Aldh1a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aldh1a1
Entrez Gene ID	24188
Full Name	aldehyde dehydrogenase 1 family, member A1
Synonyms	Ahd2,Aldh1,Aldh2
General protein information	Preferred Names aldehyde dehydrogenase 1 family, member A1 Names retinal dehydrogenase 1 ALDH-E1 ALHDII RALDH 1 aldehyde dehydrogenase 1, subfamily A1 aldehyde dehydrogenase 1A1 aldehyde dehydrogenase 2 aldehyde dehydrogenase family 1 member A1 aldehyde dehydrogenase family 1, subfamily A1 aldehyde dehydrogenase, cytosolic ralDH1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q51
Summary	ubiquitous enzyme located in virtually all mammalian tissues; catalyzes oxidation of aldehyde substrates to carboxylic acids; detoxifies ethanol-derived acetaldehyde [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022407.3	NP_071852.2	retinal dehydrogenase 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ALDH1A1	NP_776664.1
Gallus gallus (chicken)	ALDH1A1	NP_989908.1
Mus musculus (house mouse)	Aldh1a1	NP_038495.2
Rattus norvegicus (Norway rat)	Aldh1a1	NP_071852.2
Oryza sativa (rice)	Os01g0591000	NP_001043453.1
Canis lupus familiaris (dog)	ALDH1A1	XP_533525.1
Oryza sativa (rice)	Os01g0591300	NP_001043454.1
Homo sapiens (human)	ALDH1A1	NP_000680.2
Eremothecium gossypii	AGOS_ADR417W	NP_984512.2
Arabidopsis thaliana (thale cress)	ALDH2C4	NP_566749.1
Pan troglodytes (chimpanzee)	ALDH1A1	NP_001233476.1
	ALDH1A1	XP_001097604.2
Xenopus tropicalis (tropical clawed frog)	aldh1a1	NP_001027486.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of retinoic acid on midkine gene expression in rat anterior pituitary cells.
Maliza R, Fujiwara K, Azuma M, Kikuchi M, Yashiro T
Endocrine journal64(6)633-638(2017 Jun)

Diabetes Impairs the Aldehyde Detoxifying Capacity of the Retina.
McDowell RE, McGahon MK, Augustine J, Chen M, McGeown JG, Curtis TM
Investigative ophthalmology & visual science57(11)4762-71(2016 09)

Development of a high-throughput in vitro assay to identify selective inhibitors for human ALDH1A1.
Morgan CA, Hurley TD
Chemico-biological interactions23429-37(2015 Jun)

Retinaldehyde dehydrogenase 1 regulates a thermogenic program in white adipose tissue.
Kiefer FW, Vernochet C, O'Brien P, Spoerl S, Brown JD, Nallamshetty S, Zeyda M, Stulnig TM, Cohen DE, Kahn CR, Plutzky J
Nature medicine18(6)918-25(2012 Jun)

The hepatic Raldh1 expression is elevated in Zucker fatty rats and its over-expression introduced the retinal-induced Srebp-1c expression in INS-1 cells.
Li Y, Zhang Y, Li R, Chen W, Howell M, Zhang R, Chen G
PloS one7(9)e45210(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

downregulation of ALDH and AKR enzymes, particularly ALDH1a1, may contribute ALE accumulation in the diabetic retina.
Title: Diabetes Impairs the Aldehyde Detoxifying Capacity of the Retina.

Overexpression and inhibition of ALDH1A1 activity results in reduced and increased HNE toxicity, respectively.
Title: Modulation of aldehyde dehydrogenase activity affects (?)-4-hydroxy-2E-nonenal (HNE) toxicity and HNE-protein adduct levels in PC12 cells.

The elevated hepatic expression of Raldh1 in Zucker fatty (obese) rats may cause the excessive retinoic acid production from retinol, and in turn, result in higher Srebp-1c expression.
Title: The hepatic Raldh1 expression is elevated in Zucker fatty rats and its over-expression introduced the retinal-induced Srebp-1c expression in INS-1 cells.

RALDH1 mRNA expression is suppressed by 17beta-estradiol through ERalpha in anterior pituitary gland.
Title: Estrogen receptor alpha regulates retinaldehyde dehydrogenase 1 expression in rat anterior pituitary cells.

RALDH1 mRNA expression is suppressed by estrogen.
Title: Estrogen suppresses retinaldehyde dehydrogenase 1 expression in the anterior pituitary glands of female rats.

The following Aldh1a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aldh1a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04830	NM_022407.3 Latest version!	Rattus norvegicus aldehyde dehydrogenase 1 family, member A1 (Aldh1a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$293.30 $419.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aldh1a1 ( NM_022407.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071852.2 Rattus norvegicus aldehyde dehydrogenase 1 family, member A1 (Aldh1a1), mRNA.

Price & Availability↑

CloneID	ORa04830
Clone ID Related Accession (Same CDS sequence)	NM_022407.3
Accession Version	NM_022407.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	retinal dehydrogenase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF001898.1. On Jun 9, 2003 this sequence version replaced NM_022407.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF001898.1, BC061526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCTTCCC CTGCACAGCC TGCAGTTCCT GCCCCACTGG CCAACTTGAA GATTCAACAC 
ACCAAGATCT TCATAAACAA TGAATGGCAT GATTCAGTGA GTGGCAAGAA ATTTCCTGTC 
CTTAACCCTG CAACTGAGGA GGTCATCTGC CATGTGGAAG AAGGGGACAA GGCAGATGTT 
GACAAAGCTG TGAAGGCCGC AAGACAGGCT TTCCAGATTG GCTCCCCATG GCGCACCATG 
GATGCTTCAG AGAGAGGACG CTTGCTGAAC AAGCTGGCTG ACTTAATGGA GAGAGATCGT 
CTGCTGCTGG CTACAATAGA GGCCATCAAT GGTGGGAAAG TCTTTGCCAA TGCGTACTTG 
TCGGATTTAG GAGGCAGCAT AAAAGCATTA AAGTACTGTG CAGGCTGGGC TGACAAGATC 
CATGGTCAAA CAATACCGAG TGATGGAGAC ATTTTCACTT TTACGAGACG TGAACCTATT 
GGGGTGTGTG GCCAAATCAT CCCTTGGAAT TTTCCACTGC TTATGTTCAT TTGGAAGATA 
GGCCCTGCCC TCAGCTGTGG GAACACGGTG GTCGTCAAGC CAGCAGAGCA AACTCCTCTC 
ACTGCTCTTC ACATGGCATC TTTAATAAAA GAGGCAGGGT TTCCTCCTGG CGTGGTGAAC 
ATTGTCCCTG GTTATGGGCC AACTGCGGGG GCAGCGATCT CCTCTCACAT GGATGTGGAC 
AAGGTGGCCT TCACGGGATC AACCCAGGTT GGCAAATTAA TCAAGGAAGC TGCAGGAAAA 
AGCAATCTGA AGAGGGTCAC GCTGGAGCTT GGGGGAAAGA GCCCTTGCAT TGTGTTTGCA 
GATGCCGACT TGGACATTGC TGTCGAGTTT GCACACCATG GAGTCTTCTA CCATCAAGGC 
CAATGCTGCG TCGCGGCATC CCGGATTTTC GTTGAGGAGT CAGTTTATGA TGAGTTTGTG 
AGAAAGAGTG TTGAGCGAGC CAAGAAATAT GTCCTTGGAA ACCCTCTGAC CCAAGGAATA 
AATCAGGGCC CTCAGATTGA CAAGGAGCAA CATGATAAAA TCCTTGATCT CATTGAGAGT 
GGGAAGAAAG AAGGAGCCAA ACTGGAGTGT GGTGGAGGAC GCTGGGGGAA CAAAGGCTTC 
TTTGTCCAGC CCACAGTCTT CTCCAATGTG ACCGATGAGA TGCGCATTGC CAAAGAGGAG 
ATATTTGGAC CAGTGCAACA AATCATGAAG TTTAAGTCCA TAGATGATGT GATCAAGAGA 
GCAAACAATA CTACCTATGG TCTAGCAGCA GGAGTCTTCA CAAAAGACCT GGACAGGGCC 
ATCACTGTGT CTTCTGCTCT GCAGGCCGGG GTAGTGTGGG TTAACTGCTA TATGATCTTG 
TCAGCCCAGT GCCCCTTCGG TGGATTCAAG ATGTCTGGAA ATGGACGAGA ACTGGGTGAA 
CATGGTCTTT ATGAGTACAC TGAGCTCAAG ACAGTTGCAA TGAAAATATC TCAGAAGAAC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071852.2
CDS	29..1534
Translation	MSSPAQPAVPAPLANLKIQHTKIFINNEWHDSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQAFQIGSPWRTMDASERGRLLNKLADLMERDRLLLATIEAINGGKVFANAYLSDLGGSIKALKYCAGWADKIHGQTIPSDGDIFTFTRREPIGVCGQIIPWNFPLLMFIWKIGPALSCGNTVVVKPAEQTPLTALHMASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTQVGKLIKEAAGKSNLKRVTLELGGKSPCIVFADADLDIAVEFAHHGVFYHQGQCCVAASRIFVEESVYDEFVRKSVERAKKYVLGNPLTQGINQGPQIDKEQHDKILDLIESGKKEGAKLECGGGRWGNKGFFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSIDDVIKRANNTTYGLAAGVFTKDLDRAITVSSALQAGVVWVNCYMILSAQCPFGGFKMSGNGRELGEHGLYEYTELKTVAMKISQKNS

Target ORF information:

RefSeq Version	NM_022407.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aldehyde dehydrogenase 1 family, member A1 (Aldh1a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022407.3

ORF Insert Sequence:

ATGTCTTCCC CTGCACAGCC TGCAGTTCCT GCCCCACTGG CCAACTTGAA GATTCAACAC 
ACCAAGATCT TCATAAACAA TGAATGGCAT GATTCAGTGA GTGGCAAGAA ATTTCCTGTC 
CTTAACCCTG CAACTGAGGA GGTCATCTGC CATGTGGAAG AAGGGGACAA GGCAGATGTT 
GACAAAGCTG TGAAGGCCGC AAGACAGGCT TTCCAGATTG GCTCCCCATG GCGCACCATG 
GATGCTTCAG AGAGAGGACG CTTGCTGAAC AAGCTGGCTG ACTTAATGGA GAGAGATCGT 
CTGCTGCTGG CTACAATAGA GGCCATCAAT GGTGGGAAAG TCTTTGCCAA TGCGTACTTG 
TCGGATTTAG GAGGCAGCAT AAAAGCATTA AAGTACTGTG CAGGCTGGGC TGACAAGATC 
CATGGTCAAA CAATACCGAG TGATGGAGAC ATTTTCACTT TTACGAGACG TGAACCTATT 
GGGGTGTGTG GCCAAATCAT CCCTTGGAAT TTTCCACTGC TTATGTTCAT TTGGAAGATA 
GGCCCTGCCC TCAGCTGTGG GAACACGGTG GTCGTCAAGC CAGCAGAGCA AACTCCTCTC 
ACTGCTCTTC ACATGGCATC TTTAATAAAA GAGGCAGGGT TTCCTCCTGG CGTGGTGAAC 
ATTGTCCCTG GTTATGGGCC AACTGCGGGG GCAGCGATCT CCTCTCACAT GGATGTGGAC 
AAGGTGGCCT TCACGGGATC AACCCAGGTT GGCAAATTAA TCAAGGAAGC TGCAGGAAAA 
AGCAATCTGA AGAGGGTCAC GCTGGAGCTT GGGGGAAAGA GCCCTTGCAT TGTGTTTGCA 
GATGCCGACT TGGACATTGC TGTCGAGTTT GCACACCATG GAGTCTTCTA CCATCAAGGC 
CAATGCTGCG TCGCGGCATC CCGGATTTTC GTTGAGGAGT CAGTTTATGA TGAGTTTGTG 
AGAAAGAGTG TTGAGCGAGC CAAGAAATAT GTCCTTGGAA ACCCTCTGAC CCAAGGAATA 
AATCAGGGCC CTCAGATTGA CAAGGAGCAA CATGATAAAA TCCTTGATCT CATTGAGAGT 
GGGAAGAAAG AAGGAGCCAA ACTGGAGTGT GGTGGAGGAC GCTGGGGGAA CAAAGGCTTC 
TTTGTCCAGC CCACAGTCTT CTCCAATGTG ACCGATGAGA TGCGCATTGC CAAAGAGGAG 
ATATTTGGAC CAGTGCAACA AATCATGAAG TTTAAGTCCA TAGATGATGT GATCAAGAGA 
GCAAACAATA CTACCTATGG TCTAGCAGCA GGAGTCTTCA CAAAAGACCT GGACAGGGCC 
ATCACTGTGT CTTCTGCTCT GCAGGCCGGG GTAGTGTGGG TTAACTGCTA TATGATCTTG 
TCAGCCCAGT GCCCCTTCGG TGGATTCAAG ATGTCTGGAA ATGGACGAGA ACTGGGTGAA 
CATGGTCTTT ATGAGTACAC TGAGCTCAAG ACAGTTGCAA TGAAAATATC TCAGAAGAAC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of retinoic acid on midkine gene expression in rat anterior pituitary cells.
Endocrine journal64(6)633-638(2017 Jun)
Maliza R,Fujiwara K,Azuma M,Kikuchi M,Yashiro T

Diabetes Impairs the Aldehyde Detoxifying Capacity of the Retina.
Investigative ophthalmology & visual science57(11)4762-71(2016 09)
McDowell RE,McGahon MK,Augustine J,Chen M,McGeown JG,Curtis TM

Development of a high-throughput in vitro assay to identify selective inhibitors for human ALDH1A1.
Chemico-biological interactions23429-37(2015 Jun)
Morgan CA,Hurley TD

Retinaldehyde dehydrogenase 1 regulates a thermogenic program in white adipose tissue.
Nature medicine18(6)918-25(2012 Jun)
Kiefer FW,Vernochet C,O'Brien P,Spoerl S,Brown JD,Nallamshetty S,Zeyda M,Stulnig TM,Cohen DE,Kahn CR,Plutzky J

The hepatic Raldh1 expression is elevated in Zucker fatty rats and its over-expression introduced the retinal-induced Srebp-1c expression in INS-1 cells.
PloS one7(9)e45210(2012)
Li Y,Zhang Y,Li R,Chen W,Howell M,Zhang R,Chen G