Ddx46 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx46
Entrez Gene ID	245957
Full Name	DEAD-box helicase 46
General protein information	Preferred Names DEAD-box helicase 46 Names probable ATP-dependent RNA helicase DDX46 DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 DEAD box protein 46 RNA helicase helicase of 117.4 kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	intranuclear RNA helicase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_139098.1	NP_620798.1	probable ATP-dependent RNA helicase DDX46
XM_006253601.3	XP_006253663.1	probable ATP-dependent RNA helicase DDX46 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX46	NP_055644.2
	DDX46	XP_002804561.1
Mus musculus (house mouse)	Ddx46	NP_001268984.1
Neurospora crassa	NCU02696	XP_965469.1
Canis lupus familiaris (dog)	DDX46	XP_531911.2
Rattus norvegicus (Norway rat)	Ddx46	NP_620798.1
Drosophila melanogaster (fruit fly)	CG6227	NP_573020.2
Oryza sativa (rice)	Os08g0159900	NP_001061045.1
Xenopus tropicalis (tropical clawed frog)	ddx46	XP_004912842.1
Pan troglodytes (chimpanzee)	DDX46	XP_517939.3
Danio rerio (zebrafish)	ddx46	NP_001019988.1
Magnaporthe oryzae (rice blast fungus)	MGG_15532	XP_003713486.1
Arabidopsis thaliana (thale cress)	AT3G09620	NP_187573.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010656	XP_311375.4
Caenorhabditis elegans (roundworm)	CELE_F53H1.1	NP_001033411.1
Arabidopsis thaliana (thale cress)	AT1G20920	NP_173516.1
Gallus gallus (chicken)	DDX46	XP_004945052.1
Schizosaccharomyces pombe (fission yeast)	prp11	NP_587856.1

Gene Ontology
Bibliography

Related articles in PubMed

Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing.
Chang TH, Tung L, Yeh FL, Chen JH, Chang SL
Biochimica et biophysica acta1829(8)764-74(2013 Aug)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins.
Sigal A, Milo R, Cohen A, Geva-Zatorsky N, Klein Y, Alaluf I, Swerdlin N, Perzov N, Danon T, Liron Y, Raveh T, Carpenter AE, Lahav G, Alon U
Nature methods3(7)525-31(2006 Jul)

A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor.
Sukegawa J, Blobel G
The Journal of biological chemistry270(26)15702-6(1995 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx46 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx46 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02158	NM_139098.1 Latest version!	Rattus norvegicus DEAD-box helicase 46 (Ddx46), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa37674	XM_006253601.3 Latest version!	Rattus norvegicus DEAD-box helicase 46 (Ddx46), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx46 ( NM_139098.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620798.1 Rattus norvegicus DEAD-box helicase 46 (Ddx46), mRNA.

Price & Availability↑

CloneID	ORa02158
Clone ID Related Accession (Same CDS sequence)	NM_139098.1
Accession Version	NM_139098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX46
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U25746.1. ##Evidence-Data-START## Transcript exon combination :: U25746.1, BC107590.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAGGG AGTCTCGCCA TTACCGAAAA CGGTCAGCAT CTCGAGGTCG CTCTGGAAGT 
CGGTCTAGGA GTCGCTCACC CTCTGACAAA AGAAGCAAAC GTGGAGATGA CAGGCGTTCC 
AGGAGTAGAG ACAGAGACAG AAGGAGAGAG AGGTCTCGGA GCAGGGACAA AAGGAGATCT 
CGGTCACGGG ACAGGAAACG CCTGAGACGT TCCAGAAGTA GAGAGCGTGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG CCGGGATAGA AGGCGCTCAA GGAGTCGGAG CCGGGGCAGG 
CGGTCCCGGT CTTCCAGTCC TGGCAGCAAA AGCAAGAAGG CTGAGAACAG GTCTAGGTCC 
AAAGAGAAAG CAGAAGGTGG GGACAGCTCC AAGGAGAAGA AGAAAGACAA GGACGACAAG 
GAGGACGAGA AAGAGAAGGA TGCTGGGAAT TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAACGAA AAGAAAGGGT TGAAAAATGG CGAGAAGAGC AGCGTAAAAA GGCGATGGAG 
AACATAGGGG AGCTGAAGAA GGAGATCGAG GAGATGAAGC AGGGGAAGAA GTGGAGCCTG 
GAAGATGACG ACGATGATGA AGATGACCCT GCCGAGGCTG AGAAGGAAGG GAATGAAATG 
GAGGACGAGG AGCTGGACCC ATTAGATGCC TACATGGAGG AAGTGAAAGA AGAGGTAAAA 
AAGTTTAACA TGAGGAGCGT GAAAGGCGGC GCAGGAAATG AGAAGAAGTC CGGACCGACT 
GTCACGAAAG TGGTTACTGT GGTAACAACC AAGAAAGCAG TAGTAGATGC CGACAAGAAG 
AAGGGAGAGC TGATGGAGAA TGACCAGGAC GCCATGGAGT ACTCCTCGGA GGAGGAAGAG 
GTTGACCTTC AGACGGCCCT CACAGGCTAC CAGACAAAAC AGAGAAAGCT TCTAGAGCCA 
GTGGATCATG GGAAAATTGA GTATGAGCCA TTCAGAAAAA ACTTTTACGT TGAAGTTCCA 
GAATTAGCGA AAATGTCTCA AGAAGAGGTG AATGTGTTTC GGTTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAGCCA ATTAAATCAT GGGTCCAGTG TGGAATTTCC 
ATGAAGATAC TGAATTCCCT CAAAAAGCAT GGCTATGAAA AGCCCACACC AATCCAAACT 
CAAGCAATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA GACAGGAAGT 
GGAAAAACCA TTGCTTTTCT TTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTG 
GAAGAAGGAG AGGGACCAAT CGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAA 
ATCACTAAAG AATGTAAGAA GTTTTCGAAG ACCTTGGGAC TGAGAGTGGT CTGTGTGTAT 
GGAGGGACAG GAATCAGCGA GCAGATTGCT GAGCTGAAGA GAGGCGCTGA GATCATTGTT 
TGCACACCTG GCCGGATGAT CGACATGCTG GCAGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCACAGGTCA TGCGCATTGT GGACAATGTC CGTCCTGACC GACAGACAGT TATGTTCTCA 
GCTACTTTCC CAAGAGCTAT GGAAGCGTTG GCCCGCAGGA TCTTGAGCAA GCCCATTGAA 
GTTCAGGTTG GAGGCCGGAG TGTGGTTTGC TCAGATGTGG AACAACAAGT GATTGTGATT 
GAAGAAGAGA AGAAATTCTT GAAATTGCTG GAGCTTTTAG GCCATTATCA GGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CACGCTGATG GTCTTCTGAA GGACTTGATG 
AGAGCTTCCT ACCCTTGCAT GTCTCTTCAC GGAGGTATTG ATCAGTATGA CAGGGACAGT 
ATCATAAATG ACTTTAAGAA TGGAACCTGC AAACTTCTCG TAGCCACGTC TGTTGCCGCC 
CGTGGTCTAG ATGTGAAACA TCTCATTCTT GTAGTAAATT ACAGCTGCCC CAATCATTAC 
GAAGATTACG TGCACAGGGC TGGGCGGACT GGAAGAGCAG GCAACAAGGG TTATGCCTAC 
ACCTTCATCA CAGAAGACCA AGCTCGATAT GCTGGGGACA TCATTAAAGC TCTTGAACTG 
TCGGGGACGG CGGTGCCTCC TGACCTAGAG AAACTATGGA GTGACTTCAA AGATCAGCAG 
AAAGCTGAAG GAAAAATAAT TAAAAAGAGC AGCGGCTTCT CTGGCAAGGG GTTCAAGTTT 
GATGAGACAG AGCAGGCTTT AGCCAATGAG AGGAAAAAGC TACAAAAAGC TGCCCTTGGC 
CTGCAGGATT CTGATGACGA GGACGCCGCA GTTGATATTG ACGAGCAGAT TGAAAGCATG 
TTTAATTCCA AGAAGAGAGT GAAAGACATG GCTGCTCCCG GCACATCCAG TGCGCCTGCT 
CCGACTGCGG GCAATGCTGA GAAGCTAGAG ATTGCCAAGA GGTTGGCGCT TAGAATCAAC 
GCTCAGAAGA ACCTGGGCAT CGAGTCTCAG GTAGATGTGA TGCAGCAGGC CACAAATGCC 
ATCCTCAGAG GTGGCCCTCT TCTGGCTCCC ACCGTGTCTG CAAAAACCAT CGCAGAGCAG 
CTTGCCGAGA AGATCAATGC CAAGCTCAAT TACGTGCCTT TGGAGAAACA GGAAGAAGAG 
AGACAGGAGG GTGGGCAGAG CGAATCTTTC AAGAGATACG AGGAAGAATT AGAAATTAAT 
GACTTCCCAC AGACGGCGAG GTGGAAAGTT ACCTCTAAGG AAGCCTTGCA GAGGATCAGT 
GAGTACTCGG AAGCCGCCAT CACAATCAGA GGGACCTACT TCCCGCCTGG CAAAGAGCCC 
AAGGAAGGAG AGCGGAAGAT CTACTTGGCG ATTGAAAGTG CCAACGAGCT GGCTGTGCAG 
AAAGCAAAGG CAGAGATCAC CAGGCTCATA AAAGAGGAGC TGATCCGACT GCAAAATTCA 
TACCAACCCA CCAACAAAGG ACGATACAAA GTATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620798.1
CDS	153..3251
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGSKSKKAENRSRSKEKAEGGDSSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEDEELDPLDAYMEEVKEEVKKFNMRSVKGGAGNEKKSGPTVTKVVTVVTTKKAVVDADKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSAPAPTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGPLLAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQEGGQSESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL

Target ORF information:

RefSeq Version	NM_139098.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 46 (Ddx46), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139098.1

ORF Insert Sequence:

ATGGGCAGGG AGTCTCGCCA TTACCGAAAA CGGTCAGCAT CTCGAGGTCG CTCTGGAAGT 
CGGTCTAGGA GTCGCTCACC CTCTGACAAA AGAAGCAAAC GTGGAGATGA CAGGCGTTCC 
AGGAGTAGAG ACAGAGACAG AAGGAGAGAG AGGTCTCGGA GCAGGGACAA AAGGAGATCT 
CGGTCACGGG ACAGGAAACG CCTGAGACGT TCCAGAAGTA GAGAGCGTGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG CCGGGATAGA AGGCGCTCAA GGAGTCGGAG CCGGGGCAGG 
CGGTCCCGGT CTTCCAGTCC TGGCAGCAAA AGCAAGAAGG CTGAGAACAG GTCTAGGTCC 
AAAGAGAAAG CAGAAGGTGG GGACAGCTCC AAGGAGAAGA AGAAAGACAA GGACGACAAG 
GAGGACGAGA AAGAGAAGGA TGCTGGGAAT TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAACGAA AAGAAAGGGT TGAAAAATGG CGAGAAGAGC AGCGTAAAAA GGCGATGGAG 
AACATAGGGG AGCTGAAGAA GGAGATCGAG GAGATGAAGC AGGGGAAGAA GTGGAGCCTG 
GAAGATGACG ACGATGATGA AGATGACCCT GCCGAGGCTG AGAAGGAAGG GAATGAAATG 
GAGGACGAGG AGCTGGACCC ATTAGATGCC TACATGGAGG AAGTGAAAGA AGAGGTAAAA 
AAGTTTAACA TGAGGAGCGT GAAAGGCGGC GCAGGAAATG AGAAGAAGTC CGGACCGACT 
GTCACGAAAG TGGTTACTGT GGTAACAACC AAGAAAGCAG TAGTAGATGC CGACAAGAAG 
AAGGGAGAGC TGATGGAGAA TGACCAGGAC GCCATGGAGT ACTCCTCGGA GGAGGAAGAG 
GTTGACCTTC AGACGGCCCT CACAGGCTAC CAGACAAAAC AGAGAAAGCT TCTAGAGCCA 
GTGGATCATG GGAAAATTGA GTATGAGCCA TTCAGAAAAA ACTTTTACGT TGAAGTTCCA 
GAATTAGCGA AAATGTCTCA AGAAGAGGTG AATGTGTTTC GGTTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAGCCA ATTAAATCAT GGGTCCAGTG TGGAATTTCC 
ATGAAGATAC TGAATTCCCT CAAAAAGCAT GGCTATGAAA AGCCCACACC AATCCAAACT 
CAAGCAATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA GACAGGAAGT 
GGAAAAACCA TTGCTTTTCT TTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTG 
GAAGAAGGAG AGGGACCAAT CGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAA 
ATCACTAAAG AATGTAAGAA GTTTTCGAAG ACCTTGGGAC TGAGAGTGGT CTGTGTGTAT 
GGAGGGACAG GAATCAGCGA GCAGATTGCT GAGCTGAAGA GAGGCGCTGA GATCATTGTT 
TGCACACCTG GCCGGATGAT CGACATGCTG GCAGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCACAGGTCA TGCGCATTGT GGACAATGTC CGTCCTGACC GACAGACAGT TATGTTCTCA 
GCTACTTTCC CAAGAGCTAT GGAAGCGTTG GCCCGCAGGA TCTTGAGCAA GCCCATTGAA 
GTTCAGGTTG GAGGCCGGAG TGTGGTTTGC TCAGATGTGG AACAACAAGT GATTGTGATT 
GAAGAAGAGA AGAAATTCTT GAAATTGCTG GAGCTTTTAG GCCATTATCA GGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CACGCTGATG GTCTTCTGAA GGACTTGATG 
AGAGCTTCCT ACCCTTGCAT GTCTCTTCAC GGAGGTATTG ATCAGTATGA CAGGGACAGT 
ATCATAAATG ACTTTAAGAA TGGAACCTGC AAACTTCTCG TAGCCACGTC TGTTGCCGCC 
CGTGGTCTAG ATGTGAAACA TCTCATTCTT GTAGTAAATT ACAGCTGCCC CAATCATTAC 
GAAGATTACG TGCACAGGGC TGGGCGGACT GGAAGAGCAG GCAACAAGGG TTATGCCTAC 
ACCTTCATCA CAGAAGACCA AGCTCGATAT GCTGGGGACA TCATTAAAGC TCTTGAACTG 
TCGGGGACGG CGGTGCCTCC TGACCTAGAG AAACTATGGA GTGACTTCAA AGATCAGCAG 
AAAGCTGAAG GAAAAATAAT TAAAAAGAGC AGCGGCTTCT CTGGCAAGGG GTTCAAGTTT 
GATGAGACAG AGCAGGCTTT AGCCAATGAG AGGAAAAAGC TACAAAAAGC TGCCCTTGGC 
CTGCAGGATT CTGATGACGA GGACGCCGCA GTTGATATTG ACGAGCAGAT TGAAAGCATG 
TTTAATTCCA AGAAGAGAGT GAAAGACATG GCTGCTCCCG GCACATCCAG TGCGCCTGCT 
CCGACTGCGG GCAATGCTGA GAAGCTAGAG ATTGCCAAGA GGTTGGCGCT TAGAATCAAC 
GCTCAGAAGA ACCTGGGCAT CGAGTCTCAG GTAGATGTGA TGCAGCAGGC CACAAATGCC 
ATCCTCAGAG GTGGCCCTCT TCTGGCTCCC ACCGTGTCTG CAAAAACCAT CGCAGAGCAG 
CTTGCCGAGA AGATCAATGC CAAGCTCAAT TACGTGCCTT TGGAGAAACA GGAAGAAGAG 
AGACAGGAGG GTGGGCAGAG CGAATCTTTC AAGAGATACG AGGAAGAATT AGAAATTAAT 
GACTTCCCAC AGACGGCGAG GTGGAAAGTT ACCTCTAAGG AAGCCTTGCA GAGGATCAGT 
GAGTACTCGG AAGCCGCCAT CACAATCAGA GGGACCTACT TCCCGCCTGG CAAAGAGCCC 
AAGGAAGGAG AGCGGAAGAT CTACTTGGCG ATTGAAAGTG CCAACGAGCT GGCTGTGCAG 
AAAGCAAAGG CAGAGATCAC CAGGCTCATA AAAGAGGAGC TGATCCGACT GCAAAATTCA 
TACCAACCCA CCAACAAAGG ACGATACAAA GTATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx46 ( XM_006253601.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253663.1 Rattus norvegicus DEAD-box helicase 46 (Ddx46), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa37674
Clone ID Related Accession (Same CDS sequence)	XM_006253601.3
Accession Version	XM_006253601.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX46 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253601.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGGG AGTCTCGCCA TTACCGAAAA CGGTCAGCAT CTCGAGGTCG CTCTGGAAGT 
CGGTCTAGGA GTCGCTCACC CTCTGACAAA AGAAGCAAAC GTGGAGATGA CAGGCGTTCC 
AGGAGTAGAG ACAGAGACAG AAGGAGAGAG AGGTCTCGGA GCAGGGACAA AAGGAGATCT 
CGGTCACGGG ACAGGAAACG CCTGAGACGT TCCAGAAGTA GAGAGCGTGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG CCGGGATAGA AGGCGCTCAA GGAGTCGGAG CCGGGGCAGG 
CGGTCCCGGT CTTCCAGTCC TGGCAGCAAA AGCAAGAAAG CTGAGAACAG GTCTAGGTCC 
AAAGAGAAAG CAGAAGGTGG GGACAGCTCC AAGGAGAAGA AGAAAGACAA GGACGACAAG 
GAGGACGAGA AAGAGAAGGA TGCTGGGAAT TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAACGAA AAGAAAGGGT TGAAAAATGG CGAGAAGAGC AGCGTAAAAA GGCGATGGAG 
AACATAGGGG AGCTGAAGAA GGAGATCGAG GAGATGAAGC AGGGGAAGAA GTGGAGCCTG 
GAAGATGACG ACGATGATGA AGATGACCCT GCCGAGGCTG AGAAGGAAGG GAATGAAATG 
GAGGACGAGG AGCTGGACCC ATTAGATGCC TACATGGAGG AAGTGAAAGA AGAGGTAAAA 
AAGTTTAACA TGAGGAGCGT GAAAGGCGGC GCAGGAAATG AGAAGAAGTC CGGACCGACT 
GTCACGAAAG TGGTTACTGT GGTAACAACC AAGAAAGCAG TAGTAGATGC CGACAAGAAG 
AAGGGAGAGC TGATGGAGAA TGACCAGGAC GCCATGGAGT ACTCCTCGGA GGAGGAAGAG 
GTTGACCTTC AGACGGCCCT CACAGGCTAC CAGACAAAAC AGAGAAAGCT TCTAGAGCCA 
GTGGATCATG GGAAAATTGA GTATGAGCCA TTCAGAAAAA ACTTTTACGT TGAAGTTCCA 
GAATTAGCGA AAATGTCTCA AGAAGAGGTG AATGTGTTTC GGTTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAGCCA ATTAAATCAT GGGTCCAGTG TGGAATTTCC 
ATGAAGATAC TGAATTCCCT CAAAAAGCAT GGCTATGAAA AGCCCACACC AATCCAAACT 
CAAGCAATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA GACAGGAAGT 
GGAAAAACCA TTGCTTTTCT TTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTG 
GAAGAAGGAG AGGGACCAAT CGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAA 
ATCACTAAAG AATGTAAGAA GTTTTCGAAG ACCTTGGGAC TGAGAGTGGT CTGTGTGTAT 
GGAGGGACAG GAATCAGCGA GCAGATTGCT GAGCTGAAGA GAGGCGCTGA GATCATTGTT 
TGCACACCTG GCCGGATGAT CGACATGCTG GCAGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCACAGGTCA TGCGCATTGT GGACAATGTC CGTCCTGACC GACAGACAGT TATGTTCTCA 
GCTACTTTCC CAAGAGCTAT GGAAGCGTTG GCCCGCAGGA TCTTGAGCAA GCCCATTGAA 
GTTCAGGTTG GAGGCCGGAG TGTGGTTTGC TCAGATGTGG AACAACAAGT GATTGTGATT 
GAAGAAGAGA AGAAATTCTT GAAATTGCTG GAGCTTTTAG GCCATTATCA GGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CACGCTGATG GTCTTCTGAA GGACTTGATG 
AGAGCTTCCT ACCCTTGCAT GTCTCTTCAC GGAGGTATTG ATCAGTATGA CAGGGACAGT 
ATCATAAATG ACTTTAAGAA TGGAACCTGC AAACTTCTCG TAGCCACGTC TGTTGCCGCC 
CGTGGTCTAG ATGTGAAACA TCTCATTCTT GTAGTAAATT ACAGCTGCCC CAATCATTAC 
GAAGATTACG TGCACAGGGC TGGGCGGACT GGAAGAGCAG GCAACAAGGG TTATGCCTAC 
ACCTTCATCA CAGAAGACCA AGCTCGATAT GCTGGGGACA TCATTAAAGC TCTTGAACTG 
TCGGGGACGG CGGTGCCTCC TGACCTAGAG AAACTATGGA GTGACTTCAA AGATCAGCAG 
AAAGCTGAAG GAAAAATAAT TAAAAAGAGC AGCGGCTTCT CTGGCAAGGG GTTCAAGTTT 
GATGAGACAG AGCAGGCTTT AGCCAATGAG AGGAAAAAGC TACAAAAAGC TGCCCTTGGC 
CTGCAGGATT CTGATGACGA GGACGCCGCA GTTGATATTG ACGAGCAGAT TGAAAGCATG 
TTTAATTCCA AGAAGAGAGT GAAAGACATG GCTGCTCCCG GCACATCCAG TGCGCCTGCT 
CCGACTGCGG GCAATGCTGA GAAGCTAGAG ATTGCCAAGA GGTTGGCGCT TAGAATCAAC 
GCTCAGAAGA ACCTGGGCAT CGAGTCTCAG GATGTGATGC AGCAGGCCAC AAATGCCATC 
CTCAGAGGTG GCCCTCTTCT GGCTCCCACC GTGTCTGCAA AAACCATCGC AGAGCAGCTT 
GCCGAGAAGA TCAATGCCAA GCTCAATTAC GTGCCTTTGG AGAAACAGGA AGAAGAGAGA 
CAGGAGGGTG GGCAGAGCGA ATCTTTCAAG AGATACGAGG AAGAATTAGA AATTAATGAC 
TTCCCACAGA CGGCGAGGTG GAAAGTTACC TCTAAGGAAG CCTTGCAGAG GATCAGTGAG 
TACTCGGAAG CCGCCATCAC AATCAGAGGG ACCTACTTCC CGCCTGGCAA AGAGCCCAAG 
GAAGGAGAGC GGAAGATCTA CTTGGCGATT GAAAGTGCCA ACGAGCTGGC TGTGCAGAAA 
GCAAAGGCAG AGATCACCAG GCTCATAAAA GAGGAGCTGA TCCGACTGCA AAATTCATAC 
CAACCCACCA ACAAAGGACG ATACAAAGTA TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253663.1
CDS	184..3279
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGSKSKKAENRSRSKEKAEGGDSSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEDEELDPLDAYMEEVKEEVKKFNMRSVKGGAGNEKKSGPTVTKVVTVVTTKKAVVDADKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSAPAPTAGNAEKLEIAKRLALRINAQKNLGIESQDVMQQATNAILRGGPLLAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQEGGQSESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL

Target ORF information:

RefSeq Version	XM_006253601.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 46 (Ddx46), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253601.3

ORF Insert Sequence:

ATGGGCAGGG AGTCTCGCCA TTACCGAAAA CGGTCAGCAT CTCGAGGTCG CTCTGGAAGT 
CGGTCTAGGA GTCGCTCACC CTCTGACAAA AGAAGCAAAC GTGGAGATGA CAGGCGTTCC 
AGGAGTAGAG ACAGAGACAG AAGGAGAGAG AGGTCTCGGA GCAGGGACAA AAGGAGATCT 
CGGTCACGGG ACAGGAAACG CCTGAGACGT TCCAGAAGTA GAGAGCGTGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG CCGGGATAGA AGGCGCTCAA GGAGTCGGAG CCGGGGCAGG 
CGGTCCCGGT CTTCCAGTCC TGGCAGCAAA AGCAAGAAAG CTGAGAACAG GTCTAGGTCC 
AAAGAGAAAG CAGAAGGTGG GGACAGCTCC AAGGAGAAGA AGAAAGACAA GGACGACAAG 
GAGGACGAGA AAGAGAAGGA TGCTGGGAAT TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAACGAA AAGAAAGGGT TGAAAAATGG CGAGAAGAGC AGCGTAAAAA GGCGATGGAG 
AACATAGGGG AGCTGAAGAA GGAGATCGAG GAGATGAAGC AGGGGAAGAA GTGGAGCCTG 
GAAGATGACG ACGATGATGA AGATGACCCT GCCGAGGCTG AGAAGGAAGG GAATGAAATG 
GAGGACGAGG AGCTGGACCC ATTAGATGCC TACATGGAGG AAGTGAAAGA AGAGGTAAAA 
AAGTTTAACA TGAGGAGCGT GAAAGGCGGC GCAGGAAATG AGAAGAAGTC CGGACCGACT 
GTCACGAAAG TGGTTACTGT GGTAACAACC AAGAAAGCAG TAGTAGATGC CGACAAGAAG 
AAGGGAGAGC TGATGGAGAA TGACCAGGAC GCCATGGAGT ACTCCTCGGA GGAGGAAGAG 
GTTGACCTTC AGACGGCCCT CACAGGCTAC CAGACAAAAC AGAGAAAGCT TCTAGAGCCA 
GTGGATCATG GGAAAATTGA GTATGAGCCA TTCAGAAAAA ACTTTTACGT TGAAGTTCCA 
GAATTAGCGA AAATGTCTCA AGAAGAGGTG AATGTGTTTC GGTTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAGCCA ATTAAATCAT GGGTCCAGTG TGGAATTTCC 
ATGAAGATAC TGAATTCCCT CAAAAAGCAT GGCTATGAAA AGCCCACACC AATCCAAACT 
CAAGCAATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA GACAGGAAGT 
GGAAAAACCA TTGCTTTTCT TTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTG 
GAAGAAGGAG AGGGACCAAT CGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAA 
ATCACTAAAG AATGTAAGAA GTTTTCGAAG ACCTTGGGAC TGAGAGTGGT CTGTGTGTAT 
GGAGGGACAG GAATCAGCGA GCAGATTGCT GAGCTGAAGA GAGGCGCTGA GATCATTGTT 
TGCACACCTG GCCGGATGAT CGACATGCTG GCAGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCACAGGTCA TGCGCATTGT GGACAATGTC CGTCCTGACC GACAGACAGT TATGTTCTCA 
GCTACTTTCC CAAGAGCTAT GGAAGCGTTG GCCCGCAGGA TCTTGAGCAA GCCCATTGAA 
GTTCAGGTTG GAGGCCGGAG TGTGGTTTGC TCAGATGTGG AACAACAAGT GATTGTGATT 
GAAGAAGAGA AGAAATTCTT GAAATTGCTG GAGCTTTTAG GCCATTATCA GGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CACGCTGATG GTCTTCTGAA GGACTTGATG 
AGAGCTTCCT ACCCTTGCAT GTCTCTTCAC GGAGGTATTG ATCAGTATGA CAGGGACAGT 
ATCATAAATG ACTTTAAGAA TGGAACCTGC AAACTTCTCG TAGCCACGTC TGTTGCCGCC 
CGTGGTCTAG ATGTGAAACA TCTCATTCTT GTAGTAAATT ACAGCTGCCC CAATCATTAC 
GAAGATTACG TGCACAGGGC TGGGCGGACT GGAAGAGCAG GCAACAAGGG TTATGCCTAC 
ACCTTCATCA CAGAAGACCA AGCTCGATAT GCTGGGGACA TCATTAAAGC TCTTGAACTG 
TCGGGGACGG CGGTGCCTCC TGACCTAGAG AAACTATGGA GTGACTTCAA AGATCAGCAG 
AAAGCTGAAG GAAAAATAAT TAAAAAGAGC AGCGGCTTCT CTGGCAAGGG GTTCAAGTTT 
GATGAGACAG AGCAGGCTTT AGCCAATGAG AGGAAAAAGC TACAAAAAGC TGCCCTTGGC 
CTGCAGGATT CTGATGACGA GGACGCCGCA GTTGATATTG ACGAGCAGAT TGAAAGCATG 
TTTAATTCCA AGAAGAGAGT GAAAGACATG GCTGCTCCCG GCACATCCAG TGCGCCTGCT 
CCGACTGCGG GCAATGCTGA GAAGCTAGAG ATTGCCAAGA GGTTGGCGCT TAGAATCAAC 
GCTCAGAAGA ACCTGGGCAT CGAGTCTCAG GATGTGATGC AGCAGGCCAC AAATGCCATC 
CTCAGAGGTG GCCCTCTTCT GGCTCCCACC GTGTCTGCAA AAACCATCGC AGAGCAGCTT 
GCCGAGAAGA TCAATGCCAA GCTCAATTAC GTGCCTTTGG AGAAACAGGA AGAAGAGAGA 
CAGGAGGGTG GGCAGAGCGA ATCTTTCAAG AGATACGAGG AAGAATTAGA AATTAATGAC 
TTCCCACAGA CGGCGAGGTG GAAAGTTACC TCTAAGGAAG CCTTGCAGAG GATCAGTGAG 
TACTCGGAAG CCGCCATCAC AATCAGAGGG ACCTACTTCC CGCCTGGCAA AGAGCCCAAG 
GAAGGAGAGC GGAAGATCTA CTTGGCGATT GAAAGTGCCA ACGAGCTGGC TGTGCAGAAA 
GCAAAGGCAG AGATCACCAG GCTCATAAAA GAGGAGCTGA TCCGACTGCA AAATTCATAC 
CAACCCACCA ACAAAGGACG ATACAAAGTA TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functions of the DExD/H-box proteins in nuclear pre-mRNA splicing.
Biochimica et biophysica acta1829(8)764-74(2013 Aug)
Chang TH,Tung L,Yeh FL,Chen JH,Chang SL

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins.
Nature methods3(7)525-31(2006 Jul)
Sigal A,Milo R,Cohen A,Geva-Zatorsky N,Klein Y,Alaluf I,Swerdlin N,Perzov N,Danon T,Liron Y,Raveh T,Carpenter AE,Lahav G,Alon U

A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor.
The Journal of biological chemistry270(26)15702-6(1995 Jun)
Sukegawa J,Blobel G