Pkn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkn2
Entrez Gene ID	207122
Full Name	protein kinase N2
Synonyms	Pak-2,Prkcl2
General protein information	Preferred Names protein kinase N2 Names serine/threonine-protein kinase N2 PKN gamma cardiolipin-activated protein kinase Pak2 p140 kinase protease-activated kinase 2 protein kinase C-like 2 protein-kinase C-related kinase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44
Summary	phospholipid-regulated protein kinase, phosphorylates ribosomal protein S6; may play a role in hepatic regulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105755.2	NP_001099225.2	serine/threonine-protein kinase N2
XM_017590603.1	XP_017446092.1	serine/threonine-protein kinase N2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PKN2	XP_001145367.1
	LOC693505	XP_002808270.1
Gallus gallus (chicken)	PKN2	XP_422357.3
Drosophila melanogaster (fruit fly)	Pkn	NP_788292.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007587	XP_308283.5
Xenopus tropicalis (tropical clawed frog)	pkn2	XP_004913783.1
Mus musculus (house mouse)	Pkn2	NP_848769.2
Canis lupus familiaris (dog)	PKN2	XP_547295.4
Homo sapiens (human)	PKN2	NP_006247.1
Bos taurus (cattle)	PKN2	NP_001180171.1
Rattus norvegicus (Norway rat)	Pkn2	NP_001099225.2
Danio rerio (zebrafish)	pkn2	XP_700704.3
Caenorhabditis elegans (roundworm)	pkn-1	NP_001257102.1

Gene Ontology
Bibliography

Related articles in PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration.
Lachmann S, Jevons A, De Rycker M, Casamassima A, Radtke S, Collazos A, Parker PJ
PloS one6(7)e21732(2011)

The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells.
Wallace SW, Magalhaes A, Hall A
Molecular and cellular biology31(1)81-91(2011 Jan)

Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases.
Harrison BC, Huynh K, Lundgaard GL, Helmke SM, Perryman MB, McKinsey TA
FEBS letters584(6)1103-10(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pkn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04273	NM_001105755.2 Latest version!	Rattus norvegicus protein kinase N2 (Pkn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$580.30 $829.00
ORa59603	XM_017590603.1 Latest version!	Rattus norvegicus protein kinase N2 (Pkn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkn2 ( NM_001105755.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099225.2 Rattus norvegicus protein kinase N2 (Pkn2), mRNA.

Price & Availability↑

CloneID	ORa04273
Clone ID Related Accession (Same CDS sequence)	NM_001105755.2
Accession Version	NM_001105755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase N2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07013670.1, AABR07013669.1 and AABR07013668.1. On May 29, 2008 this sequence version replaced NM_001105755.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCCA ACCCCGACCG GGGGGAGATC CTCCTCACGG AGCTGCAGGG AGATTCCCGA 
CCTCTTCCAT TTTCTGAGAA TGTGAGTGCT GTTCAGAAAT TAGACTTTTC AGATACAATA 
GTGCAACAGA AATTGGATGA CGTCAAGGAT CGAATAAAGA GAGAAATAAG GAAAGAACTG 
AAAATCAAAG AAGGAGCTGA AAACCTGAGG AAAGTTACAA CAGATAAGAA AAATTTGGCT 
TATGTAGACA ACATTTTGAA AAAGTCAAAT AAAAAATTAG AAGAATTACA TCACAAGCTA 
CAAGAATTAA ATGCACACAT TGTTGTATCG GATCCAGAGG ACTCTGCAGA TTGTCCGAGG 
ACTCCAGATA CTCCAAATAG TGACTCTCGT AGTTCTACTA GCAACAATAG ATTGATGGCC 
TTACAGAAAC AACTGGATAT AGAACTTAAA GTAAAGCAAG GTGCAGAGAA CATGATACAG 
ATGTACTCAA ATGGCCCTTC AAAGGATCGG AAACTCCATG GTACAGCTCA GCAACTGCTC 
CAGGATAGCA AGACAAAAAT AGAAGTCATC CGAATGCAGA TTCTTCAGGC CGTCCAGACC 
AATGAGCTGG CTTTTGATAA TGCAAAACCT GTGATAAGCC CTCTTGAGCT TCGAATGGAA 
GAATTAAGAC ATCATTTTAG GATAGAATTT GCAGTAGCCG AAGGTGCAAA GAATGTCATG 
AAATTACTTG GCTCAGGAAA AGTAACAGAC AGAAAAGCGC TTTCAGAAGC CCAAGCAAGA 
TTTAATGAAT CAAGTCAGAA GTTGGACCTT TTAAAATATT CACTAGAGCA GAGGTTAAAT 
GAACTCCCCA AGAATCACCC CAAAAGCAGT GTCGTCATTG AGGAGCTCTC CCTTGTTGCA 
TCGCCAACCC TGAGTCCACG ACAGAGTATG CTGTCCACGC AGAACCAGTA CAGCACACTG 
TCCAAGCCAG CAGCACTAAC CGGTACTTTG GAAGTTCGTC TCATGGGGTG CCAAGATATC 
CTGGAGAATG TCCCTGGACG GTCAAAAGCG ACATCCGTAG CACTGCCTGG ATGGAGTCCA 
AGTGAAAACA GATCATCGTT TATGAGCAGA ACTAGTAAAA GTAAAAGTGG AAGCAGTCGC 
AACCTCCTGA AAACCGACGA CTTGTCCAAT GATGTTTGTG CTGTTTTGAA GCTAGATAAT 
ACTGTGGTTG GTCAAACTAT CTGGAAGCCC ATTTCCAATC AGTCGTGGGA CCAGAAGTTT 
ACACTGGAAC TAGACAGGTC ACGTGAACTG GAAATTTCAG TTTATTGGCG TGATTGGAGA 
TCTCTGTGTG CTGTAAAATT TCTGAGGTTA GAAGATTTTT TAGACAACCA GCGGCATGGC 
ATGTGTCTCT ATTTGGAACC ACAGGGTACT TTGTTTGCAG AGGTTACTTT TTTTAATCCA 
GTTATTGAAA GACGACCAAA ACTCCAAAGA CAAAAGAAAA TTTTTTCAAA ACAACAAGGC 
AAAACATTTC TCAGAGCCCC TCAAATGAAT ATTAATATTG CCACTTGGGG AAGGCTAGTG 
AGACGAGCTA TTCCCACAGT AAATCATTCT GGCACGTTCA GCCCTCAGAC TCCTGTGCCC 
GCTACAGTGC CAGTGGTGGA TGCACGCACT CCTGAACTCG CACCTCCAGC CAGTGATTCT 
ACAGTAACCA AATTGGACTT TGATCTTGAA CCTGAAGCTC CTCCAGCCCC ACCACGTGCT 
TCTTCCCTTG GAGAGATAGA TGACTCTTCT GAATTGAGAG TTTTGGATAT ACCTGGACAG 
GGTTCAGAAA CTGTTTTCGA TATTGAGAAT GACAGAAATA ACATGCGTCC AAAATCCAAA 
TCTGAATATG AGCTTAACAT TCCTGACTCA AGTCGAAGCT GTTGGAGTGT TGGAGAACTT 
GAGGACAAGA GATCTCAACA GAGGTTTCAG TTTAATCTAC AAGACTTCAG GTGCTGTGCT 
GTCTTGGGGA GAGGACACTT TGGAAAGGTG CTGTTGGCAG AATATAAACA CACAAATGAA 
ATGTTTGCAA TAAAAGCCTT AAAGAAAGGG GACATTGTGG CACGAGATGA AGTAGACAGC 
TTGATGTGTG AAAAAAGAAT TTTTGAGACT GTGAACAGTG TCCGGCATCC TTTCCTAGTG 
AACCTGTTTG CATGTTTCCA AACCAAAGAG CATGTTTGCT TTGTGATGGA GTACGCTGCT 
GGTGGGGACC TGATGATGCA CATCCACACT GACGTCTTCT CTGAACCCAG AGCTGTATTT 
TATGCAGCAT GTGTAGTTCT TGGATTGCAA TATTTACATG AACACAAAAT TGTTTATAGA 
GACTTGAAGT TGGATAACCT GTTGCTGGAT ACAGAAGGCT TCGTGAAGAT TGCTGACTTT 
GGTCTTTGCA AAGAAGGAAT GGGGTATGGA GATAGGACAA GCACATTTTG TGGTACTCCT 
GAATTTCTTG CCCCAGAAGT ATTAACAGAA ACGTCGTACA CAAGGGCTGT AGATTGGTGG 
GGCCTTGGCG TACTTATATA TGAAATGCTC GTCGGAGAGT CTCCCTTTCC TGGTGATGAT 
GAAGAAGAGG TTTTTGACAG TATTGTAAAT GACGAAGTAA GGTATCCAAG GTTCTTATCT 
ACAGAAGCCA TTTCCATAAT GAGAAGGCTG TTGAGGAGGA ATCCTGAGCG GCGCCTGGGA 
GCTGGTGAGA AAGATGCCGA GGATGTAAAG AAGCACCCGT TTTTCCGGCT AACCGACTGG 
AGTGCACTAC TGGACAAGAA AGTAAAGCCA CCATTTGTAC CTACCATTAG AGGACGGGAA 
GATGTTAGTA ACTTTGATGA TGAATTTACC TCAGAAGCAC CTATTCTGAC TCCCCCTCGA 
GAACCGAGGA TACTCTTGGA GGAGGAGCAG GAGATGTTCC GCGATTTTGA CTACGTTGCT 
GATTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099225.2
CDS	357..3308
Translation	MASNPDRGEILLTELQGDSRPLPFSENVSAVQKLDFSDTIVQQKLDDVKDRIKREIRKELKIKEGAENLRKVTTDKKNLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDSADCPRTPDTPNSDSRSSTSNNRLMALQKQLDIELKVKQGAENMIQMYSNGPSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLNELPKNHPKSSVVIEELSLVASPTLSPRQSMLSTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKATSVALPGWSPSENRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTIWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQTPVPATVPVVDARTPELAPPASDSTVTKLDFDLEPEAPPAPPRASSLGEIDDSSELRVLDIPGQGSETVFDIENDRNNMRPKSKSEYELNIPDSSRSCWSVGELEDKRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKHTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVKKHPFFRLTDWSALLDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEMFRDFDYVADWC

Target ORF information:

RefSeq Version	NM_001105755.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase N2 (Pkn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105755.2

ORF Insert Sequence:

ATGGCGTCCA ACCCCGACCG GGGGGAGATC CTCCTCACGG AGCTGCAGGG AGATTCCCGA 
CCTCTTCCAT TTTCTGAGAA TGTGAGTGCT GTTCAGAAAT TAGACTTTTC AGATACAATA 
GTGCAACAGA AATTGGATGA CGTCAAGGAT CGAATAAAGA GAGAAATAAG GAAAGAACTG 
AAAATCAAAG AAGGAGCTGA AAACCTGAGG AAAGTTACAA CAGATAAGAA AAATTTGGCT 
TATGTAGACA ACATTTTGAA AAAGTCAAAT AAAAAATTAG AAGAATTACA TCACAAGCTA 
CAAGAATTAA ATGCACACAT TGTTGTATCG GATCCAGAGG ACTCTGCAGA TTGTCCGAGG 
ACTCCAGATA CTCCAAATAG TGACTCTCGT AGTTCTACTA GCAACAATAG ATTGATGGCC 
TTACAGAAAC AACTGGATAT AGAACTTAAA GTAAAGCAAG GTGCAGAGAA CATGATACAG 
ATGTACTCAA ATGGCCCTTC AAAGGATCGG AAACTCCATG GTACAGCTCA GCAACTGCTC 
CAGGATAGCA AGACAAAAAT AGAAGTCATC CGAATGCAGA TTCTTCAGGC CGTCCAGACC 
AATGAGCTGG CTTTTGATAA TGCAAAACCT GTGATAAGCC CTCTTGAGCT TCGAATGGAA 
GAATTAAGAC ATCATTTTAG GATAGAATTT GCAGTAGCCG AAGGTGCAAA GAATGTCATG 
AAATTACTTG GCTCAGGAAA AGTAACAGAC AGAAAAGCGC TTTCAGAAGC CCAAGCAAGA 
TTTAATGAAT CAAGTCAGAA GTTGGACCTT TTAAAATATT CACTAGAGCA GAGGTTAAAT 
GAACTCCCCA AGAATCACCC CAAAAGCAGT GTCGTCATTG AGGAGCTCTC CCTTGTTGCA 
TCGCCAACCC TGAGTCCACG ACAGAGTATG CTGTCCACGC AGAACCAGTA CAGCACACTG 
TCCAAGCCAG CAGCACTAAC CGGTACTTTG GAAGTTCGTC TCATGGGGTG CCAAGATATC 
CTGGAGAATG TCCCTGGACG GTCAAAAGCG ACATCCGTAG CACTGCCTGG ATGGAGTCCA 
AGTGAAAACA GATCATCGTT TATGAGCAGA ACTAGTAAAA GTAAAAGTGG AAGCAGTCGC 
AACCTCCTGA AAACCGACGA CTTGTCCAAT GATGTTTGTG CTGTTTTGAA GCTAGATAAT 
ACTGTGGTTG GTCAAACTAT CTGGAAGCCC ATTTCCAATC AGTCGTGGGA CCAGAAGTTT 
ACACTGGAAC TAGACAGGTC ACGTGAACTG GAAATTTCAG TTTATTGGCG TGATTGGAGA 
TCTCTGTGTG CTGTAAAATT TCTGAGGTTA GAAGATTTTT TAGACAACCA GCGGCATGGC 
ATGTGTCTCT ATTTGGAACC ACAGGGTACT TTGTTTGCAG AGGTTACTTT TTTTAATCCA 
GTTATTGAAA GACGACCAAA ACTCCAAAGA CAAAAGAAAA TTTTTTCAAA ACAACAAGGC 
AAAACATTTC TCAGAGCCCC TCAAATGAAT ATTAATATTG CCACTTGGGG AAGGCTAGTG 
AGACGAGCTA TTCCCACAGT AAATCATTCT GGCACGTTCA GCCCTCAGAC TCCTGTGCCC 
GCTACAGTGC CAGTGGTGGA TGCACGCACT CCTGAACTCG CACCTCCAGC CAGTGATTCT 
ACAGTAACCA AATTGGACTT TGATCTTGAA CCTGAAGCTC CTCCAGCCCC ACCACGTGCT 
TCTTCCCTTG GAGAGATAGA TGACTCTTCT GAATTGAGAG TTTTGGATAT ACCTGGACAG 
GGTTCAGAAA CTGTTTTCGA TATTGAGAAT GACAGAAATA ACATGCGTCC AAAATCCAAA 
TCTGAATATG AGCTTAACAT TCCTGACTCA AGTCGAAGCT GTTGGAGTGT TGGAGAACTT 
GAGGACAAGA GATCTCAACA GAGGTTTCAG TTTAATCTAC AAGACTTCAG GTGCTGTGCT 
GTCTTGGGGA GAGGACACTT TGGAAAGGTG CTGTTGGCAG AATATAAACA CACAAATGAA 
ATGTTTGCAA TAAAAGCCTT AAAGAAAGGG GACATTGTGG CACGAGATGA AGTAGACAGC 
TTGATGTGTG AAAAAAGAAT TTTTGAGACT GTGAACAGTG TCCGGCATCC TTTCCTAGTG 
AACCTGTTTG CATGTTTCCA AACCAAAGAG CATGTTTGCT TTGTGATGGA GTACGCTGCT 
GGTGGGGACC TGATGATGCA CATCCACACT GACGTCTTCT CTGAACCCAG AGCTGTATTT 
TATGCAGCAT GTGTAGTTCT TGGATTGCAA TATTTACATG AACACAAAAT TGTTTATAGA 
GACTTGAAGT TGGATAACCT GTTGCTGGAT ACAGAAGGCT TCGTGAAGAT TGCTGACTTT 
GGTCTTTGCA AAGAAGGAAT GGGGTATGGA GATAGGACAA GCACATTTTG TGGTACTCCT 
GAATTTCTTG CCCCAGAAGT ATTAACAGAA ACGTCGTACA CAAGGGCTGT AGATTGGTGG 
GGCCTTGGCG TACTTATATA TGAAATGCTC GTCGGAGAGT CTCCCTTTCC TGGTGATGAT 
GAAGAAGAGG TTTTTGACAG TATTGTAAAT GACGAAGTAA GGTATCCAAG GTTCTTATCT 
ACAGAAGCCA TTTCCATAAT GAGAAGGCTG TTGAGGAGGA ATCCTGAGCG GCGCCTGGGA 
GCTGGTGAGA AAGATGCCGA GGATGTAAAG AAGCACCCGT TTTTCCGGCT AACCGACTGG 
AGTGCACTAC TGGACAAGAA AGTAAAGCCA CCATTTGTAC CTACCATTAG AGGACGGGAA 
GATGTTAGTA ACTTTGATGA TGAATTTACC TCAGAAGCAC CTATTCTGAC TCCCCCTCGA 
GAACCGAGGA TACTCTTGGA GGAGGAGCAG GAGATGTTCC GCGATTTTGA CTACGTTGCT 
GATTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkn2 ( XM_017590603.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446092.1 Rattus norvegicus protein kinase N2 (Pkn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59603
Clone ID Related Accession (Same CDS sequence)	XM_017590603.1
Accession Version	XM_017590603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase N2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTAC AGAAACAACT GGATATAGAA CTTAAAGTAA AGCAAGGTGC AGAGAACATG 
ATACAGATGT ACTCAAATGG CCCTTCAAAG GATCGGAAAC TCCATGGTAC AGCTCAGCAA 
CTGCTCCAGG ATAGCAAGAC AAAAATAGAA GTCATCCGAA TGCAGATTCT TCAGGCCGTC 
CAGACCAATG AGCTGGCTTT TGATAATGCA AAACCTGTGA TAAGCCCTCT TGAGCTTCGA 
ATGGAAGAAT TAAGACATCA TTTTAGGATA GAATTTGCAG TAGCCGAAGG TGCAAAGAAT 
GTCATGAAAT TACTTGGCTC AGGAAAAGTA ACAGACAGAA AAGCGCTTTC AGAAGCCCAA 
GCAAGATTTA ATGAATCAAG TCAGAAGTTG GACCTTTTAA AATATTCACT AGAGCAGAGG 
TTAAATGAAC TCCCCAAGAA TCACCCCAAA AGCAGTGTCG TCATTGAGGA GCTCTCCCTT 
GTTGCATCGC CAACCCTGAG TCCACGACAG AGTATGCTGT CCACGCAGAA CCAGTACAGC 
ACACTGTCCA AGCCAGCAGC ACTAACCGGT ACTTTGGAAG TTCGTCTCAT GGGGTGCCAA 
GATATCCTGG AGAATGTCCC TGGACGGTCA AAAGCGACAT CCGTAGCACT GCCTGGATGG 
AGTCCAAGTG AAAACAGATC ATCGTTTATG AGCAGAACTA GTAAAAGTAA AAGTGGAAGC 
AGTCGCAACC TCCTGAAAAC CGACGACTTG TCCAATGATG TTTGTGCTGT TTTGAAGCTA 
GATAATACTG TGGTTGGTCA AACTATCTGG AAGCCCATTT CCAATCAGTC GTGGGACCAG 
AAGTTTACAC TGGAACTAGA CAGGGTTACT TTTTTTAATC CAGTTATTGA AAGACGACCA 
AAACTCCAAA GACAAAAGAA AATTTTTTCA AAACAACAAG GCAAAACATT TCTCAGAGCC 
CCTCAAATGA ATATTAATAT TGCCACTTGG GGAAGGCTAG TGAGACGAGC TATTCCCACA 
GTAAATCATT CTGGCACGTT CAGCCCTCAG ACTCCTGTGC CCGCTACAGT GCCAGTGGTG 
GATGCACGCA CTCCTGAACT CGCACCTCCA GCCAGTGATT CTACAGTAAC CAAATTGGAC 
TTTGATCTTG AACCTGAAGC TCCTCCAGCC CCACCACGTG CTTCTTCCCT TGGAGAGATA 
GATGACTCTT CTGAATTGAG AGTTTTGGAT ATACCTGGAC AGGGTTCAGA AACTGTTTTC 
GATATTGAGA ATGACAGAAA TAACATGCGT CCAAAATCCA AATCTGAATA TGAGCTTAAC 
ATTCCTGACT CAAGTCGAAG CTGTTGGAGT GTTGGAGAAC TTGAGGACAA GAGATCTCAA 
CAGAGGTTTC AGTTTAATCT ACAAGACTTC AGGTGCTGTG CTGTCTTGGG GAGAGGACAC 
TTTGGAAAGG TGCTGTTGGC AGAATATAAA CACACAAATG AAATGTTTGC AATAAAAGCC 
TTAAAGAAAG GGGACATTGT GGCACGAGAT GAAGTAGACA GCTTGATGTG TGAAAAAAGA 
ATTTTTGAGA CTGTGAACAG TGTCCGGCAT CCTTTCCTAG TGAACCTGTT TGCATGTTTC 
CAAACCAAAG AGCATGTTTG CTTTGTGATG GAGTACGCTG CTGGTGGGGA CCTGATGATG 
CACATCCACA CTGACGTCTT CTCTGAACCC AGAGCTGTAT TTTATGCAGC ATGTGTAGTT 
CTTGGATTGC AATATTTACA TGAACACAAA ATTGTTTATA GAGACTTGAA GTTGGATAAC 
CTGTTGCTGG ATACAGAAGG CTTCGTGAAG ATTGCTGACT TTGGTCTTTG CAAAGAAGGA 
ATGGGGTATG GAGATAGGAC AAGCACATTT TGTGGTACTC CTGAATTTCT TGCCCCAGAA 
GTATTAACAG AAACGTCGTA CACAAGGGCT GTAGATTGGT GGGGCCTTGG CGTACTTATA 
TATGAAATGC TCGTCGGAGA GTCTCCCTTT CCTGGTGATG ATGAAGAAGA GGTTTTTGAC 
AGTATTGTAA ATGACGAAGT AAGGTATCCA AGGTTCTTAT CTACAGAAGC CATTTCCATA 
ATGAGAAGGC TGTTGAGGAG GAATCCTGAG CGGCGCCTGG GAGCTGGTGA GAAAGATGCC 
GAGGATGTAA AGAAGCACCC GTTTTTCCGG CTAACCGACT GGAGTGCACT ACTGGACAAG 
AAAGTAAAGC CACCATTTGT ACCTACCATT AGAGGACGGG AAGATGTTAG TAACTTTGAT 
GATGAATTTA CCTCAGAAGC ACCTATTCTG ACTCCCCCTC GAGAACCGAG GATACTCTTG 
GAGGAGGAGC AGGAGATGTT CCGCGATTTT GACTACGTTG CTGATTGGTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446092.1
CDS	367..2760
Translation	MALQKQLDIELKVKQGAENMIQMYSNGPSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLNELPKNHPKSSVVIEELSLVASPTLSPRQSMLSTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKATSVALPGWSPSENRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTIWKPISNQSWDQKFTLELDRVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQTPVPATVPVVDARTPELAPPASDSTVTKLDFDLEPEAPPAPPRASSLGEIDDSSELRVLDIPGQGSETVFDIENDRNNMRPKSKSEYELNIPDSSRSCWSVGELEDKRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKHTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVKKHPFFRLTDWSALLDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEMFRDFDYVADWC

Target ORF information:

RefSeq Version	XM_017590603.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase N2 (Pkn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590603.1

ORF Insert Sequence:

ATGGCCTTAC AGAAACAACT GGATATAGAA CTTAAAGTAA AGCAAGGTGC AGAGAACATG 
ATACAGATGT ACTCAAATGG CCCTTCAAAG GATCGGAAAC TCCATGGTAC AGCTCAGCAA 
CTGCTCCAGG ATAGCAAGAC AAAAATAGAA GTCATCCGAA TGCAGATTCT TCAGGCCGTC 
CAGACCAATG AGCTGGCTTT TGATAATGCA AAACCTGTGA TAAGCCCTCT TGAGCTTCGA 
ATGGAAGAAT TAAGACATCA TTTTAGGATA GAATTTGCAG TAGCCGAAGG TGCAAAGAAT 
GTCATGAAAT TACTTGGCTC AGGAAAAGTA ACAGACAGAA AAGCGCTTTC AGAAGCCCAA 
GCAAGATTTA ATGAATCAAG TCAGAAGTTG GACCTTTTAA AATATTCACT AGAGCAGAGG 
TTAAATGAAC TCCCCAAGAA TCACCCCAAA AGCAGTGTCG TCATTGAGGA GCTCTCCCTT 
GTTGCATCGC CAACCCTGAG TCCACGACAG AGTATGCTGT CCACGCAGAA CCAGTACAGC 
ACACTGTCCA AGCCAGCAGC ACTAACCGGT ACTTTGGAAG TTCGTCTCAT GGGGTGCCAA 
GATATCCTGG AGAATGTCCC TGGACGGTCA AAAGCGACAT CCGTAGCACT GCCTGGATGG 
AGTCCAAGTG AAAACAGATC ATCGTTTATG AGCAGAACTA GTAAAAGTAA AAGTGGAAGC 
AGTCGCAACC TCCTGAAAAC CGACGACTTG TCCAATGATG TTTGTGCTGT TTTGAAGCTA 
GATAATACTG TGGTTGGTCA AACTATCTGG AAGCCCATTT CCAATCAGTC GTGGGACCAG 
AAGTTTACAC TGGAACTAGA CAGGGTTACT TTTTTTAATC CAGTTATTGA AAGACGACCA 
AAACTCCAAA GACAAAAGAA AATTTTTTCA AAACAACAAG GCAAAACATT TCTCAGAGCC 
CCTCAAATGA ATATTAATAT TGCCACTTGG GGAAGGCTAG TGAGACGAGC TATTCCCACA 
GTAAATCATT CTGGCACGTT CAGCCCTCAG ACTCCTGTGC CCGCTACAGT GCCAGTGGTG 
GATGCACGCA CTCCTGAACT CGCACCTCCA GCCAGTGATT CTACAGTAAC CAAATTGGAC 
TTTGATCTTG AACCTGAAGC TCCTCCAGCC CCACCACGTG CTTCTTCCCT TGGAGAGATA 
GATGACTCTT CTGAATTGAG AGTTTTGGAT ATACCTGGAC AGGGTTCAGA AACTGTTTTC 
GATATTGAGA ATGACAGAAA TAACATGCGT CCAAAATCCA AATCTGAATA TGAGCTTAAC 
ATTCCTGACT CAAGTCGAAG CTGTTGGAGT GTTGGAGAAC TTGAGGACAA GAGATCTCAA 
CAGAGGTTTC AGTTTAATCT ACAAGACTTC AGGTGCTGTG CTGTCTTGGG GAGAGGACAC 
TTTGGAAAGG TGCTGTTGGC AGAATATAAA CACACAAATG AAATGTTTGC AATAAAAGCC 
TTAAAGAAAG GGGACATTGT GGCACGAGAT GAAGTAGACA GCTTGATGTG TGAAAAAAGA 
ATTTTTGAGA CTGTGAACAG TGTCCGGCAT CCTTTCCTAG TGAACCTGTT TGCATGTTTC 
CAAACCAAAG AGCATGTTTG CTTTGTGATG GAGTACGCTG CTGGTGGGGA CCTGATGATG 
CACATCCACA CTGACGTCTT CTCTGAACCC AGAGCTGTAT TTTATGCAGC ATGTGTAGTT 
CTTGGATTGC AATATTTACA TGAACACAAA ATTGTTTATA GAGACTTGAA GTTGGATAAC 
CTGTTGCTGG ATACAGAAGG CTTCGTGAAG ATTGCTGACT TTGGTCTTTG CAAAGAAGGA 
ATGGGGTATG GAGATAGGAC AAGCACATTT TGTGGTACTC CTGAATTTCT TGCCCCAGAA 
GTATTAACAG AAACGTCGTA CACAAGGGCT GTAGATTGGT GGGGCCTTGG CGTACTTATA 
TATGAAATGC TCGTCGGAGA GTCTCCCTTT CCTGGTGATG ATGAAGAAGA GGTTTTTGAC 
AGTATTGTAA ATGACGAAGT AAGGTATCCA AGGTTCTTAT CTACAGAAGC CATTTCCATA 
ATGAGAAGGC TGTTGAGGAG GAATCCTGAG CGGCGCCTGG GAGCTGGTGA GAAAGATGCC 
GAGGATGTAA AGAAGCACCC GTTTTTCCGG CTAACCGACT GGAGTGCACT ACTGGACAAG 
AAAGTAAAGC CACCATTTGT ACCTACCATT AGAGGACGGG AAGATGTTAG TAACTTTGAT 
GATGAATTTA CCTCAGAAGC ACCTATTCTG ACTCCCCCTC GAGAACCGAG GATACTCTTG 
GAGGAGGAGC AGGAGATGTT CCGCGATTTT GACTACGTTG CTGATTGGTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration.
PloS one6(7)e21732(2011)
Lachmann S,Jevons A,De Rycker M,Casamassima A,Radtke S,Collazos A,Parker PJ

The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells.
Molecular and cellular biology31(1)81-91(2011 Jan)
Wallace SW,Magalhaes A,Hall A

Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases.
FEBS letters584(6)1103-10(2010 Mar)
Harrison BC,Huynh K,Lundgaard GL,Helmke SM,Perryman MB,McKinsey TA