LOC100485269 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100485269
Entrez Gene ID	100485269
Full Name	hexokinase domain containing 1
General protein information	Preferred Names hexokinase domain containing 1 Names hexokinase-2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935301.5	XP_002935347.1	hexokinase-2
XM_002935301.4	XP_002935347.1	hexokinase-2

Pathways from BioSystems

Homologies

	HK2	XP_001111706.1
Caenorhabditis elegans (roundworm)	F14B4.2	NP_001021107.1
Pan troglodytes (chimpanzee)	HK2	XP_001162535.1
Gallus gallus (chicken)	HK2	NP_989543.1
Danio rerio (zebrafish)	hk2	NP_998231.1
Mus musculus (house mouse)	Hk2	NP_038848.1
Oryza sativa (rice)	Os01g0190400	NP_001042264.1
Xenopus tropicalis (tropical clawed frog)	LOC100485269	XP_002935347.1
Homo sapiens (human)	HK2	NP_000180.2
Rattus norvegicus (Norway rat)	Hk2	NP_036867.1
Canis lupus familiaris (dog)	HK2	XP_005630567.1
Bos taurus (cattle)	HK2	XP_002691235.1

Gene Ontology

The following LOC100485269 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100485269 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa07164	XM_002935301.4 Latest version!	Xenopus tropicalis hexokinase-2 (LOC100485269), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa55287	XM_002935301.5 Latest version!	Xenopus tropicalis hexokinase domain containing 1 (hkdc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100485269 ( XM_002935301.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935347.1 Xenopus tropicalis hexokinase-2 (LOC100485269), mRNA.

Price & Availability↑

CloneID	OXa07164
Clone ID Related Accession (Same CDS sequence)	XM_002935301.4
Accession Version	XM_002935301.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hexokinase-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935301.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGCAT CCCACTTGCT GGCATATTTC TTCACTGAGC TCAACCATGA CCAAGTTCAG 
AAGGTGGACA AGTACCTATA CCACATGCGC CTATCAGATG AGACACTCTT GGAAGTGTCA 
AGCCGATTTG AGAAGCAGAT GGAGAAAGGA CTTGGGCAAG AGACCAGCCC CACCGCCTGC 
GTGAAGATGC TGCCCACATT TGTACGATCT ACCCCAGATG GGACAGAAAA TGGAGACTTT 
TTAGCCCTGG ACCTTGGAGG GACGAATTTC CGTGTGCTTC GTGTTAAAGT GTCTGATAAT 
GGAATGAAGA AAGTGGAGAT GGAGAACCAG ATATATGCTA TCCCAGCAGA TCTTATGCGT 
GGCAGCGGGG AACAGCTCTT TGACCACATT GCAGAATGCC TGGGCAATTT CATGGAGAAG 
CTGAACATCA AAGACAAGAA ACTGCCTTTA GGATTCACTT TTTCCTTCCC CTGCCAACAG 
ACCAAACTGG ATGAGAGTAT TCTAGTGAAC TGGACAAAGG GATTTAAAGC CTGTGGAGTT 
GAAGGAAAAG ATGTGGTCAC CCTTTTACGG AAGGCAATCA AGAAAAGAGG AGATTTTGAC 
ATTGATATTG TAGCAGTGGT GAATGATACT GTAGGAACCA TGATGACTTG TGGATATGAT 
GACCACAACT GTGAGATTGG ACTCATTGTT GGTACAGGGA CCAATGCATG TTACATGGAA 
GACATGCGCC ACATCGATTT AGTGGAGGGC GATGAGGGAA GAATGTGCAT CAACATGGAG 
TGGGGAGCTT TTGGGGATGA TGGCTGTTTG GACGAAATTA GGACACAGTT TGATAGGGAA 
ATCGACATGG GATCTTTAAA CCCAGGGAAA CAATTGTTTG AGAAGATGAT CAGCGGAATG 
TATATGGGGG AGCTGGTCAG GTTTATCCTT GTAAAAATGG CCAAAGAAGA CTTGTTGTTC 
GAAGGCAGAA TCACAGCTGA TCTCCTGACC CGTGGCCACT TTGAAACCCG ATATGTTTCA 
GAAATTGAGA AGGACAAGGA AGGGATCAAG AAAGCACATG AGATCCTTAC CAAGCTTGGC 
TTGGAGCCCT CACATGAGGA TTCTGTTGCC ACACACCGCA TTTGTCAGAT TGTGTCAACC 
CGTTCTGCCA ATCTGTGTGC AGCCACGCTC GGTGCCGTAC TAAGGAGAAT CCGGGAAAAC 
AAGGGTGTGG AAAGGCTAAA ATCTACAGTG GGAGTTGATG GATCTGTCTA CAAAAACCAT 
CCTCACTTTG CCAGACGTCT TCAGAAGACT GTCCGCCGCT TGGTGCCAGA ATGTGATGTT 
CGATTCATTC GGTCAGAGGA TGGAAGTGGC AAAGGTGCAG CTATGGTGAC TGCTGTAGCC 
TACAGACTGG CTGACCAGCA GCATGAGCGG GAGAAGACAC TGGAAGCCTT CAGTCTTAGC 
ACAAAGCAGC TCCTGGAAGT CAAGAAGATA ATGAGGCAGG AGATGGACAG AGGACTGAAA 
AAGGAAAGTC ATGAAGAGGC TCCAGTTAAA ATGTTGCCCA CATATGTGCG CTCAACCCCT 
GATGGCACAG AGAAGGGAGA TTTCCTGGCA CTAGATCTTG GAGGCACCAA TTTCCGTGTA 
CTACTTGTTC GTGTTCGGAA TGGGAGACGA GGAGTGGAAA TGCACAATAA AATCTACACA 
ATTCCCCAGG ATGCAATGGT GGGAACAGGT GAAGAGCTGT TTGACCACAT TGTTCATTGT 
ATTGCGGATT TTCTGGAGTA CATGGGCATG AAAGGAGTCT CCCTTCCCCT TGGATTCACC 
TTTTCTTTTC CTTGCCACCA AAACAGTTTG AATGAGGGAA TTCTACTCAA GTGGACAAAA 
GGCTTTAAGG CAACTGGATG TGAAGGAGAG GATGTTGTAA ATCTGTTGAA GGAAGCTATC 
CATAGGCACG AGGAATTTGA TCTGGATGTA GTAGCTGTTG TAAATGACAC AGTGGGCACC 
ATGATGACCT GTGGCTACGA GGACCCTAAC TGTGAGATGG GGCTTATTGT AGGTACTGGC 
AGCAATGCCT GTTACATGGA GGAGATGAGA AACGTGGAGC TGGTTGAAGG GGAGGAGGGC 
AGAATGTGCA TTAATATGGA GTGGGGAGCA TTCGGGGACA ATGACTGCCT GGAGGAGTTC 
CGCACAGTTT TCGACAGAGC TGTGGATGAA CTCTCCCTCA ATCCCGGAAA GCAGAGGTAT 
GAGAAGATGA TCAGTGGCAT GTACTTGGGG GAGATCGTCA GAAACATCCT GATAGATTTC 
ACCAAACGGG GGCTGCTTTT CCGAGGGAAG ATTTCAGAGA GGCTCAAGAC CAGGGGCATC 
TTTGAAACAA AGTTCTTATC ACAGATAGAG AGTGATCGCC TGGCTCTGCT TCAGGTCCGC 
TCCATCCTAC AGCACCTTGG TTTAAACAGC ACCTGTGATG ACAGTATCAT TGTAAAAGAA 
GTATGCACAG TAGTGGCAAG GAGAGCTGCT CAGCTGTGTG GAGCTGGAGT GGCAGCAGTG 
GTGGATAAGA TCAGGGAGAA TCGAGGCCTG GAGACCCTAA AGGTCACAGT TGGAGTGGAT 
GGGACTCTCT ACAAACTTCA CCCACACTTT TCTTCCATCA TGCACCAGAC CGTGAATGAT 
CTATCCCCAA AATGCCAAGT GACATTTTTA CAGTCAGAAG ATGGCAGTGG AAAGGGAGCT 
GCTTTGATCA CTGCGGTGGC ATGCAGAATC CGGGAAGCGG GAGAGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935347.1
CDS	395..3145
Translation	MIASHLLAYFFTELNHDQVQKVDKYLYHMRLSDETLLEVSSRFEKQMEKGLGQETSPTACVKMLPTFVRSTPDGTENGDFLALDLGGTNFRVLRVKVSDNGMKKVEMENQIYAIPADLMRGSGEQLFDHIAECLGNFMEKLNIKDKKLPLGFTFSFPCQQTKLDESILVNWTKGFKACGVEGKDVVTLLRKAIKKRGDFDIDIVAVVNDTVGTMMTCGYDDHNCEIGLIVGTGTNACYMEDMRHIDLVEGDEGRMCINMEWGAFGDDGCLDEIRTQFDREIDMGSLNPGKQLFEKMISGMYMGELVRFILVKMAKEDLLFEGRITADLLTRGHFETRYVSEIEKDKEGIKKAHEILTKLGLEPSHEDSVATHRICQIVSTRSANLCAATLGAVLRRIRENKGVERLKSTVGVDGSVYKNHPHFARRLQKTVRRLVPECDVRFIRSEDGSGKGAAMVTAVAYRLADQQHEREKTLEAFSLSTKQLLEVKKIMRQEMDRGLKKESHEEAPVKMLPTYVRSTPDGTEKGDFLALDLGGTNFRVLLVRVRNGRRGVEMHNKIYTIPQDAMVGTGEELFDHIVHCIADFLEYMGMKGVSLPLGFTFSFPCHQNSLNEGILLKWTKGFKATGCEGEDVVNLLKEAIHRHEEFDLDVVAVVNDTVGTMMTCGYEDPNCEMGLIVGTGSNACYMEEMRNVELVEGEEGRMCINMEWGAFGDNDCLEEFRTVFDRAVDELSLNPGKQRYEKMISGMYLGEIVRNILIDFTKRGLLFRGKISERLKTRGIFETKFLSQIESDRLALLQVRSILQHLGLNSTCDDSIIVKEVCTVVARRAAQLCGAGVAAVVDKIRENRGLETLKVTVGVDGTLYKLHPHFSSIMHQTVNDLSPKCQVTFLQSEDGSGKGAALITAVACRIREAGER

Target ORF information:

RefSeq Version	XM_002935301.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis hexokinase-2 (LOC100485269), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935301.4

ORF Insert Sequence:

ATGATAGCAT CCCACTTGCT GGCATATTTC TTCACTGAGC TCAACCATGA CCAAGTTCAG 
AAGGTGGACA AGTACCTATA CCACATGCGC CTATCAGATG AGACACTCTT GGAAGTGTCA 
AGCCGATTTG AGAAGCAGAT GGAGAAAGGA CTTGGGCAAG AGACCAGCCC CACCGCCTGC 
GTGAAGATGC TGCCCACATT TGTACGATCT ACCCCAGATG GGACAGAAAA TGGAGACTTT 
TTAGCCCTGG ACCTTGGAGG GACGAATTTC CGTGTGCTTC GTGTTAAAGT GTCTGATAAT 
GGAATGAAGA AAGTGGAGAT GGAGAACCAG ATATATGCTA TCCCAGCAGA TCTTATGCGT 
GGCAGCGGGG AACAGCTCTT TGACCACATT GCAGAATGCC TGGGCAATTT CATGGAGAAG 
CTGAACATCA AAGACAAGAA ACTGCCTTTA GGATTCACTT TTTCCTTCCC CTGCCAACAG 
ACCAAACTGG ATGAGAGTAT TCTAGTGAAC TGGACAAAGG GATTTAAAGC CTGTGGAGTT 
GAAGGAAAAG ATGTGGTCAC CCTTTTACGG AAGGCAATCA AGAAAAGAGG AGATTTTGAC 
ATTGATATTG TAGCAGTGGT GAATGATACT GTAGGAACCA TGATGACTTG TGGATATGAT 
GACCACAACT GTGAGATTGG ACTCATTGTT GGTACAGGGA CCAATGCATG TTACATGGAA 
GACATGCGCC ACATCGATTT AGTGGAGGGC GATGAGGGAA GAATGTGCAT CAACATGGAG 
TGGGGAGCTT TTGGGGATGA TGGCTGTTTG GACGAAATTA GGACACAGTT TGATAGGGAA 
ATCGACATGG GATCTTTAAA CCCAGGGAAA CAATTGTTTG AGAAGATGAT CAGCGGAATG 
TATATGGGGG AGCTGGTCAG GTTTATCCTT GTAAAAATGG CCAAAGAAGA CTTGTTGTTC 
GAAGGCAGAA TCACAGCTGA TCTCCTGACC CGTGGCCACT TTGAAACCCG ATATGTTTCA 
GAAATTGAGA AGGACAAGGA AGGGATCAAG AAAGCACATG AGATCCTTAC CAAGCTTGGC 
TTGGAGCCCT CACATGAGGA TTCTGTTGCC ACACACCGCA TTTGTCAGAT TGTGTCAACC 
CGTTCTGCCA ATCTGTGTGC AGCCACGCTC GGTGCCGTAC TAAGGAGAAT CCGGGAAAAC 
AAGGGTGTGG AAAGGCTAAA ATCTACAGTG GGAGTTGATG GATCTGTCTA CAAAAACCAT 
CCTCACTTTG CCAGACGTCT TCAGAAGACT GTCCGCCGCT TGGTGCCAGA ATGTGATGTT 
CGATTCATTC GGTCAGAGGA TGGAAGTGGC AAAGGTGCAG CTATGGTGAC TGCTGTAGCC 
TACAGACTGG CTGACCAGCA GCATGAGCGG GAGAAGACAC TGGAAGCCTT CAGTCTTAGC 
ACAAAGCAGC TCCTGGAAGT CAAGAAGATA ATGAGGCAGG AGATGGACAG AGGACTGAAA 
AAGGAAAGTC ATGAAGAGGC TCCAGTTAAA ATGTTGCCCA CATATGTGCG CTCAACCCCT 
GATGGCACAG AGAAGGGAGA TTTCCTGGCA CTAGATCTTG GAGGCACCAA TTTCCGTGTA 
CTACTTGTTC GTGTTCGGAA TGGGAGACGA GGAGTGGAAA TGCACAATAA AATCTACACA 
ATTCCCCAGG ATGCAATGGT GGGAACAGGT GAAGAGCTGT TTGACCACAT TGTTCATTGT 
ATTGCGGATT TTCTGGAGTA CATGGGCATG AAAGGAGTCT CCCTTCCCCT TGGATTCACC 
TTTTCTTTTC CTTGCCACCA AAACAGTTTG AATGAGGGAA TTCTACTCAA GTGGACAAAA 
GGCTTTAAGG CAACTGGATG TGAAGGAGAG GATGTTGTAA ATCTGTTGAA GGAAGCTATC 
CATAGGCACG AGGAATTTGA TCTGGATGTA GTAGCTGTTG TAAATGACAC AGTGGGCACC 
ATGATGACCT GTGGCTACGA GGACCCTAAC TGTGAGATGG GGCTTATTGT AGGTACTGGC 
AGCAATGCCT GTTACATGGA GGAGATGAGA AACGTGGAGC TGGTTGAAGG GGAGGAGGGC 
AGAATGTGCA TTAATATGGA GTGGGGAGCA TTCGGGGACA ATGACTGCCT GGAGGAGTTC 
CGCACAGTTT TCGACAGAGC TGTGGATGAA CTCTCCCTCA ATCCCGGAAA GCAGAGGTAT 
GAGAAGATGA TCAGTGGCAT GTACTTGGGG GAGATCGTCA GAAACATCCT GATAGATTTC 
ACCAAACGGG GGCTGCTTTT CCGAGGGAAG ATTTCAGAGA GGCTCAAGAC CAGGGGCATC 
TTTGAAACAA AGTTCTTATC ACAGATAGAG AGTGATCGCC TGGCTCTGCT TCAGGTCCGC 
TCCATCCTAC AGCACCTTGG TTTAAACAGC ACCTGTGATG ACAGTATCAT TGTAAAAGAA 
GTATGCACAG TAGTGGCAAG GAGAGCTGCT CAGCTGTGTG GAGCTGGAGT GGCAGCAGTG 
GTGGATAAGA TCAGGGAGAA TCGAGGCCTG GAGACCCTAA AGGTCACAGT TGGAGTGGAT 
GGGACTCTCT ACAAACTTCA CCCACACTTT TCTTCCATCA TGCACCAGAC CGTGAATGAT 
CTATCCCCAA AATGCCAAGT GACATTTTTA CAGTCAGAAG ATGGCAGTGG AAAGGGAGCT 
GCTTTGATCA CTGCGGTGGC ATGCAGAATC CGGGAAGCGG GAGAGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100485269 ( XM_002935301.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935347.1 Xenopus tropicalis hexokinase domain containing 1 (hkdc1), mRNA.

Price & Availability↑

CloneID	OXa55287
Clone ID Related Accession (Same CDS sequence)	XM_002935301.5
Accession Version	XM_002935301.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hexokinase-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935301.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGCAT CCCACTTGCT GGCATATTTC TTCACTGAGC TCAACCATGA CCAAGTTCAG 
AAGGTGGACA AGTACCTATA CCACATGCGC CTATCAGATG AGACACTCTT GGAAGTGTCA 
AGCCGATTTG AGAAGCAGAT GGAGAAAGGA CTTGGGCAAG AGACCAGCCC CACCGCCTGC 
GTGAAGATGC TGCCCACATT TGTACGATCT ACCCCAGATG GGACAGAAAA TGGAGACTTT 
TTAGCCCTGG ACCTTGGAGG GACGAATTTC CGTGTGCTTC GTGTTAAAGT GTCTGATAAT 
GGAATGAAGA AAGTGGAGAT GGAGAACCAG ATATATGCTA TCCCAGCAGA TCTTATGCGT 
GGCAGCGGGG AACAGCTCTT TGACCACATT GCAGAATGCC TGGGCAATTT CATGGAGAAG 
CTGAACATCA AAGACAAGAA ACTGCCTTTA GGATTCACTT TTTCCTTCCC CTGCCAACAG 
ACCAAACTGG ATGAGAGTAT TCTAGTGAAC TGGACAAAGG GATTTAAAGC CTGTGGAGTT 
GAAGGAAAAG ATGTGGTCAC CCTTTTACGG AAGGCAATCA AGAAAAGAGG AGATTTTGAC 
ATTGATATTG TAGCAGTGGT GAATGATACT GTAGGAACCA TGATGACTTG TGGATATGAT 
GACCACAACT GTGAGATTGG ACTCATTGTT GGTACAGGGA CCAATGCATG TTACATGGAA 
GACATGCGCC ACATCGATTT AGTGGAGGGC GATGAGGGAA GAATGTGCAT CAACATGGAG 
TGGGGAGCTT TTGGGGATGA TGGCTGTTTG GACGAAATTA GGACACAGTT TGATAGGGAA 
ATCGACATGG GATCTTTAAA CCCAGGGAAA CAATTGTTTG AGAAGATGAT CAGCGGAATG 
TATATGGGGG AGCTGGTCAG GTTTATCCTT GTAAAAATGG CCAAAGAAGA CTTGTTGTTC 
GAAGGCAGAA TCACAGCTGA TCTCCTGACC CGTGGCCACT TTGAAACCCG ATATGTTTCA 
GAAATTGAGA AGGACAAGGA AGGGATCAAG AAAGCACATG AGATCCTTAC CAAGCTTGGC 
TTGGAGCCCT CACATGAGGA TTCTGTTGCC ACACACCGCA TTTGTCAGAT TGTGTCAACC 
CGTTCTGCCA ATCTGTGTGC AGCCACGCTC GGTGCCGTAC TAAGGAGAAT CCGGGAAAAC 
AAGGGTGTGG AAAGGCTAAA ATCTACAGTG GGAGTTGATG GATCTGTCTA CAAAAACCAT 
CCTCACTTTG CCAGACGTCT TCAGAAGACT GTCCGCCGCT TGGTGCCAGA ATGTGATGTT 
CGATTCATTC GGTCAGAGGA TGGAAGTGGC AAAGGTGCAG CTATGGTGAC TGCTGTAGCC 
TACAGACTGG CTGACCAGCA GCATGAGCGG GAGAAGACAC TGGAAGCCTT CAGTCTTAGC 
ACAAAGCAGC TCCTGGAAGT CAAGAAGATA ATGAGGCAGG AGATGGACAG AGGACTGAAA 
AAGGAAAGTC ATGAAGAGGC TCCAGTTAAA ATGTTGCCCA CATATGTGCG CTCAACCCCT 
GATGGCACAG AGAAGGGAGA TTTCCTGGCA CTAGATCTTG GAGGCACCAA TTTCCGTGTA 
CTACTTGTTC GTGTTCGGAA TGGGAGACGA GGAGTGGAAA TGCACAATAA AATCTACACA 
ATTCCCCAGG ATGCAATGGT GGGAACAGGT GAAGAGCTGT TTGACCACAT TGTTCATTGT 
ATTGCGGATT TTCTGGAGTA CATGGGCATG AAAGGAGTCT CCCTTCCCCT TGGATTCACC 
TTTTCTTTTC CTTGCCACCA AAACAGTTTG AATGAGGGAA TTCTACTCAA GTGGACAAAA 
GGCTTTAAGG CAACTGGATG TGAAGGAGAG GATGTTGTAA ATCTGTTGAA GGAAGCCATC 
CATAGGCACG AGGAATTTGA TCTGGATGTA GTAGCTGTTG TAAATGACAC AGTGGGCACC 
ATGATGACCT GTGGCTACGA GGACCCTAAC TGTGAGATGG GGCTTATTGT AGGTACTGGC 
AGCAATGCCT GTTACATGGA GGAGATGAGA AACGTGGAGC TGGTTGAAGG GGAGGAGGGC 
AGAATGTGCA TTAATATGGA GTGGGGAGCA TTCGGGGACA ATGACTGCCT GGAGGAGTTT 
CGCACAGTTT TCGACAGAGC TGTGGATGAA CTCTCCCTCA ATCCCGGAAA GCAGAGGTAT 
GAGAAGATGA TCAGTGGCAT GTACTTGGGG GAGATCGTCA GAAACATCCT GATAGATTTC 
ACCAAACGGG GGCTGCTTTT CCGAGGGAAG ATTTCAGAGA GGCTCAAGAC CAGGGGCATC 
TTTGAAACAA AGTTCTTATC ACAGATAGAG AGTGATCGCC TGGCTCTGCT TCAGGTCCGC 
TCCATCCTAC AGCACCTTGG TTTAAACAGC ACCTGTGATG ACAGTATCAT TGTAAAAGAA 
GTATGCACAG TAGTGGCAAG GAGAGCTGCT CAGCTGTGTG GAGCTGGAGT GGCAGCAGTG 
GTGGATAAGA TCAGGGAGAA TCGAGGCCTG GAGACCCTAA AGGTCACAGT TGGAGTGGAT 
GGGACTCTCT ACAAACTTCA CCCACACTTT TCTTCCATCA TGCACCAGAC CGTGAATGAT 
CTATCCCCAA AATGCCAAGT GACATTTTTA CAGTCAGAAG ATGGCAGTGG AAAGGGAGCT 
GCTTTGATCA CTGCGGTGGC ATGCAGAATC CGGGAAGCGG GAGAGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935347.1
CDS	362..3112
Translation	MIASHLLAYFFTELNHDQVQKVDKYLYHMRLSDETLLEVSSRFEKQMEKGLGQETSPTACVKMLPTFVRSTPDGTENGDFLALDLGGTNFRVLRVKVSDNGMKKVEMENQIYAIPADLMRGSGEQLFDHIAECLGNFMEKLNIKDKKLPLGFTFSFPCQQTKLDESILVNWTKGFKACGVEGKDVVTLLRKAIKKRGDFDIDIVAVVNDTVGTMMTCGYDDHNCEIGLIVGTGTNACYMEDMRHIDLVEGDEGRMCINMEWGAFGDDGCLDEIRTQFDREIDMGSLNPGKQLFEKMISGMYMGELVRFILVKMAKEDLLFEGRITADLLTRGHFETRYVSEIEKDKEGIKKAHEILTKLGLEPSHEDSVATHRICQIVSTRSANLCAATLGAVLRRIRENKGVERLKSTVGVDGSVYKNHPHFARRLQKTVRRLVPECDVRFIRSEDGSGKGAAMVTAVAYRLADQQHEREKTLEAFSLSTKQLLEVKKIMRQEMDRGLKKESHEEAPVKMLPTYVRSTPDGTEKGDFLALDLGGTNFRVLLVRVRNGRRGVEMHNKIYTIPQDAMVGTGEELFDHIVHCIADFLEYMGMKGVSLPLGFTFSFPCHQNSLNEGILLKWTKGFKATGCEGEDVVNLLKEAIHRHEEFDLDVVAVVNDTVGTMMTCGYEDPNCEMGLIVGTGSNACYMEEMRNVELVEGEEGRMCINMEWGAFGDNDCLEEFRTVFDRAVDELSLNPGKQRYEKMISGMYLGEIVRNILIDFTKRGLLFRGKISERLKTRGIFETKFLSQIESDRLALLQVRSILQHLGLNSTCDDSIIVKEVCTVVARRAAQLCGAGVAAVVDKIRENRGLETLKVTVGVDGTLYKLHPHFSSIMHQTVNDLSPKCQVTFLQSEDGSGKGAALITAVACRIREAGER

Target ORF information:

RefSeq Version	XM_002935301.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis hexokinase domain containing 1 (hkdc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935301.5

ORF Insert Sequence:

ATGATAGCAT CCCACTTGCT GGCATATTTC TTCACTGAGC TCAACCATGA CCAAGTTCAG 
AAGGTGGACA AGTACCTATA CCACATGCGC CTATCAGATG AGACACTCTT GGAAGTGTCA 
AGCCGATTTG AGAAGCAGAT GGAGAAAGGA CTTGGGCAAG AGACCAGCCC CACCGCCTGC 
GTGAAGATGC TGCCCACATT TGTACGATCT ACCCCAGATG GGACAGAAAA TGGAGACTTT 
TTAGCCCTGG ACCTTGGAGG GACGAATTTC CGTGTGCTTC GTGTTAAAGT GTCTGATAAT 
GGAATGAAGA AAGTGGAGAT GGAGAACCAG ATATATGCTA TCCCAGCAGA TCTTATGCGT 
GGCAGCGGGG AACAGCTCTT TGACCACATT GCAGAATGCC TGGGCAATTT CATGGAGAAG 
CTGAACATCA AAGACAAGAA ACTGCCTTTA GGATTCACTT TTTCCTTCCC CTGCCAACAG 
ACCAAACTGG ATGAGAGTAT TCTAGTGAAC TGGACAAAGG GATTTAAAGC CTGTGGAGTT 
GAAGGAAAAG ATGTGGTCAC CCTTTTACGG AAGGCAATCA AGAAAAGAGG AGATTTTGAC 
ATTGATATTG TAGCAGTGGT GAATGATACT GTAGGAACCA TGATGACTTG TGGATATGAT 
GACCACAACT GTGAGATTGG ACTCATTGTT GGTACAGGGA CCAATGCATG TTACATGGAA 
GACATGCGCC ACATCGATTT AGTGGAGGGC GATGAGGGAA GAATGTGCAT CAACATGGAG 
TGGGGAGCTT TTGGGGATGA TGGCTGTTTG GACGAAATTA GGACACAGTT TGATAGGGAA 
ATCGACATGG GATCTTTAAA CCCAGGGAAA CAATTGTTTG AGAAGATGAT CAGCGGAATG 
TATATGGGGG AGCTGGTCAG GTTTATCCTT GTAAAAATGG CCAAAGAAGA CTTGTTGTTC 
GAAGGCAGAA TCACAGCTGA TCTCCTGACC CGTGGCCACT TTGAAACCCG ATATGTTTCA 
GAAATTGAGA AGGACAAGGA AGGGATCAAG AAAGCACATG AGATCCTTAC CAAGCTTGGC 
TTGGAGCCCT CACATGAGGA TTCTGTTGCC ACACACCGCA TTTGTCAGAT TGTGTCAACC 
CGTTCTGCCA ATCTGTGTGC AGCCACGCTC GGTGCCGTAC TAAGGAGAAT CCGGGAAAAC 
AAGGGTGTGG AAAGGCTAAA ATCTACAGTG GGAGTTGATG GATCTGTCTA CAAAAACCAT 
CCTCACTTTG CCAGACGTCT TCAGAAGACT GTCCGCCGCT TGGTGCCAGA ATGTGATGTT 
CGATTCATTC GGTCAGAGGA TGGAAGTGGC AAAGGTGCAG CTATGGTGAC TGCTGTAGCC 
TACAGACTGG CTGACCAGCA GCATGAGCGG GAGAAGACAC TGGAAGCCTT CAGTCTTAGC 
ACAAAGCAGC TCCTGGAAGT CAAGAAGATA ATGAGGCAGG AGATGGACAG AGGACTGAAA 
AAGGAAAGTC ATGAAGAGGC TCCAGTTAAA ATGTTGCCCA CATATGTGCG CTCAACCCCT 
GATGGCACAG AGAAGGGAGA TTTCCTGGCA CTAGATCTTG GAGGCACCAA TTTCCGTGTA 
CTACTTGTTC GTGTTCGGAA TGGGAGACGA GGAGTGGAAA TGCACAATAA AATCTACACA 
ATTCCCCAGG ATGCAATGGT GGGAACAGGT GAAGAGCTGT TTGACCACAT TGTTCATTGT 
ATTGCGGATT TTCTGGAGTA CATGGGCATG AAAGGAGTCT CCCTTCCCCT TGGATTCACC 
TTTTCTTTTC CTTGCCACCA AAACAGTTTG AATGAGGGAA TTCTACTCAA GTGGACAAAA 
GGCTTTAAGG CAACTGGATG TGAAGGAGAG GATGTTGTAA ATCTGTTGAA GGAAGCCATC 
CATAGGCACG AGGAATTTGA TCTGGATGTA GTAGCTGTTG TAAATGACAC AGTGGGCACC 
ATGATGACCT GTGGCTACGA GGACCCTAAC TGTGAGATGG GGCTTATTGT AGGTACTGGC 
AGCAATGCCT GTTACATGGA GGAGATGAGA AACGTGGAGC TGGTTGAAGG GGAGGAGGGC 
AGAATGTGCA TTAATATGGA GTGGGGAGCA TTCGGGGACA ATGACTGCCT GGAGGAGTTT 
CGCACAGTTT TCGACAGAGC TGTGGATGAA CTCTCCCTCA ATCCCGGAAA GCAGAGGTAT 
GAGAAGATGA TCAGTGGCAT GTACTTGGGG GAGATCGTCA GAAACATCCT GATAGATTTC 
ACCAAACGGG GGCTGCTTTT CCGAGGGAAG ATTTCAGAGA GGCTCAAGAC CAGGGGCATC 
TTTGAAACAA AGTTCTTATC ACAGATAGAG AGTGATCGCC TGGCTCTGCT TCAGGTCCGC 
TCCATCCTAC AGCACCTTGG TTTAAACAGC ACCTGTGATG ACAGTATCAT TGTAAAAGAA 
GTATGCACAG TAGTGGCAAG GAGAGCTGCT CAGCTGTGTG GAGCTGGAGT GGCAGCAGTG 
GTGGATAAGA TCAGGGAGAA TCGAGGCCTG GAGACCCTAA AGGTCACAGT TGGAGTGGAT 
GGGACTCTCT ACAAACTTCA CCCACACTTT TCTTCCATCA TGCACCAGAC CGTGAATGAT 
CTATCCCCAA AATGCCAAGT GACATTTTTA CAGTCAGAAG ATGGCAGTGG AAAGGGAGCT 
GCTTTGATCA CTGCGGTGGC ATGCAGAATC CGGGAAGCGG GAGAGCGCTA G