Retsat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Retsat
Entrez Gene ID	246298
Full Name	retinol saturase
Synonyms	Rmt7
General protein information	Preferred Names retinol saturase Names all-trans-retinol 13,14-reductase PPAR-alpha-regulated and starvation-induced gene protein RMT-7 all-trans-13,14-dihydroretinol saturase hypothetical protein RMT-7 retinol saturase (all trans retinol 13,14 reductase)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	mRNA displays differential expression in methylnitrosourea-induced mammary carcinomas [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_145084.1	NP_659552.1	all-trans-retinol 13,14-reductase precursor

Pathways from BioSystems

Homologies

	RETSAT	XP_001083594.1
Canis lupus familiaris (dog)	RETSAT	XP_540198.1
Bos taurus (cattle)	RETSAT	NP_001095749.2
Gallus gallus (chicken)	RETSAT	XP_424187.3
Oryza sativa (rice)	Os03g0841900	NP_001051855.1
Rattus norvegicus (Norway rat)	Retsat	NP_659552.1
Mus musculus (house mouse)	Retsat	NP_080435.3
Arabidopsis thaliana (thale cress)	AT1G57770	NP_176088.2
Xenopus tropicalis (tropical clawed frog)	retsat	NP_001121412.1
Danio rerio (zebrafish)	retsat	NP_001015061.1
Homo sapiens (human)	RETSAT	NP_060220.3
Pan troglodytes (chimpanzee)	RETSAT	XP_525801.2
Danio rerio (zebrafish)	zgc:154169	NP_001071261.1

Gene Ontology
Bibliography

Related articles in PubMed

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Identification of all-trans-retinol:all-trans-13,14-dihydroretinol saturase.
Moise AR, Kuksa V, Imanishi Y, Palczewski K
The Journal of biological chemistry279(48)50230-42(2004 Nov)

Altered gene expression profile in chemically induced rat mammary adenocarcinomas and its modulation by an aromatase inhibitor.
Wang Y, Hu L, Yao R, Wang M, Crist KA, Grubbs CJ, Johanning GL, Lubet RA, You M
Oncogene20(53)7710-21(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Retsat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Retsat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02533	NM_145084.1 Latest version!	Rattus norvegicus retinol saturase (Retsat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Retsat ( NM_145084.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_659552.1 Rattus norvegicus retinol saturase (Retsat), mRNA.

Price & Availability↑

CloneID	ORa02533
Clone ID Related Accession (Same CDS sequence)	NM_145084.1
Accession Version	NM_145084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-08
Organism	Rattus norvegicus(Norway rat)
Product	all-trans-retinol 13,14-reductase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF465614.1. On Sep 24, 2004 this sequence version replaced XM_346698.1. ##Evidence-Data-START## Transcript exon combination :: AF465614.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTGGATCA CTGCTCTGCT GCTGGTCGTG CTGCTGCTGG TGGTCGTTCA CAGGGTTTAT 
GTGGGCCTTT TCACCGGAAG TTCCCCGAAC CCCTTCGCTG AGGATGTCAA GCGGCCACCT 
GAACCCCTAG TGACCGACAA GGAGGCTAGG AAGAAAGTTC TCAAACAAGC TTTCTCAGTC 
AGCCGAGTAC CAGAGAAGCT GGACGCAGTG GTGATTGGCA GCGGCATTGG GGGACTGGCC 
TCAGCTGCGG TTCTAGCTAA AGCTGGCAAG AGAGTCCTTG TGCTGGAACA ACACACCAAG 
GCAGGCGGCT GTTGTCATAC CTTTGGGGAA AATGGCCTTG AATTTGACAC TGGAATTCAT 
TATATTGGAC GAATGCGGGA GGGCAACATT GGCCGTTTCA TCTTGGACCA GATCACCGAA 
GGGCAACTGG ACTGGGCCCC TATGGCCTCC CCTTTTGACT TGATGATACT AGAAGGACCC 
AATGGCCGAA AGGAGTTCCC CATGTACAGT GGGAGGAAAG AATACATCCA GGGCCTTAAG 
GAGAAGTTCC CCAAGGAAGA GGCTGTCATT GACAAATACA TGGAGCTGGT TAAGGTGGTG 
GCCCATGGAG TCTCTCATGC CATCCTACTG AAGTTCCTCC CATTGCCCTT GACTCAGCTC 
CTCAACAAGT TTGGGCTGCT GACTCGTTTT TCTCCATTCT GCCGAGCGTC TACTCAGAGC 
CTAGCTGAAG TCCTGAAACA GCTTGGGGCT TCCCCTGAGC TCCAGGCTGT TCTTAGCTAC 
ATTTTACCCA CTTACGGAGT AACCCCCAGC CATACCACCT TTTCCTTGCA CGCTCTGCTG 
GTTGACCACT ACATACAAGG GGCATATTAC CCCCGACGGG GCTCCAGTGA GATTGCTTTC 
CATACCATCC CTTTGATTCA GCGGGCCGGG GGCGCTGTCC TCACCAGGGC CACCGTACAG 
AGTGTGCTGC TGGACTCAGC TGGGAGAGCG TGCGGTGTCA GTGTGAAGAA GGGACAAGAG 
CTGGTGAACA TCTACTGCCC AGTTGTCATC TCCAATGCGG GAATGTTCAA TACCTATCAG 
CACCTGTTGC CAGAGTCTGT CCGCTACCTG CCAGATGTGA AGAAGCAGCT GACAATGGTA 
AAGCCTGGTT TGAGCATGCT GTCAATTTTC ATCTGTCTGA AGGGCACCAA GGAGGAGCTC 
AAGCTTCAGT CCACCAACTA CTATGTTTAT TTTGACACAG ACATGGACAA AGCGATGGAG 
TGCTATGTCT CTATGCCCAA GGAAAAGGCT CCAGAACACA TTCCCCTTCT CTTCATTCCC 
TTCCCATCAA GCAAGGATCC AACCTGGGAG GACCGATTCC CAGACCGATC CACAATGACT 
GTCCTGGTAC CCACGGCCTT TGAATGGTTC GAGGAGTGGC AGGAGGAGCC TAAGGGCAAG 
CGAGGTGTTG ACTATGAGAC CCTCAAAAAT ACCTTCCGGG AAGCCTCTAT GTCGGTGATC 
ATGAAACTGT TCCCACAGCT GGAAGGCAAG GTGGAGAGTG TGACTGGAGG ATCCCCACTG 
ACCAACCAGT ACTATCTGGC TGCACACCGA GGAGCTACTT ATGGGGCTGA CCATGACCTG 
GCTCGTCTGC ATCCTCATGC AATGGCTTCC CTAAGAGCCC AAACCCCCAT CCCCAACCTC 
TACCTGACAG GCCAAGATAT CTTCACCTGT GGGCTGATGG GGGCCCTGCA AGGGGCCCTG 
CTGTGCAGCA GTGCCATCCT GAAACGGAAC CTGTACTCAG ATCTGCAGGC TCTTGGCTCA 
AAGGTCAGGG CACAAAAGAA GAAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659552.1
CDS	1..1830
Translation	MWITALLLVVLLLVVVHRVYVGLFTGSSPNPFAEDVKRPPEPLVTDKEARKKVLKQAFSVSRVPEKLDAVVIGSGIGGLASAAVLAKAGKRVLVLEQHTKAGGCCHTFGENGLEFDTGIHYIGRMREGNIGRFILDQITEGQLDWAPMASPFDLMILEGPNGRKEFPMYSGRKEYIQGLKEKFPKEEAVIDKYMELVKVVAHGVSHAILLKFLPLPLTQLLNKFGLLTRFSPFCRASTQSLAEVLKQLGASPELQAVLSYILPTYGVTPSHTTFSLHALLVDHYIQGAYYPRRGSSEIAFHTIPLIQRAGGAVLTRATVQSVLLDSAGRACGVSVKKGQELVNIYCPVVISNAGMFNTYQHLLPESVRYLPDVKKQLTMVKPGLSMLSIFICLKGTKEELKLQSTNYYVYFDTDMDKAMECYVSMPKEKAPEHIPLLFIPFPSSKDPTWEDRFPDRSTMTVLVPTAFEWFEEWQEEPKGKRGVDYETLKNTFREASMSVIMKLFPQLEGKVESVTGGSPLTNQYYLAAHRGATYGADHDLARLHPHAMASLRAQTPIPNLYLTGQDIFTCGLMGALQGALLCSSAILKRNLYSDLQALGSKVRAQKKKK

Target ORF information:

RefSeq Version	NM_145084.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus retinol saturase (Retsat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145084.1

ORF Insert Sequence:

ATGTGGATCA CTGCTCTGCT GCTGGTCGTG CTGCTGCTGG TGGTCGTTCA CAGGGTTTAT 
GTGGGCCTTT TCACCGGAAG TTCCCCGAAC CCCTTCGCTG AGGATGTCAA GCGGCCACCT 
GAACCCCTAG TGACCGACAA GGAGGCTAGG AAGAAAGTTC TCAAACAAGC TTTCTCAGTC 
AGCCGAGTAC CAGAGAAGCT GGACGCAGTG GTGATTGGCA GCGGCATTGG GGGACTGGCC 
TCAGCTGCGG TTCTAGCTAA AGCTGGCAAG AGAGTCCTTG TGCTGGAACA ACACACCAAG 
GCAGGCGGCT GTTGTCATAC CTTTGGGGAA AATGGCCTTG AATTTGACAC TGGAATTCAT 
TATATTGGAC GAATGCGGGA GGGCAACATT GGCCGTTTCA TCTTGGACCA GATCACCGAA 
GGGCAACTGG ACTGGGCCCC TATGGCCTCC CCTTTTGACT TGATGATACT AGAAGGACCC 
AATGGCCGAA AGGAGTTCCC CATGTACAGT GGGAGGAAAG AATACATCCA GGGCCTTAAG 
GAGAAGTTCC CCAAGGAAGA GGCTGTCATT GACAAATACA TGGAGCTGGT TAAGGTGGTG 
GCCCATGGAG TCTCTCATGC CATCCTACTG AAGTTCCTCC CATTGCCCTT GACTCAGCTC 
CTCAACAAGT TTGGGCTGCT GACTCGTTTT TCTCCATTCT GCCGAGCGTC TACTCAGAGC 
CTAGCTGAAG TCCTGAAACA GCTTGGGGCT TCCCCTGAGC TCCAGGCTGT TCTTAGCTAC 
ATTTTACCCA CTTACGGAGT AACCCCCAGC CATACCACCT TTTCCTTGCA CGCTCTGCTG 
GTTGACCACT ACATACAAGG GGCATATTAC CCCCGACGGG GCTCCAGTGA GATTGCTTTC 
CATACCATCC CTTTGATTCA GCGGGCCGGG GGCGCTGTCC TCACCAGGGC CACCGTACAG 
AGTGTGCTGC TGGACTCAGC TGGGAGAGCG TGCGGTGTCA GTGTGAAGAA GGGACAAGAG 
CTGGTGAACA TCTACTGCCC AGTTGTCATC TCCAATGCGG GAATGTTCAA TACCTATCAG 
CACCTGTTGC CAGAGTCTGT CCGCTACCTG CCAGATGTGA AGAAGCAGCT GACAATGGTA 
AAGCCTGGTT TGAGCATGCT GTCAATTTTC ATCTGTCTGA AGGGCACCAA GGAGGAGCTC 
AAGCTTCAGT CCACCAACTA CTATGTTTAT TTTGACACAG ACATGGACAA AGCGATGGAG 
TGCTATGTCT CTATGCCCAA GGAAAAGGCT CCAGAACACA TTCCCCTTCT CTTCATTCCC 
TTCCCATCAA GCAAGGATCC AACCTGGGAG GACCGATTCC CAGACCGATC CACAATGACT 
GTCCTGGTAC CCACGGCCTT TGAATGGTTC GAGGAGTGGC AGGAGGAGCC TAAGGGCAAG 
CGAGGTGTTG ACTATGAGAC CCTCAAAAAT ACCTTCCGGG AAGCCTCTAT GTCGGTGATC 
ATGAAACTGT TCCCACAGCT GGAAGGCAAG GTGGAGAGTG TGACTGGAGG ATCCCCACTG 
ACCAACCAGT ACTATCTGGC TGCACACCGA GGAGCTACTT ATGGGGCTGA CCATGACCTG 
GCTCGTCTGC ATCCTCATGC AATGGCTTCC CTAAGAGCCC AAACCCCCAT CCCCAACCTC 
TACCTGACAG GCCAAGATAT CTTCACCTGT GGGCTGATGG GGGCCCTGCA AGGGGCCCTG 
CTGTGCAGCA GTGCCATCCT GAAACGGAAC CTGTACTCAG ATCTGCAGGC TCTTGGCTCA 
AAGGTCAGGG CACAAAAGAA GAAGAAGTAG