Acly cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acly
Entrez Gene ID	24159
Full Name	ATP citrate lyase
Synonyms	ACL,Clatp
General protein information	Preferred Names ATP citrate lyase Names ATP-citrate synthase ATP-citrate (pro-S-)-lyase citrate cleavage enzyme
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	catalyzes the synthesis of cytosolic acetyl-CoA; plays a role in lipid and cholesterol metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001111095.1	NP_001104565.1	ATP-citrate synthase isoform 2
NM_016987.2	NP_058683.2	ATP-citrate synthase isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Acly	NP_001186225.1
Gallus gallus (chicken)	ACLY	NP_001025711.1
Danio rerio (zebrafish)	aclya	NP_001002649.1
Caenorhabditis elegans (roundworm)	acly-2	NP_506267.1
Magnaporthe oryzae (rice blast fungus)	MGG_06719	XP_003709430.1
Arabidopsis thaliana (thale cress)	ACLB-2	NP_199757.1
Arabidopsis thaliana (thale cress)	ACLB-1	NP_187317.1
Caenorhabditis elegans (roundworm)	D1005.1	NP_508280.1
Rattus norvegicus (Norway rat)	Acly	NP_058683.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010156	XP_319323.3
Oryza sativa (rice)	Os01g0300200	NP_001042817.1
Xenopus tropicalis (tropical clawed frog)	acly	NP_001008028.1
	ACLY	XP_001108114.2
Bos taurus (cattle)	ACLY	NP_001032534.1
Homo sapiens (human)	ACLY	NP_001087.2
Pan troglodytes (chimpanzee)	ACLY	XP_511495.3
Canis lupus familiaris (dog)	ACLY	XP_850198.1
Drosophila melanogaster (fruit fly)	ATPCL	NP_725514.2
Schizosaccharomyces pombe (fission yeast)	SPBC1703.07	NP_596202.1
Neurospora crassa	NCU06785	XP_963626.1

Gene Ontology
Bibliography

Related articles in PubMed

Enhanced lipid accumulation in the yeast Yarrowia lipolytica by over-expression of ATP:citrate lyase from Mus musculus.
Zhang H, Zhang L, Chen H, Chen YQ, Chen W, Song Y, Ratledge C
Journal of biotechnology192 Pt A78-84(2014 Dec)

Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth.
Lin R, Tao R, Gao X, Li T, Zhou X, Guan KL, Xiong Y, Lei QY
Molecular cell51(4)506-518(2013 Aug)

High level of ATP citrate lyase expression in human and rat pancreatic islets.
MacDonald MJ, Longacre MJ, Warner TF, Thonpho A
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(5)391-3(2013 May)

Differences between human and rodent pancreatic islets: low pyruvate carboxylase, atp citrate lyase, and pyruvate carboxylation and high glucose-stimulated acetoacetate in human pancreatic islets.
MacDonald MJ, Longacre MJ, Stoker SW, Kendrick M, Thonpho A, Brown LJ, Hasan NM, Jitrapakdee S, Fukao T, Hanson MS, Fernandez LA, Odorico J
The Journal of biological chemistry286(21)18383-96(2011 May)

Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: knockdown of SCOT inhibits insulin release in rat insulinoma cells.
Hasan NM, Longacre MJ, Seed Ahmed M, Kendrick MA, Gu H, Ostenson CG, Fukao T, MacDonald MJ
Archives of biochemistry and biophysics499(1-2)62-8(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ATP citrate lyase is important for the pyruvate citrate shuttle and lipid synthesis in insulin secretion.
Title: High level of ATP citrate lyase expression in human and rat pancreatic islets.

Differences between human and rodent pancreatic islets: low pyruvate carboxylase, atp citrate lyase, and pyruvate carboxylation and high glucose-stimulated acetoacetate in human pancreatic islets.
Title: Differences between human and rodent pancreatic islets: low pyruvate carboxylase, atp citrate lyase, and pyruvate carboxylation and high glucose-stimulated acetoacetate in human pancreatic islets.

mRNAs of SCOT and ATP citrate lyase were decreased 55% and 58% and enzyme activities were decreased >70% in pancreatic islets of the GK rat, a model of type 2 diabetes.
Title: Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: knockdown of SCOT inhibits insulin release in rat insulinoma cells.

Normal flux through ATP-citrate lyase is not required for glucose-stimulated insulin secretion.
Title: Normal flux through ATP-citrate lyase or fatty acid synthase is not required for glucose-stimulated insulin secretion.

ATP-citrate lyase is a protein kinase B substrate in adipocytes
Title: The identification of ATP-citrate lyase as a protein kinase B (Akt) substrate in primary adipocytes.

The following Acly gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acly cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12061	NM_001111095.1 Latest version!	Rattus norvegicus ATP citrate lyase (Acly), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$804.30 $1149.00
ORa12088	NM_016987.2 Latest version!	Rattus norvegicus ATP citrate lyase (Acly), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acly ( NM_001111095.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001104565.1 Rattus norvegicus ATP citrate lyase (Acly), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12061
Clone ID Related Accession (Same CDS sequence)	NM_001111095.1
Accession Version	NM_001111095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	ATP-citrate synthase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB800173.1, J05210.1, BC100618.1, DY473160.1 and CR471829.1. Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC100618.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGCCA AGGCAATTTC AGAGCAGACC GGCAAAGAAC TCCTTTACAA GTACATCTGT 
ACCACCTCAG CCATCCAGAA CCGGTTCAAG TATGCCCGGG TTACTCCCGA CACAGACTGG 
GCCCATCTCC TGCAGGACCA CCCCTGGCTG CTTAGCCAGA GCTTGGTAGT CAAGCCGGAC 
CAGCTGATCA AACGTCGAGG AAAGCTTGGT CTAGTCGGGG TCAACCTCTC TCTGGATGGA 
GTCAAATCCT GGCTGAAACC TCGACTGGGA CATGAGGCCA CCGTCGGCAA GGCCAAAGGC 
TTCCTCAAGA ACTTTCTGAT TGAGCCCTTC GTCCCCCACA GTCAGGCGGA GGAGTTCTAC 
GTGTGCATCT ATGCTACCCG GGAAGGAGAC TACGTCCTGT TCCACCATGA AGGGGGTGTG 
GATGTGGGCG ATGTGGACAC CAAAGCCCAG AAGCTGCTTG TGGGTGTGGA CGAGAAACTG 
AACGCTGAAG ACATTAAGAG ACACCTGTTG GTCCACGCCC CCGAAGACAA GAAAGAAATC 
CTGGCCAGCT TCATCTCCGG CCTATTCAAT TTCTACGAAG ATCTTTACTT CACCTACCTT 
GAGATCAACC CCCTTGTGGT GACCAAAGAT GGTGTCTACA TCCTTGACCT GGCGGCCAAG 
GTGGACGCCA CTGCTGACTA CATCTGCAAA GTCAAGTGGG GTGATATAGA GTTCCCTCCC 
CCCTTTGGGC GTGAGGCATA CCCAGAGGAA GCCTACATTG CAGACCTGGA TGCCAAAAGT 
GGGGCGAGCT TGAAGCTGAC CTTGCTGAAC CCCAAGGGGC GGATCTGGAC CATGGTTGCC 
GGGGGTGGCG CCTCTGTCGT GTACAGTGAT ACCATCTGTG ATCTTGGAGG TGTCAACGAA 
CTGGCGAATT ACGGGGAGTA CTCTGGTGCC CCCAGTGAAC AACAGACCTA TGACTACGCC 
AAGACCATCC TCTCACTTAT GACTCGAGAG AAGCACCCGG ATGGCAAGAT CCTCATCATT 
GGAGGCAGCA TTGCAAACTT CACCAACGTG GCCGCCACCT TCAAGGGCAT TGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CCATCTTTGT TCGAAGAGGT 
GGCCCGAACT ATCAAGAGGG ATTACGAGTG ATGGGAGAAG TTGGGAAGAC CACTGGAATC 
CCCATCCATG TCTTTGGCAC AGAAACTCAC ATGACGGCCA TTGTGGGCAT GGCCCTGGGC 
CACCGGCCCA TTCCCAACCA GCCACCCACA GCGGCTCACA CTGCCAACTT CCTCCTTAAT 
GCCAGTGGGA GCACATCGAC ACCAGCACCC AGCAGGACAG CGTCTTTTTC CGAGTCCAGA 
GCTGACGAGG TGGCCCCTGC AAAGAAAGCC AAGCCAGCCA TGCCCCAAGG AAAGAGTGCC 
ACCCTCTTCA GCCGACATAC CAAGGCTATC GTGTGGGGCA TGCAGACCCG GGCTGTGCAA 
GGCATGCTGG ACTTTGACTA CGTGTGCTCC CGAGATGAGC CTTCAGTGGC TGCTATGGTC 
TACCCGTTCA CGGGGGATCA TAAGCAGAAG TTTTACTGGG GACACAAGGA AATCCTGATC 
CCTGTCTTCA AGAACATGGC TGACGCCATG AAAAAGCATC CGGAGGTAGA CGTGCTGATC 
AACTTTGCAT CTCTGCGATC GGCTTATGAC AGCACCATGG AGACCATGAA CTATGCACAG 
ATCCGGACCA TAGCCATCAT AGCAGAAGGC ATCCCTGAGG CTCTCACACG GAAGCTCATC 
AAGAAGGCAG ACCAGAAGGG CGTGACCATC ATTGGGCCAG CCACGGTTGG GGGCATCAAG 
CCTGGATGCT TTAAGATTGG GAATACTGGT GGGATGCTGG ACAACATCCT GGCCTCCAAA 
CTGTATCGCC CAGGCAGTGT GGCCTACGTC TCGCGTTCAG GAGGCATGTC TAACGAACTC 
AATAATATCA TCTCTCGGAC CACAGATGGT GTCTACGAGG GTGTTGCCAT CGGCGGGGAC 
AGGTACCCTG GGTCCACATT CATGGATCAC GTGCTGCGTT ACCAAGACAC TCCAGGAGTC 
AAGATGATTG TAGTTCTTGG GGAGATAGGG GGTACAGAAG AATATAAGAT CTGCCGGGGC 
ATCAAGGAGG GCCGCCTCAC CAAGCCAGTG GTCTGCTGGT GCATCGGGAC CTGTGCCACC 
ATGTTCTCTT CTGAGGTCCA GTTTGGCCAC GCTGGGGCTT GTGCCAACCA GGCTTCTGAA 
ACGGCAGTAG CCAAGAACCA GGCCTTGAAG GAAGCGGGAG TGTTTGTGCC CCGAAGCTTT 
GATGAGCTCG GAGAAATCAT TCAGTCCGTG TATGAAGATC TTGTGGCCAA AGGCGCCATT 
GTACCTGCTC AGGAAGTGCC ACCTCCAACA GTACCCATGG ACTACTCTTG GGCCAGGGAG 
CTGGGTTTAA TCCGAAAACC TGCCTCATTC ATGACCAGCA TCTGTGACGA GCGGGGGCAG 
GAACTCATTT ATGCGGGCAT GCCCATCACC GAGGTCTTCA AGGAAGAGAT GGGCATTGGT 
GGTGTCCTGG GCCTCCTCTG GTTCCAGAGA AGGTTGCCCA AGTATTCCTG CCAGTTCATT 
GAGATGTGTC TCATGGTCAC CGCTGATCAC GGGCCAGCTG TCTCCGGGGC CCATAACACT 
ATCATCTGTG CTCGGGCTGG GAAGGACCTG GTCTCCAGCC TCACCTCAGG GCTGCTCACC 
ATTGGGGACC GGTTTGGGGG TGCCTTGGAC GCAGCAGCGA AGATGTTCAG TAAAGCCTTT 
GACAGCGGCA TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAGGG GAAACTGATC 
ATGGGCATCG GCCATCGAGT CAAATCGATA AACAACCCAG ACATGCGAGT GCAGATCCTC 
AAAGACTTTG TCAAACAGCA CTTCCCCGCC ACCCCGCTGC TCGACTATGC ACTGGAAGTG 
GAGAAAATCA CCACCTCAAA GAAGCCAAAT CTTATCCTGA ACGTGGATGG TTTCATCGGC 
GTTGCGTTTG TGGACATGCT TAGGAACTGT GGCTCCTTCA CCCGGGAGGA AGCTGACGAG 
TATGTTGACA TTGGAGCCCT CAATGGCGTC TTTGTGCTGG GAAGGAGTAT GGGCTTCATC 
GGGCACTATC TTGACCAGAA GAGGCTGAAG CAAGGGCTGT ATCGTCACCC CTGGGACGAC 
ATTTCCTATG TTCTCCCGGA ACACATGAGC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104565.1
CDS	122..3397
Translation	MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLGLVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDTKAQKLLVGVDEKLNAEDIKRHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPVVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVAKGAIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDFVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDMLRNCGSFTREEADEYVDIGALNGVFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Target ORF information:

RefSeq Version	NM_001111095.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP citrate lyase (Acly), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111095.1

ORF Insert Sequence:

ATGTCAGCCA AGGCAATTTC AGAGCAGACC GGCAAAGAAC TCCTTTACAA GTACATCTGT 
ACCACCTCAG CCATCCAGAA CCGGTTCAAG TATGCCCGGG TTACTCCCGA CACAGACTGG 
GCCCATCTCC TGCAGGACCA CCCCTGGCTG CTTAGCCAGA GCTTGGTAGT CAAGCCGGAC 
CAGCTGATCA AACGTCGAGG AAAGCTTGGT CTAGTCGGGG TCAACCTCTC TCTGGATGGA 
GTCAAATCCT GGCTGAAACC TCGACTGGGA CATGAGGCCA CCGTCGGCAA GGCCAAAGGC 
TTCCTCAAGA ACTTTCTGAT TGAGCCCTTC GTCCCCCACA GTCAGGCGGA GGAGTTCTAC 
GTGTGCATCT ATGCTACCCG GGAAGGAGAC TACGTCCTGT TCCACCATGA AGGGGGTGTG 
GATGTGGGCG ATGTGGACAC CAAAGCCCAG AAGCTGCTTG TGGGTGTGGA CGAGAAACTG 
AACGCTGAAG ACATTAAGAG ACACCTGTTG GTCCACGCCC CCGAAGACAA GAAAGAAATC 
CTGGCCAGCT TCATCTCCGG CCTATTCAAT TTCTACGAAG ATCTTTACTT CACCTACCTT 
GAGATCAACC CCCTTGTGGT GACCAAAGAT GGTGTCTACA TCCTTGACCT GGCGGCCAAG 
GTGGACGCCA CTGCTGACTA CATCTGCAAA GTCAAGTGGG GTGATATAGA GTTCCCTCCC 
CCCTTTGGGC GTGAGGCATA CCCAGAGGAA GCCTACATTG CAGACCTGGA TGCCAAAAGT 
GGGGCGAGCT TGAAGCTGAC CTTGCTGAAC CCCAAGGGGC GGATCTGGAC CATGGTTGCC 
GGGGGTGGCG CCTCTGTCGT GTACAGTGAT ACCATCTGTG ATCTTGGAGG TGTCAACGAA 
CTGGCGAATT ACGGGGAGTA CTCTGGTGCC CCCAGTGAAC AACAGACCTA TGACTACGCC 
AAGACCATCC TCTCACTTAT GACTCGAGAG AAGCACCCGG ATGGCAAGAT CCTCATCATT 
GGAGGCAGCA TTGCAAACTT CACCAACGTG GCCGCCACCT TCAAGGGCAT TGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CCATCTTTGT TCGAAGAGGT 
GGCCCGAACT ATCAAGAGGG ATTACGAGTG ATGGGAGAAG TTGGGAAGAC CACTGGAATC 
CCCATCCATG TCTTTGGCAC AGAAACTCAC ATGACGGCCA TTGTGGGCAT GGCCCTGGGC 
CACCGGCCCA TTCCCAACCA GCCACCCACA GCGGCTCACA CTGCCAACTT CCTCCTTAAT 
GCCAGTGGGA GCACATCGAC ACCAGCACCC AGCAGGACAG CGTCTTTTTC CGAGTCCAGA 
GCTGACGAGG TGGCCCCTGC AAAGAAAGCC AAGCCAGCCA TGCCCCAAGG AAAGAGTGCC 
ACCCTCTTCA GCCGACATAC CAAGGCTATC GTGTGGGGCA TGCAGACCCG GGCTGTGCAA 
GGCATGCTGG ACTTTGACTA CGTGTGCTCC CGAGATGAGC CTTCAGTGGC TGCTATGGTC 
TACCCGTTCA CGGGGGATCA TAAGCAGAAG TTTTACTGGG GACACAAGGA AATCCTGATC 
CCTGTCTTCA AGAACATGGC TGACGCCATG AAAAAGCATC CGGAGGTAGA CGTGCTGATC 
AACTTTGCAT CTCTGCGATC GGCTTATGAC AGCACCATGG AGACCATGAA CTATGCACAG 
ATCCGGACCA TAGCCATCAT AGCAGAAGGC ATCCCTGAGG CTCTCACACG GAAGCTCATC 
AAGAAGGCAG ACCAGAAGGG CGTGACCATC ATTGGGCCAG CCACGGTTGG GGGCATCAAG 
CCTGGATGCT TTAAGATTGG GAATACTGGT GGGATGCTGG ACAACATCCT GGCCTCCAAA 
CTGTATCGCC CAGGCAGTGT GGCCTACGTC TCGCGTTCAG GAGGCATGTC TAACGAACTC 
AATAATATCA TCTCTCGGAC CACAGATGGT GTCTACGAGG GTGTTGCCAT CGGCGGGGAC 
AGGTACCCTG GGTCCACATT CATGGATCAC GTGCTGCGTT ACCAAGACAC TCCAGGAGTC 
AAGATGATTG TAGTTCTTGG GGAGATAGGG GGTACAGAAG AATATAAGAT CTGCCGGGGC 
ATCAAGGAGG GCCGCCTCAC CAAGCCAGTG GTCTGCTGGT GCATCGGGAC CTGTGCCACC 
ATGTTCTCTT CTGAGGTCCA GTTTGGCCAC GCTGGGGCTT GTGCCAACCA GGCTTCTGAA 
ACGGCAGTAG CCAAGAACCA GGCCTTGAAG GAAGCGGGAG TGTTTGTGCC CCGAAGCTTT 
GATGAGCTCG GAGAAATCAT TCAGTCCGTG TATGAAGATC TTGTGGCCAA AGGCGCCATT 
GTACCTGCTC AGGAAGTGCC ACCTCCAACA GTACCCATGG ACTACTCTTG GGCCAGGGAG 
CTGGGTTTAA TCCGAAAACC TGCCTCATTC ATGACCAGCA TCTGTGACGA GCGGGGGCAG 
GAACTCATTT ATGCGGGCAT GCCCATCACC GAGGTCTTCA AGGAAGAGAT GGGCATTGGT 
GGTGTCCTGG GCCTCCTCTG GTTCCAGAGA AGGTTGCCCA AGTATTCCTG CCAGTTCATT 
GAGATGTGTC TCATGGTCAC CGCTGATCAC GGGCCAGCTG TCTCCGGGGC CCATAACACT 
ATCATCTGTG CTCGGGCTGG GAAGGACCTG GTCTCCAGCC TCACCTCAGG GCTGCTCACC 
ATTGGGGACC GGTTTGGGGG TGCCTTGGAC GCAGCAGCGA AGATGTTCAG TAAAGCCTTT 
GACAGCGGCA TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAGGG GAAACTGATC 
ATGGGCATCG GCCATCGAGT CAAATCGATA AACAACCCAG ACATGCGAGT GCAGATCCTC 
AAAGACTTTG TCAAACAGCA CTTCCCCGCC ACCCCGCTGC TCGACTATGC ACTGGAAGTG 
GAGAAAATCA CCACCTCAAA GAAGCCAAAT CTTATCCTGA ACGTGGATGG TTTCATCGGC 
GTTGCGTTTG TGGACATGCT TAGGAACTGT GGCTCCTTCA CCCGGGAGGA AGCTGACGAG 
TATGTTGACA TTGGAGCCCT CAATGGCGTC TTTGTGCTGG GAAGGAGTAT GGGCTTCATC 
GGGCACTATC TTGACCAGAA GAGGCTGAAG CAAGGGCTGT ATCGTCACCC CTGGGACGAC 
ATTTCCTATG TTCTCCCGGA ACACATGAGC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acly ( NM_016987.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058683.2 Rattus norvegicus ATP citrate lyase (Acly), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12088
Clone ID Related Accession (Same CDS sequence)	NM_016987.2
Accession Version	NM_016987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	ATP-citrate synthase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB800173.1, J05210.1, BC100618.1, DY473160.1 and CR471829.1. On Dec 11, 2007 this sequence version replaced NM_016987.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05210.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGCCA AGGCAATTTC AGAGCAGACC GGCAAAGAAC TCCTTTACAA GTACATCTGT 
ACCACCTCAG CCATCCAGAA CCGGTTCAAG TATGCCCGGG TTACTCCCGA CACAGACTGG 
GCCCATCTCC TGCAGGACCA CCCCTGGCTG CTTAGCCAGA GCTTGGTAGT CAAGCCGGAC 
CAGCTGATCA AACGTCGAGG AAAGCTTGGT CTAGTCGGGG TCAACCTCTC TCTGGATGGA 
GTCAAATCCT GGCTGAAACC TCGACTGGGA CATGAGGCCA CCGTCGGCAA GGCCAAAGGC 
TTCCTCAAGA ACTTTCTGAT TGAGCCCTTC GTCCCCCACA GTCAGGCGGA GGAGTTCTAC 
GTGTGCATCT ATGCTACCCG GGAAGGAGAC TACGTCCTGT TCCACCATGA AGGGGGTGTG 
GATGTGGGCG ATGTGGACAC CAAAGCCCAG AAGCTGCTTG TGGGTGTGGA CGAGAAACTG 
AACGCTGAAG ACATTAAGAG ACACCTGTTG GTCCACGCCC CCGAAGACAA GAAAGAAATC 
CTGGCCAGCT TCATCTCCGG CCTATTCAAT TTCTACGAAG ATCTTTACTT CACCTACCTT 
GAGATCAACC CCCTTGTGGT GACCAAAGAT GGTGTCTACA TCCTTGACCT GGCGGCCAAG 
GTGGACGCCA CTGCTGACTA CATCTGCAAA GTCAAGTGGG GTGATATAGA GTTCCCTCCC 
CCCTTTGGGC GTGAGGCATA CCCAGAGGAA GCCTACATTG CAGACCTGGA TGCCAAAAGT 
GGGGCGAGCT TGAAGCTGAC CTTGCTGAAC CCCAAGGGGC GGATCTGGAC CATGGTTGCC 
GGGGGTGGCG CCTCTGTCGT GTACAGTGAT ACCATCTGTG ATCTTGGAGG TGTCAACGAA 
CTGGCGAATT ACGGGGAGTA CTCTGGTGCC CCCAGTGAAC AACAGACCTA TGACTACGCC 
AAGACCATCC TCTCACTTAT GACTCGAGAG AAGCACCCGG ATGGCAAGAT CCTCATCATT 
GGAGGCAGCA TTGCAAACTT CACCAACGTG GCCGCCACCT TCAAGGGCAT TGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CCATCTTTGT TCGAAGAGGT 
GGCCCGAACT ATCAAGAGGG ATTACGAGTG ATGGGAGAAG TTGGGAAGAC CACTGGAATC 
CCCATCCATG TCTTTGGCAC AGAAACTCAC ATGACGGCCA TTGTGGGCAT GGCCCTGGGC 
CACCGGCCCA TTCCCAACCA GCCACCCACA GCGGCTCACA CTGCCAACTT CCTCCTTAAT 
GCCAGTGGGA GCACATCGAC ACCAGCACCC AGCAGGACAG CGTCTTTTTC CGAGTCCAGA 
GCTGACGAGG TGGCCCCTGC AAAGAAAGCC AAGCCAGCCA TGCCCCAAGA TTCAGTCCCA 
AGTCCAAGAT CCCTGCAAGG AAAGAGTGCC ACCCTCTTCA GCCGACATAC CAAGGCTATC 
GTGTGGGGCA TGCAGACCCG GGCTGTGCAA GGCATGCTGG ACTTTGACTA CGTGTGCTCC 
CGAGATGAGC CTTCAGTGGC TGCTATGGTC TACCCGTTCA CGGGGGATCA TAAGCAGAAG 
TTTTACTGGG GACACAAGGA AATCCTGATC CCTGTCTTCA AGAACATGGC TGACGCCATG 
AAAAAGCATC CGGAGGTAGA CGTGCTGATC AACTTTGCAT CTCTGCGATC GGCTTATGAC 
AGCACCATGG AGACCATGAA CTATGCACAG ATCCGGACCA TAGCCATCAT AGCAGAAGGC 
ATCCCTGAGG CTCTCACACG GAAGCTCATC AAGAAGGCAG ACCAGAAGGG CGTGACCATC 
ATTGGGCCAG CCACGGTTGG GGGCATCAAG CCTGGATGCT TTAAGATTGG GAATACTGGT 
GGGATGCTGG ACAACATCCT GGCCTCCAAA CTGTATCGCC CAGGCAGTGT GGCCTACGTC 
TCGCGTTCAG GAGGCATGTC TAACGAACTC AATAATATCA TCTCTCGGAC CACAGATGGT 
GTCTACGAGG GTGTTGCCAT CGGCGGGGAC AGGTACCCTG GGTCCACATT CATGGATCAC 
GTGCTGCGTT ACCAAGACAC TCCAGGAGTC AAGATGATTG TAGTTCTTGG GGAGATAGGG 
GGTACAGAAG AATATAAGAT CTGCCGGGGC ATCAAGGAGG GCCGCCTCAC CAAGCCAGTG 
GTCTGCTGGT GCATCGGGAC CTGTGCCACC ATGTTCTCTT CTGAGGTCCA GTTTGGCCAC 
GCTGGGGCTT GTGCCAACCA GGCTTCTGAA ACGGCAGTAG CCAAGAACCA GGCCTTGAAG 
GAAGCGGGAG TGTTTGTGCC CCGAAGCTTT GATGAGCTCG GAGAAATCAT TCAGTCCGTG 
TATGAAGATC TTGTGGCCAA AGGCGCCATT GTACCTGCTC AGGAAGTGCC ACCTCCAACA 
GTACCCATGG ACTACTCTTG GGCCAGGGAG CTGGGTTTAA TCCGAAAACC TGCCTCATTC 
ATGACCAGCA TCTGTGACGA GCGGGGGCAG GAACTCATTT ATGCGGGCAT GCCCATCACC 
GAGGTCTTCA AGGAAGAGAT GGGCATTGGT GGTGTCCTGG GCCTCCTCTG GTTCCAGAGA 
AGGTTGCCCA AGTATTCCTG CCAGTTCATT GAGATGTGTC TCATGGTCAC CGCTGATCAC 
GGGCCAGCTG TCTCCGGGGC CCATAACACT ATCATCTGTG CTCGGGCTGG GAAGGACCTG 
GTCTCCAGCC TCACCTCAGG GCTGCTCACC ATTGGGGACC GGTTTGGGGG TGCCTTGGAC 
GCAGCAGCGA AGATGTTCAG TAAAGCCTTT GACAGCGGCA TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAGGG GAAACTGATC ATGGGCATCG GCCATCGAGT CAAATCGATA 
AACAACCCAG ACATGCGAGT GCAGATCCTC AAAGACTTTG TCAAACAGCA CTTCCCCGCC 
ACCCCGCTGC TCGACTATGC ACTGGAAGTG GAGAAAATCA CCACCTCAAA GAAGCCAAAT 
CTTATCCTGA ACGTGGATGG TTTCATCGGC GTTGCGTTTG TGGACATGCT TAGGAACTGT 
GGCTCCTTCA CCCGGGAGGA AGCTGACGAG TATGTTGACA TTGGAGCCCT CAATGGCGTC 
TTTGTGCTGG GAAGGAGTAT GGGCTTCATC GGGCACTATC TTGACCAGAA GAGGCTGAAG 
CAAGGGCTGT ATCGTCACCC CTGGGACGAC ATTTCCTATG TTCTCCCGGA ACACATGAGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058683.2
CDS	122..3427
Translation	MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLGLVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDTKAQKLLVGVDEKLNAEDIKRHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPVVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVAKGAIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQRRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDFVKQHFPATPLLDYALEVEKITTSKKPNLILNVDGFIGVAFVDMLRNCGSFTREEADEYVDIGALNGVFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Target ORF information:

RefSeq Version	NM_016987.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP citrate lyase (Acly), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016987.2

ORF Insert Sequence:

ATGTCAGCCA AGGCAATTTC AGAGCAGACC GGCAAAGAAC TCCTTTACAA GTACATCTGT 
ACCACCTCAG CCATCCAGAA CCGGTTCAAG TATGCCCGGG TTACTCCCGA CACAGACTGG 
GCCCATCTCC TGCAGGACCA CCCCTGGCTG CTTAGCCAGA GCTTGGTAGT CAAGCCGGAC 
CAGCTGATCA AACGTCGAGG AAAGCTTGGT CTAGTCGGGG TCAACCTCTC TCTGGATGGA 
GTCAAATCCT GGCTGAAACC TCGACTGGGA CATGAGGCCA CCGTCGGCAA GGCCAAAGGC 
TTCCTCAAGA ACTTTCTGAT TGAGCCCTTC GTCCCCCACA GTCAGGCGGA GGAGTTCTAC 
GTGTGCATCT ATGCTACCCG GGAAGGAGAC TACGTCCTGT TCCACCATGA AGGGGGTGTG 
GATGTGGGCG ATGTGGACAC CAAAGCCCAG AAGCTGCTTG TGGGTGTGGA CGAGAAACTG 
AACGCTGAAG ACATTAAGAG ACACCTGTTG GTCCACGCCC CCGAAGACAA GAAAGAAATC 
CTGGCCAGCT TCATCTCCGG CCTATTCAAT TTCTACGAAG ATCTTTACTT CACCTACCTT 
GAGATCAACC CCCTTGTGGT GACCAAAGAT GGTGTCTACA TCCTTGACCT GGCGGCCAAG 
GTGGACGCCA CTGCTGACTA CATCTGCAAA GTCAAGTGGG GTGATATAGA GTTCCCTCCC 
CCCTTTGGGC GTGAGGCATA CCCAGAGGAA GCCTACATTG CAGACCTGGA TGCCAAAAGT 
GGGGCGAGCT TGAAGCTGAC CTTGCTGAAC CCCAAGGGGC GGATCTGGAC CATGGTTGCC 
GGGGGTGGCG CCTCTGTCGT GTACAGTGAT ACCATCTGTG ATCTTGGAGG TGTCAACGAA 
CTGGCGAATT ACGGGGAGTA CTCTGGTGCC CCCAGTGAAC AACAGACCTA TGACTACGCC 
AAGACCATCC TCTCACTTAT GACTCGAGAG AAGCACCCGG ATGGCAAGAT CCTCATCATT 
GGAGGCAGCA TTGCAAACTT CACCAACGTG GCCGCCACCT TCAAGGGCAT TGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CCATCTTTGT TCGAAGAGGT 
GGCCCGAACT ATCAAGAGGG ATTACGAGTG ATGGGAGAAG TTGGGAAGAC CACTGGAATC 
CCCATCCATG TCTTTGGCAC AGAAACTCAC ATGACGGCCA TTGTGGGCAT GGCCCTGGGC 
CACCGGCCCA TTCCCAACCA GCCACCCACA GCGGCTCACA CTGCCAACTT CCTCCTTAAT 
GCCAGTGGGA GCACATCGAC ACCAGCACCC AGCAGGACAG CGTCTTTTTC CGAGTCCAGA 
GCTGACGAGG TGGCCCCTGC AAAGAAAGCC AAGCCAGCCA TGCCCCAAGA TTCAGTCCCA 
AGTCCAAGAT CCCTGCAAGG AAAGAGTGCC ACCCTCTTCA GCCGACATAC CAAGGCTATC 
GTGTGGGGCA TGCAGACCCG GGCTGTGCAA GGCATGCTGG ACTTTGACTA CGTGTGCTCC 
CGAGATGAGC CTTCAGTGGC TGCTATGGTC TACCCGTTCA CGGGGGATCA TAAGCAGAAG 
TTTTACTGGG GACACAAGGA AATCCTGATC CCTGTCTTCA AGAACATGGC TGACGCCATG 
AAAAAGCATC CGGAGGTAGA CGTGCTGATC AACTTTGCAT CTCTGCGATC GGCTTATGAC 
AGCACCATGG AGACCATGAA CTATGCACAG ATCCGGACCA TAGCCATCAT AGCAGAAGGC 
ATCCCTGAGG CTCTCACACG GAAGCTCATC AAGAAGGCAG ACCAGAAGGG CGTGACCATC 
ATTGGGCCAG CCACGGTTGG GGGCATCAAG CCTGGATGCT TTAAGATTGG GAATACTGGT 
GGGATGCTGG ACAACATCCT GGCCTCCAAA CTGTATCGCC CAGGCAGTGT GGCCTACGTC 
TCGCGTTCAG GAGGCATGTC TAACGAACTC AATAATATCA TCTCTCGGAC CACAGATGGT 
GTCTACGAGG GTGTTGCCAT CGGCGGGGAC AGGTACCCTG GGTCCACATT CATGGATCAC 
GTGCTGCGTT ACCAAGACAC TCCAGGAGTC AAGATGATTG TAGTTCTTGG GGAGATAGGG 
GGTACAGAAG AATATAAGAT CTGCCGGGGC ATCAAGGAGG GCCGCCTCAC CAAGCCAGTG 
GTCTGCTGGT GCATCGGGAC CTGTGCCACC ATGTTCTCTT CTGAGGTCCA GTTTGGCCAC 
GCTGGGGCTT GTGCCAACCA GGCTTCTGAA ACGGCAGTAG CCAAGAACCA GGCCTTGAAG 
GAAGCGGGAG TGTTTGTGCC CCGAAGCTTT GATGAGCTCG GAGAAATCAT TCAGTCCGTG 
TATGAAGATC TTGTGGCCAA AGGCGCCATT GTACCTGCTC AGGAAGTGCC ACCTCCAACA 
GTACCCATGG ACTACTCTTG GGCCAGGGAG CTGGGTTTAA TCCGAAAACC TGCCTCATTC 
ATGACCAGCA TCTGTGACGA GCGGGGGCAG GAACTCATTT ATGCGGGCAT GCCCATCACC 
GAGGTCTTCA AGGAAGAGAT GGGCATTGGT GGTGTCCTGG GCCTCCTCTG GTTCCAGAGA 
AGGTTGCCCA AGTATTCCTG CCAGTTCATT GAGATGTGTC TCATGGTCAC CGCTGATCAC 
GGGCCAGCTG TCTCCGGGGC CCATAACACT ATCATCTGTG CTCGGGCTGG GAAGGACCTG 
GTCTCCAGCC TCACCTCAGG GCTGCTCACC ATTGGGGACC GGTTTGGGGG TGCCTTGGAC 
GCAGCAGCGA AGATGTTCAG TAAAGCCTTT GACAGCGGCA TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAGGG GAAACTGATC ATGGGCATCG GCCATCGAGT CAAATCGATA 
AACAACCCAG ACATGCGAGT GCAGATCCTC AAAGACTTTG TCAAACAGCA CTTCCCCGCC 
ACCCCGCTGC TCGACTATGC ACTGGAAGTG GAGAAAATCA CCACCTCAAA GAAGCCAAAT 
CTTATCCTGA ACGTGGATGG TTTCATCGGC GTTGCGTTTG TGGACATGCT TAGGAACTGT 
GGCTCCTTCA CCCGGGAGGA AGCTGACGAG TATGTTGACA TTGGAGCCCT CAATGGCGTC 
TTTGTGCTGG GAAGGAGTAT GGGCTTCATC GGGCACTATC TTGACCAGAA GAGGCTGAAG 
CAAGGGCTGT ATCGTCACCC CTGGGACGAC ATTTCCTATG TTCTCCCGGA ACACATGAGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enhanced lipid accumulation in the yeast Yarrowia lipolytica by over-expression of ATP:citrate lyase from Mus musculus.
Journal of biotechnology192 Pt A78-84(2014 Dec)
Zhang H,Zhang L,Chen H,Chen YQ,Chen W,Song Y,Ratledge C

Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth.
Molecular cell51(4)506-518(2013 Aug)
Lin R,Tao R,Gao X,Li T,Zhou X,Guan KL,Xiong Y,Lei QY

High level of ATP citrate lyase expression in human and rat pancreatic islets.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(5)391-3(2013 May)
MacDonald MJ,Longacre MJ,Warner TF,Thonpho A

Differences between human and rodent pancreatic islets: low pyruvate carboxylase, atp citrate lyase, and pyruvate carboxylation and high glucose-stimulated acetoacetate in human pancreatic islets.
The Journal of biological chemistry286(21)18383-96(2011 May)
MacDonald MJ,Longacre MJ,Stoker SW,Kendrick M,Thonpho A,Brown LJ,Hasan NM,Jitrapakdee S,Fukao T,Hanson MS,Fernandez LA,Odorico J

Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: knockdown of SCOT inhibits insulin release in rat insulinoma cells.
Archives of biochemistry and biophysics499(1-2)62-8(2010 Jul)
Hasan NM,Longacre MJ,Seed Ahmed M,Kendrick MA,Gu H,Ostenson CG,Fukao T,MacDonald MJ