATTGG TCTCATTGTG GGCACTGGCA GCAACGCCTG CTACATGGAG 
GAAATGCGTC ATATTGACAT GGTGGAGGGA GATGAGGGGC GCATGTGCAT CAACATGGAG 
TGGGGAGCCT TTGGGGACGA CGGTACACTC AATGACATCC GAACCGAGTT TGACCGAGAG 
ATCGACATGG GCTCGCTGAA CCCTGGGAAG CAGCTGTTTG AGAAGATGAT TAGCGGGATG 
TACATGGGGG AGCTGGTCAG GCTCATCCTG GTGAAGATGG CCAAGGCAGA GCTGTTGTTC 
CAAGGGAAAC TCAGCCCAGA ACTCCTTACC ACTGGCTCCT TCGAGACCAA AGATGTCTCG 
GATATTGAAG AGGATAAGGA TGGAATCGAG AAGGCCTACC AAATCCTGAT GCGCCTGGGT 
CTGAATCCAT TGCAGGAGGA TTGTGTGGCC ACGCACCGAA TCTGCCAGAT TGTGTCCACG 
CGCTCGGCCA GTCTGTGCGC AGCCACCCTG GCCGCGGTGC TGTGGCGAAT CAAAGAGAAC 
AAGGGCGAGG AGCGACTTCG CTCCACCATC GGTGTCGATG GCTCCGTCTA CAAGAAACAT 
CCCCATTTTG CCAAGCGTCT CCATAAGGCA GTGAGGAGGC TGGTGCCCGA CTGTGATGTC 
CGCTTCCTCC GCTCTGAGGA TGGCAGCGGC AAGGGGGCTG CTATGGTGAC GGCGGTGGCT 
TACCGTCTGG CTGACCAACA CCGGGCCCGC CAGAAGACCC TGGAGTCTCT GAAGCTGAGC 
CACGAGCAGC TTCTGGAGGT TAAGAGAAGA ATGAAGGTGG AAATGGAGCA GGGTCTGAGC 
AAGGAGACGC ATGCGGTCGC CCCTGTGAAG ATGCTGCCCA CTTACGTGTG TGCCACTCCA 
GATGGCACAG AGAAAGGAGA CTTCTTGGCC TTGGATCTTG GAGGAACAAA CTTCCGGGTC 
CTGCTGGTGC GTGTGCGTAA TGGCAAGCGG AGGGGCGTGG AGATGCATAA CAAGATCTAC 
TCCATCCCAC AGGAGGTTAT GCATGGCACT GGGGAAGAGC TCTTCGACCA CATTGTCCAG 
TGCATTGCGG ACTTCCTGGA GTACATGGGC ATGAAGGGCG TGTCCCTGCC TTTGGGTTTC 
ACATTCTCCT TCCCTTGCCA GCAGAACAGC CTAGACCAGA GCATCCTCCT CAAGTGGACA 
AAGGGATTCA AGGCATCTGG CTGCGAGGGT GAGGATGTGG TCACCTTGCT GAAGGAAGCG 
ATTCACCGGC GAGAGGAGTT TGACCTGGAT GTGGTTGCCG TGGTGAATGA CACAGTTGGG 
ACTATGATGA CTTGTGGCTA CGAAGACCCT CACTGTGAAG TTGGCCTCAT TGTTGGCACC 
GGAAGCAACG CCTGCTACAT GGAAGAGATG CGTAATGTGG AGCTGGTGGA CGGAGAGGAG 
GGACGGATGT GTGTCAACAT GGAGTGGGGA GCATTTGGGG ACAATGGCTG CCTGGATGAC 
TTGCGGACCG TGTTTGATGT TGCTGTGGAT GAGCTTTCTC TCAACCCTGG CAAACAGAGG 
TTCGAGAAGA TGATCAGCGG CATGTACTTG GGAGAGATTG TGCGCAACAT TCTCATCGAT 
TTCACGAAGC GGGGGCTGCT CTTCCGAGGC CGCATCTCAG AGCGCCTCAA GACAAGGGGA 
ATCTTTGAAA CTAAGTTCCT GTCTCAGATA GAGAGCGACT GCCTAGCCCT GCTACAGGTT 
CGTGCCATCC TGCGCCACCT AGGGCTGGAG AGCACGTGCG ATGACAGCAT CATCGTGAAG 
GAGGTGTGCA CTGTGGTTGC CCGGCGCGCT GCACAGCTCT GTGGCGCAGG CATGGCCGCC 
GTAGTGGACA AGATAAGAGA GAACCGTGGG CTGGACAACC TCAAAGTGAC AGTGGGCGTG 
GACGGGACTC TGTATAAGCT TCATCCTCAC TTTGCCAAGG TCATGCATGA GACGGTGAGA 
GATCTGGCTC CGAAATGTGA CGTGTCCTTC CTGGAATCCG AGGACGGCAG TGGGAAGGGA 
GCAGCTCTCA TCACTGCCGT GGCCTGCCGC ATCCGGGAGG CTGGGCAGAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036867.1
CDS	197..2950
Translation	MIASHMIACLFTELNQNQVQKVDQFLYHMRLSDETLLEISRRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLRVRVTDNGLQRVEMENQIYAIPEDIMRGSGTQLFDHIAECLANFMDKLQIKEKKLPLGFTFSFPCHQTKLDESFLVSWTKGFKSSGVEGRDVVDLIRKAIQRRGDFDIDIVAVVNDTVGTMMTCGYDDQNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGTLNDIRTEFDREIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKAELLFQGKLSPELLTTGSFETKDVSDIEEDKDGIEKAYQILMRLGLNPLQEDCVATHRICQIVSTRSASLCAATLAAVLWRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKAVRRLVPDCDVRFLRSEDGSGKGAAMVTAVAYRLADQHRARQKTLESLKLSHEQLLEVKRRMKVEMEQGLSKETHAVAPVKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKRRGVEMHNKIYSIPQEVMHGTGEELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDQSILLKWTKGFKASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGYEDPHCEVGLIVGTGSNACYMEEMRNVELVDGEEGRMCVNMEWGAFGDNGCLDDLRTVFDVAVDELSLNPGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALLQVRAILRHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAAVVDKIRENRGLDNLKVTVGVDGTLYKLHPHFAKVMHETVRDLAPKCDVSFLESEDGSGKGAALITAVACRIREAGQR

Target ORF information:

RefSeq Version	NM_012735.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hexokinase 2 (Hk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012735.2

ORF Insert Sequence:

ATGATCGCCT CGCATATGAT CGCCTGCTTA TTCACGGAGC TCAACCAAAA CCAAGTGCAG 
AAGGTTGACC AATTTCTCTA CCACATGCGT CTCTCAGATG AGACCCTTCT GGAGATTTCT 
AGGCGGTTCC GGAAGGAGAT GGAGAAAGGG CTAGGAGCTA CCACGCACCC TACAGCAGCT 
GTGAAAATGT TGCCTACCTT TGTGAGGTCA ACTCCGGATG GGACAGAACA TGGGGAGTTC 
CTGGCTCTGG ATCTTGGAGG AACCAACTTC CGTGTGCTCC GAGTAAGGGT GACGGACAAT 
GGCCTCCAGA GAGTGGAGAT GGAGAACCAG ATCTACGCCA TCCCTGAGGA CATCATGCGG 
GGCAGTGGAA CCCAGCTGTT TGACCACATC GCCGAATGCC TGGCCAACTT CATGGACAAG 
CTACAAATCA AAGAGAAGAA GCTCCCTCTG GGTTTCACCT TCTCGTTCCC CTGCCACCAG 
ACAAAACTGG ATGAGAGTTT TTTGGTCTCG TGGACTAAGG GGTTCAAGTC CAGTGGCGTG 
GAAGGCAGAG ATGTGGTGGA CCTGATCCGG AAGGCTATCC AGCGCAGAGG GGACTTTGAC 
ATTGACATTG TGGCCGTGGT GAATGACACA GTTGGGACCA TGATGACTTG TGGCTATGAT 
GATCAGAACT GCGAGATTGG TCTCATTGTG GGCACTGGCA GCAACGCCTG CTACATGGAG 
GAAATGCGTC ATATTGACAT GGTGGAGGGA GATGAGGGGC GCATGTGCAT CAACATGGAG 
TGGGGAGCCT TTGGGGACGA CGGTACACTC AATGACATCC GAACCGAGTT TGACCGAGAG 
ATCGACATGG GCTCGCTGAA CCCTGGGAAG CAGCTGTTTG AGAAGATGAT TAGCGGGATG 
TACATGGGGG AGCTGGTCAG GCTCATCCTG GTGAAGATGG CCAAGGCAGA GCTGTTGTTC 
CAAGGGAAAC TCAGCCCAGA ACTCCTTACC ACTGGCTCCT TCGAGACCAA AGATGTCTCG 
GATATTGAAG AGGATAAGGA TGGAATCGAG AAGGCCTACC AAATCCTGAT GCGCCTGGGT 
CTGAATCCAT TGCAGGAGGA TTGTGTGGCC ACGCACCGAA TCTGCCAGAT TGTGTCCACG 
CGCTCGGCCA GTCTGTGCGC AGCCACCCTG GCCGCGGTGC TGTGGCGAAT CAAAGAGAAC 
AAGGGCGAGG AGCGACTTCG CTCCACCATC GGTGTCGATG GCTCCGTCTA CAAGAAACAT 
CCCCATTTTG CCAAGCGTCT CCATAAGGCA GTGAGGAGGC TGGTGCCCGA CTGTGATGTC 
CGCTTCCTCC GCTCTGAGGA TGGCAGCGGC AAGGGGGCTG CTATGGTGAC GGCGGTGGCT 
TACCGTCTGG CTGACCAACA CCGGGCCCGC CAGAAGACCC TGGAGTCTCT GAAGCTGAGC 
CACGAGCAGC TTCTGGAGGT TAAGAGAAGA ATGAAGGTGG AAATGGAGCA GGGTCTGAGC 
AAGGAGACGC ATGCGGTCGC CCCTGTGAAG ATGCTGCCCA CTTACGTGTG TGCCACTCCA 
GATGGCACAG AGAAAGGAGA CTTCTTGGCC TTGGATCTTG GAGGAACAAA CTTCCGGGTC 
CTGCTGGTGC GTGTGCGTAA TGGCAAGCGG AGGGGCGTGG AGATGCATAA CAAGATCTAC 
TCCATCCCAC AGGAGGTTAT GCATGGCACT GGGGAAGAGC TCTTCGACCA CATTGTCCAG 
TGCATTGCGG ACTTCCTGGA GTACATGGGC ATGAAGGGCG TGTCCCTGCC TTTGGGTTTC 
ACATTCTCCT TCCCTTGCCA GCAGAACAGC CTAGACCAGA GCATCCTCCT CAAGTGGACA 
AAGGGATTCA AGGCATCTGG CTGCGAGGGT GAGGATGTGG TCACCTTGCT GAAGGAAGCG 
ATTCACCGGC GAGAGGAGTT TGACCTGGAT GTGGTTGCCG TGGTGAATGA CACAGTTGGG 
ACTATGATGA CTTGTGGCTA CGAAGACCCT CACTGTGAAG TTGGCCTCAT TGTTGGCACC 
GGAAGCAACG CCTGCTACAT GGAAGAGATG CGTAATGTGG AGCTGGTGGA CGGAGAGGAG 
GGACGGATGT GTGTCAACAT GGAGTGGGGA GCATTTGGGG ACAATGGCTG CCTGGATGAC 
TTGCGGACCG TGTTTGATGT TGCTGTGGAT GAGCTTTCTC TCAACCCTGG CAAACAGAGG 
TTCGAGAAGA TGATCAGCGG CATGTACTTG GGAGAGATTG TGCGCAACAT TCTCATCGAT 
TTCACGAAGC GGGGGCTGCT CTTCCGAGGC CGCATCTCAG AGCGCCTCAA GACAAGGGGA 
ATCTTTGAAA CTAAGTTCCT GTCTCAGATA GAGAGCGACT GCCTAGCCCT GCTACAGGTT 
CGTGCCATCC TGCGCCACCT AGGGCTGGAG AGCACGTGCG ATGACAGCAT CATCGTGAAG 
GAGGTGTGCA CTGTGGTTGC CCGGCGCGCT GCACAGCTCT GTGGCGCAGG CATGGCCGCC 
GTAGTGGACA AGATAAGAGA GAACCGTGGG CTGGACAACC TCAAAGTGAC AGTGGGCGTG 
GACGGGACTC TGTATAAGCT TCATCCTCAC TTTGCCAAGG TCATGCATGA GACGGTGAGA 
GATCTGGCTC CGAAATGTGA CGTGTCCTTC CTGGAATCCG AGGACGGCAG TGGGAAGGGA 
GCAGCTCTCA TCACTGCCGT GGCCTGCCGC ATCCGGGAGG CTGGGCAGAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hk2 ( XM_006236711.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236773.1 Rattus norvegicus hexokinase 2 (Hk2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27370
Clone ID Related Accession (Same CDS sequence)	XM_006236711.3
Accession Version	XM_006236711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hexokinase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC AGATCTACGC CATCCCTGAG GACATCATGC GGGGCAGTGG AACCCAGCTG 
TTTGACCACA TCGCCGAATG CCTGGCCAAC TTCATGGACA AGCTACAAAT CAAAGAGAAG 
AAGCTCCCTC TGGGTTTCAC CTTCTCGTTC CCCTGCCACC AGACAAAACT GGATGAGAGT 
TTTTTGGTCT CGTGGACTAA GGGGTTCAAG TCCAGTGGCG TGGAAGGCAG AGATGTGGTG 
GACCTGATCC GGAAGGCTAT CCAGCGCAGA GGGGACTTTG ACATTGACAT TGTGGCCGTG 
GTGAATGACA CAGTTGGGAC CATGATGACT TGTGGCTATG ATGATCAGAA CTGCGAGATT 
GGTCTCATTG TGGGCACTGG CAGCAACGCC TGCTACATGG AGGAAATGCG TCATATTGAC 
ATGGTGGAGG GAGATGAGGG GCGCATGTGC ATCAACATGG AGTGGGGAGC CTTTGGGGAC 
GACGGTACAC TCAATGACAT CCGAACCGAG TTTGACCGAG AGATCGACAT GGGCTCGCTG 
AACCCTGGGA AGCAGCTGTT TGAGAAGATG ATTAGCGGGA TGTACATGGG GGAGCTGGTC 
AGGCTCATCC TGGTGAAGAT GGCCAAGGCA GAGCTGTTGT TCCAAGGGAA ACTCAGCCCA 
GAACTCCTTA CCACTGGCTC CTTCGAGACC AAAGATGTCT CGGATATTGA AGAGGATAAG 
GATGGAATCG AGAAGGCCTA CCAAATCCTG ATGCGCCTGG GTCTGAATCC ATTGCAGGAG 
GATTGTGTGG CCACGCACCG AATCTGCCAG ATTGTGTCCA CGCGCTCGGC CAGTCTGTGC 
GCAGCCACCC TGGCCGCGGT GCTGTGGCGA ATCAAAGAGA ACAAGGGCGA GGAGCGACTT 
CGCTCCACCA TCGGTGTCGA TGGCTCCGTC TACAAGAAAC ATCCCCATTT TGCCAAGCGT 
CTCCATAAGG CAGTGAGGAG GCTGGTGCCC GACTGTGATG TCCGCTTCCT CCGCTCTGAG 
GATGGCAGCG GCAAGGGGGC TGCTATGGTG ACGGCGGTGG CTTACCGTCT GGCTGACCAA 
CACCGGGCCC GCCAGAAGAC CCTGGAGTCT CTGAAGCTGA GCCACGAGCA GCTTCTGGAG 
GTTAAGAGAA GAATGAAGGT GGAAATGGAG CAGGGTCTGA GCAAGGAGAC GCATGCGGTC 
GCCCCTGTGA AGATGCTGCC CACTTACGTG TGTGCCACTC CAGATGGCAC AGAGAAAGGA 
GACTTCTTGG CCTTGGATCT TGGAGGAACA AACTTCCGGG TCCTGCTGGT GCGTGTGCGT 
AATGGCAAGC GGAGGGGCGT GGAGATGCAT AACAAGATCT ACTCCATCCC ACAGGAGGTT 
ATGCATGGCA CTGGGGAAGA GCTCTTCGAC CACATTGTCC AGTGCATTGC GGACTTCCTG 
GAGTACATGG GCATGAAGGG CGTGTCCCTG CCTTTGGGTT TCACATTCTC CTTCCCTTGC 
CAGCAGAACA GCCTAGACCA GAGCATCCTC CTCAAGTGGA CAAAGGGATT CAAGGCATCT 
GGCTGCGAGG GTGAGGATGT GGTCACCTTG CTGAAGGAAG CGATTCACCG GCGAGAGGAG 
TTTGACCTGG ATGTGGTTGC CGTGGTGAAT GACACAGTTG GGACTATGAT GACTTGTGGC 
TACGAAGACC CTCACTGTGA AGTTGGCCTT ATTGTTGGCA CCGGAAGCAA CGCCTGCTAC 
ATGGAAGAGA TGCGTAATGT GGAGCTGGTG GACGGAGAGG AGGGACGGAT GTGTGTCAAC 
ATGGAGTGGG GAGCATTTGG GGACAATGGC TGCCTGGATG ACTTGCGGAC CGTGTTTGAT 
GTTGCTGTGG ATGAGCTTTC TCTCAACCCT GGCAAACAGA GGTTCGAGAA GATGATCAGC 
GGCATGTACT TGGGAGAGAT TGTGCGCAAC ATTCTCATCG ATTTCACGAA GCGGGGGCTG 
CTCTTCCGAG GCCGCATCTC AGAGCGCCTC AAGACAAGGG GAATCTTTGA AACTAAGTTC 
CTGTCTCAGA TAGAGAGCGA CTGCCTAGCC CTGCTACAGG TTCGTGCCAT CCTGCGCCAC 
CTAGGGCTGG AGAGCACGTG CGATGACAGC ATCATCGTGA AGGAGGTGTG CACTGTGGTT 
GCCCGGCGCG CTGCACAGCT CTGTGGCGCA GGCATGGCCG CCGTAGTGGA CAAGATAAGA 
GAGAACCGTG GGCTGGACAA CCTCAAAGTG ACAGTGGGCG TGGACGGGAC TCTGTATAAG 
CTTCATCCTC ACTTTGCCAA GGTCATGCAT GAGACGGTGA GAGATCTGGC TCCGAAATGT 
GACGTGTCCT TCCTGGAATC CGAGGACGGC AGTGGGAAGG GAGCAGCTCT CATCACTGCC 
GTGGCCTGCC GCATCCGGGA GGCTGGGCAG AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236773.1
CDS	1288..3723
Translation	MENQIYAIPEDIMRGSGTQLFDHIAECLANFMDKLQIKEKKLPLGFTFSFPCHQTKLDESFLVSWTKGFKSSGVEGRDVVDLIRKAIQRRGDFDIDIVAVVNDTVGTMMTCGYDDQNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGTLNDIRTEFDREIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKAELLFQGKLSPELLTTGSFETKDVSDIEEDKDGIEKAYQILMRLGLNPLQEDCVATHRICQIVSTRSASLCAATLAAVLWRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKAVRRLVPDCDVRFLRSEDGSGKGAAMVTAVAYRLADQHRARQKTLESLKLSHEQLLEVKRRMKVEMEQGLSKETHAVAPVKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKRRGVEMHNKIYSIPQEVMHGTGEELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDQSILLKWTKGFKASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGYEDPHCEVGLIVGTGSNACYMEEMRNVELVDGEEGRMCVNMEWGAFGDNGCLDDLRTVFDVAVDELSLNPGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALLQVRAILRHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAAVVDKIRENRGLDNLKVTVGVDGTLYKLHPHFAKVMHETVRDLAPKCDVSFLESEDGSGKGAALITAVACRIREAGQR

Target ORF information:

RefSeq Version	XM_006236711.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hexokinase 2 (Hk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236711.3

ORF Insert Sequence:

ATGGAGAACC AGATCTACGC CATCCCTGAG GACATCATGC GGGGCAGTGG AACCCAGCTG 
TTTGACCACA TCGCCGAATG CCTGGCCAAC TTCATGGACA AGCTACAAAT CAAAGAGAAG 
AAGCTCCCTC TGGGTTTCAC CTTCTCGTTC CCCTGCCACC AGACAAAACT GGATGAGAGT 
TTTTTGGTCT CGTGGACTAA GGGGTTCAAG TCCAGTGGCG TGGAAGGCAG AGATGTGGTG 
GACCTGATCC GGAAGGCTAT CCAGCGCAGA GGGGACTTTG ACATTGACAT TGTGGCCGTG 
GTGAATGACA CAGTTGGGAC CATGATGACT TGTGGCTATG ATGATCAGAA CTGCGAGATT 
GGTCTCATTG TGGGCACTGG CAGCAACGCC TGCTACATGG AGGAAATGCG TCATATTGAC 
ATGGTGGAGG GAGATGAGGG GCGCATGTGC ATCAACATGG AGTGGGGAGC CTTTGGGGAC 
GACGGTACAC TCAATGACAT CCGAACCGAG TTTGACCGAG AGATCGACAT GGGCTCGCTG 
AACCCTGGGA AGCAGCTGTT TGAGAAGATG ATTAGCGGGA TGTACATGGG GGAGCTGGTC 
AGGCTCATCC TGGTGAAGAT GGCCAAGGCA GAGCTGTTGT TCCAAGGGAA ACTCAGCCCA 
GAACTCCTTA CCACTGGCTC CTTCGAGACC AAAGATGTCT CGGATATTGA AGAGGATAAG 
GATGGAATCG AGAAGGCCTA CCAAATCCTG ATGCGCCTGG GTCTGAATCC ATTGCAGGAG 
GATTGTGTGG CCACGCACCG AATCTGCCAG ATTGTGTCCA CGCGCTCGGC CAGTCTGTGC 
GCAGCCACCC TGGCCGCGGT GCTGTGGCGA ATCAAAGAGA ACAAGGGCGA GGAGCGACTT 
CGCTCCACCA TCGGTGTCGA TGGCTCCGTC TACAAGAAAC ATCCCCATTT TGCCAAGCGT 
CTCCATAAGG CAGTGAGGAG GCTGGTGCCC GACTGTGATG TCCGCTTCCT CCGCTCTGAG 
GATGGCAGCG GCAAGGGGGC TGCTATGGTG ACGGCGGTGG CTTACCGTCT GGCTGACCAA 
CACCGGGCCC GCCAGAAGAC CCTGGAGTCT CTGAAGCTGA GCCACGAGCA GCTTCTGGAG 
GTTAAGAGAA GAATGAAGGT GGAAATGGAG CAGGGTCTGA GCAAGGAGAC GCATGCGGTC 
GCCCCTGTGA AGATGCTGCC CACTTACGTG TGTGCCACTC CAGATGGCAC AGAGAAAGGA 
GACTTCTTGG CCTTGGATCT TGGAGGAACA AACTTCCGGG TCCTGCTGGT GCGTGTGCGT 
AATGGCAAGC GGAGGGGCGT GGAGATGCAT AACAAGATCT ACTCCATCCC ACAGGAGGTT 
ATGCATGGCA CTGGGGAAGA GCTCTTCGAC CACATTGTCC AGTGCATTGC GGACTTCCTG 
GAGTACATGG GCATGAAGGG CGTGTCCCTG CCTTTGGGTT TCACATTCTC CTTCCCTTGC 
CAGCAGAACA GCCTAGACCA GAGCATCCTC CTCAAGTGGA CAAAGGGATT CAAGGCATCT 
GGCTGCGAGG GTGAGGATGT GGTCACCTTG CTGAAGGAAG CGATTCACCG GCGAGAGGAG 
TTTGACCTGG ATGTGGTTGC CGTGGTGAAT GACACAGTTG GGACTATGAT GACTTGTGGC 
TACGAAGACC CTCACTGTGA AGTTGGCCTT ATTGTTGGCA CCGGAAGCAA CGCCTGCTAC 
ATGGAAGAGA TGCGTAATGT GGAGCTGGTG GACGGAGAGG AGGGACGGAT GTGTGTCAAC 
ATGGAGTGGG GAGCATTTGG GGACAATGGC TGCCTGGATG ACTTGCGGAC CGTGTTTGAT 
GTTGCTGTGG ATGAGCTTTC TCTCAACCCT GGCAAACAGA GGTTCGAGAA GATGATCAGC 
GGCATGTACT TGGGAGAGAT TGTGCGCAAC ATTCTCATCG ATTTCACGAA GCGGGGGCTG 
CTCTTCCGAG GCCGCATCTC AGAGCGCCTC AAGACAAGGG GAATCTTTGA AACTAAGTTC 
CTGTCTCAGA TAGAGAGCGA CTGCCTAGCC CTGCTACAGG TTCGTGCCAT CCTGCGCCAC 
CTAGGGCTGG AGAGCACGTG CGATGACAGC ATCATCGTGA AGGAGGTGTG CACTGTGGTT 
GCCCGGCGCG CTGCACAGCT CTGTGGCGCA GGCATGGCCG CCGTAGTGGA CAAGATAAGA 
GAGAACCGTG GGCTGGACAA CCTCAAAGTG ACAGTGGGCG TGGACGGGAC TCTGTATAAG 
CTTCATCCTC ACTTTGCCAA GGTCATGCAT GAGACGGTGA GAGATCTGGC TCCGAAATGT 
GACGTGTCCT TCCTGGAATC CGAGGACGGC AGTGGGAAGG GAGCAGCTCT CATCACTGCC 
GTGGCCTGCC GCATCCGGGA GGCTGGGCAG AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hexokinase 2-dependent hyperglycolysis driving microglial activation contributes to ischemic brain injury.
Journal of neurochemistry144(2)186-200(2018 Jan)
Li Y,Lu B,Sheng L,Zhu Z,Sun H,Zhou Y,Yang Y,Xue D,Chen W,Tian X,Du Y,Yan M,Zhu W,Xing F,Li K,Lin S,Qiu P,Su X,Huang Y,Yan G,Yin W

The Catalytic Inactivation of the N-half of Human Hexokinase 2 and Structural and Biochemical Characterization of its Mitochondrial Conformation.
Bioscience reports(2018 Jan)
Nawaz MH,Ferreira JC,Nedyalkova L,Zhu H,Carrasco-L?pez C,Kirmizialtin S,Rabeh WM

Inhibition of
Bioscience reports37(4)(2017 Aug)
Zeng X,Liu N,Zhang J,Wang L,Zhang Z,Zhu J,Li Q,Wang Y

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Hk2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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