Acsl4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acsl4
Entrez Gene ID	113976
Full Name	acyl-CoA synthetase long-chain family member 4
Synonyms	Acs4,Facl4
General protein information	Preferred Names acyl-CoA synthetase long-chain family member 4 Names long-chain-fatty-acid--CoA ligase 4 LACS 4 fatty acid-Coenzyme A ligase, long chain 4 long-chain acyl-CoA synthetase 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq33
Summary	catalyzes the ligation of a fatty acid to produce acyl Co-A; acts on a wide range of fatty acids with preferential activity for arachidonate and eicosapentaenoate; plays a role in fatty acid metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257314.3	XP_006257376.1	long-chain-fatty-acid--CoA ligase 4 isoform X1
XM_006257315.3	XP_006257377.1	long-chain-fatty-acid--CoA ligase 4 isoform X2
NM_053623.1	NP_446075.1	long-chain-fatty-acid--CoA ligase 4
XM_006257316.2	XP_006257378.1	long-chain-fatty-acid--CoA ligase 4 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	acsl4	NP_956943.1
Caenorhabditis elegans (roundworm)	acs-4	NP_498568.1
Canis lupus familiaris (dog)	ACSL4	XP_005641723.1
Caenorhabditis elegans (roundworm)	acs-17	NP_508993.2
Homo sapiens (human)	ACSL4	NP_075266.1
Rattus norvegicus (Norway rat)	Acsl4	NP_446075.1
Xenopus tropicalis (tropical clawed frog)	LOC100494759	XP_004916909.1
Pan troglodytes (chimpanzee)	ACSL4	XP_003317677.1
Mus musculus (house mouse)	Acsl4	NP_997508.1
Gallus gallus (chicken)	ACSL4	XP_004940863.1
Arabidopsis thaliana (thale cress)	LACS9	NP_177882.1
Oryza sativa (rice)	Os12g0168700	NP_001066256.1
	ACSL4	XP_001099465.1
Bos taurus (cattle)	ACSL4	XP_005227959.1

Gene Ontology
Bibliography

Related articles in PubMed

Long-chain acyl-CoA synthetase isoforms differ in preferences for eicosanoid species and long-chain fatty acids.
Klett EL, Chen S, Yechoor A, Lih FB, Coleman RA
Journal of lipid research58(5)884-894(2017 May)

Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells.
Tuohetahuntila M, Spee B, Kruitwagen HS, Wubbolts R, Brouwers JF, van de Lest CH, Molenaar MR, Houweling M, Helms JB, Vaandrager AB
Biochimica et biophysica acta1851(2)220-30(2015 Feb)

Role of long-chain acyl-coenzyme A synthetases in the regulation of arachidonic acid metabolism in interleukin 1?-stimulated rat fibroblasts.
Kuwata H, Yoshimura M, Sasaki Y, Yoda E, Nakatani Y, Kudo I, Hara S
Biochimica et biophysica acta1841(1)44-53(2014 Jan)

Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Ohkuni A, Ohno Y, Kihara A
Biochemical and biophysical research communications442(3-4)195-201(2013 Dec)

Diminished acyl-CoA synthetase isoform 4 activity in INS 832/13 cells reduces cellular epoxyeicosatrienoic acid levels and results in impaired glucose-stimulated insulin secretion.
Klett EL, Chen S, Edin ML, Li LO, Ilkayeva O, Zeldin DC, Newgard CB, Coleman RA
The Journal of biological chemistry288(30)21618-29(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upregulation of ACSL4 is responsible for the increase in triacylglycerol species containing long polyunsaturated fatty acids during activation of hepatic stellate cells.
Title: Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells.

Acsl isozymes play distinct roles in the control of AA remodeling in rat fibroblasts: Acsl4 acts as the first step of enzyme for AA remodeling following IL-1beta stimulation, and Acsl1 is involved in the maintenance of some AA-containing PC species.
Title: Role of long-chain acyl-coenzyme A synthetases in the regulation of arachidonic acid metabolism in interleukin 1?-stimulated rat fibroblasts.

Results suggest that long chain acyl-CoA synthetase Acsl4 activates epoxyeicosatrienoic acids (EETs) to form EET-CoAs that are incorporated into glycerophospholipids, thereby sequestering EETs
Title: Diminished acyl-CoA synthetase isoform 4 activity in INS 832/13 cells reduces cellular epoxyeicosatrienoic acid levels and results in impaired glucose-stimulated insulin secretion.

These results indicate that the ACS4 protein specifically expressed in brain plays an important role in arachidonate metabolism and neuronal differentiation in the brain.
Title: A novel role of brain-type ACS4 isotype in neuronal differentiation.

the involvement of SHP2 activity in the regulation of the expression of the fatty acid-metabolizing enzyme ACSL4
Title: Tyrosine phosphatase SHP2 regulates the expression of acyl-CoA synthetase ACSL4.

The following Acsl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acsl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10115	NM_053623.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa10115	XM_006257315.3 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa10115	XM_006257316.2 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa40111	XM_006257314.3 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acsl4 ( NM_053623.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446075.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), mRNA.

Price & Availability↑

CloneID	ORa10115
Clone ID Related Accession (Same CDS sequence)	XM_006257315.3 , NM_053623.1 , XM_006257316.2
Accession Version	NM_053623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D85189.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D85189.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAAGA GAATAAAAGC TAAGCCCACT TCAGACAAAC CCGGAAGTCC ATATCGCTCT 
GTCACGCACT TCGACTCACT AGCTGTCATA GACATCCCTG GAGCAGATAC TCTGGATAAA 
TTATTTGACC ATGCTGTAGC CAAGTTTGGG AAGAAGGACA GCCTGGGGAC CCGGGAGATC 
CTGAGCGAAG AAAATGAAAT GCAGCCAAAT GGAAAGGTTT TTAAGAAGTT AATTCTTGGG 
AATTATAAAT GGATAAACTA TCTTGAAGTG AACTGCCGAG TGAATAACTT TGGAAGTGGC 
CTCACTGCAC TGGGACTGAA ACCAAAGAAC ACCATTGCCA TTTTCTGTGA GACCAGGGCG 
GAGTGGATGA TTGCAGCGCA GACCTGCTTT AAGTACAACT TTCCGCTTGT GACTTTATAT 
GCTACCCTTG GCAAAGAAGC TGTCGTTCAT GGATTAAATG AGTCTGAGGC TTCCTATCTG 
ATTACTAGTG TTGAACTTCT GGAAAGCAAA CTGAAGGCGG CATTATTAGA TATCAATTGT 
GTTAAACATA TCATTTATGT GGATAACAAG ACTATCAATA GAGCGGAGTA CCCCGAGGGG 
CTTGAGATTC ACAGCATGCA GTCAGTAGAG GAGCTGGGAT CCAAGCCAGA AAACTCAAGC 
ATTCCTCCAA GTAGACCAAC TCCTTCGGAC ATGGCCATTG TCATGTACAC CAGTGGCTCT 
ACTGGCCGCC CCAAGGGAGT GATGATGCAC CATAGCAATC TGATAGCTGG GATGACAGGC 
CAGTGTGAGC GCATACCTGG ATTAGGACCA AAGGACACAT ATATTGGCTA CTTACCTTTG 
GCTCATGTGC TGGAACTGAC AGCAGAGATC TCCTGCTTTA CCTACGGCTG TAGGATTGGA 
TATTCGTCAC CACTCACACT GTCTGACCAG TCCAGCAAAA TCAAGAAGGG AAGCAAGGGT 
GATTGTACTG TACTGAAACC TACACTTATG GCGGCCGTTC CGGAAATCAT GGATAGAATT 
TACAAGAATG TTATGAGCAA GGTTCAAGAG ATGAATTATA TTCAGAAGAC TCTATTTAAA 
ATTGGGTATG ATTATAAATT AGAGCAAATC AAGAAAGGAT ATGATGCCCC TCTTTGTAAT 
CTGATACTGT TTAAGAAGGT GAAGGCTCTT CTAGGAGGAA ATGTCCGCAT GATGCTGTCC 
GGCGGTGCCC CACTGTCCCC CCAGACACAC CGATTCATGA ATGTCTGCTT CTGCTGCCCC 
ATTGGTCAGG GCTATGGGCT GACAGAATCA TGCGGTGCTG GAACAGTTAC TGAAGTTACC 
GACTACACTA CTGGAAGAGT TGGCGCTCCT CTTATTTGCT GTGAAATTAA ACTGAAGGAC 
TGGCAGGAAG GTGGTTATAC AGTTCATGAT AAGCCAAACC CCAGAGGTGA AATTGTGATT 
GGAGGCCAGA ATATCTCCAT GGGATATTTT AAAAATGAAG AGAAAACAGC AGAAGATTAT 
TCTGTGGATG AAAATGGACA AAGGTGGTTT TGCACTGGTG ATATTGGAGA ATTCCACCCT 
GATGGATGCT TACAGATTAT CGATCGTAAG AAAGATCTGG TGAAGCTGCA AGCCGGAGAG 
TACGTGTCTC TTGGGAAAGT CGAAGCCGCA CTGAAGAATT GTCCACTGAT CGACAACATC 
TGTGCTTTTG CCAAAAGTGA CCAGTCTTAT GTGATCAGTT TTGTGGTTCC CAACCAGAAA 
AAGTTGACTC TTTTGGCACA ACAGAAGGGG GTAGAAGGAT CTTGGGTTGA TATTTGCAAT 
AATCCTGCCA TGGAAGCTGA AATACTGAAA GAAATTCGAG AAGCTGCAAA TGCCATGAAA 
TTGGAACGAT TTGAAATTCC AATCAAAGTT CGATTGAGCC CAGAGCCATG GACCCCTGAA 
ACTGGTTTGG TAACAGATGC CTTCAAGCTG AAAAGGAAGG AACTGAAGAA CCATTATCTT 
AAAGACATTG AGCGGATGTA TGGGGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446075.1
CDS	186..2198
Translation	MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVEELGSKPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK

Target ORF information:

RefSeq Version	NM_053623.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053623.1

ORF Insert Sequence:

ATGGCAAAGA GAATAAAAGC TAAGCCCACT TCAGACAAAC CCGGAAGTCC ATATCGCTCT 
GTCACGCACT TCGACTCACT AGCTGTCATA GACATCCCTG GAGCAGATAC TCTGGATAAA 
TTATTTGACC ATGCTGTAGC CAAGTTTGGG AAGAAGGACA GCCTGGGGAC CCGGGAGATC 
CTGAGCGAAG AAAATGAAAT GCAGCCAAAT GGAAAGGTTT TTAAGAAGTT AATTCTTGGG 
AATTATAAAT GGATAAACTA TCTTGAAGTG AACTGCCGAG TGAATAACTT TGGAAGTGGC 
CTCACTGCAC TGGGACTGAA ACCAAAGAAC ACCATTGCCA TTTTCTGTGA GACCAGGGCG 
GAGTGGATGA TTGCAGCGCA GACCTGCTTT AAGTACAACT TTCCGCTTGT GACTTTATAT 
GCTACCCTTG GCAAAGAAGC TGTCGTTCAT GGATTAAATG AGTCTGAGGC TTCCTATCTG 
ATTACTAGTG TTGAACTTCT GGAAAGCAAA CTGAAGGCGG CATTATTAGA TATCAATTGT 
GTTAAACATA TCATTTATGT GGATAACAAG ACTATCAATA GAGCGGAGTA CCCCGAGGGG 
CTTGAGATTC ACAGCATGCA GTCAGTAGAG GAGCTGGGAT CCAAGCCAGA AAACTCAAGC 
ATTCCTCCAA GTAGACCAAC TCCTTCGGAC ATGGCCATTG TCATGTACAC CAGTGGCTCT 
ACTGGCCGCC CCAAGGGAGT GATGATGCAC CATAGCAATC TGATAGCTGG GATGACAGGC 
CAGTGTGAGC GCATACCTGG ATTAGGACCA AAGGACACAT ATATTGGCTA CTTACCTTTG 
GCTCATGTGC TGGAACTGAC AGCAGAGATC TCCTGCTTTA CCTACGGCTG TAGGATTGGA 
TATTCGTCAC CACTCACACT GTCTGACCAG TCCAGCAAAA TCAAGAAGGG AAGCAAGGGT 
GATTGTACTG TACTGAAACC TACACTTATG GCGGCCGTTC CGGAAATCAT GGATAGAATT 
TACAAGAATG TTATGAGCAA GGTTCAAGAG ATGAATTATA TTCAGAAGAC TCTATTTAAA 
ATTGGGTATG ATTATAAATT AGAGCAAATC AAGAAAGGAT ATGATGCCCC TCTTTGTAAT 
CTGATACTGT TTAAGAAGGT GAAGGCTCTT CTAGGAGGAA ATGTCCGCAT GATGCTGTCC 
GGCGGTGCCC CACTGTCCCC CCAGACACAC CGATTCATGA ATGTCTGCTT CTGCTGCCCC 
ATTGGTCAGG GCTATGGGCT GACAGAATCA TGCGGTGCTG GAACAGTTAC TGAAGTTACC 
GACTACACTA CTGGAAGAGT TGGCGCTCCT CTTATTTGCT GTGAAATTAA ACTGAAGGAC 
TGGCAGGAAG GTGGTTATAC AGTTCATGAT AAGCCAAACC CCAGAGGTGA AATTGTGATT 
GGAGGCCAGA ATATCTCCAT GGGATATTTT AAAAATGAAG AGAAAACAGC AGAAGATTAT 
TCTGTGGATG AAAATGGACA AAGGTGGTTT TGCACTGGTG ATATTGGAGA ATTCCACCCT 
GATGGATGCT TACAGATTAT CGATCGTAAG AAAGATCTGG TGAAGCTGCA AGCCGGAGAG 
TACGTGTCTC TTGGGAAAGT CGAAGCCGCA CTGAAGAATT GTCCACTGAT CGACAACATC 
TGTGCTTTTG CCAAAAGTGA CCAGTCTTAT GTGATCAGTT TTGTGGTTCC CAACCAGAAA 
AAGTTGACTC TTTTGGCACA ACAGAAGGGG GTAGAAGGAT CTTGGGTTGA TATTTGCAAT 
AATCCTGCCA TGGAAGCTGA AATACTGAAA GAAATTCGAG AAGCTGCAAA TGCCATGAAA 
TTGGAACGAT TTGAAATTCC AATCAAAGTT CGATTGAGCC CAGAGCCATG GACCCCTGAA 
ACTGGTTTGG TAACAGATGC CTTCAAGCTG AAAAGGAAGG AACTGAAGAA CCATTATCTT 
AAAGACATTG AGCGGATGTA TGGGGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl4 ( XM_006257315.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257377.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa10115
Clone ID Related Accession (Same CDS sequence)	XM_006257315.3 , NM_053623.1 , XM_006257316.2
Accession Version	XM_006257315.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257315.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGA GAATAAAAGC TAAGCCCACT TCAGACAAAC CCGGAAGTCC ATATCGCTCT 
GTCACGCACT TCGACTCACT AGCTGTCATA GACATCCCTG GAGCAGATAC TCTGGATAAA 
TTATTTGACC ATGCTGTAGC CAAGTTTGGG AAGAAGGACA GCCTGGGGAC CCGGGAGATC 
CTGAGCGAAG AAAATGAAAT GCAGCCAAAT GGAAAGGTTT TTAAGAAGTT AATTCTTGGG 
AATTATAAAT GGATAAACTA TCTTGAAGTG AACTGCCGAG TGAATAACTT TGGAAGTGGC 
CTCACTGCAC TGGGACTGAA ACCAAAGAAC ACCATTGCCA TTTTCTGTGA GACCAGGGCG 
GAGTGGATGA TTGCAGCGCA GACCTGCTTT AAGTACAACT TTCCGCTTGT GACTTTATAT 
GCTACCCTTG GCAAAGAAGC TGTCGTTCAT GGATTAAATG AGTCTGAGGC TTCCTATCTG 
ATTACTAGTG TTGAACTTCT GGAAAGCAAA CTGAAGGCGG CATTATTAGA TATCAATTGT 
GTTAAACATA TCATTTATGT GGATAACAAG ACTATCAATA GAGCGGAGTA CCCCGAGGGG 
CTTGAGATTC ACAGCATGCA GTCAGTAGAG GAGCTGGGAT CCAAGCCAGA AAACTCAAGC 
ATTCCTCCAA GTAGACCAAC TCCTTCGGAC ATGGCCATTG TCATGTACAC CAGTGGCTCT 
ACTGGCCGCC CCAAGGGAGT GATGATGCAC CATAGCAATC TGATAGCTGG GATGACAGGC 
CAGTGTGAGC GCATACCTGG ATTAGGACCA AAGGACACAT ATATTGGCTA CTTACCTTTG 
GCTCATGTGC TGGAACTGAC AGCAGAGATC TCCTGCTTTA CCTACGGCTG TAGGATTGGA 
TATTCGTCAC CACTCACACT GTCTGACCAG TCCAGCAAAA TCAAGAAGGG AAGCAAGGGT 
GATTGTACTG TACTGAAACC TACACTTATG GCGGCCGTTC CGGAAATCAT GGATAGAATT 
TACAAGAATG TTATGAGCAA GGTTCAAGAG ATGAATTATA TTCAGAAGAC TCTATTTAAA 
ATTGGGTATG ATTATAAATT AGAGCAAATC AAGAAAGGAT ATGATGCCCC TCTTTGTAAT 
CTGATACTGT TTAAGAAGGT GAAGGCTCTT CTAGGAGGAA ATGTCCGCAT GATGCTGTCC 
GGCGGTGCCC CACTGTCCCC CCAGACACAC CGATTCATGA ATGTCTGCTT CTGCTGCCCC 
ATTGGTCAGG GCTATGGGCT GACAGAATCA TGCGGTGCTG GAACAGTTAC TGAAGTTACC 
GACTACACTA CTGGAAGAGT TGGCGCTCCT CTTATTTGCT GTGAAATTAA ACTGAAGGAC 
TGGCAGGAAG GTGGTTATAC AGTTCATGAT AAGCCAAACC CCAGAGGTGA AATTGTGATT 
GGAGGCCAGA ATATCTCCAT GGGATATTTT AAAAATGAAG AGAAAACAGC AGAAGATTAT 
TCTGTGGATG AAAATGGACA AAGGTGGTTT TGCACTGGTG ATATTGGAGA ATTCCACCCT 
GATGGATGCT TACAGATTAT CGATCGTAAG AAAGATCTGG TGAAGCTGCA AGCCGGAGAG 
TACGTGTCTC TTGGGAAAGT CGAAGCCGCA CTGAAGAATT GTCCACTGAT CGACAACATC 
TGTGCTTTTG CCAAAAGTGA CCAGTCTTAT GTGATCAGTT TTGTGGTTCC CAACCAGAAA 
AAGTTGACTC TTTTGGCACA ACAGAAGGGG GTAGAAGGAT CTTGGGTTGA TATTTGCAAT 
AATCCTGCCA TGGAAGCTGA AATACTGAAA GAAATTCGAG AAGCTGCAAA TGCCATGAAA 
TTGGAACGAT TTGAAATTCC AATCAAAGTT CGATTGAGCC CAGAGCCATG GACCCCTGAA 
ACTGGTTTGG TAACAGATGC CTTCAAGCTG AAAAGGAAGG AACTGAAGAA CCATTATCTT 
AAAGACATTG AGCGGATGTA TGGGGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257377.1
CDS	258..2270
Translation	MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVEELGSKPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK

Target ORF information:

RefSeq Version	XM_006257315.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257315.3

ORF Insert Sequence:

ATGGCAAAGA GAATAAAAGC TAAGCCCACT TCAGACAAAC CCGGAAGTCC ATATCGCTCT 
GTCACGCACT TCGACTCACT AGCTGTCATA GACATCCCTG GAGCAGATAC TCTGGATAAA 
TTATTTGACC ATGCTGTAGC CAAGTTTGGG AAGAAGGACA GCCTGGGGAC CCGGGAGATC 
CTGAGCGAAG AAAATGAAAT GCAGCCAAAT GGAAAGGTTT TTAAGAAGTT AATTCTTGGG 
AATTATAAAT GGATAAACTA TCTTGAAGTG AACTGCCGAG TGAATAACTT TGGAAGTGGC 
CTCACTGCAC TGGGACTGAA ACCAAAGAAC ACCATTGCCA TTTTCTGTGA GACCAGGGCG 
GAGTGGATGA TTGCAGCGCA GACCTGCTTT AAGTACAACT TTCCGCTTGT GACTTTATAT 
GCTACCCTTG GCAAAGAAGC TGTCGTTCAT GGATTAAATG AGTCTGAGGC TTCCTATCTG 
ATTACTAGTG TTGAACTTCT GGAAAGCAAA CTGAAGGCGG CATTATTAGA TATCAATTGT 
GTTAAACATA TCATTTATGT GGATAACAAG ACTATCAATA GAGCGGAGTA CCCCGAGGGG 
CTTGAGATTC ACAGCATGCA GTCAGTAGAG GAGCTGGGAT CCAAGCCAGA AAACTCAAGC 
ATTCCTCCAA GTAGACCAAC TCCTTCGGAC ATGGCCATTG TCATGTACAC CAGTGGCTCT 
ACTGGCCGCC CCAAGGGAGT GATGATGCAC CATAGCAATC TGATAGCTGG GATGACAGGC 
CAGTGTGAGC GCATACCTGG ATTAGGACCA AAGGACACAT ATATTGGCTA CTTACCTTTG 
GCTCATGTGC TGGAACTGAC AGCAGAGATC TCCTGCTTTA CCTACGGCTG TAGGATTGGA 
TATTCGTCAC CACTCACACT GTCTGACCAG TCCAGCAAAA TCAAGAAGGG AAGCAAGGGT 
GATTGTACTG TACTGAAACC TACACTTATG GCGGCCGTTC CGGAAATCAT GGATAGAATT 
TACAAGAATG TTATGAGCAA GGTTCAAGAG ATGAATTATA TTCAGAAGAC TCTATTTAAA 
ATTGGGTATG ATTATAAATT AGAGCAAATC AAGAAAGGAT ATGATGCCCC TCTTTGTAAT 
CTGATACTGT TTAAGAAGGT GAAGGCTCTT CTAGGAGGAA ATGTCCGCAT GATGCTGTCC 
GGCGGTGCCC CACTGTCCCC CCAGACACAC CGATTCATGA ATGTCTGCTT CTGCTGCCCC 
ATTGGTCAGG GCTATGGGCT GACAGAATCA TGCGGTGCTG GAACAGTTAC TGAAGTTACC 
GACTACACTA CTGGAAGAGT TGGCGCTCCT CTTATTTGCT GTGAAATTAA ACTGAAGGAC 
TGGCAGGAAG GTGGTTATAC AGTTCATGAT AAGCCAAACC CCAGAGGTGA AATTGTGATT 
GGAGGCCAGA ATATCTCCAT GGGATATTTT AAAAATGAAG AGAAAACAGC AGAAGATTAT 
TCTGTGGATG AAAATGGACA AAGGTGGTTT TGCACTGGTG ATATTGGAGA ATTCCACCCT 
GATGGATGCT TACAGATTAT CGATCGTAAG AAAGATCTGG TGAAGCTGCA AGCCGGAGAG 
TACGTGTCTC TTGGGAAAGT CGAAGCCGCA CTGAAGAATT GTCCACTGAT CGACAACATC 
TGTGCTTTTG CCAAAAGTGA CCAGTCTTAT GTGATCAGTT TTGTGGTTCC CAACCAGAAA 
AAGTTGACTC TTTTGGCACA ACAGAAGGGG GTAGAAGGAT CTTGGGTTGA TATTTGCAAT 
AATCCTGCCA TGGAAGCTGA AATACTGAAA GAAATTCGAG AAGCTGCAAA TGCCATGAAA 
TTGGAACGAT TTGAAATTCC AATCAAAGTT CGATTGAGCC CAGAGCCATG GACCCCTGAA 
ACTGGTTTGG TAACAGATGC CTTCAAGCTG AAAAGGAAGG AACTGAAGAA CCATTATCTT 
AAAGACATTG AGCGGATGTA TGGGGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl4 ( XM_006257316.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257378.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa10115
Clone ID Related Accession (Same CDS sequence)	XM_006257315.3 , NM_053623.1 , XM_006257316.2
Accession Version	XM_006257316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006257316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGA GAATAAAAGC TAAGCCCACT TCAGACAAAC CCGGAAGTCC ATATCGCTCT 
GTCACGCACT TCGACTCACT AGCTGTCATA GACATCCCTG GAGCAGATAC TCTGGATAAA 
TTATTTGACC ATGCTGTAGC CAAGTTTGGG AAGAAGGACA GCCTGGGGAC CCGGGAGATC 
CTGAGCGAAG AAAATGAAAT GCAGCCAAAT GGAAAGGTTT TTAAGAAGTT AATTCTTGGG 
AATTATAAAT GGATAAACTA TCTTGAAGTG AACTGCCGAG TGAATAACTT TGGAAGTGGC 
CTCACTGCAC TGGGACTGAA ACCAAAGAAC ACCATTGCCA TTTTCTGTGA GACCAGGGCG 
GAGTGGATGA TTGCAGCGCA GACCTGCTTT AAGTACAACT TTCCGCTTGT GACTTTATAT 
GCTACCCTTG GCAAAGAAGC TGTCGTTCAT GGATTAAATG AGTCTGAGGC TTCCTATCTG 
ATTACTAGTG TTGAACTTCT GGAAAGCAAA CTGAAGGCGG CATTATTAGA TATCAATTGT 
GTTAAACATA TCATTTATGT GGATAACAAG ACTATCAATA GAGCGGAGTA CCCCGAGGGG 
CTTGAGATTC ACAGCATGCA GTCAGTAGAG GAGCTGGGAT CCAAGCCAGA AAACTCAAGC 
ATTCCTCCAA GTAGACCAAC TCCTTCGGAC ATGGCCATTG TCATGTACAC CAGTGGCTCT 
ACTGGCCGCC CCAAGGGAGT GATGATGCAC CATAGCAATC TGATAGCTGG GATGACAGGC 
CAGTGTGAGC GCATACCTGG ATTAGGACCA AAGGACACAT ATATTGGCTA CTTACCTTTG 
GCTCATGTGC TGGAACTGAC AGCAGAGATC TCCTGCTTTA CCTACGGCTG TAGGATTGGA 
TATTCGTCAC CACTCACACT GTCTGACCAG TCCAGCAAAA TCAAGAAGGG AAGCAAGGGT 
GATTGTACTG TACTGAAACC TACACTTATG GCGGCCGTTC CGGAAATCAT GGATAGAATT 
TACAAGAATG TTATGAGCAA GGTTCAAGAG ATGAATTATA TTCAGAAGAC TCTATTTAAA 
ATTGGGTATG ATTATAAATT AGAGCAAATC AAGAAAGGAT ATGATGCCCC TCTTTGTAAT 
CTGATACTGT TTAAGAAGGT GAAGGCTCTT CTAGGAGGAA ATGTCCGCAT GATGCTGTCC 
GGCGGTGCCC CACTGTCCCC CCAGACACAC CGATTCATGA ATGTCTGCTT CTGCTGCCCC 
ATTGGTCAGG GCTATGGGCT GACAGAATCA TGCGGTGCTG GAACAGTTAC TGAAGTTACC 
GACTACACTA CTGGAAGAGT TGGCGCTCCT CTTATTTGCT GTGAAATTAA ACTGAAGGAC 
TGGCAGGAAG GTGGTTATAC AGTTCATGAT AAGCCAAACC CCAGAGGTGA AATTGTGATT 
GGAGGCCAGA ATATCTCCAT GGGATATTTT AAAAATGAAG AGAAAACAGC AGAAGATTAT 
TCTGTGGATG AAAATGGACA AAGGTGGTTT TGCACTGGTG ATATTGGAGA ATTCCACCCT 
GATGGATGCT TACAGATTAT CGATCGTAAG AAAGATCTGG TGAAGCTGCA AGCCGGAGAG 
TACGTGTCTC TTGGGAAAGT CGAAGCCGCA CTGAAGAATT GTCCACTGAT CGACAACATC 
TGTGCTTTTG CCAAAAGTGA CCAGTCTTAT GTGATCAGTT TTGTGGTTCC CAACCAGAAA 
AAGTTGACTC TTTTGGCACA ACAGAAGGGG GTAGAAGGAT CTTGGGTTGA TATTTGCAAT 
AATCCTGCCA TGGAAGCTGA AATACTGAAA GAAATTCGAG AAGCTGCAAA TGCCATGAAA 
TTGGAACGAT TTGAAATTCC AATCAAAGTT CGATTGAGCC CAGAGCCATG GACCCCTGAA 
ACTGGTTTGG TAACAGATGC CTTCAAGCTG AAAAGGAAGG AACTGAAGAA CCATTATCTT 
AAAGACATTG AGCGGATGTA TGGGGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257378.1
CDS	329..2341
Translation	MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVEELGSKPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK

Target ORF information:

RefSeq Version	XM_006257316.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257316.2

ORF Insert Sequence:

ATGGCAAAGA GAATAAAAGC TAAGCCCACT TCAGACAAAC CCGGAAGTCC ATATCGCTCT 
GTCACGCACT TCGACTCACT AGCTGTCATA GACATCCCTG GAGCAGATAC TCTGGATAAA 
TTATTTGACC ATGCTGTAGC CAAGTTTGGG AAGAAGGACA GCCTGGGGAC CCGGGAGATC 
CTGAGCGAAG AAAATGAAAT GCAGCCAAAT GGAAAGGTTT TTAAGAAGTT AATTCTTGGG 
AATTATAAAT GGATAAACTA TCTTGAAGTG AACTGCCGAG TGAATAACTT TGGAAGTGGC 
CTCACTGCAC TGGGACTGAA ACCAAAGAAC ACCATTGCCA TTTTCTGTGA GACCAGGGCG 
GAGTGGATGA TTGCAGCGCA GACCTGCTTT AAGTACAACT TTCCGCTTGT GACTTTATAT 
GCTACCCTTG GCAAAGAAGC TGTCGTTCAT GGATTAAATG AGTCTGAGGC TTCCTATCTG 
ATTACTAGTG TTGAACTTCT GGAAAGCAAA CTGAAGGCGG CATTATTAGA TATCAATTGT 
GTTAAACATA TCATTTATGT GGATAACAAG ACTATCAATA GAGCGGAGTA CCCCGAGGGG 
CTTGAGATTC ACAGCATGCA GTCAGTAGAG GAGCTGGGAT CCAAGCCAGA AAACTCAAGC 
ATTCCTCCAA GTAGACCAAC TCCTTCGGAC ATGGCCATTG TCATGTACAC CAGTGGCTCT 
ACTGGCCGCC CCAAGGGAGT GATGATGCAC CATAGCAATC TGATAGCTGG GATGACAGGC 
CAGTGTGAGC GCATACCTGG ATTAGGACCA AAGGACACAT ATATTGGCTA CTTACCTTTG 
GCTCATGTGC TGGAACTGAC AGCAGAGATC TCCTGCTTTA CCTACGGCTG TAGGATTGGA 
TATTCGTCAC CACTCACACT GTCTGACCAG TCCAGCAAAA TCAAGAAGGG AAGCAAGGGT 
GATTGTACTG TACTGAAACC TACACTTATG GCGGCCGTTC CGGAAATCAT GGATAGAATT 
TACAAGAATG TTATGAGCAA GGTTCAAGAG ATGAATTATA TTCAGAAGAC TCTATTTAAA 
ATTGGGTATG ATTATAAATT AGAGCAAATC AAGAAAGGAT ATGATGCCCC TCTTTGTAAT 
CTGATACTGT TTAAGAAGGT GAAGGCTCTT CTAGGAGGAA ATGTCCGCAT GATGCTGTCC 
GGCGGTGCCC CACTGTCCCC CCAGACACAC CGATTCATGA ATGTCTGCTT CTGCTGCCCC 
ATTGGTCAGG GCTATGGGCT GACAGAATCA TGCGGTGCTG GAACAGTTAC TGAAGTTACC 
GACTACACTA CTGGAAGAGT TGGCGCTCCT CTTATTTGCT GTGAAATTAA ACTGAAGGAC 
TGGCAGGAAG GTGGTTATAC AGTTCATGAT AAGCCAAACC CCAGAGGTGA AATTGTGATT 
GGAGGCCAGA ATATCTCCAT GGGATATTTT AAAAATGAAG AGAAAACAGC AGAAGATTAT 
TCTGTGGATG AAAATGGACA AAGGTGGTTT TGCACTGGTG ATATTGGAGA ATTCCACCCT 
GATGGATGCT TACAGATTAT CGATCGTAAG AAAGATCTGG TGAAGCTGCA AGCCGGAGAG 
TACGTGTCTC TTGGGAAAGT CGAAGCCGCA CTGAAGAATT GTCCACTGAT CGACAACATC 
TGTGCTTTTG CCAAAAGTGA CCAGTCTTAT GTGATCAGTT TTGTGGTTCC CAACCAGAAA 
AAGTTGACTC TTTTGGCACA ACAGAAGGGG GTAGAAGGAT CTTGGGTTGA TATTTGCAAT 
AATCCTGCCA TGGAAGCTGA AATACTGAAA GAAATTCGAG AAGCTGCAAA TGCCATGAAA 
TTGGAACGAT TTGAAATTCC AATCAAAGTT CGATTGAGCC CAGAGCCATG GACCCCTGAA 
ACTGGTTTGG TAACAGATGC CTTCAAGCTG AAAAGGAAGG AACTGAAGAA CCATTATCTT 
AAAGACATTG AGCGGATGTA TGGGGGCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl4 ( XM_006257314.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257376.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa40111
Clone ID Related Accession (Same CDS sequence)	XM_006257314.3
Accession Version	XM_006257314.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257314.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTTA AGCTAAATGT GCTCACCATT GTATTGCTGC CTGTCCACTT GTTAATAACA 
ATATACAGTG CCCTTATATT TATTCCATGG TATTTTCTTA CCAATGCCAA GAAGAAAAAC 
GCTATGGCAA AGAGAATAAA AGCTAAGCCC ACTTCAGACA AACCCGGAAG TCCATATCGC 
TCTGTCACGC ACTTCGACTC ACTAGCTGTC ATAGACATCC CTGGAGCAGA TACTCTGGAT 
AAATTATTTG ACCATGCTGT AGCCAAGTTT GGGAAGAAGG ACAGCCTGGG GACCCGGGAG 
ATCCTGAGCG AAGAAAATGA AATGCAGCCA AATGGAAAGG TTTTTAAGAA GTTAATTCTT 
GGGAATTATA AATGGATAAA CTATCTTGAA GTGAACTGCC GAGTGAATAA CTTTGGAAGT 
GGCCTCACTG CACTGGGACT GAAACCAAAG AACACCATTG CCATTTTCTG TGAGACCAGG 
GCGGAGTGGA TGATTGCAGC GCAGACCTGC TTTAAGTACA ACTTTCCGCT TGTGACTTTA 
TATGCTACCC TTGGCAAAGA AGCTGTCGTT CATGGATTAA ATGAGTCTGA GGCTTCCTAT 
CTGATTACTA GTGTTGAACT TCTGGAAAGC AAACTGAAGG CGGCATTATT AGATATCAAT 
TGTGTTAAAC ATATCATTTA TGTGGATAAC AAGACTATCA ATAGAGCGGA GTACCCCGAG 
GGGCTTGAGA TTCACAGCAT GCAGTCAGTA GAGGAGCTGG GATCCAAGCC AGAAAACTCA 
AGCATTCCTC CAAGTAGACC AACTCCTTCG GACATGGCCA TTGTCATGTA CACCAGTGGC 
TCTACTGGCC GCCCCAAGGG AGTGATGATG CACCATAGCA ATCTGATAGC TGGGATGACA 
GGCCAGTGTG AGCGCATACC TGGATTAGGA CCAAAGGACA CATATATTGG CTACTTACCT 
TTGGCTCATG TGCTGGAACT GACAGCAGAG ATCTCCTGCT TTACCTACGG CTGTAGGATT 
GGATATTCGT CACCACTCAC ACTGTCTGAC CAGTCCAGCA AAATCAAGAA GGGAAGCAAG 
GGTGATTGTA CTGTACTGAA ACCTACACTT ATGGCGGCCG TTCCGGAAAT CATGGATAGA 
ATTTACAAGA ATGTTATGAG CAAGGTTCAA GAGATGAATT ATATTCAGAA GACTCTATTT 
AAAATTGGGT ATGATTATAA ATTAGAGCAA ATCAAGAAAG GATATGATGC CCCTCTTTGT 
AATCTGATAC TGTTTAAGAA GGTGAAGGCT CTTCTAGGAG GAAATGTCCG CATGATGCTG 
TCCGGCGGTG CCCCACTGTC CCCCCAGACA CACCGATTCA TGAATGTCTG CTTCTGCTGC 
CCCATTGGTC AGGGCTATGG GCTGACAGAA TCATGCGGTG CTGGAACAGT TACTGAAGTT 
ACCGACTACA CTACTGGAAG AGTTGGCGCT CCTCTTATTT GCTGTGAAAT TAAACTGAAG 
GACTGGCAGG AAGGTGGTTA TACAGTTCAT GATAAGCCAA ACCCCAGAGG TGAAATTGTG 
ATTGGAGGCC AGAATATCTC CATGGGATAT TTTAAAAATG AAGAGAAAAC AGCAGAAGAT 
TATTCTGTGG ATGAAAATGG ACAAAGGTGG TTTTGCACTG GTGATATTGG AGAATTCCAC 
CCTGATGGAT GCTTACAGAT TATCGATCGT AAGAAAGATC TGGTGAAGCT GCAAGCCGGA 
GAGTACGTGT CTCTTGGGAA AGTCGAAGCC GCACTGAAGA ATTGTCCACT GATCGACAAC 
ATCTGTGCTT TTGCCAAAAG TGACCAGTCT TATGTGATCA GTTTTGTGGT TCCCAACCAG 
AAAAAGTTGA CTCTTTTGGC ACAACAGAAG GGGGTAGAAG GATCTTGGGT TGATATTTGC 
AATAATCCTG CCATGGAAGC TGAAATACTG AAAGAAATTC GAGAAGCTGC AAATGCCATG 
AAATTGGAAC GATTTGAAAT TCCAATCAAA GTTCGATTGA GCCCAGAGCC ATGGACCCCT 
GAAACTGGTT TGGTAACAGA TGCCTTCAAG CTGAAAAGGA AGGAACTGAA GAACCATTAT 
CTTAAAGACA TTGAGCGGAT GTATGGGGGC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257376.1
CDS	353..2488
Translation	MKLKLNVLTIVLLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVEELGSKPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK

Target ORF information:

RefSeq Version	XM_006257314.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 4 (Acsl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257314.3

ORF Insert Sequence:

ATGAAACTTA AGCTAAATGT GCTCACCATT GTATTGCTGC CTGTCCACTT GTTAATAACA 
ATATACAGTG CCCTTATATT TATTCCATGG TATTTTCTTA CCAATGCCAA GAAGAAAAAC 
GCTATGGCAA AGAGAATAAA AGCTAAGCCC ACTTCAGACA AACCCGGAAG TCCATATCGC 
TCTGTCACGC ACTTCGACTC ACTAGCTGTC ATAGACATCC CTGGAGCAGA TACTCTGGAT 
AAATTATTTG ACCATGCTGT AGCCAAGTTT GGGAAGAAGG ACAGCCTGGG GACCCGGGAG 
ATCCTGAGCG AAGAAAATGA AATGCAGCCA AATGGAAAGG TTTTTAAGAA GTTAATTCTT 
GGGAATTATA AATGGATAAA CTATCTTGAA GTGAACTGCC GAGTGAATAA CTTTGGAAGT 
GGCCTCACTG CACTGGGACT GAAACCAAAG AACACCATTG CCATTTTCTG TGAGACCAGG 
GCGGAGTGGA TGATTGCAGC GCAGACCTGC TTTAAGTACA ACTTTCCGCT TGTGACTTTA 
TATGCTACCC TTGGCAAAGA AGCTGTCGTT CATGGATTAA ATGAGTCTGA GGCTTCCTAT 
CTGATTACTA GTGTTGAACT TCTGGAAAGC AAACTGAAGG CGGCATTATT AGATATCAAT 
TGTGTTAAAC ATATCATTTA TGTGGATAAC AAGACTATCA ATAGAGCGGA GTACCCCGAG 
GGGCTTGAGA TTCACAGCAT GCAGTCAGTA GAGGAGCTGG GATCCAAGCC AGAAAACTCA 
AGCATTCCTC CAAGTAGACC AACTCCTTCG GACATGGCCA TTGTCATGTA CACCAGTGGC 
TCTACTGGCC GCCCCAAGGG AGTGATGATG CACCATAGCA ATCTGATAGC TGGGATGACA 
GGCCAGTGTG AGCGCATACC TGGATTAGGA CCAAAGGACA CATATATTGG CTACTTACCT 
TTGGCTCATG TGCTGGAACT GACAGCAGAG ATCTCCTGCT TTACCTACGG CTGTAGGATT 
GGATATTCGT CACCACTCAC ACTGTCTGAC CAGTCCAGCA AAATCAAGAA GGGAAGCAAG 
GGTGATTGTA CTGTACTGAA ACCTACACTT ATGGCGGCCG TTCCGGAAAT CATGGATAGA 
ATTTACAAGA ATGTTATGAG CAAGGTTCAA GAGATGAATT ATATTCAGAA GACTCTATTT 
AAAATTGGGT ATGATTATAA ATTAGAGCAA ATCAAGAAAG GATATGATGC CCCTCTTTGT 
AATCTGATAC TGTTTAAGAA GGTGAAGGCT CTTCTAGGAG GAAATGTCCG CATGATGCTG 
TCCGGCGGTG CCCCACTGTC CCCCCAGACA CACCGATTCA TGAATGTCTG CTTCTGCTGC 
CCCATTGGTC AGGGCTATGG GCTGACAGAA TCATGCGGTG CTGGAACAGT TACTGAAGTT 
ACCGACTACA CTACTGGAAG AGTTGGCGCT CCTCTTATTT GCTGTGAAAT TAAACTGAAG 
GACTGGCAGG AAGGTGGTTA TACAGTTCAT GATAAGCCAA ACCCCAGAGG TGAAATTGTG 
ATTGGAGGCC AGAATATCTC CATGGGATAT TTTAAAAATG AAGAGAAAAC AGCAGAAGAT 
TATTCTGTGG ATGAAAATGG ACAAAGGTGG TTTTGCACTG GTGATATTGG AGAATTCCAC 
CCTGATGGAT GCTTACAGAT TATCGATCGT AAGAAAGATC TGGTGAAGCT GCAAGCCGGA 
GAGTACGTGT CTCTTGGGAA AGTCGAAGCC GCACTGAAGA ATTGTCCACT GATCGACAAC 
ATCTGTGCTT TTGCCAAAAG TGACCAGTCT TATGTGATCA GTTTTGTGGT TCCCAACCAG 
AAAAAGTTGA CTCTTTTGGC ACAACAGAAG GGGGTAGAAG GATCTTGGGT TGATATTTGC 
AATAATCCTG CCATGGAAGC TGAAATACTG AAAGAAATTC GAGAAGCTGC AAATGCCATG 
AAATTGGAAC GATTTGAAAT TCCAATCAAA GTTCGATTGA GCCCAGAGCC ATGGACCCCT 
GAAACTGGTT TGGTAACAGA TGCCTTCAAG CTGAAAAGGA AGGAACTGAA GAACCATTAT 
CTTAAAGACA TTGAGCGGAT GTATGGGGGC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Long-chain acyl-CoA synthetase isoforms differ in preferences for eicosanoid species and long-chain fatty acids.
Journal of lipid research58(5)884-894(2017 May)
Klett EL,Chen S,Yechoor A,Lih FB,Coleman RA

Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells.
Biochimica et biophysica acta1851(2)220-30(2015 Feb)
Tuohetahuntila M,Spee B,Kruitwagen HS,Wubbolts R,Brouwers JF,van de Lest CH,Molenaar MR,Houweling M,Helms JB,Vaandrager AB

Role of long-chain acyl-coenzyme A synthetases in the regulation of arachidonic acid metabolism in interleukin 1?-stimulated rat fibroblasts.
Biochimica et biophysica acta1841(1)44-53(2014 Jan)
Kuwata H,Yoshimura M,Sasaki Y,Yoda E,Nakatani Y,Kudo I,Hara S

Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Biochemical and biophysical research communications442(3-4)195-201(2013 Dec)
Ohkuni A,Ohno Y,Kihara A

Diminished acyl-CoA synthetase isoform 4 activity in INS 832/13 cells reduces cellular epoxyeicosatrienoic acid levels and results in impaired glucose-stimulated insulin secretion.
The Journal of biological chemistry288(30)21618-29(2013 Jul)
Klett EL,Chen S,Edin ML,Li LO,Ilkayeva O,Zeldin DC,Newgard CB,Coleman RA