Gad2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gad2
Entrez Gene ID	24380
Full Name	glutamate decarboxylase 2
Synonyms	gad65
General protein information	Preferred Names glutamate decarboxylase 2 Names glutamate decarboxylase 2 65 kDa glutamic acid decarboxylase GAD-65 Glutamate decarboxylase 2 (islet) glutamate decarboxylase 65 kDa isoform glutamic acid decarboxylase 2 glutamic acid decarboxylase 65
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	catalyzes synthesis of gamma-Aminobutyric acid (GABA), the most widely distributed inhibitory neurotransmitter [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012563.1	NP_036695.1	glutamate decarboxylase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GAD2	NP_000809.1
Pan troglodytes (chimpanzee)	GAD2	XP_001157825.1
	GAD2	XP_002805636.1
Bos taurus (cattle)	GAD2	NP_001192652.1
Gallus gallus (chicken)	GAD2	XP_418596.3
Xenopus tropicalis (tropical clawed frog)	gad2	XP_002933179.1
Canis lupus familiaris (dog)	GAD2	NP_001070907.1
Mus musculus (house mouse)	Gad2	NP_032104.2
Rattus norvegicus (Norway rat)	Gad2	NP_036695.1
Danio rerio (zebrafish)	gad2	NP_001017708.2

Gene Ontology
Bibliography

Related articles in PubMed

Acute high-intensity noise induces rapid Arc protein expression but fails to rapidly change GAD expression in amygdala and hippocampus of rats: Effects of treatment with D-cycloserine.
Kapolowicz MR, Thompson LT
Hearing research34269-79(2016 Dec)

Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity.
Phelps EA, Cianciaruso C, Michael IP, Pasquier M, Kanaani J, Nano R, Lavallard V, Billestrup N, Hubbell JA, Baekkeskov S
Diabetes65(9)2686-99(2016 09)

Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.
Wu W, Rahman MN, Guo J, Roy N, Xue L, Cahill CM, Zhang S, Jia Z
Journal of molecular cell biology7(2)168-79(2015 Apr)

Spatial and temporal pattern of changes in the number of GAD65-immunoreactive inhibitory terminals in the rat superficial dorsal horn following peripheral nerve injury.
Lorenzo LE, Magnussen C, Bailey AL, St Louis M, De Koninck Y, Ribeiro-da-Silva A
Molecular pain1057(2014 Sep)

Number and regional distribution of GAD65 mRNA-expressing interneurons in the rat hippocampal formation.
Cz?h B, Abrah?m H, Tahtakran S, Houser CR, Seress L
Acta biologica Hungarica64(4)395-413(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

No changes in either Arc or GAD protein expression were observed in primary auditory cortex in the immediate post-noise exposure period, confirming other reports that auditory cortical plasticity may not occur until later in the development of tinnitus.
Title: Acute high-intensity noise induces rapid Arc protein expression but fails to rapidly change GAD expression in amygdala and hippocampus of rats: Effects of treatment with D-cycloserine.

Study propose that aberrant accumulation of immunogenic GAD65 in Golgi membranes facilitates inappropriate presentation to the immune system after release from stressed and/or damaged beta-cells, triggering autoimmunity.
Title: Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity.

otoferlin, through GAD65, modulates GABAergic activity.
Title: Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.

This study demonistrated that GAD65+ inhibitory terminals revealed that nerve injury induced a transient loss of GAD65 immunoreactive terminals.
Title: Spatial and temporal pattern of changes in the number of GAD65-immunoreactive inhibitory terminals in the rat superficial dorsal horn following peripheral nerve injury.

GAD65 mRNA-expressing neurons are significantly more numerous in the dorsal than in the ventral hippocampus. Two-thirds of hippocampal interneurons are counted in Ammon's horn (61,590) and one-third in the dentate gyrus (28,000).
Title: Number and regional distribution of GAD65 mRNA-expressing interneurons in the rat hippocampal formation.

The following Gad2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gad2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00349	NM_012563.1 Latest version!	Rattus norvegicus glutamate decarboxylase 2 (Gad2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gad2 ( NM_012563.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036695.1 Rattus norvegicus glutamate decarboxylase 2 (Gad2), mRNA.

Price & Availability↑

CloneID	ORa00349
Clone ID Related Accession (Same CDS sequence)	NM_012563.1
Accession Version	NM_012563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate decarboxylase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M72422.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M72422.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCTC CGGGCTCTGG CTTTTGGTCC TTCGGATCTG AAGATGGCTC TGGGGATCCT 
GAGAACCCGG GAACAGCGAG AGCCTGGTGC CAGGTGGCCC AAAAGTTCAC GGGCGGCATC 
GGAAACAAGC TATGCGCTCT GCTCTACGGA GACTCTGAGA AGCCAGCAGA GAGCGGCGGG 
AGCGTGACCT CGCGGGCCGC CACTCGGAAG GTCGCCTGCA CCTGTGACCA AAAACCCTGC 
AGCTGCCCCA AAGGAGATGT CAATTATGCA CTTCTCCACG CAACAGACCT GCTGCCAGCC 
TGTGAAGGAG AAAGGCCCAC TCTCGCATTT CTGCAAGATG TAATGAACAT TTTGCTTCAG 
TACGTGGTGA AAAGTTTTGA TAGATCAACT AAAGTGATTG ATTTCCATTA CCCCAATGAG 
CTTCTTCAAG AGTATAATTG GGAATTGGCA GACCAACCGC AAAATCTGGA GGAAATTTTG 
ACGCACTGCC AAACAACTCT AAAATATGCG ATTAAAACAG GGCATCCCCG ATATTTTAAT 
CAGCTGTCTA CCGGATTGGA TATGGTTGGA TTAGCAGCAG ATTGGTTGAC ATCAACAGCA 
AACACGAACA TGTTTACCTA TGAGATCGCC CCTGTATTTG TACTACTGGA ATATGTGACA 
CTAAAGAAAA TGAGGGAAAT CATTGGCTGG CCAGGAGGCT CTGGCGATGG AATCTTTTCT 
CCTGGTGGTG CCATCTCCAA CATGTACGCC ATGCTCATTG CCCGCTATAA GATGTTTCCA 
GAAGTCAAGG AAAAGGGGAT GGCGGCGGTG CCCAGGCTCA TCGCATTCAC GTCAGAGCAT 
AGTCACTTTT CTCTCAAGAA GGGAGCTGCA GCCTTGGGGA TCGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATCCCATCTG ACCTTGAAAG AAGAATCCTT 
GAAGTCAAAC AGAAAGGATT TGTTCCTTTC CTGGTGAGTG CCACAGCTGG AACCACTGTG 
TACGGGGCTT TTGATCCTCT CTTGGCTGTA GCTGACATCT GCAAAAAATA TAAGATCTGG 
ATGCATGTGG ATGCTGCTTG GGGTGGAGGG TTACTGATGT CTCGGAAACA CAAGTGGAAG 
CTGAACGGTG TGGAGAGGGC CAACTCTGTG ACATGGAATC CCCACAAGAT GATGGGTGTC 
CCCTTGCAAT GTTCGGCTCT CCTGGTCAGA GAGGAGGGAC TGATGCAGAG CTGCAACCAG 
ATGCATGCTT CCTACCTCTT TCAGCAAGAT AAGCACTATG ACCTGTCCTA TGACACGGGA 
GACAAGGCCT TGCAGTGTGG ACGCCACGTC GATGTCTTTA AATTATGGCT CATGTGGAGA 
GCAAAGGGGA CTACTGGATT TGAAGCTCAC ATTGATAAGT GTTTGGAGCT GGCAGAGTAT 
TTATACAATA TCATTAAAAA CCGAGAAGGA TATGAAATGG TGTTCGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTTTGTACCT CCTAGTTTGC GAGTTCTGGA AGACAATGAA 
GAGAGAATGA GCCGCCTCTC AAAGGTGGCG CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGGACCACAA TGGTCAGCTA CCAACCCTTA GGAGATAAGG TCAACTTCTT CCGCATGGTC 
ATCTCAAACC CTGCAGCAAC TCACCAAGAC ATTGACTTCC TCATTGAAGA AATCGAACGC 
CTGGGACAAG ATTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036695.1
CDS	75..1832
Translation	MASPGSGFWSFGSEDGSGDPENPGTARAWCQVAQKFTGGIGNKLCALLYGDSEKPAESGGSVTSRAATRKVACTCDQKPCSCPKGDVNYALLHATDLLPACEGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILTHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLNGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWFVPPSLRVLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	NM_012563.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate decarboxylase 2 (Gad2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012563.1

ORF Insert Sequence:

ATGGCATCTC CGGGCTCTGG CTTTTGGTCC TTCGGATCTG AAGATGGCTC TGGGGATCCT 
GAGAACCCGG GAACAGCGAG AGCCTGGTGC CAGGTGGCCC AAAAGTTCAC GGGCGGCATC 
GGAAACAAGC TATGCGCTCT GCTCTACGGA GACTCTGAGA AGCCAGCAGA GAGCGGCGGG 
AGCGTGACCT CGCGGGCCGC CACTCGGAAG GTCGCCTGCA CCTGTGACCA AAAACCCTGC 
AGCTGCCCCA AAGGAGATGT CAATTATGCA CTTCTCCACG CAACAGACCT GCTGCCAGCC 
TGTGAAGGAG AAAGGCCCAC TCTCGCATTT CTGCAAGATG TAATGAACAT TTTGCTTCAG 
TACGTGGTGA AAAGTTTTGA TAGATCAACT AAAGTGATTG ATTTCCATTA CCCCAATGAG 
CTTCTTCAAG AGTATAATTG GGAATTGGCA GACCAACCGC AAAATCTGGA GGAAATTTTG 
ACGCACTGCC AAACAACTCT AAAATATGCG ATTAAAACAG GGCATCCCCG ATATTTTAAT 
CAGCTGTCTA CCGGATTGGA TATGGTTGGA TTAGCAGCAG ATTGGTTGAC ATCAACAGCA 
AACACGAACA TGTTTACCTA TGAGATCGCC CCTGTATTTG TACTACTGGA ATATGTGACA 
CTAAAGAAAA TGAGGGAAAT CATTGGCTGG CCAGGAGGCT CTGGCGATGG AATCTTTTCT 
CCTGGTGGTG CCATCTCCAA CATGTACGCC ATGCTCATTG CCCGCTATAA GATGTTTCCA 
GAAGTCAAGG AAAAGGGGAT GGCGGCGGTG CCCAGGCTCA TCGCATTCAC GTCAGAGCAT 
AGTCACTTTT CTCTCAAGAA GGGAGCTGCA GCCTTGGGGA TCGGAACAGA CAGCGTGATT 
CTGATTAAAT GTGATGAGAG AGGGAAAATG ATCCCATCTG ACCTTGAAAG AAGAATCCTT 
GAAGTCAAAC AGAAAGGATT TGTTCCTTTC CTGGTGAGTG CCACAGCTGG AACCACTGTG 
TACGGGGCTT TTGATCCTCT CTTGGCTGTA GCTGACATCT GCAAAAAATA TAAGATCTGG 
ATGCATGTGG ATGCTGCTTG GGGTGGAGGG TTACTGATGT CTCGGAAACA CAAGTGGAAG 
CTGAACGGTG TGGAGAGGGC CAACTCTGTG ACATGGAATC CCCACAAGAT GATGGGTGTC 
CCCTTGCAAT GTTCGGCTCT CCTGGTCAGA GAGGAGGGAC TGATGCAGAG CTGCAACCAG 
ATGCATGCTT CCTACCTCTT TCAGCAAGAT AAGCACTATG ACCTGTCCTA TGACACGGGA 
GACAAGGCCT TGCAGTGTGG ACGCCACGTC GATGTCTTTA AATTATGGCT CATGTGGAGA 
GCAAAGGGGA CTACTGGATT TGAAGCTCAC ATTGATAAGT GTTTGGAGCT GGCAGAGTAT 
TTATACAATA TCATTAAAAA CCGAGAAGGA TATGAAATGG TGTTCGATGG GAAGCCTCAG 
CACACAAATG TCTGCTTCTG GTTTGTACCT CCTAGTTTGC GAGTTCTGGA AGACAATGAA 
GAGAGAATGA GCCGCCTCTC AAAGGTGGCG CCAGTGATTA AAGCCAGAAT GATGGAGTAT 
GGGACCACAA TGGTCAGCTA CCAACCCTTA GGAGATAAGG TCAACTTCTT CCGCATGGTC 
ATCTCAAACC CTGCAGCAAC TCACCAAGAC ATTGACTTCC TCATTGAAGA AATCGAACGC 
CTGGGACAAG ATTTGTAA