Hint3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hint3
Entrez Gene ID	246769
Full Name	histidine triad nucleotide binding protein 3
Synonyms	HINT-3,HINT-4,Hint4
General protein information	Preferred Names histidine triad nucleotide binding protein 3 Names histidine triad nucleotide-binding protein 3 histidine triad nucleotide binding protein 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p11
Summary	member of the histidine triad protein family [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276433.1	NP_001263362.1	histidine triad nucleotide-binding protein 3 isoform 2
NM_001100825.1	NP_001094295.1	histidine triad nucleotide-binding protein 3 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HINT3	XP_001165850.1
Canis lupus familiaris (dog)	HINT3	XP_533489.2
	HINT3	XP_001107408.1
Mus musculus (house mouse)	Hint3	NP_080074.1
Gallus gallus (chicken)	HINT3	XP_001232866.1
Danio rerio (zebrafish)	hint3	XP_001334761.2
Xenopus tropicalis (tropical clawed frog)	hint3	NP_001039146.1
Drosophila melanogaster (fruit fly)	CG15362	NP_608634.1
Homo sapiens (human)	HINT3	NP_612638.3
Bos taurus (cattle)	HINT3	NP_776766.1
Rattus norvegicus (Norway rat)	Hint3	NP_001094295.1
Drosophila melanogaster (fruit fly)	CG34015	NP_001033848.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007577	XP_308297.3

Gene Ontology
Bibliography

Related articles in PubMed

Evidence that human histidine triad nucleotide binding protein 3 (Hint3) is a distinct branch of the histidine triad (HIT) superfamily.
Chou TF, Cheng J, Tikh IB, Wagner CR
Journal of molecular biology373(4)978-89(2007 Nov)

Hint, Fhit, and GalT: function, structure, evolution, and mechanism of three branches of the histidine triad superfamily of nucleotide hydrolases and transferases.
Brenner C
Biochemistry41(29)9003-14(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hint3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hint3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10688	NM_001100825.1 Latest version!	Rattus norvegicus histidine triad nucleotide binding protein 3 (Hint3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa11380	NM_001276433.1 Latest version!	Rattus norvegicus histidine triad nucleotide binding protein 3 (Hint3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hint3 ( NM_001100825.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094295.1 Rattus norvegicus histidine triad nucleotide binding protein 3 (Hint3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa10688
Clone ID Related Accession (Same CDS sequence)	NM_001100825.1
Accession Version	NM_001100825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	histidine triad nucleotide-binding protein 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07000944.1 and CB741212.1. On or before Feb 16, 2013 this sequence version replaced XM_341742.3, XM_001055373.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FQ215151.1, CB584964.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAA AGCAAGCGGG CTTAGCTGGA GAGCCGAATC CCGACTGTAC GGTCACTGCC 
AAAGCAGGAC CTGAGGTGTC CTCGCCTGGG ACCTCGGAGT CCAGGGACTA TGACAGTAAC 
TGCGTGTTCT GCCGCGTCGC GGCGGGTCAG GAACCCGAAA CTGAACTCTT ATACTGTGAG 
AACAAAGACC TGGTTTGTTT CAAAGATATC AAACCAGCAG CACTTCACCA TTACCTCGTG 
GTGCCAAAAA AGCATATTGG AAGTTGCAAG GATCTAAACA AAGATCACAT AGAAATGGTT 
GAGAGCATGG TCACTGTTGG AAAAACCATT CTTGAGAGGA ATAATTTCAC AGACTTCACA 
GATGTGAGAA TGGGCTTCCA TGTGCCTCCG TTCTGCTCCG TCTCTCATCT GCACCTTCAT 
GTCATAGCGC CCGCCAAAGA GTTCGGCTTC CTATCGAGGG TGGTTTACAG GCGGGATTCC 
TACTGGTTTA TCACGGTTGA TTATTTGCTT GAAAAGCTAA GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094295.1
CDS	31..558
Translation	MAEKQAGLAGEPNPDCTVTAKAGPEVSSPGTSESRDYDSNCVFCRVAAGQEPETELLYCENKDLVCFKDIKPAALHHYLVVPKKHIGSCKDLNKDHIEMVESMVTVGKTILERNNFTDFTDVRMGFHVPPFCSVSHLHLHVIAPAKEFGFLSRVVYRRDSYWFITVDYLLEKLRK

Target ORF information:

RefSeq Version	NM_001100825.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus histidine triad nucleotide binding protein 3 (Hint3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100825.1

ORF Insert Sequence:

ATGGCTGAAA AGCAAGCGGG CTTAGCTGGA GAGCCGAATC CCGACTGTAC GGTCACTGCC 
AAAGCAGGAC CTGAGGTGTC CTCGCCTGGG ACCTCGGAGT CCAGGGACTA TGACAGTAAC 
TGCGTGTTCT GCCGCGTCGC GGCGGGTCAG GAACCCGAAA CTGAACTCTT ATACTGTGAG 
AACAAAGACC TGGTTTGTTT CAAAGATATC AAACCAGCAG CACTTCACCA TTACCTCGTG 
GTGCCAAAAA AGCATATTGG AAGTTGCAAG GATCTAAACA AAGATCACAT AGAAATGGTT 
GAGAGCATGG TCACTGTTGG AAAAACCATT CTTGAGAGGA ATAATTTCAC AGACTTCACA 
GATGTGAGAA TGGGCTTCCA TGTGCCTCCG TTCTGCTCCG TCTCTCATCT GCACCTTCAT 
GTCATAGCGC CCGCCAAAGA GTTCGGCTTC CTATCGAGGG TGGTTTACAG GCGGGATTCC 
TACTGGTTTA TCACGGTTGA TTATTTGCTT GAAAAGCTAA GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hint3 ( NM_001276433.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001263362.1 Rattus norvegicus histidine triad nucleotide binding protein 3 (Hint3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa11380
Clone ID Related Accession (Same CDS sequence)	NM_001276433.1
Accession Version	NM_001276433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	histidine triad nucleotide-binding protein 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07000944.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CF108829.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAA AGCAAGCGGG CTTAGCTGGA GAGCCGAATC CCGACTGTAC GGTCACTGCC 
AAAGCAGGAC CTGAGGTGTC CTCGCCTGGG ACCTCGGAGT CCAGGGACTA TGACAGTAAC 
TGCGTGTTCT GCCGCGTCGC GGCGGGTCAG GAACCCGAAA CTGAACTCTT ATACTGTGAG 
AACAAAGACC TGGTTTGTTT CAAAGATATC AAACCAGCAG CACTTCACCA TTACCTCGTG 
GTGCCAAAAA AGCATATTGG AAGTTGCAAG GATCTAAACA AAGATCACAT AGAAATGGTT 
GAGAGCATGG TCACTGTTGG AAAAACCATT CTTGAGAGGA ATAATTTCAC AGACTTCACA 
GATGTGAGGT TGATTATTTG CTTGAAAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263362.1
CDS	31..423
Translation	MAEKQAGLAGEPNPDCTVTAKAGPEVSSPGTSESRDYDSNCVFCRVAAGQEPETELLYCENKDLVCFKDIKPAALHHYLVVPKKHIGSCKDLNKDHIEMVESMVTVGKTILERNNFTDFTDVRLIICLKS

Target ORF information:

RefSeq Version	NM_001276433.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus histidine triad nucleotide binding protein 3 (Hint3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276433.1

ORF Insert Sequence:

ATGGCTGAAA AGCAAGCGGG CTTAGCTGGA GAGCCGAATC CCGACTGTAC GGTCACTGCC 
AAAGCAGGAC CTGAGGTGTC CTCGCCTGGG ACCTCGGAGT CCAGGGACTA TGACAGTAAC 
TGCGTGTTCT GCCGCGTCGC GGCGGGTCAG GAACCCGAAA CTGAACTCTT ATACTGTGAG 
AACAAAGACC TGGTTTGTTT CAAAGATATC AAACCAGCAG CACTTCACCA TTACCTCGTG 
GTGCCAAAAA AGCATATTGG AAGTTGCAAG GATCTAAACA AAGATCACAT AGAAATGGTT 
GAGAGCATGG TCACTGTTGG AAAAACCATT CTTGAGAGGA ATAATTTCAC AGACTTCACA 
GATGTGAGGT TGATTATTTG CTTGAAAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence that human histidine triad nucleotide binding protein 3 (Hint3) is a distinct branch of the histidine triad (HIT) superfamily.
Journal of molecular biology373(4)978-89(2007 Nov)
Chou TF,Cheng J,Tikh IB,Wagner CR

Hint, Fhit, and GalT: function, structure, evolution, and mechanism of three branches of the histidine triad superfamily of nucleotide hydrolases and transferases.
Biochemistry41(29)9003-14(2002 Jul)
Brenner C